Rw1G036460

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016516
Physical Location & Seq
Reverse (-)
64380869 .. 64382847
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G036460.1

Sequence Viewer

Length: 849 bp
ATGGGGACTGGTTCCTCTATGTCTTCAAAGGATACCAGTAGATCAGGTTCTCCCGATCATCCTCCGGATCAAACTAAGCCGGGTCTCAGAGACCTTCCTGAAAGTTGTATCTCTTACATTCTTTTGTATTTGGATCCGCCGGAGATCTGTAAACTTGCGCAGGTGAATAGGGTTTTCCGGGCTGCTTCATCTACTGATTTTGTCTGGCAATCAAAGCTCCCATCAAACTATAAGTTTCTAGTCGAAAAATCATTAATCCTCGGCGAACATAATAGTAACCGTATTCCGAAGAAAGATATATACGCAAGATTGTGTCTGCCAAATCGTTTTGACAATGGCACCAAGGAAGTTTGGTTGGACAAGAGTTGTGGATCACATCCACAGGTTTGTTTGTCCATTTCATCAAAGGCATTGCGGATAACAGGAATAGATGATCGAAGATATTGGAGTAATATTCCAACTGAGGAATCGAGGTTTAACAGAGTTGCATATCTTAAACAAATCTGGTGGCTTGAAGCACAAGGGGAACTAGAGTTCGAGTTTCCACCGGGAAACTATAGCCTATATTTTCGGCTTCAGCTTGGCAGGACTTGTCCAGGAAGATTCGGCCGACGAGTATGCAACACTGATCAAGTGCATGGCTGGGATATTAAGCCCGTCCGATTCCACTTATCAACGTCGGACGGCCAGCACGCTTTATCCGAGTGTTATTTGCATGAACGATGCAGCTGGGTTCCCAAGCCGATGAAGATCAAATTTTCAGTGACCCAAATTGATTGTACACACACCAAAGGTGGCCTCTGCTTAGACTCCGTGTTAATATGCCCAACCAAGTTTAGGAAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

282

Amino Acids

32.24

Weight (kDa)

9.04

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 30 - 71 5.3e-08 F-box-like
PP2 PF14299 129 - 276 1.6e-31 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016516)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52120
fragaria_vesca FvH4_7g29070 FvH4_7g29070
malus_domestica MD01G1197200.v1.1
prunus_persica Prupe.2G290600_v2.0.a1
pyrus_communis pycom01g20760
rosa_chinensis RchiOBHm_Chr1g0376791
rosa_roxburghii Rroxscaffold_4G00281730
rosa_rugosa Rorug01G0398400
rosa_samantha Rh1AG415600 Rh1BG375100 Rh1CG388900 Rh1DG405700
rosa_wichuraiana Rw1G036460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 151
Acc16I TGCGCA 1 cut(s) 159
Acc36I ACCTGC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 338
AccIII TCCGGA 1 cut(s) 64
AciI CCGC 2 cut(s) 137, 415
AclWI GGATC 4 cut(s) 75, 128, 141, 379
AcoI YGGCCR 2 cut(s) 607, 685
AcsI RAATTY 1 cut(s) 755
AcuI CTGAAG 1 cut(s) 560
AfaI GTAC 1 cut(s) 781
AfiI CCNNNNNNNGG 1 cut(s) 837
AgsI TTSAA 2 cut(s) 27, 515
AjnI CCWGG 1 cut(s) 595
AloI GAACNNNNNNTCC 2 cut(s) 518, 550
AluBI AGCT 3 cut(s) 217, 580, 729
AluI AGCT 3 cut(s) 217, 580, 729
Alw26I GTCTC 2 cut(s) 84, 89
AlwI GGATC 4 cut(s) 75, 128, 141, 379
Aor13HI TCCGGA 1 cut(s) 64
AoxI GGCC 3 cut(s) 607, 685, 796
ApeKI GCWGC 2 cut(s) 182, 726
ApoI RAATTY 1 cut(s) 755
AseI ATTAAT 1 cut(s) 254
AspLEI GCGC 1 cut(s) 160
AsuC2I CCSGG 3 cut(s) 81, 179, 549
AsuHPI GGTGA 1 cut(s) 175
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 338
BbsI GAAGAC 1 cut(s) 15
BbvI GCAGC 2 cut(s) 169, 738
BccI CCATC 1 cut(s) 229
BceAI ACGGC 1 cut(s) 700
BcgI CGANNNNNNTGC 2 cut(s) 600, 634
BciT130I CCWGG 1 cut(s) 597
BciVI GTATCC 1 cut(s) 25
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 3 cut(s) 81, 179, 549
BcoDI GTCTC 2 cut(s) 84, 89
BfaI CTAG 2 cut(s) 239, 530
BfmI CTRYAG 1 cut(s) 556
BfuAI ACCTGC 1 cut(s) 151
BfuI GTATCC 1 cut(s) 25
BglII AGATCT 1 cut(s) 144
BisI GCNGC 2 cut(s) 183, 727
BlsI GCNGC 2 cut(s) 184, 728
Bme1390I CCNGG 4 cut(s) 81, 179, 549, 597
BmiI GGNNCC 4 cut(s) 13, 135, 340, 735
BmrFI CCNGG 4 cut(s) 81, 179, 549, 597
BmsI GCATC 1 cut(s) 713
BpiI GAAGAC 1 cut(s) 15
BpuMI CCSGG 3 cut(s) 81, 179, 549
BsaBI GATNNNNATC 1 cut(s) 749
BsaI GGTCTC 2 cut(s) 84, 89
BsaJI CCNNGG 2 cut(s) 259, 342
BsaWI WCCGGW 1 cut(s) 64
Bsc4I CCNNNNNNNGG 1 cut(s) 837
Bse1I ACTGG 2 cut(s) 13, 36
Bse3DI GCAATG 1 cut(s) 410
Bse8I GATNNNNATC 1 cut(s) 749
BseAI TCCGGA 1 cut(s) 64
BseBI CCWGG 1 cut(s) 597
BseDI CCNNGG 2 cut(s) 259, 342
BseGI GGATG 2 cut(s) 58, 376
BseJI GATNNNNATC 1 cut(s) 749
BseLI CCNNNNNNNGG 1 cut(s) 837
BseMI GCAATG 1 cut(s) 410
BseMII CTCAG 2 cut(s) 100, 453
BseNI ACTGG 2 cut(s) 13, 36
BseX3I CGGCCG 1 cut(s) 607
BseXI GCAGC 2 cut(s) 169, 738
BseYI CCCAGC 2 cut(s) 642, 729
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 3 cut(s) 609, 687, 798
BshNI GGYRCC 1 cut(s) 338
BsiEI CGRYCG 1 cut(s) 610
BsiSI CCGG 5 cut(s) 65, 80, 140, 178, 548
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 837
BsmAI GTCTC 2 cut(s) 84, 89
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 3 cut(s) 609, 687, 798
Bso31I GGTCTC 2 cut(s) 84, 89
Bsp13I TCCGGA 1 cut(s) 64
Bsp1407I TGTACA 1 cut(s) 779
Bsp143I GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 750
BspACI CCGC 2 cut(s) 137, 415
BspANI GGCC 3 cut(s) 609, 687, 798
BspCNI CTCAG 2 cut(s) 99, 454
BspEI TCCGGA 1 cut(s) 64
BspLI GGNNCC 4 cut(s) 13, 135, 340, 735
BspMI ACCTGC 1 cut(s) 151
BspPI GGATC 4 cut(s) 75, 128, 141, 379
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 2 cut(s) 84, 89
BsrDI GCAATG 1 cut(s) 410
BsrGI TGTACA 1 cut(s) 779
BsrI ACTGG 2 cut(s) 13, 36
BssECI CCNNGG 2 cut(s) 259, 342
BssMI GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 750
BssT1I CCWWGG 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 597
Bst4CI ACNGT 1 cut(s) 281
BstAUI TGTACA 1 cut(s) 779
BstC8I GCNNGC 2 cut(s) 689, 693
BstDEI CTNAG 4 cut(s) 75, 86, 462, 805
BstF5I GGATG 2 cut(s) 58, 376
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 9 cut(s) 44, 58, 70, 136, 147, 374, 436, 631, 753
BstMAI GTCTC 2 cut(s) 84, 89
BstMBI GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 750
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNI CCWGG 1 cut(s) 597
BstSCI CCNGG 4 cut(s) 79, 177, 547, 595
BstSFI CTRYAG 1 cut(s) 556
BstV1I GCAGC 2 cut(s) 169, 738
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 2 cut(s) 133, 144
BstYI RGATCY 2 cut(s) 133, 144
BstZI CGGCCG 1 cut(s) 607
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 3 cut(s) 609, 687, 798
BtsCI GGATG 2 cut(s) 58, 376
BtsIMutI CAGTG 2 cut(s) 624, 768
BveI ACCTGC 1 cut(s) 151
Cac8I GCNNGC 2 cut(s) 689, 693
CfoI GCGC 1 cut(s) 160
Csp6I GTAC 1 cut(s) 780
CspCI CAANNNNNGTGG 4 cut(s) 349, 384, 488, 523
CviAII CATG 2 cut(s) 638, 716
CviQI GTAC 1 cut(s) 780
DdeI CTNAG 4 cut(s) 75, 86, 462, 805
DpnI GATC 9 cut(s) 43, 57, 69, 135, 146, 373, 435, 630, 752
DpnII GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 750
EaeI YGGCCR 2 cut(s) 607, 685
EagI CGGCCG 1 cut(s) 607
EciI GGCGGA 1 cut(s) 126
EclXI CGGCCG 1 cut(s) 607
Eco130I CCWWGG 1 cut(s) 342
Eco31I GGTCTC 2 cut(s) 84, 89
Eco52I CGGCCG 1 cut(s) 607
Eco57I CTGAAG 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 595
EcoT14I CCWWGG 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 2 cut(s) 641, 719
FaqI GGGAC 1 cut(s) 19
FatI CATG 2 cut(s) 637, 715
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 2 cut(s) 183, 727
FokI GGATG 2 cut(s) 45, 363
Fsp4HI GCNGC 2 cut(s) 183, 727
FspBI CTAG 2 cut(s) 239, 530
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 1 cut(s) 159
GluI GCNGC 2 cut(s) 183, 727
GsaI CCCAGC 2 cut(s) 646, 733
HaeIII GGCC 3 cut(s) 609, 687, 798
HapII CCGG 5 cut(s) 65, 80, 140, 178, 548
HhaI GCGC 1 cut(s) 160
Hin1II CATG 2 cut(s) 641, 719
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 4 cut(s) 467, 603, 663, 809
HpaII CCGG 5 cut(s) 65, 80, 140, 178, 548
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 2 cut(s) 152, 782
Hpy188I TCNGA 5 cut(s) 89, 288, 662, 682, 703
Hpy188III TCNNGA 3 cut(s) 53, 65, 98
Hpy8I GTNNAC 2 cut(s) 152, 782
Hpy99I CGWCG 2 cut(s) 615, 682
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 677
HpyCH4V TGCA 5 cut(s) 488, 621, 637, 715, 726
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 4 cut(s) 75, 86, 462, 805
HpySE526I ACGT 1 cut(s) 677
Hsp92II CATG 2 cut(s) 641, 719
HspAI GCGC 1 cut(s) 158
Kpn2I TCCGGA 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 750
LmnI GCTCC 1 cut(s) 222
Lsp1109I GCAGC 2 cut(s) 169, 738
LweI GCATC 1 cut(s) 713
MaeI CTAG 2 cut(s) 239, 530
MaeII ACGT 1 cut(s) 677
MaeIII GTNAC 2 cut(s) 275, 763
MalI GATC 9 cut(s) 43, 57, 69, 135, 146, 373, 435, 630, 752
MboI GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 750
MboII GAAGA 5 cut(s) 15, 301, 450, 612, 760
MflI RGATCY 2 cut(s) 133, 144
MluCI AATT 2 cut(s) 755, 771
MlyI GAGTC 1 cut(s) 803
MmeI TCCRAC 3 cut(s) 336, 482, 660
MnlI CCTC 6 cut(s) 25, 72, 269, 457, 465, 809
MroI TCCGGA 1 cut(s) 64
MseI TTAA 6 cut(s) 254, 477, 495, 651, 818, 847
MspA1I CMGCKG 1 cut(s) 729
MspI CCGG 5 cut(s) 65, 80, 140, 178, 548
MspR9I CCNGG 4 cut(s) 81, 179, 549, 597
MvaI CCWGG 1 cut(s) 597
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 3 cut(s) 81, 179, 549
NdeII GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 750
NlaIII CATG 2 cut(s) 641, 719
NlaIV GGNNCC 4 cut(s) 13, 135, 340, 735
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 763
NsbI TGCGCA 1 cut(s) 159
PaqCI CACCTGC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 699
PfeI GAWTC 3 cut(s) 467, 603, 663
PfoI TCCNGGA 1 cut(s) 595
PkrI GCNGC 2 cut(s) 184, 728
PleI GAGTC 1 cut(s) 803
PpsI GAGTC 1 cut(s) 803
PshBI ATTAAT 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 595
PspFI CCCAGC 2 cut(s) 642, 729
PspGI CCWGG 1 cut(s) 595
PspN4I GGNNCC 4 cut(s) 13, 135, 340, 735
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PsuI RGATCY 2 cut(s) 133, 144
PvuII CAGCTG 1 cut(s) 729
RsaI GTAC 1 cut(s) 781
RsaNI GTAC 1 cut(s) 780
SaqAI TTAA 6 cut(s) 254, 477, 495, 651, 818, 847
SatI GCNGC 2 cut(s) 183, 727
Sau3AI GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 750
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 4 cut(s) 81, 179, 549, 597
SfaNI GCATC 1 cut(s) 713
SfcI CTRYAG 1 cut(s) 556
Sse9I AATT 2 cut(s) 755, 771
SsiI CCGC 2 cut(s) 137, 415
SspI AATATT 1 cut(s) 454
SspMI CTAG 2 cut(s) 239, 530
StyD4I CCNGG 4 cut(s) 79, 177, 547, 595
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 1 cut(s) 680
TaqI TCGA 4 cut(s) 243, 436, 470, 537
TasI AATT 2 cut(s) 755, 771
TatI WGTACW 1 cut(s) 779
TfiI GAWTC 3 cut(s) 467, 603, 663
Tru1I TTAA 6 cut(s) 254, 477, 495, 651, 818, 847
Tru9I TTAA 6 cut(s) 254, 477, 495, 651, 818, 847
TscAI CASTG 2 cut(s) 631, 768
TseFI GTSAC 1 cut(s) 763
TseI GCWGC 2 cut(s) 182, 726
Tsp45I GTSAC 1 cut(s) 763
TspDTI ATGAA 4 cut(s) 177, 390, 732, 761
TspGWI ACGGA 1 cut(s) 802
TspRI CASTG 2 cut(s) 631, 768
VspI ATTAAT 1 cut(s) 254
XapI RAATTY 1 cut(s) 755
XspI CTAG 2 cut(s) 239, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.