Rh1DG317200

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014410
Physical Location & Seq
Reverse (-)
54340180 .. 54343453
3274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG317200.1

Sequence Viewer

Length: 1257 bp
ATGCGGCGCGCCATAGACGCCCACCTCAAAACCATGCGTCTCTTTCCTCCCCTCTCTTCCTTCTCCTCTTCTCCTCCTCCTCCTCCTTCTTCTTCCACGCCTCTCTTCTCCGTCCGTACATTTCTGACCCTAGCCCAGCCGCGCTCCGTTTCCAATTCCGTCTCCAAAACCCTAATTTTCCACTCAATCGCCACTCCTTCCTACACTTCTTCTTCTTCCTCCTCATCGATTCCCAGCCTCAGAAGCCACAGACTTGGGCCGGCTATTGGGCTCTTTGCCGTGCGGTGCGTTTCGACGGCCTCGCCGGAGAGCAGTTTGGACTGGAACGAGCCGGTGTCGTGCTCCGAGGTCGGCGACGGCAGCAAAGGAAGTGTTGATAAGGATGATAAGCCCAATATTCCGGTCAGAGCTTACTTCTTTTCCACTAGTGTGGATTTGAGAAGCTTGGTGGAGCAGAACAGGCTGAATTTCATCCCTCCTACGTCGCGAATGACCAATTATGTTGTGTTTAAATTCGGGGAGCTTACCAGGTCTGACAATGCTTTGGGTCCTAGTTTAAGTGGGAGCGACTGTTGCTACATGGTTGTTTTTCAATATGGGTCCATTGTCTTGTTTAATGTTCGTGAACATGAGATTGATGGGTACTTGAAAATTGTGGAAAAACATGCATCTGGATTGCTACCTGAAATGAGAAAGGATGAGTATGAGGTGAGAGAGAAACCAGATTTGGACACATGGATGGAAGGCGGATTGGATTACATAATGTTGCAGCACTTGAATACTGATGGTATTCGTACCATTGGTAGTGTTCTTGGTCAAAGTATTGCTCTGGATTACTACGTCCGCCAGGTTGATGGAATGGTTGCAGAGTTTACTGACATTAATCGTGAAATGGAAACTACTGGAAAATTTAAGATGAAGAGGAAGAAACTATTTCAGCTGGTGGGGAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTTGGACTTTTTGAGAGATCAGACATTGCCTGGAAGGATGCCAAATATGCTCAGATATGGGAATACCTCAGGGATGAATTTGAATTAACTCAGAGATTTGCTAGTCTTGACTTTAAGTTGAAGTTTGTGGAGCACAATATTCGCTTTCTTCAGGAAATTCTTCAAAACCGGAAATCAGATTTTTTGGAGTGGCTCATCATTGCACTAATAGGTGCTGAGATTATGCTCTCTCTTTATGACATTCTCCACAGATCAGCACTTGCTTCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

418

Amino Acids

47.16

Weight (kDa)

6.73

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 194 - 364 9.8e-35 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13610
fragaria_vesca FvH4_7g20360
malus_domestica MD01G1113300.v1.1 MD07G1180700.v1.1
prunus_persica Prupe.2G220000_v2.0.a1
pyrus_communis pycom01g14160 pycom07g17400
rosa_chinensis RchiOBHm_Chr1g0364321
rosa_laevigata RLG00000027495
rosa_multiflora Rmu_sc0007470.1_g000002
rosa_roxburghii Rroxscaffold_4G00292430
rosa_rugosa Rorug01G0315400
rosa_samantha Rh1AG323100 Rh1BG285700 Rh1CG302400 Rh1DG317200
rosa_wichuraiana Rw1G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 9, 142, 487
AciI CCGC 5 cut(s) 4, 140, 283, 747, 844
AcsI RAATTY 6 cut(s) 466, 512, 908, 955, 1061, 1140
AcuI CTGAAG 1 cut(s) 1118
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 3 cut(s) 118, 644, 796
AfiI CCNNNNNNNGG 1 cut(s) 266
AflII CTTAAG 1 cut(s) 980
AgsI TTSAA 6 cut(s) 593, 649, 778, 1067, 1105, 1148
AhlI ACTAGT 1 cut(s) 425
AjnI CCWGG 3 cut(s) 527, 846, 1013
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AleI CACNNNNGTG 1 cut(s) 428
AluBI AGCT 5 cut(s) 410, 444, 523, 940, 985
AluI AGCT 5 cut(s) 410, 444, 523, 940, 985
Alw21I GWGCWC 2 cut(s) 344, 1119
Alw26I GTCTC 2 cut(s) 44, 166
AoxI GGCC 2 cut(s) 257, 297
ApeKI GCWGC 2 cut(s) 360, 769
ApoI RAATTY 6 cut(s) 466, 512, 908, 955, 1061, 1140
AscI GGCGCGCC 1 cut(s) 7
AseI ATTAAT 1 cut(s) 882
Asp700I GAANNNNTTC 1 cut(s) 1143
AspLEI GCGC 3 cut(s) 9, 11, 144
AspS9I GGNCC 3 cut(s) 257, 548, 600
AsuHPI GGTGA 1 cut(s) 721
AvaII GGWCC 2 cut(s) 548, 600
AxyI CCTNAGG 1 cut(s) 1052
BanII GRGCYC 1 cut(s) 273
Bbv12I GWGCWC 2 cut(s) 344, 1119
BbvI GCAGC 2 cut(s) 372, 781
BccI CCATC 4 cut(s) 632, 733, 779, 848
BceAI ACGGC 3 cut(s) 263, 312, 373
BcgI CGANNNNNNTGC 2 cut(s) 1106, 1140
BciT130I CCWGG 3 cut(s) 529, 848, 1015
BcoDI GTCTC 2 cut(s) 44, 166
BcuI ACTAGT 1 cut(s) 425
BfaI CTAG 4 cut(s) 131, 426, 552, 1086
BfrI CTTAAG 1 cut(s) 980
BisI GCNGC 4 cut(s) 5, 140, 361, 770
BlsI GCNGC 4 cut(s) 6, 141, 362, 771
Bme1390I CCNGG 3 cut(s) 529, 848, 1015
Bme18I GGWCC 2 cut(s) 548, 600
BmgT120I GGNCC 3 cut(s) 257, 548, 600
BmiI GGNNCC 2 cut(s) 549, 601
BmrFI CCNGG 3 cut(s) 529, 848, 1015
BmsI GCATC 2 cut(s) 677, 1012
Bsa29I ATCGAT 1 cut(s) 227
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 345
BsaWI WCCGGW 2 cut(s) 400, 1152
BsaXI ACNNNNNCTCC 2 cut(s) 556, 586
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse118I RCCGGY 2 cut(s) 259, 331
Bse1I ACTGG 2 cut(s) 326, 907
Bse21I CCTNAGG 1 cut(s) 1052
Bse3DI GCAATG 2 cut(s) 1008, 1182
BseBI CCWGG 3 cut(s) 529, 848, 1015
BseCI ATCGAT 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 6 cut(s) 388, 471, 703, 744, 1027, 1063
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMI GCAATG 2 cut(s) 1008, 1182
BseMII CTCAG 5 cut(s) 253, 1049, 1066, 1088, 1191
BseNI ACTGG 2 cut(s) 326, 907
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 6 cut(s) 55, 63, 66, 69, 72, 211
BseXI GCAGC 2 cut(s) 372, 781
BseYI CCCAGC 2 cut(s) 135, 233
Bsh1236I CGCG 3 cut(s) 9, 142, 487
BshFI GGCC 2 cut(s) 259, 299
BshVI ATCGAT 1 cut(s) 227
BsiHKAI GWGCWC 2 cut(s) 344, 1119
BsiSI CCGG 5 cut(s) 260, 305, 332, 401, 1153
BslI CCNNNNNNNGG 1 cut(s) 266
BsmAI GTCTC 2 cut(s) 44, 166
BsmBI CGTCTC 2 cut(s) 44, 166
BsnI GGCC 2 cut(s) 259, 299
Bsp1286I GDGCHC 3 cut(s) 273, 344, 1119
Bsp143I GATC 2 cut(s) 1001, 1235
Bsp68I TCGCGA 1 cut(s) 487
BspACI CCGC 5 cut(s) 4, 140, 283, 747, 844
BspANI GGCC 2 cut(s) 259, 299
BspCNI CTCAG 5 cut(s) 252, 1048, 1065, 1087, 1192
BspDI ATCGAT 1 cut(s) 227
BspFNI CGCG 3 cut(s) 9, 142, 487
BspLI GGNNCC 2 cut(s) 549, 601
BspTI CTTAAG 1 cut(s) 980
BsrDI GCAATG 2 cut(s) 1008, 1182
BsrFI RCCGGY 2 cut(s) 259, 331
BsrI ACTGG 2 cut(s) 326, 907
BssAI RCCGGY 2 cut(s) 259, 331
BssECI CCNNGG 1 cut(s) 345
BssHII GCGCGC 1 cut(s) 7
BssMI GATC 2 cut(s) 1001, 1235
BssNI GRCGYC 1 cut(s) 18
Bst2UI CCWGG 3 cut(s) 529, 848, 1015
Bst4CI ACNGT 1 cut(s) 572
Bst6I CTCTTC 4 cut(s) 61, 73, 110, 914
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 980
BstC8I GCNNGC 2 cut(s) 9, 261
BstDEI CTNAG 5 cut(s) 239, 1035, 1052, 1074, 1200
BstF5I GGATG 6 cut(s) 388, 471, 703, 744, 1027, 1063
BstFNI CGCG 3 cut(s) 9, 142, 487
BstHHI GCGC 3 cut(s) 9, 11, 144
BstKTI GATC 2 cut(s) 1004, 1238
BstMAI GTCTC 2 cut(s) 44, 166
BstMBI GATC 2 cut(s) 1001, 1235
BstMWI GCNNNNNNNGC 6 cut(s) 17, 243, 360, 460, 573, 1031
BstNI CCWGG 3 cut(s) 529, 848, 1015
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 3 cut(s) 527, 846, 1013
BstUI CGCG 3 cut(s) 9, 142, 487
BstV1I GCAGC 2 cut(s) 372, 781
BstXI CCANNNNNNTGG 3 cut(s) 254, 430, 854
Bsu15I ATCGAT 1 cut(s) 227
Bsu36I CCTNAGG 1 cut(s) 1052
BsuRI GGCC 2 cut(s) 259, 299
BsuTUI ATCGAT 1 cut(s) 227
BtsCI GGATG 6 cut(s) 388, 471, 703, 744, 1027, 1063
BtuMI TCGCGA 1 cut(s) 487
Cac8I GCNNGC 2 cut(s) 9, 261
CfoI GCGC 3 cut(s) 9, 11, 144
Cfr10I RCCGGY 2 cut(s) 259, 331
Cfr13I GGNCC 3 cut(s) 257, 548, 600
ClaI ATCGAT 1 cut(s) 227
CseI GACGC 2 cut(s) 26, 26
CsiI ACCWGGT 1 cut(s) 527
Csp6I GTAC 3 cut(s) 117, 643, 795
CviAII CATG 5 cut(s) 34, 580, 629, 665, 735
CviQI GTAC 3 cut(s) 117, 643, 795
DdeI CTNAG 5 cut(s) 239, 1035, 1052, 1074, 1200
DpnI GATC 2 cut(s) 1003, 1237
DpnII GATC 2 cut(s) 1001, 1235
DraI TTTAAA 1 cut(s) 511
Eam1104I CTCTTC 4 cut(s) 61, 73, 110, 914
EarI CTCTTC 4 cut(s) 61, 73, 110, 914
EciI GGCGGA 2 cut(s) 762, 833
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 2 cut(s) 548, 600
Eco57I CTGAAG 1 cut(s) 1118
Eco81I CCTNAGG 1 cut(s) 1052
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 3 cut(s) 527, 846, 1013
EcoT22I ATGCAT 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 273
Esp3I CGTCTC 2 cut(s) 44, 166
FaeI CATG 5 cut(s) 37, 583, 632, 668, 738
FatI CATG 5 cut(s) 33, 579, 628, 664, 734
Fnu4HI GCNGC 4 cut(s) 5, 140, 361, 770
FokI GGATG 6 cut(s) 395, 458, 710, 751, 1034, 1070
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 4 cut(s) 5, 140, 361, 770
FspBI CTAG 4 cut(s) 131, 426, 552, 1086
GlaI GCGC 3 cut(s) 8, 10, 143
GluI GCNGC 4 cut(s) 5, 140, 361, 770
GsaI CCCAGC 2 cut(s) 139, 237
HaeIII GGCC 2 cut(s) 259, 299
HapII CCGG 5 cut(s) 260, 305, 332, 401, 1153
HgaI GACGC 2 cut(s) 26, 26
HhaI GCGC 3 cut(s) 9, 11, 144
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 5 cut(s) 37, 583, 632, 668, 738
Hin6I GCGC 3 cut(s) 7, 9, 142
HinP1I GCGC 3 cut(s) 7, 9, 142
HindIII AAGCTT 2 cut(s) 442, 983
HinfI GANTC 2 cut(s) 229, 976
HpaII CCGG 5 cut(s) 260, 305, 332, 401, 1153
HphI GGTGA 1 cut(s) 721
Hpy166II GTNNAC 2 cut(s) 626, 873
Hpy188I TCNGA 9 cut(s) 126, 242, 346, 407, 535, 1006, 1038, 1077, 1162
Hpy188III TCNNGA 7 cut(s) 486, 623, 672, 830, 887, 1091, 1136
Hpy8I GTNNAC 2 cut(s) 626, 873
Hpy99I CGWCG 3 cut(s) 298, 359, 487
HpyAV CCTTC 6 cut(s) 70, 96, 207, 737, 943, 1012
HpyCH4III ACNGT 1 cut(s) 572
HpyCH4IV ACGT 2 cut(s) 482, 840
HpyCH4V TGCA 4 cut(s) 668, 769, 866, 1187
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 243, 360, 460, 573, 1031
HpyF3I CTNAG 5 cut(s) 239, 1035, 1052, 1074, 1200
HpySE526I ACGT 2 cut(s) 482, 840
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 5 cut(s) 37, 583, 632, 668, 738
HspAI GCGC 3 cut(s) 7, 9, 142
KroI GCCGGC 1 cut(s) 259
KroNI GCCGGC 1 cut(s) 261
Kzo9I GATC 2 cut(s) 1001, 1235
LmnI GCTCC 6 cut(s) 149, 347, 451, 520, 564, 1114
Lsp1109I GCAGC 2 cut(s) 372, 781
LweI GCATC 2 cut(s) 677, 1012
MabI ACCWGGT 1 cut(s) 527
MaeI CTAG 4 cut(s) 131, 426, 552, 1086
MaeII ACGT 2 cut(s) 482, 840
MalI GATC 2 cut(s) 1003, 1237
MboI GATC 2 cut(s) 1001, 1235
MhlI GDGCHC 3 cut(s) 273, 344, 1119
Mph1103I ATGCAT 1 cut(s) 670
MroNI GCCGGC 1 cut(s) 259
MroXI GAANNNNTTC 1 cut(s) 1143
MseI TTAA 8 cut(s) 510, 557, 615, 882, 912, 981, 1070, 1098
MslI CAYNNNNRTG 2 cut(s) 428, 737
MspA1I CMGCKG 1 cut(s) 940
MspCI CTTAAG 1 cut(s) 980
MspI CCGG 5 cut(s) 260, 305, 332, 401, 1153
MspR9I CCNGG 3 cut(s) 529, 848, 1015
MvaI CCWGG 3 cut(s) 529, 848, 1015
MvnI CGCG 3 cut(s) 9, 142, 487
MwoI GCNNNNNNNGC 6 cut(s) 17, 243, 360, 460, 573, 1031
NaeI GCCGGC 1 cut(s) 261
NdeII GATC 2 cut(s) 1001, 1235
NgoMIV GCCGGC 1 cut(s) 259
NlaIII CATG 5 cut(s) 37, 583, 632, 668, 738
NlaIV GGNNCC 2 cut(s) 549, 601
NruI TCGCGA 1 cut(s) 487
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 1 cut(s) 668
OliI CACNNNNGTG 1 cut(s) 428
PalAI GGCGCGCC 1 cut(s) 7
PauI GCGCGC 1 cut(s) 7
PdiI GCCGGC 1 cut(s) 261
PdmI GAANNNNTTC 1 cut(s) 1143
PfeI GAWTC 2 cut(s) 229, 976
PkrI GCNGC 4 cut(s) 6, 141, 362, 771
PpuMI RGGWCCY 1 cut(s) 548
PshBI ATTAAT 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 3 cut(s) 527, 846, 1013
PspFI CCCAGC 2 cut(s) 135, 233
PspGI CCWGG 3 cut(s) 527, 846, 1013
PspN4I GGNNCC 2 cut(s) 549, 601
PspPI GGNCC 3 cut(s) 257, 548, 600
PspPPI RGGWCCY 1 cut(s) 548
PteI GCGCGC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 940
RruI TCGCGA 1 cut(s) 487
RsaI GTAC 3 cut(s) 118, 644, 796
RsaNI GTAC 3 cut(s) 117, 643, 795
RseI CAYNNNNRTG 2 cut(s) 428, 737
SaqAI TTAA 8 cut(s) 510, 557, 615, 882, 912, 981, 1070, 1098
SatI GCNGC 4 cut(s) 5, 140, 361, 770
Sau3AI GATC 2 cut(s) 1001, 1235
Sau96I GGNCC 3 cut(s) 257, 548, 600
ScrFI CCNGG 3 cut(s) 529, 848, 1015
SduI GDGCHC 3 cut(s) 273, 344, 1119
SexAI ACCWGGT 1 cut(s) 527
SfaNI GCATC 2 cut(s) 677, 1012
SgsI GGCGCGCC 1 cut(s) 7
SinI GGWCC 2 cut(s) 548, 600
SmiMI CAYNNNNRTG 2 cut(s) 428, 737
SmlI CTYRAG 1 cut(s) 980
SmoI CTYRAG 1 cut(s) 980
SpeI ACTAGT 1 cut(s) 425
SsiI CCGC 5 cut(s) 4, 140, 283, 747, 844
SspI AATATT 2 cut(s) 397, 1123
SspMI CTAG 4 cut(s) 131, 426, 552, 1086
StyD4I CCNGG 3 cut(s) 527, 846, 1013
TaaI ACNGT 1 cut(s) 572
TaiI ACGT 2 cut(s) 485, 843
TaqI TCGA 2 cut(s) 227, 293
TauI GCSGC 2 cut(s) 7, 142
TfiI GAWTC 2 cut(s) 229, 976
Tru1I TTAA 8 cut(s) 510, 557, 615, 882, 912, 981, 1070, 1098
Tru9I TTAA 8 cut(s) 510, 557, 615, 882, 912, 981, 1070, 1098
TseI GCWGC 2 cut(s) 360, 769
TspDTI ATGAA 3 cut(s) 460, 932, 1074
TspGWI ACGGA 4 cut(s) 100, 104, 136, 148
Vha464I CTTAAG 1 cut(s) 980
VpaK11BI GGWCC 2 cut(s) 548, 600
VspI ATTAAT 1 cut(s) 882
XapI RAATTY 6 cut(s) 466, 512, 908, 955, 1061, 1140
XceI RCATGY 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 1143
XspI CTAG 4 cut(s) 131, 426, 552, 1086
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.