Rh1CG257600

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0016087
Physical Location & Seq
Reverse (-)
52192970 .. 52198123
5154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG257600.1

Sequence Viewer

Length: 873 bp
ATGGCTCTTCTCGTTCCGTCACCGGAAATCTCCATAGTCTTCTCTAACCCTAACCCTAACCGTAAAGGCTTTCGAATTCCGACGAACTTCGGGTCGAAATCCAGGTCCACGGTTAGGGTTTGCGCCGGGGTTAAAGACAGTGCAGTGAGCGCGTCGGAATTGGATCGGAATTTAAGGTCGTACGGGCAGTTCTCGGCTCCGGTGAAGCAGGGTCCGAAGCCGAGCAAGGAAGATCAAGAGAAGCAGGAGTACTATGTGAATATGGGGTATGCGATTCGGACTCTGAGGGAGGAGTTTCCCGAGCTGTTTTTCAGAGAGCTTAGCCTCGACATTTACAGGGATGATATCACATTCAAAGATCCGATCAATACCTTTATGGGCATTGAGAATTACAAGTCTATATTTTCGGCACTACGTTTCCATGGCCAGATATTTTTCAAGGCCTTGTGGGTTGACGTCATTAGTGTATCGCAGCCGATGGACAATGTTATAATGGTTCGCTGGACTATCCATGGCATGCCACGAGTCCCTTGGGAAAGTCGTGGTCGATTTGATGGAACCTCAGAGTACAAACTTGACAAGAAAGGGAAGATCTATGAGCATCGTGTTGATAACATTGCCCTGAATTCGCCTCCAAAAATTGACATCGACTACTCCAGTGATAAAAAGTTGGTTTACATTGCAAAAAATGTTGCCCAAGTTGACAAGGCACTGCCAGACACTAGCATAGCGGATATTGAATGTACTGATCTGTTAAAACTACTCAGATTCCGAGCTCTGAGTTCAGCTATGTACAATGCTTGCATACTTTGTATAATACATGACTACTACAAGTTAGAAGACAGCAGGAGCATGAGACTGTACGCTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

290

Amino Acids

33.27

Weight (kDa)

8.85

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 91 - 201 3.7e-39 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46220
fragaria_vesca FvH4_7g15580
malus_domestica MD01G1073100.v1.1
prunus_persica Prupe.2G179500_v2.0.a1
pyrus_communis pycom01g10210
rosa_chinensis RchiOBHm_Chr1g0358261
rosa_laevigata RLG00000027979
rosa_multiflora Rmu_co8490635.1_g000001 Rmu_sc0002071.1_g000013
rosa_roxburghii Rroxscaffold_4G00298320
rosa_rugosa Rorug01G0259900
rosa_samantha Rh1BG241100 Rh1CG257600 Rh1DG269800
rosa_wichuraiana Rw1G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 491
AatII GACGTC 1 cut(s) 459
AccII CGCG 1 cut(s) 152
AciI CCGC 1 cut(s) 731
AclWI GGATC 2 cut(s) 171, 353
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 3 cut(s) 75, 169, 625
AcyI GRCGYC 1 cut(s) 456
AfaI GTAC 6 cut(s) 182, 251, 569, 745, 794, 863
AfiI CCNNNNNNNGG 1 cut(s) 114
AgsI TTSAA 3 cut(s) 355, 439, 740
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 4 cut(s) 304, 319, 776, 788
AluI AGCT 4 cut(s) 304, 319, 776, 788
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 1 cut(s) 850
AlwI GGATC 2 cut(s) 171, 353
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 2 cut(s) 424, 441
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 3 cut(s) 75, 169, 625
ArsI GACNNNNNNTTYG 4 cut(s) 77, 89, 109, 121
AspLEI GCGC 2 cut(s) 125, 152
AspS9I GGNCC 2 cut(s) 105, 212
AsuC2I CCSGG 1 cut(s) 127
AsuHPI GGTGA 2 cut(s) 12, 214
AsuII TTCGAA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 2 cut(s) 105, 212
BalI TGGCCA 1 cut(s) 426
BanII GRGCYC 1 cut(s) 778
BarI GAAGNNNNNNTAC 2 cut(s) 233, 265
BauI CACGAG 1 cut(s) 522
BbsI GAAGAC 2 cut(s) 31, 846
Bbv12I GWGCWC 1 cut(s) 778
BbvI GCAGC 1 cut(s) 484
BccI CCATC 2 cut(s) 472, 548
BciT130I CCWGG 1 cut(s) 103
BcnI CCSGG 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 850
BfaI CTAG 2 cut(s) 723, 871
BglII AGATCT 1 cut(s) 591
BisI GCNGC 1 cut(s) 473
BlpI GCTNAGC 1 cut(s) 320
BlsI GCNGC 1 cut(s) 474
BmcAI AGTACT 1 cut(s) 251
Bme1390I CCNGG 2 cut(s) 103, 127
Bme18I GGWCC 2 cut(s) 105, 212
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 2 cut(s) 105, 212
BmiI GGNNCC 3 cut(s) 198, 213, 559
BmrFI CCNGG 2 cut(s) 103, 127
BmsI GCATC 1 cut(s) 610
BpiI GAAGAC 2 cut(s) 31, 846
BpmI CTGGAG 1 cut(s) 640
Bpu1102I GCTNAGC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 127
BsaHI GRCGYC 1 cut(s) 456
BsaJI CCNNGG 5 cut(s) 108, 126, 421, 511, 530
BsaWI WCCGGW 2 cut(s) 22, 199
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 657
Bse3DI GCAATG 2 cut(s) 615, 678
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 5 cut(s) 108, 126, 421, 511, 530
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMI GCAATG 2 cut(s) 615, 678
BseMII CTCAG 4 cut(s) 275, 576, 770, 778
BseNI ACTGG 1 cut(s) 657
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 1 cut(s) 484
BsgI GTGCAG 1 cut(s) 162
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 2 cut(s) 426, 443
BsiHKAI GWGCWC 1 cut(s) 778
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 3 cut(s) 23, 126, 200
BsiWI CGTACG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 850
BsmFI GGGAC 1 cut(s) 512
BsnI GGCC 2 cut(s) 426, 443
BsoBI CYCGRG 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 778
Bsp1407I TGTACA 1 cut(s) 792
Bsp143I GATC 6 cut(s) 163, 232, 358, 363, 591, 748
Bsp1720I GCTNAGC 1 cut(s) 320
Bsp19I CCATGG 2 cut(s) 421, 511
BspACI CCGC 1 cut(s) 731
BspANI GGCC 2 cut(s) 426, 443
BspCNI CTCAG 4 cut(s) 276, 575, 771, 777
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 3 cut(s) 198, 213, 559
BspPI GGATC 2 cut(s) 171, 353
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 73
BsrDI GCAATG 2 cut(s) 615, 678
BsrGI TGTACA 1 cut(s) 792
BsrI ACTGG 1 cut(s) 657
BssECI CCNNGG 5 cut(s) 108, 126, 421, 511, 530
BssMI GATC 6 cut(s) 163, 232, 358, 363, 591, 748
BssNI GRCGYC 1 cut(s) 456
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 3 cut(s) 421, 511, 530
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 4 cut(s) 62, 112, 140, 861
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 456
BstAUI TGTACA 1 cut(s) 792
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 3 cut(s) 518, 802, 868
BstDEI CTNAG 5 cut(s) 284, 320, 562, 764, 779
BstDSI CCRYGG 3 cut(s) 108, 421, 511
BstF5I GGATG 1 cut(s) 346
BstFNI CGCG 1 cut(s) 152
BstHHI GCGC 2 cut(s) 125, 152
BstKTI GATC 6 cut(s) 166, 235, 361, 366, 594, 751
BstMAI GTCTC 1 cut(s) 850
BstMBI GATC 6 cut(s) 163, 232, 358, 363, 591, 748
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 1 cut(s) 103
BstNSI RCATGY 1 cut(s) 520
BstSCI CCNGG 2 cut(s) 101, 125
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 1 cut(s) 484
BstV2I GAAGAC 2 cut(s) 31, 846
BstX2I RGATCY 2 cut(s) 358, 591
BstYI RGATCY 2 cut(s) 358, 591
BsuRI GGCC 2 cut(s) 426, 443
BtgI CCRYGG 3 cut(s) 108, 421, 511
BtsCI GGATG 1 cut(s) 346
BtsI GCAGTG 2 cut(s) 150, 710
BtsIMutI CAGTG 4 cut(s) 145, 150, 664, 710
Cac8I GCNNGC 3 cut(s) 518, 802, 868
CfoI GCGC 2 cut(s) 125, 152
Cfr13I GGNCC 2 cut(s) 105, 212
CseI GACGC 1 cut(s) 141
Csp6I GTAC 6 cut(s) 181, 250, 568, 744, 793, 862
CviAII CATG 5 cut(s) 422, 512, 517, 821, 853
CviQI GTAC 6 cut(s) 181, 250, 568, 744, 793, 862
DdeI CTNAG 5 cut(s) 284, 320, 562, 764, 779
DpnI GATC 6 cut(s) 165, 234, 360, 365, 593, 750
DpnII GATC 6 cut(s) 163, 232, 358, 363, 591, 748
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 776
Eco130I CCWWGG 3 cut(s) 421, 511, 530
Eco147I AGGCCT 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 778
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 2 cut(s) 105, 212
Eco53kI GAGCTC 1 cut(s) 776
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 1 cut(s) 776
EcoRI GAATTC 2 cut(s) 75, 625
EcoRII CCWGG 1 cut(s) 101
EcoRV GATATC 1 cut(s) 346
EcoT14I CCWWGG 3 cut(s) 421, 511, 530
EcoT38I GRGCYC 1 cut(s) 778
ErhI CCWWGG 3 cut(s) 421, 511, 530
FaeI CATG 5 cut(s) 425, 515, 520, 824, 856
FaqI GGGAC 1 cut(s) 512
FatI CATG 5 cut(s) 421, 511, 516, 820, 852
Fnu4HI GCNGC 1 cut(s) 473
FokI GGATG 1 cut(s) 353
FriOI GRGCYC 1 cut(s) 778
Fsp4HI GCNGC 1 cut(s) 473
FspBI CTAG 2 cut(s) 723, 871
GlaI GCGC 2 cut(s) 124, 151
GluI GCNGC 1 cut(s) 473
GsuI CTGGAG 1 cut(s) 640
HaeIII GGCC 2 cut(s) 426, 443
HapII CCGG 3 cut(s) 23, 126, 200
HgaI GACGC 1 cut(s) 141
HhaI GCGC 2 cut(s) 125, 152
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 5 cut(s) 425, 515, 520, 824, 856
Hin6I GCGC 2 cut(s) 123, 150
HinP1I GCGC 2 cut(s) 123, 150
HincII GTYRAC 2 cut(s) 454, 703
HindII GTYRAC 2 cut(s) 454, 703
HinfI GANTC 4 cut(s) 274, 280, 525, 768
HpaII CCGG 3 cut(s) 23, 126, 200
HphI GGTGA 2 cut(s) 12, 214
Hpy166II GTNNAC 4 cut(s) 108, 454, 676, 703
Hpy188III TCNNGA 2 cut(s) 236, 299
Hpy8I GTNNAC 4 cut(s) 108, 454, 676, 703
Hpy99I CGWCG 2 cut(s) 85, 157
HpyCH4III ACNGT 4 cut(s) 62, 112, 140, 861
HpyCH4IV ACGT 2 cut(s) 415, 456
HpyCH4V TGCA 3 cut(s) 143, 683, 804
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 5 cut(s) 284, 320, 562, 764, 779
HpySE526I ACGT 2 cut(s) 415, 456
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 5 cut(s) 425, 515, 520, 824, 856
HspAI GCGC 2 cut(s) 123, 150
Kzo9I GATC 6 cut(s) 163, 232, 358, 363, 591, 748
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 202, 849
Lsp1109I GCAGC 1 cut(s) 484
LweI GCATC 1 cut(s) 610
MaeI CTAG 2 cut(s) 723, 871
MaeII ACGT 2 cut(s) 415, 456
MaeIII GTNAC 1 cut(s) 18
MalI GATC 6 cut(s) 165, 234, 360, 365, 593, 750
MboI GATC 6 cut(s) 163, 232, 358, 363, 591, 748
MboII GAAGA 4 cut(s) 31, 242, 601, 851
MflI RGATCY 2 cut(s) 358, 591
MhlI GDGCHC 1 cut(s) 778
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 6 cut(s) 75, 158, 169, 388, 625, 639
MluNI TGGCCA 1 cut(s) 426
MlyI GAGTC 2 cut(s) 274, 534
MmeI TCCRAC 2 cut(s) 104, 135
MnlI CCTC 5 cut(s) 279, 283, 335, 571, 642
Mox20I TGGCCA 1 cut(s) 426
MscI TGGCCA 1 cut(s) 426
MseI TTAA 3 cut(s) 132, 173, 755
Msp20I TGGCCA 1 cut(s) 426
MspI CCGG 3 cut(s) 23, 126, 200
MspR9I CCNGG 2 cut(s) 103, 127
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 149
NciI CCSGG 1 cut(s) 127
NcoI CCATGG 2 cut(s) 421, 511
NdeII GATC 6 cut(s) 163, 232, 358, 363, 591, 748
NlaIII CATG 5 cut(s) 425, 515, 520, 824, 856
NlaIV GGNNCC 3 cut(s) 198, 213, 559
NmeAIII GCCGAG 2 cut(s) 173, 246
NmuCI GTSAC 1 cut(s) 18
NspI RCATGY 1 cut(s) 520
NspV TTCGAA 1 cut(s) 73
PaeI GCATGC 1 cut(s) 520
PceI AGGCCT 1 cut(s) 443
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 2 cut(s) 274, 768
Pfl23II CGTACG 1 cut(s) 180
PkrI GCNGC 1 cut(s) 474
PleI GAGTC 2 cut(s) 274, 533
PpsI GAGTC 2 cut(s) 274, 533
PsiI TTATAA 1 cut(s) 491
Psp124BI GAGCTC 1 cut(s) 778
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspLI CGTACG 1 cut(s) 180
PspN4I GGNNCC 3 cut(s) 198, 213, 559
PspPI GGNCC 2 cut(s) 105, 212
PsrI GAACNNNNNNTAC 2 cut(s) 173, 205
PsuI RGATCY 2 cut(s) 358, 591
RsaI GTAC 6 cut(s) 182, 251, 569, 745, 794, 863
RsaNI GTAC 6 cut(s) 181, 250, 568, 744, 793, 862
SacI GAGCTC 1 cut(s) 778
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 132, 173, 755
SatI GCNGC 1 cut(s) 473
Sau3AI GATC 6 cut(s) 163, 232, 358, 363, 591, 748
Sau96I GGNCC 2 cut(s) 105, 212
ScaI AGTACT 1 cut(s) 251
SchI GAGTC 2 cut(s) 274, 534
ScrFI CCNGG 2 cut(s) 103, 127
SduI GDGCHC 1 cut(s) 778
SfaNI GCATC 1 cut(s) 610
SfuI TTCGAA 1 cut(s) 73
SinI GGWCC 2 cut(s) 105, 212
SphI GCATGC 1 cut(s) 520
Sse9I AATT 6 cut(s) 75, 158, 169, 388, 625, 639
SseBI AGGCCT 1 cut(s) 443
SsiI CCGC 1 cut(s) 731
SspMI CTAG 2 cut(s) 723, 871
SstI GAGCTC 1 cut(s) 778
StuI AGGCCT 1 cut(s) 443
StyD4I CCNGG 2 cut(s) 101, 125
StyI CCWWGG 3 cut(s) 421, 511, 530
TaaI ACNGT 4 cut(s) 62, 112, 140, 861
TaiI ACGT 2 cut(s) 418, 459
TaqI TCGA 5 cut(s) 73, 95, 327, 547, 648
TasI AATT 6 cut(s) 75, 158, 169, 388, 625, 639
TatI WGTACW 4 cut(s) 249, 567, 743, 792
TfiI GAWTC 2 cut(s) 274, 768
Tru1I TTAA 3 cut(s) 132, 173, 755
Tru9I TTAA 3 cut(s) 132, 173, 755
TscAI CASTG 4 cut(s) 145, 150, 664, 717
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 1 cut(s) 472
Tsp45I GTSAC 1 cut(s) 18
TspGWI ACGGA 1 cut(s) 6
TspRI CASTG 4 cut(s) 145, 150, 664, 717
VpaK11BI GGWCC 2 cut(s) 105, 212
XapI RAATTY 3 cut(s) 75, 169, 625
XceI RCATGY 1 cut(s) 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 528
XspI CTAG 2 cut(s) 723, 871
ZraI GACGTC 1 cut(s) 457
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.