Rw1G033090

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009400
Physical Location & Seq
Forward (+)
61262666 .. 61265780
3115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033090.1

Sequence Viewer

Length: 1212 bp
ATGGACTGCGCTTGCAGTAATTTCAGAAGCTCTTCTCTGCTTCATCAATGGCGATCTCCCTCTCCGCTTCCTCACCGTCTCTTATCGTCAAAGCTCGCCATATTGAGAGTAAAGCGTTTGAACCTGGCTAAGCATGGCCGGCTGGTCACCAGAGCTTGTGGGTCTGACGAAAATAGCTCTGTTAATGGGTTTAACATCACACCCAACAAGCTTTTCATGCAAGAGGCCATTGGAGCTGAATATGGAGAAGGGTTTGAGACTTTTAGACCGGATGGTCCTCTTAAGATTGATGTGGATTTTTTGAACGATAGATTGCAAGAAGGTTTTCTTAAACGGATAAGATATGCCATGAAGCCGGATGAAGCTTATGGGCTTATTTTCTCTTTCGACAATGTTGTGGCTGATACTCGAGCTTTGAAATTAAATGCTTGGAAGCAGCTTGCTGCAGAAGAGGGAAAGGAAATTCCAAAAGAGGCTGATGTGCAAGGACAGATGCTTTATGCAGGTGCTGATCATGTATTGCATAAGCTTTTACTCTGGGATAAAGCAGAGACAGAACTGACTAGGTTGGCGTTAAGGTTTTCGCAACTATTTTATGATAATCTTCTCAGGCTTAGTAAACCCATGGAGGGGCTCAAAGAATGGCTGGAGGCTGTATCTACAGCACGCATCCCTTGTGCTGTTGTTTCAAGTCTTGATCGCAGAAGCATGGTCAAAGCCCTAGAACAAATGGGACTCGAGAAGTATTTTCAGGCAATTGTGACCGAGGAAGATGGCATGGATTCTATAGCTCATAGGTTTCTGTCTGCAGCTGTGAAGCTGGATCGGAAGCCATCCAAGTGTGTAGTGTTTGAGGATGAGCCTAGAGGTATAACTGCCGCTCACAACTGTACGATGATGGCTGTAGGATTGATCGGCGCCCACCCTGCGTATGATCTGGTGCAGGCTGATCTTGCAGTCGCTAGTTTCAATGAACTCCGTAATCAATCTCCGAAGACTATTTGCAAACAAGGGTTCTACATTCATGGACCTCCAAAAACAGATCATAGAGAAATCTCCACCGAAGAGGAAGCTTACAATCGATACTATTTTCTGATTCATTTTTATTCCCCTAATCTAGGCCATCTAGCAGCTTATATATATATGTATATTTTTTTTTTCCTTTTCGGTTCTTGGTTTGAACTTCATCTCGTAATTAGCTTTTGTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

403

Amino Acids

45.84

Weight (kDa)

6.68

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_2 PF13419 126 - 301 2.4e-18 Haloacid dehalogenase-like hydrolase
Hydrolase PF00702 129 - 296 9e-11 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 494
AasI GACNNNNNNGTC 1 cut(s) 273
Acc36I ACCTGC 1 cut(s) 494
AccB1I GGYRCC 1 cut(s) 917
AccBSI CCGCTC 1 cut(s) 881
AciI CCGC 2 cut(s) 65, 879
AclWI GGATC 1 cut(s) 831
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 462
AcyI GRCGYC 1 cut(s) 918
AfaI GTAC 1 cut(s) 892
AfiI CCNNNNNNNGG 3 cut(s) 629, 630, 1118
AflII CTTAAG 1 cut(s) 281
AgsI TTSAA 6 cut(s) 121, 304, 418, 690, 970, 1181
AjnI CCWGG 1 cut(s) 123
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
Alw26I GTCTC 3 cut(s) 83, 251, 545
AlwI GGATC 1 cut(s) 831
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 2 cut(s) 408, 737
AoxI GGCC 3 cut(s) 136, 225, 1120
ApeKI GCWGC 4 cut(s) 436, 443, 809, 1130
ApoI RAATTY 1 cut(s) 462
Asp700I GAANNNNTTC 2 cut(s) 31, 324
AspLEI GCGC 2 cut(s) 11, 920
AspS9I GGNCC 2 cut(s) 275, 1028
AsuHPI GGTGA 2 cut(s) 65, 139
AvaI CYCGRG 2 cut(s) 408, 737
AvaII GGWCC 2 cut(s) 275, 1028
BanI GGYRCC 1 cut(s) 917
BanII GRGCYC 1 cut(s) 636
BbsI GAAGAC 1 cut(s) 1001
BbvI GCAGC 4 cut(s) 430, 448, 821, 1142
BccI CCATC 5 cut(s) 266, 767, 841, 892, 1131
BciT130I CCWGG 1 cut(s) 125
BclI TGATCA 1 cut(s) 511
BcoDI GTCTC 3 cut(s) 83, 251, 545
BfaI CTAG 6 cut(s) 564, 722, 864, 963, 1118, 1127
BfmI CTRYAG 5 cut(s) 444, 660, 786, 807, 903
BfoI RGCGCY 1 cut(s) 921
BfrI CTTAAG 1 cut(s) 281
BfuAI ACCTGC 1 cut(s) 494
BisI GCNGC 5 cut(s) 437, 444, 810, 879, 1131
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 5 cut(s) 438, 445, 811, 880, 1132
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 2 cut(s) 275, 1028
BmeT110I CYCGRG 2 cut(s) 408, 737
BmgT120I GGNCC 2 cut(s) 275, 1028
BmiI GGNNCC 1 cut(s) 919
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 483, 678
BpiI GAAGAC 1 cut(s) 1001
BpmI CTGGAG 1 cut(s) 668
Bpu1102I GCTNAGC 1 cut(s) 129
Bsa29I ATCGAT 1 cut(s) 1081
BsaHI GRCGYC 1 cut(s) 918
BsaJI CCNNGG 2 cut(s) 624, 765
BsaWI WCCGGW 1 cut(s) 268
Bsc4I CCNNNNNNNGG 3 cut(s) 629, 630, 1118
Bse118I RCCGGY 1 cut(s) 138
BseBI CCWGG 1 cut(s) 125
BseCI ATCGAT 1 cut(s) 1081
BseDI CCNNGG 2 cut(s) 624, 765
BseGI GGATG 5 cut(s) 277, 364, 669, 833, 862
BseLI CCNNNNNNNGG 3 cut(s) 629, 630, 1118
BseMII CTCAG 1 cut(s) 622
BseXI GCAGC 4 cut(s) 430, 448, 821, 1142
BsgI GTGCAG 1 cut(s) 962
BshFI GGCC 3 cut(s) 138, 227, 1122
BshNI GGYRCC 1 cut(s) 917
BshVI ATCGAT 1 cut(s) 1081
BsiHKCI CYCGRG 2 cut(s) 408, 737
BsiSI CCGG 3 cut(s) 139, 269, 356
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 3 cut(s) 629, 630, 1118
BsmAI GTCTC 3 cut(s) 83, 251, 545
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 747
BsnI GGCC 3 cut(s) 138, 227, 1122
BsoBI CYCGRG 2 cut(s) 408, 737
Bsp1286I GDGCHC 1 cut(s) 636
Bsp143I GATC 8 cut(s) 53, 511, 697, 823, 912, 934, 949, 1042
Bsp1720I GCTNAGC 1 cut(s) 129
Bsp19I CCATGG 1 cut(s) 624
BspACI CCGC 2 cut(s) 65, 879
BspANI GGCC 3 cut(s) 138, 227, 1122
BspCNI CTCAG 1 cut(s) 621
BspDI ATCGAT 1 cut(s) 1081
BspLI GGNNCC 1 cut(s) 919
BspMAI CTGCAG 2 cut(s) 448, 811
BspMI ACCTGC 1 cut(s) 494
BspPI GGATC 1 cut(s) 831
BspQI GCTCTTC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 917
BspTI CTTAAG 1 cut(s) 281
BsrBI CCGCTC 1 cut(s) 881
BsrFI RCCGGY 1 cut(s) 138
BssAI RCCGGY 1 cut(s) 138
BssECI CCNNGG 2 cut(s) 624, 765
BssMI GATC 8 cut(s) 53, 511, 697, 823, 912, 934, 949, 1042
BssNI GRCGYC 1 cut(s) 918
BssT1I CCWWGG 1 cut(s) 624
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 77, 890
Bst6I CTCTTC 3 cut(s) 37, 444, 1059
BstACI GRCGYC 1 cut(s) 918
BstAFI CTTAAG 1 cut(s) 281
BstC8I GCNNGC 6 cut(s) 13, 96, 140, 441, 667, 945
BstDEI CTNAG 3 cut(s) 129, 608, 614
BstDSI CCRYGG 1 cut(s) 624
BstEII GGTNACC 1 cut(s) 145
BstENI CCTNNNNNAGG 1 cut(s) 1116
BstF5I GGATG 5 cut(s) 277, 364, 669, 833, 862
BstH2I RGCGCY 1 cut(s) 921
BstHHI GCGC 2 cut(s) 11, 920
BstKTI GATC 8 cut(s) 56, 514, 700, 826, 915, 937, 952, 1045
BstMAI GTCTC 3 cut(s) 83, 251, 545
BstMBI GATC 8 cut(s) 53, 511, 697, 823, 912, 934, 949, 1042
BstMWI GCNNNNNNNGC 5 cut(s) 139, 217, 233, 926, 953
BstNI CCWGG 1 cut(s) 125
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 5 cut(s) 444, 660, 786, 807, 903
BstV1I GCAGC 4 cut(s) 430, 448, 821, 1142
BstV2I GAAGAC 1 cut(s) 1001
Bsu15I ATCGAT 1 cut(s) 1081
BsuRI GGCC 3 cut(s) 138, 227, 1122
BsuTUI ATCGAT 1 cut(s) 1081
BtgI CCRYGG 1 cut(s) 624
BtsCI GGATG 5 cut(s) 277, 364, 669, 833, 862
BveI ACCTGC 1 cut(s) 494
Cac8I GCNNGC 6 cut(s) 13, 96, 140, 441, 667, 945
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 2 cut(s) 11, 920
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 2 cut(s) 275, 1028
ClaI ATCGAT 1 cut(s) 1081
Csp6I GTAC 1 cut(s) 891
CviAII CATG 8 cut(s) 134, 217, 349, 515, 625, 709, 778, 1025
CviQI GTAC 1 cut(s) 891
DdeI CTNAG 3 cut(s) 129, 608, 614
DinI GGCGCC 1 cut(s) 919
DpnI GATC 8 cut(s) 55, 513, 699, 825, 914, 936, 951, 1044
DpnII GATC 8 cut(s) 53, 511, 697, 823, 912, 934, 949, 1042
DrdI GACNNNNNNGTC 1 cut(s) 273
DseDI GACNNNNNNGTC 1 cut(s) 273
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 3 cut(s) 37, 444, 1059
EarI CTCTTC 3 cut(s) 37, 444, 1059
Eco130I CCWWGG 1 cut(s) 624
Eco24I GRGCYC 1 cut(s) 636
Eco47I GGWCC 2 cut(s) 275, 1028
Eco88I CYCGRG 2 cut(s) 408, 737
Eco91I GGTNACC 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 1116
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 624
EcoT38I GRGCYC 1 cut(s) 636
EgeI GGCGCC 1 cut(s) 919
EheI GGCGCC 1 cut(s) 919
ErhI CCWWGG 1 cut(s) 624
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 8 cut(s) 137, 220, 352, 518, 628, 712, 781, 1028
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 1 cut(s) 747
FatI CATG 8 cut(s) 133, 216, 348, 514, 624, 708, 777, 1024
FbaI TGATCA 1 cut(s) 511
Fnu4HI GCNGC 5 cut(s) 437, 444, 810, 879, 1131
FokI GGATG 5 cut(s) 284, 371, 656, 820, 869
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 5 cut(s) 437, 444, 810, 879, 1131
FspBI CTAG 6 cut(s) 564, 722, 864, 963, 1118, 1127
GlaI GCGC 2 cut(s) 10, 919
GluI GCNGC 5 cut(s) 437, 444, 810, 879, 1131
GsuI CTGGAG 1 cut(s) 668
HaeII RGCGCY 1 cut(s) 921
HaeIII GGCC 3 cut(s) 138, 227, 1122
HapII CCGG 3 cut(s) 139, 269, 356
HhaI GCGC 2 cut(s) 11, 920
Hin1I GRCGYC 1 cut(s) 918
Hin1II CATG 8 cut(s) 137, 220, 352, 518, 628, 712, 781, 1028
Hin6I GCGC 2 cut(s) 9, 918
HinP1I GCGC 2 cut(s) 9, 918
HindIII AAGCTT 4 cut(s) 209, 363, 527, 1071
HinfI GANTC 3 cut(s) 735, 782, 1096
HpaII CCGG 3 cut(s) 139, 269, 356
HphI GGTGA 2 cut(s) 65, 139
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 5 cut(s) 26, 166, 828, 993, 1095
Hpy188III TCNNGA 2 cut(s) 695, 739
Hpy8I GTNNAC 1 cut(s) 620
HpyAV CCTTC 2 cut(s) 242, 314
HpyCH4III ACNGT 2 cut(s) 77, 890
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 217, 233, 926, 953
HpyF3I CTNAG 3 cut(s) 129, 608, 614
Hsp92I GRCGYC 1 cut(s) 918
Hsp92II CATG 8 cut(s) 137, 220, 352, 518, 628, 712, 781, 1028
HspAI GCGC 2 cut(s) 9, 918
KasI GGCGCC 1 cut(s) 917
KroI GCCGGC 1 cut(s) 138
KroNI GCCGGC 1 cut(s) 140
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 8 cut(s) 53, 511, 697, 823, 912, 934, 949, 1042
LguI GCTCTTC 1 cut(s) 37
LmnI GCTCC 1 cut(s) 233
Lsp1109I GCAGC 4 cut(s) 430, 448, 821, 1142
LweI GCATC 2 cut(s) 483, 678
MaeI CTAG 6 cut(s) 564, 722, 864, 963, 1118, 1127
MaeIII GTNAC 2 cut(s) 145, 760
MalI GATC 8 cut(s) 55, 513, 699, 825, 914, 936, 951, 1044
MbiI CCGCTC 1 cut(s) 881
MboI GATC 8 cut(s) 53, 511, 697, 823, 912, 934, 949, 1042
MboII GAAGA 6 cut(s) 24, 461, 596, 782, 1006, 1076
MfeI CAATTG 1 cut(s) 756
MhlI GDGCHC 1 cut(s) 636
MluCI AATT 5 cut(s) 19, 419, 462, 756, 1194
Mly113I GGCGCC 1 cut(s) 918
MlyI GAGTC 1 cut(s) 729
MroNI GCCGGC 1 cut(s) 138
MroXI GAANNNNTTC 2 cut(s) 31, 324
MseI TTAA 6 cut(s) 183, 192, 282, 330, 422, 575
MslI CAYNNNNRTG 1 cut(s) 838
MspA1I CMGCKG 1 cut(s) 812
MspCI CTTAAG 1 cut(s) 281
MspI CCGG 3 cut(s) 139, 269, 356
MspR9I CCNGG 1 cut(s) 125
MunI CAATTG 1 cut(s) 756
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 5 cut(s) 139, 217, 233, 926, 953
NaeI GCCGGC 1 cut(s) 140
NarI GGCGCC 1 cut(s) 918
NcoI CCATGG 1 cut(s) 624
NdeII GATC 8 cut(s) 53, 511, 697, 823, 912, 934, 949, 1042
NgoMIV GCCGGC 1 cut(s) 138
NlaIII CATG 8 cut(s) 137, 220, 352, 518, 628, 712, 781, 1028
NlaIV GGNNCC 1 cut(s) 919
NmuCI GTSAC 2 cut(s) 145, 760
PaeR7I CTCGAG 2 cut(s) 408, 737
PaqCI CACCTGC 1 cut(s) 494
PciSI GCTCTTC 1 cut(s) 37
PdiI GCCGGC 1 cut(s) 140
PdmI GAANNNNTTC 2 cut(s) 31, 324
PfeI GAWTC 2 cut(s) 782, 1096
PkrI GCNGC 5 cut(s) 438, 445, 811, 880, 1132
PleI GAGTC 1 cut(s) 729
PluTI GGCGCC 1 cut(s) 921
PpsI GAGTC 1 cut(s) 729
Psp6I CCWGG 1 cut(s) 123
PspEI GGTNACC 1 cut(s) 145
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 919
PspPI GGNCC 2 cut(s) 275, 1028
PspXI VCTCGAGB 1 cut(s) 408
PstI CTGCAG 2 cut(s) 448, 811
PstNI CAGNNNCTG 1 cut(s) 509
PvuII CAGCTG 1 cut(s) 812
RsaI GTAC 1 cut(s) 892
RsaNI GTAC 1 cut(s) 891
RseI CAYNNNNRTG 1 cut(s) 838
SapI GCTCTTC 1 cut(s) 37
SaqAI TTAA 6 cut(s) 183, 192, 282, 330, 422, 575
SatI GCNGC 5 cut(s) 437, 444, 810, 879, 1131
Sau3AI GATC 8 cut(s) 53, 511, 697, 823, 912, 934, 949, 1042
Sau96I GGNCC 2 cut(s) 275, 1028
SchI GAGTC 1 cut(s) 729
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 636
SfaNI GCATC 2 cut(s) 483, 678
SfcI CTRYAG 5 cut(s) 444, 660, 786, 807, 903
SfoI GGCGCC 1 cut(s) 919
Sfr274I CTCGAG 2 cut(s) 408, 737
SinI GGWCC 2 cut(s) 275, 1028
SlaI CTCGAG 2 cut(s) 408, 737
SmiMI CAYNNNNRTG 1 cut(s) 838
SmlI CTYRAG 3 cut(s) 281, 408, 737
SmoI CTYRAG 3 cut(s) 281, 408, 737
Sse9I AATT 5 cut(s) 19, 419, 462, 756, 1194
SsiI CCGC 2 cut(s) 65, 879
SspDI GGCGCC 1 cut(s) 917
SspMI CTAG 6 cut(s) 564, 722, 864, 963, 1118, 1127
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 2 cut(s) 77, 890
TaqI TCGA 4 cut(s) 387, 409, 738, 1081
TaqII GACCGA 1 cut(s) 779
TasI AATT 5 cut(s) 19, 419, 462, 756, 1194
TauI GCSGC 1 cut(s) 881
TfiI GAWTC 2 cut(s) 782, 1096
Tru1I TTAA 6 cut(s) 183, 192, 282, 330, 422, 575
Tru9I TTAA 6 cut(s) 183, 192, 282, 330, 422, 575
TseFI GTSAC 2 cut(s) 145, 760
TseI GCWGC 4 cut(s) 436, 443, 809, 1130
Tsp45I GTSAC 2 cut(s) 145, 760
TspDTI ATGAA 8 cut(s) 32, 205, 365, 375, 987, 1013, 1088, 1175
TspGWI ACGGA 2 cut(s) 349, 968
Vha464I CTTAAG 1 cut(s) 281
VpaK11BI GGWCC 2 cut(s) 275, 1028
XagI CCTNNNNNAGG 1 cut(s) 1116
XapI RAATTY 1 cut(s) 462
XhoI CTCGAG 2 cut(s) 408, 737
XmnI GAANNNNTTC 2 cut(s) 31, 324
XspI CTAG 6 cut(s) 564, 722, 864, 963, 1118, 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.