Rroxscaffold_4G00281550
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010068
Physical Location & Seq
Reverse (-)
3805115 .. 3807754
2640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281550.1

Sequence Viewer

Length: 612 bp
ATGTTTTCGTTGTTTTATGGACTCTGGAAGTATATGTTTAGCAAGACAGAGTTTCATGTACTCATTCTTGGAATTGACAAGGCTGGGAAAACGACTTTGCTGGAGAAGTTGAAATCAGTTTACTCAAATTTGGAAGGTCTCCCTCCTGATCGAATTGTTCCAACTGTGGGACTTAATATTGGTCGTATCGAAGTTTCAAATACTAAACTTGTGTTCTGGGACCTGGGAGGTCAGCCTGGTCTTCGCTCAATCTGGGAGAAATATTATGAAGAAGCGCATGCTGTGGTCTATGTAATTGATGCTACTTGCCCATCACGTTTTGAAGATTCAAAATCTGCATTGGAAAAAGTTCTTCGGCACGAGGATCTGCAAGGAGCTCCTCTGTTGATATTGGCCAATAAGCAGGATCTTAATGATGCTGTATCATCCGAGGAACTTGCTCGATATCTAGATCTTAAAAAGTTGGATGAAAGAGTTTACATGTTTGAAGCTGTTTCAGCATATGACGGGATGGGGATTAAAGAAAGCGTAGAGTGGTTGGTGGAGGTAATGGAGAGAAGCAAGAGAACTGAAACGTTGAGAGCTCGTGCAGGAGGCCCAGCCTCGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

203

Amino Acids

22.95

Weight (kDa)

5.53

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 10 - 182 2e-50 ADP-ribosylation factor family
Ras PF00071 20 - 184 1e-11 Ras family
Roc PF08477 20 - 136 6.4e-10 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 20 - 134 2.3e-06 50S ribosome-binding GTPase
Gtr1_RagA PF04670 20 - 145 2.6e-06 Gtr1/RagA G protein conserved region
G-alpha PF00503 53 - 137 2.3e-06 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 575
AclWI GGATC 2 cut(s) 372, 414
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 127
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 2 cut(s) 167, 604
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 5 cut(s) 112, 198, 323, 330, 488
AjnI CCWGG 2 cut(s) 222, 235
AluBI AGCT 3 cut(s) 377, 491, 584
AluI AGCT 3 cut(s) 377, 491, 584
Alw21I GWGCWC 2 cut(s) 379, 586
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 2 cut(s) 372, 414
AoxI GGCC 2 cut(s) 393, 595
ApoI RAATTY 1 cut(s) 127
Asp700I GAANNNNTTC 1 cut(s) 348
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 2 cut(s) 220, 596
AvaII GGWCC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 395
BanII GRGCYC 2 cut(s) 379, 586
BauI CACGAG 2 cut(s) 359, 585
BbsI GAAGAC 1 cut(s) 233
Bbv12I GWGCWC 2 cut(s) 379, 586
BccI CCATC 2 cut(s) 319, 505
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 2 cut(s) 224, 237
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 1 cut(s) 449
BglII AGATCT 1 cut(s) 451
Bme1390I CCNGG 2 cut(s) 224, 237
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 2 cut(s) 220, 596
BmiI GGNNCC 1 cut(s) 221
BmrFI CCNGG 2 cut(s) 224, 237
BmsI GCATC 2 cut(s) 289, 406
BpiI GAAGAC 1 cut(s) 233
BpmI CTGGAG 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 3 cut(s) 223, 429, 603
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 604
BseBI CCWGG 2 cut(s) 224, 237
BseDI CCNNGG 3 cut(s) 223, 429, 603
BseGI GGATG 3 cut(s) 425, 472, 516
BseLI CCNNNNNNNGG 2 cut(s) 167, 604
BseRI GAGGAG 1 cut(s) 369
BseYI CCCAGC 2 cut(s) 83, 598
BsgI GTGCAG 1 cut(s) 609
BshFI GGCC 2 cut(s) 395, 597
BsiHKAI GWGCWC 2 cut(s) 379, 586
BslFI GGGAC 2 cut(s) 183, 233
BslI CCNNNNNNNGG 2 cut(s) 167, 604
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 2 cut(s) 183, 233
BsnI GGCC 2 cut(s) 395, 597
Bso31I GGTCTC 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 379, 586
Bsp143I GATC 4 cut(s) 148, 364, 406, 451
BspANI GGCC 2 cut(s) 395, 597
BspLI GGNNCC 1 cut(s) 221
BspPI GGATC 2 cut(s) 372, 414
BspTNI GGTCTC 1 cut(s) 143
BssECI CCNNGG 3 cut(s) 223, 429, 603
BssMI GATC 4 cut(s) 148, 364, 406, 451
BssSI CACGAG 2 cut(s) 359, 585
Bst2BI CACGAG 2 cut(s) 359, 585
Bst2UI CCWGG 2 cut(s) 224, 237
Bst4CI ACNGT 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 1 cut(s) 609
BstF5I GGATG 3 cut(s) 425, 472, 516
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 4 cut(s) 151, 367, 409, 454
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 4 cut(s) 148, 364, 406, 451
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 2 cut(s) 224, 237
BstNSI RCATGY 2 cut(s) 281, 484
BstSCI CCNGG 2 cut(s) 222, 235
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 3 cut(s) 364, 406, 451
BstYI RGATCY 3 cut(s) 364, 406, 451
BsuRI GGCC 2 cut(s) 395, 597
BtsCI GGATG 3 cut(s) 425, 472, 516
Cac8I GCNNGC 1 cut(s) 279
CfoI GCGC 1 cut(s) 277
Cfr13I GGNCC 2 cut(s) 220, 596
Csp6I GTAC 1 cut(s) 59
CviAII CATG 3 cut(s) 56, 278, 481
CviJI RGCY 9 cut(s) 83, 235, 377, 395, 491, 584, 597, 602, 608
CviKI_1 RGCY 9 cut(s) 83, 235, 377, 395, 491, 584, 597, 602, 608
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 1 cut(s) 609
DpnI GATC 4 cut(s) 150, 366, 408, 453
DpnII GATC 4 cut(s) 148, 364, 406, 451
EaeI YGGCCR 1 cut(s) 393
Ecl136II GAGCTC 2 cut(s) 377, 584
Eco24I GRGCYC 2 cut(s) 379, 586
Eco31I GGTCTC 1 cut(s) 143
Eco32I GATATC 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 2 cut(s) 377, 584
EcoICRI GAGCTC 2 cut(s) 377, 584
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 2 cut(s) 222, 235
EcoRV GATATC 1 cut(s) 446
EcoT38I GRGCYC 2 cut(s) 379, 586
FaeI CATG 3 cut(s) 59, 281, 484
FaqI GGGAC 2 cut(s) 183, 233
FatI CATG 3 cut(s) 55, 277, 480
FauNDI CATATG 1 cut(s) 502
FokI GGATG 3 cut(s) 412, 479, 523
FriOI GRGCYC 2 cut(s) 379, 586
FspBI CTAG 1 cut(s) 449
GlaI GCGC 1 cut(s) 276
GsaI CCCAGC 2 cut(s) 87, 602
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 2 cut(s) 395, 597
HhaI GCGC 1 cut(s) 277
Hin1II CATG 3 cut(s) 59, 281, 484
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HinfI GANTC 2 cut(s) 21, 326
Hpy166II GTNNAC 2 cut(s) 121, 478
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 3 cut(s) 25, 146, 449
Hpy8I GTNNAC 2 cut(s) 121, 478
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 2 cut(s) 316, 575
HpyCH4V TGCA 3 cut(s) 338, 370, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 1 cut(s) 609
HpySE526I ACGT 2 cut(s) 316, 575
Hsp92II CATG 3 cut(s) 59, 281, 484
HspAI GCGC 1 cut(s) 275
Kzo9I GATC 4 cut(s) 148, 364, 406, 451
LmnI GCTCC 2 cut(s) 374, 382
LweI GCATC 2 cut(s) 289, 406
MaeI CTAG 1 cut(s) 449
MaeII ACGT 2 cut(s) 316, 575
MalI GATC 4 cut(s) 150, 366, 408, 453
MboI GATC 4 cut(s) 148, 364, 406, 451
MboII GAAGA 4 cut(s) 233, 281, 335, 344
MflI RGATCY 3 cut(s) 364, 406, 451
MhlI GDGCHC 2 cut(s) 379, 586
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 4 cut(s) 72, 127, 153, 294
MluNI TGGCCA 1 cut(s) 395
MlyI GAGTC 1 cut(s) 15
MmeI TCCRAC 2 cut(s) 185, 444
MnlI CCTC 7 cut(s) 153, 221, 355, 390, 424, 538, 587
Mox20I TGGCCA 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 348
MscI TGGCCA 1 cut(s) 395
MseI TTAA 4 cut(s) 174, 411, 456, 519
Msp20I TGGCCA 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 224, 237
MvaI CCWGG 2 cut(s) 224, 237
MwoI GCNNNNNNNGC 1 cut(s) 497
NdeI CATATG 1 cut(s) 502
NdeII GATC 4 cut(s) 148, 364, 406, 451
NlaIII CATG 3 cut(s) 59, 281, 484
NlaIV GGNNCC 1 cut(s) 221
NmeAIII GCCGAG 1 cut(s) 584
NspI RCATGY 2 cut(s) 281, 484
PaeI GCATGC 1 cut(s) 281
PciI ACATGT 1 cut(s) 480
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 326
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PpuMI RGGWCCY 1 cut(s) 220
PscI ACATGT 1 cut(s) 480
Psp124BI GAGCTC 2 cut(s) 379, 586
Psp1406I AACGTT 1 cut(s) 575
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 2 cut(s) 222, 235
PspFI CCCAGC 2 cut(s) 83, 598
PspGI CCWGG 2 cut(s) 222, 235
PspN4I GGNNCC 1 cut(s) 221
PspPI GGNCC 2 cut(s) 220, 596
PspPPI RGGWCCY 1 cut(s) 220
PsuI RGATCY 3 cut(s) 364, 406, 451
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
SacI GAGCTC 2 cut(s) 379, 586
SaqAI TTAA 4 cut(s) 174, 411, 456, 519
Sau3AI GATC 4 cut(s) 148, 364, 406, 451
Sau96I GGNCC 2 cut(s) 220, 596
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 2 cut(s) 224, 237
SduI GDGCHC 2 cut(s) 379, 586
SetI ASST 9 cut(s) 139, 225, 232, 319, 379, 493, 549, 578, 586
SfaNI GCATC 2 cut(s) 289, 406
SinI GGWCC 1 cut(s) 220
SphI GCATGC 1 cut(s) 281
Sse9I AATT 4 cut(s) 72, 127, 153, 294
SspI AATATT 2 cut(s) 178, 263
SspMI CTAG 1 cut(s) 449
SstI GAGCTC 2 cut(s) 379, 586
StyD4I CCNGG 2 cut(s) 222, 235
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 2 cut(s) 319, 578
TaqI TCGA 3 cut(s) 151, 189, 442
TasI AATT 4 cut(s) 72, 127, 153, 294
TatI WGTACW 1 cut(s) 58
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 4 cut(s) 174, 411, 456, 519
Tru9I TTAA 4 cut(s) 174, 411, 456, 519
TspDTI ATGAA 3 cut(s) 44, 282, 483
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 1 cut(s) 127
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 2 cut(s) 281, 484
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.