MD01G1199300.v1.1

methyl-CpG-binding domain-containing protein 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003930
Physical Location & Seq
Reverse (-)
29654974 .. 29658970
3997 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1199300.v1.1.491

Sequence Viewer

Length: 2091 bp
ATGGCGGACCAGAACTCCGAGGAATGGCTCCCAGCTGGATGGACAGTGGAGGTCAAAGTTAGAAAAAACGGCAGAAGAGACAAGTATTACTACGAATCCTCAAGTGGACATAGATTTAGTTCCAGGGCGGAGGTATCTCGCTATCTCAGCACCTCCAAAACCAGTCGTGAAGACAAACAGAAAATTGAGGAGACTCTCAAGCGACCTTCAGATGATGTTGTAGTTGAGAAGACTGTAGCAGAAAGACTGCCTCCAGGATGGATCAAAGAAATCCGGATGACGAAAAAGGCTCGTAAGATTAGGAGAGACACGTGTCATTATATTGCTCCTGTAAGTGAAAAAGTATTCCGCTCTCTCAGGGATGTGCATCGCTATCTTGAAAATGAGGAGCCAGGAAGGTTAAATGTCGAGCCGAGTGGCAGCAGTGATAAGGAATTGGAAGATGAAAAATCCTCTAAATTAGACAACGCAAGTTTGAACTTCAACGAGACAACGAAGGATGGAGAGATACTTAATTGTTCATGTAACGGAGAAGGCCTGCACCTTCCTGAACATACTTCTGATCAGAGTGGGGTGGGCATTGAATTAGGCAGCTTAAATCCTTCAGAAGCCAAAGGTTCAGAGCAAAATGAACAAGACGGTGATTCCACAAAAAGTGAATGCATTTCAGCTCCTGCTGGTGGAGACCCGCCAGATAACCAATGCCTGGAAAGTGGGATGATAAAACATGAAATAAACCGTTCTGGCCTCTGCAAATCAAAGAACAAGAAAGAGCTTATCTTGCCTCGCCGTGCTTCAAAGCGACTAGCTGGAGTTGAAGTTGGTCCATTTCCAGAACTGAAAACAAGTATTCGAGCCCGTCGAGCTGCAACTGAACAGTCAGGGGAGGGAACGCGCCATACAACCGAAGGCCCGTCCCTTCCTGAACACAGTTCTGATCAGAGTGAGGTGGGCAGCTTAAATCCTCCAGAATCCAAAGGTTCAGAGCAAAAAGAACAGGACTGTGATTCCACAAAAAATGAATGTATTTCAATTCCTGCAGGTGGAGGTCCGCCAGATAAGCAATGCCTGGAAACTGGTCTGATAAAACATGAAGGGAAAAAGACCCATCTTGACCTTTGCAAATCAAAGAACAAGATGGAGCCTATCGTTCCTCGCCGTGCTTCAATGAGACTAGCTGGAGTTGAAGCTGGTCCAGTTCCAGAAGTGAAACCAAGTACTCCAGCACGTCGATTTGCTGTTAAACAGTCAGGGGAGGGAGTACACCGTACAACTGAAGGTTCTTCCCTTGGTAATTTGCCTAGTTGTGCATTCCAGCAGCCTGATTATCATGAGGTTGAGCCAGAAAAGCATATTCAAAATGTGGAACAAGCAATTAATGGTGACGCTAAGCAAAGGTGTGCTTCTGTTTTCCCATTGGGGAGCCGAAGTAGCCCAGAAGAGCATGCAGGAAAACTTGAAACCGATTATAAGGCTGAAGAGAAGCCAGGACTTCAACCAGACTTACCTCTCGGCGATTTCTTCACGGATCCTTGCATTGCATTTGCAATAGAGACTCTCACTGGAATAGGTTTTGACAATGCCAGGAGCGATGGTGACCATTCTTCAGGAGGACTAACTACACCAAAAGATCATCATGGGGACATAGACAATAAGAGCAGTGGGAAGCAGGAAAGCGGCAACCTTTTTATTCCAGAACGCTGGGGGAAAGTTGGAAAGGATGATGATGCTGGACAGAAGTCAGGATCCCCTACTGAGTTGCCTTTTGGGGGTTCATGGCCCGATCCATGCATTGAATTTGCTATAAAAACTCTCACGGGTGCAATCCCAGTAGACTACGATCCGCACATCGAGGAATACTTTCAGCAGCAACTAAGCTCATCAAAAACTCAAGGAACAAGCGACTTGACCTTGAAAAATGTTGGTCTAGATAACTTCTGCCAAACGGATGATTTGTGCGAGCAGTTCTGTCATGCGGAGGTCCCTGTGCTTAAGAAACAAGCGCTTTCTGAACCTCGACACACCAGGAGCCGAAGTTTACACAGTTCCGGTGGATCAGATGTAAACCAAAAGGGGAGGCAGAGACACTGA

Protein Analysis

697

Amino Acids

76.42

Weight (kDa)

5.79

Isoelectric Point (pI)

61.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 6 - 53 1.6e-07 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1470
AarI CACCTGC 1 cut(s) 1031
Acc36I ACCTGC 1 cut(s) 1031
AccB7I CCANNNNNTGG 1 cut(s) 706
AccBSI CCGCTC 1 cut(s) 351
AccI GTMKAC 1 cut(s) 1833
AccII CGCG 1 cut(s) 895
AccIII TCCGGA 1 cut(s) 273
AciI CCGC 8 cut(s) 5, 128, 349, 689, 1052, 1677, 1844, 1976
AclWI GGATC 8 cut(s) 269, 1523, 1536, 1740, 1753, 1778, 1835, 2062
AcsI RAATTY 1 cut(s) 1796
AcuI CTGAAG 5 cut(s) 192, 588, 1296, 1497, 1590
AcvI CACGTG 1 cut(s) 312
AfaI GTAC 3 cut(s) 1219, 1263, 1270
AfeI AGCGCT 1 cut(s) 2004
AfiI CCNNNNNNNGG 5 cut(s) 24, 680, 706, 1043, 1769
AflII CTTAAG 1 cut(s) 1991
AflIII ACRYGT 2 cut(s) 309, 311
AjiI CACGTC 1 cut(s) 1229
AjnI CCWGG 8 cut(s) 122, 253, 391, 705, 1068, 1486, 1583, 2024
Alw26I GTCTC 8 cut(s) 72, 185, 300, 482, 678, 1165, 1547, 2077
AlwI GGATC 8 cut(s) 269, 1523, 1536, 1740, 1753, 1778, 1835, 2062
AlwNI CAGNNNCTG 1 cut(s) 674
Aor13HI TCCGGA 1 cut(s) 273
Aor51HI AGCGCT 1 cut(s) 2004
AoxI GGCC 4 cut(s) 535, 745, 910, 1778
ApeKI GCWGC 6 cut(s) 420, 591, 866, 954, 1318, 1867
ApoI RAATTY 1 cut(s) 1796
AseI ATTAAT 1 cut(s) 1377
Asp700I GAANNNNTTC 1 cut(s) 1860
AspLEI GCGC 2 cut(s) 897, 2005
AspS9I GGNCC 7 cut(s) 7, 824, 911, 1049, 1193, 1779, 1981
AsuHPI GGTGA 3 cut(s) 653, 1394, 1607
AvaII GGWCC 5 cut(s) 7, 824, 1049, 1193, 1981
BamHI GGATCC 2 cut(s) 1528, 1745
BanII GRGCYC 1 cut(s) 859
BbrPI CACGTG 1 cut(s) 312
BbsI GAAGAC 2 cut(s) 177, 236
BbvI GCAGC 6 cut(s) 432, 603, 853, 966, 1330, 1879
BccI CCATC 6 cut(s) 33, 252, 494, 1116, 1132, 1586
BceAI ACGGC 3 cut(s) 85, 774, 1143
BciT130I CCWGG 8 cut(s) 124, 255, 393, 707, 1070, 1488, 1585, 2026
BclI TGATCA 2 cut(s) 562, 937
BcoDI GTCTC 8 cut(s) 72, 185, 300, 482, 678, 1165, 1547, 2077
BfaI CTAG 4 cut(s) 806, 1175, 1302, 1928
BfmI CTRYAG 2 cut(s) 234, 1038
BfoI RGCGCY 1 cut(s) 2006
BfrI CTTAAG 1 cut(s) 1991
BfuAI ACCTGC 1 cut(s) 1031
BisI GCNGC 7 cut(s) 421, 592, 867, 955, 1319, 1678, 1868
BlpI GCTNAGC 1 cut(s) 1389
BlsI GCNGC 7 cut(s) 422, 593, 868, 956, 1320, 1679, 1869
BmcAI AGTACT 1 cut(s) 1219
Bme1390I CCNGG 8 cut(s) 124, 255, 393, 707, 1070, 1488, 1585, 2026
Bme18I GGWCC 5 cut(s) 7, 824, 1049, 1193, 1981
BmgBI CACGTC 1 cut(s) 1229
BmgT120I GGNCC 7 cut(s) 7, 824, 911, 1049, 1193, 1779, 1981
BmiI GGNNCC 8 cut(s) 29, 390, 1143, 1424, 1530, 1747, 1983, 2030
BmrFI CCNGG 8 cut(s) 124, 255, 393, 707, 1070, 1488, 1585, 2026
BmrI ACTGGG 1 cut(s) 1823
BmsI GCATC 2 cut(s) 376, 1717
BmuI ACTGGG 1 cut(s) 1823
BoxI GACNNNNGTC 2 cut(s) 312, 1738
BpiI GAAGAC 2 cut(s) 177, 236
BpmI CTGGAG 5 cut(s) 237, 831, 951, 1200, 1206
Bpu1102I GCTNAGC 1 cut(s) 1389
BpuEI CTTGAG 3 cut(s) 85, 182, 1875
BsaAI YACGTR 1 cut(s) 312
BsaBI GATNNNNATC 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 678
BsaJI CCNNGG 3 cut(s) 18, 123, 1288
BsaWI WCCGGW 2 cut(s) 273, 2048
BsaXI ACNNNNNCTCC 9 cut(s) 29, 804, 834, 1414, 1444, 1579, 1609, 2020, 2050
Bsc4I CCNNNNNNNGG 5 cut(s) 24, 680, 706, 1043, 1769
Bse1I ACTGG 5 cut(s) 162, 1081, 1196, 1567, 1829
Bse3DI GCAATG 2 cut(s) 1070, 1536
Bse8I GATNNNNATC 1 cut(s) 366
BseAI TCCGGA 1 cut(s) 273
BseBI CCWGG 8 cut(s) 124, 255, 393, 707, 1070, 1488, 1585, 2026
BseDI CCNNGG 3 cut(s) 18, 123, 1288
BseGI GGATG 8 cut(s) 44, 263, 282, 367, 505, 723, 1726, 1954
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 5 cut(s) 24, 680, 706, 1043, 1769
BseMI GCAATG 2 cut(s) 1070, 1536
BseMII CTCAG 3 cut(s) 160, 370, 1746
BseNI ACTGG 5 cut(s) 162, 1081, 1196, 1567, 1829
BseRI GAGGAG 2 cut(s) 203, 401
BseXI GCAGC 6 cut(s) 432, 603, 853, 966, 1330, 1879
BseYI CCCAGC 2 cut(s) 31, 1701
BsgI GTGCAG 1 cut(s) 524
Bsh1236I CGCG 1 cut(s) 895
BshFI GGCC 4 cut(s) 537, 747, 912, 1780
BsiSI CCGG 2 cut(s) 274, 2049
BslFI GGGAC 3 cut(s) 901, 1655, 1967
BslI CCNNNNNNNGG 5 cut(s) 24, 680, 706, 1043, 1769
BsmAI GTCTC 8 cut(s) 72, 185, 300, 482, 678, 1165, 1547, 2077
BsmFI GGGAC 3 cut(s) 901, 1655, 1967
BsmI GAATGC 2 cut(s) 665, 1310
BsnI GGCC 4 cut(s) 537, 747, 912, 1780
Bso31I GGTCTC 1 cut(s) 678
Bsp1286I GDGCHC 1 cut(s) 859
Bsp13I TCCGGA 1 cut(s) 273
Bsp143I GATC 9 cut(s) 261, 562, 937, 1528, 1630, 1745, 1783, 1840, 2054
Bsp1720I GCTNAGC 1 cut(s) 1389
BspACI CCGC 8 cut(s) 5, 128, 349, 689, 1052, 1677, 1844, 1976
BspANI GGCC 4 cut(s) 537, 747, 912, 1780
BspCNI CTCAG 3 cut(s) 159, 369, 1747
BspEI TCCGGA 1 cut(s) 273
BspFNI CGCG 1 cut(s) 895
BspHI TCATGA 1 cut(s) 1330
BspLI GGNNCC 8 cut(s) 29, 390, 1143, 1424, 1530, 1747, 1983, 2030
BspMAI CTGCAG 1 cut(s) 1042
BspMI ACCTGC 1 cut(s) 1031
BspPI GGATC 8 cut(s) 269, 1523, 1536, 1740, 1753, 1778, 1835, 2062
BspQI GCTCTTC 1 cut(s) 1434
BspTI CTTAAG 1 cut(s) 1991
BspTNI GGTCTC 1 cut(s) 678
BsrBI CCGCTC 1 cut(s) 351
BsrDI GCAATG 2 cut(s) 1070, 1536
BsrI ACTGG 5 cut(s) 162, 1081, 1196, 1567, 1829
BssECI CCNNGG 3 cut(s) 18, 123, 1288
BssMI GATC 9 cut(s) 261, 562, 937, 1528, 1630, 1745, 1783, 1840, 2054
BssT1I CCWWGG 1 cut(s) 1288
Bst2UI CCWGG 8 cut(s) 124, 255, 393, 707, 1070, 1488, 1585, 2026
Bst6I CTCTTC 3 cut(s) 70, 1434, 1473
BstAFI CTTAAG 1 cut(s) 1991
BstBAI YACGTR 1 cut(s) 312
BstC8I GCNNGC 3 cut(s) 539, 1446, 1961
BstDEI CTNAG 5 cut(s) 146, 356, 1389, 1755, 1874
BstEII GGTNACC 1 cut(s) 1595
BstF5I GGATG 8 cut(s) 44, 263, 282, 367, 505, 723, 1726, 1954
BstFNI CGCG 1 cut(s) 895
BstH2I RGCGCY 1 cut(s) 2006
BstHHI GCGC 2 cut(s) 897, 2005
BstKTI GATC 9 cut(s) 264, 565, 940, 1531, 1633, 1748, 1786, 1843, 2057
BstMAI GTCTC 8 cut(s) 72, 185, 300, 482, 678, 1165, 1547, 2077
BstMBI GATC 9 cut(s) 261, 562, 937, 1528, 1630, 1745, 1783, 1840, 2054
BstMWI GCNNNNNNNGC 6 cut(s) 147, 781, 863, 1060, 1348, 1431
BstNI CCWGG 8 cut(s) 124, 255, 393, 707, 1070, 1488, 1585, 2026
BstNSI RCATGY 1 cut(s) 1448
BstPAI GACNNNNGTC 2 cut(s) 312, 1738
BstPI GGTNACC 1 cut(s) 1595
BstSCI CCNGG 8 cut(s) 122, 253, 391, 705, 1068, 1486, 1583, 2024
BstSFI CTRYAG 2 cut(s) 234, 1038
BstUI CGCG 1 cut(s) 895
BstV1I GCAGC 6 cut(s) 432, 603, 853, 966, 1330, 1879
BstV2I GAAGAC 2 cut(s) 177, 236
BstX2I RGATCY 2 cut(s) 1528, 1745
BstXI CCANNNNNNTGG 2 cut(s) 39, 1701
BstYI RGATCY 2 cut(s) 1528, 1745
BsuRI GGCC 4 cut(s) 537, 747, 912, 1780
BtgZI GCGATG 2 cut(s) 353, 1605
BtrI CACGTC 1 cut(s) 1229
BtsCI GGATG 8 cut(s) 44, 263, 282, 367, 505, 723, 1726, 1954
BtsI GCAGTG 2 cut(s) 430, 1666
BtsIMutI CAGTG 5 cut(s) 51, 430, 1560, 1666, 2086
BveI ACCTGC 1 cut(s) 1031
Cac8I GCNNGC 3 cut(s) 539, 1446, 1961
CaiI CAGNNNCTG 1 cut(s) 674
CciI TCATGA 1 cut(s) 1330
CfoI GCGC 2 cut(s) 897, 2005
Cfr13I GGNCC 7 cut(s) 7, 824, 911, 1049, 1193, 1779, 1981
CseI GACGC 1 cut(s) 1394
Csp6I GTAC 3 cut(s) 1218, 1262, 1269
CviAII CATG 9 cut(s) 522, 728, 1091, 1331, 1445, 1637, 1776, 1788, 1973
CviQI GTAC 3 cut(s) 1218, 1262, 1269
DdeI CTNAG 5 cut(s) 146, 356, 1389, 1755, 1874
DpnI GATC 9 cut(s) 263, 564, 939, 1530, 1632, 1747, 1785, 1842, 2056
DpnII GATC 9 cut(s) 261, 562, 937, 1528, 1630, 1745, 1783, 1840, 2054
Eam1104I CTCTTC 3 cut(s) 70, 1434, 1473
EarI CTCTTC 3 cut(s) 70, 1434, 1473
EciI GGCGGA 3 cut(s) 20, 143, 1041
Eco130I CCWWGG 1 cut(s) 1288
Eco147I AGGCCT 1 cut(s) 537
Eco24I GRGCYC 1 cut(s) 859
Eco31I GGTCTC 1 cut(s) 678
Eco47I GGWCC 5 cut(s) 7, 824, 1049, 1193, 1981
Eco47III AGCGCT 1 cut(s) 2004
Eco57I CTGAAG 5 cut(s) 192, 588, 1296, 1497, 1590
Eco72I CACGTG 1 cut(s) 312
Eco91I GGTNACC 1 cut(s) 1595
EcoO109I RGGNCCY 1 cut(s) 1981
EcoO65I GGTNACC 1 cut(s) 1595
EcoRII CCWGG 8 cut(s) 122, 253, 391, 705, 1068, 1486, 1583, 2024
EcoT14I CCWWGG 1 cut(s) 1288
EcoT22I ATGCAT 2 cut(s) 665, 1793
EcoT38I GRGCYC 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 1288
FaeI CATG 9 cut(s) 525, 731, 1094, 1334, 1448, 1640, 1779, 1791, 1976
FalI AAGNNNNNCTT 2 cut(s) 1387, 1419
FaqI GGGAC 3 cut(s) 901, 1655, 1967
FatI CATG 9 cut(s) 521, 727, 1090, 1330, 1444, 1636, 1775, 1787, 1972
FauI CCCGC 1 cut(s) 696
FbaI TGATCA 2 cut(s) 562, 937
FblI GTMKAC 1 cut(s) 1833
Fnu4HI GCNGC 7 cut(s) 421, 592, 867, 955, 1319, 1678, 1868
FokI GGATG 8 cut(s) 51, 270, 289, 374, 512, 730, 1733, 1961
FriOI GRGCYC 1 cut(s) 859
Fsp4HI GCNGC 7 cut(s) 421, 592, 867, 955, 1319, 1678, 1868
FspBI CTAG 4 cut(s) 806, 1175, 1302, 1928
GlaI GCGC 2 cut(s) 896, 2004
GluI GCNGC 7 cut(s) 421, 592, 867, 955, 1319, 1678, 1868
GsaI CCCAGC 2 cut(s) 35, 1705
GsuI CTGGAG 5 cut(s) 237, 831, 951, 1200, 1206
HaeII RGCGCY 1 cut(s) 2006
HaeIII GGCC 4 cut(s) 537, 747, 912, 1780
HapII CCGG 2 cut(s) 274, 2049
HgaI GACGC 1 cut(s) 1394
HhaI GCGC 2 cut(s) 897, 2005
Hin1II CATG 9 cut(s) 525, 731, 1094, 1334, 1448, 1640, 1779, 1791, 1976
Hin6I GCGC 2 cut(s) 895, 2003
HinP1I GCGC 2 cut(s) 895, 2003
HinfI GANTC 6 cut(s) 95, 193, 644, 971, 1007, 1555
HpaII CCGG 2 cut(s) 274, 2049
HphI GGTGA 3 cut(s) 653, 1394, 1607
Hpy166II GTNNAC 5 cut(s) 107, 1264, 1834, 2039, 2065
Hpy8I GTNNAC 5 cut(s) 107, 1264, 1834, 2039, 2065
Hpy99I CGWCG 2 cut(s) 864, 1233
HpyCH4IV ACGT 2 cut(s) 311, 1228
HpyF10VI GCNNNNNNNGC 6 cut(s) 147, 781, 863, 1060, 1348, 1431
HpyF3I CTNAG 5 cut(s) 146, 356, 1389, 1755, 1874
HpySE526I ACGT 2 cut(s) 311, 1228
Hsp92II CATG 9 cut(s) 525, 731, 1094, 1334, 1448, 1640, 1779, 1791, 1976
HspAI GCGC 2 cut(s) 895, 2003
Kpn2I TCCGGA 1 cut(s) 273
Ksp22I TGATCA 2 cut(s) 562, 937
Kzo9I GATC 9 cut(s) 261, 562, 937, 1528, 1630, 1745, 1783, 1840, 2054
LguI GCTCTTC 1 cut(s) 1434
LmnI GCTCC 8 cut(s) 33, 331, 388, 676, 1141, 1422, 1587, 2028
Lsp1109I GCAGC 6 cut(s) 432, 603, 853, 966, 1330, 1879
LweI GCATC 2 cut(s) 376, 1717
MaeI CTAG 4 cut(s) 806, 1175, 1302, 1928
MaeII ACGT 2 cut(s) 311, 1228
MaeIII GTNAC 3 cut(s) 524, 1382, 1595
MalI GATC 9 cut(s) 263, 564, 939, 1530, 1632, 1747, 1785, 1842, 2056
MbiI CCGCTC 1 cut(s) 351
MboI GATC 9 cut(s) 261, 562, 937, 1528, 1630, 1745, 1783, 1840, 2054
MboII GAAGA 9 cut(s) 87, 182, 241, 452, 1275, 1451, 1490, 1513, 1596
MflI RGATCY 2 cut(s) 1528, 1745
MhlI GDGCHC 1 cut(s) 859
MluCI AATT 9 cut(s) 183, 434, 458, 514, 584, 1032, 1294, 1374, 1796
MlyI GAGTC 2 cut(s) 187, 1549
MmeI TCCRAC 1 cut(s) 1693
Mph1103I ATGCAT 2 cut(s) 665, 1793
MroI TCCGGA 1 cut(s) 273
MroXI GAANNNNTTC 1 cut(s) 1860
MseI TTAA 7 cut(s) 401, 513, 596, 959, 1242, 1377, 1992
MspA1I CMGCKG 1 cut(s) 35
MspCI CTTAAG 1 cut(s) 1991
MspI CCGG 2 cut(s) 274, 2049
MspR9I CCNGG 8 cut(s) 124, 255, 393, 707, 1070, 1488, 1585, 2026
Mva1269I GAATGC 2 cut(s) 665, 1310
MvaI CCWGG 8 cut(s) 124, 255, 393, 707, 1070, 1488, 1585, 2026
MvnI CGCG 1 cut(s) 895
MwoI GCNNNNNNNGC 6 cut(s) 147, 781, 863, 1060, 1348, 1431
NdeII GATC 9 cut(s) 261, 562, 937, 1528, 1630, 1745, 1783, 1840, 2054
NlaIII CATG 9 cut(s) 525, 731, 1094, 1334, 1448, 1640, 1779, 1791, 1976
NlaIV GGNNCC 8 cut(s) 29, 390, 1143, 1424, 1530, 1747, 1983, 2030
NmeAIII GCCGAG 2 cut(s) 438, 1491
NmuCI GTSAC 2 cut(s) 1382, 1595
NsiI ATGCAT 2 cut(s) 665, 1793
NspI RCATGY 1 cut(s) 1448
PaeI GCATGC 1 cut(s) 1448
PagI TCATGA 1 cut(s) 1330
PaqCI CACCTGC 1 cut(s) 1031
PceI AGGCCT 1 cut(s) 537
PciSI GCTCTTC 1 cut(s) 1434
PcsI WCGNNNNNNNCGW 1 cut(s) 859
PctI GAATGC 2 cut(s) 665, 1310
PdmI GAANNNNTTC 1 cut(s) 1860
PfeI GAWTC 4 cut(s) 95, 644, 971, 1007
PflMI CCANNNNNTGG 1 cut(s) 706
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 7 cut(s) 422, 593, 868, 956, 1320, 1679, 1869
PleI GAGTC 2 cut(s) 187, 1549
PmaCI CACGTG 1 cut(s) 312
PmlI CACGTG 1 cut(s) 312
PpsI GAGTC 2 cut(s) 187, 1549
Ppu21I YACGTR 1 cut(s) 312
PpuMI RGGWCCY 1 cut(s) 1981
PshAI GACNNNNGTC 2 cut(s) 312, 1738
PshBI ATTAAT 1 cut(s) 1377
PsiI TTATAA 1 cut(s) 1470
Psp5II RGGWCCY 1 cut(s) 1981
Psp6I CCWGG 8 cut(s) 122, 253, 391, 705, 1068, 1486, 1583, 2024
PspCI CACGTG 1 cut(s) 312
PspEI GGTNACC 1 cut(s) 1595
PspFI CCCAGC 2 cut(s) 31, 1701
PspGI CCWGG 8 cut(s) 122, 253, 391, 705, 1068, 1486, 1583, 2024
PspN4I GGNNCC 8 cut(s) 29, 390, 1143, 1424, 1530, 1747, 1983, 2030
PspPI GGNCC 7 cut(s) 7, 824, 911, 1049, 1193, 1779, 1981
PspPPI RGGWCCY 1 cut(s) 1981
PsrI GAACNNNNNNTAC 2 cut(s) 883, 915
PstI CTGCAG 1 cut(s) 1042
PstNI CAGNNNCTG 1 cut(s) 674
PsuI RGATCY 2 cut(s) 1528, 1745
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 3 cut(s) 1219, 1263, 1270
RsaNI GTAC 3 cut(s) 1218, 1262, 1269
SapI GCTCTTC 1 cut(s) 1434
SaqAI TTAA 7 cut(s) 401, 513, 596, 959, 1242, 1377, 1992
SatI GCNGC 7 cut(s) 421, 592, 867, 955, 1319, 1678, 1868
Sau3AI GATC 9 cut(s) 261, 562, 937, 1528, 1630, 1745, 1783, 1840, 2054
Sau96I GGNCC 7 cut(s) 7, 824, 911, 1049, 1193, 1779, 1981
SbfI CCTGCAGG 1 cut(s) 1042
ScaI AGTACT 1 cut(s) 1219
SchI GAGTC 2 cut(s) 187, 1549
ScrFI CCNGG 8 cut(s) 124, 255, 393, 707, 1070, 1488, 1585, 2026
SdaI CCTGCAGG 1 cut(s) 1042
SduI GDGCHC 1 cut(s) 859
SfaNI GCATC 2 cut(s) 376, 1717
SfcI CTRYAG 2 cut(s) 234, 1038
SinI GGWCC 5 cut(s) 7, 824, 1049, 1193, 1981
SmlI CTYRAG 4 cut(s) 100, 197, 1890, 1991
SmoI CTYRAG 4 cut(s) 100, 197, 1890, 1991
SphI GCATGC 1 cut(s) 1448
Sse8387I CCTGCAGG 1 cut(s) 1042
Sse9I AATT 9 cut(s) 183, 434, 458, 514, 584, 1032, 1294, 1374, 1796
SseBI AGGCCT 1 cut(s) 537
SsiI CCGC 8 cut(s) 5, 128, 349, 689, 1052, 1677, 1844, 1976
SspMI CTAG 4 cut(s) 806, 1175, 1302, 1928
StuI AGGCCT 1 cut(s) 537
StyD4I CCNGG 8 cut(s) 122, 253, 391, 705, 1068, 1486, 1583, 2024
StyI CCWWGG 1 cut(s) 1288
TaiI ACGT 2 cut(s) 314, 1231
TaqI TCGA 6 cut(s) 408, 853, 862, 1231, 1851, 2017
TasI AATT 9 cut(s) 183, 434, 458, 514, 584, 1032, 1294, 1374, 1796
TatI WGTACW 2 cut(s) 1217, 1261
TauI GCSGC 1 cut(s) 1680
TfiI GAWTC 4 cut(s) 95, 644, 971, 1007
Tru1I TTAA 7 cut(s) 401, 513, 596, 959, 1242, 1377, 1992
Tru9I TTAA 7 cut(s) 401, 513, 596, 959, 1242, 1377, 1992
TscAI CASTG 4 cut(s) 51, 430, 1567, 1666
TseFI GTSAC 2 cut(s) 1382, 1595
TseI GCWGC 6 cut(s) 420, 591, 866, 954, 1318, 1867
Tsp45I GTSAC 2 cut(s) 1382, 1595
TspDTI ATGAA 7 cut(s) 459, 510, 645, 744, 1035, 1107, 1764
TspGWI ACGGA 3 cut(s) 543, 1541, 1961
TspRI CASTG 4 cut(s) 51, 430, 1567, 1666
Van91I CCANNNNNTGG 1 cut(s) 706
Vha464I CTTAAG 1 cut(s) 1991
VpaK11BI GGWCC 5 cut(s) 7, 824, 1049, 1193, 1981
VspI ATTAAT 1 cut(s) 1377
XapI RAATTY 1 cut(s) 1796
XbaI TCTAGA 1 cut(s) 1927
XceI RCATGY 1 cut(s) 1448
XmiI GTMKAC 1 cut(s) 1833
XmnI GAANNNNTTC 1 cut(s) 1860
XspI CTAG 4 cut(s) 806, 1175, 1302, 1928
ZrmI AGTACT 1 cut(s) 1219
Zsp2I ATGCAT 2 cut(s) 665, 1793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.