RLG00000027495

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014410
Physical Location & Seq
Forward (+)
12162693 .. 12165739
3047 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027495

Sequence Viewer

Length: 1251 bp
ATGCGGCGCGCCATAGACGCCCACCTCAAGACCATGCGTCTCTTTCCTCCCCTCTCTTCCTTCTCCTCTTCTCCTCCTCCTCCTTCTTCTTCCACGCCTCTCTTCTCCGTCCGTACATTTCTTACCCTAGCCCAGCCGCGCTCCGTTTCCAATTCCGTCTCCAAAACCCTAATTTTCCACTCAATCGCCACTCCTTCCTACACTTCTTCTTCTTCCTCATCGATTCCCAGCCTCAGAAGCCACAGACTTGGGCCGACTATTGGGCTCTTTGCCGTGCGGTGCGTTTCGACGGCCTCGCCGGAGAGCAGTTTGGACTGGAACGAGCCGGTGTCGTGCTCCGAGGTCGGCGACGGCAGCAAAGGAAGTGTTGATAAGGATGATAAGCCCAATATTCCGGTCAGAGCTTACTTCTTTTCCACTAGTGTGGATTTGAGAAGCTTGGTGGAGCAGAACAGGCTGAATTTCATCCCTCCTACGTCGCGAATGACCAATTATGTTGTGTTTAAATTCGGGGAGCTTACCAGGTCTGACAGTGCTTCGGGTCCTAGTTTAAGTGGGAGCGACTGTTGCTACATGGTAGTTTTTCAATATGGGTCCATTGTCTTGTTTAATGTTCGTGAACATGAGATTGATGGGTACTTGAAAATTGTGGAAAAACATGCATCTGGATTGCTACCTGAAATGAGAAAGGATGAGTATGAGGTGAGAGAGAAACCAGATTTAGACACATGGATGGAAGGCGGATTGGATTACATAATGTTGCAGCACTTGAATACTGATGGTATTCGTACCATTGGTAGTGTTCTTGGTCAAAGTATTGCTCTGGATTACTACGTCCGCCAGGTTGATGGAATGGTTGCAGAGTTTACTGACATTAATCGTGAAATGGAAACTACTGGAAAATTTAAGATGAAGAGGAAGAAACTATTTCAGCTGGTGGGGAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTTGGACTTTTTGAGAGATCAGACATTGCCTGGAAGGATGCCAAATATGCTCAGATATGGGAATACCTCAGGGATGAATTTGAATTAACTCAGAGATTTGCTAGTCTTGACTTTAAGTTGAAGTTTGTGGAGCACAATATTCGCTTTCTTCAGGAAATTCTTCAAAACCGGAAATCAGATTTTTTGGAGTGGCTCATCATTGCACTAATAGGTGCTGAGATTATGCTCTCTCTTTATGACATTCTCCACAGATCAGCACTTGCTTCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

417

Amino Acids

46.95

Weight (kDa)

6.73

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 192 - 362 9.7e-35 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13610
fragaria_vesca FvH4_7g20360
malus_domestica MD01G1113300.v1.1 MD07G1180700.v1.1
prunus_persica Prupe.2G220000_v2.0.a1
pyrus_communis pycom01g14160 pycom07g17400
rosa_chinensis RchiOBHm_Chr1g0364321
rosa_laevigata RLG00000027495
rosa_multiflora Rmu_sc0007470.1_g000002
rosa_roxburghii Rroxscaffold_4G00292430
rosa_rugosa Rorug01G0315400
rosa_samantha Rh1AG323100 Rh1BG285700 Rh1CG302400 Rh1DG317200
rosa_wichuraiana Rw1G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 9, 139, 481
AciI CCGC 5 cut(s) 4, 137, 277, 741, 838
AcsI RAATTY 6 cut(s) 460, 506, 902, 949, 1055, 1134
AcuI CTGAAG 1 cut(s) 1112
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 3 cut(s) 115, 638, 790
AfiI CCNNNNNNNGG 1 cut(s) 260
AflII CTTAAG 1 cut(s) 974
AgsI TTSAA 6 cut(s) 587, 643, 772, 1061, 1099, 1142
AhdI GACNNNNNGTC 1 cut(s) 36
AhlI ACTAGT 1 cut(s) 419
AjnI CCWGG 3 cut(s) 521, 840, 1007
AleI CACNNNNGTG 1 cut(s) 422
AluBI AGCT 5 cut(s) 404, 438, 517, 934, 979
AluI AGCT 5 cut(s) 404, 438, 517, 934, 979
Alw21I GWGCWC 2 cut(s) 338, 1113
Alw26I GTCTC 2 cut(s) 44, 163
AoxI GGCC 2 cut(s) 251, 291
ApeKI GCWGC 2 cut(s) 354, 763
ApoI RAATTY 6 cut(s) 460, 506, 902, 949, 1055, 1134
AscI GGCGCGCC 1 cut(s) 7
AseI ATTAAT 1 cut(s) 876
Asp700I GAANNNNTTC 1 cut(s) 1137
AspLEI GCGC 3 cut(s) 9, 11, 141
AspS9I GGNCC 3 cut(s) 251, 542, 594
AsuHPI GGTGA 1 cut(s) 715
AvaII GGWCC 2 cut(s) 542, 594
AxyI CCTNAGG 1 cut(s) 1046
BanII GRGCYC 1 cut(s) 267
Bbv12I GWGCWC 2 cut(s) 338, 1113
BbvI GCAGC 2 cut(s) 366, 775
BccI CCATC 4 cut(s) 626, 727, 773, 842
BceAI ACGGC 3 cut(s) 257, 306, 367
BcgI CGANNNNNNTGC 2 cut(s) 1100, 1134
BciT130I CCWGG 3 cut(s) 523, 842, 1009
BcoDI GTCTC 2 cut(s) 44, 163
BcuI ACTAGT 1 cut(s) 419
BfaI CTAG 4 cut(s) 128, 420, 546, 1080
BfrI CTTAAG 1 cut(s) 974
BisI GCNGC 4 cut(s) 5, 137, 355, 764
BlsI GCNGC 4 cut(s) 6, 138, 356, 765
Bme1390I CCNGG 3 cut(s) 523, 842, 1009
Bme18I GGWCC 2 cut(s) 542, 594
BmeRI GACNNNNNGTC 1 cut(s) 36
BmgT120I GGNCC 3 cut(s) 251, 542, 594
BmiI GGNNCC 2 cut(s) 543, 595
BmrFI CCNGG 3 cut(s) 523, 842, 1009
BmsI GCATC 2 cut(s) 671, 1006
BpuEI CTTGAG 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 221
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 339
BsaWI WCCGGW 2 cut(s) 394, 1146
BsaXI ACNNNNNCTCC 2 cut(s) 550, 580
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 320, 901
Bse21I CCTNAGG 1 cut(s) 1046
Bse3DI GCAATG 2 cut(s) 1002, 1176
BseBI CCWGG 3 cut(s) 523, 842, 1009
BseCI ATCGAT 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 339
BseGI GGATG 6 cut(s) 382, 465, 697, 738, 1021, 1057
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 2 cut(s) 1002, 1176
BseMII CTCAG 5 cut(s) 247, 1043, 1060, 1082, 1185
BseNI ACTGG 2 cut(s) 320, 901
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 4 cut(s) 55, 63, 66, 69
BseXI GCAGC 2 cut(s) 366, 775
BseYI CCCAGC 2 cut(s) 132, 227
Bsh1236I CGCG 3 cut(s) 9, 139, 481
BshFI GGCC 2 cut(s) 253, 293
BshVI ATCGAT 1 cut(s) 221
BsiHKAI GWGCWC 2 cut(s) 338, 1113
BsiSI CCGG 4 cut(s) 299, 326, 395, 1147
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 2 cut(s) 44, 163
BsmBI CGTCTC 2 cut(s) 44, 163
BsnI GGCC 2 cut(s) 253, 293
Bsp1286I GDGCHC 3 cut(s) 267, 338, 1113
Bsp143I GATC 2 cut(s) 995, 1229
Bsp68I TCGCGA 1 cut(s) 481
BspACI CCGC 5 cut(s) 4, 137, 277, 741, 838
BspANI GGCC 2 cut(s) 253, 293
BspCNI CTCAG 5 cut(s) 246, 1042, 1059, 1081, 1186
BspDI ATCGAT 1 cut(s) 221
BspFNI CGCG 3 cut(s) 9, 139, 481
BspLI GGNNCC 2 cut(s) 543, 595
BspTI CTTAAG 1 cut(s) 974
BsrDI GCAATG 2 cut(s) 1002, 1176
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 2 cut(s) 320, 901
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 339
BssHII GCGCGC 1 cut(s) 7
BssMI GATC 2 cut(s) 995, 1229
BssNI GRCGYC 1 cut(s) 18
Bst2UI CCWGG 3 cut(s) 523, 842, 1009
Bst4CI ACNGT 2 cut(s) 533, 566
Bst6I CTCTTC 4 cut(s) 61, 73, 107, 908
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 974
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 5 cut(s) 233, 1029, 1046, 1068, 1194
BstF5I GGATG 6 cut(s) 382, 465, 697, 738, 1021, 1057
BstFNI CGCG 3 cut(s) 9, 139, 481
BstHHI GCGC 3 cut(s) 9, 11, 141
BstKTI GATC 2 cut(s) 998, 1232
BstMAI GTCTC 2 cut(s) 44, 163
BstMBI GATC 2 cut(s) 995, 1229
BstMWI GCNNNNNNNGC 6 cut(s) 17, 237, 354, 454, 567, 1025
BstNI CCWGG 3 cut(s) 523, 842, 1009
BstNSI RCATGY 1 cut(s) 662
BstSCI CCNGG 3 cut(s) 521, 840, 1007
BstUI CGCG 3 cut(s) 9, 139, 481
BstV1I GCAGC 2 cut(s) 366, 775
BstXI CCANNNNNNTGG 3 cut(s) 248, 424, 848
Bsu15I ATCGAT 1 cut(s) 221
Bsu36I CCTNAGG 1 cut(s) 1046
BsuRI GGCC 2 cut(s) 253, 293
BsuTUI ATCGAT 1 cut(s) 221
BtsCI GGATG 6 cut(s) 382, 465, 697, 738, 1021, 1057
BtsIMutI CAGTG 1 cut(s) 538
BtuMI TCGCGA 1 cut(s) 481
Cac8I GCNNGC 1 cut(s) 9
CfoI GCGC 3 cut(s) 9, 11, 141
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 3 cut(s) 251, 542, 594
ClaI ATCGAT 1 cut(s) 221
CseI GACGC 2 cut(s) 26, 26
CsiI ACCWGGT 1 cut(s) 521
Csp6I GTAC 3 cut(s) 114, 637, 789
CviAII CATG 5 cut(s) 34, 574, 623, 659, 729
CviQI GTAC 3 cut(s) 114, 637, 789
DdeI CTNAG 5 cut(s) 233, 1029, 1046, 1068, 1194
DpnI GATC 2 cut(s) 997, 1231
DpnII GATC 2 cut(s) 995, 1229
DraI TTTAAA 1 cut(s) 505
DriI GACNNNNNGTC 1 cut(s) 36
Eam1104I CTCTTC 4 cut(s) 61, 73, 107, 908
Eam1105I GACNNNNNGTC 1 cut(s) 36
EarI CTCTTC 4 cut(s) 61, 73, 107, 908
EciI GGCGGA 2 cut(s) 756, 827
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 2 cut(s) 542, 594
Eco57I CTGAAG 1 cut(s) 1112
Eco81I CCTNAGG 1 cut(s) 1046
EcoO109I RGGNCCY 1 cut(s) 542
EcoRII CCWGG 3 cut(s) 521, 840, 1007
EcoT22I ATGCAT 1 cut(s) 664
EcoT38I GRGCYC 1 cut(s) 267
Esp3I CGTCTC 2 cut(s) 44, 163
FaeI CATG 5 cut(s) 37, 577, 626, 662, 732
FatI CATG 5 cut(s) 33, 573, 622, 658, 728
Fnu4HI GCNGC 4 cut(s) 5, 137, 355, 764
FokI GGATG 6 cut(s) 389, 452, 704, 745, 1028, 1064
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 4 cut(s) 5, 137, 355, 764
FspBI CTAG 4 cut(s) 128, 420, 546, 1080
GlaI GCGC 3 cut(s) 8, 10, 140
GluI GCNGC 4 cut(s) 5, 137, 355, 764
GsaI CCCAGC 2 cut(s) 136, 231
HaeIII GGCC 2 cut(s) 253, 293
HapII CCGG 4 cut(s) 299, 326, 395, 1147
HgaI GACGC 2 cut(s) 26, 26
HhaI GCGC 3 cut(s) 9, 11, 141
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 5 cut(s) 37, 577, 626, 662, 732
Hin6I GCGC 3 cut(s) 7, 9, 139
HinP1I GCGC 3 cut(s) 7, 9, 139
HindIII AAGCTT 2 cut(s) 436, 977
HinfI GANTC 2 cut(s) 223, 970
HpaII CCGG 4 cut(s) 299, 326, 395, 1147
HphI GGTGA 1 cut(s) 715
Hpy166II GTNNAC 2 cut(s) 620, 867
Hpy188I TCNGA 8 cut(s) 236, 340, 401, 529, 1000, 1032, 1071, 1156
Hpy188III TCNNGA 8 cut(s) 28, 480, 617, 666, 824, 881, 1085, 1130
Hpy8I GTNNAC 2 cut(s) 620, 867
Hpy99I CGWCG 3 cut(s) 292, 353, 481
HpyAV CCTTC 6 cut(s) 70, 93, 204, 731, 937, 1006
HpyCH4III ACNGT 2 cut(s) 533, 566
HpyCH4IV ACGT 2 cut(s) 476, 834
HpyCH4V TGCA 4 cut(s) 662, 763, 860, 1181
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 237, 354, 454, 567, 1025
HpyF3I CTNAG 5 cut(s) 233, 1029, 1046, 1068, 1194
HpySE526I ACGT 2 cut(s) 476, 834
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 5 cut(s) 37, 577, 626, 662, 732
HspAI GCGC 3 cut(s) 7, 9, 139
Kzo9I GATC 2 cut(s) 995, 1229
LmnI GCTCC 6 cut(s) 146, 341, 445, 514, 558, 1108
Lsp1109I GCAGC 2 cut(s) 366, 775
LweI GCATC 2 cut(s) 671, 1006
MabI ACCWGGT 1 cut(s) 521
MaeI CTAG 4 cut(s) 128, 420, 546, 1080
MaeII ACGT 2 cut(s) 476, 834
MalI GATC 2 cut(s) 997, 1231
MboI GATC 2 cut(s) 995, 1229
MhlI GDGCHC 3 cut(s) 267, 338, 1113
Mph1103I ATGCAT 1 cut(s) 664
MroXI GAANNNNTTC 1 cut(s) 1137
MseI TTAA 8 cut(s) 504, 551, 609, 876, 906, 975, 1064, 1092
MslI CAYNNNNRTG 2 cut(s) 422, 731
MspA1I CMGCKG 1 cut(s) 934
MspCI CTTAAG 1 cut(s) 974
MspI CCGG 4 cut(s) 299, 326, 395, 1147
MspR9I CCNGG 3 cut(s) 523, 842, 1009
MvaI CCWGG 3 cut(s) 523, 842, 1009
MvnI CGCG 3 cut(s) 9, 139, 481
MwoI GCNNNNNNNGC 6 cut(s) 17, 237, 354, 454, 567, 1025
NdeII GATC 2 cut(s) 995, 1229
NlaIII CATG 5 cut(s) 37, 577, 626, 662, 732
NlaIV GGNNCC 2 cut(s) 543, 595
NruI TCGCGA 1 cut(s) 481
NsiI ATGCAT 1 cut(s) 664
NspI RCATGY 1 cut(s) 662
OliI CACNNNNGTG 1 cut(s) 422
PalAI GGCGCGCC 1 cut(s) 7
PauI GCGCGC 1 cut(s) 7
PdmI GAANNNNTTC 1 cut(s) 1137
PfeI GAWTC 2 cut(s) 223, 970
PkrI GCNGC 4 cut(s) 6, 138, 356, 765
PpuMI RGGWCCY 1 cut(s) 542
PshBI ATTAAT 1 cut(s) 876
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 3 cut(s) 521, 840, 1007
PspFI CCCAGC 2 cut(s) 132, 227
PspGI CCWGG 3 cut(s) 521, 840, 1007
PspN4I GGNNCC 2 cut(s) 543, 595
PspPI GGNCC 3 cut(s) 251, 542, 594
PspPPI RGGWCCY 1 cut(s) 542
PteI GCGCGC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 934
RruI TCGCGA 1 cut(s) 481
RsaI GTAC 3 cut(s) 115, 638, 790
RsaNI GTAC 3 cut(s) 114, 637, 789
RseI CAYNNNNRTG 2 cut(s) 422, 731
SaqAI TTAA 8 cut(s) 504, 551, 609, 876, 906, 975, 1064, 1092
SatI GCNGC 4 cut(s) 5, 137, 355, 764
Sau3AI GATC 2 cut(s) 995, 1229
Sau96I GGNCC 3 cut(s) 251, 542, 594
ScrFI CCNGG 3 cut(s) 523, 842, 1009
SduI GDGCHC 3 cut(s) 267, 338, 1113
SexAI ACCWGGT 1 cut(s) 521
SfaNI GCATC 2 cut(s) 671, 1006
SgsI GGCGCGCC 1 cut(s) 7
SinI GGWCC 2 cut(s) 542, 594
SmiMI CAYNNNNRTG 2 cut(s) 422, 731
SmlI CTYRAG 2 cut(s) 26, 974
SmoI CTYRAG 2 cut(s) 26, 974
SpeI ACTAGT 1 cut(s) 419
SsiI CCGC 5 cut(s) 4, 137, 277, 741, 838
SspI AATATT 2 cut(s) 391, 1117
SspMI CTAG 4 cut(s) 128, 420, 546, 1080
StyD4I CCNGG 3 cut(s) 521, 840, 1007
TaaI ACNGT 2 cut(s) 533, 566
TaiI ACGT 2 cut(s) 479, 837
TaqI TCGA 2 cut(s) 221, 287
TauI GCSGC 2 cut(s) 7, 139
TfiI GAWTC 2 cut(s) 223, 970
Tru1I TTAA 8 cut(s) 504, 551, 609, 876, 906, 975, 1064, 1092
Tru9I TTAA 8 cut(s) 504, 551, 609, 876, 906, 975, 1064, 1092
TscAI CASTG 1 cut(s) 538
TseI GCWGC 2 cut(s) 354, 763
TspDTI ATGAA 3 cut(s) 454, 926, 1068
TspGWI ACGGA 4 cut(s) 97, 101, 133, 145
TspRI CASTG 1 cut(s) 538
Vha464I CTTAAG 1 cut(s) 974
VpaK11BI GGWCC 2 cut(s) 542, 594
VspI ATTAAT 1 cut(s) 876
XapI RAATTY 6 cut(s) 460, 506, 902, 949, 1055, 1134
XceI RCATGY 1 cut(s) 662
XmnI GAANNNNTTC 1 cut(s) 1137
XspI CTAG 4 cut(s) 128, 420, 546, 1080
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.