MD01G1199200.v1.1
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010068
Physical Location & Seq
Forward (+)
29651650 .. 29654628
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1199200.v1.1.491

Sequence Viewer

Length: 609 bp
ATGTTTTCGTTGTTTTACGGACTATGGAAATATATGTTCAGTAAGACGGAGTTTCATGTGCTCATTCTTGGAATCGACAAGGCTGGGAAGACGACTTTGCTTGAGAAGTTGAAGTCAATGTACTCAAACTTGGAGGGTCTCCCTCCTGATCGAATTGTTCCAACTGTGGGACTAAATATTGGTCGTATTGAAGCATCAAATACCAAACTTGTGTTCTGGGACCTAGGGGGTCAGCCTGGTCTTCGCTCAATCTGGGAGAAATATTATGAAGAAGCACATGCTGTGGTCTTTGTAATTGATGCCACTTGTCCATCACGTTTTGAAGATTCAAAATCTGCATTAGAAAAAGTTCTTCGGCATGAGGATTTGCAAGGAGCTCCTCTATTGATTTTGGCCAACAAGCAGGATGTTGCTGATTCTGTGTCAGCTGAGGAACTTGCTCGATATCTGGATCTTAAAAAGTTGGATGAAAGGGTTTTTATGTTTGAAGCTGTTTCAGCATACGATGGGATGGGGATTAAAGAAAGCGTCGAGTGGTTGGTGGAGGTAATGGAGAGAAGCAAGAGAACTGAAACACTGAGAGCCCGAGCAGGCCCGGCTTCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

203

Amino Acids

22.81

Weight (kDa)

5.53

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 10 - 182 1.2e-49 ADP-ribosylation factor family
SRPRB PF09439 19 - 142 6.3e-06 Signal recognition particle receptor beta subunit
Ras PF00071 20 - 184 1.1e-11 Ras family
Roc PF08477 20 - 136 3.6e-10 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 20 - 134 1.4e-06 50S ribosome-binding GTPase
G-alpha PF00503 53 - 137 6.2e-06 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 459
AcoI YGGCCR 1 cut(s) 393
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 167
AgsI TTSAA 5 cut(s) 112, 191, 323, 330, 488
AjnI CCWGG 1 cut(s) 235
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 3 cut(s) 377, 428, 491
AluI AGCT 3 cut(s) 377, 428, 491
Alw21I GWGCWC 2 cut(s) 63, 379
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 1 cut(s) 459
Ama87I CYCGRG 1 cut(s) 585
AoxI GGCC 2 cut(s) 393, 592
Asp700I GAANNNNTTC 1 cut(s) 348
AspA2I CCTAGG 1 cut(s) 223
AspS9I GGNCC 2 cut(s) 220, 593
AsuC2I CCSGG 1 cut(s) 596
AvaI CYCGRG 1 cut(s) 585
AvaII GGWCC 1 cut(s) 220
AvrII CCTAGG 1 cut(s) 223
BalI TGGCCA 1 cut(s) 395
BanII GRGCYC 2 cut(s) 379, 586
BarI GAAGNNNNNNTAC 2 cut(s) 104, 136
BbsI GAAGAC 2 cut(s) 95, 233
Bbv12I GWGCWC 2 cut(s) 63, 379
BbvCI CCTCAGC 1 cut(s) 429
BccI CCATC 3 cut(s) 319, 500, 505
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 1 cut(s) 596
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 2 cut(s) 224, 607
BlnI CCTAGG 1 cut(s) 223
Bme1390I CCNGG 2 cut(s) 237, 596
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 585
BmgT120I GGNCC 2 cut(s) 220, 593
BmiI GGNNCC 1 cut(s) 221
BmrFI CCNGG 2 cut(s) 237, 596
BmsI GCATC 2 cut(s) 203, 289
BpiI GAAGAC 2 cut(s) 95, 233
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 596
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 223
Bsc4I CCNNNNNNNGG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 3 cut(s) 412, 472, 516
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMII CTCAG 2 cut(s) 420, 569
BseRI GAGGAG 1 cut(s) 369
BseYI CCCAGC 1 cut(s) 83
BshFI GGCC 2 cut(s) 395, 594
BsiHKAI GWGCWC 2 cut(s) 63, 379
BsiHKCI CYCGRG 1 cut(s) 585
BsiSI CCGG 1 cut(s) 596
BslFI GGGAC 2 cut(s) 183, 233
BslI CCNNNNNNNGG 1 cut(s) 167
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 2 cut(s) 183, 233
BsnI GGCC 2 cut(s) 395, 594
Bso31I GGTCTC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 585
Bsp1286I GDGCHC 3 cut(s) 63, 379, 586
Bsp143I GATC 2 cut(s) 148, 451
BspANI GGCC 2 cut(s) 395, 594
BspCNI CTCAG 2 cut(s) 421, 570
BspLI GGNNCC 1 cut(s) 221
BspPI GGATC 1 cut(s) 459
BspTNI GGTCTC 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 2 cut(s) 148, 451
BssT1I CCWWGG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 592
BstDEI CTNAG 2 cut(s) 429, 578
BstF5I GGATG 3 cut(s) 412, 472, 516
BstKTI GATC 2 cut(s) 151, 454
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 2 cut(s) 148, 451
BstMWI GCNNNNNNNGC 2 cut(s) 497, 596
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 235, 594
BstV2I GAAGAC 2 cut(s) 95, 233
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BsuRI GGCC 2 cut(s) 395, 594
BtsCI GGATG 3 cut(s) 412, 472, 516
BtsIMutI CAGTG 1 cut(s) 575
Cac8I GCNNGC 1 cut(s) 592
Cfr13I GGNCC 2 cut(s) 220, 593
CseI GACGC 1 cut(s) 517
Csp6I GTAC 1 cut(s) 121
CviAII CATG 3 cut(s) 56, 278, 359
CviJI RGCY 9 cut(s) 83, 235, 377, 395, 428, 491, 584, 594, 599
CviKI_1 RGCY 9 cut(s) 83, 235, 377, 395, 428, 491, 584, 594, 599
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 2 cut(s) 429, 578
DpnI GATC 2 cut(s) 150, 453
DpnII GATC 2 cut(s) 148, 451
EaeI YGGCCR 1 cut(s) 393
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 223
Eco24I GRGCYC 2 cut(s) 379, 586
Eco31I GGTCTC 1 cut(s) 143
Eco32I GATATC 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 377
Eco88I CYCGRG 1 cut(s) 585
EcoICRI GAGCTC 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 235
EcoRV GATATC 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 223
EcoT38I GRGCYC 2 cut(s) 379, 586
ErhI CCWWGG 1 cut(s) 223
FaeI CATG 3 cut(s) 59, 281, 362
FaiI YATR 9 cut(s) 25, 33, 35, 57, 267, 279, 360, 482, 502
FaqI GGGAC 2 cut(s) 183, 233
FatI CATG 3 cut(s) 55, 277, 358
FokI GGATG 3 cut(s) 419, 479, 523
FriOI GRGCYC 2 cut(s) 379, 586
FspBI CTAG 2 cut(s) 224, 607
GsaI CCCAGC 1 cut(s) 87
HaeIII GGCC 2 cut(s) 395, 594
HapII CCGG 1 cut(s) 596
HgaI GACGC 1 cut(s) 517
Hin1II CATG 3 cut(s) 59, 281, 362
HinfI GANTC 3 cut(s) 72, 326, 416
HpaII CCGG 1 cut(s) 596
Hpy188III TCNNGA 2 cut(s) 146, 449
Hpy99I CGWCG 1 cut(s) 533
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 2 cut(s) 338, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 497, 596
HpyF3I CTNAG 2 cut(s) 429, 578
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 3 cut(s) 59, 281, 362
Kzo9I GATC 2 cut(s) 148, 451
LmnI GCTCC 2 cut(s) 374, 382
LpnPI CCDG 9 cut(s) 69, 159, 202, 222, 238, 249, 389, 434, 576
LweI GCATC 2 cut(s) 203, 289
MaeI CTAG 2 cut(s) 224, 607
MaeII ACGT 1 cut(s) 316
MalI GATC 2 cut(s) 150, 453
MboI GATC 2 cut(s) 148, 451
MboII GAAGA 5 cut(s) 100, 233, 281, 335, 344
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 3 cut(s) 63, 379, 586
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 2 cut(s) 153, 294
MluNI TGGCCA 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 185, 444
MnlI CCTC 6 cut(s) 127, 153, 355, 390, 424, 538
Mox20I TGGCCA 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 348
MscI TGGCCA 1 cut(s) 395
MseI TTAA 2 cut(s) 456, 519
Msp20I TGGCCA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 1 cut(s) 596
MspR9I CCNGG 2 cut(s) 237, 596
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 2 cut(s) 497, 596
NciI CCSGG 1 cut(s) 596
NdeII GATC 2 cut(s) 148, 451
NlaIII CATG 3 cut(s) 59, 281, 362
NlaIV GGNNCC 1 cut(s) 221
NspI RCATGY 1 cut(s) 281
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 72, 326, 416
PpuMI RGGWCCY 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 221
PspPI GGNCC 2 cut(s) 220, 593
PspPPI RGGWCCY 1 cut(s) 220
PsuI RGATCY 1 cut(s) 451
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SacI GAGCTC 1 cut(s) 379
SaqAI TTAA 2 cut(s) 456, 519
Sau3AI GATC 2 cut(s) 148, 451
Sau96I GGNCC 2 cut(s) 220, 593
ScrFI CCNGG 2 cut(s) 237, 596
SduI GDGCHC 3 cut(s) 63, 379, 586
SetI ASST 6 cut(s) 225, 319, 379, 430, 493, 549
SfaNI GCATC 2 cut(s) 203, 289
SinI GGWCC 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 2 cut(s) 153, 294
SspI AATATT 2 cut(s) 178, 263
SspMI CTAG 2 cut(s) 224, 607
SstI GAGCTC 1 cut(s) 379
StyD4I CCNGG 2 cut(s) 235, 594
StyI CCWWGG 1 cut(s) 223
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 1 cut(s) 319
TaqI TCGA 4 cut(s) 75, 151, 442, 531
TasI AATT 2 cut(s) 153, 294
TatI WGTACW 1 cut(s) 120
TfiI GAWTC 3 cut(s) 72, 326, 416
Tru1I TTAA 2 cut(s) 456, 519
Tru9I TTAA 2 cut(s) 456, 519
TscAI CASTG 1 cut(s) 582
TspDTI ATGAA 3 cut(s) 44, 282, 483
TspGWI ACGGA 2 cut(s) 33, 62
TspRI CASTG 1 cut(s) 582
VpaK11BI GGWCC 1 cut(s) 220
XceI RCATGY 1 cut(s) 281
XmaJI CCTAGG 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 2 cut(s) 224, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.