MD07G1207900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011808
Physical Location & Seq
Forward (+)
28764667 .. 28767044
2378 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1207900.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGAATCACTCCCAGGACGATCCCGAGTTCCATCTCCCCTCCCACTTCCTCACGGACGACGTCGTGCTCCACTGCAACATGGGCGACAGCAACAAAGACCACAGCTTCCGCCGGAACTGCGTCGAATCCCGCGTCAGCTTCCCCACCGAGTTCCCCTACGAGTTCGACTCGTCCGACTCCTACTCGGCCCTGAACTCCCCTGTCGAGTCCGTGGTGAGCTCCACCGAGACCGAGGGAGGCAGCAGCAGCAGCGACGAGGAGGACTTCCTCGCCGGGCTTACCCGCCGCCTCGCACAGTCCTCGCTGCAGCCTGCTCACCAGACCCAGAAACTATCCGTCCCCGCCGCCTTCCCCAAAGAGAACCCCGAGTGGGTCATGTCCGGGTCTCCCCAGTCGACTCTGAGCGGAATCGGGAGCTGGTCCAGCAACGGAAGCCCGACGGGCCCGTCTTCCCAGGTGCCTTCGCCACCGACGACGCCGTTTGGAGCTCAGAACGACATCTGGGAGCTGATATACGCGGCAGCCGGGCAGGTTGCGCGGTTAAAAATGACCAACAGCCTCGGCGGAGCAACCGAGTTCGGCGGCAACCACGGCCGCGGTGGACTTCTGGGCCCACCTCGGATCCCCAGTCCTCCCCGACACTCAGGCCCCGGGCTCTGCTCGAACCAAAGCTTCACTCAGTTTCACCATGTGAGGCAGGACAAGCAAGTAAATCATCTTCCATGGTCAGCTCAGCCACATCAACCAGCTCAGCTCCACCCCCAGCACAACCACCAGCAGCAGATCCAAAACAGAGGAAGAAACATTGTTGGGTACGAGAGATGCGAGCATGGTGTTAATTTTCCCCAATCGACATGGCCGCCGCTTCAAGTCCAACACAACCACCACCACCACCACCACCATCACCAACACCCCCACCACCAACAAAGTCAGCAACCTCAGCGCCACACTAACGCCGCCGTGCGTCCCGTTTTGCCCAACGGTTCTAATATCAAGAGGGAATGTGCTGGCACCGGCGTTTTCTTGCCTCGCAGATACACCAACCCTTCCCCTCCTGAACCTCGCAAGAAAGCAGGTTGTTCAACTGTTCTAATGCCGGCCAAGGTTGTTCAGGCTCTGAACCTGAGCTTCGAAGACATGAACCGCCACGCTCCGCCGCGTTTCACCACCGGTTTGGCTCCAGACCATGGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

399

Amino Acids

43.87

Weight (kDa)

7.03

Isoelectric Point (pI)

64.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 445
AatII GACGTC 1 cut(s) 63
Acc36I ACCTGC 2 cut(s) 520, 1064
AccB1I GGYRCC 2 cut(s) 457, 1010
AccB7I CCANNNNNTGG 1 cut(s) 1187
AccBSI CCGCTC 1 cut(s) 405
AccI GTMKAC 1 cut(s) 395
AccII CGCG 5 cut(s) 132, 518, 538, 597, 1159
AclWI GGATC 4 cut(s) 14, 616, 629, 778
AcoI YGGCCR 3 cut(s) 592, 857, 1098
AcyI GRCGYC 2 cut(s) 60, 476
AdeI CACNNNGTG 1 cut(s) 691
AfaI GTAC 1 cut(s) 815
AfiI CCNNNNNNNGG 2 cut(s) 370, 1187
AgeI ACCGGT 1 cut(s) 1169
AgsI TTSAA 2 cut(s) 869, 1083
AjnI CCWGG 2 cut(s) 12, 453
Alw21I GWGCWC 3 cut(s) 69, 221, 490
Alw26I GTCTC 2 cut(s) 221, 390
AlwI GGATC 4 cut(s) 14, 616, 629, 778
AlwNI CAGNNNCTG 1 cut(s) 1117
Ama87I CYCGRG 3 cut(s) 23, 365, 650
AoxI GGCC 7 cut(s) 186, 442, 592, 610, 646, 857, 1098
ApaI GGGCCC 2 cut(s) 446, 614
ApeKI GCWGC 8 cut(s) 240, 243, 246, 249, 304, 307, 521, 778
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
AsiGI ACCGGT 1 cut(s) 1169
AspLEI GCGC 2 cut(s) 538, 945
AspS9I GGNCC 7 cut(s) 187, 420, 442, 443, 610, 611, 647
AsuC2I CCSGG 5 cut(s) 274, 382, 526, 651, 652
AsuHPI GGTGA 5 cut(s) 226, 308, 677, 896, 1156
AsuII TTCGAA 1 cut(s) 1131
AvaI CYCGRG 3 cut(s) 23, 365, 650
AvaII GGWCC 1 cut(s) 420
BaeGI GKGCMC 2 cut(s) 446, 614
BamHI GGATCC 1 cut(s) 621
BanI GGYRCC 2 cut(s) 457, 1010
BanII GRGCYC 5 cut(s) 221, 446, 490, 614, 657
BarI GAAGNNNNNNTAC 2 cut(s) 702, 734
BbsI GAAGAC 2 cut(s) 441, 1140
Bbv12I GWGCWC 3 cut(s) 69, 221, 490
BbvCI CCTCAGC 1 cut(s) 939
BbvI GCAGC 8 cut(s) 252, 255, 258, 261, 291, 319, 533, 790
BccI CCATC 2 cut(s) 39, 909
BceAI ACGGC 3 cut(s) 463, 607, 944
BcgI CGANNNNNNTGC 2 cut(s) 282, 316
BciT130I CCWGG 2 cut(s) 14, 455
BcnI CCSGG 5 cut(s) 274, 382, 526, 651, 652
BcoDI GTCTC 2 cut(s) 221, 390
BfmI CTRYAG 1 cut(s) 305
BfoI RGCGCY 1 cut(s) 946
BfuAI ACCTGC 2 cut(s) 520, 1064
BglI GCCNNNNNGGC 1 cut(s) 441
BlpI GCTNAGC 2 cut(s) 732, 750
Bme1390I CCNGG 7 cut(s) 14, 274, 382, 455, 526, 651, 652
Bme18I GGWCC 1 cut(s) 420
BmeT110I CYCGRG 3 cut(s) 23, 365, 650
BmgT120I GGNCC 7 cut(s) 187, 420, 442, 443, 610, 611, 647
BmiI GGNNCC 7 cut(s) 444, 459, 612, 623, 649, 1012, 1179
BmrFI CCNGG 7 cut(s) 14, 274, 382, 455, 526, 651, 652
BmrI ACTGGG 2 cut(s) 385, 621
BmsI GCATC 1 cut(s) 812
BmuI ACTGGG 2 cut(s) 385, 621
BpiI GAAGAC 2 cut(s) 441, 1140
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BpmI CTGGAG 1 cut(s) 1164
Bpu10I CCTNAGC 2 cut(s) 939, 1124
Bpu1102I GCTNAGC 2 cut(s) 732, 750
Bpu14I TTCGAA 1 cut(s) 1131
BpuMI CCSGG 5 cut(s) 274, 382, 526, 651, 652
BsaHI GRCGYC 2 cut(s) 60, 476
BsaI GGTCTC 2 cut(s) 221, 390
BsaWI WCCGGW 1 cut(s) 1169
Bsc4I CCNNNNNNNGG 2 cut(s) 370, 1187
Bse118I RCCGGY 3 cut(s) 1013, 1096, 1169
Bse1I ACTGG 2 cut(s) 391, 627
BseBI CCWGG 2 cut(s) 14, 455
BseLI CCNNNNNNNGG 2 cut(s) 370, 1187
BseMII CTCAG 8 cut(s) 392, 503, 657, 692, 746, 764, 953, 1115
BseNI ACTGG 2 cut(s) 391, 627
BseRI GAGGAG 1 cut(s) 272
BseSI GKGCMC 2 cut(s) 446, 614
BseX3I CGGCCG 1 cut(s) 592
BseXI GCAGC 8 cut(s) 252, 255, 258, 261, 291, 319, 533, 790
BseYI CCCAGC 1 cut(s) 762
Bsh1236I CGCG 5 cut(s) 132, 518, 538, 597, 1159
Bsh1285I CGRYCG 1 cut(s) 595
BshFI GGCC 7 cut(s) 188, 444, 594, 612, 648, 859, 1100
BshNI GGYRCC 2 cut(s) 457, 1010
BshTI ACCGGT 1 cut(s) 1169
BsiEI CGRYCG 1 cut(s) 595
BsiHKAI GWGCWC 3 cut(s) 69, 221, 490
BsiHKCI CYCGRG 3 cut(s) 23, 365, 650
BsiSI CCGG 8 cut(s) 112, 273, 381, 525, 651, 1014, 1097, 1170
BslFI GGGAC 2 cut(s) 323, 951
BslI CCNNNNNNNGG 2 cut(s) 370, 1187
BsmAI GTCTC 2 cut(s) 221, 390
BsmFI GGGAC 2 cut(s) 323, 951
BsnI GGCC 7 cut(s) 188, 444, 594, 612, 648, 859, 1100
Bso31I GGTCTC 2 cut(s) 221, 390
BsoBI CYCGRG 3 cut(s) 23, 365, 650
Bsp119I TTCGAA 1 cut(s) 1131
Bsp120I GGGCCC 2 cut(s) 442, 610
Bsp1286I GDGCHC 6 cut(s) 69, 221, 446, 490, 614, 657
Bsp143I GATC 3 cut(s) 19, 621, 783
Bsp1720I GCTNAGC 2 cut(s) 732, 750
Bsp19I CCATGG 2 cut(s) 722, 1186
BspANI GGCC 7 cut(s) 188, 444, 594, 612, 648, 859, 1100
BspCNI CTCAG 8 cut(s) 393, 502, 656, 691, 745, 763, 952, 1116
BspFNI CGCG 5 cut(s) 132, 518, 538, 597, 1159
BspLI GGNNCC 7 cut(s) 444, 459, 612, 623, 649, 1012, 1179
BspMAI CTGCAG 1 cut(s) 309
BspMI ACCTGC 2 cut(s) 520, 1064
BspPI GGATC 4 cut(s) 14, 616, 629, 778
BspT104I TTCGAA 1 cut(s) 1131
BspT107I GGYRCC 2 cut(s) 457, 1010
BspTNI GGTCTC 2 cut(s) 221, 390
BsrBI CCGCTC 1 cut(s) 405
BsrFI RCCGGY 3 cut(s) 1013, 1096, 1169
BsrI ACTGG 2 cut(s) 391, 627
BssAI RCCGGY 3 cut(s) 1013, 1096, 1169
BssMI GATC 3 cut(s) 19, 621, 783
BssNI GRCGYC 2 cut(s) 60, 476
BssT1I CCWWGG 3 cut(s) 722, 1101, 1186
Bst2UI CCWGG 2 cut(s) 14, 455
Bst4CI ACNGT 3 cut(s) 297, 983, 1087
BstACI GRCGYC 2 cut(s) 60, 476
BstBI TTCGAA 1 cut(s) 1131
BstC8I GCNNGC 4 cut(s) 312, 827, 1009, 1098
BstDEI CTNAG 8 cut(s) 401, 489, 643, 678, 732, 750, 939, 1124
BstDSI CCRYGG 5 cut(s) 210, 589, 595, 722, 1186
BstFNI CGCG 5 cut(s) 132, 518, 538, 597, 1159
BstH2I RGCGCY 1 cut(s) 946
BstHHI GCGC 2 cut(s) 538, 945
BstKTI GATC 3 cut(s) 22, 624, 786
BstMAI GTCTC 2 cut(s) 221, 390
BstMBI GATC 3 cut(s) 19, 621, 783
BstMCI CGRYCG 1 cut(s) 595
BstNI CCWGG 2 cut(s) 14, 455
BstSCI CCNGG 7 cut(s) 12, 272, 380, 453, 524, 649, 650
BstSFI CTRYAG 1 cut(s) 305
BstSLI GKGCMC 2 cut(s) 446, 614
BstUI CGCG 5 cut(s) 132, 518, 538, 597, 1159
BstV1I GCAGC 8 cut(s) 252, 255, 258, 261, 291, 319, 533, 790
BstV2I GAAGAC 2 cut(s) 441, 1140
BstX2I RGATCY 2 cut(s) 621, 783
BstXI CCANNNNNNTGG 1 cut(s) 1174
BstYI RGATCY 2 cut(s) 621, 783
BstZI CGGCCG 1 cut(s) 592
BsuRI GGCC 7 cut(s) 188, 444, 594, 612, 648, 859, 1100
BtgI CCRYGG 5 cut(s) 210, 589, 595, 722, 1186
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 70
BveI ACCTGC 2 cut(s) 520, 1064
Cac8I GCNNGC 4 cut(s) 312, 827, 1009, 1098
CaiI CAGNNNCTG 1 cut(s) 1117
CfoI GCGC 2 cut(s) 538, 945
Cfr10I RCCGGY 3 cut(s) 1013, 1096, 1169
Cfr13I GGNCC 7 cut(s) 187, 420, 442, 443, 610, 611, 647
Cfr42I CCGCGG 1 cut(s) 598
Cfr9I CCCGGG 1 cut(s) 650
CseI GACGC 4 cut(s) 109, 121, 484, 953
Csp6I GTAC 1 cut(s) 814
CspAI ACCGGT 1 cut(s) 1169
CviAII CATG 8 cut(s) 79, 376, 689, 723, 830, 855, 1138, 1187
CviQI GTAC 1 cut(s) 814
DdeI CTNAG 8 cut(s) 401, 489, 643, 678, 732, 750, 939, 1124
DpnI GATC 3 cut(s) 21, 623, 785
DpnII GATC 3 cut(s) 19, 621, 783
DraIII CACNNNGTG 1 cut(s) 691
DrdI GACNNNNNNGTC 1 cut(s) 445
DseDI GACNNNNNNGTC 1 cut(s) 445
EaeI YGGCCR 3 cut(s) 592, 857, 1098
EagI CGGCCG 1 cut(s) 592
EciI GGCGGA 3 cut(s) 98, 579, 1143
Ecl136II GAGCTC 2 cut(s) 219, 488
EclXI CGGCCG 1 cut(s) 592
Eco130I CCWWGG 3 cut(s) 722, 1101, 1186
Eco24I GRGCYC 5 cut(s) 221, 446, 490, 614, 657
Eco31I GGTCTC 2 cut(s) 221, 390
Eco47I GGWCC 1 cut(s) 420
Eco52I CGGCCG 1 cut(s) 592
Eco53kI GAGCTC 2 cut(s) 219, 488
Eco88I CYCGRG 3 cut(s) 23, 365, 650
EcoICRI GAGCTC 2 cut(s) 219, 488
EcoO109I RGGNCCY 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 12, 453
EcoT14I CCWWGG 3 cut(s) 722, 1101, 1186
EcoT38I GRGCYC 5 cut(s) 221, 446, 490, 614, 657
ErhI CCWWGG 3 cut(s) 722, 1101, 1186
FaeI CATG 8 cut(s) 82, 379, 692, 726, 833, 858, 1141, 1190
FaiI YATR 9 cut(s) 80, 377, 514, 690, 724, 831, 856, 1139, 1188
FaqI GGGAC 2 cut(s) 323, 951
FatI CATG 8 cut(s) 78, 375, 688, 722, 829, 854, 1137, 1186
FauI CCCGC 3 cut(s) 137, 290, 349
FblI GTMKAC 1 cut(s) 395
FriOI GRGCYC 5 cut(s) 221, 446, 490, 614, 657
GlaI GCGC 2 cut(s) 537, 944
GsaI CCCAGC 1 cut(s) 766
GsuI CTGGAG 1 cut(s) 1164
HaeII RGCGCY 1 cut(s) 946
HaeIII GGCC 7 cut(s) 188, 444, 594, 612, 648, 859, 1100
HapII CCGG 8 cut(s) 112, 273, 381, 525, 651, 1014, 1097, 1170
HgaI GACGC 4 cut(s) 109, 121, 484, 953
HhaI GCGC 2 cut(s) 538, 945
Hin1I GRCGYC 2 cut(s) 60, 476
Hin1II CATG 8 cut(s) 82, 379, 692, 726, 833, 858, 1141, 1190
Hin6I GCGC 2 cut(s) 536, 943
HinP1I GCGC 2 cut(s) 536, 943
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 670
HinfI GANTC 7 cut(s) 4, 125, 167, 176, 206, 397, 408
HpaII CCGG 8 cut(s) 112, 273, 381, 525, 651, 1014, 1097, 1170
HphI GGTGA 5 cut(s) 226, 308, 677, 896, 1156
Hpy166II GTNNAC 2 cut(s) 396, 602
Hpy188I TCNGA 5 cut(s) 175, 402, 492, 621, 1119
Hpy188III TCNNGA 5 cut(s) 23, 412, 994, 1055, 1181
Hpy8I GTNNAC 2 cut(s) 396, 602
Hpy99I CGWCG 7 cut(s) 62, 65, 125, 257, 442, 475, 478
HpyAV CCTTC 3 cut(s) 358, 471, 1056
HpyCH4III ACNGT 3 cut(s) 297, 983, 1087
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 2 cut(s) 75, 307
HpyF3I CTNAG 8 cut(s) 401, 489, 643, 678, 732, 750, 939, 1124
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 2 cut(s) 60, 476
Hsp92II CATG 8 cut(s) 82, 379, 692, 726, 833, 858, 1141, 1190
HspAI GCGC 2 cut(s) 536, 943
KroI GCCGGC 1 cut(s) 1096
KroNI GCCGGC 1 cut(s) 1098
KspI CCGCGG 1 cut(s) 598
Kzo9I GATC 3 cut(s) 19, 621, 783
LmnI GCTCC 9 cut(s) 72, 224, 414, 485, 505, 566, 759, 1156, 1183
Lsp1109I GCAGC 8 cut(s) 252, 255, 258, 261, 291, 319, 533, 790
LweI GCATC 1 cut(s) 812
MaeII ACGT 1 cut(s) 60
MalI GATC 3 cut(s) 21, 623, 785
MbiI CCGCTC 1 cut(s) 405
MboI GATC 3 cut(s) 19, 621, 783
MboII GAAGA 4 cut(s) 441, 710, 810, 1145
MflI RGATCY 2 cut(s) 621, 783
MhlI GDGCHC 6 cut(s) 69, 221, 446, 490, 614, 657
MluCI AATT 2 cut(s) 838, 1192
MlyI GAGTC 4 cut(s) 161, 170, 215, 391
MmeI TCCRAC 2 cut(s) 198, 898
MroNI GCCGGC 1 cut(s) 1096
MseI TTAA 3 cut(s) 542, 837, 1195
MspA1I CMGCKG 1 cut(s) 597
MspI CCGG 8 cut(s) 112, 273, 381, 525, 651, 1014, 1097, 1170
MspR9I CCNGG 7 cut(s) 14, 274, 382, 455, 526, 651, 652
MvaI CCWGG 2 cut(s) 14, 455
MvnI CGCG 5 cut(s) 132, 518, 538, 597, 1159
NaeI GCCGGC 1 cut(s) 1098
NciI CCSGG 5 cut(s) 274, 382, 526, 651, 652
NcoI CCATGG 2 cut(s) 722, 1186
NdeII GATC 3 cut(s) 19, 621, 783
NgoMIV GCCGGC 1 cut(s) 1096
NlaIII CATG 8 cut(s) 82, 379, 692, 726, 833, 858, 1141, 1190
NlaIV GGNNCC 7 cut(s) 444, 459, 612, 623, 649, 1012, 1179
NmeAIII GCCGAG 2 cut(s) 164, 540
NspV TTCGAA 1 cut(s) 1131
PcsI WCGNNNNNNNCGW 4 cut(s) 129, 171, 470, 822
PdiI GCCGGC 1 cut(s) 1098
PfeI GAWTC 3 cut(s) 4, 125, 408
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 1187
PinAI ACCGGT 1 cut(s) 1169
PleI GAGTC 4 cut(s) 161, 170, 214, 391
PpsI GAGTC 4 cut(s) 161, 170, 214, 391
Psp124BI GAGCTC 2 cut(s) 221, 490
Psp6I CCWGG 2 cut(s) 12, 453
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 2 cut(s) 12, 453
PspN4I GGNNCC 7 cut(s) 444, 459, 612, 623, 649, 1012, 1179
PspOMI GGGCCC 2 cut(s) 442, 610
PspPI GGNCC 7 cut(s) 187, 420, 442, 443, 610, 611, 647
PstI CTGCAG 1 cut(s) 309
PstNI CAGNNNCTG 1 cut(s) 1117
PsuI RGATCY 2 cut(s) 621, 783
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 1 cut(s) 815
RsaNI GTAC 1 cut(s) 814
SacI GAGCTC 2 cut(s) 221, 490
SacII CCGCGG 1 cut(s) 598
SalI GTCGAC 1 cut(s) 394
SaqAI TTAA 3 cut(s) 542, 837, 1195
Sau3AI GATC 3 cut(s) 19, 621, 783
Sau96I GGNCC 7 cut(s) 187, 420, 442, 443, 610, 611, 647
SchI GAGTC 4 cut(s) 161, 170, 215, 391
ScrFI CCNGG 7 cut(s) 14, 274, 382, 455, 526, 651, 652
SduI GDGCHC 6 cut(s) 69, 221, 446, 490, 614, 657
SfaNI GCATC 1 cut(s) 812
SfcI CTRYAG 1 cut(s) 305
Sfr303I CCGCGG 1 cut(s) 598
SfuI TTCGAA 1 cut(s) 1131
SgrAI CRCCGGYG 1 cut(s) 1013
SgrBI CCGCGG 1 cut(s) 598
SinI GGWCC 1 cut(s) 420
SmaI CCCGGG 1 cut(s) 652
Sse9I AATT 2 cut(s) 838, 1192
SstI GAGCTC 2 cut(s) 221, 490
StyD4I CCNGG 7 cut(s) 12, 272, 380, 453, 524, 649, 650
StyI CCWWGG 3 cut(s) 722, 1101, 1186
TaaI ACNGT 3 cut(s) 297, 983, 1087
TaiI ACGT 1 cut(s) 63
TaqI TCGA 7 cut(s) 123, 165, 204, 395, 662, 851, 1131
TaqII GACCGA 1 cut(s) 245
TasI AATT 2 cut(s) 838, 1192
TauI GCSGC 9 cut(s) 288, 347, 521, 585, 597, 862, 865, 959, 1159
TfiI GAWTC 3 cut(s) 4, 125, 408
Tru1I TTAA 3 cut(s) 542, 837, 1195
Tru9I TTAA 3 cut(s) 542, 837, 1195
TscAI CASTG 1 cut(s) 77
TseI GCWGC 8 cut(s) 240, 243, 246, 249, 304, 307, 521, 778
TspDTI ATGAA 2 cut(s) 17, 1154
TspGWI ACGGA 4 cut(s) 68, 199, 325, 444
TspMI CCCGGG 1 cut(s) 650
TspRI CASTG 1 cut(s) 77
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 1 cut(s) 1187
VpaK11BI GGWCC 1 cut(s) 420
XcmI CCANNNNNNNNNTGG 1 cut(s) 596
XmaI CCCGGG 1 cut(s) 650
XmiI GTMKAC 1 cut(s) 395
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.