Rmu_sc0030064.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0030064.1
OG0011808
Physical Location & Seq
Forward (+)
1 .. 1205
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0030064.1_g000001.1.cds

Sequence Viewer

Length: 891 bp
cagtcgactctgagcggaatcgggagctggtctagcaacggaagcccggcgggtccttcttcccaggtgccgtctcctccaacgacgccgtttgggggtcaaaacgacacctgggatctgatttacgccgccgccggacaagtggcgaggttgaagatgaacagtgttgaaggagcagccaagttcagtaacaacaacagcaacggcagagggcttctgggcccacctcggagcccaagtccgtctcttccttgcgtccaaaaccaaagccccttcacccagtttcagcaagtgagacaagaccaagtactgcagtcacagagctctgctgcttcttggggaaaacaagtgaagcttaactggtcagctcagcaacaacagcagcagcaacagatccaaaacagaggaagaaatatcggtctgtatgaaagtggaagatgtgcgcatggtgtgagtttgccccaatctcaaaatgcatggcctcctcttcaagctcaacaccaaaaccaacaacacccacatcgcaatacccgacccgttttgcctaatgggtctaatgtcaagagggagtctaccggcaccggcgtcttcttgcctcgcagatactccaaccccaatccgactcctgagccacctcgcaaaagagcaggttgccctactgttttgctcccagccaaggtggttcaagctctgaacttgagctttgaggacatgaatggtcatcatcaacaacaccatcttcagccacggttcaacaacactgctttggctccaaaccatgaggctctggtggcaagaagaaacgcgctattgactcagcaaagaaggggtttaaggcaagatgggccactaaactatgaagtgcgcttgcctcaggaatggacctactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

296

Amino Acids

32.63

Weight (kDa)

10.71

Isoelectric Point (pI)

63.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 638
AccB1I GGYRCC 2 cut(s) 67, 578
AccBSI CCGCTC 1 cut(s) 15
AccI GTMKAC 2 cut(s) 5, 572
AccII CGCG 1 cut(s) 806
AciI CCGC 4 cut(s) 15, 50, 129, 132
AclWI GGATC 2 cut(s) 123, 388
AcuI CTGAAG 1 cut(s) 725
AcyI GRCGYC 2 cut(s) 86, 585
AfaI GTAC 1 cut(s) 309
AfiI CCNNNNNNNGG 2 cut(s) 95, 676
AgsI TTSAA 5 cut(s) 154, 170, 491, 686, 754
AjnI CCWGG 2 cut(s) 63, 110
AluBI AGCT 7 cut(s) 27, 324, 355, 368, 494, 689, 702
AluI AGCT 7 cut(s) 27, 324, 355, 368, 494, 689, 702
Alw21I GWGCWC 1 cut(s) 326
Alw26I GTCTC 3 cut(s) 78, 249, 289
AlwI GGATC 2 cut(s) 123, 388
AoxI GGCC 3 cut(s) 220, 479, 845
ApaI GGGCCC 1 cut(s) 224
ApeKI GCWGC 4 cut(s) 176, 329, 382, 385
AspLEI GCGC 3 cut(s) 445, 808, 867
AspS9I GGNCC 5 cut(s) 53, 220, 221, 845, 882
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 2 cut(s) 53, 882
AxyI CCTNAGG 1 cut(s) 873
BaeGI GKGCMC 1 cut(s) 224
BanI GGYRCC 2 cut(s) 67, 578
BanII GRGCYC 3 cut(s) 224, 236, 326
BbsI GAAGAC 1 cut(s) 580
Bbv12I GWGCWC 1 cut(s) 326
BbvI GCAGC 4 cut(s) 188, 316, 394, 397
BccI CCATC 2 cut(s) 744, 836
BceAI ACGGC 3 cut(s) 55, 73, 220
BciT130I CCWGG 2 cut(s) 65, 112
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 3 cut(s) 78, 249, 289
BfaI CTAG 1 cut(s) 33
BfmI CTRYAG 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 638
BisI GCNGC 6 cut(s) 129, 132, 177, 330, 383, 386
BlpI GCTNAGC 1 cut(s) 369
BlsI GCNGC 6 cut(s) 130, 133, 178, 331, 384, 387
BmcAI AGTACT 1 cut(s) 309
Bme1390I CCNGG 3 cut(s) 47, 65, 112
Bme18I GGWCC 2 cut(s) 53, 882
BmgT120I GGNCC 5 cut(s) 53, 220, 221, 845, 882
BmiI GGNNCC 6 cut(s) 54, 69, 222, 233, 580, 771
BmrFI CCNGG 3 cut(s) 47, 65, 112
BmrI ACTGGG 1 cut(s) 274
BmuI ACTGGG 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 580
Bpu10I CCTNAGC 1 cut(s) 627
Bpu1102I GCTNAGC 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 718
BpuMI CCSGG 1 cut(s) 47
BsaHI GRCGYC 2 cut(s) 86, 585
BsaJI CCNNGG 5 cut(s) 63, 111, 227, 675, 746
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 676
Bse118I RCCGGY 2 cut(s) 575, 581
Bse1I ACTGG 2 cut(s) 280, 365
Bse21I CCTNAGG 1 cut(s) 873
BseBI CCWGG 2 cut(s) 65, 112
BseDI CCNNGG 5 cut(s) 63, 111, 227, 675, 746
BseLI CCNNNNNNNGG 2 cut(s) 95, 676
BseMII CTCAG 4 cut(s) 383, 618, 830, 887
BseNI ACTGG 2 cut(s) 280, 365
BseRI GAGGAG 2 cut(s) 66, 474
BseSI GKGCMC 1 cut(s) 224
BseXI GCAGC 4 cut(s) 188, 316, 394, 397
BseYI CCCAGC 1 cut(s) 670
Bsh1236I CGCG 1 cut(s) 806
BshFI GGCC 3 cut(s) 222, 481, 847
BshNI GGYRCC 2 cut(s) 67, 578
BsiHKAI GWGCWC 1 cut(s) 326
BsiSI CCGG 4 cut(s) 47, 135, 576, 582
BslI CCNNNNNNNGG 2 cut(s) 95, 676
BsmAI GTCTC 3 cut(s) 78, 249, 289
BsmBI CGTCTC 2 cut(s) 78, 249
BsnI GGCC 3 cut(s) 222, 481, 847
Bsp120I GGGCCC 1 cut(s) 220
Bsp1286I GDGCHC 3 cut(s) 224, 236, 326
Bsp143I GATC 2 cut(s) 115, 393
Bsp1720I GCTNAGC 1 cut(s) 369
BspACI CCGC 4 cut(s) 15, 50, 129, 132
BspANI GGCC 3 cut(s) 222, 481, 847
BspCNI CTCAG 4 cut(s) 382, 619, 829, 886
BspFNI CGCG 1 cut(s) 806
BspLI GGNNCC 6 cut(s) 54, 69, 222, 233, 580, 771
BspMAI CTGCAG 1 cut(s) 315
BspMI ACCTGC 1 cut(s) 638
BspPI GGATC 2 cut(s) 123, 388
BspT107I GGYRCC 2 cut(s) 67, 578
BsrBI CCGCTC 1 cut(s) 15
BsrFI RCCGGY 2 cut(s) 575, 581
BsrI ACTGG 2 cut(s) 280, 365
BssAI RCCGGY 2 cut(s) 575, 581
BssECI CCNNGG 5 cut(s) 63, 111, 227, 675, 746
BssMI GATC 2 cut(s) 115, 393
BssNI GRCGYC 2 cut(s) 86, 585
BssT1I CCWWGG 1 cut(s) 675
Bst2UI CCWGG 2 cut(s) 65, 112
Bst4CI ACNGT 3 cut(s) 164, 661, 750
Bst6I CTCTTC 2 cut(s) 252, 492
BstACI GRCGYC 2 cut(s) 86, 585
BstC8I GCNNGC 1 cut(s) 869
BstDEI CTNAG 5 cut(s) 11, 369, 627, 816, 873
BstDSI CCRYGG 1 cut(s) 746
BstFNI CGCG 1 cut(s) 806
BstHHI GCGC 3 cut(s) 445, 808, 867
BstKTI GATC 2 cut(s) 118, 396
BstMAI GTCTC 3 cut(s) 78, 249, 289
BstMBI GATC 2 cut(s) 115, 393
BstMWI GCNNNNNNNGC 5 cut(s) 33, 42, 379, 791, 844
BstNI CCWGG 2 cut(s) 65, 112
BstSCI CCNGG 3 cut(s) 45, 63, 110
BstSFI CTRYAG 1 cut(s) 311
BstSLI GKGCMC 1 cut(s) 224
BstUI CGCG 1 cut(s) 806
BstV1I GCAGC 4 cut(s) 188, 316, 394, 397
BstV2I GAAGAC 1 cut(s) 580
BstX2I RGATCY 2 cut(s) 115, 393
BstYI RGATCY 2 cut(s) 115, 393
Bsu36I CCTNAGG 1 cut(s) 873
BsuRI GGCC 3 cut(s) 222, 481, 847
BtgI CCRYGG 1 cut(s) 746
BtgZI GCGATG 1 cut(s) 506
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 2 cut(s) 169, 759
BveI ACCTGC 1 cut(s) 638
Cac8I GCNNGC 1 cut(s) 869
CfoI GCGC 3 cut(s) 445, 808, 867
Cfr10I RCCGGY 2 cut(s) 575, 581
Cfr13I GGNCC 5 cut(s) 53, 220, 221, 845, 882
CseI GACGC 3 cut(s) 94, 244, 574
Csp6I GTAC 1 cut(s) 308
CviAII CATG 4 cut(s) 446, 477, 712, 779
CviQI GTAC 1 cut(s) 308
DdeI CTNAG 5 cut(s) 11, 369, 627, 816, 873
DpnI GATC 2 cut(s) 117, 395
DpnII GATC 2 cut(s) 115, 393
Eam1104I CTCTTC 2 cut(s) 252, 492
EarI CTCTTC 2 cut(s) 252, 492
Ecl136II GAGCTC 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 675
Eco24I GRGCYC 3 cut(s) 224, 236, 326
Eco47I GGWCC 2 cut(s) 53, 882
Eco53kI GAGCTC 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 725
Eco81I CCTNAGG 1 cut(s) 873
EcoICRI GAGCTC 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 53
EcoRII CCWGG 2 cut(s) 63, 110
EcoT14I CCWWGG 1 cut(s) 675
EcoT22I ATGCAT 1 cut(s) 478
EcoT38I GRGCYC 3 cut(s) 224, 236, 326
ErhI CCWWGG 1 cut(s) 675
Esp3I CGTCTC 2 cut(s) 78, 249
FaeI CATG 4 cut(s) 449, 480, 715, 782
FaiI YATR 6 cut(s) 426, 447, 478, 713, 780, 858
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FatI CATG 4 cut(s) 445, 476, 711, 778
FauI CCCGC 1 cut(s) 43
FblI GTMKAC 2 cut(s) 5, 572
Fnu4HI GCNGC 6 cut(s) 129, 132, 177, 330, 383, 386
FriOI GRGCYC 3 cut(s) 224, 236, 326
Fsp4HI GCNGC 6 cut(s) 129, 132, 177, 330, 383, 386
FspAI RTGCGCAY 1 cut(s) 444
FspBI CTAG 1 cut(s) 33
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 3 cut(s) 444, 807, 866
GluI GCNGC 6 cut(s) 129, 132, 177, 330, 383, 386
GsaI CCCAGC 1 cut(s) 674
HaeIII GGCC 3 cut(s) 222, 481, 847
HapII CCGG 4 cut(s) 47, 135, 576, 582
HgaI GACGC 3 cut(s) 94, 244, 574
HhaI GCGC 3 cut(s) 445, 808, 867
Hin1I GRCGYC 2 cut(s) 86, 585
Hin1II CATG 4 cut(s) 449, 480, 715, 782
Hin6I GCGC 3 cut(s) 443, 806, 865
HinP1I GCGC 3 cut(s) 443, 806, 865
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 5 cut(s) 7, 18, 569, 622, 814
HpaII CCGG 4 cut(s) 47, 135, 576, 582
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 2 cut(s) 6, 573
Hpy188I TCNGA 5 cut(s) 12, 120, 231, 621, 693
Hpy188III TCNNGA 4 cut(s) 22, 562, 626, 875
Hpy8I GTNNAC 2 cut(s) 6, 573
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 4 cut(s) 66, 164, 283, 819
HpyCH4III ACNGT 3 cut(s) 164, 661, 750
HpyCH4V TGCA 2 cut(s) 313, 476
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 42, 379, 791, 844
HpyF3I CTNAG 5 cut(s) 11, 369, 627, 816, 873
Hsp92I GRCGYC 2 cut(s) 86, 585
Hsp92II CATG 4 cut(s) 449, 480, 715, 782
HspAI GCGC 3 cut(s) 443, 806, 865
Kzo9I GATC 2 cut(s) 115, 393
LmnI GCTCC 5 cut(s) 24, 173, 231, 672, 775
Lsp1109I GCAGC 4 cut(s) 188, 316, 394, 397
MaeI CTAG 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 188, 315
MalI GATC 2 cut(s) 117, 395
MbiI CCGCTC 1 cut(s) 15
MboI GATC 2 cut(s) 115, 393
MboII GAAGA 9 cut(s) 51, 166, 239, 420, 447, 479, 580, 731, 810
MflI RGATCY 2 cut(s) 115, 393
MhlI GDGCHC 3 cut(s) 224, 236, 326
MlyI GAGTC 3 cut(s) 578, 616, 808
MmeI TCCRAC 3 cut(s) 104, 633, 644
Mph1103I ATGCAT 1 cut(s) 478
MseI TTAA 2 cut(s) 357, 833
MspI CCGG 4 cut(s) 47, 135, 576, 582
MspR9I CCNGG 3 cut(s) 47, 65, 112
MvaI CCWGG 2 cut(s) 65, 112
MvnI CGCG 1 cut(s) 806
MwoI GCNNNNNNNGC 5 cut(s) 33, 42, 379, 791, 844
NciI CCSGG 1 cut(s) 47
NdeII GATC 2 cut(s) 115, 393
NlaIII CATG 4 cut(s) 449, 480, 715, 782
NlaIV GGNNCC 6 cut(s) 54, 69, 222, 233, 580, 771
NmuCI GTSAC 1 cut(s) 315
NsbI TGCGCA 1 cut(s) 444
NsiI ATGCAT 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 529
PfeI GAWTC 1 cut(s) 18
PkrI GCNGC 6 cut(s) 130, 133, 178, 331, 384, 387
PleI GAGTC 3 cut(s) 577, 616, 808
PpsI GAGTC 3 cut(s) 577, 616, 808
PpuMI RGGWCCY 1 cut(s) 53
Psp124BI GAGCTC 1 cut(s) 326
Psp5II RGGWCCY 1 cut(s) 53
Psp6I CCWGG 2 cut(s) 63, 110
PspFI CCCAGC 1 cut(s) 670
PspGI CCWGG 2 cut(s) 63, 110
PspN4I GGNNCC 6 cut(s) 54, 69, 222, 233, 580, 771
PspOMI GGGCCC 1 cut(s) 220
PspPI GGNCC 5 cut(s) 53, 220, 221, 845, 882
PspPPI RGGWCCY 1 cut(s) 53
PstI CTGCAG 1 cut(s) 315
PsuI RGATCY 2 cut(s) 115, 393
RsaI GTAC 1 cut(s) 309
RsaNI GTAC 1 cut(s) 308
SacI GAGCTC 1 cut(s) 326
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 357, 833
SatI GCNGC 6 cut(s) 129, 132, 177, 330, 383, 386
Sau3AI GATC 2 cut(s) 115, 393
Sau96I GGNCC 5 cut(s) 53, 220, 221, 845, 882
ScaI AGTACT 1 cut(s) 309
SchI GAGTC 3 cut(s) 578, 616, 808
ScrFI CCNGG 3 cut(s) 47, 65, 112
SduI GDGCHC 3 cut(s) 224, 236, 326
SfcI CTRYAG 1 cut(s) 311
SgrAI CRCCGGYG 1 cut(s) 581
SinI GGWCC 2 cut(s) 53, 882
SmlI CTYRAG 1 cut(s) 697
SmoI CTYRAG 1 cut(s) 697
SsiI CCGC 4 cut(s) 15, 50, 129, 132
SspMI CTAG 1 cut(s) 33
SstI GAGCTC 1 cut(s) 326
StyD4I CCNGG 3 cut(s) 45, 63, 110
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 3 cut(s) 164, 661, 750
TaqI TCGA 1 cut(s) 5
TaqII GACCGA 1 cut(s) 407
TatI WGTACW 1 cut(s) 307
TauI GCSGC 2 cut(s) 131, 134
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 2 cut(s) 357, 833
Tru9I TTAA 2 cut(s) 357, 833
TscAI CASTG 2 cut(s) 169, 766
TseFI GTSAC 1 cut(s) 315
TseI GCWGC 4 cut(s) 176, 329, 382, 385
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 4 cut(s) 173, 441, 728, 873
TspGWI ACGGA 2 cut(s) 54, 231
TspRI CASTG 2 cut(s) 169, 766
VpaK11BI GGWCC 2 cut(s) 53, 882
XmiI GTMKAC 2 cut(s) 5, 572
XspI CTAG 1 cut(s) 33
ZrmI AGTACT 1 cut(s) 309
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.