MD01G1228100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012071
Physical Location & Seq
Forward (+)
31798763 .. 31801145
2383 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1228100.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGCTCTGTTCCGTGCCAGCGACCAAGTCCGGGTCGAACTGGCTGAACCGGCTCCGGTCCAACAAGGGCTTCCCCACCTGCGAGAGTTCCGACCTCGACCACTTCCTTACCCGAAACCTAAGCTCTTCCTCGGAATTCCCGAATCCCAATGTTGTCTCACCATCCACAGATTCGACTCGCCCGGATTCGGACCGAGTCGATAATCGCTACGAAACATCGTTCCCCAACCGAGACGACCAGAGAGGGGCAATCATCGGGATGATGAGCAATGTTCTGTCGGAGCTATTTTTCATGGGCGGCGCCGACGAAAGCTCTAAAATTTCTGGGAAAAAGATTCCTAGAAAGCAAGCAAACCCCAGAGTCTGTGTCGCTTCCTCGACCAACTCCAATGGCGACGCCACTGCAAATGCAGCGGAAGAGAAGAGCTCCGGCTGCGAGCGGAATGTCGAAAATGCCGTCGACAAGTCGGCGTCCGATAACAGCAGCTTGGTGGGGAAGAAGAAGAAGAAGAAGAAGAAGAATGAAGTAGGAGGGAATGTGGGTGGTGGGGAATGTGAGGGGGAGGAGGAGGAGGAGGAGGAAGATAAGGGGGAGATGAAAGGGTATTCGAGAAGCGAAGTGACGGTGATCGATACGAGCTGCGGGGTTTGGAAGACGGATAAGTTGGTGTTTAGGAGGAAGAATGTGTGGAAAGTTAGGGAGAAGAAGGTCAAGGGGAGGAGCTCAGGGAGACATAAGAGGAAGGTGGTTGATGAGGAACATGTTGGTGATGACATTGACAAAAAGAAAGCTAAGGTTTCAGTTTCGGCTTTGGATGTTGAAGCTGGTGGGGATGAATGTATAGCTCTCAACTCCATTCATGAAGGACAGAAGCCGCAAAATGACATGCTAGATAATCTTAATGACAGAGGGGAAGAATTCGGCAAATACCTGCCGGATAATAAAACCAAAGATAGAAGAAAAGGGTTTCCTTTCAACCGAACAATGAGAACTTTGGAAAAGCGTGGCTCCTCTGTTATATGTATTAAAGGCATTCCTACAGATAGTAAAAAAGGAGGAAAGCTTCATAGGAAGTGTCTCAAGGACAGCCAAAAACAATACAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

370

Amino Acids

40.79

Weight (kDa)

9.13

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24500
fragaria_vesca FvH4_7g32890 FvH4_7g32890
malus_domestica MD01G1228100.v1.1 MD07G1298800.v1.1
prunus_persica Prupe.2G318600_v2.0.a1
pyrus_communis pycom01g23630 pycom07g27120
rosa_chinensis RchiOBHm_Chr1g0382841
rosa_laevigata RLG00000026155
rosa_multiflora Rmu_co8155456.1_g000001 Rmu_sc0003389.1_g000012
rosa_roxburghii Rroxscaffold_4G00277850
rosa_rugosa Rorug01G0436700
rosa_samantha Rh1AG461700 Rh1BG418700 Rh1CG432300 Rh1DG450800
rosa_wichuraiana Rw1G041010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 86
Acc36I ACCTGC 2 cut(s) 86, 939
AccB1I GGYRCC 1 cut(s) 299
AccBSI CCGCTC 1 cut(s) 439
AccI GTMKAC 1 cut(s) 459
AciI CCGC 5 cut(s) 297, 413, 439, 642, 875
AcsI RAATTY 3 cut(s) 134, 318, 917
AcyI GRCGYC 3 cut(s) 300, 396, 470
AfiI CCNNNNNNNGG 3 cut(s) 30, 187, 244
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 2 cut(s) 821, 976
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
Alw21I GWGCWC 2 cut(s) 428, 725
Alw26I GTCTC 4 cut(s) 160, 225, 724, 1082
AlwNI CAGNNNCTG 1 cut(s) 363
ApeKI GCWGC 4 cut(s) 410, 432, 483, 639
ApoI RAATTY 3 cut(s) 134, 318, 917
ArsI GACNNNNNNTTYG 2 cut(s) 441, 473
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 2 cut(s) 57, 190
AsuC2I CCSGG 2 cut(s) 31, 182
AsuHPI GGTGA 3 cut(s) 150, 637, 779
AvaII GGWCC 2 cut(s) 57, 190
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 2 cut(s) 428, 725
BbsI GAAGAC 1 cut(s) 659
Bbv12I GWGCWC 2 cut(s) 428, 725
BbvI GCAGC 4 cut(s) 419, 422, 495, 626
BccI CCATC 1 cut(s) 169
BceAI ACGGC 1 cut(s) 440
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BcnI CCSGG 2 cut(s) 31, 182
BcoDI GTCTC 4 cut(s) 160, 225, 724, 1082
BfaI CTAG 2 cut(s) 339, 890
BfmI CTRYAG 1 cut(s) 1038
BfoI RGCGCY 1 cut(s) 303
BfuAI ACCTGC 2 cut(s) 86, 939
BisI GCNGC 6 cut(s) 298, 411, 433, 484, 640, 875
BlsI GCNGC 6 cut(s) 299, 412, 434, 485, 641, 876
Bme1390I CCNGG 2 cut(s) 31, 182
Bme18I GGWCC 2 cut(s) 57, 190
BmgT120I GGNCC 2 cut(s) 57, 190
BmiI GGNNCC 3 cut(s) 53, 301, 1009
BmrFI CCNGG 2 cut(s) 31, 182
BpiI GAAGAC 1 cut(s) 659
BplI GAGNNNNNCTC 2 cut(s) 410, 442
Bpu10I CCTNAGC 3 cut(s) 119, 724, 792
BpuEI CTTGAG 1 cut(s) 1064
BpuMI CCSGG 2 cut(s) 31, 182
Bsa29I ATCGAT 1 cut(s) 630
BsaHI GRCGYC 3 cut(s) 300, 396, 470
BsaJI CCNNGG 1 cut(s) 129
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 187, 244
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 1 cut(s) 44
Bse3DI GCAATG 1 cut(s) 274
BseCI ATCGAT 1 cut(s) 630
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 4 cut(s) 161, 264, 820, 838
BseLI CCNNNNNNNGG 3 cut(s) 30, 187, 244
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 1 cut(s) 44
BseRI GAGGAG 7 cut(s) 578, 581, 584, 587, 590, 733, 1000
BseXI GCAGC 4 cut(s) 419, 422, 495, 626
BshNI GGYRCC 1 cut(s) 299
BshVI ATCGAT 1 cut(s) 630
BsiHKAI GWGCWC 2 cut(s) 428, 725
BsiSI CCGG 6 cut(s) 30, 49, 55, 182, 429, 935
BslI CCNNNNNNNGG 3 cut(s) 30, 187, 244
BsmAI GTCTC 4 cut(s) 160, 225, 724, 1082
BsmBI CGTCTC 1 cut(s) 225
BsmI GAATGC 1 cut(s) 1032
Bsp1286I GDGCHC 2 cut(s) 428, 725
Bsp143I GATC 1 cut(s) 627
BspACI CCGC 5 cut(s) 297, 413, 439, 642, 875
BspCNI CTCAG 1 cut(s) 737
BspDI ATCGAT 1 cut(s) 630
BspHI TCATGA 1 cut(s) 859
BspLI GGNNCC 3 cut(s) 53, 301, 1009
BspMI ACCTGC 2 cut(s) 86, 939
BspQI GCTCTTC 2 cut(s) 130, 416
BspT107I GGYRCC 1 cut(s) 299
BsrBI CCGCTC 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 274
BsrFI RCCGGY 1 cut(s) 48
BsrI ACTGG 1 cut(s) 44
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 1 cut(s) 627
BssNI GRCGYC 3 cut(s) 300, 396, 470
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 3 cut(s) 130, 411, 416
BstACI GRCGYC 3 cut(s) 300, 396, 470
BstC8I GCNNGC 3 cut(s) 18, 348, 437
BstDEI CTNAG 3 cut(s) 119, 724, 792
BstF5I GGATG 4 cut(s) 161, 264, 820, 838
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 1 cut(s) 630
BstMAI GTCTC 4 cut(s) 160, 225, 724, 1082
BstMBI GATC 1 cut(s) 627
BstMWI GCNNNNNNNGC 3 cut(s) 49, 410, 432
BstNSI RCATGY 2 cut(s) 764, 889
BstSCI CCNGG 2 cut(s) 29, 180
BstSFI CTRYAG 1 cut(s) 1038
BstV1I GCAGC 4 cut(s) 419, 422, 495, 626
BstV2I GAAGAC 1 cut(s) 659
Bsu15I ATCGAT 1 cut(s) 630
BsuTUI ATCGAT 1 cut(s) 630
BtsCI GGATG 4 cut(s) 161, 264, 820, 838
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 1 cut(s) 399
BveI ACCTGC 2 cut(s) 86, 939
Cac8I GCNNGC 3 cut(s) 18, 348, 437
CaiI CAGNNNCTG 1 cut(s) 363
CciI TCATGA 1 cut(s) 859
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 57, 190
ClaI ATCGAT 1 cut(s) 630
CpoI CGGWCCG 1 cut(s) 190
CseI GACGC 2 cut(s) 404, 459
CspI CGGWCCG 1 cut(s) 190
CviAII CATG 4 cut(s) 292, 761, 860, 886
DdeI CTNAG 3 cut(s) 119, 724, 792
DinI GGCGCC 1 cut(s) 301
DpnI GATC 1 cut(s) 629
DpnII GATC 1 cut(s) 627
Eam1104I CTCTTC 3 cut(s) 130, 411, 416
EarI CTCTTC 3 cut(s) 130, 411, 416
Ecl136II GAGCTC 2 cut(s) 426, 723
Eco24I GRGCYC 2 cut(s) 428, 725
Eco47I GGWCC 2 cut(s) 57, 190
Eco53kI GAGCTC 2 cut(s) 426, 723
EcoICRI GAGCTC 2 cut(s) 426, 723
EcoRI GAATTC 2 cut(s) 134, 917
EcoT38I GRGCYC 2 cut(s) 428, 725
EgeI GGCGCC 1 cut(s) 301
EheI GGCGCC 1 cut(s) 301
Esp3I CGTCTC 1 cut(s) 225
FaeI CATG 4 cut(s) 295, 764, 863, 889
FaiI YATR 9 cut(s) 293, 735, 762, 842, 861, 887, 1019, 1021, 1068
FatI CATG 4 cut(s) 291, 760, 859, 885
FauI CCCGC 1 cut(s) 635
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 6 cut(s) 298, 411, 433, 484, 640, 875
FokI GGATG 4 cut(s) 148, 271, 827, 845
FriOI GRGCYC 2 cut(s) 428, 725
Fsp4HI GCNGC 6 cut(s) 298, 411, 433, 484, 640, 875
FspBI CTAG 2 cut(s) 339, 890
GlaI GCGC 1 cut(s) 301
GluI GCNGC 6 cut(s) 298, 411, 433, 484, 640, 875
HaeII RGCGCY 1 cut(s) 303
HapII CCGG 6 cut(s) 30, 49, 55, 182, 429, 935
HgaI GACGC 2 cut(s) 404, 459
HhaI GCGC 1 cut(s) 302
Hin1I GRCGYC 3 cut(s) 300, 396, 470
Hin1II CATG 4 cut(s) 295, 764, 863, 889
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 2 cut(s) 1061, 1104
HinfI GANTC 7 cut(s) 142, 170, 175, 185, 195, 334, 360
HpaII CCGG 6 cut(s) 30, 49, 55, 182, 429, 935
HphI GGTGA 3 cut(s) 150, 637, 779
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 5 cut(s) 91, 133, 190, 280, 475
Hpy188III TCNNGA 4 cut(s) 139, 256, 609, 860
Hpy8I GTNNAC 1 cut(s) 460
Hpy99I CGWCG 3 cut(s) 308, 398, 461
HpyAV CCTTC 3 cut(s) 700, 736, 857
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4V TGCA 2 cut(s) 404, 410
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 410, 432
HpyF3I CTNAG 3 cut(s) 119, 724, 792
Hsp92I GRCGYC 3 cut(s) 300, 396, 470
Hsp92II CATG 4 cut(s) 295, 764, 863, 889
HspAI GCGC 1 cut(s) 300
KasI GGCGCC 1 cut(s) 299
Kzo9I GATC 1 cut(s) 627
LguI GCTCTTC 2 cut(s) 130, 416
LmnI GCTCC 5 cut(s) 57, 280, 431, 720, 1013
Lsp1109I GCAGC 4 cut(s) 419, 422, 495, 626
MaeI CTAG 2 cut(s) 339, 890
MaeIII GTNAC 1 cut(s) 619
MalI GATC 1 cut(s) 629
MbiI CCGCTC 1 cut(s) 439
MboI GATC 1 cut(s) 627
MhlI GDGCHC 2 cut(s) 428, 725
MluCI AATT 3 cut(s) 134, 318, 917
Mly113I GGCGCC 1 cut(s) 300
MlyI GAGTC 3 cut(s) 169, 204, 369
MmeI TCCRAC 3 cut(s) 84, 114, 258
MseI TTAA 2 cut(s) 900, 1026
MslI CAYNNNNRTG 2 cut(s) 257, 765
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 6 cut(s) 30, 49, 55, 182, 429, 935
MspR9I CCNGG 2 cut(s) 31, 182
Mva1269I GAATGC 1 cut(s) 1032
MwoI GCNNNNNNNGC 3 cut(s) 49, 410, 432
NarI GGCGCC 1 cut(s) 300
NciI CCSGG 2 cut(s) 31, 182
NdeII GATC 1 cut(s) 627
NlaIII CATG 4 cut(s) 295, 764, 863, 889
NlaIV GGNNCC 3 cut(s) 53, 301, 1009
NmuCI GTSAC 1 cut(s) 619
NspI RCATGY 2 cut(s) 764, 889
PagI TCATGA 1 cut(s) 859
PaqCI CACCTGC 1 cut(s) 86
PciI ACATGT 1 cut(s) 760
PciSI GCTCTTC 2 cut(s) 130, 416
PcsI WCGNNNNNNNCGW 2 cut(s) 137, 453
PctI GAATGC 1 cut(s) 1032
PfeI GAWTC 4 cut(s) 142, 170, 185, 334
PflFI GACNNNGTC 2 cut(s) 25, 194
PkrI GCNGC 6 cut(s) 299, 412, 434, 485, 641, 876
PleI GAGTC 3 cut(s) 169, 203, 368
PluTI GGCGCC 1 cut(s) 303
PpsI GAGTC 3 cut(s) 169, 203, 368
PscI ACATGT 1 cut(s) 760
Psp124BI GAGCTC 2 cut(s) 428, 725
PspN4I GGNNCC 3 cut(s) 53, 301, 1009
PspPI GGNCC 2 cut(s) 57, 190
PstNI CAGNNNCTG 1 cut(s) 363
PsyI GACNNNGTC 2 cut(s) 25, 194
RseI CAYNNNNRTG 2 cut(s) 257, 765
Rsr2I CGGWCCG 1 cut(s) 190
RsrII CGGWCCG 1 cut(s) 190
SacI GAGCTC 2 cut(s) 428, 725
SalI GTCGAC 1 cut(s) 458
SapI GCTCTTC 2 cut(s) 130, 416
SaqAI TTAA 2 cut(s) 900, 1026
SatI GCNGC 6 cut(s) 298, 411, 433, 484, 640, 875
Sau3AI GATC 1 cut(s) 627
Sau96I GGNCC 2 cut(s) 57, 190
SchI GAGTC 3 cut(s) 169, 204, 369
ScrFI CCNGG 2 cut(s) 31, 182
SduI GDGCHC 2 cut(s) 428, 725
SfcI CTRYAG 1 cut(s) 1038
SfoI GGCGCC 1 cut(s) 301
SinI GGWCC 2 cut(s) 57, 190
SmiMI CAYNNNNRTG 2 cut(s) 257, 765
SmlI CTYRAG 1 cut(s) 1079
SmoI CTYRAG 1 cut(s) 1079
Sse9I AATT 3 cut(s) 134, 318, 917
SsiI CCGC 5 cut(s) 297, 413, 439, 642, 875
SspDI GGCGCC 1 cut(s) 299
SspMI CTAG 2 cut(s) 339, 890
SstI GAGCTC 2 cut(s) 428, 725
StyD4I CCNGG 2 cut(s) 29, 180
TaaI ACNGT 1 cut(s) 625
TaqI TCGA 9 cut(s) 35, 96, 173, 198, 377, 447, 459, 608, 630
TaqII GACCGA 1 cut(s) 207
TasI AATT 3 cut(s) 134, 318, 917
TauI GCSGC 2 cut(s) 300, 877
TfiI GAWTC 4 cut(s) 142, 170, 185, 334
Tru1I TTAA 2 cut(s) 900, 1026
Tru9I TTAA 2 cut(s) 900, 1026
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 4 cut(s) 410, 432, 483, 639
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 7 cut(s) 280, 537, 611, 848, 849, 876, 1055
TspGWI ACGGA 1 cut(s) 671
TspRI CASTG 1 cut(s) 406
Tth111I GACNNNGTC 2 cut(s) 25, 194
VpaK11BI GGWCC 2 cut(s) 57, 190
XapI RAATTY 3 cut(s) 134, 318, 917
XceI RCATGY 2 cut(s) 764, 889
XmiI GTMKAC 1 cut(s) 459
XspI CTAG 2 cut(s) 339, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.