Rorug01G0378700

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015285
Physical Location & Seq
Forward (+)
49187583 .. 49192333
4751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0378700.1

Sequence Viewer

Length: 1668 bp
ATGCCACAAAATAGCAATACAAACCCAATCACCTCACATTCGGGTTCAGATCCCAACACCAACCAACTCGCCATGGTTCAAGATGGCGACGCGTCTTCGCAAACTCCATACGAGGCGGCACTGGAGGCTCTTTCCTCCTTGATCACAAAGCGCAGCCGCGCCGATAAGAGCAACAAAGGCGACCGCTTCGACCTCCTCTTTGACTATCTCAGAATTCTGGAATTAGAGGAGCCGATTGCGCAGATGAAGATCATTCATGTGGCTGGTACCAAAGGAAAGGGATCCACATGCACCTTCTCTGAATCAATACTACGTCATTGTGGCTTCCGCACTGGACTTTTCACATCTCCTCATCTCATTGATGTTCGCGAAAGATTTCGGTTGGATGGAGTGGACATCAGTGAAGAGAAATTCTTGGAGTATTTCTGGTGGTCTTTTAATAGACTAAAGGAAAAAGCTAGTGATGATATACCAATGCCTACCTACTTTCGCTTCCTTGCCTTACTTGCCTTCAAGATATTTGCAGGAGAGAAGGTAGATGTTGCTATTTTAGAGGTTGGGTTAGGCGGGAAGTTTGACGCAACAAATGTGGTTCAGGCACCCGTCGTTTGTGGTATATCATCCCTTGGATATGATCACATGGAGATTCTTGGATATACTCTGGGAGCAATTGCTGGGGAGAAGGCTGGAATTTTCAAGCACGGGGTTCCTGCCTTCACTGTGCCTCAACCTGATGAAGCAATGCGGGTACTTGAAGAGAAAGCTTCTCAATTGGATGTACCTCTTCAAACAGTACCCCCAGTGGATGCTAATTTGCTGAATGGCTTAAAACTTGGACTTGAAGGTGAGCACCAATATGTTAATGCTGGCCTTGCTGTTGCACTGTGCTCTACATGGCTTCAAAGGACTGGTCATCTTGACATCACACACCCAGAAAATACTACCTCTCTGCCGGAGCAGTTTATAAAAGGACTAACGGCTACTGTCAGTTTACAAGGCCGAGCTCAAATTGTTCCAGATCACTATATTAACAATGAAAGCCCAGGAGATCTGACATTCTATTTAGATGGTGCCCATAGCCCCGAAAGCATGGAAATATGTGCAAGATGGTTTTCTCTTTCCACTAAAGAGAATTCTCAGGAAAAGACACTGGATTCCTCTAGATCACATGACTTCGTACAAAGGCATCCTGATGTGGAAGCCAAGAAGAACTCACAGATACTGCTGTTTAATTGTATGTCAGTGAGGGATCCTCAATTGCTTCTTCCGAACCTGATGAAAACATGTGCTAGTCATGGTGTCTACTTCAAGAAGGCGCTGTTTGTACCAAACACTTCAGTGTATCACAAGGTTGGTTCACATTCCTTACCTCCCACTGATCCTCAAGTTGATTTGTCGTGGCAATTCTCTCTTCAGAGAGTATGGGAAAATCTAATGGGAAATAAAGGAGGTGATGATAGGATCCCAGATACTCTTTCTGAAGAATTAAGAGATGATGTGGAAATGGCTGTCAAAAATTGTGAAAATAGTGCAGTTTTTTCCTCACTCCCATCAGCTATAAAGTGGCTCAGGGACAGTGTCCGACATAATCAATCTGCTCGTTTTCAGGTCCTTGTAACTGGTTCGCTACATCTTGTTGGTGATGTATTGAGATTAGTCAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

555

Amino Acids

61.64

Weight (kDa)

5.79

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07580 AT5G61590
fragaria_vesca FvH4_7g26940
malus_domestica MD01G1177200.v1.1 MD07G1248700.v1.1
prunus_persica Prupe.2G272500_v2.0.a1
pyrus_communis pycom07g22210
rosa_chinensis RchiOBHm_Chr1g0373641
rosa_laevigata RLG00000026810
rosa_multiflora Rmu_sc0013090.1_g000002
rosa_roxburghii Rroxscaffold_4G00284090
rosa_rugosa Rorug01G0378700
rosa_samantha Rh1AG388200 Rh1BG352500 Rh1CG365300
rosa_wichuraiana Rw1G034710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 965
Acc16I TGCGCA 1 cut(s) 240
Acc65I GGTACC 1 cut(s) 266
AccB1I GGYRCC 3 cut(s) 266, 598, 1070
AccI GTMKAC 1 cut(s) 1302
AccII CGCG 3 cut(s) 92, 159, 369
AciI CCGC 6 cut(s) 116, 157, 184, 328, 567, 745
AclWI GGATC 8 cut(s) 44, 276, 289, 1244, 1257, 1373, 1456, 1469
AcsI RAATTY 4 cut(s) 213, 410, 690, 1132
AcuI CTGAAG 3 cut(s) 1320, 1397, 1500
AfaI GTAC 6 cut(s) 268, 750, 780, 795, 1179, 1326
AflIII ACRYGT 2 cut(s) 90, 1283
AgsI TTSAA 8 cut(s) 80, 514, 697, 755, 788, 842, 902, 1309
AjnI CCWGG 1 cut(s) 1042
AleI CACNNNNGTG 1 cut(s) 1337
AluBI AGCT 4 cut(s) 458, 764, 1004, 1556
AluI AGCT 4 cut(s) 458, 764, 1004, 1556
Alw21I GWGCWC 3 cut(s) 852, 890, 1006
AlwI GGATC 8 cut(s) 44, 276, 289, 1244, 1257, 1373, 1456, 1469
AlwNI CAGNNNCTG 1 cut(s) 1222
AoxI GGCC 2 cut(s) 868, 997
ApeKI GCWGC 1 cut(s) 153
ApoI RAATTY 4 cut(s) 213, 410, 690, 1132
ArsI GACNNNNNNTTYG 4 cut(s) 182, 214, 895, 927
Asp700I GAANNNNTTC 1 cut(s) 375
Asp718I GGTACC 1 cut(s) 266
AspLEI GCGC 4 cut(s) 153, 161, 241, 1318
AspS9I GGNCC 1 cut(s) 1609
AsuHPI GGTGA 4 cut(s) 22, 857, 1463, 1652
AvaII GGWCC 1 cut(s) 1609
BaeGI GKGCMC 1 cut(s) 1075
BaeI ACNNNNGTAYC 2 cut(s) 1211, 1244
BamHI GGATCC 3 cut(s) 281, 1249, 1461
BanI GGYRCC 3 cut(s) 266, 598, 1070
BanII GRGCYC 1 cut(s) 1006
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 3 cut(s) 852, 890, 1006
BbvI GCAGC 1 cut(s) 165
BccI CCATC 5 cut(s) 77, 380, 1061, 1101, 1558
BceAI ACGGC 1 cut(s) 993
BciT130I CCWGG 1 cut(s) 1044
BclI TGATCA 2 cut(s) 141, 634
BfaI CTAG 3 cut(s) 459, 1161, 1290
BfoI RGCGCY 1 cut(s) 1319
BglII AGATCT 1 cut(s) 1048
BisI GCNGC 3 cut(s) 117, 154, 157
BlsI GCNGC 3 cut(s) 118, 155, 158
Bme1390I CCNGG 1 cut(s) 1044
Bme18I GGWCC 1 cut(s) 1609
BmgT120I GGNCC 1 cut(s) 1609
BmiI GGNNCC 8 cut(s) 231, 268, 283, 600, 708, 1072, 1251, 1463
BmrFI CCNGG 1 cut(s) 1044
BmrI ACTGGG 1 cut(s) 794
BmsI GCATC 2 cut(s) 796, 1195
BmuI ACTGGG 1 cut(s) 794
BpiI GAAGAC 1 cut(s) 87
BplI GAGNNNNNCTC 2 cut(s) 1237, 1269
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 1 cut(s) 1568
BpuEI CTTGAG 1 cut(s) 1368
BsaBI GATNNNNATC 1 cut(s) 248
BsaJI CCNNGG 3 cut(s) 72, 625, 1042
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bse1I ACTGG 6 cut(s) 126, 337, 800, 913, 1155, 1624
Bse3DI GCAATG 1 cut(s) 747
Bse8I GATNNNNATC 1 cut(s) 248
BseBI CCWGG 1 cut(s) 1044
BseDI CCNNGG 3 cut(s) 72, 625, 1042
BseGI GGATG 5 cut(s) 391, 620, 781, 811, 1186
BseJI GATNNNNATC 1 cut(s) 248
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 3 cut(s) 223, 1151, 1582
BseNI ACTGG 6 cut(s) 126, 337, 800, 913, 1155, 1624
BseRI GAGGAG 3 cut(s) 185, 242, 339
BseSI GKGCMC 1 cut(s) 1075
BseXI GCAGC 1 cut(s) 165
BseYI CCCAGC 1 cut(s) 674
BsgI GTGCAG 1 cut(s) 1551
Bsh1236I CGCG 3 cut(s) 92, 159, 369
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 2 cut(s) 870, 999
BshNI GGYRCC 3 cut(s) 266, 598, 1070
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 3 cut(s) 852, 890, 1006
BsiSI CCGG 1 cut(s) 953
BslFI GGGAC 1 cut(s) 1586
BsmFI GGGAC 1 cut(s) 1586
BsnI GGCC 2 cut(s) 870, 999
Bsp1286I GDGCHC 4 cut(s) 852, 890, 1006, 1075
Bsp19I CCATGG 1 cut(s) 72
Bsp68I TCGCGA 1 cut(s) 369
BspACI CCGC 6 cut(s) 116, 157, 184, 328, 567, 745
BspANI GGCC 2 cut(s) 870, 999
BspCNI CTCAG 3 cut(s) 222, 1150, 1581
BspFNI CGCG 3 cut(s) 92, 159, 369
BspLI GGNNCC 8 cut(s) 231, 268, 283, 600, 708, 1072, 1251, 1463
BspPI GGATC 8 cut(s) 44, 276, 289, 1244, 1257, 1373, 1456, 1469
BspT107I GGYRCC 3 cut(s) 266, 598, 1070
BsrDI GCAATG 1 cut(s) 747
BsrI ACTGG 6 cut(s) 126, 337, 800, 913, 1155, 1624
BssECI CCNNGG 3 cut(s) 72, 625, 1042
BssT1I CCWWGG 2 cut(s) 72, 625
Bst2UI CCWGG 1 cut(s) 1044
Bst4CI ACNGT 5 cut(s) 721, 793, 885, 985, 1577
Bst6I CTCTTC 4 cut(s) 399, 750, 789, 1416
BstC8I GCNNGC 1 cut(s) 868
BstDEI CTNAG 3 cut(s) 209, 1137, 1568
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 5 cut(s) 391, 620, 781, 811, 1186
BstFNI CGCG 3 cut(s) 92, 159, 369
BstH2I RGCGCY 1 cut(s) 1319
BstHHI GCGC 4 cut(s) 153, 161, 241, 1318
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 6 cut(s) 125, 177, 238, 506, 872, 1086
BstNI CCWGG 1 cut(s) 1044
BstNSI RCATGY 2 cut(s) 291, 1287
BstSCI CCNGG 1 cut(s) 1042
BstSLI GKGCMC 1 cut(s) 1075
BstUI CGCG 3 cut(s) 92, 159, 369
BstV1I GCAGC 1 cut(s) 165
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 5 cut(s) 49, 281, 1048, 1249, 1461
BstYI RGATCY 5 cut(s) 49, 281, 1048, 1249, 1461
BsuRI GGCC 2 cut(s) 870, 999
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 5 cut(s) 391, 620, 781, 811, 1186
BtuMI TCGCGA 1 cut(s) 369
Cac8I GCNNGC 1 cut(s) 868
CaiI CAGNNNCTG 1 cut(s) 1222
CfoI GCGC 4 cut(s) 153, 161, 241, 1318
Cfr13I GGNCC 1 cut(s) 1609
CseI GACGC 3 cut(s) 81, 98, 587
Csp6I GTAC 6 cut(s) 267, 749, 779, 794, 1178, 1325
CspCI CAANNNNNGTGG 2 cut(s) 570, 605
CviAII CATG 9 cut(s) 73, 257, 288, 640, 894, 1090, 1169, 1284, 1295
CviQI GTAC 6 cut(s) 267, 749, 779, 794, 1178, 1325
DdeI CTNAG 3 cut(s) 209, 1137, 1568
Eam1104I CTCTTC 4 cut(s) 399, 750, 789, 1416
EarI CTCTTC 4 cut(s) 399, 750, 789, 1416
Ecl136II GAGCTC 1 cut(s) 1004
Eco130I CCWWGG 2 cut(s) 72, 625
Eco24I GRGCYC 1 cut(s) 1006
Eco47I GGWCC 1 cut(s) 1609
Eco53kI GAGCTC 1 cut(s) 1004
Eco57I CTGAAG 3 cut(s) 1320, 1397, 1500
EcoICRI GAGCTC 1 cut(s) 1004
EcoO109I RGGNCCY 1 cut(s) 1609
EcoRI GAATTC 2 cut(s) 213, 1132
EcoRII CCWGG 1 cut(s) 1042
EcoT14I CCWWGG 2 cut(s) 72, 625
EcoT38I GRGCYC 1 cut(s) 1006
ErhI CCWWGG 2 cut(s) 72, 625
FaeI CATG 9 cut(s) 76, 260, 291, 643, 897, 1093, 1172, 1287, 1298
FaqI GGGAC 1 cut(s) 1586
FatI CATG 9 cut(s) 72, 256, 287, 639, 893, 1089, 1168, 1283, 1294
FauI CCCGC 2 cut(s) 560, 738
FbaI TGATCA 2 cut(s) 141, 634
FblI GTMKAC 1 cut(s) 1302
Fnu4HI GCNGC 3 cut(s) 117, 154, 157
FokI GGATG 5 cut(s) 398, 607, 788, 818, 1173
FriOI GRGCYC 1 cut(s) 1006
Fsp4HI GCNGC 3 cut(s) 117, 154, 157
FspBI CTAG 3 cut(s) 459, 1161, 1290
FspI TGCGCA 1 cut(s) 240
GlaI GCGC 4 cut(s) 152, 160, 240, 1317
GluI GCNGC 3 cut(s) 117, 154, 157
GsaI CCCAGC 1 cut(s) 678
GsuI CTGGAG 1 cut(s) 143
HaeII RGCGCY 1 cut(s) 1319
HaeIII GGCC 2 cut(s) 870, 999
HapII CCGG 1 cut(s) 953
HgaI GACGC 3 cut(s) 81, 98, 587
HhaI GCGC 4 cut(s) 153, 161, 241, 1318
Hin1II CATG 9 cut(s) 76, 260, 291, 643, 897, 1093, 1172, 1287, 1298
Hin6I GCGC 4 cut(s) 151, 159, 239, 1316
HinP1I GCGC 4 cut(s) 151, 159, 239, 1316
HindIII AAGCTT 1 cut(s) 762
HinfI GANTC 3 cut(s) 302, 646, 1154
HpaII CCGG 1 cut(s) 953
HphI GGTGA 4 cut(s) 22, 857, 1463, 1652
Hpy166II GTNNAC 4 cut(s) 394, 992, 1303, 1358
Hpy188I TCNGA 8 cut(s) 49, 212, 301, 1053, 1269, 1416, 1480, 1583
Hpy8I GTNNAC 4 cut(s) 394, 992, 1303, 1358
Hpy99I CGWCG 2 cut(s) 92, 608
HpyAV CCTTC 7 cut(s) 304, 520, 526, 676, 724, 836, 1306
HpyCH4III ACNGT 5 cut(s) 721, 793, 885, 985, 1577
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 5 cut(s) 291, 524, 881, 1103, 1532
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 177, 238, 506, 872, 1086
HpyF3I CTNAG 3 cut(s) 209, 1137, 1568
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 9 cut(s) 76, 260, 291, 643, 897, 1093, 1172, 1287, 1298
HspAI GCGC 4 cut(s) 151, 159, 239, 1316
KpnI GGTACC 1 cut(s) 270
Ksp22I TGATCA 2 cut(s) 141, 634
LmnI GCTCC 3 cut(s) 229, 665, 955
Lsp1109I GCAGC 1 cut(s) 165
LweI GCATC 2 cut(s) 796, 1195
MaeI CTAG 3 cut(s) 459, 1161, 1290
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 1 cut(s) 1615
MboII GAAGA 9 cut(s) 87, 259, 416, 767, 776, 1219, 1256, 1403, 1493
MfeI CAATTG 3 cut(s) 669, 770, 1256
MflI RGATCY 5 cut(s) 49, 281, 1048, 1249, 1461
MhlI GDGCHC 4 cut(s) 852, 890, 1006, 1075
MluI ACGCGT 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 363, 1606
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 6 cut(s) 438, 827, 861, 1029, 1230, 1487
MslI CAYNNNNRTG 4 cut(s) 257, 855, 1191, 1337
MspI CCGG 1 cut(s) 953
MspR9I CCNGG 1 cut(s) 1044
MunI CAATTG 3 cut(s) 669, 770, 1256
MvaI CCWGG 1 cut(s) 1044
MvnI CGCG 3 cut(s) 92, 159, 369
MwoI GCNNNNNNNGC 6 cut(s) 125, 177, 238, 506, 872, 1086
NcoI CCATGG 1 cut(s) 72
NlaIII CATG 9 cut(s) 76, 260, 291, 643, 897, 1093, 1172, 1287, 1298
NlaIV GGNNCC 8 cut(s) 231, 268, 283, 600, 708, 1072, 1251, 1463
NmeAIII GCCGAG 1 cut(s) 1025
NruI TCGCGA 1 cut(s) 369
NsbI TGCGCA 1 cut(s) 240
NspI RCATGY 2 cut(s) 291, 1287
OliI CACNNNNGTG 1 cut(s) 1337
PciI ACATGT 1 cut(s) 1283
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 3 cut(s) 302, 646, 1154
PflFI GACNNNGTC 1 cut(s) 1577
PkrI GCNGC 3 cut(s) 118, 155, 158
PpuMI RGGWCCY 1 cut(s) 1609
PscI ACATGT 1 cut(s) 1283
PsiI TTATAA 1 cut(s) 965
Psp124BI GAGCTC 1 cut(s) 1006
Psp5II RGGWCCY 1 cut(s) 1609
Psp6I CCWGG 1 cut(s) 1042
PspFI CCCAGC 1 cut(s) 674
PspGI CCWGG 1 cut(s) 1042
PspN4I GGNNCC 8 cut(s) 231, 268, 283, 600, 708, 1072, 1251, 1463
PspPI GGNCC 1 cut(s) 1609
PspPPI RGGWCCY 1 cut(s) 1609
PstNI CAGNNNCTG 1 cut(s) 1222
PsuI RGATCY 5 cut(s) 49, 281, 1048, 1249, 1461
PsyI GACNNNGTC 1 cut(s) 1577
RruI TCGCGA 1 cut(s) 369
RsaI GTAC 6 cut(s) 268, 750, 780, 795, 1179, 1326
RsaNI GTAC 6 cut(s) 267, 749, 779, 794, 1178, 1325
RseI CAYNNNNRTG 4 cut(s) 257, 855, 1191, 1337
SacI GAGCTC 1 cut(s) 1006
SaqAI TTAA 6 cut(s) 438, 827, 861, 1029, 1230, 1487
SatI GCNGC 3 cut(s) 117, 154, 157
Sau96I GGNCC 1 cut(s) 1609
ScrFI CCNGG 1 cut(s) 1044
SduI GDGCHC 4 cut(s) 852, 890, 1006, 1075
SfaNI GCATC 2 cut(s) 796, 1195
SinI GGWCC 1 cut(s) 1609
SmiMI CAYNNNNRTG 4 cut(s) 257, 855, 1191, 1337
SmlI CTYRAG 1 cut(s) 1383
SmoI CTYRAG 1 cut(s) 1383
SsiI CCGC 6 cut(s) 116, 157, 184, 328, 567, 745
SspMI CTAG 3 cut(s) 459, 1161, 1290
SstI GAGCTC 1 cut(s) 1006
StyD4I CCNGG 1 cut(s) 1042
StyI CCWWGG 2 cut(s) 72, 625
TaaI ACNGT 5 cut(s) 721, 793, 885, 985, 1577
TaiI ACGT 1 cut(s) 316
TaqI TCGA 1 cut(s) 189
TauI GCSGC 2 cut(s) 119, 159
TfiI GAWTC 3 cut(s) 302, 646, 1154
Tru1I TTAA 6 cut(s) 438, 827, 861, 1029, 1230, 1487
Tru9I TTAA 6 cut(s) 438, 827, 861, 1029, 1230, 1487
TseI GCWGC 1 cut(s) 153
TspDTI ATGAA 5 cut(s) 245, 260, 750, 1050, 1292
Tth111I GACNNNGTC 1 cut(s) 1577
VpaK11BI GGWCC 1 cut(s) 1609
XapI RAATTY 4 cut(s) 213, 410, 690, 1132
XbaI TCTAGA 1 cut(s) 1160
XceI RCATGY 2 cut(s) 291, 1287
XmiI GTMKAC 1 cut(s) 1302
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 3 cut(s) 459, 1161, 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.