Rroxscaffold_4G00298320

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016087
Physical Location & Seq
Forward (+)
18202262 .. 18204247
1986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00298320.1

Sequence Viewer

Length: 753 bp
ATGGCTCTTCTCGTTCCGTCACCGGAAATCTCCAGAGTCTTCTCTAACCCTAACCCTAACCCTAACCGTAAAGGCTTTCGATTTCCGACGAACTTCGGGTCGAAATCCAGGTCCACGGTTAGGGTTTGCGCTGGGGTTAAGGACAGTGTGGTGAGGAAGTCGGAATTGGATCGGAGTTTAAGGTCGTACGGGCAGTTCTCGGCTCCGGTGAAGCAGGGTCCGAAGCCGAGCAAGGAAGATCAAGAGAAGCAGGAGTACTATGTGAATATGGGGTATGCGATTCGGACTCTGAGGGAGGAGTTTCCCGAACTGTTTTTCAGAGAGCTCAGCCTCGACATTTACAGGGATGATATCACATTCAAAGATCCGATCAATACCTTTATGGGCATTGAGAATTACAAGTTTATATTTTCGGCACTACGTTTCCATGGCCAGATATTTTTCAAGGCCTTGTGGGTTGACGTCATTAGTGTATCGCAGCCGATGGACAATGTTATAATGGTTCGCTGGACTATCCATGGCATGCCACGAGTCCCTTGGGAAAGTCGTGGTCGATTTGATGGAACCTCAGAGTACAAACTTGACAAGAAAGGGAAGATCTATGAGCATCGTGTTGATAACATTGCTCTGAATTCGCCTCCAAAGTTTCAGATTCTGGCCGTAGGCGATATAATGCAATCTCTTGGGTGCCCTTCAACTCCAAGGCCAACATTTTTTGAAACTTCATCATTGCCGTCTTCAAAGAGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

250

Amino Acids

28.77

Weight (kDa)

9.62

Isoelectric Point (pI)

52.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 93 - 203 2.6e-39 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46220
fragaria_vesca FvH4_7g15580
malus_domestica MD01G1073100.v1.1
prunus_persica Prupe.2G179500_v2.0.a1
pyrus_communis pycom01g10210
rosa_chinensis RchiOBHm_Chr1g0358261
rosa_laevigata RLG00000027979
rosa_multiflora Rmu_co8490635.1_g000001 Rmu_sc0002071.1_g000013
rosa_roxburghii Rroxscaffold_4G00298320
rosa_rugosa Rorug01G0259900
rosa_samantha Rh1BG241100 Rh1CG257600 Rh1DG269800
rosa_wichuraiana Rw1G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 497
AatII GACGTC 1 cut(s) 465
AccB1I GGYRCC 1 cut(s) 687
AclWI GGATC 2 cut(s) 177, 359
AcoI YGGCCR 2 cut(s) 430, 657
AcsI RAATTY 1 cut(s) 631
AcyI GRCGYC 1 cut(s) 462
AfaI GTAC 3 cut(s) 188, 257, 575
AfiI CCNNNNNNNGG 1 cut(s) 120
AgsI TTSAA 5 cut(s) 361, 445, 696, 719, 741
AjnI CCWGG 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 1 cut(s) 325
AluI AGCT 1 cut(s) 325
Alw21I GWGCWC 1 cut(s) 327
AlwI GGATC 2 cut(s) 177, 359
AlwNI CAGNNNCTG 1 cut(s) 655
AoxI GGCC 4 cut(s) 430, 447, 657, 704
ApeKI GCWGC 1 cut(s) 478
ApoI RAATTY 1 cut(s) 631
ArsI GACNNNNNNTTYG 4 cut(s) 83, 95, 115, 127
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 111, 218
AsuHPI GGTGA 3 cut(s) 12, 163, 220
AvaII GGWCC 2 cut(s) 111, 218
BaeGI GKGCMC 1 cut(s) 692
BalI TGGCCA 1 cut(s) 432
BanI GGYRCC 1 cut(s) 687
BanII GRGCYC 1 cut(s) 327
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
BauI CACGAG 1 cut(s) 528
BbsI GAAGAC 2 cut(s) 31, 729
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 490
BccI CCATC 2 cut(s) 478, 554
BceAI ACGGC 2 cut(s) 644, 718
BciT130I CCWGG 1 cut(s) 109
BglII AGATCT 1 cut(s) 597
BisI GCNGC 1 cut(s) 479
BlpI GCTNAGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 480
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 2 cut(s) 111, 218
BmgT120I GGNCC 2 cut(s) 111, 218
BmiI GGNNCC 4 cut(s) 204, 219, 565, 689
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 1 cut(s) 616
BpiI GAAGAC 2 cut(s) 31, 729
BpmI CTGGAG 1 cut(s) 16
Bpu1102I GCTNAGC 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 462
BsaJI CCNNGG 5 cut(s) 114, 427, 517, 536, 701
BsaWI WCCGGW 2 cut(s) 22, 205
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse3DI GCAATG 2 cut(s) 621, 728
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 5 cut(s) 114, 427, 517, 536, 701
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMI GCAATG 2 cut(s) 621, 728
BseMII CTCAG 3 cut(s) 281, 340, 582
BseRI GAGGAG 1 cut(s) 311
BseSI GKGCMC 1 cut(s) 692
BseXI GCAGC 1 cut(s) 490
BseYI CCCAGC 1 cut(s) 131
BshFI GGCC 4 cut(s) 432, 449, 659, 706
BshNI GGYRCC 1 cut(s) 687
BsiHKAI GWGCWC 1 cut(s) 327
BsiSI CCGG 2 cut(s) 23, 206
BsiWI CGTACG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 4 cut(s) 432, 449, 659, 706
Bsp1286I GDGCHC 2 cut(s) 327, 692
Bsp143I GATC 5 cut(s) 169, 238, 364, 369, 597
Bsp1720I GCTNAGC 1 cut(s) 326
Bsp19I CCATGG 2 cut(s) 427, 517
BspANI GGCC 4 cut(s) 432, 449, 659, 706
BspCNI CTCAG 3 cut(s) 282, 339, 581
BspLI GGNNCC 4 cut(s) 204, 219, 565, 689
BspPI GGATC 2 cut(s) 177, 359
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 687
BsrDI GCAATG 2 cut(s) 621, 728
BssECI CCNNGG 5 cut(s) 114, 427, 517, 536, 701
BssMI GATC 5 cut(s) 169, 238, 364, 369, 597
BssNI GRCGYC 1 cut(s) 462
BssSI CACGAG 1 cut(s) 528
BssT1I CCWWGG 4 cut(s) 427, 517, 536, 701
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 4 cut(s) 68, 118, 146, 312
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 462
BstC8I GCNNGC 1 cut(s) 524
BstDEI CTNAG 3 cut(s) 290, 326, 568
BstDSI CCRYGG 3 cut(s) 114, 427, 517
BstF5I GGATG 1 cut(s) 352
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 5 cut(s) 172, 241, 367, 372, 600
BstMBI GATC 5 cut(s) 169, 238, 364, 369, 597
BstNI CCWGG 1 cut(s) 109
BstNSI RCATGY 1 cut(s) 526
BstSCI CCNGG 1 cut(s) 107
BstSLI GKGCMC 1 cut(s) 692
BstV1I GCAGC 1 cut(s) 490
BstV2I GAAGAC 2 cut(s) 31, 729
BstX2I RGATCY 2 cut(s) 364, 597
BstYI RGATCY 2 cut(s) 364, 597
BsuRI GGCC 4 cut(s) 432, 449, 659, 706
BtgI CCRYGG 3 cut(s) 114, 427, 517
BtsCI GGATG 1 cut(s) 352
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 1 cut(s) 524
CaiI CAGNNNCTG 1 cut(s) 655
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 111, 218
Csp6I GTAC 3 cut(s) 187, 256, 574
CviAII CATG 3 cut(s) 428, 518, 523
CviQI GTAC 3 cut(s) 187, 256, 574
DdeI CTNAG 3 cut(s) 290, 326, 568
DpnI GATC 5 cut(s) 171, 240, 366, 371, 599
DpnII GATC 5 cut(s) 169, 238, 364, 369, 597
EaeI YGGCCR 2 cut(s) 430, 657
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 4 cut(s) 427, 517, 536, 701
Eco147I AGGCCT 1 cut(s) 449
Eco24I GRGCYC 1 cut(s) 327
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 111, 218
Eco53kI GAGCTC 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 1 cut(s) 107
EcoRV GATATC 1 cut(s) 352
EcoT14I CCWWGG 4 cut(s) 427, 517, 536, 701
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 4 cut(s) 427, 517, 536, 701
FaeI CATG 3 cut(s) 431, 521, 526
FaqI GGGAC 1 cut(s) 518
FatI CATG 3 cut(s) 427, 517, 522
Fnu4HI GCNGC 1 cut(s) 479
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 479
GlaI GCGC 1 cut(s) 130
GluI GCNGC 1 cut(s) 479
GsaI CCCAGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 16
HaeIII GGCC 4 cut(s) 432, 449, 659, 706
HapII CCGG 2 cut(s) 23, 206
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 462
Hin1II CATG 3 cut(s) 431, 521, 526
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 5 cut(s) 36, 280, 286, 531, 652
HpaII CCGG 2 cut(s) 23, 206
HphI GGTGA 3 cut(s) 12, 163, 220
Hpy166II GTNNAC 2 cut(s) 114, 460
Hpy188III TCNNGA 3 cut(s) 33, 242, 305
Hpy8I GTNNAC 2 cut(s) 114, 460
Hpy99I CGWCG 1 cut(s) 91
HpyAV CCTTC 1 cut(s) 702
HpyCH4III ACNGT 4 cut(s) 68, 118, 146, 312
HpyCH4IV ACGT 2 cut(s) 421, 462
HpyCH4V TGCA 1 cut(s) 676
HpyF3I CTNAG 3 cut(s) 290, 326, 568
HpySE526I ACGT 2 cut(s) 421, 462
Hsp92I GRCGYC 1 cut(s) 462
Hsp92II CATG 3 cut(s) 431, 521, 526
HspAI GCGC 1 cut(s) 129
Kzo9I GATC 5 cut(s) 169, 238, 364, 369, 597
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 1 cut(s) 616
MaeII ACGT 2 cut(s) 421, 462
MaeIII GTNAC 1 cut(s) 18
MalI GATC 5 cut(s) 171, 240, 366, 371, 599
MboI GATC 5 cut(s) 169, 238, 364, 369, 597
MboII GAAGA 4 cut(s) 31, 248, 607, 729
MflI RGATCY 2 cut(s) 364, 597
MhlI GDGCHC 2 cut(s) 327, 692
MlsI TGGCCA 1 cut(s) 432
MluCI AATT 3 cut(s) 164, 394, 631
MluNI TGGCCA 1 cut(s) 432
MlyI GAGTC 3 cut(s) 45, 280, 540
MmeI TCCRAC 2 cut(s) 110, 141
MnlI CCTC 7 cut(s) 147, 285, 289, 341, 577, 648, 738
Mox20I TGGCCA 1 cut(s) 432
MscI TGGCCA 1 cut(s) 432
MseI TTAA 2 cut(s) 138, 179
Msp20I TGGCCA 1 cut(s) 432
MspI CCGG 2 cut(s) 23, 206
MspR9I CCNGG 1 cut(s) 109
MvaI CCWGG 1 cut(s) 109
NcoI CCATGG 2 cut(s) 427, 517
NdeII GATC 5 cut(s) 169, 238, 364, 369, 597
NlaIII CATG 3 cut(s) 431, 521, 526
NlaIV GGNNCC 4 cut(s) 204, 219, 565, 689
NmeAIII GCCGAG 2 cut(s) 179, 252
NmuCI GTSAC 1 cut(s) 18
NspI RCATGY 1 cut(s) 526
PaeI GCATGC 1 cut(s) 526
PceI AGGCCT 1 cut(s) 449
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 2 cut(s) 280, 652
Pfl23II CGTACG 1 cut(s) 186
PkrI GCNGC 1 cut(s) 480
PleI GAGTC 3 cut(s) 44, 280, 539
PpsI GAGTC 3 cut(s) 44, 280, 539
PsiI TTATAA 1 cut(s) 497
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 107
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 1 cut(s) 107
PspLI CGTACG 1 cut(s) 186
PspN4I GGNNCC 4 cut(s) 204, 219, 565, 689
PspPI GGNCC 2 cut(s) 111, 218
PsrI GAACNNNNNNTAC 2 cut(s) 179, 211
PstNI CAGNNNCTG 1 cut(s) 655
PsuI RGATCY 2 cut(s) 364, 597
RsaI GTAC 3 cut(s) 188, 257, 575
RsaNI GTAC 3 cut(s) 187, 256, 574
SacI GAGCTC 1 cut(s) 327
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 138, 179
SatI GCNGC 1 cut(s) 479
Sau3AI GATC 5 cut(s) 169, 238, 364, 369, 597
Sau96I GGNCC 2 cut(s) 111, 218
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 3 cut(s) 45, 280, 540
ScrFI CCNGG 1 cut(s) 109
SduI GDGCHC 2 cut(s) 327, 692
SetI ASST 7 cut(s) 113, 185, 327, 380, 424, 465, 569
SfaNI GCATC 1 cut(s) 616
SinI GGWCC 2 cut(s) 111, 218
SphI GCATGC 1 cut(s) 526
Sse9I AATT 3 cut(s) 164, 394, 631
SseBI AGGCCT 1 cut(s) 449
SstI GAGCTC 1 cut(s) 327
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 107
StyI CCWWGG 4 cut(s) 427, 517, 536, 701
TaaI ACNGT 4 cut(s) 68, 118, 146, 312
TaiI ACGT 2 cut(s) 424, 465
TaqI TCGA 4 cut(s) 79, 101, 333, 553
TasI AATT 3 cut(s) 164, 394, 631
TatI WGTACW 2 cut(s) 255, 573
TfiI GAWTC 2 cut(s) 280, 652
Tru1I TTAA 2 cut(s) 138, 179
Tru9I TTAA 2 cut(s) 138, 179
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 1 cut(s) 478
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 1 cut(s) 714
TspGWI ACGGA 1 cut(s) 6
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 2 cut(s) 111, 218
XapI RAATTY 1 cut(s) 631
XceI RCATGY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 534
ZraI GACGTC 1 cut(s) 463
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.