Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008684

Physical Location & Seq

Genomic Coordinates Forward (+)

18647327 .. 18654031

Gene Structure 6705 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1079200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3999 bp

ATGGGCGAACTTGAGGGGTGGGCAGAGCCACCAAGTGGGCTGTTGCCGAATGGCCTATTGCCCAATGAAGCTGCCTCGGTGATGCGAGTGCTCGACTCAGAGCGATGGTTGAAGGCGGAGGAGAGAACTGCAGAGCTTATTGCCTGCATTCAGCCCAATTCACCATCCGAAGAGCGCCGAAATGCTGTTGCCGATTATGTGCAGCGGCTCATCATGAAGTGCTTCCCCTGCCAGGTGTTCACTTTTGGGTCTGTACCCCTCAAGACTTATTTGCCTGATGGAGATATTGACCTGACAGCCTTCAGTAAGAGTCCAAATATGAAGGACACATGGGCCCATCAAGTTCGTGATATGCTTCAGAATGAGGAGAAGAACGAGAATGCTGAATTCCGCGTGAAAGAGGTTCAGTACATTCAGGCAGAAGTTAAGATAATCAAGTGCCTTGTGGAAAACATTGTTGTAGACATATCATTCAATCAGCTCGGGGGCTTGTGCACCCTTTGTTTCCTTGAGGAGGTTGATCATTTGATAAATCAGAATCATTTGTTCAAGCGCAGTATCATACTGATAAAGGCCTGGTGTTATTATGAGAGCCGAATACTGGGCGCTCATCATGGGCTTATCTCAACTTACGCATTGGAAACTTTGGTTTTGTACATATTTCACGTTTTCAACAACTCTTTTTCTGGACCACTTGAGGTCCTGTATCGTTTTCTGGAGTTTTTCAGTAAATTTGACTGGGATAATTTTTGTGTTAGCCTATGGGGTCCTGTACCCATTGGTTCACTTCCAGATGTATCTGCGGAACCTCCTCGAAAAGATGGTGGAGAGTTACTGCTGAGCAAATTGTTTCTTGATGCCTGTAGCTCAGTGTATGCCGTCTTCCCTGGTGGTCAAGAAAATCAGGGCCAACCTTTTGTTTCCAAACATTTTAACGTTATTGACCCTTTGCGAATTAACAACAACCTGGGACGTAGTGTTAGTAAAGGTAATTTCTTTAGGATACGCAGTGCATTTGCGTTTGGGGCTAAAAGGCTGGCCAGATTGCTGGATTGTGCCATAGAGGATCTATTTTCCGAAGTAAATCAGTTTTTTCTAAACACTTGGGACAGACATGGGAGTGGTCATCGACCTGATGCACCGCGAAATGATTTGAGGCGCTTGAGACCTTTAAATCCAGACCAATTACATGGATCGGAGAATCTTAGGAATATTTCAAGCAACCGAAAAAAGGAAAGTTCCTCTGGTCGGGATACTCAAGGTGAGAGAATGCATGGCTTCATTAATGTTCCCTCCCAGCATGCTAGTCATCGTTTAGAAAGCACCTCCGGTAACGGTAGTGTATCTACAGTAACTCATCCTCAAACTCAAAGGATTCATGGGAACACAAACCTCACTAGATCCTCTGATATGATTAGAAAGGAAACGAATTCCGACCTGGGTGCACACATTGATAAAGGTCAGAGAAGTGCTAAACCTGATAACTGGGTGAATGACTTACAAGGGAGGTTTCTTTTTGCAAGGACACGCTCTAGTCCTGAGCTTACTGATACATATGGTGAAGTTTCCACTCAAAGTAGGGGCAAAGCCCCAGAGAGTGGGAAAGGTCAAACTTCAGCAAGGTTGGATAATAGCAGGAGGAAAAACTTGGATTCTGATAGTATGGCAAGCCACAGAAATAGATCAACTGATGATCCTTCATCTGCTAGATCCATCTCATCCCGCCAAAGTCTTGATGCTGCCGTTGATTCAAATAACTACCTTGATGAGTCAGGCATGAGTGTCGTTGCTGACAATTATGCTTCTGTTTTGGGGACACAAGGGATGCACCAAGAGGAGCAAGATCTTGTTAACATGATGGCATCTTCTGCAGCTCATGGTTTTAATGGGCAGGTTCATCTTCCACTCAATTTGGCTTCCGGTCACCTACCGCTTCCGATCCCACCTGCCATTCTTGCTTCTATGGGATATGCTCAGAGAAATATGGGTGGAATGGTTCCTACAAATTTCCCCTTGATGGAGACTCCTTGGGGCACAAATATGCAATTTCCCCAAGGTGTTGTTCCGCCGTCACTGGCCCCTTATTTCCCTGGCATGGGGTTGACCTCAAATCCTGAAGATTCAGTGGAACCTGGTAATGAAAATTTTGGATCTGTGGAAATGAACTCAGGTGAAACGGAGCATGATTTCTGGCATCAGCAAGAGAGGGGATCTACTGGTGGGTTTGATCTTGATAATGGAAGTTATGAAATGCTTCAGGAATTTGATAAGCAACAGTCAACCTCAGCTGGTTATAACTTTCATCCATCATCTCGCGTAGGCTCCTCCGGAAATTCTGTGCGAGCTCAACTGAAGTCTACCAAAGAAAATCATGAGTCAACAAGAGGGGAAGAGCATGTGAATAATTTTCAGTACCAAGATAAAAGAGGTAATGAGGTTTACTTTGATGATCGAAGTGTGAGTTCTAGATCTGCCACGCATGCAAGTTCAGTGAGAAGTAAAACCTCCTCTGAGAGTTCTTGGGAAGGATCATCAGCAAAGGTCTCAAAGTCAACAAGGGAAAAACGTGGTAGGCGAAGTGCTCTTTCAGCAGTGCCATCTACTGCATATGGGAAAGGTAAGAATGTGTCCGAACATTCGTCTACCCAGGCAGATGATGACAACAGAGATTGGAATTCATCACCTACAGCTTTAGGTGCTGAAATGGTAGAAAGAAGCACAGGACCTCAACCTGTTGCGCCTTTCCATTTTCCAGGGCATCAAATGGCCGGTTTTGAACAAACTCAGACGAGTGGATCAGATTCAATGGTACCTTTTGCTCCATTGCTCTTAGGTCCTGGCTCACGACAGAAAGCTACAAATGATTCTGGAATGTTTACATTTTATCCTACGGGGCCTCCGGTTCCATTTGTTACAATGCTTCCTTTTAATTATTTCTCGTCAGAAACGGGAACTTCAGATGTATCAACAAGCCAATTTAGCAGAGAAGAGGGACCAGATAATAGTGATTCTGGTCAAAATTTTGATTCATCTGAGGGAGCTGATCAGCCTATTGAGCCATCAGAGCATAAGTCTGACATTCTTCACAGCGACTTCGCTAGCCATTGGCAGAATTTGCAATACGGACGGTTTTGCCAAACTTCTCGGCACCCTTCACCTGTGGTTTATCCTTCACCAGTTATGATGCCCCCTGGTTACTTACAAGGAAGATTTCCTTGGGATGGTCCTGGGAGACCTCTTTCAGCCAACATGAATCTCTTCACTCAGCTTATGGGTTATGGACCTAGAATGGTTCCTGTTGCTCCTCTTCAGTCTGTTCCTAACAGGCCTGCCGGTGTTTACCAACGCTATGCAGAGGAGATTCCAAGATATCGTGCTGGGACTGGGACATACCTGCCAAATCCCAAGGTTTCTGTACGGGACCGGCCTTCAAATACGAGAAGGGGGAATTACAATTATGATAGAAATGACCACCATGGTGACAGAGAAGGGAACTGGAATGCCAATTCAAAGTCACGAGCTTCTGGGCGCAACCACAGTCGCGGTCAAGCTGAGAAGCCAAATTCCAGAGTAAACCGTTTGGCAGCCAGTGAGAGCCGAGCTGAAAGGTCATGGAGTTCACATAGGCAGGACTCATTCCAGTCATATCAATCTCAAAATGGTCCTATCCGCACAAACACTACACAGAGTGGTTCGACCAATGTTGCATATGGCATGTATCCGGTACCAACCATGAACCCCAGTGGGGTATCTTCGAATGGACCTTCCATGCCACCTGTTGTCATGCTATATCATTATGATCATAATGCTGGCTACGGGCCACCTGCAGAACAACTTGAGTTTGGTTCTCTTGGACCGGTGGGATTCTCAGGTTTGAATGAAGCACAGCTAAATGAAGGGAGCCAGATGAGTGGGGTATTTGAGGAACAAAGGTTTCATGGTGGCTCTACTCAACGATCTTCACCTGATCAGCCTTCTTCACCCCACCTCCAAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004652 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006396 GO:0006399 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016074 GO:0016075 GO:0016077 GO:0016078 GO:0016740 GO:0016772 GO:0016779 GO:0019439 GO:0031123 GO:0031499 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0043021 GO:0043023 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043629 GO:0043630 GO:0043631 GO:0043632 GO:0043633 GO:0043634 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0046483 GO:0046700 GO:0070013 GO:0070566 GO:0071025 GO:0071027 GO:0071029 GO:0071035 GO:0071046 GO:0071704 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1990817

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTP_transf_2

Protein Analysis

1333

Amino Acids

147.42

Weight (kDa)

6.18

Isoelectric Point (pI)

51.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTPAP-like_central	PF22600	44 - 164	2.6e-10	Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1	PF26180	177 - 369	4.2e-92	PAP/OAS1 substrate-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61690 AT3G61690 AT3G61690
fragaria_vesca	FvH4_7g16250 FvH4_7g16250 FvH4_7g16250 FvH4_7g16250
malus_domestica	MD01G1079200.v1.1 MD07G1148100.v1.1
prunus_persica	Prupe.2G186200_v2.0.a1 Prupe.2G186200_v2.0.a1
pyrus_communis	pycom01g10750 pycom07g14710
rosa_chinensis	RchiOBHm_Chr1g0359271
rosa_laevigata	RLG00000027887
rosa_multiflora	Rmu_ssc0000135.1_g000035
rosa_roxburghii	Rroxscaffold_4G00297170
rosa_rugosa	Rorug01G0269000
rosa_samantha	Rh1AG283000 Rh1BG249200 Rh1CG267000 Rh1DG278100
rosa_wichuraiana	Rw1G025120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2295
AarI	CACCTGC	2 cut(s)	1954, 3832
Acc36I	ACCTGC	4 cut(s)	1882, 1954, 3402, 3832
Acc65I	GGTACC	2 cut(s)	2809, 3724
AccB1I	GGYRCC	3 cut(s)	2809, 3147, 3724
AccB7I	CCANNNNNTGG	2 cut(s)	35, 1598
AccI	GTMKAC	3 cut(s)	462, 2357, 2642
AccII	CGCG	4 cut(s)	393, 1144, 2316, 3543
AccIII	TCCGGA	1 cut(s)	2327
AclI	AACGTT	1 cut(s)	936
AcoI	YGGCCR	2 cut(s)	1038, 2766
AcuI	CTGAAG	8 cut(s)	286, 341, 1599, 2136, 2240, 2372, 2940, 3293
AdeI	CACNNNGTG	2 cut(s)	35, 3689
AfaI	GTAC	8 cut(s)	255, 410, 656, 774, 2414, 2811, 3417, 3726
AgeI	ACCGGT	1 cut(s)	3856
AjuI	GAANNNNNNNTTGG	4 cut(s)	2046, 2078, 3199, 3231
AleI	CACNNNNGTG	1 cut(s)	3477
Alw21I	GWGCWC	5 cut(s)	93, 497, 1447, 2347, 2584
Alw26I	GTCTC	4 cut(s)	1159, 2015, 2548, 3226
Alw44I	GTGCAC	2 cut(s)	493, 1443
Ama87I	CYCGRG	1 cut(s)	482
Aor13HI	TCCGGA	1 cut(s)	2327
ApaI	GGGCCC	1 cut(s)	337
ApaLI	GTGCAC	2 cut(s)	493, 1443
ApeKI	GCWGC	5 cut(s)	71, 202, 1739, 1871, 3584
AseI	ATTAAT	1 cut(s)	1284
AsiGI	ACCGGT	1 cut(s)	3856
Asp700I	GAANNNNTTC	3 cut(s)	221, 2253, 3257
Asp718I	GGTACC	2 cut(s)	2809, 3724
AspLEI	GCGC	6 cut(s)	177, 555, 608, 1161, 2740, 3531
AsuII	TTCGAA	1 cut(s)	3755
AsuNHI	GCTAGC	1 cut(s)	3098
AvaI	CYCGRG	1 cut(s)	482
BaeGI	GKGCMC	4 cut(s)	337, 497, 1447, 2036
BalI	TGGCCA	1 cut(s)	1040
BanI	GGYRCC	3 cut(s)	2809, 3147, 3724
BanII	GRGCYC	2 cut(s)	337, 2347
BarI	GAAGNNNNNNTAC	2 cut(s)	866, 898
BauI	CACGAG	1 cut(s)	3516
BbsI	GAAGAC	1 cut(s)	874
Bbv12I	GWGCWC	5 cut(s)	93, 497, 1447, 2347, 2584
BbvCI	CCTCAGC	1 cut(s)	2284
BbvI	GCAGC	5 cut(s)	58, 214, 1726, 1883, 3596
BceAI	ACGGC	3 cut(s)	863, 1727, 2053
BcgI	CGANNNNNNTGC	4 cut(s)	1424, 1458, 1765, 1799
BciVI	GTATCC	3 cut(s)	996, 1246, 3729
BclI	TGATCA	4 cut(s)	520, 3043, 3799, 3967
BcoDI	GTCTC	4 cut(s)	1159, 2015, 2548, 3226
BfaI	CTAG	7 cut(s)	1305, 1398, 1533, 1707, 2466, 3099, 3285
BfmI	CTRYAG	6 cut(s)	129, 862, 1347, 1869, 2685, 3825
BfoI	RGCGCY	3 cut(s)	178, 609, 1162
BfuAI	ACCTGC	4 cut(s)	1882, 1954, 3402, 3832
BfuI	GTATCC	3 cut(s)	996, 1246, 3729
BglII	AGATCT	2 cut(s)	1843, 2468
BisI	GCNGC	6 cut(s)	72, 203, 206, 1740, 1872, 3585
BlpI	GCTNAGC	1 cut(s)	839
BlsI	GCNGC	6 cut(s)	73, 204, 207, 1741, 1873, 3586
BmeT110I	CYCGRG	1 cut(s)	482
BmrI	ACTGGG	5 cut(s)	611, 748, 1495, 3393, 3735
BmsI	GCATC	9 cut(s)	72, 847, 1126, 1726, 1815, 1871, 2203, 2767, 3174
BmtI	GCTAGC	1 cut(s)	3102
BmuI	ACTGGG	5 cut(s)	611, 748, 1495, 3393, 3735
BpiI	GAAGAC	1 cut(s)	874
BpmI	CTGGAG	1 cut(s)	737
Bpu10I	CCTNAGC	2 cut(s)	1539, 2284
Bpu1102I	GCTNAGC	1 cut(s)	839
Bpu14I	TTCGAA	1 cut(s)	3755
BpuEI	CTTGAG	7 cut(s)	32, 245, 530, 716, 1183, 1242, 3857
BsaI	GGTCTC	3 cut(s)	1159, 2548, 3226
BsaWI	WCCGGW	6 cut(s)	1328, 1919, 2327, 2899, 3721, 3856
BsaXI	ACNNNNNCTCC	3 cut(s)	2881, 2911, 3972
Bse118I	RCCGGY	4 cut(s)	2768, 3332, 3423, 3856
Bse3DI	GCAATG	1 cut(s)	2822
BseAI	TCCGGA	1 cut(s)	2327
BseGI	GGATG	6 cut(s)	164, 1357, 1718, 1830, 2302, 3226
BseMI	GCAATG	1 cut(s)	2822
BseRI	GAGGAG	9 cut(s)	134, 380, 527, 801, 1850, 2314, 2497, 3294, 3371
BseSI	GKGCMC	4 cut(s)	337, 497, 1447, 2036
BseXI	GCAGC	5 cut(s)	58, 214, 1726, 1883, 3596
BseYI	CCCAGC	2 cut(s)	1296, 3377
BsgI	GTGCAG	1 cut(s)	221
Bsh1236I	CGCG	4 cut(s)	393, 1144, 2316, 3543
BshNI	GGYRCC	3 cut(s)	2809, 3147, 3724
BshTI	ACCGGT	1 cut(s)	3856
BsiHKAI	GWGCWC	5 cut(s)	93, 497, 1447, 2347, 2584
BsiHKCI	CYCGRG	1 cut(s)	482
BsiSI	CCGG	9 cut(s)	1329, 1920, 2328, 2769, 2900, 3333, 3424, 3722, 3857
BslFI	GGGAC	7 cut(s)	984, 1121, 1828, 3006, 3394, 3400, 3434
BsmAI	GTCTC	4 cut(s)	1159, 2015, 2548, 3226
BsmFI	GGGAC	7 cut(s)	984, 1121, 1828, 3006, 3394, 3400, 3434
BsmI	GAATGC	4 cut(s)	147, 385, 1275, 3505
Bso31I	GGTCTC	3 cut(s)	1159, 2548, 3226
BsoBI	CYCGRG	1 cut(s)	482
Bsp119I	TTCGAA	1 cut(s)	3755
Bsp120I	GGGCCC	1 cut(s)	333
Bsp1286I	GDGCHC	7 cut(s)	93, 337, 497, 1447, 2036, 2347, 2584
Bsp13I	TCCGGA	1 cut(s)	2327
Bsp1407I	TGTACA	1 cut(s)	654
Bsp1720I	GCTNAGC	1 cut(s)	839
Bsp19I	CCATGG	1 cut(s)	3475
BspEI	TCCGGA	1 cut(s)	2327
BspFNI	CGCG	4 cut(s)	393, 1144, 2316, 3543
BspHI	TCATGA	2 cut(s)	213, 2371
BspMAI	CTGCAG	3 cut(s)	133, 1873, 3829
BspMI	ACCTGC	4 cut(s)	1882, 1954, 3402, 3832
BspOI	GCTAGC	1 cut(s)	3102
BspQI	GCTCTTC	2 cut(s)	165, 2385
BspT104I	TTCGAA	1 cut(s)	3755
BspT107I	GGYRCC	3 cut(s)	2809, 3147, 3724
BspTNI	GGTCTC	3 cut(s)	1159, 2548, 3226
BsrDI	GCAATG	1 cut(s)	2822
BsrFI	RCCGGY	4 cut(s)	2768, 3332, 3423, 3856
BsrGI	TGTACA	1 cut(s)	654
BssAI	RCCGGY	4 cut(s)	2768, 3332, 3423, 3856
BssSI	CACGAG	1 cut(s)	3516
BssT1I	CCWWGG	5 cut(s)	2027, 2053, 3215, 3405, 3475
Bst2BI	CACGAG	1 cut(s)	3516
Bst4CI	ACNGT	6 cut(s)	1337, 1351, 2277, 3129, 3539, 3578
Bst6I	CTCTTC	5 cut(s)	165, 2385, 2982, 3263, 3312
BstAPI	GCANNNNNTGC	2 cut(s)	1868, 3115
BstAUI	TGTACA	1 cut(s)	654
BstBI	TTCGAA	1 cut(s)	3755
BstC8I	GCNNGC	9 cut(s)	145, 1038, 1302, 1669, 2343, 2481, 3100, 3330, 3811
BstDSI	CCRYGG	1 cut(s)	3475
BstEII	GGTNACC	1 cut(s)	1922
BstENI	CCTNNNNNAGG	1 cut(s)	512
BstF5I	GGATG	6 cut(s)	164, 1357, 1718, 1830, 2302, 3226
BstFNI	CGCG	4 cut(s)	393, 1144, 2316, 3543
BstH2I	RGCGCY	3 cut(s)	178, 609, 1162
BstHHI	GCGC	6 cut(s)	177, 555, 608, 1161, 2740, 3531
BstMAI	GTCTC	4 cut(s)	1159, 2015, 2548, 3226
BstNSI	RCATGY	4 cut(s)	1304, 2399, 2483, 3718
BstPI	GGTNACC	1 cut(s)	1922
BstSFI	CTRYAG	6 cut(s)	129, 862, 1347, 1869, 2685, 3825
BstSLI	GKGCMC	4 cut(s)	337, 497, 1447, 2036
BstUI	CGCG	4 cut(s)	393, 1144, 2316, 3543
BstV1I	GCAGC	5 cut(s)	58, 214, 1726, 1883, 3596
BstV2I	GAAGAC	1 cut(s)	874
BstX2I	RGATCY	7 cut(s)	1066, 1400, 1709, 1843, 2150, 2210, 2468
BstXI	CCANNNNNNTGG	3 cut(s)	1048, 1190, 3911
BstYI	RGATCY	7 cut(s)	1066, 1400, 1709, 1843, 2150, 2210, 2468
BsuI	GTATCC	3 cut(s)	996, 1246, 3729
BtgI	CCRYGG	1 cut(s)	3475
BtgZI	GCGATG	1 cut(s)	118
BtsCI	GGATG	6 cut(s)	164, 1357, 1718, 1830, 2302, 3226
BtsI	GCAGTG	2 cut(s)	1015, 2598
BtsIMutI	CAGTG	8 cut(s)	876, 1015, 2072, 2130, 2496, 2598, 3595, 3748
BveI	ACCTGC	4 cut(s)	1882, 1954, 3402, 3832
Cac8I	GCNNGC	9 cut(s)	145, 1038, 1302, 1669, 2343, 2481, 3100, 3330, 3811
CciI	TCATGA	2 cut(s)	213, 2371
CfoI	GCGC	6 cut(s)	177, 555, 608, 1161, 2740, 3531
Cfr10I	RCCGGY	4 cut(s)	2768, 3332, 3423, 3856
CsiI	ACCWGGT	1 cut(s)	2131
Csp6I	GTAC	8 cut(s)	254, 409, 655, 773, 2413, 2810, 3416, 3725
CspAI	ACCGGT	1 cut(s)	3856
CspCI	CAANNNNNGTGG	2 cut(s)	1893, 1928
CviQI	GTAC	8 cut(s)	254, 409, 655, 773, 2413, 2810, 3416, 3725
DraI	TTTAAA	1 cut(s)	1173
DraIII	CACNNNGTG	2 cut(s)	35, 3689
EaeI	YGGCCR	2 cut(s)	1038, 2766
Eam1104I	CTCTTC	5 cut(s)	165, 2385, 2982, 3263, 3312
EarI	CTCTTC	5 cut(s)	165, 2385, 2982, 3263, 3312
EciI	GGCGGA	2 cut(s)	131, 2055
Ecl136II	GAGCTC	1 cut(s)	2345
Eco130I	CCWWGG	5 cut(s)	2027, 2053, 3215, 3405, 3475
Eco147I	AGGCCT	2 cut(s)	575, 3328
Eco24I	GRGCYC	2 cut(s)	337, 2347
Eco31I	GGTCTC	3 cut(s)	1159, 2548, 3226
Eco32I	GATATC	1 cut(s)	3371
Eco53kI	GAGCTC	1 cut(s)	2345
Eco57I	CTGAAG	8 cut(s)	286, 341, 1599, 2136, 2240, 2372, 2940, 3293
Eco88I	CYCGRG	1 cut(s)	482
Eco91I	GGTNACC	1 cut(s)	1922
EcoICRI	GAGCTC	1 cut(s)	2345
EcoNI	CCTNNNNNAGG	1 cut(s)	512
EcoO109I	RGGNCCY	5 cut(s)	700, 767, 2723, 2834, 2894
EcoO65I	GGTNACC	1 cut(s)	1922
EcoRI	GAATTC	3 cut(s)	386, 1429, 2674
EcoRV	GATATC	1 cut(s)	3371
EcoT14I	CCWWGG	5 cut(s)	2027, 2053, 3215, 3405, 3475
EcoT22I	ATGCAT	1 cut(s)	1275
EcoT38I	GRGCYC	2 cut(s)	337, 2347
ErhI	CCWWGG	5 cut(s)	2027, 2053, 3215, 3405, 3475
FalI	AAGNNNNNCTT	2 cut(s)	3199, 3231
FaqI	GGGAC	7 cut(s)	984, 1121, 1828, 3006, 3394, 3400, 3434
FauI	CCCGC	1 cut(s)	1730
FauNDI	CATATG	3 cut(s)	1555, 2608, 3709
FbaI	TGATCA	4 cut(s)	520, 3043, 3799, 3967
FblI	GTMKAC	3 cut(s)	462, 2357, 2642
Fnu4HI	GCNGC	6 cut(s)	72, 203, 206, 1740, 1872, 3585
FokI	GGATG	6 cut(s)	151, 1344, 1705, 1837, 2289, 3233
FriOI	GRGCYC	2 cut(s)	337, 2347
Fsp4HI	GCNGC	6 cut(s)	72, 203, 206, 1740, 1872, 3585
FspBI	CTAG	7 cut(s)	1305, 1398, 1533, 1707, 2466, 3099, 3285
GlaI	GCGC	6 cut(s)	176, 554, 607, 1160, 2739, 3530
GluI	GCNGC	6 cut(s)	72, 203, 206, 1740, 1872, 3585
GsaI	CCCAGC	2 cut(s)	1300, 3381
GsuI	CTGGAG	1 cut(s)	737
HaeII	RGCGCY	3 cut(s)	178, 609, 1162
HapII	CCGG	9 cut(s)	1329, 1920, 2328, 2769, 2900, 3333, 3424, 3722, 3857
HhaI	GCGC	6 cut(s)	177, 555, 608, 1161, 2740, 3531
Hin6I	GCGC	6 cut(s)	175, 553, 606, 1159, 2738, 3529
HinP1I	GCGC	6 cut(s)	175, 553, 606, 1159, 2738, 3529
HincII	GTYRAC	5 cut(s)	1852, 2103, 2280, 2379, 2553
HindII	GTYRAC	5 cut(s)	1852, 2103, 2280, 2379, 2553
HpaI	GTTAAC	1 cut(s)	1852
HpaII	CCGG	9 cut(s)	1329, 1920, 2328, 2769, 2900, 3333, 3424, 3722, 3857
HpyCH4III	ACNGT	6 cut(s)	1337, 1351, 2277, 3129, 3539, 3578
HpyCH4IV	ACGT	4 cut(s)	666, 936, 973, 2566
HpySE526I	ACGT	4 cut(s)	666, 936, 973, 2566
HspAI	GCGC	6 cut(s)	175, 553, 606, 1159, 2738, 3529
Kpn2I	TCCGGA	1 cut(s)	2327
KpnI	GGTACC	2 cut(s)	2813, 3728
Ksp22I	TGATCA	4 cut(s)	520, 3043, 3799, 3967
KspAI	GTTAAC	1 cut(s)	1852
LguI	GCTCTTC	2 cut(s)	165, 2385
LmnI	GCTCC	7 cut(s)	1837, 2179, 2327, 2824, 3038, 3307, 3900
Lsp1109I	GCAGC	5 cut(s)	58, 214, 1726, 1883, 3596
LweI	GCATC	9 cut(s)	72, 847, 1126, 1726, 1815, 1871, 2203, 2767, 3174
MabI	ACCWGGT	1 cut(s)	2131
MaeI	CTAG	7 cut(s)	1305, 1398, 1533, 1707, 2466, 3099, 3285
MaeII	ACGT	4 cut(s)	666, 936, 973, 2566
MaeIII	GTNAC	9 cut(s)	831, 1331, 1351, 1922, 2070, 2911, 3194, 3479, 3513
MflI	RGATCY	7 cut(s)	1066, 1400, 1709, 1843, 2150, 2210, 2468
MhlI	GDGCHC	7 cut(s)	93, 337, 497, 1447, 2036, 2347, 2584
MlsI	TGGCCA	1 cut(s)	1040
MluNI	TGGCCA	1 cut(s)	1040
MlyI	GAGTC	6 cut(s)	89, 319, 1778, 2017, 2384, 3626
MmeI	TCCRAC	2 cut(s)	1458, 1605
Mox20I	TGGCCA	1 cut(s)	1040
Mph1103I	ATGCAT	1 cut(s)	1275
MroI	TCCGGA	1 cut(s)	2327
MroXI	GAANNNNTTC	3 cut(s)	221, 2253, 3257
MscI	TGGCCA	1 cut(s)	1040
MseI	TTAA	8 cut(s)	426, 933, 957, 1172, 1284, 1851, 1884, 2928
MslI	CAYNNNNRTG	3 cut(s)	1119, 2039, 3477
Msp20I	TGGCCA	1 cut(s)	1040
MspA1I	CMGCKG	2 cut(s)	205, 2288
MspI	CCGG	9 cut(s)	1329, 1920, 2328, 2769, 2900, 3333, 3424, 3722, 3857
Mva1269I	GAATGC	4 cut(s)	147, 385, 1275, 3505
MvnI	CGCG	4 cut(s)	393, 1144, 2316, 3543
NcoI	CCATGG	1 cut(s)	3475
NdeI	CATATG	3 cut(s)	1555, 2608, 3709
NheI	GCTAGC	1 cut(s)	3098
NmeAIII	GCCGAG	2 cut(s)	3124, 3623
NmuCI	GTSAC	4 cut(s)	1922, 2070, 3479, 3513
NsiI	ATGCAT	1 cut(s)	1275
NspI	RCATGY	4 cut(s)	1304, 2399, 2483, 3718
NspV	TTCGAA	1 cut(s)	3755
OliI	CACNNNNGTG	1 cut(s)	3477
PaeI	GCATGC	2 cut(s)	1304, 2483
PagI	TCATGA	2 cut(s)	213, 2371
PaqCI	CACCTGC	2 cut(s)	1954, 3832
PceI	AGGCCT	2 cut(s)	575, 3328
PciSI	GCTCTTC	2 cut(s)	165, 2385
PcsI	WCGNNNNNNNCGW	1 cut(s)	2572
PctI	GAATGC	4 cut(s)	147, 385, 1275, 3505
PdmI	GAANNNNTTC	3 cut(s)	221, 2253, 3257
PflMI	CCANNNNNTGG	2 cut(s)	35, 1598
PinAI	ACCGGT	1 cut(s)	3856
PkrI	GCNGC	6 cut(s)	73, 204, 207, 1741, 1873, 3586
PleI	GAGTC	6 cut(s)	89, 318, 1777, 2017, 2383, 3626
PpsI	GAGTC	6 cut(s)	89, 318, 1777, 2017, 2383, 3626
PpuMI	RGGWCCY	4 cut(s)	700, 767, 2723, 2834
PshBI	ATTAAT	1 cut(s)	1284
PsiI	TTATAA	1 cut(s)	2295
Psp124BI	GAGCTC	1 cut(s)	2347
Psp1406I	AACGTT	1 cut(s)	936
Psp5II	RGGWCCY	4 cut(s)	700, 767, 2723, 2834
PspEI	GGTNACC	1 cut(s)	1922
PspFI	CCCAGC	2 cut(s)	1296, 3377
PspOMI	GGGCCC	1 cut(s)	333
PspPPI	RGGWCCY	4 cut(s)	700, 767, 2723, 2834
PstI	CTGCAG	3 cut(s)	133, 1873, 3829
PsuI	RGATCY	7 cut(s)	1066, 1400, 1709, 1843, 2150, 2210, 2468
PvuII	CAGCTG	1 cut(s)	2288
RsaI	GTAC	8 cut(s)	255, 410, 656, 774, 2414, 2811, 3417, 3726
RsaNI	GTAC	8 cut(s)	254, 409, 655, 773, 2413, 2810, 3416, 3725
RseI	CAYNNNNRTG	3 cut(s)	1119, 2039, 3477
SacI	GAGCTC	1 cut(s)	2347
SapI	GCTCTTC	2 cut(s)	165, 2385
SaqAI	TTAA	8 cut(s)	426, 933, 957, 1172, 1284, 1851, 1884, 2928
SatI	GCNGC	6 cut(s)	72, 203, 206, 1740, 1872, 3585
SchI	GAGTC	6 cut(s)	89, 319, 1778, 2017, 2384, 3626
SduI	GDGCHC	7 cut(s)	93, 337, 497, 1447, 2036, 2347, 2584
SexAI	ACCWGGT	1 cut(s)	2131
SfaNI	GCATC	9 cut(s)	72, 847, 1126, 1726, 1815, 1871, 2203, 2767, 3174
SfcI	CTRYAG	6 cut(s)	129, 862, 1347, 1869, 2685, 3825
SfuI	TTCGAA	1 cut(s)	3755
SmiMI	CAYNNNNRTG	3 cut(s)	1119, 2039, 3477
SmlI	CTYRAG	7 cut(s)	11, 260, 509, 695, 1162, 1257, 3836
SmoI	CTYRAG	7 cut(s)	11, 260, 509, 695, 1162, 1257, 3836
SphI	GCATGC	2 cut(s)	1304, 2483
SseBI	AGGCCT	2 cut(s)	575, 3328
SspI	AATATT	1 cut(s)	1213
SspMI	CTAG	7 cut(s)	1305, 1398, 1533, 1707, 2466, 3099, 3285
SstI	GAGCTC	1 cut(s)	2347
StuI	AGGCCT	2 cut(s)	575, 3328
StyI	CCWWGG	5 cut(s)	2027, 2053, 3215, 3405, 3475
TaaI	ACNGT	6 cut(s)	1337, 1351, 2277, 3129, 3539, 3578
TaiI	ACGT	4 cut(s)	669, 939, 976, 2569
TaqI	TCGA	6 cut(s)	93, 814, 1129, 2452, 3695, 3755
TatI	WGTACW	2 cut(s)	408, 654
TauI	GCSGC	1 cut(s)	208
Tru1I	TTAA	8 cut(s)	426, 933, 957, 1172, 1284, 1851, 1884, 2928
Tru9I	TTAA	8 cut(s)	426, 933, 957, 1172, 1284, 1851, 1884, 2928
TscAI	CASTG	8 cut(s)	876, 1015, 2079, 2130, 2496, 2598, 3595, 3748
TseFI	GTSAC	4 cut(s)	1922, 2070, 3479, 3513
TseI	GCWGC	5 cut(s)	71, 202, 1739, 1871, 3584
Tsp45I	GTSAC	4 cut(s)	1922, 2070, 3479, 3513
TspGWI	ACGGA	2 cut(s)	2192, 3138
TspRI	CASTG	8 cut(s)	876, 1015, 2079, 2130, 2496, 2598, 3595, 3748
Van91I	CCANNNNNTGG	2 cut(s)	35, 1598
VneI	GTGCAC	2 cut(s)	493, 1443
VspI	ATTAAT	1 cut(s)	1284
XagI	CCTNNNNNAGG	1 cut(s)	512
XbaI	TCTAGA	1 cut(s)	2465
XceI	RCATGY	4 cut(s)	1304, 2399, 2483, 3718
XmiI	GTMKAC	3 cut(s)	462, 2357, 2642
XmnI	GAANNNNTTC	3 cut(s)	221, 2253, 3257
XspI	CTAG	7 cut(s)	1305, 1398, 1533, 1707, 2466, 3099, 3285
Zsp2I	ATGCAT	1 cut(s)	1275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.