Rh1DG405700

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0016516
Physical Location & Seq
Reverse (-)
62490815 .. 62491579
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG405700.1

Sequence Viewer

Length: 528 bp
ATGGGGGCTGGTTCCTCTATGTCTTCAAAGGATACCAGTAGATCAGGTTCTCCCGATCATCCTCCTGATCAAACTAAGCCGGGTCTCGGAGACCTACCTGAAAGTTGTATCTCTTACATTCTTTTGTATTTGGATCCGCCGGAGATCTGTAAACTTGCGCAGGTGAATAGGGTTTTCCGGGCTGCTTCATCTACTGATTTTGTCTGGCAATCAAAGCTCCCATCAAACTATAAGTTTCTAGTCGAAAAATCATTAATCCTCGGCGAACATAATAGTAACCGTATTCTGAAGAAAGATATATACGCAAGATTGTGTCTGCCAAATCGTTTTGACAATGGCACCAAGGAAGTTTGGTTGGACAAGAGTTGTGGATCACATCCACAGGTTTGTTTGTCCATTTCATCAAAGGCATTGCGGATAACAGGAATAGATGATCGAAGATATTGGAGTAATATTCCAACTGAGGAATCGAGATTATCAGACCTCTACATTTATGAAGTGGATAGTTCATGCACCAAGCCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

175

Amino Acids

19.63

Weight (kDa)

7.58

Isoelectric Point (pI)

57.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 30 - 71 1.9e-08 F-box-like
F-box PF00646 31 - 69 5e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016516)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52120
fragaria_vesca FvH4_7g29070 FvH4_7g29070
malus_domestica MD01G1197200.v1.1
prunus_persica Prupe.2G290600_v2.0.a1
pyrus_communis pycom01g20760
rosa_chinensis RchiOBHm_Chr1g0376791
rosa_roxburghii Rroxscaffold_4G00281730
rosa_rugosa Rorug01G0398400
rosa_samantha Rh1AG415600 Rh1BG375100 Rh1CG388900 Rh1DG405700
rosa_wichuraiana Rw1G036460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 151
Acc16I TGCGCA 1 cut(s) 159
Acc36I ACCTGC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 338
AciI CCGC 2 cut(s) 137, 415
AclWI GGATC 3 cut(s) 128, 141, 379
AcuI CTGAAG 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 1 cut(s) 27
AluBI AGCT 1 cut(s) 217
AluI AGCT 1 cut(s) 217
Alw26I GTCTC 2 cut(s) 84, 89
AlwI GGATC 3 cut(s) 128, 141, 379
ApeKI GCWGC 1 cut(s) 182
AseI ATTAAT 1 cut(s) 254
AspLEI GCGC 1 cut(s) 160
AsuC2I CCSGG 2 cut(s) 81, 179
AsuHPI GGTGA 1 cut(s) 175
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 338
BbsI GAAGAC 1 cut(s) 15
BbvI GCAGC 1 cut(s) 169
BccI CCATC 1 cut(s) 229
BciVI GTATCC 1 cut(s) 25
BclI TGATCA 1 cut(s) 67
BcnI CCSGG 2 cut(s) 81, 179
BcoDI GTCTC 2 cut(s) 84, 89
BfaI CTAG 1 cut(s) 239
BfuAI ACCTGC 1 cut(s) 151
BfuI GTATCC 1 cut(s) 25
BglII AGATCT 1 cut(s) 144
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
Bme1390I CCNGG 2 cut(s) 81, 179
BmiI GGNNCC 3 cut(s) 13, 135, 340
BmrFI CCNGG 2 cut(s) 81, 179
BpiI GAAGAC 1 cut(s) 15
BpuMI CCSGG 2 cut(s) 81, 179
BsaI GGTCTC 2 cut(s) 84, 89
BsaJI CCNNGG 2 cut(s) 259, 342
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 36
Bse3DI GCAATG 1 cut(s) 410
BseDI CCNNGG 2 cut(s) 259, 342
BseGI GGATG 2 cut(s) 58, 376
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 1 cut(s) 410
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 169
BshNI GGYRCC 1 cut(s) 338
BsiSI CCGG 3 cut(s) 80, 140, 178
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 2 cut(s) 84, 89
Bso31I GGTCTC 2 cut(s) 84, 89
Bsp143I GATC 7 cut(s) 41, 55, 67, 133, 144, 371, 433
BspACI CCGC 2 cut(s) 137, 415
BspCNI CTCAG 1 cut(s) 454
BspLI GGNNCC 3 cut(s) 13, 135, 340
BspMI ACCTGC 1 cut(s) 151
BspPI GGATC 3 cut(s) 128, 141, 379
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 2 cut(s) 84, 89
BsrDI GCAATG 1 cut(s) 410
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 259, 342
BssMI GATC 7 cut(s) 41, 55, 67, 133, 144, 371, 433
BssT1I CCWWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 281
BstDEI CTNAG 2 cut(s) 75, 462
BstF5I GGATG 2 cut(s) 58, 376
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 7 cut(s) 44, 58, 70, 136, 147, 374, 436
BstMAI GTCTC 2 cut(s) 84, 89
BstMBI GATC 7 cut(s) 41, 55, 67, 133, 144, 371, 433
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstSCI CCNGG 2 cut(s) 79, 177
BstV1I GCAGC 1 cut(s) 169
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 2 cut(s) 133, 144
BstYI RGATCY 2 cut(s) 133, 144
BsuI GTATCC 1 cut(s) 25
BtsCI GGATG 2 cut(s) 58, 376
BveI ACCTGC 1 cut(s) 151
CfoI GCGC 1 cut(s) 160
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 1 cut(s) 510
CviJI RGCY 5 cut(s) 8, 79, 182, 217, 520
CviKI_1 RGCY 5 cut(s) 8, 79, 182, 217, 520
DdeI CTNAG 2 cut(s) 75, 462
DpnI GATC 7 cut(s) 43, 57, 69, 135, 146, 373, 435
DpnII GATC 7 cut(s) 41, 55, 67, 133, 144, 371, 433
EciI GGCGGA 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 342
Eco31I GGTCTC 2 cut(s) 84, 89
Eco57I CTGAAG 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 1 cut(s) 513
FaiI YATR 7 cut(s) 20, 231, 270, 299, 301, 495, 511
FatI CATG 1 cut(s) 509
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 1 cut(s) 183
FokI GGATG 2 cut(s) 45, 363
Fsp4HI GCNGC 1 cut(s) 183
FspBI CTAG 1 cut(s) 239
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 1 cut(s) 159
GluI GCNGC 1 cut(s) 183
HapII CCGG 3 cut(s) 80, 140, 178
HhaI GCGC 1 cut(s) 160
Hin1II CATG 1 cut(s) 513
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 1 cut(s) 467
HpaII CCGG 3 cut(s) 80, 140, 178
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 152
Hpy188I TCNGA 3 cut(s) 89, 288, 481
Hpy188III TCNNGA 3 cut(s) 53, 65, 471
Hpy8I GTNNAC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4V TGCA 1 cut(s) 513
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 2 cut(s) 75, 462
Hsp92II CATG 1 cut(s) 513
HspAI GCGC 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 7 cut(s) 41, 55, 67, 133, 144, 371, 433
LmnI GCTCC 1 cut(s) 222
Lsp1109I GCAGC 1 cut(s) 169
MaeI CTAG 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 275
MalI GATC 7 cut(s) 43, 57, 69, 135, 146, 373, 435
MboI GATC 7 cut(s) 41, 55, 67, 133, 144, 371, 433
MboII GAAGA 3 cut(s) 15, 301, 450
MflI RGATCY 2 cut(s) 133, 144
MmeI TCCRAC 2 cut(s) 336, 482
MnlI CCTC 5 cut(s) 25, 72, 269, 457, 494
MseI TTAA 1 cut(s) 254
MspI CCGG 3 cut(s) 80, 140, 178
MspR9I CCNGG 2 cut(s) 81, 179
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 2 cut(s) 81, 179
NdeII GATC 7 cut(s) 41, 55, 67, 133, 144, 371, 433
NlaIII CATG 1 cut(s) 513
NlaIV GGNNCC 3 cut(s) 13, 135, 340
NmeAIII GCCGAG 1 cut(s) 240
NsbI TGCGCA 1 cut(s) 159
PaqCI CACCTGC 1 cut(s) 151
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 1 cut(s) 184
PshBI ATTAAT 1 cut(s) 254
PspN4I GGNNCC 3 cut(s) 13, 135, 340
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PsuI RGATCY 2 cut(s) 133, 144
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 7 cut(s) 41, 55, 67, 133, 144, 371, 433
ScrFI CCNGG 2 cut(s) 81, 179
SetI ASST 7 cut(s) 49, 96, 100, 165, 219, 387, 486
SsiI CCGC 2 cut(s) 137, 415
SspI AATATT 1 cut(s) 454
SspMI CTAG 1 cut(s) 239
StyD4I CCNGG 2 cut(s) 79, 177
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 281
TaqI TCGA 3 cut(s) 243, 436, 470
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TseI GCWGC 1 cut(s) 182
TspDTI ATGAA 4 cut(s) 177, 390, 498, 510
VspI ATTAAT 1 cut(s) 254
XspI CTAG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.