Rroxscaffold_4G00292430

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014410
Physical Location & Seq
Forward (+)
12747402 .. 12750837
3436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00292430.1

Sequence Viewer

Length: 1185 bp
ATGCGGCGCGCCATAGACGCCCACCTCAAGACCATGCGTCTCTTTCCTCCCCTCTCTTCCTTCTCCTCTTCTTCTCCTCCTCCTTCTTCTTCCACGCCTCTCTTCTCCGTCCGTACATTTCTGACCCTAGCCCAGCCGCGCTCCGTTTCCAATTCCGTCTCCAAAACCCTAATTTTCCACTCAATCGCCACTCCTTCCTACACTTCTTCTTCTTCCTCATCGATTCCCAGCCTCAGAAGCCACAGAATTGGGCCGGGTATTGGGCTGTTTTCCGTGCGGTGCGTTTCAACGGCCTCGCCGGAGAGCAGTTTGGACTGGAACGAGCCGGTGTCGTGCTCCGAGGTCGGCGACGGCAGCAAAGGAAGTGTTGATAAGGATGATAAGCCCAATATTCCGGTCAGAGCTTACTTCTTTTCCACTAGGCTTCTTATAGGCGCTGTTGGTTTATGTGTCCTTTTGCAGCCTGTTGCTTCGGGTCCTAGTTTAAGTGGGAGCGACTGTTGCTACATGGTAGTTTTTCAATATGGGTCCATTGTCTTGTTTAATGTTCGTGAACATGAGATTGATGGGTACTTGAAAATTGTGGAAAAACATGCATCTGGATTGCTACCTGAAATGAGAAAGGATGAGTATGAGGTGAGAGAGAAACCAGATTTAGACACATGGATGGAAGGCGGATTGGATTACATAATGTTGCAGCACTTGAATACTGATGGTATTCGTACCATTGGTAGTGTTCTTGGTCAGAGTATTGCTCTGGATTACTACGTCCGCCAGGTTGATGGAATGGTTGCAGAGTTTACTGACATTAATCGTGAAATGGAAACTACTGGAAAATTTAAGATGAAGAGGAAGAAACTATTTCAGCTGGTGGGGAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTTGGACTTTTTGAGAGATCAGACATTGCCTGGAAGGATGCCAAATATGCACAGATATGGGAATACCTCAGGGATGAATTTGAATTAACTCAGAGATTTGCTAGTCTTGACTTTAAGTTGAAGTTTGTGGAGCACAATATTCGCTTTCTTCAGGAAATTCTTCAAAACCGGAAATCAGATTTTTTGGAGTGGCTCATCATTGCACTAATAGGTGCTGAGATTATGCTCTCTCTTTATGACATTCTCCACAGATCAGCACTTGCTTCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

394

Amino Acids

44.16

Weight (kDa)

6.72

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 170 - 340 8.5e-35 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13610
fragaria_vesca FvH4_7g20360
malus_domestica MD01G1113300.v1.1 MD07G1180700.v1.1
prunus_persica Prupe.2G220000_v2.0.a1
pyrus_communis pycom01g14160 pycom07g17400
rosa_chinensis RchiOBHm_Chr1g0364321
rosa_laevigata RLG00000027495
rosa_multiflora Rmu_sc0007470.1_g000002
rosa_roxburghii Rroxscaffold_4G00292430
rosa_rugosa Rorug01G0315400
rosa_samantha Rh1AG323100 Rh1BG285700 Rh1CG302400 Rh1DG317200
rosa_wichuraiana Rw1G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 9, 139
AciI CCGC 5 cut(s) 4, 137, 277, 675, 772
AcsI RAATTY 4 cut(s) 836, 883, 989, 1068
AcuI CTGAAG 1 cut(s) 1046
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 3 cut(s) 115, 572, 724
AfiI CCNNNNNNNGG 1 cut(s) 260
AflII CTTAAG 1 cut(s) 908
AgsI TTSAA 7 cut(s) 288, 521, 577, 706, 995, 1033, 1076
AhdI GACNNNNNGTC 1 cut(s) 36
AjnI CCWGG 2 cut(s) 774, 941
AluBI AGCT 3 cut(s) 404, 868, 913
AluI AGCT 3 cut(s) 404, 868, 913
Alw21I GWGCWC 2 cut(s) 338, 1047
Alw26I GTCTC 2 cut(s) 44, 163
AoxI GGCC 2 cut(s) 251, 291
ApeKI GCWGC 3 cut(s) 354, 460, 697
ApoI RAATTY 4 cut(s) 836, 883, 989, 1068
AscI GGCGCGCC 1 cut(s) 7
AseI ATTAAT 1 cut(s) 810
Asp700I GAANNNNTTC 1 cut(s) 1071
AspLEI GCGC 4 cut(s) 9, 11, 141, 437
AspS9I GGNCC 3 cut(s) 251, 476, 528
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 649
AvaII GGWCC 2 cut(s) 476, 528
AxyI CCTNAGG 1 cut(s) 980
Bbv12I GWGCWC 2 cut(s) 338, 1047
BbvI GCAGC 3 cut(s) 366, 472, 709
BccI CCATC 4 cut(s) 560, 661, 707, 776
BceAI ACGGC 2 cut(s) 306, 367
BcgI CGANNNNNNTGC 2 cut(s) 1034, 1068
BciT130I CCWGG 2 cut(s) 776, 943
BcnI CCSGG 1 cut(s) 255
BcoDI GTCTC 2 cut(s) 44, 163
BfaI CTAG 4 cut(s) 128, 420, 480, 1014
BfoI RGCGCY 1 cut(s) 438
BfrI CTTAAG 1 cut(s) 908
BisI GCNGC 5 cut(s) 5, 137, 355, 461, 698
BlsI GCNGC 5 cut(s) 6, 138, 356, 462, 699
Bme1390I CCNGG 3 cut(s) 255, 776, 943
Bme18I GGWCC 2 cut(s) 476, 528
BmeRI GACNNNNNGTC 1 cut(s) 36
BmgT120I GGNCC 3 cut(s) 251, 476, 528
BmiI GGNNCC 2 cut(s) 477, 529
BmrFI CCNGG 3 cut(s) 255, 776, 943
BmsI GCATC 2 cut(s) 605, 940
BplI GAGNNNNNCTC 2 cut(s) 739, 771
BpuEI CTTGAG 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 221
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 339
BsaWI WCCGGW 2 cut(s) 394, 1080
BsaXI ACNNNNNCTCC 2 cut(s) 484, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 320, 835
Bse21I CCTNAGG 1 cut(s) 980
Bse3DI GCAATG 2 cut(s) 936, 1110
BseBI CCWGG 2 cut(s) 776, 943
BseCI ATCGAT 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 339
BseGI GGATG 5 cut(s) 382, 631, 672, 955, 991
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 2 cut(s) 936, 1110
BseMII CTCAG 4 cut(s) 247, 994, 1016, 1119
BseNI ACTGG 2 cut(s) 320, 835
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 3 cut(s) 55, 66, 69
BseXI GCAGC 3 cut(s) 366, 472, 709
BseYI CCCAGC 2 cut(s) 132, 227
Bsh1236I CGCG 2 cut(s) 9, 139
BshFI GGCC 2 cut(s) 253, 293
BshVI ATCGAT 1 cut(s) 221
BsiHKAI GWGCWC 2 cut(s) 338, 1047
BsiSI CCGG 5 cut(s) 254, 299, 326, 395, 1081
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 2 cut(s) 44, 163
BsmBI CGTCTC 2 cut(s) 44, 163
BsnI GGCC 2 cut(s) 253, 293
Bsp1286I GDGCHC 2 cut(s) 338, 1047
Bsp143I GATC 2 cut(s) 929, 1163
BspACI CCGC 5 cut(s) 4, 137, 277, 675, 772
BspANI GGCC 2 cut(s) 253, 293
BspCNI CTCAG 4 cut(s) 246, 993, 1015, 1120
BspDI ATCGAT 1 cut(s) 221
BspFNI CGCG 2 cut(s) 9, 139
BspLI GGNNCC 2 cut(s) 477, 529
BspTI CTTAAG 1 cut(s) 908
BsrDI GCAATG 2 cut(s) 936, 1110
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 2 cut(s) 320, 835
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 339
BssHII GCGCGC 1 cut(s) 7
BssMI GATC 2 cut(s) 929, 1163
BssNI GRCGYC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 776, 943
Bst4CI ACNGT 1 cut(s) 500
Bst6I CTCTTC 4 cut(s) 61, 73, 107, 842
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 908
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 4 cut(s) 233, 980, 1002, 1128
BstF5I GGATG 5 cut(s) 382, 631, 672, 955, 991
BstFNI CGCG 2 cut(s) 9, 139
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 4 cut(s) 9, 11, 141, 437
BstKTI GATC 2 cut(s) 932, 1166
BstMAI GTCTC 2 cut(s) 44, 163
BstMBI GATC 2 cut(s) 929, 1163
BstMWI GCNNNNNNNGC 5 cut(s) 17, 237, 354, 501, 959
BstNI CCWGG 2 cut(s) 776, 943
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 3 cut(s) 253, 774, 941
BstUI CGCG 2 cut(s) 9, 139
BstV1I GCAGC 3 cut(s) 366, 472, 709
BstXI CCANNNNNNTGG 2 cut(s) 248, 782
Bsu15I ATCGAT 1 cut(s) 221
Bsu36I CCTNAGG 1 cut(s) 980
BsuRI GGCC 2 cut(s) 253, 293
BsuTUI ATCGAT 1 cut(s) 221
BtsCI GGATG 5 cut(s) 382, 631, 672, 955, 991
Cac8I GCNNGC 1 cut(s) 9
CfoI GCGC 4 cut(s) 9, 11, 141, 437
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 3 cut(s) 251, 476, 528
ClaI ATCGAT 1 cut(s) 221
CseI GACGC 2 cut(s) 26, 26
Csp6I GTAC 3 cut(s) 114, 571, 723
CviAII CATG 5 cut(s) 34, 508, 557, 593, 663
CviQI GTAC 3 cut(s) 114, 571, 723
DdeI CTNAG 4 cut(s) 233, 980, 1002, 1128
DpnI GATC 2 cut(s) 931, 1165
DpnII GATC 2 cut(s) 929, 1163
DriI GACNNNNNGTC 1 cut(s) 36
Eam1104I CTCTTC 4 cut(s) 61, 73, 107, 842
Eam1105I GACNNNNNGTC 1 cut(s) 36
EarI CTCTTC 4 cut(s) 61, 73, 107, 842
EciI GGCGGA 2 cut(s) 690, 761
Eco47I GGWCC 2 cut(s) 476, 528
Eco57I CTGAAG 1 cut(s) 1046
Eco81I CCTNAGG 1 cut(s) 980
EcoO109I RGGNCCY 1 cut(s) 476
EcoRII CCWGG 2 cut(s) 774, 941
EcoT22I ATGCAT 1 cut(s) 598
Esp3I CGTCTC 2 cut(s) 44, 163
FaeI CATG 5 cut(s) 37, 511, 560, 596, 666
FatI CATG 5 cut(s) 33, 507, 556, 592, 662
Fnu4HI GCNGC 5 cut(s) 5, 137, 355, 461, 698
FokI GGATG 5 cut(s) 389, 638, 679, 962, 998
Fsp4HI GCNGC 5 cut(s) 5, 137, 355, 461, 698
FspBI CTAG 4 cut(s) 128, 420, 480, 1014
GlaI GCGC 4 cut(s) 8, 10, 140, 436
GluI GCNGC 5 cut(s) 5, 137, 355, 461, 698
GsaI CCCAGC 2 cut(s) 136, 231
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 2 cut(s) 253, 293
HapII CCGG 5 cut(s) 254, 299, 326, 395, 1081
HgaI GACGC 2 cut(s) 26, 26
HhaI GCGC 4 cut(s) 9, 11, 141, 437
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 5 cut(s) 37, 511, 560, 596, 666
Hin6I GCGC 4 cut(s) 7, 9, 139, 435
HinP1I GCGC 4 cut(s) 7, 9, 139, 435
HindIII AAGCTT 1 cut(s) 911
HinfI GANTC 2 cut(s) 223, 904
HpaII CCGG 5 cut(s) 254, 299, 326, 395, 1081
HphI GGTGA 1 cut(s) 649
Hpy166II GTNNAC 2 cut(s) 554, 801
Hpy188I TCNGA 8 cut(s) 123, 236, 340, 401, 747, 934, 1005, 1090
Hpy188III TCNNGA 7 cut(s) 28, 551, 600, 758, 815, 1019, 1064
Hpy8I GTNNAC 2 cut(s) 554, 801
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 6 cut(s) 70, 93, 204, 665, 871, 940
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4IV ACGT 1 cut(s) 768
HpyCH4V TGCA 6 cut(s) 460, 596, 697, 794, 962, 1115
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 237, 354, 501, 959
HpyF3I CTNAG 4 cut(s) 233, 980, 1002, 1128
HpySE526I ACGT 1 cut(s) 768
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 5 cut(s) 37, 511, 560, 596, 666
HspAI GCGC 4 cut(s) 7, 9, 139, 435
Kzo9I GATC 2 cut(s) 929, 1163
LmnI GCTCC 4 cut(s) 146, 341, 492, 1042
Lsp1109I GCAGC 3 cut(s) 366, 472, 709
LweI GCATC 2 cut(s) 605, 940
MaeI CTAG 4 cut(s) 128, 420, 480, 1014
MaeII ACGT 1 cut(s) 768
MalI GATC 2 cut(s) 931, 1165
MboI GATC 2 cut(s) 929, 1163
MhlI GDGCHC 2 cut(s) 338, 1047
MluCI AATT 9 cut(s) 151, 171, 246, 579, 836, 883, 989, 995, 1068
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 1071
MseI TTAA 7 cut(s) 485, 543, 810, 840, 909, 998, 1026
MslI CAYNNNNRTG 2 cut(s) 665, 967
MspA1I CMGCKG 1 cut(s) 868
MspCI CTTAAG 1 cut(s) 908
MspI CCGG 5 cut(s) 254, 299, 326, 395, 1081
MspR9I CCNGG 3 cut(s) 255, 776, 943
MvaI CCWGG 2 cut(s) 776, 943
MvnI CGCG 2 cut(s) 9, 139
MwoI GCNNNNNNNGC 5 cut(s) 17, 237, 354, 501, 959
NciI CCSGG 1 cut(s) 255
NdeII GATC 2 cut(s) 929, 1163
NlaIII CATG 5 cut(s) 37, 511, 560, 596, 666
NlaIV GGNNCC 2 cut(s) 477, 529
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 1 cut(s) 596
PalAI GGCGCGCC 1 cut(s) 7
PauI GCGCGC 1 cut(s) 7
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 2 cut(s) 223, 904
PkrI GCNGC 5 cut(s) 6, 138, 356, 462, 699
PpuMI RGGWCCY 1 cut(s) 476
PshBI ATTAAT 1 cut(s) 810
Psp5II RGGWCCY 1 cut(s) 476
Psp6I CCWGG 2 cut(s) 774, 941
PspFI CCCAGC 2 cut(s) 132, 227
PspGI CCWGG 2 cut(s) 774, 941
PspN4I GGNNCC 2 cut(s) 477, 529
PspPI GGNCC 3 cut(s) 251, 476, 528
PspPPI RGGWCCY 1 cut(s) 476
PteI GCGCGC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 3 cut(s) 115, 572, 724
RsaNI GTAC 3 cut(s) 114, 571, 723
RseI CAYNNNNRTG 2 cut(s) 665, 967
SaqAI TTAA 7 cut(s) 485, 543, 810, 840, 909, 998, 1026
SatI GCNGC 5 cut(s) 5, 137, 355, 461, 698
Sau3AI GATC 2 cut(s) 929, 1163
Sau96I GGNCC 3 cut(s) 251, 476, 528
ScrFI CCNGG 3 cut(s) 255, 776, 943
SduI GDGCHC 2 cut(s) 338, 1047
SfaNI GCATC 2 cut(s) 605, 940
SgsI GGCGCGCC 1 cut(s) 7
SinI GGWCC 2 cut(s) 476, 528
SmiMI CAYNNNNRTG 2 cut(s) 665, 967
SmlI CTYRAG 2 cut(s) 26, 908
SmoI CTYRAG 2 cut(s) 26, 908
Sse9I AATT 9 cut(s) 151, 171, 246, 579, 836, 883, 989, 995, 1068
SsiI CCGC 5 cut(s) 4, 137, 277, 675, 772
SspI AATATT 2 cut(s) 391, 1051
SspMI CTAG 4 cut(s) 128, 420, 480, 1014
StyD4I CCNGG 3 cut(s) 253, 774, 941
TaaI ACNGT 1 cut(s) 500
TaiI ACGT 1 cut(s) 771
TaqI TCGA 1 cut(s) 221
TasI AATT 9 cut(s) 151, 171, 246, 579, 836, 883, 989, 995, 1068
TauI GCSGC 2 cut(s) 7, 139
TfiI GAWTC 2 cut(s) 223, 904
Tru1I TTAA 7 cut(s) 485, 543, 810, 840, 909, 998, 1026
Tru9I TTAA 7 cut(s) 485, 543, 810, 840, 909, 998, 1026
TseI GCWGC 3 cut(s) 354, 460, 697
TspDTI ATGAA 2 cut(s) 860, 1002
TspGWI ACGGA 5 cut(s) 97, 101, 133, 145, 262
Vha464I CTTAAG 1 cut(s) 908
VpaK11BI GGWCC 2 cut(s) 476, 528
VspI ATTAAT 1 cut(s) 810
XapI RAATTY 4 cut(s) 836, 883, 989, 1068
XceI RCATGY 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 1071
XspI CTAG 4 cut(s) 128, 420, 480, 1014
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.