Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0017368

Physical Location & Seq

Genomic Coordinates Reverse (-)

30308741 .. 30310748

Gene Structure 2008 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1208500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGGGCACTGTTGAAGTGGCCGAAGACGATTGGTTGATTTGTTGGGAAGAAAATCTGGTACCAAATCCTTGCAGATTTTTCTCGCTGCTCCCGCTGGTTAGTTGGTACCTGAAAATATTTGAATTTTACCCAAGTGATTTGGATGATCCCAACTTCCTGTGTCAAATCTCTTTTTACTGCTATTTACCAAGCTCCATCTCCATTTGCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGAATATGTCCTCCAGTGCCTTGTCTCTCAAGAAAAAGCTCTTGAAGCCGACCTCATTTTTCGGTGTCAAATCATGGGTTTTGCTTGTAATCTTCATAGCTCTATTCACAGTTATCACTCTCATCATCTCTCTGCTCTGTGTCATCCATTACCGTCGACGAAAATCCTCCAAATCCAACTTCCGTCTCCCGACTCCCATTGTTTCCAAAACTACACACAATGGTTATTGTAATTCATCCCTGGACCGGAGGCTGCTTTCGCGTAACATGTCTGATATTGAGATGAACAGCGGGAAGCTGCAGCAGCACGGGCGTGTGTTTTCCGGTCAGTTCGCATCTCCTCAGGCTAGTGGGAGAATCCGAAATAGTAATGTGATGGAGTTGGGACCGGTTTCTATGTATTCACAAATGGTTCAGGATGTGTGGAGGGGCAAAAAGTTTAGTCTCAAGGAGCTTGAGGTTGCAACGAATGGATTTTCGAAACAAAATCTGATTGGAAATGGGGACTATGGAATTGTTTATCATGGCATTCTGTTCGATAATACGCTCGTGGCAGTGAAAAGGCTAGTGAGTGACAGGTTGCTTGTGCATGAGTATGTAGATAATAAAAGTCTGCACCACTGGCTTCACGAATGCTCGAAACTAGTCAGCCCTCTAACGTGGAGCATTCGGTTGAAAATAATCCGAGGAATCGCAAAAGGATTGGCCTACCTGCACGAGGATATAGAACCGAAAATCCTACACAGAAGCCTAATATCTAGCAATGTGCTGCTCGATCACCAATGGGATCCTAAGGTCTCGGATTTTGGCCTAGCTAAGCTCTACAATCCCGAGTGGGGAATCACTATAATGGAATCATTAGGGTTAGTTTCTTTGCTCTTGGAAAAATCTTTCAGTTACATTGCTCCGGAATATGCTTCTACCGGTGAATTCACTGAGAAGTCCGATATTTATAGCTTTGGAGTACTCGTAATGGAGATCATAACGGGGAGGAGACCTATAGATCGCAGTCAACCCCAACCATATGTGGTTGAATGGTTAAAGTCCATGGTTGCGGGACAGAAAATTGCTTGCGTGGTTGACCCTAAAATGCCAGAGAAGCCATCTCCGAAGGAGCTCAAAAGGGTACTTTTGGTTGCTCTTCGTTGTGTTGATCCCGATTCAGAACATAGGCCTCAAATGGGAGAAGTTATTCACATGCTTGAGCTCCGTGACTTGATGCTCTGCGATTCAAGAACAGGTATTTCCTTTCAATTAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

499

Amino Acids

56.58

Weight (kDa)

8.62

Isoelectric Point (pI)

39.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	271 - 478	1.5e-26	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	271 - 477	1.7e-23	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017368)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g30240
malus_domestica	MD01G1208500.v1.1
prunus_persica	Prupe.2G301100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0378371
rosa_laevigata	RLG00000026440
rosa_multiflora	Rmu_sc0013545.1_g000010
rosa_roxburghii	Rroxscaffold_4G00280500
rosa_rugosa	Rorug01G0408300
rosa_samantha	Rh1AG429800 Rh1BG387500 Rh1CG401000 Rh1DG418600
rosa_wichuraiana	Rw1G037610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	954
Acc65I	GGTACC	2 cut(s)	58, 105
AccB1I	GGYRCC	2 cut(s)	58, 105
AccI	GTMKAC	1 cut(s)	391
AccII	CGCG	1 cut(s)	496
AccIII	TCCGGA	1 cut(s)	1141
AciI	CCGC	3 cut(s)	92, 525, 1289
AclWI	GGATC	4 cut(s)	140, 1016, 1029, 1382
AcoI	YGGCCR	1 cut(s)	18
AcsI	RAATTY	2 cut(s)	122, 1163
AfaI	GTAC	4 cut(s)	60, 107, 1200, 1362
AfiI	CCNNNNNNNGG	4 cut(s)	480, 952, 1070, 1415
AflIII	ACRYGT	1 cut(s)	501
AgeI	ACCGGT	2 cut(s)	622, 1157
AgsI	TTSAA	7 cut(s)	14, 122, 280, 910, 1268, 1467, 1487
AhlI	ACTAGT	1 cut(s)	877
AjnI	CCWGG	1 cut(s)	474
AjuI	GAANNNNNNNTTGG	2 cut(s)	398, 430
AleI	CACNNNNGTG	1 cut(s)	546
AloI	GAACNNNNNNTCC	2 cut(s)	954, 986
Alw21I	GWGCWC	2 cut(s)	1353, 1443
Alw26I	GTCTC	5 cut(s)	264, 425, 683, 1036, 1222
AlwI	GGATC	4 cut(s)	140, 1016, 1029, 1382
Ama87I	CYCGRG	1 cut(s)	1064
Aor13HI	TCCGGA	1 cut(s)	1141
AoxI	GGCC	4 cut(s)	18, 939, 1042, 1406
ApeKI	GCWGC	6 cut(s)	85, 487, 532, 535, 538, 1003
ApoI	RAATTY	2 cut(s)	122, 1163
AsiGI	ACCGGT	2 cut(s)	622, 1157
Asp700I	GAANNNNTTC	1 cut(s)	1425
Asp718I	GGTACC	2 cut(s)	58, 105
AspS9I	GGNCC	2 cut(s)	478, 620
AsuHPI	GGTGA	2 cut(s)	1004, 1172
AsuII	TTCGAA	1 cut(s)	713
AvaI	CYCGRG	1 cut(s)	1064
AvaII	GGWCC	2 cut(s)	478, 620
AxyI	CCTNAGG	2 cut(s)	576, 1026
BaeGI	GKGCMC	1 cut(s)	8
BamHI	GGATCC	1 cut(s)	1021
BanI	GGYRCC	2 cut(s)	58, 105
BanII	GRGCYC	2 cut(s)	1353, 1443
BauI	CACGAG	2 cut(s)	782, 950
BbsI	GAAGAC	1 cut(s)	30
Bbv12I	GWGCWC	2 cut(s)	1353, 1443
BbvI	GCAGC	6 cut(s)	72, 474, 519, 547, 550, 990
BccI	CCATC	3 cut(s)	203, 604, 1345
BcgI	CGANNNNNNTGC	2 cut(s)	751, 785
BciT130I	CCWGG	1 cut(s)	476
BcoDI	GTCTC	5 cut(s)	264, 425, 683, 1036, 1222
BcuI	ACTAGT	1 cut(s)	877
BfaI	CTAG	5 cut(s)	582, 800, 878, 993, 1046
BfmI	CTRYAG	2 cut(s)	533, 1233
BfuAI	ACCTGC	1 cut(s)	954
BisI	GCNGC	6 cut(s)	86, 488, 533, 536, 539, 1004
BlpI	GCTNAGC	1 cut(s)	1050
BlsI	GCNGC	6 cut(s)	87, 489, 534, 537, 540, 1005
BmcAI	AGTACT	1 cut(s)	1200
Bme1390I	CCNGG	1 cut(s)	476
Bme18I	GGWCC	2 cut(s)	478, 620
BmeT110I	CYCGRG	1 cut(s)	1064
BmgT120I	GGNCC	2 cut(s)	478, 620
BmiI	GGNNCC	4 cut(s)	60, 107, 621, 1023
BmrFI	CCNGG	1 cut(s)	476
BmsI	GCATC	2 cut(s)	578, 1443
BpiI	GAAGAC	1 cut(s)	30
BpmI	CTGGAG	1 cut(s)	232
Bpu1102I	GCTNAGC	1 cut(s)	1050
Bpu14I	TTCGAA	1 cut(s)	713
BpuEI	CTTGAG	4 cut(s)	248, 665, 710, 1457
BsaI	GGTCTC	2 cut(s)	1036, 1222
BsaJI	CCNNGG	3 cut(s)	474, 919, 1281
BsaWI	WCCGGW	5 cut(s)	480, 557, 622, 1141, 1157
BsaXI	ACNNNNNCTCC	2 cut(s)	1188, 1218
Bsc4I	CCNNNNNNNGG	4 cut(s)	480, 952, 1070, 1415
Bse118I	RCCGGY	2 cut(s)	622, 1157
Bse1I	ACTGG	2 cut(s)	249, 860
Bse21I	CCTNAGG	2 cut(s)	576, 1026
Bse3DI	GCAATG	2 cut(s)	1003, 1134
BseAI	TCCGGA	1 cut(s)	1141
BseBI	CCWGG	1 cut(s)	476
BseDI	CCNNGG	3 cut(s)	474, 919, 1281
BseGI	GGATG	4 cut(s)	148, 378, 470, 658
BseLI	CCNNNNNNNGG	4 cut(s)	480, 952, 1070, 1415
BseMI	GCAATG	2 cut(s)	1003, 1134
BseMII	CTCAG	2 cut(s)	590, 1161
BseNI	ACTGG	2 cut(s)	249, 860
BseRI	GAGGAG	2 cut(s)	564, 1240
BseSI	GKGCMC	1 cut(s)	8
BseXI	GCAGC	6 cut(s)	72, 474, 519, 547, 550, 990
BsgI	GTGCAG	2 cut(s)	833, 932
Bsh1236I	CGCG	1 cut(s)	496
BshFI	GGCC	4 cut(s)	20, 941, 1044, 1408
BshNI	GGYRCC	2 cut(s)	58, 105
BshTI	ACCGGT	2 cut(s)	622, 1157
BsiHKAI	GWGCWC	2 cut(s)	1353, 1443
BsiHKCI	CYCGRG	1 cut(s)	1064
BsiSI	CCGG	5 cut(s)	481, 558, 623, 1142, 1158
BslFI	GGGAC	3 cut(s)	633, 752, 1305
BslI	CCNNNNNNNGG	4 cut(s)	480, 952, 1070, 1415
BsmAI	GTCTC	5 cut(s)	264, 425, 683, 1036, 1222
BsmBI	CGTCTC	1 cut(s)	425
BsmFI	GGGAC	3 cut(s)	633, 752, 1305
BsmI	GAATGC	3 cut(s)	762, 872, 900
BsnI	GGCC	4 cut(s)	20, 941, 1044, 1408
Bso31I	GGTCTC	2 cut(s)	1036, 1222
BsoBI	CYCGRG	1 cut(s)	1064
Bsp119I	TTCGAA	1 cut(s)	713
Bsp1286I	GDGCHC	3 cut(s)	8, 1353, 1443
Bsp13I	TCCGGA	1 cut(s)	1141
Bsp143I	GATC	6 cut(s)	145, 1009, 1021, 1212, 1237, 1387
Bsp1720I	GCTNAGC	1 cut(s)	1050
Bsp19I	CCATGG	1 cut(s)	1281
BspACI	CCGC	3 cut(s)	92, 525, 1289
BspANI	GGCC	4 cut(s)	20, 941, 1044, 1408
BspCNI	CTCAG	2 cut(s)	589, 1162
BspEI	TCCGGA	1 cut(s)	1141
BspFNI	CGCG	1 cut(s)	496
BspLI	GGNNCC	4 cut(s)	60, 107, 621, 1023
BspMAI	CTGCAG	1 cut(s)	537
BspMI	ACCTGC	1 cut(s)	954
BspPI	GGATC	4 cut(s)	140, 1016, 1029, 1382
BspQI	GCTCTTC	1 cut(s)	1380
BspT104I	TTCGAA	1 cut(s)	713
BspT107I	GGYRCC	2 cut(s)	58, 105
BspTNI	GGTCTC	2 cut(s)	1036, 1222
BsrDI	GCAATG	2 cut(s)	1003, 1134
BsrFI	RCCGGY	2 cut(s)	622, 1157
BsrI	ACTGG	2 cut(s)	249, 860
BssAI	RCCGGY	2 cut(s)	622, 1157
BssECI	CCNNGG	3 cut(s)	474, 919, 1281
BssMI	GATC	6 cut(s)	145, 1009, 1021, 1212, 1237, 1387
BssSI	CACGAG	2 cut(s)	782, 950
BssT1I	CCWWGG	1 cut(s)	1281
Bst2BI	CACGAG	2 cut(s)	782, 950
Bst2UI	CCWGG	1 cut(s)	476
Bst4CI	ACNGT	3 cut(s)	10, 346, 389
Bst6I	CTCTTC	1 cut(s)	1380
BstBI	TTCGAA	1 cut(s)	713
BstC8I	GCNNGC	1 cut(s)	1306
BstDEI	CTNAG	4 cut(s)	576, 1026, 1050, 1170
BstDSI	CCRYGG	1 cut(s)	1281
BstENI	CCTNNNNNAGG	1 cut(s)	950
BstF5I	GGATG	4 cut(s)	148, 378, 470, 658
BstFNI	CGCG	1 cut(s)	496
BstKTI	GATC	6 cut(s)	148, 1012, 1024, 1215, 1240, 1390
BstMAI	GTCTC	5 cut(s)	264, 425, 683, 1036, 1222
BstMBI	GATC	6 cut(s)	145, 1009, 1021, 1212, 1237, 1387
BstMWI	GCNNNNNNNGC	7 cut(s)	91, 280, 493, 538, 544, 856, 1333
BstNI	CCWGG	1 cut(s)	476
BstNSI	RCATGY	2 cut(s)	505, 1435
BstSCI	CCNGG	1 cut(s)	474
BstSFI	CTRYAG	2 cut(s)	533, 1233
BstSLI	GKGCMC	1 cut(s)	8
BstUI	CGCG	1 cut(s)	496
BstV1I	GCAGC	6 cut(s)	72, 474, 519, 547, 550, 990
BstV2I	GAAGAC	1 cut(s)	30
BstX2I	RGATCY	1 cut(s)	1021
BstYI	RGATCY	1 cut(s)	1021
Bsu36I	CCTNAGG	2 cut(s)	576, 1026
BsuRI	GGCC	4 cut(s)	20, 941, 1044, 1408
BtgI	CCRYGG	1 cut(s)	1281
BtsCI	GGATG	4 cut(s)	148, 378, 470, 658
BtsI	GCAGTG	1 cut(s)	795
BtsIMutI	CAGTG	5 cut(s)	6, 256, 795, 853, 1167
BveI	ACCTGC	1 cut(s)	954
Cac8I	GCNNGC	1 cut(s)	1306
Cfr10I	RCCGGY	2 cut(s)	622, 1157
Cfr13I	GGNCC	2 cut(s)	478, 620
Csp6I	GTAC	4 cut(s)	59, 106, 1199, 1361
CspAI	ACCGGT	2 cut(s)	622, 1157
CviAII	CATG	6 cut(s)	309, 502, 758, 824, 1282, 1432
CviQI	GTAC	4 cut(s)	59, 106, 1199, 1361
DdeI	CTNAG	4 cut(s)	576, 1026, 1050, 1170
DpnI	GATC	6 cut(s)	147, 1011, 1023, 1214, 1239, 1389
DpnII	GATC	6 cut(s)	145, 1009, 1021, 1212, 1237, 1387
EaeI	YGGCCR	1 cut(s)	18
Eam1104I	CTCTTC	1 cut(s)	1380
EarI	CTCTTC	1 cut(s)	1380
Ecl136II	GAGCTC	2 cut(s)	1351, 1441
Eco130I	CCWWGG	1 cut(s)	1281
Eco147I	AGGCCT	1 cut(s)	1408
Eco24I	GRGCYC	2 cut(s)	1353, 1443
Eco31I	GGTCTC	2 cut(s)	1036, 1222
Eco47I	GGWCC	2 cut(s)	478, 620
Eco53kI	GAGCTC	2 cut(s)	1351, 1441
Eco81I	CCTNAGG	2 cut(s)	576, 1026
Eco88I	CYCGRG	1 cut(s)	1064
EcoICRI	GAGCTC	2 cut(s)	1351, 1441
EcoNI	CCTNNNNNAGG	1 cut(s)	950
EcoRI	GAATTC	1 cut(s)	1163
EcoRII	CCWGG	1 cut(s)	474
EcoT14I	CCWWGG	1 cut(s)	1281
EcoT38I	GRGCYC	2 cut(s)	1353, 1443
ErhI	CCWWGG	1 cut(s)	1281
Esp3I	CGTCTC	1 cut(s)	425
FaeI	CATG	6 cut(s)	312, 505, 761, 827, 1285, 1435
FaqI	GGGAC	3 cut(s)	633, 752, 1305
FatI	CATG	6 cut(s)	308, 501, 757, 823, 1281, 1431
FauI	CCCGC	3 cut(s)	99, 518, 1282
FauNDI	CATATG	1 cut(s)	1258
FblI	GTMKAC	1 cut(s)	391
Fnu4HI	GCNGC	6 cut(s)	86, 488, 533, 536, 539, 1004
FokI	GGATG	4 cut(s)	155, 365, 457, 665
FriOI	GRGCYC	2 cut(s)	1353, 1443
Fsp4HI	GCNGC	6 cut(s)	86, 488, 533, 536, 539, 1004
FspBI	CTAG	5 cut(s)	582, 800, 878, 993, 1046
GluI	GCNGC	6 cut(s)	86, 488, 533, 536, 539, 1004
GsuI	CTGGAG	1 cut(s)	232
HaeIII	GGCC	4 cut(s)	20, 941, 1044, 1408
HapII	CCGG	5 cut(s)	481, 558, 623, 1142, 1158
Hin1II	CATG	6 cut(s)	312, 505, 761, 827, 1285, 1435
HincII	GTYRAC	3 cut(s)	392, 1247, 1315
HindII	GTYRAC	3 cut(s)	392, 1247, 1315
HinfI	GANTC	7 cut(s)	427, 591, 924, 1074, 1088, 1394, 1463
HpaII	CCGG	5 cut(s)	481, 558, 623, 1142, 1158
HphI	GGTGA	2 cut(s)	1004, 1172
Hpy166II	GTNNAC	3 cut(s)	392, 1247, 1315
Hpy188I	TCNGA	8 cut(s)	508, 596, 726, 920, 1036, 1180, 1344, 1399
Hpy188III	TCNNGA	9 cut(s)	265, 277, 424, 650, 863, 1064, 1142, 1391, 1467
Hpy8I	GTNNAC	3 cut(s)	392, 1247, 1315
Hpy99I	CGWCG	2 cut(s)	393, 396
HpyAV	CCTTC	1 cut(s)	1339
HpyCH4III	ACNGT	3 cut(s)	10, 346, 389
HpyCH4IV	ACGT	1 cut(s)	893
HpyCH4V	TGCA	6 cut(s)	72, 535, 698, 823, 850, 949
HpyF10VI	GCNNNNNNNGC	7 cut(s)	91, 280, 493, 538, 544, 856, 1333
HpyF3I	CTNAG	4 cut(s)	576, 1026, 1050, 1170
HpySE526I	ACGT	1 cut(s)	893
Hsp92II	CATG	6 cut(s)	312, 505, 761, 827, 1285, 1435
Kpn2I	TCCGGA	1 cut(s)	1141
KpnI	GGTACC	2 cut(s)	62, 109
Kzo9I	GATC	6 cut(s)	145, 1009, 1021, 1212, 1237, 1387
LguI	GCTCTTC	1 cut(s)	1380
LmnI	GCTCC	7 cut(s)	93, 197, 685, 897, 1144, 1348, 1446
Lsp1109I	GCAGC	6 cut(s)	72, 474, 519, 547, 550, 990
LweI	GCATC	2 cut(s)	578, 1443
MaeI	CTAG	5 cut(s)	582, 800, 878, 993, 1046
MaeII	ACGT	1 cut(s)	893
MaeIII	GTNAC	4 cut(s)	497, 806, 1130, 1445
MalI	GATC	6 cut(s)	147, 1011, 1023, 1214, 1239, 1389
MboI	GATC	6 cut(s)	145, 1009, 1021, 1212, 1237, 1387
MboII	GAAGA	4 cut(s)	35, 59, 319, 1367
MflI	RGATCY	1 cut(s)	1021
MhlI	GDGCHC	3 cut(s)	8, 1353, 1443
MluCI	AATT	6 cut(s)	122, 466, 747, 1163, 1299, 1487
MlyI	GAGTC	1 cut(s)	421
MmeI	TCCRAC	1 cut(s)	435
MroI	TCCGGA	1 cut(s)	1141
MroXI	GAANNNNTTC	1 cut(s)	1425
MseI	TTAA	1 cut(s)	1274
MslI	CAYNNNNRTG	3 cut(s)	546, 828, 1082
MspA1I	CMGCKG	2 cut(s)	94, 525
MspI	CCGG	5 cut(s)	481, 558, 623, 1142, 1158
MspR9I	CCNGG	1 cut(s)	476
Mva1269I	GAATGC	3 cut(s)	762, 872, 900
MvaI	CCWGG	1 cut(s)	476
MvnI	CGCG	1 cut(s)	496
MwoI	GCNNNNNNNGC	7 cut(s)	91, 280, 493, 538, 544, 856, 1333
NcoI	CCATGG	1 cut(s)	1281
NdeI	CATATG	1 cut(s)	1258
NdeII	GATC	6 cut(s)	145, 1009, 1021, 1212, 1237, 1387
NlaIII	CATG	6 cut(s)	312, 505, 761, 827, 1285, 1435
NlaIV	GGNNCC	4 cut(s)	60, 107, 621, 1023
NmuCI	GTSAC	2 cut(s)	806, 1445
NspI	RCATGY	2 cut(s)	505, 1435
NspV	TTCGAA	1 cut(s)	713
OliI	CACNNNNGTG	1 cut(s)	546
PceI	AGGCCT	1 cut(s)	1408
PciI	ACATGT	1 cut(s)	501
PciSI	GCTCTTC	1 cut(s)	1380
PctI	GAATGC	3 cut(s)	762, 872, 900
PdmI	GAANNNNTTC	1 cut(s)	1425
PfeI	GAWTC	6 cut(s)	591, 924, 1074, 1088, 1394, 1463
PinAI	ACCGGT	2 cut(s)	622, 1157
PkrI	GCNGC	6 cut(s)	87, 489, 534, 537, 540, 1005
PleI	GAGTC	1 cut(s)	421
PpsI	GAGTC	1 cut(s)	421
PscI	ACATGT	1 cut(s)	501
Psp124BI	GAGCTC	2 cut(s)	1353, 1443
Psp6I	CCWGG	1 cut(s)	474
PspGI	CCWGG	1 cut(s)	474
PspN4I	GGNNCC	4 cut(s)	60, 107, 621, 1023
PspPI	GGNCC	2 cut(s)	478, 620
PstI	CTGCAG	1 cut(s)	537
PsuI	RGATCY	1 cut(s)	1021
RsaI	GTAC	4 cut(s)	60, 107, 1200, 1362
RsaNI	GTAC	4 cut(s)	59, 106, 1199, 1361
RseI	CAYNNNNRTG	3 cut(s)	546, 828, 1082
SacI	GAGCTC	2 cut(s)	1353, 1443
SalI	GTCGAC	1 cut(s)	390
SapI	GCTCTTC	1 cut(s)	1380
SaqAI	TTAA	1 cut(s)	1274
SatI	GCNGC	6 cut(s)	86, 488, 533, 536, 539, 1004
Sau3AI	GATC	6 cut(s)	145, 1009, 1021, 1212, 1237, 1387
Sau96I	GGNCC	2 cut(s)	478, 620
ScaI	AGTACT	1 cut(s)	1200
SchI	GAGTC	1 cut(s)	421
ScrFI	CCNGG	1 cut(s)	476
SduI	GDGCHC	3 cut(s)	8, 1353, 1443
SfaNI	GCATC	2 cut(s)	578, 1443
SfcI	CTRYAG	2 cut(s)	533, 1233
SfuI	TTCGAA	1 cut(s)	713
SgrDI	CGTCGACG	1 cut(s)	390
SinI	GGWCC	2 cut(s)	478, 620
SmiMI	CAYNNNNRTG	3 cut(s)	546, 828, 1082
SmlI	CTYRAG	4 cut(s)	263, 680, 689, 1436
SmoI	CTYRAG	4 cut(s)	263, 680, 689, 1436
SpeI	ACTAGT	1 cut(s)	877
Sse9I	AATT	6 cut(s)	122, 466, 747, 1163, 1299, 1487
SseBI	AGGCCT	1 cut(s)	1408
SsiI	CCGC	3 cut(s)	92, 525, 1289
SspI	AATATT	1 cut(s)	117
SspMI	CTAG	5 cut(s)	582, 800, 878, 993, 1046
SstI	GAGCTC	2 cut(s)	1353, 1443
StuI	AGGCCT	1 cut(s)	1408
StyD4I	CCNGG	1 cut(s)	474
StyI	CCWWGG	1 cut(s)	1281
TaaI	ACNGT	3 cut(s)	10, 346, 389
TaiI	ACGT	1 cut(s)	896
TaqI	TCGA	5 cut(s)	391, 713, 771, 872, 1008
TasI	AATT	6 cut(s)	122, 466, 747, 1163, 1299, 1487
TatI	WGTACW	1 cut(s)	1198
TfiI	GAWTC	6 cut(s)	591, 924, 1074, 1088, 1394, 1463
Tru1I	TTAA	1 cut(s)	1274
Tru9I	TTAA	1 cut(s)	1274
TscAI	CASTG	5 cut(s)	13, 256, 795, 860, 1174
TseFI	GTSAC	2 cut(s)	806, 1445
TseI	GCWGC	6 cut(s)	85, 487, 532, 535, 538, 1003
Tsp45I	GTSAC	2 cut(s)	806, 1445
TspDTI	ATGAA	3 cut(s)	319, 459, 533
TspGWI	ACGGA	2 cut(s)	407, 1433
TspRI	CASTG	5 cut(s)	13, 256, 795, 860, 1174
VpaK11BI	GGWCC	2 cut(s)	478, 620
XagI	CCTNNNNNAGG	1 cut(s)	950
XapI	RAATTY	2 cut(s)	122, 1163
XceI	RCATGY	2 cut(s)	505, 1435
XmiI	GTMKAC	1 cut(s)	391
XmnI	GAANNNNTTC	1 cut(s)	1425
XspI	CTAG	5 cut(s)	582, 800, 878, 993, 1046
ZrmI	AGTACT	1 cut(s)	1200

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.