Rw1G028630

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014410
Physical Location & Seq
Reverse (-)
56307098 .. 56310518
3421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G028630.1

Sequence Viewer

Length: 1269 bp
ATGCGGCGCGCCATAGACGCTCACCGCAAGACCATGCGTCTCTTTCCTCCCCTCTCTTCCTTCTCCTCTTCTTCTTCTCCTCCTTCTTCTTCTTCCACGCCTCTCTTCTCCGTCCGTACATTTCTGACCCTAGCCCAGCCGCGCTCCGTTTCCAATTCCGTCTCCAAAACCCTAATTTTCCACTCAATCGCTACTCCTTCCTACACTTCTTCTTCTTCTTCTTCTTCTTCCTCCTCATCGATTCCCAGCCTCAGAAGCCACAGACTTGGGCCGGCTATTGGGCTCTTTGCCGTGCGGTGCGTCTCGACGGCCTCGCCGGAGAGCAGTTTGGACTGGAACGAGCCGGTGTCGTGCTCCGAGGTCGGCGACGGCAGCAAAGGAAGTGTTGATAAGGATGATAAGCCCAATATTCCGGTCAGAGCTTACTTCTTTTCCACTAGTGTGGATTTGAGAAGCTTGGTGGAGCAGAACAGGCTGAATTTCATCCCTCCTACGTCGCGAATGACCAATTATGTTGTGTTTAAATTCGGGGAGCTTACCAGGTCTGACAATGCTTCGGGTCCTAGTTTAAGTGGGAGCGACTGTTGCTACATGGTAGTTTTTCAATATGGGTCCATTGTCTTGTTTAATGTTGGTGAACATGAGATTGATGGGTACTTGAAAATTGTGGAAAAACATGCATCTGGATTGCTACCTGAAATGAGAAAGGATGAGTATGAGGTGAGAGAGAAACCAGATTTAGACACATGGATGGAAGGCGGATTGGATTACATAATGTTGCAGCACTTGAATACTGATGGTATTCGTACCATTGGTAGTGTTCTTGGTCAAAGTATTGCTCTGGATTACTACGTCCGTCAGGTTGATGGAATGGTTGCAGAGTTTACTGACATTAATCGTGAAATGGAAACTACTGGAAAATTTAAGATGAAGAGGAAGAAACTATTTCAACTGGTGGGGAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTTGGACTTTTTGAGAGATCAGACATTGCCTGGAAGGATGCCAAATATGCTCAGATTTGGGAATACCTCAGGGATGAATTTGAATTAACTCAGAGATTTGCTAGTCTTGACTTTAAGTTGAAGTTTGTGGAGCACAATATTCGCTTTCTTCAGGAAATTCTTCAAAACCGGAAATCAGATTTTTTGGAGTGGCTCATCATTGCACTAATAGGTGCTGAGATTATGCTCTCTCTTTATGACATTCTCCACAGATCAGCACTCGCTTCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

422

Amino Acids

47.4

Weight (kDa)

6.73

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 198 - 368 1.4e-34 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13610
fragaria_vesca FvH4_7g20360
malus_domestica MD01G1113300.v1.1 MD07G1180700.v1.1
prunus_persica Prupe.2G220000_v2.0.a1
pyrus_communis pycom01g14160 pycom07g17400
rosa_chinensis RchiOBHm_Chr1g0364321
rosa_laevigata RLG00000027495
rosa_multiflora Rmu_sc0007470.1_g000002
rosa_roxburghii Rroxscaffold_4G00292430
rosa_rugosa Rorug01G0315400
rosa_samantha Rh1AG323100 Rh1BG285700 Rh1CG302400 Rh1DG317200
rosa_wichuraiana Rw1G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 9, 142, 499
AciI CCGC 5 cut(s) 4, 25, 140, 295, 759
AcsI RAATTY 6 cut(s) 478, 524, 920, 967, 1073, 1152
AcuI CTGAAG 1 cut(s) 1130
AfaI GTAC 3 cut(s) 118, 656, 808
AfiI CCNNNNNNNGG 1 cut(s) 278
AflII CTTAAG 1 cut(s) 992
AgsI TTSAA 7 cut(s) 605, 661, 790, 950, 1079, 1117, 1160
AhdI GACNNNNNGTC 1 cut(s) 36
AhlI ACTAGT 1 cut(s) 437
AjnI CCWGG 2 cut(s) 539, 1025
AleI CACNNNNGTG 1 cut(s) 440
AluBI AGCT 4 cut(s) 422, 456, 535, 997
AluI AGCT 4 cut(s) 422, 456, 535, 997
Alw21I GWGCWC 2 cut(s) 356, 1131
Alw26I GTCTC 3 cut(s) 44, 166, 307
AoxI GGCC 2 cut(s) 269, 309
ApeKI GCWGC 2 cut(s) 372, 781
ApoI RAATTY 6 cut(s) 478, 524, 920, 967, 1073, 1152
AscI GGCGCGCC 1 cut(s) 7
AseI ATTAAT 1 cut(s) 894
Asp700I GAANNNNTTC 1 cut(s) 1155
AspLEI GCGC 3 cut(s) 9, 11, 144
AspS9I GGNCC 3 cut(s) 269, 560, 612
AsuHPI GGTGA 3 cut(s) 14, 647, 733
AvaII GGWCC 2 cut(s) 560, 612
AxyI CCTNAGG 1 cut(s) 1064
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 2 cut(s) 356, 1131
BbvI GCAGC 2 cut(s) 384, 793
BccI CCATC 4 cut(s) 644, 745, 791, 860
BceAI ACGGC 3 cut(s) 275, 324, 385
BcgI CGANNNNNNTGC 2 cut(s) 1118, 1152
BciT130I CCWGG 2 cut(s) 541, 1027
BcoDI GTCTC 3 cut(s) 44, 166, 307
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 4 cut(s) 131, 438, 564, 1098
BfrI CTTAAG 1 cut(s) 992
BisI GCNGC 4 cut(s) 5, 140, 373, 782
BlsI GCNGC 4 cut(s) 6, 141, 374, 783
Bme1390I CCNGG 2 cut(s) 541, 1027
Bme18I GGWCC 2 cut(s) 560, 612
BmeRI GACNNNNNGTC 1 cut(s) 36
BmgT120I GGNCC 3 cut(s) 269, 560, 612
BmiI GGNNCC 2 cut(s) 561, 613
BmrFI CCNGG 2 cut(s) 541, 1027
BmsI GCATC 2 cut(s) 689, 1024
Bsa29I ATCGAT 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 357
BsaWI WCCGGW 2 cut(s) 412, 1164
BsaXI ACNNNNNCTCC 2 cut(s) 568, 598
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse118I RCCGGY 2 cut(s) 271, 343
Bse1I ACTGG 3 cut(s) 338, 919, 957
Bse21I CCTNAGG 1 cut(s) 1064
Bse3DI GCAATG 2 cut(s) 1020, 1194
BseBI CCWGG 2 cut(s) 541, 1027
BseCI ATCGAT 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 6 cut(s) 400, 483, 715, 756, 1039, 1075
BseLI CCNNNNNNNGG 1 cut(s) 278
BseMI GCAATG 2 cut(s) 1020, 1194
BseMII CTCAG 5 cut(s) 265, 1061, 1078, 1100, 1203
BseNI ACTGG 3 cut(s) 338, 919, 957
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 3 cut(s) 55, 69, 223
BseXI GCAGC 2 cut(s) 384, 793
BseYI CCCAGC 2 cut(s) 135, 245
Bsh1236I CGCG 3 cut(s) 9, 142, 499
BshFI GGCC 2 cut(s) 271, 311
BshVI ATCGAT 1 cut(s) 239
BsiHKAI GWGCWC 2 cut(s) 356, 1131
BsiSI CCGG 5 cut(s) 272, 317, 344, 413, 1165
BslI CCNNNNNNNGG 1 cut(s) 278
BsmAI GTCTC 3 cut(s) 44, 166, 307
BsmBI CGTCTC 3 cut(s) 44, 166, 307
BsnI GGCC 2 cut(s) 271, 311
Bsp1286I GDGCHC 3 cut(s) 285, 356, 1131
Bsp143I GATC 2 cut(s) 1013, 1247
Bsp68I TCGCGA 1 cut(s) 499
BspACI CCGC 5 cut(s) 4, 25, 140, 295, 759
BspANI GGCC 2 cut(s) 271, 311
BspCNI CTCAG 5 cut(s) 264, 1060, 1077, 1099, 1204
BspDI ATCGAT 1 cut(s) 239
BspFNI CGCG 3 cut(s) 9, 142, 499
BspLI GGNNCC 2 cut(s) 561, 613
BspTI CTTAAG 1 cut(s) 992
BsrDI GCAATG 2 cut(s) 1020, 1194
BsrFI RCCGGY 2 cut(s) 271, 343
BsrI ACTGG 3 cut(s) 338, 919, 957
BssAI RCCGGY 2 cut(s) 271, 343
BssECI CCNNGG 1 cut(s) 357
BssHII GCGCGC 1 cut(s) 7
BssMI GATC 2 cut(s) 1013, 1247
Bst2UI CCWGG 2 cut(s) 541, 1027
Bst4CI ACNGT 1 cut(s) 584
Bst6I CTCTTC 4 cut(s) 61, 73, 110, 926
BstAFI CTTAAG 1 cut(s) 992
BstC8I GCNNGC 2 cut(s) 9, 273
BstDEI CTNAG 5 cut(s) 251, 1047, 1064, 1086, 1212
BstF5I GGATG 6 cut(s) 400, 483, 715, 756, 1039, 1075
BstFNI CGCG 3 cut(s) 9, 142, 499
BstHHI GCGC 3 cut(s) 9, 11, 144
BstKTI GATC 2 cut(s) 1016, 1250
BstMAI GTCTC 3 cut(s) 44, 166, 307
BstMBI GATC 2 cut(s) 1013, 1247
BstMWI GCNNNNNNNGC 6 cut(s) 17, 255, 372, 472, 585, 1043
BstNI CCWGG 2 cut(s) 541, 1027
BstNSI RCATGY 1 cut(s) 680
BstSCI CCNGG 2 cut(s) 539, 1025
BstUI CGCG 3 cut(s) 9, 142, 499
BstV1I GCAGC 2 cut(s) 384, 793
BstXI CCANNNNNNTGG 2 cut(s) 266, 442
Bsu15I ATCGAT 1 cut(s) 239
Bsu36I CCTNAGG 1 cut(s) 1064
BsuRI GGCC 2 cut(s) 271, 311
BsuTUI ATCGAT 1 cut(s) 239
BtsCI GGATG 6 cut(s) 400, 483, 715, 756, 1039, 1075
BtuMI TCGCGA 1 cut(s) 499
Cac8I GCNNGC 2 cut(s) 9, 273
CfoI GCGC 3 cut(s) 9, 11, 144
Cfr10I RCCGGY 2 cut(s) 271, 343
Cfr13I GGNCC 3 cut(s) 269, 560, 612
ClaI ATCGAT 1 cut(s) 239
CseI GACGC 3 cut(s) 26, 26, 289
CsiI ACCWGGT 1 cut(s) 539
Csp6I GTAC 3 cut(s) 117, 655, 807
CviAII CATG 5 cut(s) 34, 592, 641, 677, 747
CviQI GTAC 3 cut(s) 117, 655, 807
DdeI CTNAG 5 cut(s) 251, 1047, 1064, 1086, 1212
DpnI GATC 2 cut(s) 1015, 1249
DpnII GATC 2 cut(s) 1013, 1247
DraI TTTAAA 1 cut(s) 523
DriI GACNNNNNGTC 1 cut(s) 36
Eam1104I CTCTTC 4 cut(s) 61, 73, 110, 926
Eam1105I GACNNNNNGTC 1 cut(s) 36
EarI CTCTTC 4 cut(s) 61, 73, 110, 926
EciI GGCGGA 1 cut(s) 774
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 2 cut(s) 560, 612
Eco57I CTGAAG 1 cut(s) 1130
Eco81I CCTNAGG 1 cut(s) 1064
EcoO109I RGGNCCY 1 cut(s) 560
EcoRII CCWGG 2 cut(s) 539, 1025
EcoT22I ATGCAT 1 cut(s) 682
EcoT38I GRGCYC 1 cut(s) 285
Esp3I CGTCTC 3 cut(s) 44, 166, 307
FaeI CATG 5 cut(s) 37, 595, 644, 680, 750
FatI CATG 5 cut(s) 33, 591, 640, 676, 746
Fnu4HI GCNGC 4 cut(s) 5, 140, 373, 782
FokI GGATG 6 cut(s) 407, 470, 722, 763, 1046, 1082
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 4 cut(s) 5, 140, 373, 782
FspBI CTAG 4 cut(s) 131, 438, 564, 1098
GlaI GCGC 3 cut(s) 8, 10, 143
GluI GCNGC 4 cut(s) 5, 140, 373, 782
GsaI CCCAGC 2 cut(s) 139, 249
HaeIII GGCC 2 cut(s) 271, 311
HapII CCGG 5 cut(s) 272, 317, 344, 413, 1165
HgaI GACGC 3 cut(s) 26, 26, 289
HhaI GCGC 3 cut(s) 9, 11, 144
Hin1II CATG 5 cut(s) 37, 595, 644, 680, 750
Hin6I GCGC 3 cut(s) 7, 9, 142
HinP1I GCGC 3 cut(s) 7, 9, 142
HindIII AAGCTT 2 cut(s) 454, 995
HinfI GANTC 2 cut(s) 241, 988
HpaII CCGG 5 cut(s) 272, 317, 344, 413, 1165
HphI GGTGA 3 cut(s) 14, 647, 733
Hpy166II GTNNAC 2 cut(s) 638, 885
Hpy188I TCNGA 9 cut(s) 126, 254, 358, 419, 547, 1018, 1050, 1089, 1174
Hpy188III TCNNGA 7 cut(s) 304, 498, 684, 842, 899, 1103, 1148
Hpy8I GTNNAC 2 cut(s) 638, 885
Hpy99I CGWCG 3 cut(s) 310, 371, 499
HpyAV CCTTC 6 cut(s) 70, 93, 207, 749, 955, 1024
HpyCH4III ACNGT 1 cut(s) 584
HpyCH4IV ACGT 2 cut(s) 494, 852
HpyCH4V TGCA 4 cut(s) 680, 781, 878, 1199
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 255, 372, 472, 585, 1043
HpyF3I CTNAG 5 cut(s) 251, 1047, 1064, 1086, 1212
HpySE526I ACGT 2 cut(s) 494, 852
Hsp92II CATG 5 cut(s) 37, 595, 644, 680, 750
HspAI GCGC 3 cut(s) 7, 9, 142
KroI GCCGGC 1 cut(s) 271
KroNI GCCGGC 1 cut(s) 273
Kzo9I GATC 2 cut(s) 1013, 1247
LmnI GCTCC 6 cut(s) 149, 359, 463, 532, 576, 1126
Lsp1109I GCAGC 2 cut(s) 384, 793
LweI GCATC 2 cut(s) 689, 1024
MabI ACCWGGT 1 cut(s) 539
MaeI CTAG 4 cut(s) 131, 438, 564, 1098
MaeII ACGT 2 cut(s) 494, 852
MalI GATC 2 cut(s) 1015, 1249
MboI GATC 2 cut(s) 1013, 1247
MhlI GDGCHC 3 cut(s) 285, 356, 1131
Mph1103I ATGCAT 1 cut(s) 682
MroNI GCCGGC 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 1155
MseI TTAA 8 cut(s) 522, 569, 627, 894, 924, 993, 1082, 1110
MslI CAYNNNNRTG 2 cut(s) 440, 749
MspCI CTTAAG 1 cut(s) 992
MspI CCGG 5 cut(s) 272, 317, 344, 413, 1165
MspR9I CCNGG 2 cut(s) 541, 1027
MvaI CCWGG 2 cut(s) 541, 1027
MvnI CGCG 3 cut(s) 9, 142, 499
MwoI GCNNNNNNNGC 6 cut(s) 17, 255, 372, 472, 585, 1043
NaeI GCCGGC 1 cut(s) 273
NdeII GATC 2 cut(s) 1013, 1247
NgoMIV GCCGGC 1 cut(s) 271
NlaIII CATG 5 cut(s) 37, 595, 644, 680, 750
NlaIV GGNNCC 2 cut(s) 561, 613
NruI TCGCGA 1 cut(s) 499
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 1 cut(s) 680
OliI CACNNNNGTG 1 cut(s) 440
PalAI GGCGCGCC 1 cut(s) 7
PauI GCGCGC 1 cut(s) 7
PdiI GCCGGC 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 1155
PfeI GAWTC 2 cut(s) 241, 988
PkrI GCNGC 4 cut(s) 6, 141, 374, 783
PpuMI RGGWCCY 1 cut(s) 560
PshBI ATTAAT 1 cut(s) 894
Psp5II RGGWCCY 1 cut(s) 560
Psp6I CCWGG 2 cut(s) 539, 1025
PspFI CCCAGC 2 cut(s) 135, 245
PspGI CCWGG 2 cut(s) 539, 1025
PspN4I GGNNCC 2 cut(s) 561, 613
PspPI GGNCC 3 cut(s) 269, 560, 612
PspPPI RGGWCCY 1 cut(s) 560
PteI GCGCGC 1 cut(s) 7
RruI TCGCGA 1 cut(s) 499
RsaI GTAC 3 cut(s) 118, 656, 808
RsaNI GTAC 3 cut(s) 117, 655, 807
RseI CAYNNNNRTG 2 cut(s) 440, 749
SaqAI TTAA 8 cut(s) 522, 569, 627, 894, 924, 993, 1082, 1110
SatI GCNGC 4 cut(s) 5, 140, 373, 782
Sau3AI GATC 2 cut(s) 1013, 1247
Sau96I GGNCC 3 cut(s) 269, 560, 612
ScrFI CCNGG 2 cut(s) 541, 1027
SduI GDGCHC 3 cut(s) 285, 356, 1131
SexAI ACCWGGT 1 cut(s) 539
SfaNI GCATC 2 cut(s) 689, 1024
SgsI GGCGCGCC 1 cut(s) 7
SinI GGWCC 2 cut(s) 560, 612
SmiMI CAYNNNNRTG 2 cut(s) 440, 749
SmlI CTYRAG 1 cut(s) 992
SmoI CTYRAG 1 cut(s) 992
SpeI ACTAGT 1 cut(s) 437
SsiI CCGC 5 cut(s) 4, 25, 140, 295, 759
SspI AATATT 2 cut(s) 409, 1135
SspMI CTAG 4 cut(s) 131, 438, 564, 1098
StyD4I CCNGG 2 cut(s) 539, 1025
TaaI ACNGT 1 cut(s) 584
TaiI ACGT 2 cut(s) 497, 855
TaqI TCGA 2 cut(s) 239, 305
TauI GCSGC 2 cut(s) 7, 142
TfiI GAWTC 2 cut(s) 241, 988
Tru1I TTAA 8 cut(s) 522, 569, 627, 894, 924, 993, 1082, 1110
Tru9I TTAA 8 cut(s) 522, 569, 627, 894, 924, 993, 1082, 1110
TseI GCWGC 2 cut(s) 372, 781
TspDTI ATGAA 3 cut(s) 472, 944, 1086
TspGWI ACGGA 5 cut(s) 100, 104, 136, 148, 845
Vha464I CTTAAG 1 cut(s) 992
VpaK11BI GGWCC 2 cut(s) 560, 612
VspI ATTAAT 1 cut(s) 894
XapI RAATTY 6 cut(s) 478, 524, 920, 967, 1073, 1152
XceI RCATGY 1 cut(s) 680
XmnI GAANNNNTTC 1 cut(s) 1155
XspI CTAG 4 cut(s) 131, 438, 564, 1098
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.