Prupe.2G261400_v2.0.a1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0009400
Physical Location & Seq
Forward (+)
27054530 .. 27058098
3569 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G261400.1

Sequence Viewer

Length: 1098 bp
ATGGACTGTGCTTGCAGCAATTTCAGAAGCTCGTCTTTGCTTCTTCTCGGGCGATCCCCATCTCTTCATCCACACTATTCGTTCCCTTCCAAGCTCAGATTCTTGACACTGAAGCGTTTGAACCTGGCTAAGCATGCCCGGGTGGTAAGGAGTGCTTGTGGGTTCAACGAAAACGGCTCTGTTAATGGGTTTTCCGTCACACCCAACAAGCTTTTCATGCAAGAGGCAATTGGAGCTGAATATGGAGAAGGTTTTGAGACTTTTAGACCAGATGGGCCTCTCAAGGTTGATGTGGATTTTTTGAACGACAGACTCCAAGAAGGTTTTCTTCAACGTATACGATATGCCATGAAACCTGATGAAGCGTATGGTCTTATTTTCTCTTTTGACAATGTTGTGGCTGATACTCGAACATTGAAATTAAATTCTTGGAAGCAACTTGCCTCAGAAGAGGGAAAGGAAATTCCTGAAGATGCGGATGTGCAAGGACGAATGCTTTATGCAGGTGCTGATCATGTGTTGCATAAGCTTTTACTCTGGGATGAAGCAGAAGGAGAACTAGATAGATTGGCGTTAAGGTTTTCACAGCTATATTATGATAATCTTCTCAGGCTTAGTAAACCTGTGGAGGGGCTCAAGGAATGGCTAGATGCTGTATCCACAGCTCGTATCCCTTGTGCTGTAGTTTCAAGTCTTGATCGGATAAACATGACAGAAGCCCTAGAACGAATGGAACTCAAGAAGTATTTCCAGGCAATTGTGACAGAGGAAGATGGAATGGATTCAATAGCTCATAGGTTTCTTTCTGCAGCTATGAAGCTGGACCGAAAACCATCGAAGTGTGTAGTGTTTGAGGATGAGCCTAGAGGTATAGCTGCTGCCCACAACTGTACAATGATGGCGGTGGCATTGATCGGTGCCCACCCTGCGTATGACCTGGTTCAGGCAGATCTTGCAGTTGCTAGTTTTAATGAACTTTCAGTGATCAATCTCCGAAGACTATTTGCAAACAAGGGTTCTACATTCATGGACTTACAAAAGCAGGTTATAGAAAAATCTCCACCAAAGAGGAAGCTTACAATAGATACTATTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

366

Amino Acids

41.1

Weight (kDa)

6.28

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 494
Acc36I ACCTGC 2 cut(s) 494, 1033
AccB1I GGYRCC 1 cut(s) 917
AccI GTMKAC 1 cut(s) 337
AciI CCGC 2 cut(s) 476, 902
AclWI GGATC 1 cut(s) 48
AcsI RAATTY 2 cut(s) 424, 462
AcuI CTGAAG 2 cut(s) 131, 489
AfaI GTAC 1 cut(s) 892
AfiI CCNNNNNNNGG 2 cut(s) 629, 943
AgsI TTSAA 7 cut(s) 121, 166, 304, 332, 418, 690, 786
AjnI CCWGG 3 cut(s) 123, 750, 936
AjuI GAANNNNNNNTTGG 6 cut(s) 83, 115, 197, 229, 309, 341
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 48
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 2 cut(s) 47, 138
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 4 cut(s) 15, 809, 875, 878
ApoI RAATTY 2 cut(s) 424, 462
Asp700I GAANNNNTTC 3 cut(s) 324, 746, 781
AspS9I GGNCC 2 cut(s) 275, 823
AsuC2I CCSGG 2 cut(s) 139, 140
AvaI CYCGRG 2 cut(s) 47, 138
AvaII GGWCC 1 cut(s) 823
BaeGI GKGCMC 1 cut(s) 922
BanI GGYRCC 1 cut(s) 917
BanII GRGCYC 1 cut(s) 636
BbsI GAAGAC 1 cut(s) 1003
BbvI GCAGC 4 cut(s) 27, 821, 862, 865
BccI CCATC 5 cut(s) 67, 266, 767, 841, 892
BceAI ACGGC 1 cut(s) 190
BciT130I CCWGG 3 cut(s) 125, 752, 938
BciVI GTATCC 2 cut(s) 667, 680
BclI TGATCA 2 cut(s) 511, 984
BcnI CCSGG 2 cut(s) 139, 140
BcoDI GTCTC 1 cut(s) 251
BfaI CTAG 5 cut(s) 560, 647, 722, 864, 963
BfmI CTRYAG 2 cut(s) 681, 807
BfuAI ACCTGC 2 cut(s) 494, 1033
BfuI GTATCC 2 cut(s) 667, 680
BglII AGATCT 1 cut(s) 949
BisI GCNGC 4 cut(s) 16, 810, 876, 879
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 4 cut(s) 17, 811, 877, 880
Bme1390I CCNGG 5 cut(s) 125, 139, 140, 752, 938
Bme18I GGWCC 1 cut(s) 823
BmeT110I CYCGRG 2 cut(s) 47, 138
BmgT120I GGNCC 2 cut(s) 275, 823
BmiI GGNNCC 1 cut(s) 919
BmrFI CCNGG 5 cut(s) 125, 139, 140, 752, 938
BmsI GCATC 2 cut(s) 463, 640
BpiI GAAGAC 1 cut(s) 1003
Bpu1102I GCTNAGC 1 cut(s) 129
BpuEI CTTGAG 3 cut(s) 266, 620, 722
BpuMI CCSGG 2 cut(s) 139, 140
BsaBI GATNNNNATC 1 cut(s) 58
BsaJI CCNNGG 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 943
Bse8I GATNNNNATC 1 cut(s) 58
BseBI CCWGG 3 cut(s) 125, 752, 938
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 4 cut(s) 67, 484, 547, 862
BseJI GATNNNNATC 1 cut(s) 58
BseLI CCNNNNNNNGG 2 cut(s) 629, 943
BseMII CTCAG 3 cut(s) 109, 459, 622
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 4 cut(s) 27, 821, 862, 865
BshFI GGCC 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 917
BsiHKCI CYCGRG 2 cut(s) 47, 138
BsiSI CCGG 1 cut(s) 139
BslI CCNNNNNNNGG 2 cut(s) 629, 943
BsmAI GTCTC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 1 cut(s) 277
BsoBI CYCGRG 2 cut(s) 47, 138
Bsp1286I GDGCHC 2 cut(s) 636, 922
Bsp1407I TGTACA 1 cut(s) 890
Bsp143I GATC 6 cut(s) 53, 511, 697, 912, 949, 984
Bsp1720I GCTNAGC 1 cut(s) 129
BspACI CCGC 2 cut(s) 476, 902
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 3 cut(s) 108, 458, 621
BspLI GGNNCC 1 cut(s) 919
BspMAI CTGCAG 1 cut(s) 811
BspMI ACCTGC 2 cut(s) 494, 1033
BspPI GGATC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 917
BsrGI TGTACA 1 cut(s) 890
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 6 cut(s) 53, 511, 697, 912, 949, 984
BssNAI GTATAC 1 cut(s) 338
Bst1107I GTATAC 1 cut(s) 338
Bst2UI CCWGG 3 cut(s) 125, 752, 938
Bst4CI ACNGT 2 cut(s) 8, 890
Bst6I CTCTTC 2 cut(s) 69, 444
BstAPI GCANNNNNTGC 1 cut(s) 953
BstAUI TGTACA 1 cut(s) 890
BstC8I GCNNGC 2 cut(s) 13, 135
BstDEI CTNAG 5 cut(s) 95, 129, 445, 608, 614
BstENI CCTNNNNNAGG 1 cut(s) 941
BstF5I GGATG 4 cut(s) 67, 484, 547, 862
BstKTI GATC 6 cut(s) 56, 514, 700, 915, 952, 987
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 6 cut(s) 53, 511, 697, 912, 949, 984
BstMWI GCNNNNNNNGC 5 cut(s) 134, 217, 233, 926, 953
BstNI CCWGG 3 cut(s) 125, 752, 938
BstNSI RCATGY 1 cut(s) 137
BstSCI CCNGG 5 cut(s) 123, 137, 138, 750, 936
BstSFI CTRYAG 2 cut(s) 681, 807
BstSLI GKGCMC 1 cut(s) 922
BstV1I GCAGC 4 cut(s) 27, 821, 862, 865
BstV2I GAAGAC 1 cut(s) 1003
BstX2I RGATCY 1 cut(s) 949
BstYI RGATCY 1 cut(s) 949
BstZ17I GTATAC 1 cut(s) 338
BsuI GTATCC 2 cut(s) 667, 680
BsuRI GGCC 1 cut(s) 277
BtsCI GGATG 4 cut(s) 67, 484, 547, 862
BtsIMutI CAGTG 2 cut(s) 107, 987
BveI ACCTGC 2 cut(s) 494, 1033
Cac8I GCNNGC 2 cut(s) 13, 135
CaiI CAGNNNCTG 1 cut(s) 509
Cfr13I GGNCC 2 cut(s) 275, 823
Cfr9I CCCGGG 1 cut(s) 138
CsiI ACCWGGT 1 cut(s) 936
Csp6I GTAC 1 cut(s) 891
CviAII CATG 6 cut(s) 134, 217, 349, 515, 709, 1027
CviQI GTAC 1 cut(s) 891
DdeI CTNAG 5 cut(s) 95, 129, 445, 608, 614
DpnI GATC 6 cut(s) 55, 513, 699, 914, 951, 986
DpnII GATC 6 cut(s) 53, 511, 697, 912, 949, 984
Eam1104I CTCTTC 2 cut(s) 69, 444
EarI CTCTTC 2 cut(s) 69, 444
Eco24I GRGCYC 1 cut(s) 636
Eco47I GGWCC 1 cut(s) 823
Eco57I CTGAAG 2 cut(s) 131, 489
Eco88I CYCGRG 2 cut(s) 47, 138
EcoNI CCTNNNNNAGG 1 cut(s) 941
EcoRII CCWGG 3 cut(s) 123, 750, 936
EcoT38I GRGCYC 1 cut(s) 636
FaeI CATG 6 cut(s) 137, 220, 352, 518, 712, 1030
FalI AAGNNNNNCTT 6 cut(s) 19, 51, 139, 171, 312, 344
FatI CATG 6 cut(s) 133, 216, 348, 514, 708, 1026
FbaI TGATCA 2 cut(s) 511, 984
FblI GTMKAC 1 cut(s) 337
Fnu4HI GCNGC 4 cut(s) 16, 810, 876, 879
FokI GGATG 4 cut(s) 54, 491, 554, 869
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 4 cut(s) 16, 810, 876, 879
FspBI CTAG 5 cut(s) 560, 647, 722, 864, 963
GluI GCNGC 4 cut(s) 16, 810, 876, 879
HaeIII GGCC 1 cut(s) 277
HapII CCGG 1 cut(s) 139
Hin1II CATG 6 cut(s) 137, 220, 352, 518, 712, 1030
HindIII AAGCTT 3 cut(s) 209, 527, 1073
HinfI GANTC 3 cut(s) 99, 312, 782
HpaII CCGG 1 cut(s) 139
Hpy166II GTNNAC 2 cut(s) 338, 620
Hpy188I TCNGA 6 cut(s) 26, 98, 448, 702, 995, 1097
Hpy188III TCNNGA 4 cut(s) 103, 467, 695, 739
Hpy8I GTNNAC 2 cut(s) 338, 620
HpyAV CCTTC 4 cut(s) 96, 242, 314, 545
HpyCH4III ACNGT 2 cut(s) 8, 890
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 8 cut(s) 15, 220, 484, 503, 523, 809, 956, 1007
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 217, 233, 926, 953
HpyF3I CTNAG 5 cut(s) 95, 129, 445, 608, 614
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 6 cut(s) 137, 220, 352, 518, 712, 1030
Ksp22I TGATCA 2 cut(s) 511, 984
Kzo9I GATC 6 cut(s) 53, 511, 697, 912, 949, 984
LmnI GCTCC 1 cut(s) 233
Lsp1109I GCAGC 4 cut(s) 27, 821, 862, 865
LweI GCATC 2 cut(s) 463, 640
MabI ACCWGGT 1 cut(s) 936
MaeI CTAG 5 cut(s) 560, 647, 722, 864, 963
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 2 cut(s) 196, 760
MalI GATC 6 cut(s) 55, 513, 699, 914, 951, 986
MboI GATC 6 cut(s) 53, 511, 697, 912, 949, 984
MboII GAAGA 8 cut(s) 35, 56, 320, 461, 482, 596, 782, 1008
MfeI CAATTG 2 cut(s) 228, 756
MflI RGATCY 1 cut(s) 949
MhlI GDGCHC 2 cut(s) 636, 922
MluCI AATT 6 cut(s) 19, 228, 419, 424, 462, 756
MlyI GAGTC 1 cut(s) 306
MnlI CCTC 9 cut(s) 217, 288, 445, 454, 622, 760, 847, 860, 1062
MroXI GAANNNNTTC 3 cut(s) 324, 746, 781
MseI TTAA 4 cut(s) 183, 422, 575, 969
MslI CAYNNNNRTG 1 cut(s) 838
MspI CCGG 1 cut(s) 139
MspR9I CCNGG 5 cut(s) 125, 139, 140, 752, 938
MunI CAATTG 2 cut(s) 228, 756
Mva1269I GAATGC 1 cut(s) 498
MvaI CCWGG 3 cut(s) 125, 752, 938
MwoI GCNNNNNNNGC 5 cut(s) 134, 217, 233, 926, 953
NciI CCSGG 2 cut(s) 139, 140
NdeII GATC 6 cut(s) 53, 511, 697, 912, 949, 984
NlaIII CATG 6 cut(s) 137, 220, 352, 518, 712, 1030
NlaIV GGNNCC 1 cut(s) 919
NmuCI GTSAC 2 cut(s) 196, 760
NspI RCATGY 1 cut(s) 137
PaeI GCATGC 1 cut(s) 137
PaqCI CACCTGC 1 cut(s) 494
PctI GAATGC 1 cut(s) 498
PdmI GAANNNNTTC 3 cut(s) 324, 746, 781
PfeI GAWTC 2 cut(s) 99, 782
PkrI GCNGC 4 cut(s) 17, 811, 877, 880
PleI GAGTC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 306
Psp6I CCWGG 3 cut(s) 123, 750, 936
PspGI CCWGG 3 cut(s) 123, 750, 936
PspN4I GGNNCC 1 cut(s) 919
PspPI GGNCC 2 cut(s) 275, 823
PstI CTGCAG 1 cut(s) 811
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 1 cut(s) 949
RsaI GTAC 1 cut(s) 892
RsaNI GTAC 1 cut(s) 891
RseI CAYNNNNRTG 1 cut(s) 838
SaqAI TTAA 4 cut(s) 183, 422, 575, 969
SatI GCNGC 4 cut(s) 16, 810, 876, 879
Sau3AI GATC 6 cut(s) 53, 511, 697, 912, 949, 984
Sau96I GGNCC 2 cut(s) 275, 823
SchI GAGTC 1 cut(s) 306
ScrFI CCNGG 5 cut(s) 125, 139, 140, 752, 938
SduI GDGCHC 2 cut(s) 636, 922
SexAI ACCWGGT 1 cut(s) 936
SfaNI GCATC 2 cut(s) 463, 640
SfcI CTRYAG 2 cut(s) 681, 807
SinI GGWCC 1 cut(s) 823
SmaI CCCGGG 1 cut(s) 140
SmiMI CAYNNNNRTG 1 cut(s) 838
SmlI CTYRAG 3 cut(s) 281, 635, 737
SmoI CTYRAG 3 cut(s) 281, 635, 737
SphI GCATGC 1 cut(s) 137
Sse9I AATT 6 cut(s) 19, 228, 419, 424, 462, 756
SsiI CCGC 2 cut(s) 476, 902
SspMI CTAG 5 cut(s) 560, 647, 722, 864, 963
StyD4I CCNGG 5 cut(s) 123, 137, 138, 750, 936
TaaI ACNGT 2 cut(s) 8, 890
TaiI ACGT 1 cut(s) 337
TaqI TCGA 2 cut(s) 409, 836
TaqII GACCGA 1 cut(s) 840
TasI AATT 6 cut(s) 19, 228, 419, 424, 462, 756
TatI WGTACW 1 cut(s) 890
TfiI GAWTC 2 cut(s) 99, 782
Tru1I TTAA 4 cut(s) 183, 422, 575, 969
Tru9I TTAA 4 cut(s) 183, 422, 575, 969
TscAI CASTG 2 cut(s) 114, 987
TseFI GTSAC 2 cut(s) 196, 760
TseI GCWGC 4 cut(s) 15, 809, 875, 878
Tsp45I GTSAC 2 cut(s) 196, 760
TspDTI ATGAA 8 cut(s) 56, 205, 365, 375, 558, 830, 987, 1015
TspGWI ACGGA 1 cut(s) 184
TspMI CCCGGG 1 cut(s) 138
TspRI CASTG 2 cut(s) 114, 987
VpaK11BI GGWCC 1 cut(s) 823
XagI CCTNNNNNAGG 1 cut(s) 941
XapI RAATTY 2 cut(s) 424, 462
XceI RCATGY 1 cut(s) 137
XmaI CCCGGG 1 cut(s) 138
XmiI GTMKAC 1 cut(s) 337
XmnI GAANNNNTTC 3 cut(s) 324, 746, 781
XspI CTAG 5 cut(s) 560, 647, 722, 864, 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.