Rh1BG352500

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0015285
Physical Location & Seq
Forward (+)
48477890 .. 48480045
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG352500.1

Sequence Viewer

Length: 642 bp
ATGGCAGCAACCCAAGAACAAGATGAGTCAACTCTCGAACTCATACGCCAACATCTCTTAGGAGACTTCACCTTCACAGACAGCTTCATCACCAATCTCAACTACGAACAAAGCCCAGAAATCTCCTACTTCCAACCCATCAAATCCGAGTACTCCATATCCGAGTCCGAGTCCACCTCCCCCGTTTCAAACTACCTCAACCCGAAACCCGAACCCATAACCGAAGAAGAACCCATGAGCTCCAAGAGCGAGCCCAAGAGAAAGTGGAGTTCTTCCAACTCCAGCTCATCGACTGGCGAGGCCGCCAGGCATTACAGGGGAGTCCGGAGAAGGCCGTGGGGCAAGTTTGCGGCGGAGATTCGTGACCCGGCCCGGAAGGGGAGCCGGGTCTGGCTGGGGACGTTTGACACTGATGTGGATGCTGCTAAGGCCTATGATTTTGCTGCATTCAAGCTCAGGGGAAGGAAAGCTATACTGAATTTTCCGCTGGAGGCCGGAGAGTCCGACCCGCCAAGTAATACGGGTCGGAAAAGGAGGAAGTCAGTGAAGCAAGAGGAGGAGGAGGAGGAGGCGGCGCCGGCTCCGGAGGCGGAAGAGGTGGAGGTTCAAAGTTGGGTGGATTGGGATGGAGAAGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

213

Amino Acids

24.07

Weight (kDa)

4.82

Isoelectric Point (pI)

77.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 104 - 154 1.1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07580 AT5G61590
fragaria_vesca FvH4_7g26940
malus_domestica MD01G1177200.v1.1 MD07G1248700.v1.1
prunus_persica Prupe.2G272500_v2.0.a1
pyrus_communis pycom07g22210
rosa_chinensis RchiOBHm_Chr1g0373641
rosa_laevigata RLG00000026810
rosa_multiflora Rmu_sc0013090.1_g000002
rosa_roxburghii Rroxscaffold_4G00284090
rosa_rugosa Rorug01G0378700
rosa_samantha Rh1AG388200 Rh1BG352500 Rh1CG365300
rosa_wichuraiana Rw1G034710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 574
AccIII TCCGGA 2 cut(s) 324, 583
AciI CCGC 7 cut(s) 303, 350, 353, 485, 509, 572, 590
AcsI RAATTY 1 cut(s) 478
AcyI GRCGYC 1 cut(s) 575
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 1 cut(s) 378
AgsI TTSAA 3 cut(s) 189, 451, 608
AjnI CCWGG 1 cut(s) 305
AleI CACNNNNGTG 1 cut(s) 413
AluBI AGCT 5 cut(s) 84, 240, 285, 454, 470
AluI AGCT 5 cut(s) 84, 240, 285, 454, 470
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 57
Aor13HI TCCGGA 2 cut(s) 324, 583
AoxI GGCC 5 cut(s) 300, 332, 369, 429, 492
ApeKI GCWGC 3 cut(s) 5, 422, 443
ApoI RAATTY 1 cut(s) 478
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 1 cut(s) 370
AsuC2I CCSGG 3 cut(s) 368, 373, 386
AsuHPI GGTGA 2 cut(s) 61, 82
BanI GGYRCC 1 cut(s) 574
BanII GRGCYC 2 cut(s) 242, 255
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 3 cut(s) 17, 409, 430
BccI CCATC 2 cut(s) 146, 620
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 1 cut(s) 307
BcnI CCSGG 3 cut(s) 368, 373, 386
BcoDI GTCTC 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 578
BisI GCNGC 6 cut(s) 6, 303, 351, 423, 444, 573
BlsI GCNGC 6 cut(s) 7, 304, 352, 424, 445, 574
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 4 cut(s) 307, 368, 373, 386
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 3 cut(s) 383, 576, 582
BmrFI CCNGG 4 cut(s) 307, 368, 373, 386
BmsI GCATC 2 cut(s) 409, 625
BplI GAGNNNNNCTC 2 cut(s) 161, 193
BpmI CTGGAG 2 cut(s) 265, 509
Bpu10I CCTNAGC 2 cut(s) 426, 455
BpuMI CCSGG 3 cut(s) 368, 373, 386
BsaHI GRCGYC 1 cut(s) 575
BsaJI CCNNGG 1 cut(s) 335
BsaWI WCCGGW 2 cut(s) 324, 583
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse118I RCCGGY 1 cut(s) 577
Bse1I ACTGG 1 cut(s) 298
BseAI TCCGGA 2 cut(s) 324, 583
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 335
BseGI GGATG 2 cut(s) 424, 631
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 469
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 5 cut(s) 569, 572, 575, 578, 581
BseXI GCAGC 3 cut(s) 17, 409, 430
BseYI CCCAGC 1 cut(s) 394
BshFI GGCC 5 cut(s) 302, 334, 371, 431, 494
BshNI GGYRCC 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 242
BsiSI CCGG 7 cut(s) 325, 368, 373, 385, 495, 578, 584
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 1 cut(s) 378
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 412
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 5 cut(s) 302, 334, 371, 431, 494
Bsp1286I GDGCHC 2 cut(s) 242, 255
Bsp13I TCCGGA 2 cut(s) 324, 583
BspACI CCGC 7 cut(s) 303, 350, 353, 485, 509, 572, 590
BspANI GGCC 5 cut(s) 302, 334, 371, 431, 494
BspCNI CTCAG 1 cut(s) 468
BspEI TCCGGA 2 cut(s) 324, 583
BspLI GGNNCC 3 cut(s) 383, 576, 582
BspT107I GGYRCC 1 cut(s) 574
BsrFI RCCGGY 1 cut(s) 577
BsrI ACTGG 1 cut(s) 298
BssAI RCCGGY 1 cut(s) 577
BssECI CCNNGG 1 cut(s) 335
BssNI GRCGYC 1 cut(s) 575
Bst2UI CCWGG 1 cut(s) 307
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 1 cut(s) 575
BstC8I GCNNGC 2 cut(s) 251, 579
BstDEI CTNAG 3 cut(s) 58, 426, 455
BstDSI CCRYGG 1 cut(s) 335
BstF5I GGATG 2 cut(s) 424, 631
BstH2I RGCGCY 1 cut(s) 578
BstHHI GCGC 1 cut(s) 577
BstMAI GTCTC 1 cut(s) 57
BstMWI GCNNNNNNNGC 4 cut(s) 246, 428, 578, 587
BstNI CCWGG 1 cut(s) 307
BstSCI CCNGG 4 cut(s) 305, 366, 371, 384
BstV1I GCAGC 3 cut(s) 17, 409, 430
BsuRI GGCC 5 cut(s) 302, 334, 371, 431, 494
BtgI CCRYGG 1 cut(s) 335
BtsCI GGATG 2 cut(s) 424, 631
BtsIMutI CAGTG 2 cut(s) 408, 549
Cac8I GCNNGC 2 cut(s) 251, 579
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 1 cut(s) 577
Cfr13I GGNCC 1 cut(s) 370
Csp6I GTAC 1 cut(s) 151
CviAII CATG 1 cut(s) 235
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 3 cut(s) 58, 426, 455
DinI GGCGCC 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
EciI GGCGGA 2 cut(s) 368, 605
Ecl136II GAGCTC 1 cut(s) 240
Eco147I AGGCCT 1 cut(s) 431
Eco24I GRGCYC 2 cut(s) 242, 255
Eco53kI GAGCTC 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 305
EcoT38I GRGCYC 2 cut(s) 242, 255
EgeI GGCGCC 1 cut(s) 576
EheI GGCGCC 1 cut(s) 576
FaeI CATG 1 cut(s) 238
FaiI YATR 6 cut(s) 44, 158, 218, 236, 435, 473
FaqI GGGAC 1 cut(s) 412
FatI CATG 1 cut(s) 234
FauI CCCGC 1 cut(s) 516
Fnu4HI GCNGC 6 cut(s) 6, 303, 351, 423, 444, 573
FokI GGATG 2 cut(s) 431, 638
FriOI GRGCYC 2 cut(s) 242, 255
Fsp4HI GCNGC 6 cut(s) 6, 303, 351, 423, 444, 573
GlaI GCGC 1 cut(s) 576
GluI GCNGC 6 cut(s) 6, 303, 351, 423, 444, 573
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 2 cut(s) 265, 509
HaeII RGCGCY 1 cut(s) 578
HaeIII GGCC 5 cut(s) 302, 334, 371, 431, 494
HapII CCGG 7 cut(s) 325, 368, 373, 385, 495, 578, 584
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 575
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 6 cut(s) 26, 164, 170, 321, 358, 500
HpaII CCGG 7 cut(s) 325, 368, 373, 385, 495, 578, 584
HphI GGTGA 2 cut(s) 61, 82
Hpy166II GTNNAC 2 cut(s) 30, 174
Hpy188I TCNGA 5 cut(s) 148, 163, 169, 505, 528
Hpy188III TCNNGA 4 cut(s) 35, 325, 362, 584
Hpy8I GTNNAC 2 cut(s) 30, 174
HpyAV CCTTC 4 cut(s) 82, 324, 370, 456
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 1 cut(s) 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 428, 578, 587
HpyF3I CTNAG 3 cut(s) 58, 426, 455
HpySE526I ACGT 1 cut(s) 401
Hsp92I GRCGYC 1 cut(s) 575
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 1 cut(s) 575
KasI GGCGCC 1 cut(s) 574
Kpn2I TCCGGA 2 cut(s) 324, 583
KroI GCCGGC 1 cut(s) 577
KroNI GCCGGC 1 cut(s) 579
LmnI GCTCC 3 cut(s) 245, 381, 586
Lsp1109I GCAGC 3 cut(s) 17, 409, 430
LweI GCATC 2 cut(s) 409, 625
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 1 cut(s) 362
MboII GAAGA 4 cut(s) 236, 239, 264, 605
MhlI GDGCHC 2 cut(s) 242, 255
MluCI AATT 1 cut(s) 478
Mly113I GGCGCC 1 cut(s) 575
MlyI GAGTC 5 cut(s) 35, 173, 179, 330, 509
MmeI TCCRAC 4 cut(s) 157, 300, 506, 528
MroI TCCGGA 2 cut(s) 324, 583
MroNI GCCGGC 1 cut(s) 577
MslI CAYNNNNRTG 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 487
MspI CCGG 7 cut(s) 325, 368, 373, 385, 495, 578, 584
MspR9I CCNGG 4 cut(s) 307, 368, 373, 386
Mva1269I GAATGC 1 cut(s) 446
MvaI CCWGG 1 cut(s) 307
MwoI GCNNNNNNNGC 4 cut(s) 246, 428, 578, 587
NaeI GCCGGC 1 cut(s) 579
NarI GGCGCC 1 cut(s) 575
NciI CCSGG 3 cut(s) 368, 373, 386
NgoMIV GCCGGC 1 cut(s) 577
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 3 cut(s) 383, 576, 582
NmuCI GTSAC 1 cut(s) 362
OliI CACNNNNGTG 1 cut(s) 413
PceI AGGCCT 1 cut(s) 431
PctI GAATGC 1 cut(s) 446
PdiI GCCGGC 1 cut(s) 579
PfeI GAWTC 1 cut(s) 358
PkrI GCNGC 6 cut(s) 7, 304, 352, 424, 445, 574
PleI GAGTC 5 cut(s) 34, 172, 178, 329, 508
PluTI GGCGCC 1 cut(s) 578
PpsI GAGTC 5 cut(s) 34, 172, 178, 329, 508
Psp124BI GAGCTC 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 305
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 3 cut(s) 383, 576, 582
PspPI GGNCC 1 cut(s) 370
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 1 cut(s) 242
SatI GCNGC 6 cut(s) 6, 303, 351, 423, 444, 573
Sau96I GGNCC 1 cut(s) 370
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 5 cut(s) 35, 173, 179, 330, 509
ScrFI CCNGG 4 cut(s) 307, 368, 373, 386
SduI GDGCHC 2 cut(s) 242, 255
SfaNI GCATC 2 cut(s) 409, 625
SfoI GGCGCC 1 cut(s) 576
SmiMI CAYNNNNRTG 1 cut(s) 413
Sse9I AATT 1 cut(s) 478
SseBI AGGCCT 1 cut(s) 431
SsiI CCGC 7 cut(s) 303, 350, 353, 485, 509, 572, 590
SspDI GGCGCC 1 cut(s) 574
SstI GAGCTC 1 cut(s) 242
StuI AGGCCT 1 cut(s) 431
StyD4I CCNGG 4 cut(s) 305, 366, 371, 384
TaiI ACGT 1 cut(s) 404
TaqI TCGA 2 cut(s) 36, 290
TasI AATT 1 cut(s) 478
TatI WGTACW 1 cut(s) 150
TauI GCSGC 3 cut(s) 305, 353, 575
TfiI GAWTC 1 cut(s) 358
TscAI CASTG 2 cut(s) 415, 549
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 3 cut(s) 5, 422, 443
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 1 cut(s) 76
TspRI CASTG 2 cut(s) 415, 549
XapI RAATTY 1 cut(s) 478
ZrmI AGTACT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.