Prupe.2G220000_v2.0.a1

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0014410
Physical Location & Seq
Reverse (-)
24855085 .. 24858510
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G220000.1

Sequence Viewer

Length: 1227 bp
ATGCGGCGTGCCATAGACGCCCACCTCAAGGCCACCCGTCTCTTTCCCTCTCTCTCTTCTCTTTCTTCGTCTTCATCTTCTTCGCCTACGCCTCTCCTCTCCGTCCGTACAATCCTAACCTTACCCCATCCTCAAAATCCTTCCATTTCCAAAACCCTAAACCTCCACTCAATCACCACGTCTTGGTGTTCCTCGATTCCAGGTCTCAGAAGCCAACCCCAAATCGGCTCCAATTTCAACGTCCTTGTCGTACGCTGCGTTTCATCTGTCCCGCATGCAGACACATTGGAGTGGAACGAGCCGGTCTCGTGCTCGGAGGTCGGCGATGGCCACAAAGCGACGGTGGACGAAAATGCTAAGCCCTCTATCCCTGTTAGAGCCTACTTCTTCTCTACCAGTGTGGATTTGAGAAGCTTAGTGGAACAGAACAGGCTGAATTTTATCCCACCAACATCGCGTATGACTAATTACGTTGTGTTTAGGTTCGGCGAGCCGTCCGATTCTAACAATGGTTTGGGTCTTAGTTTAAGTGGGAGTGACTGTTGCTACATGGTAGTTTTTCAGTATGGCTCCATTGTCTTGTTTAATGTTCGTGAACACGAGATCGATGGGTATTTGAAAATCGTGGAAAAACATGCATCAGGATTGCTACCTGAAATGAGAAAGGATGAGTATGAGGTGAGGGAGAAGCCAGCTTTGCACACTTGGATGGAAGGTGGATTGGATTACATAATGTTGCAGTACTTGAATGTTGATGGAATCCGTACTATTGGTAGTGTTCTTGGTCAAAGTATTGCTCTTGATTACTATGTGCGCCAGGTTGATGGAATGGTTGCAGAATTTACTGACATTAATCGTGAAATGGAAACTACGGGAAAGTTTAAAATGAAGAGGAAAAAACTATTTCAGTTAGTGGGAAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTCGGACTTTTTGAGAGATCAGACATTGCCTGGAAGGATGCCAAATATGCCCAGATATGGGAGTATCTCAGAGATGAATTTGAGTTAACTCAGAGATTTGCAAGTCTTGATTTTAAGTTGAAGTTTGTGGAGCACAACATTCGCTTTCTTCAGGAAATTCTTCAAAATAGGAAGTCCGATTTTTTGGAATGGCTCATCATTGCGCTGATAGGTGCCGAAATTCTTCTCTCTCTGTATGACATTGTCCACCGATCGGCACTTGCTACGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

409

Amino Acids

46.17

Weight (kDa)

6.57

Isoelectric Point (pI)

36.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13610
fragaria_vesca FvH4_7g20360
malus_domestica MD01G1113300.v1.1 MD07G1180700.v1.1
prunus_persica Prupe.2G220000_v2.0.a1
pyrus_communis pycom01g14160 pycom07g17400
rosa_chinensis RchiOBHm_Chr1g0364321
rosa_laevigata RLG00000027495
rosa_multiflora Rmu_sc0007470.1_g000002
rosa_roxburghii Rroxscaffold_4G00292430
rosa_rugosa Rorug01G0315400
rosa_samantha Rh1AG323100 Rh1BG285700 Rh1CG302400 Rh1DG317200
rosa_wichuraiana Rw1G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1166
AccB7I CCANNNNNTGG 1 cut(s) 183
AccII CGCG 1 cut(s) 457
AciI CCGC 2 cut(s) 4, 272
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 6 cut(s) 436, 839, 925, 1031, 1110, 1173
AcuI CTGAAG 1 cut(s) 1088
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 4 cut(s) 109, 252, 743, 766
AfiI CCNNNNNNNGG 6 cut(s) 28, 183, 224, 1011, 1012, 1207
AflII CTTAAG 1 cut(s) 950
AgsI TTSAA 5 cut(s) 238, 619, 748, 1075, 1118
AjiI CACGTC 1 cut(s) 180
AjnI CCWGG 3 cut(s) 199, 816, 983
AluBI AGCT 3 cut(s) 414, 695, 955
AluI AGCT 3 cut(s) 414, 695, 955
Alw21I GWGCWC 2 cut(s) 314, 1089
Alw26I GTCTC 3 cut(s) 44, 209, 310
AoxI GGCC 2 cut(s) 30, 328
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 6 cut(s) 436, 839, 925, 1031, 1110, 1173
AseI ATTAAT 1 cut(s) 852
Asp700I GAANNNNTTC 2 cut(s) 1113, 1176
AspLEI GCGC 2 cut(s) 816, 1159
AsuHPI GGTGA 2 cut(s) 166, 691
BalI TGGCCA 1 cut(s) 330
BanI GGYRCC 1 cut(s) 1166
BauI CACGAG 2 cut(s) 307, 599
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 2 cut(s) 314, 1089
BbvI GCAGC 1 cut(s) 242
BccI CCATC 6 cut(s) 135, 320, 602, 703, 749, 818
BceAI ACGGC 1 cut(s) 478
BcgI CGANNNNNNTGC 2 cut(s) 1076, 1110
BciT130I CCWGG 3 cut(s) 201, 818, 985
BcoDI GTCTC 3 cut(s) 44, 209, 310
BfrI CTTAAG 1 cut(s) 950
BisI GCNGC 2 cut(s) 5, 256
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 2 cut(s) 6, 257
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 3 cut(s) 201, 818, 985
BmgBI CACGTC 1 cut(s) 180
BmiI GGNNCC 3 cut(s) 229, 571, 1168
BmrFI CCNGG 3 cut(s) 201, 818, 985
BmsI GCATC 2 cut(s) 647, 982
BpiI GAAGAC 1 cut(s) 63
BplI GAGNNNNNCTC 2 cut(s) 290, 322
Bpu1102I GCTNAGC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 209, 310
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 183, 224, 1011, 1012, 1207
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 396
Bse3DI GCAATG 2 cut(s) 978, 1152
BseBI CCWGG 3 cut(s) 201, 818, 985
BseCI ATCGAT 1 cut(s) 606
BseGI GGATG 4 cut(s) 127, 673, 714, 997
BseLI CCNNNNNNNGG 6 cut(s) 28, 183, 224, 1011, 1012, 1207
BseMI GCAATG 2 cut(s) 978, 1152
BseMII CTCAG 3 cut(s) 220, 1036, 1058
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 457
Bsh1285I CGRYCG 1 cut(s) 1208
BshFI GGCC 2 cut(s) 32, 330
BshNI GGYRCC 1 cut(s) 1166
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 1208
BsiHKAI GWGCWC 2 cut(s) 314, 1089
BsiSI CCGG 1 cut(s) 302
BsiWI CGTACG 1 cut(s) 250
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 6 cut(s) 28, 183, 224, 1011, 1012, 1207
BsmAI GTCTC 3 cut(s) 44, 209, 310
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 2 cut(s) 32, 330
Bso31I GGTCTC 2 cut(s) 209, 310
Bsp1286I GDGCHC 2 cut(s) 314, 1089
Bsp143I GATC 3 cut(s) 603, 971, 1205
Bsp1720I GCTNAGC 1 cut(s) 357
BspACI CCGC 2 cut(s) 4, 272
BspANI GGCC 2 cut(s) 32, 330
BspCNI CTCAG 3 cut(s) 219, 1035, 1057
BspDI ATCGAT 1 cut(s) 606
BspFNI CGCG 1 cut(s) 457
BspLI GGNNCC 3 cut(s) 229, 571, 1168
BspT107I GGYRCC 1 cut(s) 1166
BspTI CTTAAG 1 cut(s) 950
BspTNI GGTCTC 2 cut(s) 209, 310
BsrDI GCAATG 2 cut(s) 978, 1152
BsrFI RCCGGY 1 cut(s) 301
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 1 cut(s) 301
BssMI GATC 3 cut(s) 603, 971, 1205
BssNI GRCGYC 1 cut(s) 18
BssSI CACGAG 2 cut(s) 307, 599
Bst2BI CACGAG 2 cut(s) 307, 599
Bst2UI CCWGG 3 cut(s) 201, 818, 985
Bst4CI ACNGT 2 cut(s) 343, 542
Bst6I CTCTTC 2 cut(s) 61, 884
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 950
BstC8I GCNNGC 4 cut(s) 9, 276, 491, 693
BstDEI CTNAG 6 cut(s) 206, 357, 415, 521, 1022, 1044
BstF5I GGATG 4 cut(s) 127, 673, 714, 997
BstFNI CGCG 1 cut(s) 457
BstHHI GCGC 2 cut(s) 816, 1159
BstKTI GATC 3 cut(s) 606, 974, 1208
BstMAI GTCTC 3 cut(s) 44, 209, 310
BstMBI GATC 3 cut(s) 603, 971, 1205
BstMCI CGRYCG 1 cut(s) 1208
BstMWI GCNNNNNNNGC 3 cut(s) 17, 697, 1001
BstNI CCWGG 3 cut(s) 201, 818, 985
BstNSI RCATGY 2 cut(s) 278, 638
BstSCI CCNGG 3 cut(s) 199, 816, 983
BstUI CGCG 1 cut(s) 457
BstV1I GCAGC 1 cut(s) 242
BstV2I GAAGAC 1 cut(s) 63
BstXI CCANNNNNNTGG 1 cut(s) 824
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 2 cut(s) 32, 330
BsuTUI ATCGAT 1 cut(s) 606
BtgZI GCGATG 2 cut(s) 339, 438
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 4 cut(s) 127, 673, 714, 997
BtsIMutI CAGTG 1 cut(s) 403
Cac8I GCNNGC 4 cut(s) 9, 276, 491, 693
CfoI GCGC 2 cut(s) 816, 1159
Cfr10I RCCGGY 1 cut(s) 301
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 1 cut(s) 26
Csp6I GTAC 4 cut(s) 108, 251, 742, 765
CviAII CATG 3 cut(s) 275, 550, 635
CviQI GTAC 4 cut(s) 108, 251, 742, 765
DdeI CTNAG 6 cut(s) 206, 357, 415, 521, 1022, 1044
DpnI GATC 3 cut(s) 605, 973, 1207
DpnII GATC 3 cut(s) 603, 971, 1205
DraI TTTAAA 1 cut(s) 883
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 61, 884
EarI CTCTTC 2 cut(s) 61, 884
Eco31I GGTCTC 2 cut(s) 209, 310
Eco57I CTGAAG 1 cut(s) 1088
EcoRII CCWGG 3 cut(s) 199, 816, 983
EcoT22I ATGCAT 1 cut(s) 640
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 3 cut(s) 278, 553, 638
FaqI GGGAC 1 cut(s) 254
FatI CATG 3 cut(s) 274, 549, 634
FauI CCCGC 1 cut(s) 279
Fnu4HI GCNGC 2 cut(s) 5, 256
FokI GGATG 4 cut(s) 114, 680, 721, 1004
Fsp4HI GCNGC 2 cut(s) 5, 256
GlaI GCGC 2 cut(s) 815, 1158
GluI GCNGC 2 cut(s) 5, 256
HaeIII GGCC 2 cut(s) 32, 330
HapII CCGG 1 cut(s) 302
HgaI GACGC 1 cut(s) 26
HhaI GCGC 2 cut(s) 816, 1159
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 3 cut(s) 278, 553, 638
Hin6I GCGC 2 cut(s) 814, 1157
HinP1I GCGC 2 cut(s) 814, 1157
HincII GTYRAC 1 cut(s) 1041
HindII GTYRAC 1 cut(s) 1041
HindIII AAGCTT 1 cut(s) 412
HinfI GANTC 4 cut(s) 196, 500, 759, 946
HpaI GTTAAC 1 cut(s) 1041
HpaII CCGG 1 cut(s) 302
HphI GGTGA 2 cut(s) 166, 691
Hpy166II GTNNAC 4 cut(s) 346, 596, 1041, 1201
Hpy188I TCNGA 8 cut(s) 209, 316, 499, 959, 976, 1025, 1047, 1132
Hpy188III TCNNGA 6 cut(s) 593, 642, 800, 857, 1061, 1106
Hpy8I GTNNAC 4 cut(s) 346, 596, 1041, 1201
Hpy99I CGWCG 1 cut(s) 343
HpyAV CCTTC 3 cut(s) 150, 707, 982
HpyCH4III ACNGT 2 cut(s) 343, 542
HpyCH4IV ACGT 3 cut(s) 179, 240, 471
HpyCH4V TGCA 6 cut(s) 278, 638, 700, 739, 836, 1055
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 697, 1001
HpyF3I CTNAG 6 cut(s) 206, 357, 415, 521, 1022, 1044
HpySE526I ACGT 3 cut(s) 179, 240, 471
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 3 cut(s) 278, 553, 638
HspAI GCGC 2 cut(s) 814, 1157
KspAI GTTAAC 1 cut(s) 1041
Kzo9I GATC 3 cut(s) 603, 971, 1205
LmnI GCTCC 3 cut(s) 233, 575, 1084
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 2 cut(s) 647, 982
MaeII ACGT 3 cut(s) 179, 240, 471
MaeIII GTNAC 1 cut(s) 536
MalI GATC 3 cut(s) 605, 973, 1207
MboI GATC 3 cut(s) 603, 971, 1205
MhlI GDGCHC 2 cut(s) 314, 1089
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 8 cut(s) 232, 436, 466, 839, 925, 1031, 1110, 1173
MluNI TGGCCA 1 cut(s) 330
Mox20I TGGCCA 1 cut(s) 330
Mph1103I ATGCAT 1 cut(s) 640
MroXI GAANNNNTTC 2 cut(s) 1113, 1176
MscI TGGCCA 1 cut(s) 330
MseI TTAA 7 cut(s) 527, 585, 852, 882, 951, 1040, 1068
MslI CAYNNNNRTG 2 cut(s) 289, 707
Msp20I TGGCCA 1 cut(s) 330
MspCI CTTAAG 1 cut(s) 950
MspI CCGG 1 cut(s) 302
MspR9I CCNGG 3 cut(s) 201, 818, 985
MvaI CCWGG 3 cut(s) 201, 818, 985
MvnI CGCG 1 cut(s) 457
MwoI GCNNNNNNNGC 3 cut(s) 17, 697, 1001
NdeII GATC 3 cut(s) 603, 971, 1205
NlaIII CATG 3 cut(s) 278, 553, 638
NlaIV GGNNCC 3 cut(s) 229, 571, 1168
NmuCI GTSAC 1 cut(s) 536
NsiI ATGCAT 1 cut(s) 640
NspI RCATGY 2 cut(s) 278, 638
PaeI GCATGC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 255
PdmI GAANNNNTTC 2 cut(s) 1113, 1176
PfeI GAWTC 4 cut(s) 196, 500, 759, 946
Pfl23II CGTACG 1 cut(s) 250
PflFI GACNNNGTC 1 cut(s) 1196
PflMI CCANNNNNTGG 1 cut(s) 183
PkrI GCNGC 2 cut(s) 6, 257
Ple19I CGATCG 1 cut(s) 1208
PshBI ATTAAT 1 cut(s) 852
Psp6I CCWGG 3 cut(s) 199, 816, 983
PspGI CCWGG 3 cut(s) 199, 816, 983
PspLI CGTACG 1 cut(s) 250
PspN4I GGNNCC 3 cut(s) 229, 571, 1168
PsyI GACNNNGTC 1 cut(s) 1196
PvuI CGATCG 1 cut(s) 1208
RsaI GTAC 4 cut(s) 109, 252, 743, 766
RsaNI GTAC 4 cut(s) 108, 251, 742, 765
RseI CAYNNNNRTG 2 cut(s) 289, 707
SaqAI TTAA 7 cut(s) 527, 585, 852, 882, 951, 1040, 1068
SatI GCNGC 2 cut(s) 5, 256
Sau3AI GATC 3 cut(s) 603, 971, 1205
ScaI AGTACT 1 cut(s) 743
ScrFI CCNGG 3 cut(s) 201, 818, 985
SduI GDGCHC 2 cut(s) 314, 1089
SfaNI GCATC 2 cut(s) 647, 982
SmiMI CAYNNNNRTG 2 cut(s) 289, 707
SmlI CTYRAG 2 cut(s) 26, 950
SmoI CTYRAG 2 cut(s) 26, 950
SphI GCATGC 1 cut(s) 278
Sse9I AATT 8 cut(s) 232, 436, 466, 839, 925, 1031, 1110, 1173
SsiI CCGC 2 cut(s) 4, 272
StyD4I CCNGG 3 cut(s) 199, 816, 983
TaaI ACNGT 2 cut(s) 343, 542
TaiI ACGT 3 cut(s) 182, 243, 474
TaqI TCGA 2 cut(s) 194, 606
TasI AATT 8 cut(s) 232, 436, 466, 839, 925, 1031, 1110, 1173
TatI WGTACW 1 cut(s) 741
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 4 cut(s) 196, 500, 759, 946
Tru1I TTAA 7 cut(s) 527, 585, 852, 882, 951, 1040, 1068
Tru9I TTAA 7 cut(s) 527, 585, 852, 882, 951, 1040, 1068
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 1 cut(s) 255
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 4 cut(s) 63, 252, 902, 1044
TspGWI ACGGA 3 cut(s) 91, 95, 752
TspRI CASTG 1 cut(s) 403
Tth111I GACNNNGTC 1 cut(s) 1196
Van91I CCANNNNNTGG 1 cut(s) 183
Vha464I CTTAAG 1 cut(s) 950
VspI ATTAAT 1 cut(s) 852
XapI RAATTY 6 cut(s) 436, 839, 925, 1031, 1110, 1173
XceI RCATGY 2 cut(s) 278, 638
XmnI GAANNNNTTC 2 cut(s) 1113, 1176
ZrmI AGTACT 1 cut(s) 743
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.