pycom07g22210

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015285
Physical Location & Seq
Forward (+)
23636831 .. 23637752
922 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g22210.2

Sequence Viewer

Length: 825 bp
ATGCGTTTTTCAAACAAAAAAACAAGTGATATTTCCTATATAAATTTCCGCACTTTCTCACCCCAAAAATCATCAAATATTATTTCTAAAATTAGAAAAATATCATCCATATCCTTTCCATGCATGGCAATTAGCTTCCCCTTCTGCTCTCACTTCAACATGCCAGCACATGAAGAACAAGATGATTCAACGCTTGAGCTCATCCGCCAATACCTCTTAGGAGACTTCACCTTCACAGATTCCTTCATTTCTCATCTCAATTTCCAATTCCAACCAGTCAAGCCAGAGTACTCGTCCCTCTCTGAATCAGGGCCCAGCTCCCCCATTTCAAACCCTAATCACCACAGCCCTCACTTCTCAACTTCCGAAACGAAACCCGAAATCGTTAACTTACCATCCATGGAAGCAGAGCCCCTGAATTTGAGCTCACCAAAGAGAAAAAATAGTACTTCTCAAAATCCCGGTCCAAAAGATGAATTACAACAAGTTTCAGGCCCTGGTGATGCGTTAAGGCATTACAGGGGAGTCCGGCGGAGGCCGTGGGGAAAATTCGCGGCGGAGATACGTGATCCGGCTCGGAAAGGTACTCGGGTTTGGCTAGGGACATTTGACACTGATGTTGATGCTGCCAAGGCCTATGATTGTGCAGCATTTAAGCTGAGGGGTAGGAAAGCAATACTGAATTTTCCGTTGGAGGCCGGAGTGTCGGAACCGCCGGTGAACACGGCGCGGAAGAGGAGGAGATCGGTGAAGCAGGAGGAGGTAGCAGAGACTGAAGAATTGGAGCTTCAAAGTTGGGTGCTTTGGGAAGGAGAAGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

275

Amino Acids

30.95

Weight (kDa)

8.69

Isoelectric Point (pI)

66.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07580 AT5G61590
fragaria_vesca FvH4_7g26940
malus_domestica MD01G1177200.v1.1 MD07G1248700.v1.1
prunus_persica Prupe.2G272500_v2.0.a1
pyrus_communis pycom07g22210
rosa_chinensis RchiOBHm_Chr1g0373641
rosa_laevigata RLG00000026810
rosa_multiflora Rmu_sc0013090.1_g000002
rosa_roxburghii Rroxscaffold_4G00284090
rosa_rugosa Rorug01G0378700
rosa_samantha Rh1AG388200 Rh1BG352500 Rh1CG365300
rosa_wichuraiana Rw1G034710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 554, 730
AciI CCGC 7 cut(s) 49, 205, 532, 554, 557, 713, 730
AclWI GGATC 1 cut(s) 563
AcsI RAATTY 4 cut(s) 43, 418, 548, 682
AcuI CTGAAG 1 cut(s) 795
AfaI GTAC 3 cut(s) 290, 448, 586
AgsI TTSAA 5 cut(s) 12, 157, 189, 330, 791
AjnI CCWGG 1 cut(s) 496
AjuI GAANNNNNNNTTGG 2 cut(s) 674, 706
AluBI AGCT 6 cut(s) 135, 199, 318, 426, 658, 787
AluI AGCT 6 cut(s) 135, 199, 318, 426, 658, 787
Alw21I GWGCWC 2 cut(s) 201, 428
Alw26I GTCTC 2 cut(s) 216, 764
AlwI GGATC 1 cut(s) 563
AlwNI CAGNNNCTG 2 cut(s) 497, 773
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 5 cut(s) 311, 493, 536, 633, 696
ApaI GGGCCC 1 cut(s) 315
ApeKI GCWGC 2 cut(s) 626, 647
ApoI RAATTY 4 cut(s) 43, 418, 548, 682
ArsI GACNNNNNNTTYG 2 cut(s) 448, 480
AspLEI GCGC 1 cut(s) 730
AspS9I GGNCC 4 cut(s) 311, 312, 464, 494
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 7 cut(s) 51, 220, 332, 420, 512, 730, 760
AvaI CYCGRG 1 cut(s) 588
AvaII GGWCC 1 cut(s) 464
BaeGI GKGCMC 1 cut(s) 315
BaeI ACNNNNGTAYC 2 cut(s) 576, 609
BanII GRGCYC 4 cut(s) 201, 315, 414, 428
Bbv12I GWGCWC 2 cut(s) 201, 428
BbvCI CCTCAGC 1 cut(s) 659
BbvI GCAGC 2 cut(s) 613, 659
BccI CCATC 1 cut(s) 403
BceAI ACGGC 2 cut(s) 523, 741
BciT130I CCWGG 1 cut(s) 498
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 2 cut(s) 216, 764
BfaI CTAG 1 cut(s) 599
BisI GCNGC 3 cut(s) 555, 627, 648
BlsI GCNGC 3 cut(s) 556, 628, 649
BmcAI AGTACT 2 cut(s) 290, 448
Bme1390I CCNGG 2 cut(s) 462, 498
Bme18I GGWCC 1 cut(s) 464
BmeT110I CYCGRG 1 cut(s) 588
BmgT120I GGNCC 4 cut(s) 311, 312, 464, 494
BmiI GGNNCC 2 cut(s) 313, 711
BmrFI CCNGG 2 cut(s) 462, 498
BmsI GCATC 2 cut(s) 493, 613
Bpu10I CCTNAGC 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 566
BsaJI CCNNGG 4 cut(s) 399, 496, 539, 630
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 1 cut(s) 275
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 4 cut(s) 399, 496, 539, 630
BseGI GGATG 3 cut(s) 104, 201, 395
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 3 cut(s) 751, 754, 773
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 2 cut(s) 613, 659
BseYI CCCAGC 1 cut(s) 314
BsgI GTGCAG 1 cut(s) 666
Bsh1236I CGCG 2 cut(s) 554, 730
BshFI GGCC 5 cut(s) 313, 495, 538, 635, 698
BsiHKAI GWGCWC 2 cut(s) 201, 428
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 5 cut(s) 462, 529, 572, 699, 716
BslFI GGGAC 2 cut(s) 280, 616
BsmAI GTCTC 2 cut(s) 216, 764
BsmFI GGGAC 2 cut(s) 280, 616
BsnI GGCC 5 cut(s) 313, 495, 538, 635, 698
BsoBI CYCGRG 1 cut(s) 588
Bsp120I GGGCCC 1 cut(s) 311
Bsp1286I GDGCHC 4 cut(s) 201, 315, 414, 428
Bsp143I GATC 2 cut(s) 568, 743
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 7 cut(s) 49, 205, 532, 554, 557, 713, 730
BspANI GGCC 5 cut(s) 313, 495, 538, 635, 698
BspCNI CTCAG 1 cut(s) 651
BspFNI CGCG 2 cut(s) 554, 730
BspLI GGNNCC 2 cut(s) 313, 711
BspPI GGATC 1 cut(s) 563
BsrFI RCCGGY 1 cut(s) 715
BsrI ACTGG 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 4 cut(s) 399, 496, 539, 630
BssMI GATC 2 cut(s) 568, 743
BssT1I CCWWGG 2 cut(s) 399, 630
Bst2UI CCWGG 1 cut(s) 498
Bst6I CTCTTC 1 cut(s) 728
BstBAI YACGTR 1 cut(s) 566
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 2 cut(s) 217, 659
BstDSI CCRYGG 2 cut(s) 399, 539
BstF5I GGATG 3 cut(s) 104, 201, 395
BstFNI CGCG 2 cut(s) 554, 730
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 2 cut(s) 571, 746
BstMAI GTCTC 2 cut(s) 216, 764
BstMBI GATC 2 cut(s) 568, 743
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 460, 496
BstSLI GKGCMC 1 cut(s) 315
BstUI CGCG 2 cut(s) 554, 730
BstV1I GCAGC 2 cut(s) 613, 659
BsuRI GGCC 5 cut(s) 313, 495, 538, 635, 698
BtgI CCRYGG 2 cut(s) 399, 539
BtsCI GGATG 3 cut(s) 104, 201, 395
BtsIMutI CAGTG 1 cut(s) 612
Cac8I GCNNGC 1 cut(s) 165
CaiI CAGNNNCTG 2 cut(s) 497, 773
CfoI GCGC 1 cut(s) 730
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 4 cut(s) 311, 312, 464, 494
Csp6I GTAC 3 cut(s) 289, 447, 585
CviAII CATG 5 cut(s) 120, 124, 160, 170, 400
CviQI GTAC 3 cut(s) 289, 447, 585
DdeI CTNAG 2 cut(s) 217, 659
DpnI GATC 2 cut(s) 570, 745
DpnII GATC 2 cut(s) 568, 743
Eam1104I CTCTTC 1 cut(s) 728
EarI CTCTTC 1 cut(s) 728
EciI GGCGGA 3 cut(s) 194, 547, 572
Ecl136II GAGCTC 2 cut(s) 199, 426
Eco130I CCWWGG 2 cut(s) 399, 630
Eco147I AGGCCT 1 cut(s) 635
Eco24I GRGCYC 4 cut(s) 201, 315, 414, 428
Eco47I GGWCC 1 cut(s) 464
Eco53kI GAGCTC 2 cut(s) 199, 426
Eco57I CTGAAG 1 cut(s) 795
Eco88I CYCGRG 1 cut(s) 588
EcoICRI GAGCTC 2 cut(s) 199, 426
EcoO109I RGGNCCY 2 cut(s) 311, 494
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 2 cut(s) 399, 630
EcoT22I ATGCAT 1 cut(s) 125
EcoT38I GRGCYC 4 cut(s) 201, 315, 414, 428
ErhI CCWWGG 2 cut(s) 399, 630
FaeI CATG 5 cut(s) 123, 127, 163, 173, 403
FaiI YATR 9 cut(s) 39, 41, 110, 121, 125, 161, 171, 401, 639
FaqI GGGAC 2 cut(s) 280, 616
FatI CATG 5 cut(s) 119, 123, 159, 169, 399
Fnu4HI GCNGC 3 cut(s) 555, 627, 648
FokI GGATG 3 cut(s) 91, 188, 382
FriOI GRGCYC 4 cut(s) 201, 315, 414, 428
Fsp4HI GCNGC 3 cut(s) 555, 627, 648
FspBI CTAG 1 cut(s) 599
GlaI GCGC 1 cut(s) 729
GluI GCNGC 3 cut(s) 555, 627, 648
GsaI CCCAGC 1 cut(s) 318
HaeIII GGCC 5 cut(s) 313, 495, 538, 635, 698
HapII CCGG 5 cut(s) 462, 529, 572, 699, 716
HhaI GCGC 1 cut(s) 730
Hin1II CATG 5 cut(s) 123, 127, 163, 173, 403
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 4 cut(s) 185, 239, 305, 525
HpaI GTTAAC 1 cut(s) 388
HpaII CCGG 5 cut(s) 462, 529, 572, 699, 716
HphI GGTGA 7 cut(s) 51, 220, 332, 420, 512, 730, 760
Hpy166II GTNNAC 2 cut(s) 388, 721
Hpy188I TCNGA 4 cut(s) 304, 367, 579, 709
Hpy8I GTNNAC 2 cut(s) 388, 721
HpyAV CCTTC 5 cut(s) 151, 241, 253, 803, 809
HpyCH4IV ACGT 1 cut(s) 565
HpyCH4V TGCA 2 cut(s) 123, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 2 cut(s) 217, 659
HpySE526I ACGT 1 cut(s) 565
Hsp92II CATG 5 cut(s) 123, 127, 163, 173, 403
HspAI GCGC 1 cut(s) 728
KspAI GTTAAC 1 cut(s) 388
Kzo9I GATC 2 cut(s) 568, 743
LmnI GCTCC 2 cut(s) 323, 784
Lsp1109I GCAGC 2 cut(s) 613, 659
LweI GCATC 2 cut(s) 493, 613
MaeI CTAG 1 cut(s) 599
MaeII ACGT 1 cut(s) 565
MalI GATC 2 cut(s) 570, 745
MboI GATC 2 cut(s) 568, 743
MboII GAAGA 3 cut(s) 185, 745, 788
MhlI GDGCHC 4 cut(s) 201, 315, 414, 428
MlyI GAGTC 1 cut(s) 534
MmeI TCCRAC 3 cut(s) 295, 672, 687
Mph1103I ATGCAT 1 cut(s) 125
MseI TTAA 3 cut(s) 387, 509, 654
MspI CCGG 5 cut(s) 462, 529, 572, 699, 716
MspR9I CCNGG 2 cut(s) 462, 498
MvaI CCWGG 1 cut(s) 498
MvnI CGCG 2 cut(s) 554, 730
MwoI GCNNNNNNNGC 1 cut(s) 632
NciI CCSGG 1 cut(s) 462
NcoI CCATGG 1 cut(s) 399
NdeII GATC 2 cut(s) 568, 743
NlaIII CATG 5 cut(s) 123, 127, 163, 173, 403
NlaIV GGNNCC 2 cut(s) 313, 711
NsiI ATGCAT 1 cut(s) 125
NspI RCATGY 1 cut(s) 163
PceI AGGCCT 1 cut(s) 635
PfeI GAWTC 3 cut(s) 185, 239, 305
PkrI GCNGC 3 cut(s) 556, 628, 649
PleI GAGTC 1 cut(s) 533
PpsI GAGTC 1 cut(s) 533
Ppu21I YACGTR 1 cut(s) 566
Psp124BI GAGCTC 2 cut(s) 201, 428
Psp6I CCWGG 1 cut(s) 496
PspFI CCCAGC 1 cut(s) 314
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 2 cut(s) 313, 711
PspOMI GGGCCC 1 cut(s) 311
PspPI GGNCC 4 cut(s) 311, 312, 464, 494
PstNI CAGNNNCTG 2 cut(s) 497, 773
RsaI GTAC 3 cut(s) 290, 448, 586
RsaNI GTAC 3 cut(s) 289, 447, 585
SacI GAGCTC 2 cut(s) 201, 428
SaqAI TTAA 3 cut(s) 387, 509, 654
SatI GCNGC 3 cut(s) 555, 627, 648
Sau3AI GATC 2 cut(s) 568, 743
Sau96I GGNCC 4 cut(s) 311, 312, 464, 494
ScaI AGTACT 2 cut(s) 290, 448
SchI GAGTC 1 cut(s) 534
ScrFI CCNGG 2 cut(s) 462, 498
SduI GDGCHC 4 cut(s) 201, 315, 414, 428
SfaNI GCATC 2 cut(s) 493, 613
SgrAI CRCCGGYG 1 cut(s) 715
SinI GGWCC 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SseBI AGGCCT 1 cut(s) 635
SsiI CCGC 7 cut(s) 49, 205, 532, 554, 557, 713, 730
SspI AATATT 1 cut(s) 79
SspMI CTAG 1 cut(s) 599
SstI GAGCTC 2 cut(s) 201, 428
StuI AGGCCT 1 cut(s) 635
StyD4I CCNGG 2 cut(s) 460, 496
StyI CCWWGG 2 cut(s) 399, 630
TaiI ACGT 1 cut(s) 568
TatI WGTACW 2 cut(s) 288, 446
TauI GCSGC 1 cut(s) 557
TfiI GAWTC 3 cut(s) 185, 239, 305
Tru1I TTAA 3 cut(s) 387, 509, 654
Tru9I TTAA 3 cut(s) 387, 509, 654
TscAI CASTG 1 cut(s) 619
TseI GCWGC 2 cut(s) 626, 647
TspDTI ATGAA 3 cut(s) 186, 235, 489
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 1 cut(s) 619
VpaK11BI GGWCC 1 cut(s) 464
XapI RAATTY 4 cut(s) 43, 418, 548, 682
XceI RCATGY 1 cut(s) 163
XspI CTAG 1 cut(s) 599
ZrmI AGTACT 2 cut(s) 290, 448
Zsp2I ATGCAT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.