Rw1G034710

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015285
Physical Location & Seq
Reverse (-)
62630638 .. 62631759
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034710.1

Sequence Viewer

Length: 651 bp
ATGGCAGCAACCCAAGAACAAGATGAGTCAACTCTCGAACTCATACGCCAACATCTCTTAGGAGACTTCACCTTCACAGACAGCTTCATCACCAATCTCAACTACGAACAGAGCCCAGAAATCTCCTACTTCCAACCCATCAAATCCGAGTACTCCATATCCGAGTCCGAGTCCGCCTCCCCCGTTTCAAACTACCTCAACCCGAAACCCGAACCCATAACCGAAGAAGAACCCATGAGCTCCAAGAGCGAGCCCAAGAGAAAGTCGAGTTCTTCCAACTCCAGCTCATCGACTGGCGAGGCCGCCAGGCATTACAGGGGAGTCCGGAGAAGGCCGTGGGGCAAGTTTGCGGCGGAGATCCGTGACCCGGCCCGGAAGGGGAGCCGGGTCTGGCTGGGGACGTTTGACACTGATGTGGATGCTGCTAAGGCCTATGATTGTGCTGCATTCAAGCTCAGGGGAAGGAAAGCTATACTGAATTTTCCGCTGGAGGCCGGAGAGTCCGACCCGCCAAGTAATACGGGTCGGAAAAGGAGGAAGTCAGTGAAGCAAGAGGAGGAGGAGGAGGAGGAGGAGGGGGAGGCGCCGGCTCCGGAGGCGGAAGAGGTGGAGGTTCAAAGTTGGGTAGATTGGGATGGAGAAGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

216

Amino Acids

24.27

Weight (kDa)

4.72

Isoelectric Point (pI)

80.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 104 - 154 1.4e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07580 AT5G61590
fragaria_vesca FvH4_7g26940
malus_domestica MD01G1177200.v1.1 MD07G1248700.v1.1
prunus_persica Prupe.2G272500_v2.0.a1
pyrus_communis pycom07g22210
rosa_chinensis RchiOBHm_Chr1g0373641
rosa_laevigata RLG00000026810
rosa_multiflora Rmu_sc0013090.1_g000002
rosa_roxburghii Rroxscaffold_4G00284090
rosa_rugosa Rorug01G0378700
rosa_samantha Rh1AG388200 Rh1BG352500 Rh1CG365300
rosa_wichuraiana Rw1G034710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 583
AccIII TCCGGA 2 cut(s) 324, 592
AciI CCGC 7 cut(s) 174, 303, 350, 353, 485, 509, 599
AclWI GGATC 1 cut(s) 352
AcsI RAATTY 1 cut(s) 478
AcyI GRCGYC 1 cut(s) 584
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 367, 378
AgsI TTSAA 3 cut(s) 189, 451, 617
AjnI CCWGG 1 cut(s) 305
AleI CACNNNNGTG 1 cut(s) 413
AluBI AGCT 5 cut(s) 84, 240, 285, 454, 470
AluI AGCT 5 cut(s) 84, 240, 285, 454, 470
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 57
AlwI GGATC 1 cut(s) 352
Aor13HI TCCGGA 2 cut(s) 324, 592
AoxI GGCC 5 cut(s) 300, 332, 369, 429, 492
ApeKI GCWGC 3 cut(s) 5, 422, 443
ApoI RAATTY 1 cut(s) 478
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 1 cut(s) 370
AsuC2I CCSGG 3 cut(s) 368, 373, 386
AsuHPI GGTGA 2 cut(s) 61, 82
BanI GGYRCC 1 cut(s) 583
BanII GRGCYC 3 cut(s) 116, 242, 255
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 3 cut(s) 17, 409, 430
BccI CCATC 2 cut(s) 146, 629
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 1 cut(s) 307
BcnI CCSGG 3 cut(s) 368, 373, 386
BcoDI GTCTC 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 587
BisI GCNGC 5 cut(s) 6, 303, 351, 423, 444
BlsI GCNGC 5 cut(s) 7, 304, 352, 424, 445
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 4 cut(s) 307, 368, 373, 386
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 3 cut(s) 383, 585, 591
BmrFI CCNGG 4 cut(s) 307, 368, 373, 386
BmsI GCATC 2 cut(s) 409, 634
BplI GAGNNNNNCTC 2 cut(s) 161, 193
BpmI CTGGAG 2 cut(s) 265, 509
Bpu10I CCTNAGC 2 cut(s) 426, 455
BpuMI CCSGG 3 cut(s) 368, 373, 386
BsaHI GRCGYC 1 cut(s) 584
BsaJI CCNNGG 1 cut(s) 335
BsaWI WCCGGW 2 cut(s) 324, 592
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 378
Bse118I RCCGGY 1 cut(s) 586
Bse1I ACTGG 1 cut(s) 298
BseAI TCCGGA 2 cut(s) 324, 592
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 335
BseGI GGATG 2 cut(s) 424, 640
BseLI CCNNNNNNNGG 2 cut(s) 367, 378
BseMII CTCAG 1 cut(s) 469
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 7 cut(s) 569, 572, 575, 578, 581, 584, 587
BseXI GCAGC 3 cut(s) 17, 409, 430
BseYI CCCAGC 1 cut(s) 394
BshFI GGCC 5 cut(s) 302, 334, 371, 431, 494
BshNI GGYRCC 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 242
BsiSI CCGG 7 cut(s) 325, 368, 373, 385, 495, 587, 593
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 2 cut(s) 367, 378
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 412
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 5 cut(s) 302, 334, 371, 431, 494
Bsp1286I GDGCHC 3 cut(s) 116, 242, 255
Bsp13I TCCGGA 2 cut(s) 324, 592
Bsp143I GATC 1 cut(s) 357
BspACI CCGC 7 cut(s) 174, 303, 350, 353, 485, 509, 599
BspANI GGCC 5 cut(s) 302, 334, 371, 431, 494
BspCNI CTCAG 1 cut(s) 468
BspEI TCCGGA 2 cut(s) 324, 592
BspLI GGNNCC 3 cut(s) 383, 585, 591
BspPI GGATC 1 cut(s) 352
BspT107I GGYRCC 1 cut(s) 583
BsrFI RCCGGY 1 cut(s) 586
BsrI ACTGG 1 cut(s) 298
BssAI RCCGGY 1 cut(s) 586
BssECI CCNNGG 1 cut(s) 335
BssMI GATC 1 cut(s) 357
BssNI GRCGYC 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 307
Bst6I CTCTTC 1 cut(s) 597
BstACI GRCGYC 1 cut(s) 584
BstC8I GCNNGC 2 cut(s) 251, 588
BstDEI CTNAG 3 cut(s) 58, 426, 455
BstDSI CCRYGG 1 cut(s) 335
BstF5I GGATG 2 cut(s) 424, 640
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 1 cut(s) 586
BstKTI GATC 1 cut(s) 360
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 1 cut(s) 357
BstMWI GCNNNNNNNGC 3 cut(s) 246, 428, 596
BstNI CCWGG 1 cut(s) 307
BstSCI CCNGG 4 cut(s) 305, 366, 371, 384
BstV1I GCAGC 3 cut(s) 17, 409, 430
BstX2I RGATCY 1 cut(s) 357
BstYI RGATCY 1 cut(s) 357
BsuRI GGCC 5 cut(s) 302, 334, 371, 431, 494
BtgI CCRYGG 1 cut(s) 335
BtsCI GGATG 2 cut(s) 424, 640
BtsIMutI CAGTG 2 cut(s) 408, 549
Cac8I GCNNGC 2 cut(s) 251, 588
CfoI GCGC 1 cut(s) 586
Cfr10I RCCGGY 1 cut(s) 586
Cfr13I GGNCC 1 cut(s) 370
Csp6I GTAC 1 cut(s) 151
CviAII CATG 1 cut(s) 235
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 3 cut(s) 58, 426, 455
DinI GGCGCC 1 cut(s) 585
DpnI GATC 1 cut(s) 359
DpnII GATC 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
EciI GGCGGA 3 cut(s) 163, 368, 614
Ecl136II GAGCTC 1 cut(s) 240
Eco147I AGGCCT 1 cut(s) 431
Eco24I GRGCYC 3 cut(s) 116, 242, 255
Eco53kI GAGCTC 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 305
EcoT38I GRGCYC 3 cut(s) 116, 242, 255
EgeI GGCGCC 1 cut(s) 585
EheI GGCGCC 1 cut(s) 585
FaeI CATG 1 cut(s) 238
FaiI YATR 6 cut(s) 44, 158, 218, 236, 435, 473
FaqI GGGAC 1 cut(s) 412
FatI CATG 1 cut(s) 234
FauI CCCGC 1 cut(s) 516
Fnu4HI GCNGC 5 cut(s) 6, 303, 351, 423, 444
FokI GGATG 2 cut(s) 431, 647
FriOI GRGCYC 3 cut(s) 116, 242, 255
Fsp4HI GCNGC 5 cut(s) 6, 303, 351, 423, 444
GlaI GCGC 1 cut(s) 585
GluI GCNGC 5 cut(s) 6, 303, 351, 423, 444
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 2 cut(s) 265, 509
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 5 cut(s) 302, 334, 371, 431, 494
HapII CCGG 7 cut(s) 325, 368, 373, 385, 495, 587, 593
HhaI GCGC 1 cut(s) 586
Hin1I GRCGYC 1 cut(s) 584
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 5 cut(s) 26, 164, 170, 321, 500
HpaII CCGG 7 cut(s) 325, 368, 373, 385, 495, 587, 593
HphI GGTGA 2 cut(s) 61, 82
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 5 cut(s) 148, 163, 169, 505, 528
Hpy188III TCNNGA 3 cut(s) 35, 325, 593
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 4 cut(s) 82, 324, 370, 456
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 1 cut(s) 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 246, 428, 596
HpyF3I CTNAG 3 cut(s) 58, 426, 455
HpySE526I ACGT 1 cut(s) 401
Hsp92I GRCGYC 1 cut(s) 584
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 1 cut(s) 584
KasI GGCGCC 1 cut(s) 583
Kpn2I TCCGGA 2 cut(s) 324, 592
KroI GCCGGC 1 cut(s) 586
KroNI GCCGGC 1 cut(s) 588
Kzo9I GATC 1 cut(s) 357
LmnI GCTCC 3 cut(s) 245, 381, 595
Lsp1109I GCAGC 3 cut(s) 17, 409, 430
LweI GCATC 2 cut(s) 409, 634
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 1 cut(s) 362
MalI GATC 1 cut(s) 359
MboI GATC 1 cut(s) 357
MboII GAAGA 4 cut(s) 236, 239, 264, 614
MflI RGATCY 1 cut(s) 357
MhlI GDGCHC 3 cut(s) 116, 242, 255
MluCI AATT 1 cut(s) 478
Mly113I GGCGCC 1 cut(s) 584
MlyI GAGTC 5 cut(s) 35, 173, 179, 330, 509
MmeI TCCRAC 4 cut(s) 157, 300, 506, 528
MroI TCCGGA 2 cut(s) 324, 592
MroNI GCCGGC 1 cut(s) 586
MslI CAYNNNNRTG 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 487
MspI CCGG 7 cut(s) 325, 368, 373, 385, 495, 587, 593
MspR9I CCNGG 4 cut(s) 307, 368, 373, 386
Mva1269I GAATGC 1 cut(s) 446
MvaI CCWGG 1 cut(s) 307
MwoI GCNNNNNNNGC 3 cut(s) 246, 428, 596
NaeI GCCGGC 1 cut(s) 588
NarI GGCGCC 1 cut(s) 584
NciI CCSGG 3 cut(s) 368, 373, 386
NdeII GATC 1 cut(s) 357
NgoMIV GCCGGC 1 cut(s) 586
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 3 cut(s) 383, 585, 591
NmuCI GTSAC 1 cut(s) 362
OliI CACNNNNGTG 1 cut(s) 413
PceI AGGCCT 1 cut(s) 431
PctI GAATGC 1 cut(s) 446
PdiI GCCGGC 1 cut(s) 588
PkrI GCNGC 5 cut(s) 7, 304, 352, 424, 445
PleI GAGTC 5 cut(s) 34, 172, 178, 329, 508
PluTI GGCGCC 1 cut(s) 587
PpsI GAGTC 5 cut(s) 34, 172, 178, 329, 508
Psp124BI GAGCTC 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 305
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 3 cut(s) 383, 585, 591
PspPI GGNCC 1 cut(s) 370
PsuI RGATCY 1 cut(s) 357
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 1 cut(s) 242
SatI GCNGC 5 cut(s) 6, 303, 351, 423, 444
Sau3AI GATC 1 cut(s) 357
Sau96I GGNCC 1 cut(s) 370
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 5 cut(s) 35, 173, 179, 330, 509
ScrFI CCNGG 4 cut(s) 307, 368, 373, 386
SduI GDGCHC 3 cut(s) 116, 242, 255
SfaNI GCATC 2 cut(s) 409, 634
SfoI GGCGCC 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 413
Sse9I AATT 1 cut(s) 478
SseBI AGGCCT 1 cut(s) 431
SsiI CCGC 7 cut(s) 174, 303, 350, 353, 485, 509, 599
SspDI GGCGCC 1 cut(s) 583
SstI GAGCTC 1 cut(s) 242
StuI AGGCCT 1 cut(s) 431
StyD4I CCNGG 4 cut(s) 305, 366, 371, 384
TaiI ACGT 1 cut(s) 404
TaqI TCGA 3 cut(s) 36, 266, 290
TasI AATT 1 cut(s) 478
TatI WGTACW 1 cut(s) 150
TauI GCSGC 2 cut(s) 305, 353
TscAI CASTG 2 cut(s) 415, 549
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 3 cut(s) 5, 422, 443
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 1 cut(s) 76
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 2 cut(s) 415, 549
XapI RAATTY 1 cut(s) 478
ZrmI AGTACT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.