MD01G1197200.v1.1

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016516
Physical Location & Seq
Forward (+)
29540127 .. 29541840
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1197200.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGGGACTGGTTTCTCCTTCTTTTCTAGGGATATTCCCAGTTCTTCTCCTCCAACAAAACCGTGTCTTGGGGACTTACCAGAGAGTTGTGTTTCATCCATTCTTATGTCCTTGGATCCGCTGGAGATTTGTAAATCGGCTCAGATTAACAAGGCTTTTCGAGCCGCTTCTTTGGCTGATTTTGTATGGGAGTCAAAGCTGCCTTCAAACTATAAGTTTCTCGTCGACAAAGTTCTTGATCTTGAAAGTTTGCCCAAGAAAGAGTTATACGCTAAGTTGTGCCAGCCCAATCGTTTTGATGGTGGCACCAAGGAAGCTTGGTTGGATAAGAGTTGTGGACAAGTTTTCTTGGCCATTTCATCAAAGGCTTTGCGGATAACAGGAATAGACGATCGAAGATATTGGAGTCATATCTCAACTGAGGAATCGAGGTTCCACACAGTTGCGTATCTTAAAAAATTTTGGTGGGTTGAAGCCCTAGGTGAACTGGAGTTTGAGTTTCCCCCAGGGAGCTACAACCTGTACTTCCGGCTTCAGCTAGGCAAAACTTGTACGAAAAGATTTGGCCGGCGAGTATGCACCGTTGATCAAGTCCACGGGTGGCACATCAAGCCGGTCCGATTCCAGTTATCGACCTCCGAGGGCCAGCACGCTGTATCCGAATGTTATTTGCATGAAGTAGGCAGCTGGGTTCATTACCATGTGGGTGACTTTGTTGTCAACAAGCCTAACATGCTAATCAAGATCAATTTTTCAATGGTCCAGATTGATTGTACACACACCAAAGGTGGTCTCTGCTTAGACTCTGTGTTAATAACCCCAAGCAAGTTCAGAGAAAAGTTAATAAAATGTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

286

Amino Acids

32.43

Weight (kDa)

8.69

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 117 - 274 2e-35 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016516)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52120
fragaria_vesca FvH4_7g29070 FvH4_7g29070
malus_domestica MD01G1197200.v1.1
prunus_persica Prupe.2G290600_v2.0.a1
pyrus_communis pycom01g20760
rosa_chinensis RchiOBHm_Chr1g0376791
rosa_roxburghii Rroxscaffold_4G00281730
rosa_rugosa Rorug01G0398400
rosa_samantha Rh1AG415600 Rh1BG375100 Rh1CG388900 Rh1DG405700
rosa_wichuraiana Rw1G036460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 716
AccB1I GGYRCC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 225
AciI CCGC 3 cut(s) 119, 165, 373
AclWI GGATC 2 cut(s) 110, 123
AcoI YGGCCR 2 cut(s) 351, 565
AcsI RAATTY 1 cut(s) 458
AcuI CTGAAG 1 cut(s) 518
AfaI GTAC 4 cut(s) 524, 553, 775, 853
AfiI CCNNNNNNNGG 1 cut(s) 68
AgsI TTSAA 4 cut(s) 207, 245, 473, 756
AjnI CCWGG 1 cut(s) 505
AloI GAACNNNNNNTCC 2 cut(s) 416, 448
AluBI AGCT 5 cut(s) 199, 317, 513, 538, 687
AluI AGCT 5 cut(s) 199, 317, 513, 538, 687
Alw26I GTCTC 1 cut(s) 797
AlwI GGATC 2 cut(s) 110, 123
AoxI GGCC 3 cut(s) 351, 565, 643
ApeKI GCWGC 2 cut(s) 199, 684
ApoI RAATTY 1 cut(s) 458
AspA2I CCTAGG 1 cut(s) 478
AspS9I GGNCC 3 cut(s) 616, 643, 760
AsuHPI GGTGA 2 cut(s) 494, 719
AvaII GGWCC 2 cut(s) 616, 760
AvrII CCTAGG 1 cut(s) 478
BalI TGGCCA 1 cut(s) 353
BamHI GGATCC 1 cut(s) 115
BanI GGYRCC 1 cut(s) 305
BbvI GCAGC 2 cut(s) 186, 696
BccI CCATC 1 cut(s) 293
BciT130I CCWGG 1 cut(s) 507
BciVI GTATCC 1 cut(s) 667
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 797
BfaI CTAG 3 cut(s) 27, 479, 539
BfuI GTATCC 1 cut(s) 667
BisI GCNGC 3 cut(s) 165, 200, 685
BlnI CCTAGG 1 cut(s) 478
BlsI GCNGC 3 cut(s) 166, 201, 686
Bme1390I CCNGG 1 cut(s) 507
Bme18I GGWCC 2 cut(s) 616, 760
BmgT120I GGNCC 3 cut(s) 616, 643, 760
BmiI GGNNCC 3 cut(s) 117, 307, 434
BmrFI CCNGG 1 cut(s) 507
BmrI ACTGGG 1 cut(s) 33
BmuI ACTGGG 1 cut(s) 33
BpmI CTGGAG 2 cut(s) 143, 509
BsaAI YACGTR 1 cut(s) 855
BsaI GGTCTC 1 cut(s) 797
BsaJI CCNNGG 7 cut(s) 111, 309, 478, 505, 506, 595, 639
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse118I RCCGGY 2 cut(s) 567, 613
Bse1I ACTGG 4 cut(s) 13, 39, 492, 625
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 7 cut(s) 111, 309, 478, 505, 506, 595, 639
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMII CTCAG 2 cut(s) 155, 411
BseNI ACTGG 4 cut(s) 13, 39, 492, 625
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 2 cut(s) 186, 696
BseYI CCCAGC 1 cut(s) 687
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 3 cut(s) 353, 567, 645
BshNI GGYRCC 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 394
BsiSI CCGG 3 cut(s) 529, 568, 614
BslFI GGGAC 2 cut(s) 19, 86
BslI CCNNNNNNNGG 1 cut(s) 68
BsmAI GTCTC 1 cut(s) 797
BsmFI GGGAC 2 cut(s) 19, 86
BsnI GGCC 3 cut(s) 353, 567, 645
Bso31I GGTCTC 1 cut(s) 797
Bsp1407I TGTACA 1 cut(s) 773
Bsp143I GATC 5 cut(s) 115, 238, 391, 586, 744
BspACI CCGC 3 cut(s) 119, 165, 373
BspANI GGCC 3 cut(s) 353, 567, 645
BspCNI CTCAG 2 cut(s) 154, 412
BspLI GGNNCC 3 cut(s) 117, 307, 434
BspPI GGATC 2 cut(s) 110, 123
BspT107I GGYRCC 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 797
BsrFI RCCGGY 2 cut(s) 567, 613
BsrGI TGTACA 1 cut(s) 773
BsrI ACTGG 4 cut(s) 13, 39, 492, 625
BssAI RCCGGY 2 cut(s) 567, 613
BssECI CCNNGG 7 cut(s) 111, 309, 478, 505, 506, 595, 639
BssMI GATC 5 cut(s) 115, 238, 391, 586, 744
BssT1I CCWWGG 3 cut(s) 111, 309, 478
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 3 cut(s) 63, 442, 583
BstAUI TGTACA 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 855
BstC8I GCNNGC 4 cut(s) 284, 569, 647, 651
BstDEI CTNAG 4 cut(s) 141, 273, 420, 799
BstDSI CCRYGG 1 cut(s) 595
BstF5I GGATG 1 cut(s) 95
BstKTI GATC 5 cut(s) 118, 241, 394, 589, 747
BstMAI GTCTC 1 cut(s) 797
BstMBI GATC 5 cut(s) 115, 238, 391, 586, 744
BstMCI CGRYCG 1 cut(s) 394
BstMWI GCNNNNNNNGC 4 cut(s) 161, 173, 610, 733
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 1 cut(s) 736
BstSCI CCNGG 1 cut(s) 505
BstSNI TACGTA 1 cut(s) 855
BstV1I GCAGC 2 cut(s) 186, 696
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 3 cut(s) 353, 567, 645
BtgI CCRYGG 1 cut(s) 595
BtsCI GGATG 1 cut(s) 95
Cac8I GCNNGC 4 cut(s) 284, 569, 647, 651
Cfr10I RCCGGY 2 cut(s) 567, 613
Cfr13I GGNCC 3 cut(s) 616, 643, 760
CpoI CGGWCCG 1 cut(s) 616
Csp6I GTAC 4 cut(s) 523, 552, 774, 852
CspI CGGWCCG 1 cut(s) 616
CviAII CATG 3 cut(s) 674, 701, 733
CviQI GTAC 4 cut(s) 523, 552, 774, 852
DdeI CTNAG 4 cut(s) 141, 273, 420, 799
DpnI GATC 5 cut(s) 117, 240, 393, 588, 746
DpnII GATC 5 cut(s) 115, 238, 391, 586, 744
DrdI GACNNNNNNGTC 1 cut(s) 716
DseDI GACNNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 2 cut(s) 351, 565
Eco105I TACGTA 1 cut(s) 855
Eco130I CCWWGG 3 cut(s) 111, 309, 478
Eco31I GGTCTC 1 cut(s) 797
Eco47I GGWCC 2 cut(s) 616, 760
Eco57I CTGAAG 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 505
EcoT14I CCWWGG 3 cut(s) 111, 309, 478
ErhI CCWWGG 3 cut(s) 111, 309, 478
FaeI CATG 3 cut(s) 677, 704, 736
FaiI YATR 9 cut(s) 107, 187, 213, 268, 411, 577, 675, 702, 734
FaqI GGGAC 2 cut(s) 19, 86
FatI CATG 3 cut(s) 673, 700, 732
FbaI TGATCA 1 cut(s) 586
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 165, 200, 685
FokI GGATG 1 cut(s) 82
Fsp4HI GCNGC 3 cut(s) 165, 200, 685
FspBI CTAG 3 cut(s) 27, 479, 539
GluI GCNGC 3 cut(s) 165, 200, 685
GsaI CCCAGC 1 cut(s) 691
GsuI CTGGAG 2 cut(s) 143, 509
HaeIII GGCC 3 cut(s) 353, 567, 645
HapII CCGG 3 cut(s) 529, 568, 614
Hin1II CATG 3 cut(s) 677, 704, 736
HincII GTYRAC 2 cut(s) 226, 721
HindII GTYRAC 2 cut(s) 226, 721
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 5 cut(s) 191, 406, 425, 621, 803
HpaII CCGG 3 cut(s) 529, 568, 614
HphI GGTGA 2 cut(s) 494, 719
Hpy166II GTNNAC 6 cut(s) 226, 338, 485, 595, 721, 776
Hpy188I TCNGA 5 cut(s) 144, 620, 640, 661, 833
Hpy188III TCNNGA 4 cut(s) 236, 242, 742, 763
Hpy8I GTNNAC 6 cut(s) 226, 338, 485, 595, 721, 776
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 2 cut(s) 28, 213
HpyCH4III ACNGT 3 cut(s) 63, 442, 583
HpyCH4IV ACGT 1 cut(s) 854
HpyCH4V TGCA 2 cut(s) 579, 673
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 173, 610, 733
HpyF3I CTNAG 4 cut(s) 141, 273, 420, 799
HpySE526I ACGT 1 cut(s) 854
Hsp92II CATG 3 cut(s) 677, 704, 736
KroI GCCGGC 1 cut(s) 567
KroNI GCCGGC 1 cut(s) 569
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 5 cut(s) 115, 238, 391, 586, 744
LmnI GCTCC 1 cut(s) 510
Lsp1109I GCAGC 2 cut(s) 186, 696
MaeI CTAG 3 cut(s) 27, 479, 539
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 1 cut(s) 707
MalI GATC 5 cut(s) 117, 240, 393, 588, 746
MboI GATC 5 cut(s) 115, 238, 391, 586, 744
MboII GAAGA 2 cut(s) 36, 408
MflI RGATCY 1 cut(s) 115
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 2 cut(s) 458, 748
MluNI TGGCCA 1 cut(s) 353
MlyI GAGTC 3 cut(s) 200, 415, 797
MmeI TCCRAC 2 cut(s) 77, 303
MnlI CCTC 5 cut(s) 60, 415, 423, 634, 646
Mox20I TGGCCA 1 cut(s) 353
MroNI GCCGGC 1 cut(s) 567
MscI TGGCCA 1 cut(s) 353
MseI TTAA 4 cut(s) 147, 453, 812, 842
MslI CAYNNNNRTG 3 cut(s) 104, 699, 705
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 2 cut(s) 121, 687
MspI CCGG 3 cut(s) 529, 568, 614
MspR9I CCNGG 1 cut(s) 507
MvaI CCWGG 1 cut(s) 507
MwoI GCNNNNNNNGC 4 cut(s) 161, 173, 610, 733
NaeI GCCGGC 1 cut(s) 569
NdeII GATC 5 cut(s) 115, 238, 391, 586, 744
NgoMIV GCCGGC 1 cut(s) 567
NlaIII CATG 3 cut(s) 677, 704, 736
NlaIV GGNNCC 3 cut(s) 117, 307, 434
NmuCI GTSAC 1 cut(s) 707
NspI RCATGY 1 cut(s) 736
PasI CCCWGGG 1 cut(s) 506
PcsI WCGNNNNNNNCGW 1 cut(s) 657
PdiI GCCGGC 1 cut(s) 569
PfeI GAWTC 2 cut(s) 425, 621
PkrI GCNGC 3 cut(s) 166, 201, 686
Ple19I CGATCG 1 cut(s) 394
PleI GAGTC 3 cut(s) 199, 414, 797
PpsI GAGTC 3 cut(s) 199, 414, 797
Ppu21I YACGTR 1 cut(s) 855
Psp6I CCWGG 1 cut(s) 505
PspFI CCCAGC 1 cut(s) 687
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 3 cut(s) 117, 307, 434
PspPI GGNCC 3 cut(s) 616, 643, 760
PsuI RGATCY 1 cut(s) 115
PvuI CGATCG 1 cut(s) 394
PvuII CAGCTG 1 cut(s) 687
RsaI GTAC 4 cut(s) 524, 553, 775, 853
RsaNI GTAC 4 cut(s) 523, 552, 774, 852
RseI CAYNNNNRTG 3 cut(s) 104, 699, 705
Rsr2I CGGWCCG 1 cut(s) 616
RsrII CGGWCCG 1 cut(s) 616
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 4 cut(s) 147, 453, 812, 842
SatI GCNGC 3 cut(s) 165, 200, 685
Sau3AI GATC 5 cut(s) 115, 238, 391, 586, 744
Sau96I GGNCC 3 cut(s) 616, 643, 760
SchI GAGTC 3 cut(s) 200, 415, 797
ScrFI CCNGG 1 cut(s) 507
SinI GGWCC 2 cut(s) 616, 760
SmiMI CAYNNNNRTG 3 cut(s) 104, 699, 705
SnaBI TACGTA 1 cut(s) 855
Sse9I AATT 2 cut(s) 458, 748
SsiI CCGC 3 cut(s) 119, 165, 373
SspMI CTAG 3 cut(s) 27, 479, 539
StyD4I CCNGG 1 cut(s) 505
StyI CCWWGG 3 cut(s) 111, 309, 478
TaaI ACNGT 3 cut(s) 63, 442, 583
TaiI ACGT 1 cut(s) 857
TaqI TCGA 5 cut(s) 160, 225, 394, 428, 632
TasI AATT 2 cut(s) 458, 748
TatI WGTACW 2 cut(s) 522, 773
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 2 cut(s) 425, 621
Tru1I TTAA 4 cut(s) 147, 453, 812, 842
Tru9I TTAA 4 cut(s) 147, 453, 812, 842
TseFI GTSAC 1 cut(s) 707
TseI GCWGC 2 cut(s) 199, 684
Tsp45I GTSAC 1 cut(s) 707
TspDTI ATGAA 4 cut(s) 84, 348, 683, 690
VpaK11BI GGWCC 2 cut(s) 616, 760
XapI RAATTY 1 cut(s) 458
XceI RCATGY 1 cut(s) 736
XmaJI CCTAGG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 3 cut(s) 27, 479, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.