Rh1AG323100

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0014410
Physical Location & Seq
Reverse (-)
55696375 .. 55699757
3383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG323100.1

Sequence Viewer

Length: 1260 bp
ATGCGGCGCGCCATAGACGCTCACCTCAAGACCATGCGTCTCTTTCCTCCCCTCTCTTCCTTCTCCTCTTCTTCTCCTCCTTCTTCTTCTTCCACGCCTCTCTTCTCCGTCCGTACATTTCTGACCCTAGCCCAGCCGCGCTCCGTTTCCAATTCCGTCTCCAAAACCCTAATTTTCCACTCAATCGCTACTCCTTCCTACACTTCTTCTTCTTCTTCTTCCTCCTCATCGATTCCCAGCCTCAGAAGCCACAGACTTGGGCCGGCTATTGGGCTCTTTGCCGTGCGGTGCGTCTCGACGGCCTCGCCGGAGAGCAGTTTGGACTGGAACGAGCCGGTGTCGTGCTCCGAGGTCGGCGACGGCAGCAAAGGAAGTGTTGATAAGGATGATAAGCCCAATATTCCGGTCAGAGCTTACTTCTTTTCCACTAGTGTGGATTTGAGAAGCTTGGTGGAGCAGAACAGGCTGAATTTCATCCCTCCTACGTCGCGAATGACCAATTATGTTGTGTTTAAATTCGGGGAGCTTACCAGGTCTGACAATGCTTCGGGTCCTAGTTTAAGTGGGAGCGACTGTTGCTACATGGTAGTTTTTCAATATGGGTCCATTGTCTTGTTTAATGTTGGTGAACATGAGATTGATGGGTACTTGAAAATTGTGGAAAAACATGCATCTGGATTGCTACCTGAAATGAGAAAGGATGAGTATGAGGTGAGAGAGAAACCAGATTTAGACACATGGATGGAAGGCGGATTGGATTACATAATGTTGCAGCACTTGAATACTGATGGTATTCGTACCATTGGTAGTGTTCTTGGTCAAAGTATTGCTCTGGATTACTACGTCCGTCAGGTTGATGGAATGGTTGCAGAGTTTACTGACATTAATCGTGAAATGGAAACTACTGGAAAATTTAAGATGAAGAGGAAGAAACTATTTCAACTGGTGGGGAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTTGGACTTTTTGAGAGATCAGACATTGCCTGGAAGGATGCCAAATATGCTCAGATTTGGGAATACCTCAGGGATGAATTTGAATTAACTCAGAGATTTGCTAGTCTTGACTTTAAGTTGAAGTTTGTGGAGCACAATATTCGCTTTCTTCAGGAAATTCTTCAAAACCGGAAATCAGATTTTTTGGAGTGGCTCATCATTGCACTAATAGGTGCTGAGATTATGCTCTCTCTTTATGACATTCTCCACAGATCAGCACTCGCTTCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

419

Amino Acids

47.09

Weight (kDa)

6.48

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 195 - 365 1.4e-34 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13610
fragaria_vesca FvH4_7g20360
malus_domestica MD01G1113300.v1.1 MD07G1180700.v1.1
prunus_persica Prupe.2G220000_v2.0.a1
pyrus_communis pycom01g14160 pycom07g17400
rosa_chinensis RchiOBHm_Chr1g0364321
rosa_laevigata RLG00000027495
rosa_multiflora Rmu_sc0007470.1_g000002
rosa_roxburghii Rroxscaffold_4G00292430
rosa_rugosa Rorug01G0315400
rosa_samantha Rh1AG323100 Rh1BG285700 Rh1CG302400 Rh1DG317200
rosa_wichuraiana Rw1G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 9, 139, 490
AciI CCGC 4 cut(s) 4, 137, 286, 750
AcsI RAATTY 6 cut(s) 469, 515, 911, 958, 1064, 1143
AcuI CTGAAG 1 cut(s) 1121
AfaI GTAC 3 cut(s) 115, 647, 799
AfiI CCNNNNNNNGG 1 cut(s) 269
AflII CTTAAG 1 cut(s) 983
AgsI TTSAA 7 cut(s) 596, 652, 781, 941, 1070, 1108, 1151
AhdI GACNNNNNGTC 1 cut(s) 36
AhlI ACTAGT 1 cut(s) 428
AjnI CCWGG 2 cut(s) 530, 1016
AleI CACNNNNGTG 1 cut(s) 431
AluBI AGCT 4 cut(s) 413, 447, 526, 988
AluI AGCT 4 cut(s) 413, 447, 526, 988
Alw21I GWGCWC 2 cut(s) 347, 1122
Alw26I GTCTC 3 cut(s) 44, 163, 298
AoxI GGCC 2 cut(s) 260, 300
ApeKI GCWGC 2 cut(s) 363, 772
ApoI RAATTY 6 cut(s) 469, 515, 911, 958, 1064, 1143
AscI GGCGCGCC 1 cut(s) 7
AseI ATTAAT 1 cut(s) 885
Asp700I GAANNNNTTC 1 cut(s) 1146
AspLEI GCGC 3 cut(s) 9, 11, 141
AspS9I GGNCC 3 cut(s) 260, 551, 603
AsuHPI GGTGA 3 cut(s) 14, 638, 724
AvaII GGWCC 2 cut(s) 551, 603
AxyI CCTNAGG 1 cut(s) 1055
BanII GRGCYC 1 cut(s) 276
Bbv12I GWGCWC 2 cut(s) 347, 1122
BbvI GCAGC 2 cut(s) 375, 784
BccI CCATC 4 cut(s) 635, 736, 782, 851
BceAI ACGGC 3 cut(s) 266, 315, 376
BcgI CGANNNNNNTGC 2 cut(s) 1109, 1143
BciT130I CCWGG 2 cut(s) 532, 1018
BcoDI GTCTC 3 cut(s) 44, 163, 298
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 4 cut(s) 128, 429, 555, 1089
BfrI CTTAAG 1 cut(s) 983
BisI GCNGC 4 cut(s) 5, 137, 364, 773
BlsI GCNGC 4 cut(s) 6, 138, 365, 774
Bme1390I CCNGG 2 cut(s) 532, 1018
Bme18I GGWCC 2 cut(s) 551, 603
BmeRI GACNNNNNGTC 1 cut(s) 36
BmgT120I GGNCC 3 cut(s) 260, 551, 603
BmiI GGNNCC 2 cut(s) 552, 604
BmrFI CCNGG 2 cut(s) 532, 1018
BmsI GCATC 2 cut(s) 680, 1015
BpuEI CTTGAG 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 348
BsaWI WCCGGW 2 cut(s) 403, 1155
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse118I RCCGGY 2 cut(s) 262, 334
Bse1I ACTGG 3 cut(s) 329, 910, 948
Bse21I CCTNAGG 1 cut(s) 1055
Bse3DI GCAATG 2 cut(s) 1011, 1185
BseBI CCWGG 2 cut(s) 532, 1018
BseCI ATCGAT 1 cut(s) 230
BseDI CCNNGG 1 cut(s) 348
BseGI GGATG 6 cut(s) 391, 474, 706, 747, 1030, 1066
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 2 cut(s) 1011, 1185
BseMII CTCAG 5 cut(s) 256, 1052, 1069, 1091, 1194
BseNI ACTGG 3 cut(s) 329, 910, 948
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 3 cut(s) 55, 66, 214
BseXI GCAGC 2 cut(s) 375, 784
BseYI CCCAGC 2 cut(s) 132, 236
Bsh1236I CGCG 3 cut(s) 9, 139, 490
BshFI GGCC 2 cut(s) 262, 302
BshVI ATCGAT 1 cut(s) 230
BsiHKAI GWGCWC 2 cut(s) 347, 1122
BsiSI CCGG 5 cut(s) 263, 308, 335, 404, 1156
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 3 cut(s) 44, 163, 298
BsmBI CGTCTC 3 cut(s) 44, 163, 298
BsnI GGCC 2 cut(s) 262, 302
Bsp1286I GDGCHC 3 cut(s) 276, 347, 1122
Bsp143I GATC 2 cut(s) 1004, 1238
Bsp68I TCGCGA 1 cut(s) 490
BspACI CCGC 4 cut(s) 4, 137, 286, 750
BspANI GGCC 2 cut(s) 262, 302
BspCNI CTCAG 5 cut(s) 255, 1051, 1068, 1090, 1195
BspDI ATCGAT 1 cut(s) 230
BspFNI CGCG 3 cut(s) 9, 139, 490
BspLI GGNNCC 2 cut(s) 552, 604
BspTI CTTAAG 1 cut(s) 983
BsrDI GCAATG 2 cut(s) 1011, 1185
BsrFI RCCGGY 2 cut(s) 262, 334
BsrI ACTGG 3 cut(s) 329, 910, 948
BssAI RCCGGY 2 cut(s) 262, 334
BssECI CCNNGG 1 cut(s) 348
BssHII GCGCGC 1 cut(s) 7
BssMI GATC 2 cut(s) 1004, 1238
Bst2UI CCWGG 2 cut(s) 532, 1018
Bst4CI ACNGT 1 cut(s) 575
Bst6I CTCTTC 4 cut(s) 61, 73, 107, 917
BstAFI CTTAAG 1 cut(s) 983
BstC8I GCNNGC 2 cut(s) 9, 264
BstDEI CTNAG 5 cut(s) 242, 1038, 1055, 1077, 1203
BstF5I GGATG 6 cut(s) 391, 474, 706, 747, 1030, 1066
BstFNI CGCG 3 cut(s) 9, 139, 490
BstHHI GCGC 3 cut(s) 9, 11, 141
BstKTI GATC 2 cut(s) 1007, 1241
BstMAI GTCTC 3 cut(s) 44, 163, 298
BstMBI GATC 2 cut(s) 1004, 1238
BstMWI GCNNNNNNNGC 6 cut(s) 17, 246, 363, 463, 576, 1034
BstNI CCWGG 2 cut(s) 532, 1018
BstNSI RCATGY 1 cut(s) 671
BstSCI CCNGG 2 cut(s) 530, 1016
BstUI CGCG 3 cut(s) 9, 139, 490
BstV1I GCAGC 2 cut(s) 375, 784
BstXI CCANNNNNNTGG 2 cut(s) 257, 433
Bsu15I ATCGAT 1 cut(s) 230
Bsu36I CCTNAGG 1 cut(s) 1055
BsuRI GGCC 2 cut(s) 262, 302
BsuTUI ATCGAT 1 cut(s) 230
BtsCI GGATG 6 cut(s) 391, 474, 706, 747, 1030, 1066
BtuMI TCGCGA 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 9, 264
CfoI GCGC 3 cut(s) 9, 11, 141
Cfr10I RCCGGY 2 cut(s) 262, 334
Cfr13I GGNCC 3 cut(s) 260, 551, 603
ClaI ATCGAT 1 cut(s) 230
CseI GACGC 3 cut(s) 26, 26, 280
CsiI ACCWGGT 1 cut(s) 530
Csp6I GTAC 3 cut(s) 114, 646, 798
CviAII CATG 5 cut(s) 34, 583, 632, 668, 738
CviQI GTAC 3 cut(s) 114, 646, 798
DdeI CTNAG 5 cut(s) 242, 1038, 1055, 1077, 1203
DpnI GATC 2 cut(s) 1006, 1240
DpnII GATC 2 cut(s) 1004, 1238
DraI TTTAAA 1 cut(s) 514
DriI GACNNNNNGTC 1 cut(s) 36
Eam1104I CTCTTC 4 cut(s) 61, 73, 107, 917
Eam1105I GACNNNNNGTC 1 cut(s) 36
EarI CTCTTC 4 cut(s) 61, 73, 107, 917
EciI GGCGGA 1 cut(s) 765
Eco24I GRGCYC 1 cut(s) 276
Eco47I GGWCC 2 cut(s) 551, 603
Eco57I CTGAAG 1 cut(s) 1121
Eco81I CCTNAGG 1 cut(s) 1055
EcoO109I RGGNCCY 1 cut(s) 551
EcoRII CCWGG 2 cut(s) 530, 1016
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 276
Esp3I CGTCTC 3 cut(s) 44, 163, 298
FaeI CATG 5 cut(s) 37, 586, 635, 671, 741
FatI CATG 5 cut(s) 33, 582, 631, 667, 737
Fnu4HI GCNGC 4 cut(s) 5, 137, 364, 773
FokI GGATG 6 cut(s) 398, 461, 713, 754, 1037, 1073
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 4 cut(s) 5, 137, 364, 773
FspBI CTAG 4 cut(s) 128, 429, 555, 1089
GlaI GCGC 3 cut(s) 8, 10, 140
GluI GCNGC 4 cut(s) 5, 137, 364, 773
GsaI CCCAGC 2 cut(s) 136, 240
HaeIII GGCC 2 cut(s) 262, 302
HapII CCGG 5 cut(s) 263, 308, 335, 404, 1156
HgaI GACGC 3 cut(s) 26, 26, 280
HhaI GCGC 3 cut(s) 9, 11, 141
Hin1II CATG 5 cut(s) 37, 586, 635, 671, 741
Hin6I GCGC 3 cut(s) 7, 9, 139
HinP1I GCGC 3 cut(s) 7, 9, 139
HindIII AAGCTT 2 cut(s) 445, 986
HinfI GANTC 2 cut(s) 232, 979
HpaII CCGG 5 cut(s) 263, 308, 335, 404, 1156
HphI GGTGA 3 cut(s) 14, 638, 724
Hpy166II GTNNAC 2 cut(s) 629, 876
Hpy188I TCNGA 9 cut(s) 123, 245, 349, 410, 538, 1009, 1041, 1080, 1165
Hpy188III TCNNGA 8 cut(s) 28, 295, 489, 675, 833, 890, 1094, 1139
Hpy8I GTNNAC 2 cut(s) 629, 876
Hpy99I CGWCG 3 cut(s) 301, 362, 490
HpyAV CCTTC 6 cut(s) 70, 90, 204, 740, 946, 1015
HpyCH4III ACNGT 1 cut(s) 575
HpyCH4IV ACGT 2 cut(s) 485, 843
HpyCH4V TGCA 4 cut(s) 671, 772, 869, 1190
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 246, 363, 463, 576, 1034
HpyF3I CTNAG 5 cut(s) 242, 1038, 1055, 1077, 1203
HpySE526I ACGT 2 cut(s) 485, 843
Hsp92II CATG 5 cut(s) 37, 586, 635, 671, 741
HspAI GCGC 3 cut(s) 7, 9, 139
KroI GCCGGC 1 cut(s) 262
KroNI GCCGGC 1 cut(s) 264
Kzo9I GATC 2 cut(s) 1004, 1238
LmnI GCTCC 6 cut(s) 146, 350, 454, 523, 567, 1117
Lsp1109I GCAGC 2 cut(s) 375, 784
LweI GCATC 2 cut(s) 680, 1015
MabI ACCWGGT 1 cut(s) 530
MaeI CTAG 4 cut(s) 128, 429, 555, 1089
MaeII ACGT 2 cut(s) 485, 843
MalI GATC 2 cut(s) 1006, 1240
MboI GATC 2 cut(s) 1004, 1238
MhlI GDGCHC 3 cut(s) 276, 347, 1122
Mph1103I ATGCAT 1 cut(s) 673
MroNI GCCGGC 1 cut(s) 262
MroXI GAANNNNTTC 1 cut(s) 1146
MseI TTAA 8 cut(s) 513, 560, 618, 885, 915, 984, 1073, 1101
MslI CAYNNNNRTG 2 cut(s) 431, 740
MspCI CTTAAG 1 cut(s) 983
MspI CCGG 5 cut(s) 263, 308, 335, 404, 1156
MspR9I CCNGG 2 cut(s) 532, 1018
MvaI CCWGG 2 cut(s) 532, 1018
MvnI CGCG 3 cut(s) 9, 139, 490
MwoI GCNNNNNNNGC 6 cut(s) 17, 246, 363, 463, 576, 1034
NaeI GCCGGC 1 cut(s) 264
NdeII GATC 2 cut(s) 1004, 1238
NgoMIV GCCGGC 1 cut(s) 262
NlaIII CATG 5 cut(s) 37, 586, 635, 671, 741
NlaIV GGNNCC 2 cut(s) 552, 604
NruI TCGCGA 1 cut(s) 490
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 1 cut(s) 671
OliI CACNNNNGTG 1 cut(s) 431
PalAI GGCGCGCC 1 cut(s) 7
PauI GCGCGC 1 cut(s) 7
PdiI GCCGGC 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 1146
PfeI GAWTC 2 cut(s) 232, 979
PkrI GCNGC 4 cut(s) 6, 138, 365, 774
PpuMI RGGWCCY 1 cut(s) 551
PshBI ATTAAT 1 cut(s) 885
Psp5II RGGWCCY 1 cut(s) 551
Psp6I CCWGG 2 cut(s) 530, 1016
PspFI CCCAGC 2 cut(s) 132, 236
PspGI CCWGG 2 cut(s) 530, 1016
PspN4I GGNNCC 2 cut(s) 552, 604
PspPI GGNCC 3 cut(s) 260, 551, 603
PspPPI RGGWCCY 1 cut(s) 551
PteI GCGCGC 1 cut(s) 7
RruI TCGCGA 1 cut(s) 490
RsaI GTAC 3 cut(s) 115, 647, 799
RsaNI GTAC 3 cut(s) 114, 646, 798
RseI CAYNNNNRTG 2 cut(s) 431, 740
SaqAI TTAA 8 cut(s) 513, 560, 618, 885, 915, 984, 1073, 1101
SatI GCNGC 4 cut(s) 5, 137, 364, 773
Sau3AI GATC 2 cut(s) 1004, 1238
Sau96I GGNCC 3 cut(s) 260, 551, 603
ScrFI CCNGG 2 cut(s) 532, 1018
SduI GDGCHC 3 cut(s) 276, 347, 1122
SexAI ACCWGGT 1 cut(s) 530
SfaNI GCATC 2 cut(s) 680, 1015
SgsI GGCGCGCC 1 cut(s) 7
SinI GGWCC 2 cut(s) 551, 603
SmiMI CAYNNNNRTG 2 cut(s) 431, 740
SmlI CTYRAG 2 cut(s) 26, 983
SmoI CTYRAG 2 cut(s) 26, 983
SpeI ACTAGT 1 cut(s) 428
SsiI CCGC 4 cut(s) 4, 137, 286, 750
SspI AATATT 2 cut(s) 400, 1126
SspMI CTAG 4 cut(s) 128, 429, 555, 1089
StyD4I CCNGG 2 cut(s) 530, 1016
TaaI ACNGT 1 cut(s) 575
TaiI ACGT 2 cut(s) 488, 846
TaqI TCGA 2 cut(s) 230, 296
TauI GCSGC 2 cut(s) 7, 139
TfiI GAWTC 2 cut(s) 232, 979
Tru1I TTAA 8 cut(s) 513, 560, 618, 885, 915, 984, 1073, 1101
Tru9I TTAA 8 cut(s) 513, 560, 618, 885, 915, 984, 1073, 1101
TseI GCWGC 2 cut(s) 363, 772
TspDTI ATGAA 3 cut(s) 463, 935, 1077
TspGWI ACGGA 5 cut(s) 97, 101, 133, 145, 836
Vha464I CTTAAG 1 cut(s) 983
VpaK11BI GGWCC 2 cut(s) 551, 603
VspI ATTAAT 1 cut(s) 885
XapI RAATTY 6 cut(s) 469, 515, 911, 958, 1064, 1143
XceI RCATGY 1 cut(s) 671
XmnI GAANNNNTTC 1 cut(s) 1146
XspI CTAG 4 cut(s) 128, 429, 555, 1089
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.