Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011808

Physical Location & Seq

Genomic Coordinates Forward (+)

25188431 .. 25190860

Gene Structure 2430 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1142200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGACTCAGTCCCAGGACGATCCCGGGTTCTATCTCCCCTCCCACTTCCTCACGGACGACGTCGTGCTCCACAACAACATGGACGACAGCAAGGGCAACAGCTTCCGTGGAAACTGTCTCGAACCCCGCGCCAGATTCCCCACCGAGTTCCCGTACGAGTCCGACTCGTCCGACTCCAACTGGGCCCTGAGTTCCCCCGTCGAGTCCGTGGTGGGCTCCTCCGAGACCGAGGGCGGCAGCAGCAGCGACGAGGAGGACTTCCTCGCCGGCCTGACTCGCCGCCTTGGCCAGTCCTCGCTGCAGCCGGCTCACCAGACCCAGAAGCTATCCGTCCCAGACGCCTTCCCCAAAGAGAAACCCGAGTGGGTCATGTCCGGGTCTCCCCAGTCGATTCTGAGCGGAATCGGGAGCTGGTCGAGCAACGGAAGCCCGACGGGCCCGTCTTCCCAGGTGCCGTCGCCACCGACGACGCCGTTTGGAGCTCAGGACGACACCTGGGAGCTGATATACGCGGCAGCCGGGCAGGTTGCGCGGTTGAAGATGACTAACAGCTTTGGTGGAGCAACTGAGTTCAGCGGCAACCACGGCCGCCGCGGACTTATGGGGCCATCTCGGATCCCCAGTCCTCCCCAACACTCTGCTCCCGGTCTCTGCCCCAACCAGAGCTTCCCTCAGTTTCACCATGTGAGGCAGGCGAACCAAGTAAATCATCTTCCCTGGTCAGCTCAGCCGCAGCAGCAACCTCAGCAACACCACCAGCAGATCCAAACTAGAGGAAGAAACACTGCAGGTTACGAGAGATGCGGGCATGGTGTTAATTTGCCCCAATCGGCATGGCCGCCGCTGCAAGTCCAACACCACCATCAAAATCAACAGCCACAGCACCACAATAACGCCGCCGTGCGTCCCGTTTTGCCCAACGGTTCCAACATCAAGAGGGAATGCGCTGGCACCGGCGTTTTCTTGCCTCGCAGATATACAAACCCTGCCCCTCCTGAACCGCGCAAGAAAGCAGGCTGTCCAACTGTTCTACTGCCGGCCAAGGTTGTTCACGCTCTGAACCTGAGCTTCGAGGACATGAACCGCCACGCTCCGCCGCGTTTCAGCGCCGGTTTGGCGCCAGACTACGAGGCACTGGCGGCACGAAGAAACGCCCTGCTGACTCAGCAAAGACTTGGATTAAGGGCAGCGGCCGAGGGGCCGGTAAATCATGAACTGCGCCTCCCCCAGGAGTGGACCTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

415

Amino Acids

45.3

Weight (kDa)

7.04

Isoelectric Point (pI)

64.76

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39870
fragaria_vesca	FvH4_7g23630 FvH4_7g23630
malus_domestica	MD01G1142200.v1.1 MD07G1207900.v1.1
prunus_persica	Prupe.2G243200_v2.0.a1 Prupe.4G251600_v2.0.a1
pyrus_communis	pycom01g16240 pycom07g19360
rosa_chinensis	RchiOBHm_Chr1g0368901
rosa_laevigata	RLG00000027178
rosa_multiflora	Rmu_sc0004250.1_g000045 Rmu_sc0030064.1_g000001
rosa_roxburghii	Rroxscaffold_4G00288220
rosa_rugosa	Rorug01G0345700
rosa_samantha	Rh1AG352700 Rh1BG316200 Rh1CG330900 Rh1DG347700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	439
AatII	GACGTC	1 cut(s)	63
Acc36I	ACCTGC	2 cut(s)	514, 779
AccB1I	GGYRCC	3 cut(s)	451, 950, 1117
AccBSI	CCGCTC	1 cut(s)	399
AccII	CGCG	6 cut(s)	129, 512, 532, 594, 1003, 1099
AclWI	GGATC	4 cut(s)	14, 610, 623, 757
AcoI	YGGCCR	5 cut(s)	286, 586, 836, 1038, 1191
AcyI	GRCGYC	4 cut(s)	60, 339, 470, 1118
AdeI	CACNNNGTG	1 cut(s)	685
AfaI	GTAC	1 cut(s)	155
AfiI	CCNNNNNNNGG	2 cut(s)	1228, 1233
AgsI	TTSAA	1 cut(s)	538
AjnI	CCWGG	5 cut(s)	12, 447, 494, 716, 1227
AjuI	GAANNNNNNNTTGG	2 cut(s)	650, 682
AloI	GAACNNNNNNTCC	2 cut(s)	1206, 1238
AluBI	AGCT	9 cut(s)	102, 325, 411, 482, 502, 552, 666, 725, 1068
AluI	AGCT	9 cut(s)	102, 325, 411, 482, 502, 552, 666, 725, 1068
Alw21I	GWGCWC	2 cut(s)	69, 484
Alw26I	GTCTC	4 cut(s)	122, 218, 384, 653
AlwI	GGATC	4 cut(s)	14, 610, 623, 757
Ama87I	CYCGRG	2 cut(s)	23, 359
ApaI	GGGCCC	2 cut(s)	187, 440
AspLEI	GCGC	7 cut(s)	131, 532, 947, 1005, 1109, 1120, 1221
AspS9I	GGNCC	7 cut(s)	183, 184, 436, 437, 605, 1199, 1236
AsuC2I	CCSGG	5 cut(s)	24, 25, 376, 520, 645
AsuHPI	GGTGA	2 cut(s)	302, 671
AvaI	CYCGRG	2 cut(s)	23, 359
AvaII	GGWCC	1 cut(s)	1236
BaeGI	GKGCMC	2 cut(s)	187, 440
BalI	TGGCCA	1 cut(s)	288
BamHI	GGATCC	1 cut(s)	615
BanI	GGYRCC	3 cut(s)	451, 950, 1117
BanII	GRGCYC	4 cut(s)	187, 218, 440, 484
BarI	GAAGNNNNNNTAC	2 cut(s)	696, 728
BbsI	GAAGAC	1 cut(s)	435
Bbv12I	GWGCWC	2 cut(s)	69, 484
BbvCI	CCTCAGC	1 cut(s)	744
BccI	CCATC	2 cut(s)	616, 870
BceAI	ACGGC	4 cut(s)	439, 457, 601, 884
BciT130I	CCWGG	5 cut(s)	14, 449, 496, 718, 1229
BcnI	CCSGG	5 cut(s)	24, 25, 376, 520, 645
BcoDI	GTCTC	4 cut(s)	122, 218, 384, 653
BfaI	CTAG	1 cut(s)	771
BfmI	CTRYAG	2 cut(s)	299, 786
BfoI	RGCGCY	2 cut(s)	1110, 1121
BfuAI	ACCTGC	2 cut(s)	514, 779
BglI	GCCNNNNNGGC	3 cut(s)	285, 435, 1115
BlpI	GCTNAGC	1 cut(s)	726
Bme18I	GGWCC	1 cut(s)	1236
BmeT110I	CYCGRG	2 cut(s)	23, 359
BmgT120I	GGNCC	7 cut(s)	183, 184, 436, 437, 605, 1199, 1236
BmrI	ACTGGG	3 cut(s)	190, 379, 615
BmsI	GCATC	1 cut(s)	791
BmuI	ACTGGG	3 cut(s)	190, 379, 615
BpiI	GAAGAC	1 cut(s)	435
BplI	GAGNNNNNCTC	4 cut(s)	149, 181, 655, 687
Bpu10I	CCTNAGC	3 cut(s)	483, 744, 1064
Bpu1102I	GCTNAGC	1 cut(s)	726
BpuMI	CCSGG	5 cut(s)	24, 25, 376, 520, 645
BsaHI	GRCGYC	4 cut(s)	60, 339, 470, 1118
BsaI	GGTCTC	3 cut(s)	218, 384, 653
BsaXI	ACNNNNNCTCC	2 cut(s)	1206, 1236
Bsc4I	CCNNNNNNNGG	2 cut(s)	1228, 1233
Bse118I	RCCGGY	6 cut(s)	266, 304, 953, 1036, 1109, 1201
Bse1I	ACTGG	5 cut(s)	185, 289, 385, 621, 1140
BseBI	CCWGG	5 cut(s)	14, 449, 496, 718, 1229
BseLI	CCNNNNNNNGG	2 cut(s)	1228, 1233
BseNI	ACTGG	5 cut(s)	185, 289, 385, 621, 1140
BseRI	GAGGAG	2 cut(s)	208, 266
BseSI	GKGCMC	2 cut(s)	187, 440
BseX3I	CGGCCG	2 cut(s)	586, 1191
Bsh1236I	CGCG	6 cut(s)	129, 512, 532, 594, 1003, 1099
Bsh1285I	CGRYCG	2 cut(s)	589, 1194
BshNI	GGYRCC	3 cut(s)	451, 950, 1117
BsiEI	CGRYCG	2 cut(s)	589, 1194
BsiHKAI	GWGCWC	2 cut(s)	69, 484
BsiHKCI	CYCGRG	2 cut(s)	23, 359
BsiWI	CGTACG	1 cut(s)	153
BslFI	GGGAC	2 cut(s)	317, 891
BslI	CCNNNNNNNGG	2 cut(s)	1228, 1233
BsmAI	GTCTC	4 cut(s)	122, 218, 384, 653
BsmFI	GGGAC	2 cut(s)	317, 891
BsmI	GAATGC	1 cut(s)	947
Bso31I	GGTCTC	3 cut(s)	218, 384, 653
BsoBI	CYCGRG	2 cut(s)	23, 359
Bsp120I	GGGCCC	2 cut(s)	183, 436
Bsp1286I	GDGCHC	5 cut(s)	69, 187, 218, 440, 484
Bsp143I	GATC	3 cut(s)	19, 615, 762
Bsp1720I	GCTNAGC	1 cut(s)	726
BspFNI	CGCG	6 cut(s)	129, 512, 532, 594, 1003, 1099
BspHI	TCATGA	1 cut(s)	1210
BspMAI	CTGCAG	2 cut(s)	303, 790
BspMI	ACCTGC	2 cut(s)	514, 779
BspPI	GGATC	4 cut(s)	14, 610, 623, 757
BspT107I	GGYRCC	3 cut(s)	451, 950, 1117
BspTNI	GGTCTC	3 cut(s)	218, 384, 653
BsrBI	CCGCTC	1 cut(s)	399
BsrFI	RCCGGY	6 cut(s)	266, 304, 953, 1036, 1109, 1201
BsrI	ACTGG	5 cut(s)	185, 289, 385, 621, 1140
BssAI	RCCGGY	6 cut(s)	266, 304, 953, 1036, 1109, 1201
BssMI	GATC	3 cut(s)	19, 615, 762
BssNI	GRCGYC	4 cut(s)	60, 339, 470, 1118
BssT1I	CCWWGG	2 cut(s)	283, 1041
Bst2UI	CCWGG	5 cut(s)	14, 449, 496, 718, 1229
Bst4CI	ACNGT	3 cut(s)	116, 923, 1027
BstACI	GRCGYC	4 cut(s)	60, 339, 470, 1118
BstC8I	GCNNGC	7 cut(s)	268, 306, 693, 806, 949, 1015, 1038
BstDSI	CCRYGG	4 cut(s)	106, 207, 583, 592
BstENI	CCTNNNNNAGG	1 cut(s)	1226
BstFNI	CGCG	6 cut(s)	129, 512, 532, 594, 1003, 1099
BstH2I	RGCGCY	2 cut(s)	1110, 1121
BstHHI	GCGC	7 cut(s)	131, 532, 947, 1005, 1109, 1120, 1221
BstKTI	GATC	3 cut(s)	22, 618, 765
BstMAI	GTCTC	4 cut(s)	122, 218, 384, 653
BstMBI	GATC	3 cut(s)	19, 615, 762
BstMCI	CGRYCG	2 cut(s)	589, 1194
BstNI	CCWGG	5 cut(s)	14, 449, 496, 718, 1229
BstSFI	CTRYAG	2 cut(s)	299, 786
BstSLI	GKGCMC	2 cut(s)	187, 440
BstUI	CGCG	6 cut(s)	129, 512, 532, 594, 1003, 1099
BstV2I	GAAGAC	1 cut(s)	435
BstX2I	RGATCY	2 cut(s)	615, 762
BstYI	RGATCY	2 cut(s)	615, 762
BstZI	CGGCCG	2 cut(s)	586, 1191
BtgI	CCRYGG	4 cut(s)	106, 207, 583, 592
BtsI	GCAGTG	1 cut(s)	783
BtsIMutI	CAGTG	2 cut(s)	783, 1133
BveI	ACCTGC	2 cut(s)	514, 779
Cac8I	GCNNGC	7 cut(s)	268, 306, 693, 806, 949, 1015, 1038
CciI	TCATGA	1 cut(s)	1210
CfoI	GCGC	7 cut(s)	131, 532, 947, 1005, 1109, 1120, 1221
Cfr10I	RCCGGY	6 cut(s)	266, 304, 953, 1036, 1109, 1201
Cfr13I	GGNCC	7 cut(s)	183, 184, 436, 437, 605, 1199, 1236
Cfr42I	CCGCGG	1 cut(s)	595
Cfr9I	CCCGGG	1 cut(s)	23
CseI	GACGC	3 cut(s)	347, 478, 893
Csp6I	GTAC	1 cut(s)	154
CviAII	CATG	7 cut(s)	79, 370, 683, 809, 834, 1078, 1211
CviQI	GTAC	1 cut(s)	154
DinI	GGCGCC	1 cut(s)	1119
DpnI	GATC	3 cut(s)	21, 617, 764
DpnII	GATC	3 cut(s)	19, 615, 762
DraIII	CACNNNGTG	1 cut(s)	685
DrdI	GACNNNNNNGTC	1 cut(s)	439
DseDI	GACNNNNNNGTC	1 cut(s)	439
EaeI	YGGCCR	5 cut(s)	286, 586, 836, 1038, 1191
EagI	CGGCCG	2 cut(s)	586, 1191
EciI	GGCGGA	1 cut(s)	1083
Ecl136II	GAGCTC	1 cut(s)	482
EclXI	CGGCCG	2 cut(s)	586, 1191
Eco130I	CCWWGG	2 cut(s)	283, 1041
Eco24I	GRGCYC	4 cut(s)	187, 218, 440, 484
Eco31I	GGTCTC	3 cut(s)	218, 384, 653
Eco47I	GGWCC	1 cut(s)	1236
Eco52I	CGGCCG	2 cut(s)	586, 1191
Eco53kI	GAGCTC	1 cut(s)	482
Eco88I	CYCGRG	2 cut(s)	23, 359
EcoICRI	GAGCTC	1 cut(s)	482
EcoNI	CCTNNNNNAGG	1 cut(s)	1226
EcoO109I	RGGNCCY	1 cut(s)	184
EcoRII	CCWGG	5 cut(s)	12, 447, 494, 716, 1227
EcoT14I	CCWWGG	2 cut(s)	283, 1041
EcoT38I	GRGCYC	4 cut(s)	187, 218, 440, 484
EgeI	GGCGCC	1 cut(s)	1119
EheI	GGCGCC	1 cut(s)	1119
ErhI	CCWWGG	2 cut(s)	283, 1041
FaeI	CATG	7 cut(s)	82, 373, 686, 812, 837, 1081, 1214
FaqI	GGGAC	2 cut(s)	317, 891
FatI	CATG	7 cut(s)	78, 369, 682, 808, 833, 1077, 1210
FauI	CCCGC	2 cut(s)	134, 797
FriOI	GRGCYC	4 cut(s)	187, 218, 440, 484
FspBI	CTAG	1 cut(s)	771
GlaI	GCGC	7 cut(s)	130, 531, 946, 1004, 1108, 1119, 1220
HaeII	RGCGCY	2 cut(s)	1110, 1121
HgaI	GACGC	3 cut(s)	347, 478, 893
HhaI	GCGC	7 cut(s)	131, 532, 947, 1005, 1109, 1120, 1221
Hin1I	GRCGYC	4 cut(s)	60, 339, 470, 1118
Hin1II	CATG	7 cut(s)	82, 373, 686, 812, 837, 1081, 1214
Hin6I	GCGC	7 cut(s)	129, 530, 945, 1003, 1107, 1118, 1219
HinP1I	GCGC	7 cut(s)	129, 530, 945, 1003, 1107, 1118, 1219
HphI	GGTGA	2 cut(s)	302, 671
Hpy166II	GTNNAC	2 cut(s)	1051, 1236
Hpy188I	TCNGA	6 cut(s)	163, 172, 223, 396, 615, 1059
Hpy188III	TCNNGA	6 cut(s)	119, 406, 485, 934, 995, 1211
Hpy8I	GTNNAC	2 cut(s)	1051, 1236
Hpy99I	CGWCG	8 cut(s)	62, 65, 203, 251, 436, 460, 469, 472
HpyAV	CCTTC	1 cut(s)	352
HpyCH4III	ACNGT	3 cut(s)	116, 923, 1027
HpyCH4IV	ACGT	1 cut(s)	60
HpyCH4V	TGCA	3 cut(s)	301, 788, 847
HpySE526I	ACGT	1 cut(s)	60
Hsp92I	GRCGYC	4 cut(s)	60, 339, 470, 1118
Hsp92II	CATG	7 cut(s)	82, 373, 686, 812, 837, 1081, 1214
HspAI	GCGC	7 cut(s)	129, 530, 945, 1003, 1107, 1118, 1219
KasI	GGCGCC	1 cut(s)	1117
KroI	GCCGGC	3 cut(s)	266, 304, 1036
KroNI	GCCGGC	3 cut(s)	268, 306, 1038
KspI	CCGCGG	1 cut(s)	595
Kzo9I	GATC	3 cut(s)	19, 615, 762
LmnI	GCTCC	8 cut(s)	72, 221, 408, 479, 499, 560, 646, 1096
LweI	GCATC	1 cut(s)	791
MaeI	CTAG	1 cut(s)	771
MaeII	ACGT	1 cut(s)	60
MaeIII	GTNAC	1 cut(s)	791
MalI	GATC	3 cut(s)	21, 617, 764
MbiI	CCGCTC	1 cut(s)	399
MboI	GATC	3 cut(s)	19, 615, 762
MboII	GAAGA	5 cut(s)	435, 550, 704, 789, 1158
MflI	RGATCY	2 cut(s)	615, 762
MhlI	GDGCHC	5 cut(s)	69, 187, 218, 440, 484
MlsI	TGGCCA	1 cut(s)	288
MluCI	AATT	1 cut(s)	817
MluNI	TGGCCA	1 cut(s)	288
Mly113I	GGCGCC	1 cut(s)	1118
MlyI	GAGTC	6 cut(s)	158, 167, 167, 212, 268, 1156
MmeI	TCCRAC	6 cut(s)	186, 195, 201, 877, 951, 1046
Mox20I	TGGCCA	1 cut(s)	288
MroNI	GCCGGC	3 cut(s)	266, 304, 1036
MscI	TGGCCA	1 cut(s)	288
MseI	TTAA	2 cut(s)	816, 1181
Msp20I	TGGCCA	1 cut(s)	288
MspA1I	CMGCKG	4 cut(s)	576, 594, 844, 1190
Mva1269I	GAATGC	1 cut(s)	947
MvaI	CCWGG	5 cut(s)	14, 449, 496, 718, 1229
MvnI	CGCG	6 cut(s)	129, 512, 532, 594, 1003, 1099
NaeI	GCCGGC	3 cut(s)	268, 306, 1038
NarI	GGCGCC	1 cut(s)	1118
NciI	CCSGG	5 cut(s)	24, 25, 376, 520, 645
NdeII	GATC	3 cut(s)	19, 615, 762
NgoMIV	GCCGGC	3 cut(s)	266, 304, 1036
NlaIII	CATG	7 cut(s)	82, 373, 686, 812, 837, 1081, 1214
NmeAIII	GCCGAG	1 cut(s)	1219
PagI	TCATGA	1 cut(s)	1210
PcsI	WCGNNNNNNNCGW	1 cut(s)	464
PctI	GAATGC	1 cut(s)	947
PdiI	GCCGGC	3 cut(s)	268, 306, 1038
PfeI	GAWTC	3 cut(s)	135, 391, 402
Pfl23II	CGTACG	1 cut(s)	153
PflFI	GACNNNGTC	2 cut(s)	7, 59
PleI	GAGTC	6 cut(s)	158, 166, 167, 211, 268, 1156
PluTI	GGCGCC	1 cut(s)	1121
PpsI	GAGTC	6 cut(s)	158, 166, 167, 211, 268, 1156
Psp124BI	GAGCTC	1 cut(s)	484
Psp6I	CCWGG	5 cut(s)	12, 447, 494, 716, 1227
PspGI	CCWGG	5 cut(s)	12, 447, 494, 716, 1227
PspLI	CGTACG	1 cut(s)	153
PspOMI	GGGCCC	2 cut(s)	183, 436
PspPI	GGNCC	7 cut(s)	183, 184, 436, 437, 605, 1199, 1236
PstI	CTGCAG	2 cut(s)	303, 790
PsuI	RGATCY	2 cut(s)	615, 762
PsyI	GACNNNGTC	2 cut(s)	7, 59
RsaI	GTAC	1 cut(s)	155
RsaNI	GTAC	1 cut(s)	154
SacI	GAGCTC	1 cut(s)	484
SacII	CCGCGG	1 cut(s)	595
SaqAI	TTAA	2 cut(s)	816, 1181
Sau3AI	GATC	3 cut(s)	19, 615, 762
Sau96I	GGNCC	7 cut(s)	183, 184, 436, 437, 605, 1199, 1236
SchI	GAGTC	6 cut(s)	158, 167, 167, 212, 268, 1156
SduI	GDGCHC	5 cut(s)	69, 187, 218, 440, 484
SfaNI	GCATC	1 cut(s)	791
SfcI	CTRYAG	2 cut(s)	299, 786
SfoI	GGCGCC	1 cut(s)	1119
Sfr303I	CCGCGG	1 cut(s)	595
SgrAI	CRCCGGYG	1 cut(s)	953
SgrBI	CCGCGG	1 cut(s)	595
SinI	GGWCC	1 cut(s)	1236
SmaI	CCCGGG	1 cut(s)	25
Sse9I	AATT	1 cut(s)	817
SspDI	GGCGCC	1 cut(s)	1117
SspMI	CTAG	1 cut(s)	771
SstI	GAGCTC	1 cut(s)	484
StyI	CCWWGG	2 cut(s)	283, 1041
TaaI	ACNGT	3 cut(s)	116, 923, 1027
TaiI	ACGT	1 cut(s)	63
TaqI	TCGA	5 cut(s)	120, 201, 389, 416, 1071
TaqII	GACCGA	1 cut(s)	242
TasI	AATT	1 cut(s)	817
TfiI	GAWTC	3 cut(s)	135, 391, 402
Tru1I	TTAA	2 cut(s)	816, 1181
Tru9I	TTAA	2 cut(s)	816, 1181
TscAI	CASTG	2 cut(s)	790, 1140
TspDTI	ATGAA	2 cut(s)	1094, 1227
TspGWI	ACGGA	5 cut(s)	68, 95, 196, 319, 438
TspMI	CCCGGG	1 cut(s)	23
TspRI	CASTG	2 cut(s)	790, 1140
Tth111I	GACNNNGTC	2 cut(s)	7, 59
VpaK11BI	GGWCC	1 cut(s)	1236
XagI	CCTNNNNNAGG	1 cut(s)	1226
XmaI	CCCGGG	1 cut(s)	23
XspI	CTAG	1 cut(s)	771
ZraI	GACGTC	1 cut(s)	61

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.