MD07G1180700.v1.1

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014410
Physical Location & Seq
Reverse (-)
25990960 .. 25993554
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1180700.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGCGGCGCGCCATCGACGCCCACCTCAAGGCGGCGCGTCTCTTTCCCTCCTTCTCCTCCTTCTCTTCTTCCTCCTCCCCATCCACTCCTCCCGTCACCGTCCGTACAATCATAACCCTACTCCATCCCCACTCTCCTTCCATTTCCAAAACCTTAAACCGCCATTCGATCACAACTTCTCCGTGCTCTTCGATTCCGACTCTCAGAATCCACGGCATCGCCTCCAATTTCGCCCTCGCCGTCGTGCGCTGCATTTCGTCGGTCCCACATGCCGATACGTTGGAGTGGAACGAGCCGGTGTCGTGCTCGGAGGTCGGCGATGGCTACAAAGCCACCGTGGACGAAGACTCCAAGCCCTCGATTCCTGTTCGAGCATACTTCTTCTCCACCAGTGTGGATTTAAGAAGCTTGGTGGAGCAGAACAGGCTGAATTTCATCCCACCTACGTCGCGAATGACTAATTACGTTGTTTTTAAGTTCGGGGAGCTTTCCGATTCTAATGACGGTTTGGGTCTTAGTTTAAGTGGGAGTGACTGTTGTTACATGGTAGTCTTTCAGTATGGCTCCATTGTCTTGTTCAATGTCCGTGAACAAGATATCGATGGGTATTTGAAAATCGTGGAAAAACACGCATCGGGATCACTACCTGAAATGAGAAAGGATGAGTATGAGGTGAGAGAAAAGCCCACTTTGCACACATGGATGGAAGGTGGATTAGATTACATAATGTTGCAGTACTTGAATGTTGATGGAATCCGTACCATTGGTAGTGTTCTAGGTCAAAGTATTGCACTTGATTACTATGTGCGCCAGGTTGATGGAATGGTAGCAGAATTTACGGACATTAATCGTGAAATGGAAACTACTGGGAAGTTTAAAATGAAGAGGAAGAAACTGTTTCAGTTAGTGGGAAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTCGGACTTTTTGAGAGATCGGATATTGCCTGGAAGGATGCCAAATATGCTCAGATATGGGAATATCTCAGGGATGAATTTGAGTTAACTCAGAGATTTGCAAGTCTTGATTTTAAGTTGAAGTTTGTGGAGCACAATATTCGTTTTCTTCAGGAAATTCTCCAAAACAGGAAGTCAGATTTCTTGGAATGGCTCATCATTGCGTTAATAGGTGCTGAAATCCTTCTCTCCGTTTACGACATTGCCCACAGGTCGGCACTTGCTTCGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

407

Amino Acids

45.88

Weight (kDa)

6.38

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 182 - 352 1.6e-34 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13610
fragaria_vesca FvH4_7g20360
malus_domestica MD01G1113300.v1.1 MD07G1180700.v1.1
prunus_persica Prupe.2G220000_v2.0.a1
pyrus_communis pycom01g14160 pycom07g17400
rosa_chinensis RchiOBHm_Chr1g0364321
rosa_laevigata RLG00000027495
rosa_multiflora Rmu_sc0007470.1_g000002
rosa_roxburghii Rroxscaffold_4G00292430
rosa_rugosa Rorug01G0315400
rosa_samantha Rh1AG323100 Rh1BG285700 Rh1CG302400 Rh1DG317200
rosa_wichuraiana Rw1G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 9, 37, 451
AciI CCGC 3 cut(s) 4, 32, 160
AclWI GGATC 1 cut(s) 646
AcsI RAATTY 5 cut(s) 430, 833, 919, 1025, 1104
AcuI CTGAAG 1 cut(s) 1082
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 3 cut(s) 106, 737, 760
AfiI CCNNNNNNNGG 3 cut(s) 28, 31, 1201
AflII CTTAAG 1 cut(s) 944
AgsI TTSAA 4 cut(s) 580, 613, 742, 1069
AjnI CCWGG 2 cut(s) 810, 977
AleI CACNNNNGTG 1 cut(s) 392
AluBI AGCT 3 cut(s) 408, 487, 949
AluI AGCT 3 cut(s) 408, 487, 949
Alw21I GWGCWC 3 cut(s) 188, 308, 1083
Alw26I GTCTC 1 cut(s) 44
AlwI GGATC 1 cut(s) 646
ApeKI GCWGC 1 cut(s) 249
ApoI RAATTY 5 cut(s) 430, 833, 919, 1025, 1104
AscI GGCGCGCC 1 cut(s) 7
AseI ATTAAT 1 cut(s) 846
Asp700I GAANNNNTTC 1 cut(s) 1170
AspLEI GCGC 5 cut(s) 9, 11, 37, 249, 810
AspS9I GGNCC 1 cut(s) 262
AsuHPI GGTGA 2 cut(s) 88, 685
AvaII GGWCC 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 3 cut(s) 188, 308, 1083
BbvI GCAGC 1 cut(s) 236
BccI CCATC 8 cut(s) 20, 88, 132, 314, 596, 697, 743, 812
BceAI ACGGC 2 cut(s) 224, 229
BcgI CGANNNNNNTGC 3 cut(s) 1070, 1104, 1194
BciT130I CCWGG 2 cut(s) 812, 979
BcoDI GTCTC 1 cut(s) 44
BfaI CTAG 1 cut(s) 776
BfrI CTTAAG 1 cut(s) 944
BisI GCNGC 3 cut(s) 5, 33, 250
BlsI GCNGC 3 cut(s) 6, 34, 251
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 2 cut(s) 812, 979
Bme18I GGWCC 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 2 cut(s) 264, 565
BmrFI CCNGG 2 cut(s) 812, 979
BmrI ACTGGG 1 cut(s) 876
BmsI GCATC 3 cut(s) 225, 641, 976
BmuI ACTGGG 1 cut(s) 876
BpiI GAAGAC 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 600
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 211, 336
BsaXI ACNNNNNCTCC 8 cut(s) 163, 193, 332, 362, 368, 398, 520, 550
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 31, 1201
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 2 cut(s) 390, 871
Bse3DI GCAATG 2 cut(s) 1146, 1188
BseBI CCWGG 2 cut(s) 812, 979
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 2 cut(s) 211, 336
BseGI GGATG 7 cut(s) 80, 124, 435, 667, 708, 991, 1027
BseLI CCNNNNNNNGG 3 cut(s) 28, 31, 1201
BseMI GCAATG 2 cut(s) 1146, 1188
BseMII CTCAG 4 cut(s) 217, 1013, 1030, 1052
BseNI ACTGG 2 cut(s) 390, 871
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 3 cut(s) 46, 64, 78
BseXI GCAGC 1 cut(s) 236
Bsh1236I CGCG 3 cut(s) 9, 37, 451
BshVI ATCGAT 1 cut(s) 600
BsiHKAI GWGCWC 3 cut(s) 188, 308, 1083
BsiSI CCGG 1 cut(s) 296
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 28, 31, 1201
BsmAI GTCTC 1 cut(s) 44
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 248
Bsp1286I GDGCHC 3 cut(s) 188, 308, 1083
Bsp143I GATC 3 cut(s) 168, 638, 965
Bsp68I TCGCGA 1 cut(s) 451
BspACI CCGC 3 cut(s) 4, 32, 160
BspCNI CTCAG 4 cut(s) 216, 1012, 1029, 1051
BspDI ATCGAT 1 cut(s) 600
BspFNI CGCG 3 cut(s) 9, 37, 451
BspLI GGNNCC 2 cut(s) 264, 565
BspPI GGATC 1 cut(s) 646
BspQI GCTCTTC 1 cut(s) 193
BspTI CTTAAG 1 cut(s) 944
BsrDI GCAATG 2 cut(s) 1146, 1188
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 2 cut(s) 390, 871
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 2 cut(s) 211, 336
BssHII GCGCGC 1 cut(s) 7
BssMI GATC 3 cut(s) 168, 638, 965
BssNI GRCGYC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 812, 979
Bst4CI ACNGT 5 cut(s) 100, 337, 506, 536, 897
Bst6I CTCTTC 3 cut(s) 70, 193, 878
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 944
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 5 cut(s) 203, 515, 999, 1016, 1038
BstDSI CCRYGG 2 cut(s) 211, 336
BstF5I GGATG 7 cut(s) 80, 124, 435, 667, 708, 991, 1027
BstFNI CGCG 3 cut(s) 9, 37, 451
BstHHI GCGC 5 cut(s) 9, 11, 37, 249, 810
BstKTI GATC 3 cut(s) 171, 641, 968
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 3 cut(s) 168, 638, 965
BstMWI GCNNNNNNNGC 4 cut(s) 17, 424, 691, 995
BstNI CCWGG 2 cut(s) 812, 979
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 810, 977
BstUI CGCG 3 cut(s) 9, 37, 451
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 1 cut(s) 351
BstXI CCANNNNNNTGG 2 cut(s) 394, 818
Bsu15I ATCGAT 1 cut(s) 600
BsuTUI ATCGAT 1 cut(s) 600
BtgI CCRYGG 2 cut(s) 211, 336
BtgZI GCGATG 2 cut(s) 202, 333
BtsCI GGATG 7 cut(s) 80, 124, 435, 667, 708, 991, 1027
BtsIMutI CAGTG 1 cut(s) 397
BtuMI TCGCGA 1 cut(s) 451
Cac8I GCNNGC 1 cut(s) 9
CfoI GCGC 5 cut(s) 9, 11, 37, 249, 810
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 262
ClaI ATCGAT 1 cut(s) 600
CseI GACGC 2 cut(s) 26, 26
Csp6I GTAC 3 cut(s) 105, 736, 759
CviAII CATG 3 cut(s) 269, 544, 699
CviQI GTAC 3 cut(s) 105, 736, 759
DdeI CTNAG 5 cut(s) 203, 515, 999, 1016, 1038
DpnI GATC 3 cut(s) 170, 640, 967
DpnII GATC 3 cut(s) 168, 638, 965
DraI TTTAAA 1 cut(s) 877
Eam1104I CTCTTC 3 cut(s) 70, 193, 878
EarI CTCTTC 3 cut(s) 70, 193, 878
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 1082
EcoRII CCWGG 2 cut(s) 810, 977
EcoRV GATATC 1 cut(s) 598
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 3 cut(s) 272, 547, 702
FaqI GGGAC 1 cut(s) 248
FatI CATG 3 cut(s) 268, 543, 698
Fnu4HI GCNGC 3 cut(s) 5, 33, 250
FokI GGATG 7 cut(s) 67, 111, 422, 674, 715, 998, 1034
Fsp4HI GCNGC 3 cut(s) 5, 33, 250
FspBI CTAG 1 cut(s) 776
GlaI GCGC 5 cut(s) 8, 10, 36, 248, 809
GluI GCNGC 3 cut(s) 5, 33, 250
HapII CCGG 1 cut(s) 296
HgaI GACGC 2 cut(s) 26, 26
HhaI GCGC 5 cut(s) 9, 11, 37, 249, 810
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 3 cut(s) 272, 547, 702
Hin6I GCGC 5 cut(s) 7, 9, 35, 247, 808
HinP1I GCGC 5 cut(s) 7, 9, 35, 247, 808
HincII GTYRAC 1 cut(s) 1035
HindII GTYRAC 1 cut(s) 1035
HindIII AAGCTT 1 cut(s) 406
HinfI GANTC 8 cut(s) 193, 199, 207, 347, 361, 494, 753, 940
HpaI GTTAAC 1 cut(s) 1035
HpaII CCGG 1 cut(s) 296
HphI GGTGA 2 cut(s) 88, 685
Hpy166II GTNNAC 4 cut(s) 340, 590, 1035, 1183
Hpy188I TCNGA 9 cut(s) 198, 206, 310, 493, 953, 970, 1002, 1041, 1126
Hpy188III TCNNGA 5 cut(s) 450, 636, 851, 1055, 1100
Hpy8I GTNNAC 4 cut(s) 340, 590, 1035, 1183
Hpy99I CGWCG 4 cut(s) 20, 245, 262, 451
HpyAV CCTTC 6 cut(s) 61, 70, 147, 701, 976, 1181
HpyCH4III ACNGT 5 cut(s) 100, 337, 506, 536, 897
HpyCH4IV ACGT 3 cut(s) 278, 446, 465
HpyCH4V TGCA 5 cut(s) 252, 694, 733, 791, 1049
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 424, 691, 995
HpyF3I CTNAG 5 cut(s) 203, 515, 999, 1016, 1038
HpySE526I ACGT 3 cut(s) 278, 446, 465
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 3 cut(s) 272, 547, 702
HspAI GCGC 5 cut(s) 7, 9, 35, 247, 808
KspAI GTTAAC 1 cut(s) 1035
Kzo9I GATC 3 cut(s) 168, 638, 965
LguI GCTCTTC 1 cut(s) 193
LmnI GCTCC 4 cut(s) 415, 484, 569, 1078
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 3 cut(s) 225, 641, 976
MaeI CTAG 1 cut(s) 776
MaeII ACGT 3 cut(s) 278, 446, 465
MaeIII GTNAC 3 cut(s) 94, 530, 539
MalI GATC 3 cut(s) 170, 640, 967
MboI GATC 3 cut(s) 168, 638, 965
MboII GAAGA 8 cut(s) 57, 60, 180, 356, 373, 895, 901, 1088
MhlI GDGCHC 3 cut(s) 188, 308, 1083
MluCI AATT 7 cut(s) 226, 430, 460, 833, 919, 1025, 1104
MlyI GAGTC 2 cut(s) 193, 341
MmeI TCCRAC 2 cut(s) 221, 261
MroXI GAANNNNTTC 1 cut(s) 1170
MslI CAYNNNNRTG 2 cut(s) 392, 701
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 2 cut(s) 812, 979
MvaI CCWGG 2 cut(s) 812, 979
MvnI CGCG 3 cut(s) 9, 37, 451
MwoI GCNNNNNNNGC 4 cut(s) 17, 424, 691, 995
NdeII GATC 3 cut(s) 168, 638, 965
NlaIII CATG 3 cut(s) 272, 547, 702
NlaIV GGNNCC 2 cut(s) 264, 565
NmuCI GTSAC 2 cut(s) 94, 530
NruI TCGCGA 1 cut(s) 451
NspI RCATGY 1 cut(s) 272
OliI CACNNNNGTG 1 cut(s) 392
PalAI GGCGCGCC 1 cut(s) 7
PauI GCGCGC 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 193
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 1170
PfeI GAWTC 6 cut(s) 193, 207, 361, 494, 753, 940
PkrI GCNGC 3 cut(s) 6, 34, 251
PleI GAGTC 2 cut(s) 193, 341
PpsI GAGTC 2 cut(s) 193, 341
PshBI ATTAAT 1 cut(s) 846
Psp6I CCWGG 2 cut(s) 810, 977
PspGI CCWGG 2 cut(s) 810, 977
PspN4I GGNNCC 2 cut(s) 264, 565
PspPI GGNCC 1 cut(s) 262
PteI GCGCGC 1 cut(s) 7
RruI TCGCGA 1 cut(s) 451
RsaI GTAC 3 cut(s) 106, 737, 760
RsaNI GTAC 3 cut(s) 105, 736, 759
RseI CAYNNNNRTG 2 cut(s) 392, 701
SapI GCTCTTC 1 cut(s) 193
SatI GCNGC 3 cut(s) 5, 33, 250
Sau3AI GATC 3 cut(s) 168, 638, 965
Sau96I GGNCC 1 cut(s) 262
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 2 cut(s) 193, 341
ScrFI CCNGG 2 cut(s) 812, 979
SduI GDGCHC 3 cut(s) 188, 308, 1083
SfaNI GCATC 3 cut(s) 225, 641, 976
SgsI GGCGCGCC 1 cut(s) 7
SinI GGWCC 1 cut(s) 262
SmiMI CAYNNNNRTG 2 cut(s) 392, 701
SmlI CTYRAG 2 cut(s) 26, 944
SmoI CTYRAG 2 cut(s) 26, 944
Sse9I AATT 7 cut(s) 226, 430, 460, 833, 919, 1025, 1104
SsiI CCGC 3 cut(s) 4, 32, 160
SspI AATATT 1 cut(s) 1087
SspMI CTAG 1 cut(s) 776
StyD4I CCNGG 2 cut(s) 810, 977
TaaI ACNGT 5 cut(s) 100, 337, 506, 536, 897
TaiI ACGT 3 cut(s) 281, 449, 468
TaqI TCGA 6 cut(s) 15, 167, 191, 359, 370, 600
TaqII GACCGA 1 cut(s) 250
TasI AATT 7 cut(s) 226, 430, 460, 833, 919, 1025, 1104
TatI WGTACW 1 cut(s) 735
TauI GCSGC 2 cut(s) 7, 35
TfiI GAWTC 6 cut(s) 193, 207, 361, 494, 753, 940
TscAI CASTG 1 cut(s) 397
TseFI GTSAC 2 cut(s) 94, 530
TseI GCWGC 1 cut(s) 249
Tsp45I GTSAC 2 cut(s) 94, 530
TspDTI ATGAA 3 cut(s) 424, 896, 1038
TspGWI ACGGA 6 cut(s) 92, 171, 575, 746, 854, 1168
TspRI CASTG 1 cut(s) 397
Vha464I CTTAAG 1 cut(s) 944
VpaK11BI GGWCC 1 cut(s) 262
VspI ATTAAT 1 cut(s) 846
XapI RAATTY 5 cut(s) 430, 833, 919, 1025, 1104
XceI RCATGY 1 cut(s) 272
XmnI GAANNNNTTC 1 cut(s) 1170
XspI CTAG 1 cut(s) 776
ZrmI AGTACT 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.