Rorug01G0345700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0011808
Physical Location & Seq
Reverse (-)
46309055 .. 46311852
2798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0345700.1

Sequence Viewer

Length: 1344 bp
ATGGGCAAGGAAAAGTTTCATATCAACATTGTGGTTATTGGCCATGTTGACTCTGGGAAGTCGACCACCACTGGTCATTTGATCTACAAGCTTGGGGGTATTGACAAGCGTGTGATTGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGCGTTCATTCAAGTATGCTTGGGTGCTTGACAAGCTTAAGGCTGAGCGTGAAAGGGGTATTACCATTGACATTGCTCTCTGGAAGTTTGAGACCACCAAGTACTACTGCACTGTCATTGATGCTCCTGGACATCGTGACTTCATCAAGAACATGATTACTGGAACTTCCCAGGCGGATTGTGCTGTCCTCATTATTGACTCTACCACTGGAGGTTTTGAGGCTGGTATCTCGAAGGATGGTCAGACCCGTGAGCATGCTCTCCTTGCTTTCACCCTCGGTGTTAGGCAAATGATTTGTTGCTGCAACAAGATGGATGCTACCTCTCCTAAGTACTCGAAGGCAAGGTATGATGAAATCGTGAAGGAAGTGTCATCCTATCTGAAGAAGATTGGATACAACCCAGAAAAAATTGCTTTTGTTCCCATCTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTCGACTGGTACAAGGGTCCAACCCTTCTTGAGGCCCTTGACCTGATCAACGAGCCCAAGAGGCCCTCCGATAAGCCCCTCCGACTTCCACTTCAGGATGTCTACAAGATTGGAGGTATTGGAACTGTTCCTGTTGGACGTGTTGAGACTGGTATTATCAAGCCTGGTATGGTTGTGACTTTTGGTCCCACTGGATTGACCACTGAAGTTAAGTCTGTGGAGATGCACCACGAGGCTCTCCAGGAGGCTCTTCCTGGTGACAATGTTGGCTTCAACGTCAAGAACGTTGCAGTCAAGGATCTCAAGCGTGGGTTTGTTGCTTCAAACTCCAAGGATGATCCAGCCAAGGAAGCAGCTAACTTCACTGCCCAGGTCATCATTATGAATCACCCTGGCCAGATTGGAAACGGTTATGCTCCTGTGCTCGATTGCCACACCTCTCACATTGCTGTCAAGTTCAAAGAGATTTTGACCAAGATTGACAGACGTTCTGGCAAGGAGCTAGAGAAGGAGCCCAAGTTTTTGAAGAATGGTGACGCAGGGTTTGTTAAGATGATTCCCACCAAGCCCATGGTCGTTGAAACTTTCTCCGAGTATCCCCCACTTGGTCGTTTTGCTGTGAGGGACATGCGTCAGACAGTTGCTGTGGGAGTCATCAAGGCTGTGGAGAAGAAGGATCCCAGCGGAGCCAAGGTTACCAAGTCTGCGGTCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

447

Amino Acids

49.44

Weight (kDa)

9.2

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 221 3.3e-57 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 248 - 313 2.7e-17 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 322 - 429 1.1e-27 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 327 - 427 3.3e-38 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 62, 723
AciI CCGC 3 cut(s) 326, 1305, 1328
AclI AACGTT 1 cut(s) 906
AclWI GGATC 4 cut(s) 927, 953, 1292, 1305
AcoI YGGCCR 2 cut(s) 40, 1015
AcuI CTGAAG 3 cut(s) 554, 698, 846
AfaI GTAC 3 cut(s) 254, 485, 632
AfiI CCNNNNNNNGG 3 cut(s) 581, 652, 1226
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 6 cut(s) 163, 895, 945, 1081, 1147, 1202
AhdI GACNNNNNGTC 1 cut(s) 804
AjiI CACGTC 1 cut(s) 761
AjnI CCWGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
AluBI AGCT 4 cut(s) 91, 187, 977, 1123
AluI AGCT 4 cut(s) 91, 187, 977, 1123
Alw21I GWGCWC 1 cut(s) 1047
Alw26I GTCTC 2 cut(s) 236, 761
AlwI GGATC 4 cut(s) 927, 953, 1292, 1305
AlwNI CAGNNNCTG 1 cut(s) 1265
AoxI GGCC 4 cut(s) 40, 654, 683, 1015
ApeKI GCWGC 3 cut(s) 137, 453, 974
ArsI GACNNNNNNTTYG 2 cut(s) 1148, 1180
Asp700I GAANNNNTTC 1 cut(s) 15
AspS9I GGNCC 5 cut(s) 612, 638, 655, 684, 806
AsuHPI GGTGA 5 cut(s) 415, 605, 890, 1001, 1166
AvaII GGWCC 3 cut(s) 612, 638, 806
BaeI ACNNNNGTAYC 2 cut(s) 622, 655
BalI TGGCCA 2 cut(s) 42, 1017
BamHI GGATCC 1 cut(s) 1297
BanII GRGCYC 2 cut(s) 678, 1137
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BauI CACGAG 1 cut(s) 851
Bbv12I GWGCWC 1 cut(s) 1047
BbvI GCAGC 3 cut(s) 124, 440, 986
BccI CCATC 3 cut(s) 383, 457, 584
BciT130I CCWGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
BciVI GTATCC 2 cut(s) 539, 1227
BclI TGATCA 1 cut(s) 666
BcoDI GTCTC 2 cut(s) 236, 761
BfaI CTAG 1 cut(s) 1124
BfrI CTTAAG 1 cut(s) 188
BfuI GTATCC 2 cut(s) 539, 1227
BglI GCCNNNNNGGC 1 cut(s) 682
BisI GCNGC 3 cut(s) 138, 454, 975
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 3 cut(s) 139, 455, 976
BmcAI AGTACT 2 cut(s) 254, 485
Bme1390I CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
Bme18I GGWCC 3 cut(s) 612, 638, 806
BmeRI GACNNNNNGTC 1 cut(s) 804
BmgBI CACGTC 1 cut(s) 761
BmgT120I GGNCC 5 cut(s) 612, 638, 655, 684, 806
BmiI GGNNCC 5 cut(s) 639, 808, 1134, 1299, 1309
BmrFI CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
BmsI GCATC 3 cut(s) 262, 457, 834
BoxI GACNNNNGTC 1 cut(s) 1251
BpmI CTGGAG 2 cut(s) 381, 845
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 2 cut(s) 671, 908
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 8 cut(s) 321, 427, 951, 966, 990, 1012, 1191, 1311
BsaXI ACNNNNNCTCC 4 cut(s) 1124, 1154, 1299, 1329
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 652, 1226
Bse1I ACTGG 6 cut(s) 76, 316, 364, 632, 775, 817
Bse3DI GCAATG 2 cut(s) 222, 1065
BseBI CCWGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
BseDI CCNNGG 8 cut(s) 321, 427, 951, 966, 990, 1012, 1191, 1311
BseGI GGATG 5 cut(s) 394, 472, 524, 724, 961
BseLI CCNNNNNNNGG 3 cut(s) 581, 652, 1226
BseMI GCAATG 2 cut(s) 222, 1065
BseMII CTCAG 2 cut(s) 132, 186
BseNI ACTGG 6 cut(s) 76, 316, 364, 632, 775, 817
BseXI GCAGC 3 cut(s) 124, 440, 986
BseYI CCCAGC 1 cut(s) 1301
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 4 cut(s) 42, 656, 685, 1017
BsiHKAI GWGCWC 1 cut(s) 1047
BslFI GGGAC 2 cut(s) 792, 1259
BslI CCNNNNNNNGG 3 cut(s) 581, 652, 1226
BsmAI GTCTC 2 cut(s) 236, 761
BsmFI GGGAC 2 cut(s) 792, 1259
BsnI GGCC 4 cut(s) 42, 656, 685, 1017
Bso31I GGTCTC 1 cut(s) 236
Bsp1286I GDGCHC 3 cut(s) 678, 1047, 1137
Bsp143I GATC 5 cut(s) 81, 666, 919, 958, 1297
Bsp1720I GCTNAGC 1 cut(s) 195
Bsp19I CCATGG 1 cut(s) 1191
BspACI CCGC 3 cut(s) 326, 1305, 1328
BspANI GGCC 4 cut(s) 42, 656, 685, 1017
BspCNI CTCAG 2 cut(s) 133, 187
BspLI GGNNCC 5 cut(s) 639, 808, 1134, 1299, 1309
BspPI GGATC 4 cut(s) 927, 953, 1292, 1305
BspQI GCTCTTC 1 cut(s) 876
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 2 cut(s) 222, 1065
BsrI ACTGG 6 cut(s) 76, 316, 364, 632, 775, 817
BssECI CCNNGG 8 cut(s) 321, 427, 951, 966, 990, 1012, 1191, 1311
BssMI GATC 5 cut(s) 81, 666, 919, 958, 1297
BssSI CACGAG 1 cut(s) 851
BssT1I CCWWGG 4 cut(s) 951, 966, 1191, 1311
Bst2BI CACGAG 1 cut(s) 851
Bst2UI CCWGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
Bst4CI ACNGT 4 cut(s) 265, 748, 1031, 1261
Bst6I CTCTTC 1 cut(s) 876
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 3 cut(s) 141, 195, 480
BstDSI CCRYGG 1 cut(s) 1191
BstEII GGTNACC 1 cut(s) 1315
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 5 cut(s) 394, 472, 524, 724, 961
BstKTI GATC 5 cut(s) 84, 669, 922, 961, 1300
BstMAI GTCTC 2 cut(s) 236, 761
BstMBI GATC 5 cut(s) 81, 666, 919, 958, 1297
BstMWI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
BstNI CCWGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
BstNSI RCATGY 2 cut(s) 410, 1252
BstPAI GACNNNNGTC 1 cut(s) 1251
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
BstV1I GCAGC 3 cut(s) 124, 440, 986
BstX2I RGATCY 2 cut(s) 919, 1297
BstXI CCANNNNNNTGG 1 cut(s) 1192
BstYI RGATCY 2 cut(s) 919, 1297
BsuI GTATCC 2 cut(s) 539, 1227
BsuRI GGCC 4 cut(s) 42, 656, 685, 1017
BtgI CCRYGG 1 cut(s) 1191
BtrI CACGTC 1 cut(s) 761
BtsCI GGATG 5 cut(s) 394, 472, 524, 724, 961
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 6 cut(s) 69, 261, 357, 810, 822, 984
Cac8I GCNNGC 1 cut(s) 408
CaiI CAGNNNCTG 1 cut(s) 1265
Cfr13I GGNCC 5 cut(s) 612, 638, 655, 684, 806
CseI GACGC 2 cut(s) 1166, 1241
Csp6I GTAC 3 cut(s) 253, 484, 631
CviAII CATG 6 cut(s) 44, 304, 407, 601, 1192, 1249
CviQI GTAC 3 cut(s) 253, 484, 631
DdeI CTNAG 3 cut(s) 141, 195, 480
DpnI GATC 5 cut(s) 83, 668, 921, 960, 1299
DpnII GATC 5 cut(s) 81, 666, 919, 958, 1297
DriI GACNNNNNGTC 1 cut(s) 804
EaeI YGGCCR 2 cut(s) 40, 1015
Eam1104I CTCTTC 1 cut(s) 876
Eam1105I GACNNNNNGTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 876
EciI GGCGGA 1 cut(s) 341
Eco130I CCWWGG 4 cut(s) 951, 966, 1191, 1311
Eco24I GRGCYC 2 cut(s) 678, 1137
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 3 cut(s) 612, 638, 806
Eco57I CTGAAG 3 cut(s) 554, 698, 846
Eco91I GGTNACC 1 cut(s) 1315
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO109I RGGNCCY 2 cut(s) 655, 684
EcoO65I GGTNACC 1 cut(s) 1315
EcoRII CCWGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
EcoT14I CCWWGG 4 cut(s) 951, 966, 1191, 1311
EcoT38I GRGCYC 2 cut(s) 678, 1137
ErhI CCWWGG 4 cut(s) 951, 966, 1191, 1311
FaeI CATG 6 cut(s) 47, 307, 410, 604, 1195, 1252
FaqI GGGAC 2 cut(s) 792, 1259
FatI CATG 6 cut(s) 43, 303, 406, 600, 1191, 1248
FbaI TGATCA 1 cut(s) 666
FblI GTMKAC 2 cut(s) 62, 723
Fnu4HI GCNGC 3 cut(s) 138, 454, 975
FokI GGATG 5 cut(s) 401, 479, 511, 731, 968
FriOI GRGCYC 2 cut(s) 678, 1137
Fsp4HI GCNGC 3 cut(s) 138, 454, 975
FspBI CTAG 1 cut(s) 1124
GluI GCNGC 3 cut(s) 138, 454, 975
GsaI CCCAGC 1 cut(s) 1305
GsuI CTGGAG 2 cut(s) 381, 845
HaeIII GGCC 4 cut(s) 42, 656, 685, 1017
HgaI GACGC 2 cut(s) 1166, 1241
Hin1II CATG 6 cut(s) 47, 307, 410, 604, 1195, 1252
HincII GTYRAC 2 cut(s) 49, 63
HindII GTYRAC 2 cut(s) 49, 63
HindIII AAGCTT 2 cut(s) 89, 185
HinfI GANTC 5 cut(s) 50, 350, 1006, 1177, 1272
HphI GGTGA 5 cut(s) 415, 605, 890, 1001, 1166
Hpy166II GTNNAC 4 cut(s) 49, 63, 615, 724
Hpy188I TCNGA 6 cut(s) 396, 534, 691, 704, 1213, 1257
Hpy8I GTNNAC 4 cut(s) 49, 63, 615, 724
HpyAV CCTTC 7 cut(s) 124, 379, 484, 508, 656, 1123, 1288
HpyCH4III ACNGT 4 cut(s) 265, 748, 1031, 1261
HpyCH4IV ACGT 4 cut(s) 760, 897, 906, 1108
HpyCH4V TGCA 4 cut(s) 261, 456, 847, 911
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
HpyF3I CTNAG 3 cut(s) 141, 195, 480
HpySE526I ACGT 4 cut(s) 760, 897, 906, 1108
Hsp92II CATG 6 cut(s) 47, 307, 410, 604, 1195, 1252
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 5 cut(s) 81, 666, 919, 958, 1297
LguI GCTCTTC 1 cut(s) 876
LmnI GCTCC 5 cut(s) 280, 1042, 1120, 1132, 1307
Lsp1109I GCAGC 3 cut(s) 124, 440, 986
LweI GCATC 3 cut(s) 262, 457, 834
MaeI CTAG 1 cut(s) 1124
MaeII ACGT 4 cut(s) 760, 897, 906, 1108
MaeIII GTNAC 6 cut(s) 287, 593, 796, 878, 1154, 1315
MalI GATC 5 cut(s) 83, 668, 921, 960, 1299
MboI GATC 5 cut(s) 81, 666, 919, 958, 1297
MboII GAAGA 5 cut(s) 547, 550, 863, 1159, 1303
MflI RGATCY 2 cut(s) 919, 1297
MhlI GDGCHC 3 cut(s) 678, 1047, 1137
MlsI TGGCCA 2 cut(s) 42, 1017
MluCI AATT 1 cut(s) 561
MluNI TGGCCA 2 cut(s) 42, 1017
MlyI GAGTC 3 cut(s) 44, 344, 1281
MmeI TCCRAC 3 cut(s) 665, 727, 736
Mox20I TGGCCA 2 cut(s) 42, 1017
MroXI GAANNNNTTC 1 cut(s) 15
MscI TGGCCA 2 cut(s) 42, 1017
MseI TTAA 3 cut(s) 189, 831, 1170
MslI CAYNNNNRTG 1 cut(s) 1001
Msp20I TGGCCA 2 cut(s) 42, 1017
MspA1I CMGCKG 1 cut(s) 1305
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
MvaI CCWGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
MwoI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
NcoI CCATGG 1 cut(s) 1191
NdeII GATC 5 cut(s) 81, 666, 919, 958, 1297
NlaIII CATG 6 cut(s) 47, 307, 410, 604, 1195, 1252
NlaIV GGNNCC 5 cut(s) 639, 808, 1134, 1299, 1309
NmuCI GTSAC 5 cut(s) 287, 593, 796, 878, 1154
NspI RCATGY 2 cut(s) 410, 1252
PaeI GCATGC 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 876
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 1006, 1177
PfoI TCCNGGA 2 cut(s) 277, 861
PkrI GCNGC 3 cut(s) 139, 455, 976
PleI GAGTC 3 cut(s) 44, 344, 1280
PpsI GAGTC 3 cut(s) 44, 344, 1280
PshAI GACNNNNGTC 1 cut(s) 1251
Psp1406I AACGTT 1 cut(s) 906
Psp6I CCWGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
PspEI GGTNACC 1 cut(s) 1315
PspFI CCCAGC 1 cut(s) 1301
PspGI CCWGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
PspN4I GGNNCC 5 cut(s) 639, 808, 1134, 1299, 1309
PspPI GGNCC 5 cut(s) 612, 638, 655, 684, 806
PstNI CAGNNNCTG 1 cut(s) 1265
PsuI RGATCY 2 cut(s) 919, 1297
RsaI GTAC 3 cut(s) 254, 485, 632
RsaNI GTAC 3 cut(s) 253, 484, 631
RseI CAYNNNNRTG 1 cut(s) 1001
SalI GTCGAC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 3 cut(s) 189, 831, 1170
SatI GCNGC 3 cut(s) 138, 454, 975
Sau3AI GATC 5 cut(s) 81, 666, 919, 958, 1297
Sau96I GGNCC 5 cut(s) 612, 638, 655, 684, 806
ScaI AGTACT 2 cut(s) 254, 485
SchI GAGTC 3 cut(s) 44, 344, 1281
ScrFI CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
SduI GDGCHC 3 cut(s) 678, 1047, 1137
SfaNI GCATC 3 cut(s) 262, 457, 834
SinI GGWCC 3 cut(s) 612, 638, 806
SmiMI CAYNNNNRTG 1 cut(s) 1001
SmlI CTYRAG 3 cut(s) 188, 650, 923
SmoI CTYRAG 3 cut(s) 188, 650, 923
SphI GCATGC 1 cut(s) 410
Sse9I AATT 1 cut(s) 561
SsiI CCGC 3 cut(s) 326, 1305, 1328
SspMI CTAG 1 cut(s) 1124
StyD4I CCNGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
StyI CCWWGG 4 cut(s) 951, 966, 1191, 1311
TaaI ACNGT 4 cut(s) 265, 748, 1031, 1261
TaiI ACGT 4 cut(s) 763, 900, 909, 1111
TaqI TCGA 6 cut(s) 62, 126, 383, 488, 624, 1047
TasI AATT 1 cut(s) 561
TatI WGTACW 2 cut(s) 252, 483
TfiI GAWTC 2 cut(s) 1006, 1177
Tru1I TTAA 3 cut(s) 189, 831, 1170
Tru9I TTAA 3 cut(s) 189, 831, 1170
TscAI CASTG 6 cut(s) 76, 268, 364, 817, 829, 991
TseFI GTSAC 5 cut(s) 287, 593, 796, 878, 1154
TseI GCWGC 3 cut(s) 137, 453, 974
Tsp45I GTSAC 5 cut(s) 287, 593, 796, 878, 1154
TspDTI ATGAA 6 cut(s) 8, 147, 161, 283, 519, 1019
TspRI CASTG 6 cut(s) 76, 268, 364, 817, 829, 991
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 3 cut(s) 612, 638, 806
XagI CCTNNNNNAGG 1 cut(s) 650
XceI RCATGY 2 cut(s) 410, 1252
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 1189
XmiI GTMKAC 2 cut(s) 62, 723
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 1 cut(s) 1124
ZrmI AGTACT 2 cut(s) 254, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.