Rroxscaffold_4G00281730

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016516
Physical Location & Seq
Forward (+)
3923098 .. 3925384
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281730.1

Sequence Viewer

Length: 882 bp
ATGGGGGCTGGTTCCTCTATGTCTTCAAAGGATACCAGTAGATCAGGTTCTCCCGATCATCCTCCGGATCAAACTAAGCCGGGTCTCGGAGACCTACCTGAAAGTTGTATCTCTTACATTCTTTTGTATTTGGATCCGCCGGAGATCTGTAAACTTGCGCAGGTGAATAGGGTTTTCCGGGCTGCTTCATCTACTGATTTTGTCTGGCAATCAAAGCTCCCATCAAACCATAAGTTTCTAGTTGAAAAATCATTAGTCCTCGGCGAACATAATAGTAACCGTATTCCGAAGAAAGATATATACGCAAGATTATGTCGGCCAAATCGTTTTGACAATGGTACCAAGGAAGTTTGGTTGGACAAGAGTTGTGGATCACATCCACAGGTTTGTTTGTCCATTTCATCAAAGGCATTGCGGATAACAGGAATAGATGATCGAAGATATTGGAGTAATATTGCAACCGAGGAATCGAGGTTTAACAGAGTTGCATATCTTAAACAAATCTGGTGGCTTGAAGCACAAGGGGAACTAGAGTTCGAGTTTCCACTGGGAAACTATAGCCTATATTTTCGGCTTCAGCTTGGCAGGACTTGTTCAGGAAGATTCGGCCGACGAGTATGCAACACTGATCAAGTGCATGGCTGGGACATTAAGCCCGTCCGATTCCACTTATCTACATCGGACGGCCAGCACGCTTTATCCGAGTGTTATTTGCATGAACAATGCAGCTGGGTTCACTACCATGTTGGTGATTTCGTCTGCAACAGTCCTAAGCCGATGAAGATCAAATTTTCAGTGACCCAAATTGATTGTACACACACCAAAGGTGGCCTCTGCTTAGACTCCGTGTTAATATGCCCAACCAAGTTTAGGAAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

293

Amino Acids

33.32

Weight (kDa)

8.83

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 30 - 71 4.4e-08 F-box-like
PP2 PF14299 129 - 287 2.1e-37 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016516)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52120
fragaria_vesca FvH4_7g29070 FvH4_7g29070
malus_domestica MD01G1197200.v1.1
prunus_persica Prupe.2G290600_v2.0.a1
pyrus_communis pycom01g20760
rosa_chinensis RchiOBHm_Chr1g0376791
rosa_roxburghii Rroxscaffold_4G00281730
rosa_rugosa Rorug01G0398400
rosa_samantha Rh1AG415600 Rh1BG375100 Rh1CG388900 Rh1DG405700
rosa_wichuraiana Rw1G036460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 151
Acc16I TGCGCA 1 cut(s) 159
Acc36I ACCTGC 1 cut(s) 151
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 338
AccIII TCCGGA 1 cut(s) 64
AciI CCGC 2 cut(s) 137, 415
AclWI GGATC 4 cut(s) 75, 128, 141, 379
AcoI YGGCCR 3 cut(s) 317, 607, 685
AcsI RAATTY 1 cut(s) 788
AcuI CTGAAG 1 cut(s) 560
AfaI GTAC 2 cut(s) 340, 814
AfiI CCNNNNNNNGG 2 cut(s) 86, 870
AgsI TTSAA 3 cut(s) 27, 245, 515
AloI GAACNNNNNNTCC 2 cut(s) 518, 550
AluBI AGCT 3 cut(s) 217, 580, 729
AluI AGCT 3 cut(s) 217, 580, 729
Alw26I GTCTC 2 cut(s) 84, 89
AlwI GGATC 4 cut(s) 75, 128, 141, 379
Aor13HI TCCGGA 1 cut(s) 64
AoxI GGCC 4 cut(s) 317, 607, 685, 829
ApeKI GCWGC 2 cut(s) 182, 726
ApoI RAATTY 1 cut(s) 788
Asp718I GGTACC 1 cut(s) 338
AspLEI GCGC 1 cut(s) 160
AsuC2I CCSGG 2 cut(s) 81, 179
AsuHPI GGTGA 2 cut(s) 175, 761
BaeI ACNNNNGTAYC 2 cut(s) 322, 355
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 338
BbsI GAAGAC 1 cut(s) 15
BbvI GCAGC 2 cut(s) 169, 738
BccI CCATC 1 cut(s) 229
BceAI ACGGC 1 cut(s) 700
BcgI CGANNNNNNTGC 2 cut(s) 600, 634
BciVI GTATCC 1 cut(s) 25
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 2 cut(s) 81, 179
BcoDI GTCTC 2 cut(s) 84, 89
BfaI CTAG 2 cut(s) 239, 530
BfmI CTRYAG 1 cut(s) 556
BfuAI ACCTGC 1 cut(s) 151
BfuI GTATCC 1 cut(s) 25
BglII AGATCT 1 cut(s) 144
BisI GCNGC 2 cut(s) 183, 727
BlsI GCNGC 2 cut(s) 184, 728
Bme1390I CCNGG 2 cut(s) 81, 179
BmiI GGNNCC 3 cut(s) 13, 135, 340
BmrFI CCNGG 2 cut(s) 81, 179
BmrI ACTGGG 1 cut(s) 557
BmuI ACTGGG 1 cut(s) 557
BpiI GAAGAC 1 cut(s) 15
Bpu10I CCTNAGC 1 cut(s) 771
BpuMI CCSGG 2 cut(s) 81, 179
BsaBI GATNNNNATC 1 cut(s) 782
BsaI GGTCTC 2 cut(s) 84, 89
BsaJI CCNNGG 3 cut(s) 259, 342, 462
BsaWI WCCGGW 1 cut(s) 64
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 870
Bse1I ACTGG 2 cut(s) 36, 552
Bse3DI GCAATG 1 cut(s) 410
Bse8I GATNNNNATC 1 cut(s) 782
BseAI TCCGGA 1 cut(s) 64
BseDI CCNNGG 3 cut(s) 259, 342, 462
BseGI GGATG 2 cut(s) 58, 376
BseJI GATNNNNATC 1 cut(s) 782
BseLI CCNNNNNNNGG 2 cut(s) 86, 870
BseMI GCAATG 1 cut(s) 410
BseNI ACTGG 2 cut(s) 36, 552
BseX3I CGGCCG 1 cut(s) 607
BseXI GCAGC 2 cut(s) 169, 738
BseYI CCCAGC 2 cut(s) 642, 729
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 4 cut(s) 319, 609, 687, 831
BshNI GGYRCC 1 cut(s) 338
BsiEI CGRYCG 1 cut(s) 610
BsiSI CCGG 4 cut(s) 65, 80, 140, 178
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 2 cut(s) 86, 870
BsmAI GTCTC 2 cut(s) 84, 89
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 4 cut(s) 319, 609, 687, 831
Bso31I GGTCTC 2 cut(s) 84, 89
Bsp13I TCCGGA 1 cut(s) 64
Bsp1407I TGTACA 1 cut(s) 812
Bsp143I GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 783
BspACI CCGC 2 cut(s) 137, 415
BspANI GGCC 4 cut(s) 319, 609, 687, 831
BspEI TCCGGA 1 cut(s) 64
BspLI GGNNCC 3 cut(s) 13, 135, 340
BspMI ACCTGC 1 cut(s) 151
BspPI GGATC 4 cut(s) 75, 128, 141, 379
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 2 cut(s) 84, 89
BsrDI GCAATG 1 cut(s) 410
BsrGI TGTACA 1 cut(s) 812
BsrI ACTGG 2 cut(s) 36, 552
BssECI CCNNGG 3 cut(s) 259, 342, 462
BssMI GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 783
BssT1I CCWWGG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 281, 767
BstAUI TGTACA 1 cut(s) 812
BstC8I GCNNGC 2 cut(s) 689, 693
BstDEI CTNAG 3 cut(s) 75, 771, 838
BstF5I GGATG 2 cut(s) 58, 376
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 9 cut(s) 44, 58, 70, 136, 147, 374, 436, 631, 786
BstMAI GTCTC 2 cut(s) 84, 89
BstMBI GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 783
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstSCI CCNGG 2 cut(s) 79, 177
BstSFI CTRYAG 1 cut(s) 556
BstV1I GCAGC 2 cut(s) 169, 738
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 2 cut(s) 133, 144
BstYI RGATCY 2 cut(s) 133, 144
BstZI CGGCCG 1 cut(s) 607
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 4 cut(s) 319, 609, 687, 831
BtsCI GGATG 2 cut(s) 58, 376
BtsIMutI CAGTG 3 cut(s) 545, 624, 801
BveI ACCTGC 1 cut(s) 151
Cac8I GCNNGC 2 cut(s) 689, 693
CfoI GCGC 1 cut(s) 160
Csp6I GTAC 2 cut(s) 339, 813
CspCI CAANNNNNGTGG 4 cut(s) 349, 384, 488, 523
CviAII CATG 3 cut(s) 638, 716, 743
CviQI GTAC 2 cut(s) 339, 813
DdeI CTNAG 3 cut(s) 75, 771, 838
DpnI GATC 9 cut(s) 43, 57, 69, 135, 146, 373, 435, 630, 785
DpnII GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 783
EaeI YGGCCR 3 cut(s) 317, 607, 685
EagI CGGCCG 1 cut(s) 607
EciI GGCGGA 1 cut(s) 126
EclXI CGGCCG 1 cut(s) 607
Eco130I CCWWGG 1 cut(s) 342
Eco31I GGTCTC 2 cut(s) 84, 89
Eco52I CGGCCG 1 cut(s) 607
Eco57I CTGAAG 1 cut(s) 560
EcoT14I CCWWGG 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 3 cut(s) 641, 719, 746
FaqI GGGAC 1 cut(s) 659
FatI CATG 3 cut(s) 637, 715, 742
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 2 cut(s) 183, 727
FokI GGATG 2 cut(s) 45, 363
Fsp4HI GCNGC 2 cut(s) 183, 727
FspBI CTAG 2 cut(s) 239, 530
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 1 cut(s) 159
GluI GCNGC 2 cut(s) 183, 727
GsaI CCCAGC 2 cut(s) 646, 733
HaeIII GGCC 4 cut(s) 319, 609, 687, 831
HapII CCGG 4 cut(s) 65, 80, 140, 178
HhaI GCGC 1 cut(s) 160
Hin1II CATG 3 cut(s) 641, 719, 746
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 4 cut(s) 467, 603, 663, 842
HpaII CCGG 4 cut(s) 65, 80, 140, 178
HphI GGTGA 2 cut(s) 175, 761
Hpy166II GTNNAC 3 cut(s) 152, 736, 815
Hpy188I TCNGA 5 cut(s) 89, 288, 662, 682, 703
Hpy188III TCNNGA 3 cut(s) 53, 65, 597
Hpy8I GTNNAC 3 cut(s) 152, 736, 815
Hpy99I CGWCG 1 cut(s) 615
HpyCH4III ACNGT 2 cut(s) 281, 767
HpyCH4V TGCA 7 cut(s) 458, 488, 621, 637, 715, 726, 762
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 3 cut(s) 75, 771, 838
Hsp92II CATG 3 cut(s) 641, 719, 746
HspAI GCGC 1 cut(s) 158
Kpn2I TCCGGA 1 cut(s) 64
KpnI GGTACC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 783
LmnI GCTCC 1 cut(s) 222
Lsp1109I GCAGC 2 cut(s) 169, 738
MaeI CTAG 2 cut(s) 239, 530
MaeIII GTNAC 2 cut(s) 275, 796
MalI GATC 9 cut(s) 43, 57, 69, 135, 146, 373, 435, 630, 785
MboI GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 783
MboII GAAGA 5 cut(s) 15, 301, 450, 612, 793
MflI RGATCY 2 cut(s) 133, 144
MluCI AATT 2 cut(s) 788, 804
MlyI GAGTC 1 cut(s) 836
MmeI TCCRAC 1 cut(s) 336
MnlI CCTC 6 cut(s) 25, 72, 269, 457, 465, 842
MroI TCCGGA 1 cut(s) 64
MseI TTAA 5 cut(s) 477, 495, 651, 851, 880
MslI CAYNNNNRTG 2 cut(s) 741, 747
MspA1I CMGCKG 1 cut(s) 729
MspI CCGG 4 cut(s) 65, 80, 140, 178
MspR9I CCNGG 2 cut(s) 81, 179
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 2 cut(s) 81, 179
NdeII GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 783
NlaIII CATG 3 cut(s) 641, 719, 746
NlaIV GGNNCC 3 cut(s) 13, 135, 340
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 796
NsbI TGCGCA 1 cut(s) 159
PaqCI CACCTGC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 2 cut(s) 322, 699
PfeI GAWTC 3 cut(s) 467, 603, 663
PkrI GCNGC 2 cut(s) 184, 728
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
PspFI CCCAGC 2 cut(s) 642, 729
PspN4I GGNNCC 3 cut(s) 13, 135, 340
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PsuI RGATCY 2 cut(s) 133, 144
PvuII CAGCTG 1 cut(s) 729
RsaI GTAC 2 cut(s) 340, 814
RsaNI GTAC 2 cut(s) 339, 813
RseI CAYNNNNRTG 2 cut(s) 741, 747
SaqAI TTAA 5 cut(s) 477, 495, 651, 851, 880
SatI GCNGC 2 cut(s) 183, 727
Sau3AI GATC 9 cut(s) 41, 55, 67, 133, 144, 371, 433, 628, 783
SchI GAGTC 1 cut(s) 836
ScrFI CCNGG 2 cut(s) 81, 179
SfcI CTRYAG 1 cut(s) 556
SmiMI CAYNNNNRTG 2 cut(s) 741, 747
Sse9I AATT 2 cut(s) 788, 804
SsiI CCGC 2 cut(s) 137, 415
SspI AATATT 1 cut(s) 454
SspMI CTAG 2 cut(s) 239, 530
StyD4I CCNGG 2 cut(s) 79, 177
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 2 cut(s) 281, 767
TaqI TCGA 3 cut(s) 436, 470, 537
TasI AATT 2 cut(s) 788, 804
TatI WGTACW 1 cut(s) 812
TfiI GAWTC 3 cut(s) 467, 603, 663
Tru1I TTAA 5 cut(s) 477, 495, 651, 851, 880
Tru9I TTAA 5 cut(s) 477, 495, 651, 851, 880
TscAI CASTG 3 cut(s) 552, 631, 801
TseFI GTSAC 1 cut(s) 796
TseI GCWGC 2 cut(s) 182, 726
Tsp45I GTSAC 1 cut(s) 796
TspDTI ATGAA 4 cut(s) 177, 390, 732, 794
TspGWI ACGGA 1 cut(s) 835
TspRI CASTG 3 cut(s) 552, 631, 801
XapI RAATTY 1 cut(s) 788
XspI CTAG 2 cut(s) 239, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.