MD01G1113300.v1.1

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014410
Physical Location & Seq
Reverse (-)
22719673 .. 22722321
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1113300.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGCGGCGCGCCATAGACGCCCACCTCAAGGCGGCGCGTCTCTTTCCCTCCTTATCATACTTCTTTTCTTCCTCCTCACCGTCTACGCCTCGCGTCACCGTCAGTGCAATCCTAACCCTAGCTCATCCCCACGCTCCTTCCATTTCCAAAACGCTGAACCTCCACTCGATCACAATTTCCTCCTGCTCTTCGATTCTCATAAACCACGGCATCGCCTCCAATTTTGGCCTCGCCGTTGTGCGCTGCGTTTCGTCGGTCTCGCATGCCGATACATTGGAGTGGAACGAGCCGGTTTCGTGCTCGGAGGTCGGCGATGGCCACAAAGCCCCTGTGGACGAAGACTCCAAGCCCTCGATTCCTGTCCGAGCATACTTCTTCTCCACCAGTGTGGATTTAAGAAGTTTGGTAGAGCAGAACAGGCTGAATTTCATCCCACCTACATCGCGAATGACTAATTATGTTGTTTTTAAGTTCGGGGAGCTTTCCGATTCCAACGATGGTTTGGGCCTTAGTTTAAGTGGGAGTGACTGTTGCTACATGGTAGTCTTTCAGTATGGCTCCATTGTCTTGTTCAATGTCCGTGAGAAAGATATTGATGGGTATTTGAAAATTGTGGAAAAACACGCATCAGGATCGCTGCCTGAAATGAGAAAGGATGAGTATGAGGTGAGAGAGAACCCCACTTTGCACACATGGATGGAAGGGGGATTAGATTACATAATGTTGCAGTACTTGAATGTTGATGGAATCCGTACTATTGGTAGTGTTCTAGGTCAAAGTATTGCGCTTGATTACTATGTGCGCCAGGTTGATGGAATGGTTGCGGAGTTTACAGACATTAATCGTGAAATGGAAACCACTGGGAAGTTTAAAATGAAGAGGAAGAAACTGTTTCAGTTAGTGGGAAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTCGGACTTTTTGAGAGATCGGATATTGCCTGGAAGGATGCCAAATATGCTCAGATATGGGAATATCTCAGGGATGAATTTGAGTTGACTCAGAGATTTGCAAGTCTTGATTTTAAGTTGAAGTTTGTGGAGCACAATATTCGTTTTCTTCAGGAAATTCTCCAAAACAGGAAGTCAGATTTCTTGGAATGGCTCATCATTGCGTTAATAGGTGCTGAAATTCTTCTCTCCCTTTACGACATTGTCCACAGGTCGGCACTTGCTTCGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

405

Amino Acids

45.56

Weight (kDa)

6.15

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 180 - 350 1.5e-34 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13610
fragaria_vesca FvH4_7g20360
malus_domestica MD01G1113300.v1.1 MD07G1180700.v1.1
prunus_persica Prupe.2G220000_v2.0.a1
pyrus_communis pycom01g14160 pycom07g17400
rosa_chinensis RchiOBHm_Chr1g0364321
rosa_laevigata RLG00000027495
rosa_multiflora Rmu_sc0007470.1_g000002
rosa_roxburghii Rroxscaffold_4G00292430
rosa_rugosa Rorug01G0315400
rosa_samantha Rh1AG323100 Rh1BG285700 Rh1CG302400 Rh1DG317200
rosa_wichuraiana Rw1G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 83
AccII CGCG 4 cut(s) 9, 37, 93, 445
AciI CCGC 3 cut(s) 4, 32, 824
AclWI GGATC 1 cut(s) 640
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 5 cut(s) 424, 913, 1019, 1098, 1161
AcuI CTGAAG 1 cut(s) 1076
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 2 cut(s) 731, 754
AfiI CCNNNNNNNGG 3 cut(s) 28, 31, 1195
AflII CTTAAG 1 cut(s) 938
AgsI TTSAA 4 cut(s) 574, 607, 736, 1063
AjnI CCWGG 2 cut(s) 804, 971
AleI CACNNNNGTG 1 cut(s) 386
AluBI AGCT 3 cut(s) 122, 481, 943
AluI AGCT 3 cut(s) 122, 481, 943
Alw21I GWGCWC 2 cut(s) 302, 1077
Alw26I GTCTC 2 cut(s) 44, 262
AlwI GGATC 1 cut(s) 640
AoxI GGCC 3 cut(s) 226, 316, 505
ApeKI GCWGC 2 cut(s) 243, 637
ApoI RAATTY 5 cut(s) 424, 913, 1019, 1098, 1161
AscI GGCGCGCC 1 cut(s) 7
AseI ATTAAT 1 cut(s) 840
Asp700I GAANNNNTTC 1 cut(s) 1164
AspLEI GCGC 6 cut(s) 9, 11, 37, 243, 787, 804
AspS9I GGNCC 1 cut(s) 505
AsuHPI GGTGA 3 cut(s) 69, 88, 679
BalI TGGCCA 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 345
Bbv12I GWGCWC 2 cut(s) 302, 1077
BbvI GCAGC 2 cut(s) 230, 624
BccI CCATC 6 cut(s) 308, 491, 590, 691, 737, 806
BceAI ACGGC 2 cut(s) 218, 223
BcgI CGANNNNNNTGC 5 cut(s) 615, 649, 1064, 1098, 1188
BciT130I CCWGG 2 cut(s) 806, 973
BcoDI GTCTC 2 cut(s) 44, 262
BfaI CTAG 2 cut(s) 119, 770
BfrI CTTAAG 1 cut(s) 938
BisI GCNGC 4 cut(s) 5, 33, 244, 638
BlsI GCNGC 4 cut(s) 6, 34, 245, 639
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 2 cut(s) 806, 973
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 2 cut(s) 806, 973
BmrI ACTGGG 1 cut(s) 870
BmsI GCATC 3 cut(s) 219, 635, 970
BmuI ACTGGG 1 cut(s) 870
BpiI GAAGAC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 1 cut(s) 205
BsaXI ACNNNNNCTCC 6 cut(s) 326, 356, 362, 392, 514, 544
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 31, 1195
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 2 cut(s) 384, 865
Bse3DI GCAATG 1 cut(s) 1140
BseBI CCWGG 2 cut(s) 806, 973
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 6 cut(s) 124, 429, 661, 702, 985, 1021
BseLI CCNNNNNNNGG 3 cut(s) 28, 31, 1195
BseMI GCAATG 1 cut(s) 1140
BseMII CTCAG 3 cut(s) 1007, 1024, 1046
BseNI ACTGG 2 cut(s) 384, 865
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 1 cut(s) 64
BseXI GCAGC 2 cut(s) 230, 624
Bsh1236I CGCG 4 cut(s) 9, 37, 93, 445
BshFI GGCC 3 cut(s) 228, 318, 507
BsiHKAI GWGCWC 2 cut(s) 302, 1077
BsiSI CCGG 1 cut(s) 290
BslI CCNNNNNNNGG 3 cut(s) 28, 31, 1195
BsmAI GTCTC 2 cut(s) 44, 262
BsmBI CGTCTC 1 cut(s) 44
BsnI GGCC 3 cut(s) 228, 318, 507
Bso31I GGTCTC 1 cut(s) 262
Bsp1286I GDGCHC 2 cut(s) 302, 1077
Bsp143I GATC 3 cut(s) 168, 632, 959
Bsp68I TCGCGA 1 cut(s) 445
BspACI CCGC 3 cut(s) 4, 32, 824
BspANI GGCC 3 cut(s) 228, 318, 507
BspCNI CTCAG 3 cut(s) 1006, 1023, 1045
BspFNI CGCG 4 cut(s) 9, 37, 93, 445
BspLI GGNNCC 1 cut(s) 559
BspPI GGATC 1 cut(s) 640
BspQI GCTCTTC 1 cut(s) 193
BspTI CTTAAG 1 cut(s) 938
BspTNI GGTCTC 1 cut(s) 262
BsrDI GCAATG 1 cut(s) 1140
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 2 cut(s) 384, 865
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 205
BssHII GCGCGC 1 cut(s) 7
BssMI GATC 3 cut(s) 168, 632, 959
BssNI GRCGYC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 806, 973
Bst4CI ACNGT 4 cut(s) 81, 100, 530, 891
Bst6I CTCTTC 2 cut(s) 193, 872
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 938
BstC8I GCNNGC 2 cut(s) 9, 264
BstDEI CTNAG 4 cut(s) 509, 993, 1010, 1032
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 6 cut(s) 124, 429, 661, 702, 985, 1021
BstFNI CGCG 4 cut(s) 9, 37, 93, 445
BstHHI GCGC 6 cut(s) 9, 11, 37, 243, 787, 804
BstKTI GATC 3 cut(s) 171, 635, 962
BstMAI GTCTC 2 cut(s) 44, 262
BstMBI GATC 3 cut(s) 168, 632, 959
BstMWI GCNNNNNNNGC 3 cut(s) 17, 418, 989
BstNI CCWGG 2 cut(s) 806, 973
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 2 cut(s) 804, 971
BstUI CGCG 4 cut(s) 9, 37, 93, 445
BstV1I GCAGC 2 cut(s) 230, 624
BstV2I GAAGAC 1 cut(s) 345
BstXI CCANNNNNNTGG 2 cut(s) 388, 812
BsuRI GGCC 3 cut(s) 228, 318, 507
BtgI CCRYGG 1 cut(s) 205
BtgZI GCGATG 3 cut(s) 196, 327, 426
BtsCI GGATG 6 cut(s) 124, 429, 661, 702, 985, 1021
BtsIMutI CAGTG 3 cut(s) 109, 391, 858
BtuMI TCGCGA 1 cut(s) 445
Cac8I GCNNGC 2 cut(s) 9, 264
CfoI GCGC 6 cut(s) 9, 11, 37, 243, 787, 804
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 505
CseI GACGC 3 cut(s) 26, 26, 82
Csp6I GTAC 2 cut(s) 730, 753
CviAII CATG 3 cut(s) 263, 538, 693
CviQI GTAC 2 cut(s) 730, 753
DdeI CTNAG 4 cut(s) 509, 993, 1010, 1032
DpnI GATC 3 cut(s) 170, 634, 961
DpnII GATC 3 cut(s) 168, 632, 959
DraI TTTAAA 1 cut(s) 871
EaeI YGGCCR 1 cut(s) 316
Eam1104I CTCTTC 2 cut(s) 193, 872
EarI CTCTTC 2 cut(s) 193, 872
Eco31I GGTCTC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 1076
EcoRII CCWGG 2 cut(s) 804, 971
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 3 cut(s) 266, 541, 696
FatI CATG 3 cut(s) 262, 537, 692
FblI GTMKAC 1 cut(s) 83
Fnu4HI GCNGC 4 cut(s) 5, 33, 244, 638
FokI GGATG 6 cut(s) 111, 416, 668, 709, 992, 1028
Fsp4HI GCNGC 4 cut(s) 5, 33, 244, 638
FspBI CTAG 2 cut(s) 119, 770
GlaI GCGC 6 cut(s) 8, 10, 36, 242, 786, 803
GluI GCNGC 4 cut(s) 5, 33, 244, 638
HaeIII GGCC 3 cut(s) 228, 318, 507
HapII CCGG 1 cut(s) 290
HgaI GACGC 3 cut(s) 26, 26, 82
HhaI GCGC 6 cut(s) 9, 11, 37, 243, 787, 804
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 3 cut(s) 266, 541, 696
Hin6I GCGC 6 cut(s) 7, 9, 35, 241, 785, 802
HinP1I GCGC 6 cut(s) 7, 9, 35, 241, 785, 802
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HinfI GANTC 7 cut(s) 193, 341, 355, 488, 747, 934, 1030
HpaII CCGG 1 cut(s) 290
HphI GGTGA 3 cut(s) 69, 88, 679
Hpy166II GTNNAC 5 cut(s) 84, 334, 831, 1029, 1189
Hpy188I TCNGA 8 cut(s) 304, 365, 487, 947, 964, 996, 1035, 1120
Hpy188III TCNNGA 5 cut(s) 444, 630, 845, 1049, 1094
Hpy8I GTNNAC 5 cut(s) 84, 334, 831, 1029, 1189
Hpy99I CGWCG 1 cut(s) 256
HpyAV CCTTC 3 cut(s) 147, 695, 970
HpyCH4III ACNGT 4 cut(s) 81, 100, 530, 891
HpyCH4V TGCA 4 cut(s) 107, 688, 727, 1043
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 418, 989
HpyF3I CTNAG 4 cut(s) 509, 993, 1010, 1032
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 3 cut(s) 266, 541, 696
HspAI GCGC 6 cut(s) 7, 9, 35, 241, 785, 802
Kzo9I GATC 3 cut(s) 168, 632, 959
LguI GCTCTTC 1 cut(s) 193
LmnI GCTCC 4 cut(s) 139, 478, 563, 1072
Lsp1109I GCAGC 2 cut(s) 230, 624
LweI GCATC 3 cut(s) 219, 635, 970
MaeI CTAG 2 cut(s) 119, 770
MaeIII GTNAC 2 cut(s) 94, 524
MalI GATC 3 cut(s) 170, 634, 961
MboI GATC 3 cut(s) 168, 632, 959
MboII GAAGA 8 cut(s) 60, 180, 350, 367, 889, 895, 1082, 1157
MhlI GDGCHC 2 cut(s) 302, 1077
MlsI TGGCCA 1 cut(s) 318
MluCI AATT 9 cut(s) 174, 220, 424, 454, 609, 913, 1019, 1098, 1161
MluNI TGGCCA 1 cut(s) 318
MlyI GAGTC 2 cut(s) 335, 1024
MmeI TCCRAC 1 cut(s) 516
Mox20I TGGCCA 1 cut(s) 318
MroXI GAANNNNTTC 1 cut(s) 1164
MscI TGGCCA 1 cut(s) 318
MseI TTAA 8 cut(s) 395, 468, 515, 840, 870, 939, 1056, 1148
MslI CAYNNNNRTG 3 cut(s) 277, 386, 695
Msp20I TGGCCA 1 cut(s) 318
MspCI CTTAAG 1 cut(s) 938
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 2 cut(s) 806, 973
MvaI CCWGG 2 cut(s) 806, 973
MvnI CGCG 4 cut(s) 9, 37, 93, 445
MwoI GCNNNNNNNGC 3 cut(s) 17, 418, 989
NdeII GATC 3 cut(s) 168, 632, 959
NlaIII CATG 3 cut(s) 266, 541, 696
NlaIV GGNNCC 1 cut(s) 559
NmuCI GTSAC 2 cut(s) 94, 524
NruI TCGCGA 1 cut(s) 445
NspI RCATGY 1 cut(s) 266
OliI CACNNNNGTG 1 cut(s) 386
PaeI GCATGC 1 cut(s) 266
PalAI GGCGCGCC 1 cut(s) 7
PauI GCGCGC 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 1164
PfeI GAWTC 5 cut(s) 193, 355, 488, 747, 934
PflFI GACNNNGTC 1 cut(s) 1184
PkrI GCNGC 4 cut(s) 6, 34, 245, 639
PleI GAGTC 2 cut(s) 335, 1024
PpsI GAGTC 2 cut(s) 335, 1024
PshBI ATTAAT 1 cut(s) 840
Psp6I CCWGG 2 cut(s) 804, 971
PspGI CCWGG 2 cut(s) 804, 971
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 1184
PteI GCGCGC 1 cut(s) 7
RruI TCGCGA 1 cut(s) 445
RsaI GTAC 2 cut(s) 731, 754
RsaNI GTAC 2 cut(s) 730, 753
RseI CAYNNNNRTG 3 cut(s) 277, 386, 695
SapI GCTCTTC 1 cut(s) 193
SaqAI TTAA 8 cut(s) 395, 468, 515, 840, 870, 939, 1056, 1148
SatI GCNGC 4 cut(s) 5, 33, 244, 638
Sau3AI GATC 3 cut(s) 168, 632, 959
Sau96I GGNCC 1 cut(s) 505
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 2 cut(s) 335, 1024
ScrFI CCNGG 2 cut(s) 806, 973
SduI GDGCHC 2 cut(s) 302, 1077
SfaNI GCATC 3 cut(s) 219, 635, 970
SgsI GGCGCGCC 1 cut(s) 7
SmiMI CAYNNNNRTG 3 cut(s) 277, 386, 695
SmlI CTYRAG 2 cut(s) 26, 938
SmoI CTYRAG 2 cut(s) 26, 938
SphI GCATGC 1 cut(s) 266
Sse9I AATT 9 cut(s) 174, 220, 424, 454, 609, 913, 1019, 1098, 1161
SsiI CCGC 3 cut(s) 4, 32, 824
SspI AATATT 1 cut(s) 1081
SspMI CTAG 2 cut(s) 119, 770
StyD4I CCNGG 2 cut(s) 804, 971
TaaI ACNGT 4 cut(s) 81, 100, 530, 891
TaqI TCGA 3 cut(s) 167, 191, 353
TaqII GACCGA 1 cut(s) 244
TasI AATT 9 cut(s) 174, 220, 424, 454, 609, 913, 1019, 1098, 1161
TatI WGTACW 1 cut(s) 729
TauI GCSGC 2 cut(s) 7, 35
TfiI GAWTC 5 cut(s) 193, 355, 488, 747, 934
Tru1I TTAA 8 cut(s) 395, 468, 515, 840, 870, 939, 1056, 1148
Tru9I TTAA 8 cut(s) 395, 468, 515, 840, 870, 939, 1056, 1148
TscAI CASTG 3 cut(s) 109, 391, 865
TseFI GTSAC 2 cut(s) 94, 524
TseI GCWGC 2 cut(s) 243, 637
Tsp45I GTSAC 2 cut(s) 94, 524
TspDTI ATGAA 3 cut(s) 418, 890, 1032
TspGWI ACGGA 2 cut(s) 569, 740
TspRI CASTG 3 cut(s) 109, 391, 865
Tth111I GACNNNGTC 1 cut(s) 1184
Vha464I CTTAAG 1 cut(s) 938
VspI ATTAAT 1 cut(s) 840
XapI RAATTY 5 cut(s) 424, 913, 1019, 1098, 1161
XceI RCATGY 1 cut(s) 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 1164
XspI CTAG 2 cut(s) 119, 770
ZrmI AGTACT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.