Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009400

Physical Location & Seq

Genomic Coordinates Forward (+)

26837471 .. 26840463

Gene Structure 2993 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1163400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGACTATGCCTGCAGCAGTTTCAGAAGCTCGTCTCTGCTCCTTCCATGGCGATCCCCGTCTCCTCACCTGCACACTTCCTTCGCTTGCAAGCTCAAATTTATGAAACTGAAGCGTTTGGACATGGCGAAGCATCAAAGAGTGGTCAGGAGTTCTTGTGGGTTCAACGAACATGGCTCCGTCAACGGGTTTCCGGTCATACCCAACAAGCTTTTCATGCAGGAGGCGATTGGAGCTGAATATGGAGAAGGTTTTGAAACTTTTAGACCAGATGGACCTCTTAAGGTTGATGTGGATTTTTTGAACGACAGACTGCAAGAAGGTTTTCTTAAACGAATACGATATGCCATGAAACCAGATGAAGCTTATGGTCTAATATTCTCTTTTGACAATGTCGTGGCTGATACTCGAACTTTGAAATTAAATTCTTGGAAGCAGCTTGCCTCGGAAGAGGGAAAGGAAATTCCCGAAGATGCAGCTTTGCAAAGACGGATGCTTTATGCAGGTGCTGATCATGTGTTGCATAAGCTTTTACTCTGGGACAAAGCAGACGGAGAGCTGGATAGGTTGGCTTTAAAGTTTTCACAATTATACTGTGATAATCTTCTCAGGCTTAGTGAACCCGTGGAGGGGCTCAAAGAGTGGTTAGATGCTGTATCCACAGCTCGTATCCCTTGTGCTGTAGTTTCAAGTCTTGATAGGAGAAACATGACAGAAGCCCTAGAACGAATGGGTCTCAAGAAGTATTTTCAGGCAATTGTGACAGAGGAAGATGGAATGGATTCGATAGCTCATAGGTTTCTTTCAGCAGCTGTGAAGCTGGATCGGAAGCCATCCAAGTGCGTTGTGTTCGAGGATGAACCTAGGGGTATAACCGCTGCTCACAACTGTACAATGATGGCTGTGGCACTGATTGGCGCCCACCCTGCGTATGACCTGGTGCAGGCTGATCTTGCAGTTGCTAGCTTTAACGAACTTTCCGTGATCAATCTTAGAAGACTATTTGCGAACAAGGGTTCTACATTCATGGACTTGCAGAAGCAGGTTATAGAGAAATCTCCACCGAAGAGGAAGCTTACTGTAGATACTATTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

366

Amino Acids

41.25

Weight (kDa)

8.08

Isoelectric Point (pI)

44.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_2	PF13419	126 - 302	1.4e-18	Haloacid dehalogenase-like hydrolase
Hydrolase	PF00702	129 - 296	1.2e-09	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10970 AT3G10970 AT3G10970
fragaria_vesca	FvH4_7g25610
malus_domestica	MD01G1163400.v1.1 MD07G1231400.v1.1
prunus_persica	Prupe.2G261400_v2.0.a1 Prupe.2G261400_v2.0.a1
pyrus_communis	pycom01g17800 pycom07g20920
rosa_chinensis	RchiOBHm_Chr1g0371751
rosa_laevigata	RLG00000026944
rosa_multiflora	Rmu_sc0001755.1_g000053
rosa_roxburghii	Rroxscaffold_4G00285450
rosa_rugosa	Rorug01G0366000 Rorug01G0366100 Rorug01G0366200
rosa_samantha	Rh1AG376100 Rh1BG339500 Rh1CG352600 Rh1DG370700
rosa_wichuraiana	Rw1G033090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	78, 494
Acc36I	ACCTGC	3 cut(s)	78, 494, 1033
AccB1I	GGYRCC	1 cut(s)	917
AciI	CCGC	1 cut(s)	876
AclWI	GGATC	2 cut(s)	48, 831
AcsI	RAATTY	3 cut(s)	98, 424, 462
AcuI	CTGAAG	1 cut(s)	131
AcyI	GRCGYC	1 cut(s)	918
AfaI	GTAC	1 cut(s)	892
AfiI	CCNNNNNNNGG	4 cut(s)	186, 629, 630, 943
AflII	CTTAAG	1 cut(s)	281
AgsI	TTSAA	5 cut(s)	166, 257, 304, 418, 690
AjnI	CCWGG	1 cut(s)	936
AjuI	GAANNNNNNNTTGG	2 cut(s)	197, 229
Alw26I	GTCTC	3 cut(s)	39, 66, 740
AlwI	GGATC	2 cut(s)	48, 831
AlwNI	CAGNNNCTG	2 cut(s)	509, 812
ApeKI	GCWGC	5 cut(s)	15, 436, 476, 809, 878
ApoI	RAATTY	3 cut(s)	98, 424, 462
Asp700I	GAANNNNTTC	2 cut(s)	324, 781
AspA2I	CCTAGG	1 cut(s)	863
AspLEI	GCGC	1 cut(s)	920
AspS9I	GGNCC	1 cut(s)	275
AsuHPI	GGTGA	1 cut(s)	59
AsuNHI	GCTAGC	1 cut(s)	962
AvaII	GGWCC	1 cut(s)	275
AvrII	CCTAGG	1 cut(s)	863
BanI	GGYRCC	1 cut(s)	917
BanII	GRGCYC	1 cut(s)	636
BbsI	GAAGAC	1 cut(s)	1003
BbvI	GCAGC	5 cut(s)	27, 448, 488, 821, 865
BccI	CCATC	4 cut(s)	266, 767, 841, 892
BciT130I	CCWGG	1 cut(s)	938
BciVI	GTATCC	2 cut(s)	667, 680
BclI	TGATCA	2 cut(s)	511, 984
BcoDI	GTCTC	3 cut(s)	39, 66, 740
BfaI	CTAG	3 cut(s)	722, 864, 963
BfmI	CTRYAG	3 cut(s)	13, 681, 1080
BfoI	RGCGCY	1 cut(s)	921
BfrI	CTTAAG	1 cut(s)	281
BfuAI	ACCTGC	3 cut(s)	78, 494, 1033
BfuI	GTATCC	2 cut(s)	667, 680
BisI	GCNGC	5 cut(s)	16, 437, 477, 810, 879
BlnI	CCTAGG	1 cut(s)	863
BlsI	GCNGC	5 cut(s)	17, 438, 478, 811, 880
Bme1390I	CCNGG	1 cut(s)	938
Bme18I	GGWCC	1 cut(s)	275
BmgT120I	GGNCC	1 cut(s)	275
BmiI	GGNNCC	2 cut(s)	178, 919
BmrFI	CCNGG	1 cut(s)	938
BmsI	GCATC	4 cut(s)	142, 463, 483, 640
BmtI	GCTAGC	1 cut(s)	966
BpiI	GAAGAC	1 cut(s)	1003
BpuEI	CTTGAG	1 cut(s)	722
BsaHI	GRCGYC	1 cut(s)	918
BsaI	GGTCTC	1 cut(s)	740
BsaJI	CCNNGG	4 cut(s)	47, 444, 624, 863
BsaWI	WCCGGW	1 cut(s)	193
BsaXI	ACNNNNNCTCC	2 cut(s)	142, 172
Bsc4I	CCNNNNNNNGG	4 cut(s)	186, 629, 630, 943
BseBI	CCWGG	1 cut(s)	938
BseDI	CCNNGG	4 cut(s)	47, 444, 624, 863
BseGI	GGATG	3 cut(s)	498, 833, 862
BseLI	CCNNNNNNNGG	4 cut(s)	186, 629, 630, 943
BseMII	CTCAG	1 cut(s)	622
BseRI	GAGGAG	1 cut(s)	54
BseXI	GCAGC	5 cut(s)	27, 448, 488, 821, 865
BsgI	GTGCAG	2 cut(s)	56, 962
BshNI	GGYRCC	1 cut(s)	917
BsiSI	CCGG	1 cut(s)	194
BslFI	GGGAC	1 cut(s)	554
BslI	CCNNNNNNNGG	4 cut(s)	186, 629, 630, 943
BsmAI	GTCTC	3 cut(s)	39, 66, 740
BsmBI	CGTCTC	2 cut(s)	39, 66
BsmFI	GGGAC	1 cut(s)	554
Bso31I	GGTCTC	1 cut(s)	740
Bsp1286I	GDGCHC	1 cut(s)	636
Bsp1407I	TGTACA	1 cut(s)	890
Bsp143I	GATC	5 cut(s)	53, 511, 823, 949, 984
Bsp19I	CCATGG	1 cut(s)	47
BspACI	CCGC	1 cut(s)	876
BspCNI	CTCAG	1 cut(s)	621
BspLI	GGNNCC	2 cut(s)	178, 919
BspMAI	CTGCAG	1 cut(s)	17
BspMI	ACCTGC	3 cut(s)	78, 494, 1033
BspOI	GCTAGC	1 cut(s)	966
BspPI	GGATC	2 cut(s)	48, 831
BspT107I	GGYRCC	1 cut(s)	917
BspTI	CTTAAG	1 cut(s)	281
BspTNI	GGTCTC	1 cut(s)	740
BsrGI	TGTACA	1 cut(s)	890
BssECI	CCNNGG	4 cut(s)	47, 444, 624, 863
BssMI	GATC	5 cut(s)	53, 511, 823, 949, 984
BssNI	GRCGYC	1 cut(s)	918
BssT1I	CCWWGG	2 cut(s)	47, 863
Bst2UI	CCWGG	1 cut(s)	938
Bst4CI	ACNGT	3 cut(s)	596, 890, 1081
Bst6I	CTCTTC	2 cut(s)	444, 1061
BstACI	GRCGYC	1 cut(s)	918
BstAFI	CTTAAG	1 cut(s)	281
BstAUI	TGTACA	1 cut(s)	890
BstC8I	GCNNGC	6 cut(s)	13, 88, 92, 441, 945, 964
BstDEI	CTNAG	3 cut(s)	608, 614, 992
BstDSI	CCRYGG	2 cut(s)	47, 624
BstENI	CCTNNNNNAGG	1 cut(s)	941
BstF5I	GGATG	3 cut(s)	498, 833, 862
BstH2I	RGCGCY	1 cut(s)	921
BstHHI	GCGC	1 cut(s)	920
BstKTI	GATC	5 cut(s)	56, 514, 826, 952, 987
BstMAI	GTCTC	3 cut(s)	39, 66, 740
BstMBI	GATC	5 cut(s)	53, 511, 823, 949, 984
BstMWI	GCNNNNNNNGC	4 cut(s)	217, 233, 926, 953
BstNI	CCWGG	1 cut(s)	938
BstSCI	CCNGG	1 cut(s)	936
BstSFI	CTRYAG	3 cut(s)	13, 681, 1080
BstV1I	GCAGC	5 cut(s)	27, 448, 488, 821, 865
BstV2I	GAAGAC	1 cut(s)	1003
BsuI	GTATCC	2 cut(s)	667, 680
BtgI	CCRYGG	2 cut(s)	47, 624
BtsCI	GGATG	3 cut(s)	498, 833, 862
BtsIMutI	CAGTG	1 cut(s)	908
BveI	ACCTGC	3 cut(s)	78, 494, 1033
Cac8I	GCNNGC	6 cut(s)	13, 88, 92, 441, 945, 964
CaiI	CAGNNNCTG	2 cut(s)	509, 812
CfoI	GCGC	1 cut(s)	920
Cfr13I	GGNCC	1 cut(s)	275
CsiI	ACCWGGT	1 cut(s)	936
Csp6I	GTAC	1 cut(s)	891
CviAII	CATG	8 cut(s)	48, 124, 173, 217, 349, 515, 709, 1027
CviQI	GTAC	1 cut(s)	891
DdeI	CTNAG	3 cut(s)	608, 614, 992
DinI	GGCGCC	1 cut(s)	919
DpnI	GATC	5 cut(s)	55, 513, 825, 951, 986
DpnII	GATC	5 cut(s)	53, 511, 823, 949, 984
DraI	TTTAAA	1 cut(s)	576
Eam1104I	CTCTTC	2 cut(s)	444, 1061
EarI	CTCTTC	2 cut(s)	444, 1061
Eco130I	CCWWGG	2 cut(s)	47, 863
Eco24I	GRGCYC	1 cut(s)	636
Eco31I	GGTCTC	1 cut(s)	740
Eco47I	GGWCC	1 cut(s)	275
Eco57I	CTGAAG	1 cut(s)	131
EcoNI	CCTNNNNNAGG	1 cut(s)	941
EcoRII	CCWGG	1 cut(s)	936
EcoT14I	CCWWGG	2 cut(s)	47, 863
EcoT38I	GRGCYC	1 cut(s)	636
EgeI	GGCGCC	1 cut(s)	919
EheI	GGCGCC	1 cut(s)	919
ErhI	CCWWGG	2 cut(s)	47, 863
Esp3I	CGTCTC	2 cut(s)	39, 66
FaeI	CATG	8 cut(s)	51, 127, 176, 220, 352, 518, 712, 1030
FalI	AAGNNNNNCTT	2 cut(s)	312, 344
FaqI	GGGAC	1 cut(s)	554
FatI	CATG	8 cut(s)	47, 123, 172, 216, 348, 514, 708, 1026
FbaI	TGATCA	2 cut(s)	511, 984
Fnu4HI	GCNGC	5 cut(s)	16, 437, 477, 810, 879
FokI	GGATG	3 cut(s)	505, 820, 869
FriOI	GRGCYC	1 cut(s)	636
Fsp4HI	GCNGC	5 cut(s)	16, 437, 477, 810, 879
FspBI	CTAG	3 cut(s)	722, 864, 963
GlaI	GCGC	1 cut(s)	919
GluI	GCNGC	5 cut(s)	16, 437, 477, 810, 879
HaeII	RGCGCY	1 cut(s)	921
HapII	CCGG	1 cut(s)	194
HhaI	GCGC	1 cut(s)	920
Hin1I	GRCGYC	1 cut(s)	918
Hin1II	CATG	8 cut(s)	51, 127, 176, 220, 352, 518, 712, 1030
Hin6I	GCGC	1 cut(s)	918
HinP1I	GCGC	1 cut(s)	918
HincII	GTYRAC	1 cut(s)	184
HindII	GTYRAC	1 cut(s)	184
HindIII	AAGCTT	4 cut(s)	209, 363, 527, 1073
HinfI	GANTC	1 cut(s)	782
HpaII	CCGG	1 cut(s)	194
HphI	GGTGA	1 cut(s)	59
Hpy166II	GTNNAC	2 cut(s)	184, 620
Hpy188I	TCNGA	4 cut(s)	26, 448, 828, 1097
Hpy188III	TCNNGA	4 cut(s)	148, 467, 695, 739
Hpy8I	GTNNAC	2 cut(s)	184, 620
HpyAV	CCTTC	4 cut(s)	53, 91, 242, 314
HpyCH4III	ACNGT	3 cut(s)	596, 890, 1081
HpyF10VI	GCNNNNNNNGC	4 cut(s)	217, 233, 926, 953
HpyF3I	CTNAG	3 cut(s)	608, 614, 992
Hsp92I	GRCGYC	1 cut(s)	918
Hsp92II	CATG	8 cut(s)	51, 127, 176, 220, 352, 518, 712, 1030
HspAI	GCGC	1 cut(s)	918
KasI	GGCGCC	1 cut(s)	917
Ksp22I	TGATCA	2 cut(s)	511, 984
Kzo9I	GATC	5 cut(s)	53, 511, 823, 949, 984
LmnI	GCTCC	3 cut(s)	45, 182, 233
Lsp1109I	GCAGC	5 cut(s)	27, 448, 488, 821, 865
LweI	GCATC	4 cut(s)	142, 463, 483, 640
MabI	ACCWGGT	1 cut(s)	936
MaeI	CTAG	3 cut(s)	722, 864, 963
MaeIII	GTNAC	1 cut(s)	760
MalI	GATC	5 cut(s)	55, 513, 825, 951, 986
MboI	GATC	5 cut(s)	53, 511, 823, 949, 984
MboII	GAAGA	6 cut(s)	461, 482, 596, 782, 1008, 1078
MfeI	CAATTG	1 cut(s)	756
MhlI	GDGCHC	1 cut(s)	636
MluCI	AATT	6 cut(s)	98, 419, 424, 462, 587, 756
Mly113I	GGCGCC	1 cut(s)	918
MnlI	CCTC	9 cut(s)	75, 217, 288, 445, 454, 622, 760, 847, 1062
MroXI	GAANNNNTTC	2 cut(s)	324, 781
MseI	TTAA	5 cut(s)	282, 330, 422, 575, 969
MslI	CAYNNNNRTG	1 cut(s)	838
MspA1I	CMGCKG	2 cut(s)	812, 878
MspCI	CTTAAG	1 cut(s)	281
MspI	CCGG	1 cut(s)	194
MspR9I	CCNGG	1 cut(s)	938
MunI	CAATTG	1 cut(s)	756
MvaI	CCWGG	1 cut(s)	938
MwoI	GCNNNNNNNGC	4 cut(s)	217, 233, 926, 953
NarI	GGCGCC	1 cut(s)	918
NcoI	CCATGG	1 cut(s)	47
NdeII	GATC	5 cut(s)	53, 511, 823, 949, 984
NheI	GCTAGC	1 cut(s)	962
NlaIII	CATG	8 cut(s)	51, 127, 176, 220, 352, 518, 712, 1030
NlaIV	GGNNCC	2 cut(s)	178, 919
NmuCI	GTSAC	1 cut(s)	760
PaqCI	CACCTGC	2 cut(s)	78, 494
PcsI	WCGNNNNNNNCGW	1 cut(s)	978
PdmI	GAANNNNTTC	2 cut(s)	324, 781
PfeI	GAWTC	1 cut(s)	782
PflFI	GACNNNGTC	1 cut(s)	392
PkrI	GCNGC	5 cut(s)	17, 438, 478, 811, 880
PluTI	GGCGCC	1 cut(s)	921
Psp6I	CCWGG	1 cut(s)	936
PspGI	CCWGG	1 cut(s)	936
PspN4I	GGNNCC	2 cut(s)	178, 919
PspPI	GGNCC	1 cut(s)	275
PstI	CTGCAG	1 cut(s)	17
PstNI	CAGNNNCTG	2 cut(s)	509, 812
PsyI	GACNNNGTC	1 cut(s)	392
PvuII	CAGCTG	1 cut(s)	812
RsaI	GTAC	1 cut(s)	892
RsaNI	GTAC	1 cut(s)	891
RseI	CAYNNNNRTG	1 cut(s)	838
SaqAI	TTAA	5 cut(s)	282, 330, 422, 575, 969
SatI	GCNGC	5 cut(s)	16, 437, 477, 810, 879
Sau3AI	GATC	5 cut(s)	53, 511, 823, 949, 984
Sau96I	GGNCC	1 cut(s)	275
ScrFI	CCNGG	1 cut(s)	938
SduI	GDGCHC	1 cut(s)	636
SexAI	ACCWGGT	1 cut(s)	936
SfaNI	GCATC	4 cut(s)	142, 463, 483, 640
SfcI	CTRYAG	3 cut(s)	13, 681, 1080
SfoI	GGCGCC	1 cut(s)	919
SinI	GGWCC	1 cut(s)	275
SmiMI	CAYNNNNRTG	1 cut(s)	838
SmlI	CTYRAG	2 cut(s)	281, 737
SmoI	CTYRAG	2 cut(s)	281, 737
Sse9I	AATT	6 cut(s)	98, 419, 424, 462, 587, 756
SsiI	CCGC	1 cut(s)	876
SspDI	GGCGCC	1 cut(s)	917
SspI	AATATT	1 cut(s)	378
SspMI	CTAG	3 cut(s)	722, 864, 963
StyD4I	CCNGG	1 cut(s)	936
StyI	CCWWGG	2 cut(s)	47, 863
TaaI	ACNGT	3 cut(s)	596, 890, 1081
TaqI	TCGA	3 cut(s)	409, 785, 852
TasI	AATT	6 cut(s)	98, 419, 424, 462, 587, 756
TatI	WGTACW	1 cut(s)	890
TfiI	GAWTC	1 cut(s)	782
Tru1I	TTAA	5 cut(s)	282, 330, 422, 575, 969
Tru9I	TTAA	5 cut(s)	282, 330, 422, 575, 969
TscAI	CASTG	1 cut(s)	915
TseFI	GTSAC	1 cut(s)	760
TseI	GCWGC	5 cut(s)	15, 436, 476, 809, 878
Tsp45I	GTSAC	1 cut(s)	760
TspDTI	ATGAA	6 cut(s)	119, 205, 365, 375, 873, 1015
TspGWI	ACGGA	4 cut(s)	169, 505, 567, 970
TspRI	CASTG	1 cut(s)	915
Tth111I	GACNNNGTC	1 cut(s)	392
Vha464I	CTTAAG	1 cut(s)	281
VpaK11BI	GGWCC	1 cut(s)	275
XagI	CCTNNNNNAGG	1 cut(s)	941
XapI	RAATTY	3 cut(s)	98, 424, 462
XmaJI	CCTAGG	1 cut(s)	863
XmnI	GAANNNNTTC	2 cut(s)	324, 781
XspI	CTAG	3 cut(s)	722, 864, 963

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.