MD07G1267200.v1.1

methyl-CpG-binding domain-containing protein 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003930
Physical Location & Seq
Forward (+)
33514154 .. 33518469
4316 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1267200.v1.1.491

Sequence Viewer

Length: 2304 bp
ATGTGTCTTTTACTGATAAAAAACGAGGGGTATTATCGTCTAACAATTATAACTCGCAGCCCTAGCAATACAGTATCGGAAACACGTGGGTCCGAAGACCTTCGAAGCTTTTTAGTTTTCAACATTTTACTGAGAGAGAGAGAGAGAGAGTGTGTGTGCGACTCATATTCCAGCGGTGGTCGCCGGGGAAGTCATTTTCCGGCGATGGAGGAGCAGAACTCCGAGGACTGGCTCCCAACTGGATGGACAGTGGAGGTCAAGTTTAGAAAAAACGGCAAAAGAGACAAGTATTATTACGAATCCTCAAGTGGGCATAGATTTAGTTCCAGGGCAGAGGTATCTCGATATCTCAGCACCTCCAGTCATGAAGACGAACAGAAAATTAAAGAGACTCTCAAGCGACCTTCAGATGATGTTGTAGTTGAGAAGACTGTAGCAGAAGGACTACCTCCAGGATGGACCAAAGAAATTCGGATTACAAAAAAGGCTCGTAAGACTAGGAGAGACACGCATTATATTGCTCCTGCAAGTGAAAAAGTATTCTGCTCTATCAGGGATGTGCATCGCTATCTTGAAAATGAGCAGCCAGGAACGTTAACTCTTGAGCCGAGTGGTAGCGGCGATAAGGAATCGGAAGATCAAAAAGCGTTTGAATTAGACAAACCGAGCTTGAAATTGAATGAGACAATGAAGGATGGAGAGATACTTAATTGTTCATGTAATAGAGAAGGCCCGTCCCTTCCTGAACATACTTCTGATCAGAGTGAGGTGGGCACTGAATTAGGCAGCTTAAATCCTTCAGAAGCCAAAGGTTTGGAGCAAAATGAACAGGATGGTGATTCCACAAAAAGTGAATGTATTTCAGCTCCTGCAGGTGGAGACCCACCAGGTAGCCAATGCCTGGAAAGTGGGCTGATAAAACATGAAAGAAACCGTCCTGGTCTCTGCAAATCAAAGAACAAGAAAGAGCTTATCTTGCCTCGTCGTGCTTCAAAGCGACTAGCTGGAGTTGAAGTTGGTCCAGTTCCAGAACTGAAAACAAGTACTCGAACCCGTCGAGTTGCAATTGAACAGTCAGGGGAGGGAGCACGCCATACAACTGAAGGCCCGTCCCTTCCTGAACACAGTTCTGATCAGAGTGAGGTGGGCACTGAATCAGGCAGCTTAAATCCTCCAGAAGCCAAAGGTTCGGACCAAACAGAACAGGACAGTGATTCCACAAATGGTGAATGTATTTCAGTTGCTGCAGGTGGAGACCTGCCAGGTAAGCAATGCCTGGAAAGTGGGCTGATAAAACATGAAAGGAAAAAGACCCGTCTTGGCCTTTGCAAATCAAAGAACAAGATAGAGCTTATGTTGCCTCGCCGTGCTTCAAAGCGACTAGCTGGAGTTGAAGTTGGTCCGGTTCCAGAACTGAAAACAAGTACTCGAGCATGTCGAGTTGCAATTAAACAGTCAGGGGAGGGAGTCCGCCATACAACTGAAGGTTCTTCCCCTGGTGATTTGCTCAGTTGTGCGTCCCAACATCCTGATTATCTTGAGGTTGAGCCAGAAAAGCATATTCAAAATGTGGAACGAGCAATTAATGGTGACAACAAGCAACGGTGTGCTTCTGTTTTCCCATCGGGGAGCCAAAGTGGCCCAGAAGAGCATGCAGGAAAACTTGAAACCGATTATAAGGCTGAAGATAAACCAGGACTTCAACCAGACTTGCCTCTTGGGGATTTCTTCACAGATCCCTGCATTGCATTTGCAATAGAGACTCTCACTGGAATAGGTTTTGACAATTCCAGGAGCAATGGTGAGCATGCTTCAGGGGACCTGGCTACTCCAAAAGGTCATCCTGGGGACGTACAAGCAGACAATAAGAGCAGCAGAAAGCAGGAAAGCGGCAACCTTTTTATACCAGAAGATGGATGGAAAGTTGCAAAGGACGATAACGCTAGACAGAAGTCCGGATCCCCAACTGAGTTGCCTTTTGGGGGTTCATGGCCCGACCCGTGCATTGAATTTGCTATTAAAACTCTCACGGGTGCAATCCCAGTAGACTACGATCCCCATATCGAGGAGTACTTTCAGCAGCAACTAAGCTCATCAAAAACTCAAGGAACAAGTGACTTGACCTTGAAAAATGTTGGTCTAGATAACTTCTGCCAAACGGATGATTTGTGCGAGCAATTCTGTCATCCGGAGGTCCCCATGCTTAAGAAACAAGCGCTTTCTGAGCCTCGACTCACCAAGAGTGGAAGTTTACATAGTTCCGGTGGATCAGATGTAAACCAAACGGGGAGGCAGAGACACTGA

Protein Analysis

768

Amino Acids

84.15

Weight (kDa)

5.75

Isoelectric Point (pI)

58.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 73 - 125 1.2e-07 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 50, 1677
AarI CACCTGC 2 cut(s) 863, 1238
Acc36I ACCTGC 3 cut(s) 863, 1238, 1266
AccB7I CCANNNNNTGG 2 cut(s) 901, 1913
AccI GTMKAC 1 cut(s) 2046
AccIII TCCGGA 2 cut(s) 1955, 2188
AciI CCGC 4 cut(s) 174, 618, 1471, 1890
AclI AACGTT 1 cut(s) 593
AclWI GGATC 5 cut(s) 1730, 1953, 1966, 2048, 2275
AcsI RAATTY 2 cut(s) 468, 2009
AcuI CTGAAG 6 cut(s) 390, 783, 1122, 1503, 1704, 1797
AcvI CACGTG 1 cut(s) 86
AfaI GTAC 4 cut(s) 1045, 1426, 1854, 2072
AfeI AGCGCT 1 cut(s) 2217
AfiI CCNNNNNNNGG 7 cut(s) 228, 309, 875, 901, 1913, 1982, 2065
AflII CTTAAG 1 cut(s) 2204
AflIII ACRYGT 1 cut(s) 83
Alw21I GWGCWC 1 cut(s) 1090
Alw26I GTCTC 9 cut(s) 276, 383, 498, 677, 873, 947, 1248, 1754, 2290
AlwI GGATC 5 cut(s) 1730, 1953, 1966, 2048, 2275
AlwNI CAGNNNCTG 2 cut(s) 869, 1244
Ama87I CYCGRG 1 cut(s) 1428
Aor13HI TCCGGA 2 cut(s) 1955, 2188
Aor51HI AGCGCT 1 cut(s) 2217
AoxI GGCC 5 cut(s) 730, 1105, 1321, 1639, 1991
ApeKI GCWGC 7 cut(s) 57, 583, 786, 1161, 1244, 1872, 2080
ApoI RAATTY 2 cut(s) 468, 2009
AseI ATTAAT 1 cut(s) 1584
Asp700I GAANNNNTTC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 2218
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 6 cut(s) 848, 1238, 1511, 1601, 1814, 2227
AsuII TTCGAA 1 cut(s) 103
AvaI CYCGRG 1 cut(s) 1428
AvaII GGWCC 7 cut(s) 90, 459, 1019, 1192, 1400, 1819, 2194
BaeGI GKGCMC 2 cut(s) 776, 1151
BamHI GGATCC 1 cut(s) 1958
BbrPI CACGTG 1 cut(s) 86
BbsI GAAGAC 3 cut(s) 102, 375, 434
Bbv12I GWGCWC 1 cut(s) 1090
BbvI GCAGC 7 cut(s) 69, 595, 798, 1173, 1231, 1884, 2092
BccI CCATC 8 cut(s) 199, 237, 450, 689, 827, 1630, 1907, 1911
BceAI ACGGC 2 cut(s) 289, 1350
BclI TGATCA 2 cut(s) 757, 1132
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 9 cut(s) 276, 383, 498, 677, 873, 947, 1248, 1754, 2290
BfaI CTAG 6 cut(s) 63, 498, 1001, 1382, 1944, 2141
BfmI CTRYAG 3 cut(s) 432, 870, 1245
BfoI RGCGCY 1 cut(s) 2219
BfrI CTTAAG 1 cut(s) 2204
BfuAI ACCTGC 3 cut(s) 863, 1238, 1266
BglI GCCNNNNNGGC 1 cut(s) 1638
BisI GCNGC 9 cut(s) 58, 584, 619, 787, 1162, 1245, 1873, 1891, 2081
BlsI GCNGC 9 cut(s) 59, 585, 620, 788, 1163, 1246, 1874, 1892, 2082
BmcAI AGTACT 3 cut(s) 1045, 1426, 2072
Bme18I GGWCC 7 cut(s) 90, 459, 1019, 1192, 1400, 1819, 2194
BmeT110I CYCGRG 1 cut(s) 1428
BmiI GGNNCC 7 cut(s) 91, 233, 1407, 1631, 1820, 1960, 2196
BmrI ACTGGG 1 cut(s) 2036
BmsI GCATC 1 cut(s) 571
BmuI ACTGGG 1 cut(s) 2036
BoxI GACNNNNGTC 1 cut(s) 1951
BpiI GAAGAC 3 cut(s) 102, 375, 434
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 5 cut(s) 343, 435, 1026, 1158, 1407
Bpu14I TTCGAA 1 cut(s) 103
BpuEI CTTGAG 5 cut(s) 289, 380, 623, 1559, 2088
BpuMI CCSGG 1 cut(s) 185
BsaAI YACGTR 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 561
BsaI GGTCTC 3 cut(s) 873, 947, 1248
BsaJI CCNNGG 5 cut(s) 184, 222, 327, 1495, 1844
BsaWI WCCGGW 4 cut(s) 1402, 1955, 2188, 2261
Bsc4I CCNNNNNNNGG 7 cut(s) 228, 309, 875, 901, 1913, 1982, 2065
Bse1I ACTGG 6 cut(s) 233, 244, 360, 1022, 1774, 2042
Bse3DI GCAATG 3 cut(s) 1277, 1743, 1804
Bse8I GATNNNNATC 1 cut(s) 561
BseAI TCCGGA 2 cut(s) 1955, 2188
BseDI CCNNGG 5 cut(s) 184, 222, 327, 1495, 1844
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 7 cut(s) 228, 309, 875, 901, 1913, 1982, 2065
BseMI GCAATG 3 cut(s) 1277, 1743, 1804
BseMII CTCAG 5 cut(s) 122, 364, 1522, 1959, 2214
BseNI ACTGG 6 cut(s) 233, 244, 360, 1022, 1774, 2042
BseRI GAGGAG 2 cut(s) 224, 2081
BseSI GKGCMC 2 cut(s) 776, 1151
BseXI GCAGC 7 cut(s) 69, 595, 798, 1173, 1231, 1884, 2092
BshFI GGCC 5 cut(s) 732, 1107, 1323, 1641, 1993
BsiHKAI GWGCWC 1 cut(s) 1090
BsiHKCI CYCGRG 1 cut(s) 1428
BsiSI CCGG 6 cut(s) 184, 200, 1403, 1956, 2189, 2262
BslFI GGGAC 6 cut(s) 721, 1096, 1504, 1832, 1862, 2180
BslI CCNNNNNNNGG 7 cut(s) 228, 309, 875, 901, 1913, 1982, 2065
BsmAI GTCTC 9 cut(s) 276, 383, 498, 677, 873, 947, 1248, 1754, 2290
BsmFI GGGAC 6 cut(s) 721, 1096, 1504, 1832, 1862, 2180
BsnI GGCC 5 cut(s) 732, 1107, 1323, 1641, 1993
Bso31I GGTCTC 3 cut(s) 873, 947, 1248
BsoBI CYCGRG 1 cut(s) 1428
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 3 cut(s) 776, 1090, 1151
Bsp13I TCCGGA 2 cut(s) 1955, 2188
Bsp143I GATC 7 cut(s) 637, 757, 1132, 1735, 1958, 2053, 2267
BspACI CCGC 4 cut(s) 174, 618, 1471, 1890
BspANI GGCC 5 cut(s) 732, 1107, 1323, 1641, 1993
BspCNI CTCAG 5 cut(s) 123, 363, 1521, 1960, 2215
BspEI TCCGGA 2 cut(s) 1955, 2188
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 7 cut(s) 91, 233, 1407, 1631, 1820, 1960, 2196
BspMAI CTGCAG 2 cut(s) 874, 1249
BspMI ACCTGC 3 cut(s) 863, 1238, 1266
BspPI GGATC 5 cut(s) 1730, 1953, 1966, 2048, 2275
BspQI GCTCTTC 1 cut(s) 1641
BspT104I TTCGAA 1 cut(s) 103
BspTI CTTAAG 1 cut(s) 2204
BspTNI GGTCTC 3 cut(s) 873, 947, 1248
BsrDI GCAATG 3 cut(s) 1277, 1743, 1804
BsrI ACTGG 6 cut(s) 233, 244, 360, 1022, 1774, 2042
BssECI CCNNGG 5 cut(s) 184, 222, 327, 1495, 1844
BssMI GATC 7 cut(s) 637, 757, 1132, 1735, 1958, 2053, 2267
Bst4CI ACNGT 9 cut(s) 73, 250, 433, 935, 1074, 1127, 1211, 1455, 1605
Bst6I CTCTTC 1 cut(s) 1641
BstAFI CTTAAG 1 cut(s) 2204
BstBAI YACGTR 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 4 cut(s) 1090, 1653, 1809, 2174
BstDEI CTNAG 6 cut(s) 131, 350, 1508, 1968, 2087, 2223
BstH2I RGCGCY 1 cut(s) 2219
BstHHI GCGC 1 cut(s) 2218
BstKTI GATC 7 cut(s) 640, 760, 1135, 1738, 1961, 2056, 2270
BstMAI GTCTC 9 cut(s) 276, 383, 498, 677, 873, 947, 1248, 1754, 2290
BstMBI GATC 7 cut(s) 637, 757, 1132, 1735, 1958, 2053, 2267
BstMWI GCNNNNNNNGC 8 cut(s) 63, 180, 976, 1267, 1357, 1555, 1638, 2224
BstNSI RCATGY 3 cut(s) 1437, 1655, 1811
BstPAI GACNNNNGTC 1 cut(s) 1951
BstSFI CTRYAG 3 cut(s) 432, 870, 1245
BstSLI GKGCMC 2 cut(s) 776, 1151
BstV1I GCAGC 7 cut(s) 69, 595, 798, 1173, 1231, 1884, 2092
BstV2I GAAGAC 3 cut(s) 102, 375, 434
BstX2I RGATCY 2 cut(s) 1735, 1958
BstXI CCANNNNNNTGG 2 cut(s) 243, 814
BstYI RGATCY 2 cut(s) 1735, 1958
BsuRI GGCC 5 cut(s) 732, 1107, 1323, 1641, 1993
BtgZI GCGATG 2 cut(s) 218, 548
BtsIMutI CAGTG 6 cut(s) 255, 774, 1149, 1216, 1767, 2299
BveI ACCTGC 3 cut(s) 863, 1238, 1266
Cac8I GCNNGC 4 cut(s) 1090, 1653, 1809, 2174
CaiI CAGNNNCTG 2 cut(s) 869, 1244
CciI TCATGA 1 cut(s) 364
CfoI GCGC 1 cut(s) 2218
CseI GACGC 1 cut(s) 1506
CsiI ACCWGGT 1 cut(s) 886
Csp6I GTAC 4 cut(s) 1044, 1425, 1853, 2071
CviAII CATG 9 cut(s) 365, 717, 923, 1298, 1434, 1652, 1808, 1989, 2200
CviQI GTAC 4 cut(s) 1044, 1425, 1853, 2071
DdeI CTNAG 6 cut(s) 131, 350, 1508, 1968, 2087, 2223
DpnI GATC 7 cut(s) 639, 759, 1134, 1737, 1960, 2055, 2269
DpnII GATC 7 cut(s) 637, 757, 1132, 1735, 1958, 2053, 2267
Eam1104I CTCTTC 1 cut(s) 1641
EarI CTCTTC 1 cut(s) 1641
EciI GGCGGA 1 cut(s) 1460
Eco31I GGTCTC 3 cut(s) 873, 947, 1248
Eco32I GATATC 1 cut(s) 347
Eco47I GGWCC 7 cut(s) 90, 459, 1019, 1192, 1400, 1819, 2194
Eco47III AGCGCT 1 cut(s) 2217
Eco57I CTGAAG 6 cut(s) 390, 783, 1122, 1503, 1704, 1797
Eco72I CACGTG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 1428
EcoO109I RGGNCCY 2 cut(s) 1819, 2194
EcoRV GATATC 1 cut(s) 347
FaeI CATG 9 cut(s) 368, 720, 926, 1301, 1437, 1655, 1811, 1992, 2203
FaqI GGGAC 6 cut(s) 721, 1096, 1504, 1832, 1862, 2180
FatI CATG 9 cut(s) 364, 716, 922, 1297, 1433, 1651, 1807, 1988, 2199
FbaI TGATCA 2 cut(s) 757, 1132
FblI GTMKAC 1 cut(s) 2046
Fnu4HI GCNGC 9 cut(s) 58, 584, 619, 787, 1162, 1245, 1873, 1891, 2081
Fsp4HI GCNGC 9 cut(s) 58, 584, 619, 787, 1162, 1245, 1873, 1891, 2081
FspBI CTAG 6 cut(s) 63, 498, 1001, 1382, 1944, 2141
GlaI GCGC 1 cut(s) 2217
GluI GCNGC 9 cut(s) 58, 584, 619, 787, 1162, 1245, 1873, 1891, 2081
GsuI CTGGAG 5 cut(s) 343, 435, 1026, 1158, 1407
HaeII RGCGCY 1 cut(s) 2219
HaeIII GGCC 5 cut(s) 732, 1107, 1323, 1641, 1993
HapII CCGG 6 cut(s) 184, 200, 1403, 1956, 2189, 2262
HgaI GACGC 1 cut(s) 1506
HhaI GCGC 1 cut(s) 2218
Hin1II CATG 9 cut(s) 368, 720, 926, 1301, 1437, 1655, 1811, 1992, 2203
Hin6I GCGC 1 cut(s) 2216
HinP1I GCGC 1 cut(s) 2216
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HindIII AAGCTT 1 cut(s) 106
HpaI GTTAAC 1 cut(s) 597
HpaII CCGG 6 cut(s) 184, 200, 1403, 1956, 2189, 2262
HphI GGTGA 6 cut(s) 848, 1238, 1511, 1601, 1814, 2227
Hpy166II GTNNAC 4 cut(s) 597, 2047, 2252, 2278
Hpy8I GTNNAC 4 cut(s) 597, 2047, 2252, 2278
Hpy99I CGWCG 2 cut(s) 987, 1059
HpyCH4III ACNGT 9 cut(s) 73, 250, 433, 935, 1074, 1127, 1211, 1455, 1605
HpyCH4IV ACGT 3 cut(s) 85, 593, 1851
HpyF10VI GCNNNNNNNGC 8 cut(s) 63, 180, 976, 1267, 1357, 1555, 1638, 2224
HpyF3I CTNAG 6 cut(s) 131, 350, 1508, 1968, 2087, 2223
HpySE526I ACGT 3 cut(s) 85, 593, 1851
Hsp92II CATG 9 cut(s) 368, 720, 926, 1301, 1437, 1655, 1811, 1992, 2203
HspAI GCGC 1 cut(s) 2216
Kpn2I TCCGGA 2 cut(s) 1955, 2188
Ksp22I TGATCA 2 cut(s) 757, 1132
KspAI GTTAAC 1 cut(s) 597
Kzo9I GATC 7 cut(s) 637, 757, 1132, 1735, 1958, 2053, 2267
LguI GCTCTTC 1 cut(s) 1641
LmnI GCTCC 8 cut(s) 211, 237, 526, 817, 871, 1085, 1629, 1794
Lsp1109I GCAGC 7 cut(s) 69, 595, 798, 1173, 1231, 1884, 2092
LweI GCATC 1 cut(s) 571
MabI ACCWGGT 1 cut(s) 886
MaeI CTAG 6 cut(s) 63, 498, 1001, 1382, 1944, 2141
MaeII ACGT 3 cut(s) 85, 593, 1851
MaeIII GTNAC 2 cut(s) 1589, 2114
MalI GATC 7 cut(s) 639, 759, 1134, 1737, 1960, 2055, 2269
MboI GATC 7 cut(s) 637, 757, 1132, 1735, 1958, 2053, 2267
MboII GAAGA 9 cut(s) 107, 380, 439, 647, 1482, 1658, 1697, 1720, 1922
MfeI CAATTG 1 cut(s) 1065
MflI RGATCY 2 cut(s) 1735, 1958
MhlI GDGCHC 3 cut(s) 776, 1090, 1151
MlyI GAGTC 5 cut(s) 155, 385, 1476, 1756, 2226
MroI TCCGGA 2 cut(s) 1955, 2188
MroXI GAANNNNTTC 1 cut(s) 99
MseI TTAA 9 cut(s) 384, 596, 708, 791, 1166, 1449, 1584, 2019, 2205
MspA1I CMGCKG 1 cut(s) 174
MspCI CTTAAG 1 cut(s) 2204
MspI CCGG 6 cut(s) 184, 200, 1403, 1956, 2189, 2262
MunI CAATTG 1 cut(s) 1065
MwoI GCNNNNNNNGC 8 cut(s) 63, 180, 976, 1267, 1357, 1555, 1638, 2224
NciI CCSGG 1 cut(s) 185
NdeII GATC 7 cut(s) 637, 757, 1132, 1735, 1958, 2053, 2267
NlaIII CATG 9 cut(s) 368, 720, 926, 1301, 1437, 1655, 1811, 1992, 2203
NlaIV GGNNCC 7 cut(s) 91, 233, 1407, 1631, 1820, 1960, 2196
NmeAIII GCCGAG 1 cut(s) 633
NmuCI GTSAC 2 cut(s) 1589, 2114
NspI RCATGY 3 cut(s) 1437, 1655, 1811
NspV TTCGAA 1 cut(s) 103
PaeI GCATGC 2 cut(s) 1655, 1811
PaeR7I CTCGAG 1 cut(s) 1428
PagI TCATGA 1 cut(s) 364
PaqCI CACCTGC 2 cut(s) 863, 1238
PciSI GCTCTTC 1 cut(s) 1641
PcsI WCGNNNNNNNCGW 2 cut(s) 1054, 1435
PdmI GAANNNNTTC 1 cut(s) 99
PfeI GAWTC 5 cut(s) 299, 629, 839, 1154, 1214
PflMI CCANNNNNTGG 2 cut(s) 901, 1913
PfoI TCCNGGA 2 cut(s) 451, 1790
PkrI GCNGC 9 cut(s) 59, 585, 620, 788, 1163, 1246, 1874, 1892, 2082
PleI GAGTC 5 cut(s) 155, 385, 1475, 1756, 2226
PmaCI CACGTG 1 cut(s) 86
PmlI CACGTG 1 cut(s) 86
PpsI GAGTC 5 cut(s) 155, 385, 1475, 1756, 2226
Ppu21I YACGTR 1 cut(s) 86
PpuMI RGGWCCY 2 cut(s) 1819, 2194
PshAI GACNNNNGTC 1 cut(s) 1951
PshBI ATTAAT 1 cut(s) 1584
PsiI TTATAA 2 cut(s) 50, 1677
Psp1406I AACGTT 1 cut(s) 593
Psp5II RGGWCCY 2 cut(s) 1819, 2194
PspCI CACGTG 1 cut(s) 86
PspN4I GGNNCC 7 cut(s) 91, 233, 1407, 1631, 1820, 1960, 2196
PspPPI RGGWCCY 2 cut(s) 1819, 2194
PspXI VCTCGAGB 1 cut(s) 1428
PstI CTGCAG 2 cut(s) 874, 1249
PstNI CAGNNNCTG 2 cut(s) 869, 1244
PsuI RGATCY 2 cut(s) 1735, 1958
RsaI GTAC 4 cut(s) 1045, 1426, 1854, 2072
RsaNI GTAC 4 cut(s) 1044, 1425, 1853, 2071
SapI GCTCTTC 1 cut(s) 1641
SaqAI TTAA 9 cut(s) 384, 596, 708, 791, 1166, 1449, 1584, 2019, 2205
SatI GCNGC 9 cut(s) 58, 584, 619, 787, 1162, 1245, 1873, 1891, 2081
Sau3AI GATC 7 cut(s) 637, 757, 1132, 1735, 1958, 2053, 2267
SbfI CCTGCAGG 1 cut(s) 874
ScaI AGTACT 3 cut(s) 1045, 1426, 2072
SchI GAGTC 5 cut(s) 155, 385, 1476, 1756, 2226
SdaI CCTGCAGG 1 cut(s) 874
SduI GDGCHC 3 cut(s) 776, 1090, 1151
SexAI ACCWGGT 1 cut(s) 886
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 3 cut(s) 432, 870, 1245
Sfr274I CTCGAG 1 cut(s) 1428
SfuI TTCGAA 1 cut(s) 103
SinI GGWCC 7 cut(s) 90, 459, 1019, 1192, 1400, 1819, 2194
SlaI CTCGAG 1 cut(s) 1428
SmlI CTYRAG 7 cut(s) 304, 395, 602, 1428, 1538, 2103, 2204
SmoI CTYRAG 7 cut(s) 304, 395, 602, 1428, 1538, 2103, 2204
SphI GCATGC 2 cut(s) 1655, 1811
Sse8387I CCTGCAGG 1 cut(s) 874
SsiI CCGC 4 cut(s) 174, 618, 1471, 1890
SspMI CTAG 6 cut(s) 63, 498, 1001, 1382, 1944, 2141
TaaI ACNGT 9 cut(s) 73, 250, 433, 935, 1074, 1127, 1211, 1455, 1605
TaiI ACGT 3 cut(s) 88, 596, 1854
TaqI TCGA 8 cut(s) 103, 343, 1048, 1057, 1429, 1438, 2064, 2230
TatI WGTACW 3 cut(s) 1043, 1424, 2070
TauI GCSGC 2 cut(s) 621, 1893
TfiI GAWTC 5 cut(s) 299, 629, 839, 1154, 1214
Tru1I TTAA 9 cut(s) 384, 596, 708, 791, 1166, 1449, 1584, 2019, 2205
Tru9I TTAA 9 cut(s) 384, 596, 708, 791, 1166, 1449, 1584, 2019, 2205
TscAI CASTG 5 cut(s) 255, 781, 1156, 1216, 1774
TseFI GTSAC 2 cut(s) 1589, 2114
TseI GCWGC 7 cut(s) 57, 583, 786, 1161, 1244, 1872, 2080
Tsp45I GTSAC 2 cut(s) 1589, 2114
TspDTI ATGAA 7 cut(s) 381, 704, 705, 840, 939, 1314, 1977
TspGWI ACGGA 1 cut(s) 2174
TspRI CASTG 5 cut(s) 255, 781, 1156, 1216, 1774
Van91I CCANNNNNTGG 2 cut(s) 901, 1913
Vha464I CTTAAG 1 cut(s) 2204
VpaK11BI GGWCC 7 cut(s) 90, 459, 1019, 1192, 1400, 1819, 2194
VspI ATTAAT 1 cut(s) 1584
XapI RAATTY 2 cut(s) 468, 2009
XbaI TCTAGA 1 cut(s) 2140
XceI RCATGY 3 cut(s) 1437, 1655, 1811
XcmI CCANNNNNNNNNTGG 1 cut(s) 1914
XhoI CTCGAG 1 cut(s) 1428
XmiI GTMKAC 1 cut(s) 2046
XmnI GAANNNNTTC 1 cut(s) 99
XspI CTAG 6 cut(s) 63, 498, 1001, 1382, 1944, 2141
ZrmI AGTACT 3 cut(s) 1045, 1426, 2072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.