MD07G1267000.v1.1
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010068
Physical Location & Seq
Forward (+)
33503529 .. 33506524
2996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1267000.v1.1.491

Sequence Viewer

Length: 609 bp
ATGTTTTCATTGTTTTATGGATTCTGGAAATATATGTTCAGTAAGACGGAGTTTCATGTACTCATTCTTGGAATCGACAAGGCTGGGAAGACGACTTTACTCGAGAAGTTGAAGTCAATTTACTCAAACTTAGAAGGTCTCCCTCCTGATCGGATTGTTCCAACCGTGGGACTTAATATTGGTCGTATTGAAGCATCAAATACCAAACTTGTGTTCTGGGACCTAGGCGGTCAGCCTGGTCTTCGCTCAATCTGGGAGAAATATTATGAAGAAGCACATGCTGTGGTCTATGTAATTGATGCCACTTGTCCATCACGTTTTGAAGATTCAAAATCTGCATTGGAAAAAGTTCTTCGGCATGAGGATTTGCAAGGAGCTCCTCTATTGATTTTGGCCAACAAGCAGGATGTTGCTGATTCTGTATCAGCCGAGGAACTTGCTCGATATCTGGATCTTAAAAAGTTGGATGAAAGGGTTTATATGTTTGAAGCCGTTTCAGCATACGATGGGATGGGGATTAAAGAAAGCGTGGAGTGGTTGGTGGCGGTAATGGAGAGAAGCAAGAGAACTGAAACGTTGAGAGCCCGTGCAGGCCCGGCATCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

203

Amino Acids

22.8

Weight (kDa)

5.73

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 11 - 182 4e-49 ADP-ribosylation factor family
Ras PF00071 20 - 183 3.9e-11 Ras family
Roc PF08477 20 - 136 8.9e-10 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 20 - 134 3.8e-06 50S ribosome-binding GTPase
Gtr1_RagA PF04670 20 - 145 3.3e-06 Gtr1/RagA G protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 228, 545
AclI AACGTT 1 cut(s) 575
AclWI GGATC 1 cut(s) 459
AcoI YGGCCR 1 cut(s) 393
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 167
AgsI TTSAA 5 cut(s) 112, 191, 323, 330, 488
AjnI CCWGG 1 cut(s) 235
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 1 cut(s) 459
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 2 cut(s) 393, 592
Asp700I GAANNNNTTC 1 cut(s) 348
AspA2I CCTAGG 1 cut(s) 223
AspS9I GGNCC 2 cut(s) 220, 593
AsuC2I CCSGG 1 cut(s) 596
AvaI CYCGRG 1 cut(s) 101
AvaII GGWCC 1 cut(s) 220
AvrII CCTAGG 1 cut(s) 223
BalI TGGCCA 1 cut(s) 395
BanII GRGCYC 2 cut(s) 379, 586
BarI GAAGNNNNNNTAC 2 cut(s) 104, 136
BbsI GAAGAC 2 cut(s) 95, 233
Bbv12I GWGCWC 1 cut(s) 379
BccI CCATC 3 cut(s) 319, 500, 505
BceAI ACGGC 1 cut(s) 476
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 1 cut(s) 596
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 2 cut(s) 224, 607
BlnI CCTAGG 1 cut(s) 223
Bme1390I CCNGG 2 cut(s) 237, 596
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 101
BmgT120I GGNCC 2 cut(s) 220, 593
BmiI GGNNCC 1 cut(s) 221
BmrFI CCNGG 2 cut(s) 237, 596
BmsI GCATC 2 cut(s) 203, 289
BpiI GAAGAC 2 cut(s) 95, 233
BpuMI CCSGG 1 cut(s) 596
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 3 cut(s) 165, 223, 429
Bsc4I CCNNNNNNNGG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 165, 223, 429
BseGI GGATG 3 cut(s) 412, 472, 516
BseLI CCNNNNNNNGG 1 cut(s) 167
BseRI GAGGAG 1 cut(s) 369
BseYI CCCAGC 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 609
BshFI GGCC 2 cut(s) 395, 594
BsiHKAI GWGCWC 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 1 cut(s) 596
BslFI GGGAC 2 cut(s) 183, 233
BslI CCNNNNNNNGG 1 cut(s) 167
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 2 cut(s) 183, 233
BsnI GGCC 2 cut(s) 395, 594
Bso31I GGTCTC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 379, 586
Bsp143I GATC 2 cut(s) 148, 451
BspACI CCGC 2 cut(s) 228, 545
BspANI GGCC 2 cut(s) 395, 594
BspLI GGNNCC 1 cut(s) 221
BspPI GGATC 1 cut(s) 459
BspTNI GGTCTC 1 cut(s) 143
BssECI CCNNGG 3 cut(s) 165, 223, 429
BssMI GATC 2 cut(s) 148, 451
BssT1I CCWWGG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 592
BstDEI CTNAG 1 cut(s) 130
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 412, 472, 516
BstKTI GATC 2 cut(s) 151, 454
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 2 cut(s) 148, 451
BstMWI GCNNNNNNNGC 2 cut(s) 497, 596
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 235, 594
BstV2I GAAGAC 2 cut(s) 95, 233
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BsuRI GGCC 2 cut(s) 395, 594
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 3 cut(s) 412, 472, 516
Cac8I GCNNGC 1 cut(s) 592
Cfr13I GGNCC 2 cut(s) 220, 593
Csp6I GTAC 1 cut(s) 59
CviAII CATG 3 cut(s) 56, 278, 359
CviJI RGCY 8 cut(s) 83, 235, 377, 395, 428, 491, 584, 594
CviKI_1 RGCY 8 cut(s) 83, 235, 377, 395, 428, 491, 584, 594
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 1 cut(s) 130
DpnI GATC 2 cut(s) 150, 453
DpnII GATC 2 cut(s) 148, 451
EaeI YGGCCR 1 cut(s) 393
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 223
Eco24I GRGCYC 2 cut(s) 379, 586
Eco31I GGTCTC 1 cut(s) 143
Eco32I GATATC 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 377
Eco88I CYCGRG 1 cut(s) 101
EcoICRI GAGCTC 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 235
EcoRV GATATC 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 223
EcoT38I GRGCYC 2 cut(s) 379, 586
ErhI CCWWGG 1 cut(s) 223
FaeI CATG 3 cut(s) 59, 281, 362
FaqI GGGAC 2 cut(s) 183, 233
FatI CATG 3 cut(s) 55, 277, 358
FokI GGATG 3 cut(s) 419, 479, 523
FriOI GRGCYC 2 cut(s) 379, 586
FspBI CTAG 2 cut(s) 224, 607
GsaI CCCAGC 1 cut(s) 87
HaeIII GGCC 2 cut(s) 395, 594
HapII CCGG 1 cut(s) 596
Hin1II CATG 3 cut(s) 59, 281, 362
HinfI GANTC 4 cut(s) 21, 72, 326, 416
HpaII CCGG 1 cut(s) 596
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 4 cut(s) 25, 103, 146, 449
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 2 cut(s) 316, 575
HpyCH4V TGCA 3 cut(s) 338, 370, 590
HpyF10VI GCNNNNNNNGC 2 cut(s) 497, 596
HpyF3I CTNAG 1 cut(s) 130
HpySE526I ACGT 2 cut(s) 316, 575
Hsp92II CATG 3 cut(s) 59, 281, 362
Kzo9I GATC 2 cut(s) 148, 451
LmnI GCTCC 2 cut(s) 374, 382
LweI GCATC 2 cut(s) 203, 289
MaeI CTAG 2 cut(s) 224, 607
MaeII ACGT 2 cut(s) 316, 575
MalI GATC 2 cut(s) 150, 453
MboI GATC 2 cut(s) 148, 451
MboII GAAGA 5 cut(s) 100, 233, 281, 335, 344
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 2 cut(s) 379, 586
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 2 cut(s) 117, 294
MluNI TGGCCA 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 185, 444
MnlI CCTC 4 cut(s) 153, 355, 390, 424
Mox20I TGGCCA 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 348
MscI TGGCCA 1 cut(s) 395
MseI TTAA 3 cut(s) 174, 456, 519
Msp20I TGGCCA 1 cut(s) 395
MspI CCGG 1 cut(s) 596
MspR9I CCNGG 2 cut(s) 237, 596
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 2 cut(s) 497, 596
NciI CCSGG 1 cut(s) 596
NdeII GATC 2 cut(s) 148, 451
NlaIII CATG 3 cut(s) 59, 281, 362
NlaIV GGNNCC 1 cut(s) 221
NmeAIII GCCGAG 1 cut(s) 454
NspI RCATGY 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 101
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 4 cut(s) 21, 72, 326, 416
PpuMI RGGWCCY 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 379
Psp1406I AACGTT 1 cut(s) 575
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 221
PspPI GGNCC 2 cut(s) 220, 593
PspPPI RGGWCCY 1 cut(s) 220
PsuI RGATCY 1 cut(s) 451
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
SacI GAGCTC 1 cut(s) 379
SaqAI TTAA 3 cut(s) 174, 456, 519
Sau3AI GATC 2 cut(s) 148, 451
Sau96I GGNCC 2 cut(s) 220, 593
ScrFI CCNGG 2 cut(s) 237, 596
SduI GDGCHC 2 cut(s) 379, 586
SetI ASST 5 cut(s) 139, 225, 319, 379, 578
SfaNI GCATC 2 cut(s) 203, 289
Sfr274I CTCGAG 1 cut(s) 101
SinI GGWCC 1 cut(s) 220
SlaI CTCGAG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 2 cut(s) 117, 294
SsiI CCGC 2 cut(s) 228, 545
SspI AATATT 2 cut(s) 178, 263
SspMI CTAG 2 cut(s) 224, 607
SstI GAGCTC 1 cut(s) 379
StyD4I CCNGG 2 cut(s) 235, 594
StyI CCWWGG 1 cut(s) 223
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 2 cut(s) 319, 578
TaqI TCGA 3 cut(s) 75, 102, 442
TasI AATT 2 cut(s) 117, 294
TatI WGTACW 1 cut(s) 58
TfiI GAWTC 4 cut(s) 21, 72, 326, 416
Tru1I TTAA 3 cut(s) 174, 456, 519
Tru9I TTAA 3 cut(s) 174, 456, 519
TspDTI ATGAA 3 cut(s) 44, 282, 483
TspGWI ACGGA 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 220
XceI RCATGY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 101
XmaJI CCTAGG 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 2 cut(s) 224, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.