MD07G1231400.v1.1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009400
Physical Location & Seq
Forward (+)
30569340 .. 30572279
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1231400.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGACTGTGCCTGCAGCAGTTTCAGAAGCTCGTCTTTGCTCCTTCCATGGCGATCCCCATCTCCTCACCCGCACTCCTCCTTCCCTTCCAAGCTCAAATTGTTGAGACTGAAGCGTTTGGGCACGGCGAAGTATCAAAGGGTGGTCAGGAGCTCTTGCAGGTTCAACGAAAATGGCTCCGTAAATGGGTTTCCTGCCACACCCAACAAGCTTTTCATGCAGGAGGCAATTGGAGCTGAATATGGAGAAGGTTTTGAGACTTTTAGACCTGATGGACCTCTCAAGGTTGATGTGGATTTTTTGAATGACAAACTGCAAGAAGGTTTCCTTAAACGAATACGATATGCCATGAAACCGGATGAAGCTTATGGTCTTATATTCTCTTTTGACAATGTCGTGGCTGATACTCAAACTTTGAAACTAAATGCTTGGAAGCAGCTTGCTTCCGAAGAGGGAAAGGAACTTCCCGAAGATGGAGTTTTGCAAAGACGGATGCTTTATGCAGGTGCTGATCATGTGTTGCATAAGCTTTTACTGTGGGATAAAGCAGATGGAGAACTGGATAGATTGTCTTTGAAGTTTTCGCAATTATACTATGATAATCTTCTCAGGCTCAGTGAACCTGTGGAGGGGCTCAAAGAGTGGCTAGATGCTGTATCCACAGCTCGTATCCCTTGTGCTGTAGTTTCAAGTCTTGATCGGAGAAACATGACAGAAGCCCTAGAACGAATGGGACTCAAGAAGTACTTTCAGGCAATTGTGACAGAGGAAGATGGAATGGATTCAATAGCTCATAGGTTTCTTTCAGCAGCTATGAAGCTGGATCGGAAGCCATCCAAGTGTGTTGTGTTCGAGGATGAGCCTAGGGGTATAACCGCTGCTCACAACTGTACAATGATGGCTGTGGCATTGATTGGCGCCCACCCTGCGTATGACCTGGTGCAGGCCGATCTTGCAGTTGCTAGCTTTAATGAACTTTCCGTGATCAATCTTAGAAGACTATTTGCAAACAAGGGTTCTACATTCATGGACTTGCAGAAGCAGATTATAGAGAAATCTCCACCGAAGCGGAAACTTACGATAGATACTATTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

366

Amino Acids

41.2

Weight (kDa)

8.4

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_2 PF13419 126 - 302 2.7e-18 Haloacid dehalogenase-like hydrolase
Hydrolase PF00702 129 - 296 4.2e-10 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 494
Acc36I ACCTGC 2 cut(s) 150, 494
AccB1I GGYRCC 1 cut(s) 917
AciI CCGC 3 cut(s) 71, 876, 1069
AclWI GGATC 2 cut(s) 48, 831
AcuI CTGAAG 1 cut(s) 131
AcyI GRCGYC 1 cut(s) 918
AfaI GTAC 2 cut(s) 746, 892
AfiI CCNNNNNNNGG 4 cut(s) 186, 473, 629, 943
AgsI TTSAA 6 cut(s) 166, 304, 418, 577, 690, 786
AjnI CCWGG 1 cut(s) 936
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 2 cut(s) 100, 251
AlwI GGATC 2 cut(s) 48, 831
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 1 cut(s) 945
ApeKI GCWGC 4 cut(s) 15, 436, 809, 878
Asp700I GAANNNNTTC 1 cut(s) 781
AspA2I CCTAGG 1 cut(s) 863
AspLEI GCGC 1 cut(s) 920
AspS9I GGNCC 1 cut(s) 275
AsuHPI GGTGA 1 cut(s) 59
AsuNHI GCTAGC 1 cut(s) 962
AvaII GGWCC 1 cut(s) 275
AvrII CCTAGG 1 cut(s) 863
BaeGI GKGCMC 1 cut(s) 125
BanI GGYRCC 1 cut(s) 917
BanII GRGCYC 2 cut(s) 155, 636
BbsI GAAGAC 1 cut(s) 1003
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 4 cut(s) 27, 448, 821, 865
BccI CCATC 7 cut(s) 67, 266, 467, 545, 767, 841, 892
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 938
BciVI GTATCC 2 cut(s) 667, 680
BclI TGATCA 2 cut(s) 511, 984
BcoDI GTCTC 2 cut(s) 100, 251
BfaI CTAG 4 cut(s) 647, 722, 864, 963
BfmI CTRYAG 2 cut(s) 13, 681
BfoI RGCGCY 1 cut(s) 921
BfuAI ACCTGC 2 cut(s) 150, 494
BfuI GTATCC 2 cut(s) 667, 680
BisI GCNGC 4 cut(s) 16, 437, 810, 879
BlnI CCTAGG 1 cut(s) 863
BlsI GCNGC 4 cut(s) 17, 438, 811, 880
BmcAI AGTACT 1 cut(s) 746
Bme1390I CCNGG 1 cut(s) 938
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 2 cut(s) 178, 919
BmrFI CCNGG 1 cut(s) 938
BmsI GCATC 2 cut(s) 483, 640
BmtI GCTAGC 1 cut(s) 966
BpiI GAAGAC 1 cut(s) 1003
BpuEI CTTGAG 2 cut(s) 266, 722
BsaBI GATNNNNATC 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 918
BsaJI CCNNGG 2 cut(s) 47, 863
BsaWI WCCGGW 1 cut(s) 355
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 4 cut(s) 186, 473, 629, 943
Bse1I ACTGG 1 cut(s) 564
Bse8I GATNNNNATC 1 cut(s) 58
BseBI CCWGG 1 cut(s) 938
BseDI CCNNGG 2 cut(s) 47, 863
BseGI GGATG 4 cut(s) 364, 498, 833, 862
BseJI GATNNNNATC 1 cut(s) 58
BseLI CCNNNNNNNGG 4 cut(s) 186, 473, 629, 943
BseMII CTCAG 2 cut(s) 622, 628
BseNI ACTGG 1 cut(s) 564
BseRI GAGGAG 2 cut(s) 54, 67
BseSI GKGCMC 1 cut(s) 125
BseXI GCAGC 4 cut(s) 27, 448, 821, 865
BsgI GTGCAG 1 cut(s) 962
BshFI GGCC 1 cut(s) 947
BshNI GGYRCC 1 cut(s) 917
BsiHKAI GWGCWC 1 cut(s) 155
BsiSI CCGG 1 cut(s) 356
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 4 cut(s) 186, 473, 629, 943
BsmAI GTCTC 2 cut(s) 100, 251
BsmFI GGGAC 1 cut(s) 747
BsnI GGCC 1 cut(s) 947
Bsp1286I GDGCHC 3 cut(s) 125, 155, 636
Bsp1407I TGTACA 1 cut(s) 890
Bsp143I GATC 6 cut(s) 53, 511, 697, 823, 949, 984
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 3 cut(s) 71, 876, 1069
BspANI GGCC 1 cut(s) 947
BspCNI CTCAG 2 cut(s) 621, 627
BspLI GGNNCC 2 cut(s) 178, 919
BspMAI CTGCAG 1 cut(s) 17
BspMI ACCTGC 2 cut(s) 150, 494
BspOI GCTAGC 1 cut(s) 966
BspPI GGATC 2 cut(s) 48, 831
BspT107I GGYRCC 1 cut(s) 917
BsrGI TGTACA 1 cut(s) 890
BsrI ACTGG 1 cut(s) 564
BssECI CCNNGG 2 cut(s) 47, 863
BssMI GATC 6 cut(s) 53, 511, 697, 823, 949, 984
BssNI GRCGYC 1 cut(s) 918
BssT1I CCWWGG 2 cut(s) 47, 863
Bst2UI CCWGG 1 cut(s) 938
Bst4CI ACNGT 3 cut(s) 8, 537, 890
Bst6I CTCTTC 1 cut(s) 444
BstACI GRCGYC 1 cut(s) 918
BstAUI TGTACA 1 cut(s) 890
BstC8I GCNNGC 4 cut(s) 13, 441, 945, 964
BstDEI CTNAG 3 cut(s) 608, 614, 992
BstDSI CCRYGG 1 cut(s) 47
BstENI CCTNNNNNAGG 1 cut(s) 941
BstF5I GGATG 4 cut(s) 364, 498, 833, 862
BstH2I RGCGCY 1 cut(s) 921
BstHHI GCGC 1 cut(s) 920
BstKTI GATC 6 cut(s) 56, 514, 700, 826, 952, 987
BstMAI GTCTC 2 cut(s) 100, 251
BstMBI GATC 6 cut(s) 53, 511, 697, 823, 949, 984
BstMWI GCNNNNNNNGC 4 cut(s) 217, 233, 926, 953
BstNI CCWGG 1 cut(s) 938
BstSCI CCNGG 1 cut(s) 936
BstSFI CTRYAG 2 cut(s) 13, 681
BstSLI GKGCMC 1 cut(s) 125
BstV1I GCAGC 4 cut(s) 27, 448, 821, 865
BstV2I GAAGAC 1 cut(s) 1003
BsuI GTATCC 2 cut(s) 667, 680
BsuRI GGCC 1 cut(s) 947
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 4 cut(s) 364, 498, 833, 862
BtsIMutI CAGTG 1 cut(s) 622
BveI ACCTGC 2 cut(s) 150, 494
Cac8I GCNNGC 4 cut(s) 13, 441, 945, 964
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 1 cut(s) 920
Cfr13I GGNCC 1 cut(s) 275
CsiI ACCWGGT 1 cut(s) 936
Csp6I GTAC 2 cut(s) 745, 891
CviAII CATG 6 cut(s) 48, 217, 349, 515, 709, 1027
CviQI GTAC 2 cut(s) 745, 891
DdeI CTNAG 3 cut(s) 608, 614, 992
DinI GGCGCC 1 cut(s) 919
DpnI GATC 6 cut(s) 55, 513, 699, 825, 951, 986
DpnII GATC 6 cut(s) 53, 511, 697, 823, 949, 984
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
Ecl136II GAGCTC 1 cut(s) 153
Eco130I CCWWGG 2 cut(s) 47, 863
Eco24I GRGCYC 2 cut(s) 155, 636
Eco47I GGWCC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 153
EcoNI CCTNNNNNAGG 1 cut(s) 941
EcoRII CCWGG 1 cut(s) 936
EcoT14I CCWWGG 2 cut(s) 47, 863
EcoT38I GRGCYC 2 cut(s) 155, 636
EgeI GGCGCC 1 cut(s) 919
EheI GGCGCC 1 cut(s) 919
ErhI CCWWGG 2 cut(s) 47, 863
FaeI CATG 6 cut(s) 51, 220, 352, 518, 712, 1030
FalI AAGNNNNNCTT 6 cut(s) 19, 51, 312, 344, 731, 763
FaqI GGGAC 1 cut(s) 747
FatI CATG 6 cut(s) 47, 216, 348, 514, 708, 1026
FauI CCCGC 1 cut(s) 78
FbaI TGATCA 2 cut(s) 511, 984
Fnu4HI GCNGC 4 cut(s) 16, 437, 810, 879
FokI GGATG 4 cut(s) 371, 505, 820, 869
FriOI GRGCYC 2 cut(s) 155, 636
Fsp4HI GCNGC 4 cut(s) 16, 437, 810, 879
FspBI CTAG 4 cut(s) 647, 722, 864, 963
GlaI GCGC 1 cut(s) 919
GluI GCNGC 4 cut(s) 16, 437, 810, 879
HaeII RGCGCY 1 cut(s) 921
HaeIII GGCC 1 cut(s) 947
HapII CCGG 1 cut(s) 356
HhaI GCGC 1 cut(s) 920
Hin1I GRCGYC 1 cut(s) 918
Hin1II CATG 6 cut(s) 51, 220, 352, 518, 712, 1030
Hin6I GCGC 1 cut(s) 918
HinP1I GCGC 1 cut(s) 918
HindIII AAGCTT 3 cut(s) 209, 363, 527
HinfI GANTC 2 cut(s) 735, 782
HpaII CCGG 1 cut(s) 356
HphI GGTGA 1 cut(s) 59
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 5 cut(s) 26, 448, 702, 828, 1097
Hpy188III TCNNGA 4 cut(s) 148, 467, 695, 739
Hpy8I GTNNAC 1 cut(s) 620
HpyAV CCTTC 5 cut(s) 53, 91, 96, 242, 314
HpyCH4III ACNGT 3 cut(s) 8, 537, 890
HpyF10VI GCNNNNNNNGC 4 cut(s) 217, 233, 926, 953
HpyF3I CTNAG 3 cut(s) 608, 614, 992
Hsp92I GRCGYC 1 cut(s) 918
Hsp92II CATG 6 cut(s) 51, 220, 352, 518, 712, 1030
HspAI GCGC 1 cut(s) 918
KasI GGCGCC 1 cut(s) 917
Ksp22I TGATCA 2 cut(s) 511, 984
Kzo9I GATC 6 cut(s) 53, 511, 697, 823, 949, 984
LmnI GCTCC 4 cut(s) 45, 150, 182, 233
Lsp1109I GCAGC 4 cut(s) 27, 448, 821, 865
LweI GCATC 2 cut(s) 483, 640
MabI ACCWGGT 1 cut(s) 936
MaeI CTAG 4 cut(s) 647, 722, 864, 963
MaeIII GTNAC 1 cut(s) 760
MalI GATC 6 cut(s) 55, 513, 699, 825, 951, 986
MboI GATC 6 cut(s) 53, 511, 697, 823, 949, 984
MboII GAAGA 5 cut(s) 461, 482, 596, 782, 1008
MfeI CAATTG 2 cut(s) 228, 756
MhlI GDGCHC 3 cut(s) 125, 155, 636
MluCI AATT 4 cut(s) 98, 228, 587, 756
Mly113I GGCGCC 1 cut(s) 918
MlyI GAGTC 1 cut(s) 729
MnlI CCTC 8 cut(s) 75, 88, 217, 288, 445, 622, 760, 847
MroXI GAANNNNTTC 1 cut(s) 781
MseI TTAA 2 cut(s) 330, 969
MslI CAYNNNNRTG 1 cut(s) 838
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 938
MunI CAATTG 2 cut(s) 228, 756
MvaI CCWGG 1 cut(s) 938
MwoI GCNNNNNNNGC 4 cut(s) 217, 233, 926, 953
NarI GGCGCC 1 cut(s) 918
NcoI CCATGG 1 cut(s) 47
NdeII GATC 6 cut(s) 53, 511, 697, 823, 949, 984
NheI GCTAGC 1 cut(s) 962
NlaIII CATG 6 cut(s) 51, 220, 352, 518, 712, 1030
NlaIV GGNNCC 2 cut(s) 178, 919
NmuCI GTSAC 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 781
PfeI GAWTC 1 cut(s) 782
PflFI GACNNNGTC 1 cut(s) 392
PkrI GCNGC 4 cut(s) 17, 438, 811, 880
PleI GAGTC 1 cut(s) 729
PluTI GGCGCC 1 cut(s) 921
PpsI GAGTC 1 cut(s) 729
Psp124BI GAGCTC 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 936
PspGI CCWGG 1 cut(s) 936
PspN4I GGNNCC 2 cut(s) 178, 919
PspPI GGNCC 1 cut(s) 275
PstI CTGCAG 1 cut(s) 17
PstNI CAGNNNCTG 1 cut(s) 509
PsyI GACNNNGTC 1 cut(s) 392
RsaI GTAC 2 cut(s) 746, 892
RsaNI GTAC 2 cut(s) 745, 891
RseI CAYNNNNRTG 1 cut(s) 838
SacI GAGCTC 1 cut(s) 155
SaqAI TTAA 2 cut(s) 330, 969
SatI GCNGC 4 cut(s) 16, 437, 810, 879
Sau3AI GATC 6 cut(s) 53, 511, 697, 823, 949, 984
Sau96I GGNCC 1 cut(s) 275
ScaI AGTACT 1 cut(s) 746
SchI GAGTC 1 cut(s) 729
ScrFI CCNGG 1 cut(s) 938
SduI GDGCHC 3 cut(s) 125, 155, 636
SexAI ACCWGGT 1 cut(s) 936
SfaNI GCATC 2 cut(s) 483, 640
SfcI CTRYAG 2 cut(s) 13, 681
SfoI GGCGCC 1 cut(s) 919
SinI GGWCC 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 838
SmlI CTYRAG 2 cut(s) 281, 737
SmoI CTYRAG 2 cut(s) 281, 737
Sse9I AATT 4 cut(s) 98, 228, 587, 756
SsiI CCGC 3 cut(s) 71, 876, 1069
SspDI GGCGCC 1 cut(s) 917
SspMI CTAG 4 cut(s) 647, 722, 864, 963
SstI GAGCTC 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 936
StyI CCWWGG 2 cut(s) 47, 863
TaaI ACNGT 3 cut(s) 8, 537, 890
TaqI TCGA 1 cut(s) 852
TasI AATT 4 cut(s) 98, 228, 587, 756
TatI WGTACW 2 cut(s) 744, 890
TfiI GAWTC 1 cut(s) 782
Tru1I TTAA 2 cut(s) 330, 969
Tru9I TTAA 2 cut(s) 330, 969
TscAI CASTG 1 cut(s) 622
TseFI GTSAC 1 cut(s) 760
TseI GCWGC 4 cut(s) 15, 436, 809, 878
Tsp45I GTSAC 1 cut(s) 760
TspDTI ATGAA 6 cut(s) 205, 365, 375, 830, 987, 1015
TspGWI ACGGA 3 cut(s) 169, 505, 970
TspRI CASTG 1 cut(s) 622
Tth111I GACNNNGTC 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 275
XagI CCTNNNNNAGG 1 cut(s) 941
XmaJI CCTAGG 1 cut(s) 863
XmnI GAANNNNTTC 1 cut(s) 781
XspI CTAG 4 cut(s) 647, 722, 864, 963
ZrmI AGTACT 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.