MD01G1177200.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015285
Physical Location & Seq
Forward (+)
27797664 .. 27798401
738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1177200.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGTAATTAGCTTCCCCTTCTGCCCTCACTTCAAAATGTCTACACATGAACAAGATGATTCAACTCTTAAGCTCATCCGCCAACACCTCTTAGAAGACTTCACCTTCACAGATTCCTTCATTTCTCATCTCAATTTCGAGATTACCCCTGATGATATCTCATATTTCGAGTTCCAACCAGTCAAACCCGAGTACTCATCCCTCTCTGAATCAGGGTCCAGCTCCCCCATTTCAACCCCAAATTACCACAGCCCCCAACTCTCAAATTCCGAAACGAAACCCGAAAGCGTAAACTTACCATCCATGGAAGTAGAGCCCCTGAATTTGAGCTCACAATGCTCACCAAAGAGAAAATATAGTACTTGTCAAACTTCCAGTTCAAAAGATGAATTACAACAAGTTTCAGGCTCTGGTGATGTGTTAAGGCATTACAGGGGAGTCCGGCGGAGGCCGTGGGGCAAGTACGCGGCGGAGATACGTGATCCGGCCCGGAAAGGAACCCGGGTTTGGCTAGGGACATTTGACACTGATGTTGACGCTGCCAAGGCCTATGATTGTGCAGCATTTAAGCTGAGGGGCAGGAAAGCAATACTGAATTTTCCGTTGGAGGCCGGACTGTCCGAACCGCCAGTGAACACGGTGCGGAAGAGGAGGAGATCGGTAAAGCAGGAGGAGGTAACAGAGACTGAAGAATTGGAGCTTCAAAGTTGGGTGGTTTGGGAAGGAGAAGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

246

Amino Acids

27.83

Weight (kDa)

5.75

Isoelectric Point (pI)

69.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 143 - 193 2.5e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07580 AT5G61590
fragaria_vesca FvH4_7g26940
malus_domestica MD01G1177200.v1.1 MD07G1248700.v1.1
prunus_persica Prupe.2G272500_v2.0.a1
pyrus_communis pycom07g22210
rosa_chinensis RchiOBHm_Chr1g0373641
rosa_laevigata RLG00000026810
rosa_multiflora Rmu_sc0013090.1_g000002
rosa_roxburghii Rroxscaffold_4G00284090
rosa_rugosa Rorug01G0378700
rosa_samantha Rh1AG388200 Rh1BG352500 Rh1CG365300
rosa_wichuraiana Rw1G034710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 41
AccII CGCG 1 cut(s) 467
AciI CCGC 6 cut(s) 79, 445, 467, 470, 626, 643
AclWI GGATC 1 cut(s) 476
AcsI RAATTY 3 cut(s) 265, 322, 595
AcuI CTGAAG 1 cut(s) 708
AfaI GTAC 3 cut(s) 194, 361, 464
AfiI CCNNNNNNNGG 1 cut(s) 507
AflII CTTAAG 1 cut(s) 68
AgsI TTSAA 5 cut(s) 34, 63, 234, 381, 704
AjuI GAANNNNNNNTTGG 4 cut(s) 490, 522, 587, 619
AluBI AGCT 6 cut(s) 12, 73, 222, 330, 571, 700
AluI AGCT 6 cut(s) 12, 73, 222, 330, 571, 700
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 1 cut(s) 677
AlwI GGATC 1 cut(s) 476
AlwNI CAGNNNCTG 2 cut(s) 410, 686
Ama87I CYCGRG 2 cut(s) 188, 501
AoxI GGCC 4 cut(s) 449, 486, 546, 609
ApeKI GCWGC 2 cut(s) 539, 560
ApoI RAATTY 3 cut(s) 265, 322, 595
AspS9I GGNCC 2 cut(s) 216, 487
AsuC2I CCSGG 3 cut(s) 490, 502, 503
AsuHPI GGTGA 3 cut(s) 94, 333, 425
AvaI CYCGRG 2 cut(s) 188, 501
AvaII GGWCC 1 cut(s) 216
BanII GRGCYC 2 cut(s) 318, 332
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 332
BbvCI CCTCAGC 1 cut(s) 572
BbvI GCAGC 2 cut(s) 526, 572
BccI CCATC 1 cut(s) 307
BceAI ACGGC 1 cut(s) 436
BcnI CCSGG 3 cut(s) 490, 502, 503
BcoDI GTCTC 1 cut(s) 677
BfaI CTAG 1 cut(s) 512
BfrI CTTAAG 1 cut(s) 68
BisI GCNGC 3 cut(s) 468, 540, 561
BlsI GCNGC 3 cut(s) 469, 541, 562
BmcAI AGTACT 2 cut(s) 194, 361
Bme1390I CCNGG 3 cut(s) 490, 502, 503
Bme18I GGWCC 1 cut(s) 216
BmeT110I CYCGRG 2 cut(s) 188, 501
BmgT120I GGNCC 2 cut(s) 216, 487
BmiI GGNNCC 2 cut(s) 217, 499
BmrFI CCNGG 3 cut(s) 490, 502, 503
BpiI GAAGAC 1 cut(s) 102
Bpu10I CCTNAGC 1 cut(s) 572
BpuMI CCSGG 3 cut(s) 490, 502, 503
BsaAI YACGTR 1 cut(s) 479
BsaJI CCNNGG 4 cut(s) 303, 452, 501, 543
Bsc4I CCNNNNNNNGG 1 cut(s) 507
Bse1I ACTGG 3 cut(s) 179, 375, 629
BseDI CCNNGG 4 cut(s) 303, 452, 501, 543
BseGI GGATG 3 cut(s) 75, 197, 299
BseLI CCNNNNNNNGG 1 cut(s) 507
BseMII CTCAG 1 cut(s) 563
BseNI ACTGG 3 cut(s) 179, 375, 629
BseRI GAGGAG 3 cut(s) 664, 667, 686
BseXI GCAGC 2 cut(s) 526, 572
BsgI GTGCAG 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 467
BshFI GGCC 4 cut(s) 451, 488, 548, 611
BsiHKAI GWGCWC 1 cut(s) 332
BsiHKCI CYCGRG 2 cut(s) 188, 501
BsiSI CCGG 5 cut(s) 442, 485, 490, 502, 612
BslFI GGGAC 1 cut(s) 529
BslI CCNNNNNNNGG 1 cut(s) 507
BsmAI GTCTC 1 cut(s) 677
BsmFI GGGAC 1 cut(s) 529
BsnI GGCC 4 cut(s) 451, 488, 548, 611
BsoBI CYCGRG 2 cut(s) 188, 501
Bsp1286I GDGCHC 2 cut(s) 318, 332
Bsp143I GATC 2 cut(s) 481, 656
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 6 cut(s) 79, 445, 467, 470, 626, 643
BspANI GGCC 4 cut(s) 451, 488, 548, 611
BspCNI CTCAG 1 cut(s) 564
BspFNI CGCG 1 cut(s) 467
BspLI GGNNCC 2 cut(s) 217, 499
BspPI GGATC 1 cut(s) 476
BspTI CTTAAG 1 cut(s) 68
BsrI ACTGG 3 cut(s) 179, 375, 629
BssECI CCNNGG 4 cut(s) 303, 452, 501, 543
BssMI GATC 2 cut(s) 481, 656
BssT1I CCWWGG 2 cut(s) 303, 543
Bst4CI ACNGT 2 cut(s) 618, 640
Bst6I CTCTTC 1 cut(s) 641
BstAFI CTTAAG 1 cut(s) 68
BstBAI YACGTR 1 cut(s) 479
BstDEI CTNAG 2 cut(s) 91, 572
BstDSI CCRYGG 2 cut(s) 303, 452
BstF5I GGATG 3 cut(s) 75, 197, 299
BstFNI CGCG 1 cut(s) 467
BstKTI GATC 2 cut(s) 484, 659
BstMAI GTCTC 1 cut(s) 677
BstMBI GATC 2 cut(s) 481, 656
BstMWI GCNNNNNNNGC 2 cut(s) 336, 545
BstSCI CCNGG 3 cut(s) 488, 500, 501
BstUI CGCG 1 cut(s) 467
BstV1I GCAGC 2 cut(s) 526, 572
BstV2I GAAGAC 1 cut(s) 102
BsuRI GGCC 4 cut(s) 451, 488, 548, 611
BtgI CCRYGG 2 cut(s) 303, 452
BtsCI GGATG 3 cut(s) 75, 197, 299
BtsIMutI CAGTG 2 cut(s) 525, 636
CaiI CAGNNNCTG 2 cut(s) 410, 686
Cfr13I GGNCC 2 cut(s) 216, 487
Cfr9I CCCGGG 1 cut(s) 501
CseI GACGC 1 cut(s) 545
Csp6I GTAC 3 cut(s) 193, 360, 463
CviAII CATG 2 cut(s) 47, 304
CviQI GTAC 3 cut(s) 193, 360, 463
DdeI CTNAG 2 cut(s) 91, 572
DpnI GATC 2 cut(s) 483, 658
DpnII GATC 2 cut(s) 481, 656
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
EciI GGCGGA 3 cut(s) 68, 460, 485
Ecl136II GAGCTC 1 cut(s) 330
Eco130I CCWWGG 2 cut(s) 303, 543
Eco147I AGGCCT 1 cut(s) 548
Eco24I GRGCYC 2 cut(s) 318, 332
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 216
Eco53kI GAGCTC 1 cut(s) 330
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 2 cut(s) 188, 501
EcoICRI GAGCTC 1 cut(s) 330
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 2 cut(s) 303, 543
EcoT38I GRGCYC 2 cut(s) 318, 332
ErhI CCWWGG 2 cut(s) 303, 543
FaeI CATG 2 cut(s) 50, 307
FaiI YATR 5 cut(s) 48, 163, 305, 357, 552
FaqI GGGAC 1 cut(s) 529
FatI CATG 2 cut(s) 46, 303
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 3 cut(s) 468, 540, 561
FokI GGATG 3 cut(s) 62, 184, 286
FriOI GRGCYC 2 cut(s) 318, 332
Fsp4HI GCNGC 3 cut(s) 468, 540, 561
FspBI CTAG 1 cut(s) 512
GluI GCNGC 3 cut(s) 468, 540, 561
HaeIII GGCC 4 cut(s) 451, 488, 548, 611
HapII CCGG 5 cut(s) 442, 485, 490, 502, 612
HgaI GACGC 1 cut(s) 545
Hin1II CATG 2 cut(s) 50, 307
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 4 cut(s) 59, 113, 209, 438
HpaII CCGG 5 cut(s) 442, 485, 490, 502, 612
HphI GGTGA 3 cut(s) 94, 333, 425
Hpy166II GTNNAC 4 cut(s) 42, 292, 535, 634
Hpy188I TCNGA 3 cut(s) 208, 271, 622
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 4 cut(s) 42, 292, 535, 634
HpyAV CCTTC 5 cut(s) 28, 115, 127, 716, 722
HpyCH4III ACNGT 2 cut(s) 618, 640
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 1 cut(s) 560
HpyF10VI GCNNNNNNNGC 2 cut(s) 336, 545
HpyF3I CTNAG 2 cut(s) 91, 572
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 2 cut(s) 50, 307
Kzo9I GATC 2 cut(s) 481, 656
LmnI GCTCC 2 cut(s) 227, 697
Lsp1109I GCAGC 2 cut(s) 526, 572
MaeI CTAG 1 cut(s) 512
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 1 cut(s) 676
MalI GATC 2 cut(s) 483, 658
MboI GATC 2 cut(s) 481, 656
MboII GAAGA 3 cut(s) 107, 658, 701
MhlI GDGCHC 2 cut(s) 318, 332
MluCI AATT 8 cut(s) 6, 133, 241, 265, 322, 389, 595, 692
MlyI GAGTC 1 cut(s) 447
MmeI TCCRAC 2 cut(s) 199, 585
MseI TTAA 3 cut(s) 69, 422, 567
MspCI CTTAAG 1 cut(s) 68
MspI CCGG 5 cut(s) 442, 485, 490, 502, 612
MspR9I CCNGG 3 cut(s) 490, 502, 503
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 2 cut(s) 336, 545
NciI CCSGG 3 cut(s) 490, 502, 503
NcoI CCATGG 1 cut(s) 303
NdeII GATC 2 cut(s) 481, 656
NlaIII CATG 2 cut(s) 50, 307
NlaIV GGNNCC 2 cut(s) 217, 499
PceI AGGCCT 1 cut(s) 548
PfeI GAWTC 3 cut(s) 59, 113, 209
PkrI GCNGC 3 cut(s) 469, 541, 562
PleI GAGTC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 446
Ppu21I YACGTR 1 cut(s) 479
Psp124BI GAGCTC 1 cut(s) 332
PspN4I GGNNCC 2 cut(s) 217, 499
PspPI GGNCC 2 cut(s) 216, 487
PstNI CAGNNNCTG 2 cut(s) 410, 686
RsaI GTAC 3 cut(s) 194, 361, 464
RsaNI GTAC 3 cut(s) 193, 360, 463
SacI GAGCTC 1 cut(s) 332
SaqAI TTAA 3 cut(s) 69, 422, 567
SatI GCNGC 3 cut(s) 468, 540, 561
Sau3AI GATC 2 cut(s) 481, 656
Sau96I GGNCC 2 cut(s) 216, 487
ScaI AGTACT 2 cut(s) 194, 361
SchI GAGTC 1 cut(s) 447
ScrFI CCNGG 3 cut(s) 490, 502, 503
SduI GDGCHC 2 cut(s) 318, 332
SinI GGWCC 1 cut(s) 216
SmaI CCCGGG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 8 cut(s) 6, 133, 241, 265, 322, 389, 595, 692
SseBI AGGCCT 1 cut(s) 548
SsiI CCGC 6 cut(s) 79, 445, 467, 470, 626, 643
SspMI CTAG 1 cut(s) 512
SstI GAGCTC 1 cut(s) 332
StuI AGGCCT 1 cut(s) 548
StyD4I CCNGG 3 cut(s) 488, 500, 501
StyI CCWWGG 2 cut(s) 303, 543
TaaI ACNGT 2 cut(s) 618, 640
TaiI ACGT 1 cut(s) 481
TaqI TCGA 2 cut(s) 138, 168
TasI AATT 8 cut(s) 6, 133, 241, 265, 322, 389, 595, 692
TatI WGTACW 2 cut(s) 192, 359
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 3 cut(s) 59, 113, 209
Tru1I TTAA 3 cut(s) 69, 422, 567
Tru9I TTAA 3 cut(s) 69, 422, 567
TscAI CASTG 2 cut(s) 532, 636
TseI GCWGC 2 cut(s) 539, 560
TspDTI ATGAA 3 cut(s) 63, 109, 402
TspGWI ACGGA 1 cut(s) 591
TspMI CCCGGG 1 cut(s) 501
TspRI CASTG 2 cut(s) 532, 636
Vha464I CTTAAG 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 216
XapI RAATTY 3 cut(s) 265, 322, 595
XmaI CCCGGG 1 cut(s) 501
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 1 cut(s) 512
ZrmI AGTACT 2 cut(s) 194, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.