MD01G1191600.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006649
Physical Location & Seq
Forward (+)
29022334 .. 29023866
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1191600.v1.1.491

Sequence Viewer

Length: 648 bp
ATGAATGTCGGTATGTTCAATAACTTCAATACAGGAGGCCAGCGGAGAAAATCATCATTAGCCCTGACTTCGGATGGTGCGAAAAATCAAGATAAGTCAAATGACAATGATGAGAAAAAGAAGGTGATGCATAGAGAAATCGAAAAGAAGAGAAGGCAAGAAATGGCCAACCTTTATGGATCACTTCGATCTGTACTTCCTCTTGAATTTATTAAGGGAAAACGTTCGTTGGCGGATCATATGAACGAGGCAGTGAATTATATCAAACAGCTGCAAACGAGAATCAAAATATTTGGTGCCAAGAGAGGTGAGCTAAAGATGGGGTCAGCCTCGTCCAATTTGAGTGCTAGTACTCTTGGCCATGGGAGCAGTGGTAGCTCATCAACTGCAGCAAGTACTCTAGTTGCAGTCCACCCTTGCTTAGCTGGCGTTGAGATTGTAGTCAGTACCGGCCGATCTCCAGAGCAAGGTTTTCTCCTATCAAGAATGCTTAAAATACTGCTTGGACAAGGACTTAATGTAATCAGCTGTTCTTCTACCCAGGTTAACAGAAGACTTGTCTACACCATCCAATCTGAGGTTAGCGATCCAACATGCGTAGACTTTTCTGGGTTGCAGCAGATTCTGACCGAAGCCTGTCATCATTGA

Protein Analysis

216

Amino Acids

23.43

Weight (kDa)

9.66

Isoelectric Point (pI)

55.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 40 - 92 6.7e-12 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AccI GTMKAC 2 cut(s) 561, 600
AciI CCGC 2 cut(s) 43, 233
AclI AACGTT 1 cut(s) 223
AclWI GGATC 3 cut(s) 187, 243, 581
AcoI YGGCCR 3 cut(s) 165, 358, 451
AcsI RAATTY 1 cut(s) 206
AfaI GTAC 4 cut(s) 195, 352, 397, 448
AfiI CCNNNNNNNGG 3 cut(s) 70, 467, 577
AgsI TTSAA 3 cut(s) 19, 28, 206
AjnI CCWGG 1 cut(s) 540
AluBI AGCT 5 cut(s) 271, 313, 378, 425, 528
AluI AGCT 5 cut(s) 271, 313, 378, 425, 528
AlwI GGATC 3 cut(s) 187, 243, 581
AlwNI CAGNNNCTG 1 cut(s) 625
AoxI GGCC 4 cut(s) 37, 165, 358, 451
ApeKI GCWGC 3 cut(s) 271, 389, 616
ApoI RAATTY 1 cut(s) 206
Asp700I GAANNNNTTC 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 136, 320
BalI TGGCCA 2 cut(s) 167, 360
BanI GGYRCC 1 cut(s) 296
BbsI GAAGAC 1 cut(s) 559
BbvI GCAGC 3 cut(s) 258, 401, 628
BccI CCATC 3 cut(s) 68, 313, 575
BciT130I CCWGG 1 cut(s) 542
BfaI CTAG 2 cut(s) 348, 401
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 3 cut(s) 272, 390, 617
BlpI GCTNAGC 1 cut(s) 421
BlsI GCNGC 3 cut(s) 273, 391, 618
BmcAI AGTACT 2 cut(s) 352, 397
Bme1390I CCNGG 1 cut(s) 542
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 542
BmsI GCATC 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 559
BpmI CTGGAG 1 cut(s) 444
Bpu1102I GCTNAGC 1 cut(s) 421
BsaJI CCNNGG 2 cut(s) 361, 540
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 467, 577
Bse118I RCCGGY 1 cut(s) 449
BseBI CCWGG 1 cut(s) 542
BseDI CCNNGG 2 cut(s) 361, 540
BseGI GGATG 2 cut(s) 79, 567
BseLI CCNNNNNNNGG 3 cut(s) 70, 467, 577
BseMII CTCAG 1 cut(s) 567
BseX3I CGGCCG 1 cut(s) 451
BseXI GCAGC 3 cut(s) 258, 401, 628
Bsh1285I CGRYCG 1 cut(s) 454
BshFI GGCC 4 cut(s) 39, 167, 360, 453
BshNI GGYRCC 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 454
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 3 cut(s) 70, 467, 577
BsmI GAATGC 1 cut(s) 492
BsnI GGCC 4 cut(s) 39, 167, 360, 453
Bsp143I GATC 5 cut(s) 179, 188, 235, 455, 586
Bsp1720I GCTNAGC 1 cut(s) 421
Bsp19I CCATGG 1 cut(s) 361
BspACI CCGC 2 cut(s) 43, 233
BspANI GGCC 4 cut(s) 39, 167, 360, 453
BspCNI CTCAG 1 cut(s) 568
BspLI GGNNCC 1 cut(s) 298
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 3 cut(s) 187, 243, 581
BspT107I GGYRCC 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 449
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 361, 540
BssMI GATC 5 cut(s) 179, 188, 235, 455, 586
BssT1I CCWWGG 1 cut(s) 361
Bst2UI CCWGG 1 cut(s) 542
Bst6I CTCTTC 1 cut(s) 143
BstC8I GCNNGC 2 cut(s) 41, 427
BstDEI CTNAG 2 cut(s) 421, 576
BstDSI CCRYGG 1 cut(s) 361
BstF5I GGATG 2 cut(s) 79, 567
BstKTI GATC 5 cut(s) 182, 191, 238, 458, 589
BstMBI GATC 5 cut(s) 179, 188, 235, 455, 586
BstMCI CGRYCG 1 cut(s) 454
BstMWI GCNNNNNNNGC 3 cut(s) 366, 375, 426
BstNI CCWGG 1 cut(s) 542
BstNSI RCATGY 1 cut(s) 597
BstSCI CCNGG 1 cut(s) 540
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 3 cut(s) 258, 401, 628
BstV2I GAAGAC 1 cut(s) 559
BstZI CGGCCG 1 cut(s) 451
BsuRI GGCC 4 cut(s) 39, 167, 360, 453
BtgI CCRYGG 1 cut(s) 361
BtsCI GGATG 2 cut(s) 79, 567
BtsI GCAGTG 2 cut(s) 258, 376
BtsIMutI CAGTG 2 cut(s) 258, 376
Cac8I GCNNGC 2 cut(s) 41, 427
CaiI CAGNNNCTG 1 cut(s) 625
Cfr10I RCCGGY 1 cut(s) 449
Csp6I GTAC 4 cut(s) 194, 351, 396, 447
CviAII CATG 2 cut(s) 362, 594
CviQI GTAC 4 cut(s) 194, 351, 396, 447
DdeI CTNAG 2 cut(s) 421, 576
DpnI GATC 5 cut(s) 181, 190, 237, 457, 588
DpnII GATC 5 cut(s) 179, 188, 235, 455, 586
EaeI YGGCCR 3 cut(s) 165, 358, 451
EagI CGGCCG 1 cut(s) 451
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
EciI GGCGGA 1 cut(s) 248
EclXI CGGCCG 1 cut(s) 451
Eco130I CCWWGG 1 cut(s) 361
Eco52I CGGCCG 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 540
EcoT14I CCWWGG 1 cut(s) 361
EcoT22I ATGCAT 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 361
FaeI CATG 2 cut(s) 365, 597
FaiI YATR 8 cut(s) 14, 132, 177, 240, 242, 261, 363, 595
FatI CATG 2 cut(s) 361, 593
FauNDI CATATG 1 cut(s) 240
FblI GTMKAC 2 cut(s) 561, 600
Fnu4HI GCNGC 3 cut(s) 272, 390, 617
FokI GGATG 2 cut(s) 86, 554
Fsp4HI GCNGC 3 cut(s) 272, 390, 617
FspBI CTAG 2 cut(s) 348, 401
GluI GCNGC 3 cut(s) 272, 390, 617
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 4 cut(s) 39, 167, 360, 453
HapII CCGG 1 cut(s) 450
Hin1II CATG 2 cut(s) 365, 597
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 2 cut(s) 282, 622
HpaI GTTAAC 1 cut(s) 547
HpaII CCGG 1 cut(s) 450
HphI GGTGA 2 cut(s) 136, 320
Hpy166II GTNNAC 4 cut(s) 412, 547, 562, 601
Hpy188I TCNGA 3 cut(s) 73, 577, 627
Hpy188III TCNNGA 4 cut(s) 89, 203, 461, 483
Hpy8I GTNNAC 4 cut(s) 412, 547, 562, 601
HpyAV CCTTC 2 cut(s) 115, 147
HpyCH4IV ACGT 1 cut(s) 223
HpyCH4V TGCA 5 cut(s) 130, 274, 389, 407, 616
HpyF10VI GCNNNNNNNGC 3 cut(s) 366, 375, 426
HpyF3I CTNAG 2 cut(s) 421, 576
HpySE526I ACGT 1 cut(s) 223
Hsp92II CATG 2 cut(s) 365, 597
KspAI GTTAAC 1 cut(s) 547
Kzo9I GATC 5 cut(s) 179, 188, 235, 455, 586
LmnI GCTCC 1 cut(s) 366
LpnPI CCDG 9 cut(s) 18, 53, 77, 411, 463, 474, 527, 554, 594
Lsp1109I GCAGC 3 cut(s) 258, 401, 628
LweI GCATC 1 cut(s) 117
MaeI CTAG 2 cut(s) 348, 401
MaeII ACGT 1 cut(s) 223
MalI GATC 5 cut(s) 181, 190, 237, 457, 588
MboI GATC 5 cut(s) 179, 188, 235, 455, 586
MboII GAAGA 3 cut(s) 160, 525, 564
MlsI TGGCCA 2 cut(s) 167, 360
MluCI AATT 3 cut(s) 206, 256, 337
MluNI TGGCCA 2 cut(s) 167, 360
MmeI TCCRAC 1 cut(s) 614
MnlI CCTC 6 cut(s) 29, 210, 241, 299, 340, 571
Mox20I TGGCCA 2 cut(s) 167, 360
Mph1103I ATGCAT 1 cut(s) 132
MroXI GAANNNNTTC 1 cut(s) 223
MscI TGGCCA 2 cut(s) 167, 360
MseI TTAA 4 cut(s) 213, 492, 516, 546
Msp20I TGGCCA 2 cut(s) 167, 360
MspA1I CMGCKG 3 cut(s) 43, 271, 528
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 1 cut(s) 542
Mva1269I GAATGC 1 cut(s) 492
MvaI CCWGG 1 cut(s) 542
MwoI GCNNNNNNNGC 3 cut(s) 366, 375, 426
NcoI CCATGG 1 cut(s) 361
NdeI CATATG 1 cut(s) 240
NdeII GATC 5 cut(s) 179, 188, 235, 455, 586
NlaIII CATG 2 cut(s) 365, 597
NlaIV GGNNCC 1 cut(s) 298
NsiI ATGCAT 1 cut(s) 132
NspI RCATGY 1 cut(s) 597
PcsI WCGNNNNNNNCGW 1 cut(s) 77
PctI GAATGC 1 cut(s) 492
PdmI GAANNNNTTC 1 cut(s) 223
PfeI GAWTC 2 cut(s) 282, 622
PkrI GCNGC 3 cut(s) 273, 391, 618
Psp1406I AACGTT 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 540
PspGI CCWGG 1 cut(s) 540
PspN4I GGNNCC 1 cut(s) 298
PstI CTGCAG 1 cut(s) 391
PstNI CAGNNNCTG 1 cut(s) 625
PvuII CAGCTG 2 cut(s) 271, 528
RsaI GTAC 4 cut(s) 195, 352, 397, 448
RsaNI GTAC 4 cut(s) 194, 351, 396, 447
SaqAI TTAA 4 cut(s) 213, 492, 516, 546
SatI GCNGC 3 cut(s) 272, 390, 617
Sau3AI GATC 5 cut(s) 179, 188, 235, 455, 586
ScaI AGTACT 2 cut(s) 352, 397
ScrFI CCNGG 1 cut(s) 542
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 387
Sse9I AATT 3 cut(s) 206, 256, 337
SsiI CCGC 2 cut(s) 43, 233
SspI AATATT 1 cut(s) 291
SspMI CTAG 2 cut(s) 348, 401
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 1 cut(s) 361
TaiI ACGT 1 cut(s) 226
TaqI TCGA 2 cut(s) 141, 187
TaqII GACCGA 1 cut(s) 644
TasI AATT 3 cut(s) 206, 256, 337
TatI WGTACW 3 cut(s) 193, 350, 395
TfiI GAWTC 2 cut(s) 282, 622
Tru1I TTAA 4 cut(s) 213, 492, 516, 546
Tru9I TTAA 4 cut(s) 213, 492, 516, 546
TscAI CASTG 2 cut(s) 258, 376
TseI GCWGC 3 cut(s) 271, 389, 616
TspDTI ATGAA 2 cut(s) 17, 257
TspRI CASTG 2 cut(s) 258, 376
XapI RAATTY 1 cut(s) 206
XceI RCATGY 1 cut(s) 597
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XmiI GTMKAC 2 cut(s) 561, 600
XmnI GAANNNNTTC 1 cut(s) 223
XspI CTAG 2 cut(s) 348, 401
ZrmI AGTACT 2 cut(s) 352, 397
Zsp2I ATGCAT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.