MD07G1298800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012071
Physical Location & Seq
Forward (+)
35820946 .. 35826495
5550 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1298800.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGCTCTGTTCGGTGCCGGCCACCAAGTCCGGTTCGAACTGGCTGAACCGGCTCCGGTCCAACAAGGGATTCCCGACCGCCGACAATTCCGACCTCGACCACTTCCTTACCCGAAACGCAAGCTCTCTCTGTGAATTCCCGGCTCCCAATGTCGTCTCATCGTCCACGGATTCGACTCGGCCGGATTCGGACCGTGTCGGGAATCGCTCCGAATCCTTGTGTCCGAATGGCGATGACCAGGGAGGGGAAATCATCGGAATGATGAGCAACGTTCTGTCGGAGCTATTTTTCATGGGCGGCGCCGACGAAAGTTCCAGACTTTCTGGGAAAAAGATTCCTAGAAAGCAAGCAAACCCCAGAGTCTGTGTCGGTTCCACGACCAACTCTAACGGCGACGCCACCGCCACTGCGAATGAAGCGGAGGAGAAGAGCTCGGAAAGTGAAGGAAATATCGAAAATGCCCTCGACAAGGTGGCGTCGGATAACAACAGCTTGGTGGGGAAGAAGAAGAAGAAGGAACTAGGAGGGAATGTGGGTGGTGGGCAATGTGAGGGGGAGGAGGATGAGGAGGAGGAAGAGAAGGGGGAGATGAAAGGGTATTCGAGGAGCGAAGTGACGGTGATTGATACGAGCTGCGGGGTTTGGAAGACGGAGAAGTTGGTGTTTAGGAGGAAGAATGTGTGGAAAGTTAGGGAGAAGAAGGTCAAAGGGAGGAGCTTTGGGAGACATAAGAGGAAAGTGGTTGTTGAGGAACACGTTGCTGATGACATTGACAAGAAGAAAGCTAAGGTTTCGGTTTCCGCGTTGGATGTTGAAGCTGGTGGGGATGAATGTATAGCTCTCAACTCCATTCAAGTACAGAAGCCACAGAATGACACCCTAGATAATCTGAATGACAGAGGGGAAGAATTCGGCAAACACCTGCCGGAAAATAAAACCAAAGATACAAGAAAAGGGTTTTCTTTGAACCGATCACCGCGAAATTTGAAAAAGGGCGGTTCCTCTGTTATATTAATAAAAGGCCTTCCTACAGACAATAAAAATGGAGGAAAGCTTCATAAAAAGACTCTCAAGGACAGCCAAAAACAATGCAAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

367

Amino Acids

39.84

Weight (kDa)

8.73

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24500
fragaria_vesca FvH4_7g32890 FvH4_7g32890
malus_domestica MD01G1228100.v1.1 MD07G1298800.v1.1
prunus_persica Prupe.2G318600_v2.0.a1
pyrus_communis pycom01g23630 pycom07g27120
rosa_chinensis RchiOBHm_Chr1g0382841
rosa_laevigata RLG00000026155
rosa_multiflora Rmu_co8155456.1_g000001 Rmu_sc0003389.1_g000012
rosa_roxburghii Rroxscaffold_4G00277850
rosa_rugosa Rorug01G0436700
rosa_samantha Rh1AG461700 Rh1BG418700 Rh1CG432300 Rh1DG450800
rosa_wichuraiana Rw1G041010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 930
Acc36I ACCTGC 1 cut(s) 930
AccB1I GGYRCC 2 cut(s) 13, 299
AccII CGCG 2 cut(s) 803, 979
AciI CCGC 8 cut(s) 78, 297, 402, 419, 636, 801, 977, 996
AclI AACGTT 1 cut(s) 270
AcoI YGGCCR 2 cut(s) 18, 179
AcsI RAATTY 3 cut(s) 134, 908, 982
AcyI GRCGYC 3 cut(s) 300, 396, 476
AfaI GTAC 1 cut(s) 858
AfiI CCNNNNNNNGG 2 cut(s) 244, 469
AflIII ACRYGT 1 cut(s) 754
AgsI TTSAA 4 cut(s) 815, 854, 967, 988
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
Alw21I GWGCWC 1 cut(s) 434
Alw26I GTCTC 2 cut(s) 160, 718
AlwNI CAGNNNCTG 1 cut(s) 363
AoxI GGCC 3 cut(s) 18, 179, 1021
ApeKI GCWGC 1 cut(s) 633
ApoI RAATTY 3 cut(s) 134, 908, 982
AseI ATTAAT 1 cut(s) 1013
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 2 cut(s) 57, 190
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 631, 966
AsuII TTCGAA 1 cut(s) 35
AvaII GGWCC 2 cut(s) 57, 190
BanI GGYRCC 2 cut(s) 13, 299
BanII GRGCYC 1 cut(s) 434
BbsI GAAGAC 1 cut(s) 653
Bbv12I GWGCWC 1 cut(s) 434
BbvI GCAGC 1 cut(s) 620
BceAI ACGGC 1 cut(s) 406
BciT130I CCWGG 1 cut(s) 239
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 160, 718
BfaI CTAG 3 cut(s) 339, 521, 881
BfmI CTRYAG 1 cut(s) 1029
BfoI RGCGCY 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 930
BisI GCNGC 2 cut(s) 298, 634
BlsI GCNGC 2 cut(s) 299, 635
Bme1390I CCNGG 2 cut(s) 140, 239
Bme18I GGWCC 2 cut(s) 57, 190
BmgT120I GGNCC 2 cut(s) 57, 190
BmiI GGNNCC 6 cut(s) 15, 53, 144, 301, 373, 1000
BmrFI CCNGG 2 cut(s) 140, 239
BpiI GAAGAC 1 cut(s) 653
BplI GAGNNNNNCTC 2 cut(s) 416, 448
Bpu10I CCTNAGC 1 cut(s) 786
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 1055
BpuMI CCSGG 1 cut(s) 140
BsaHI GRCGYC 3 cut(s) 300, 396, 476
BsaJI CCNNGG 2 cut(s) 165, 238
BsaWI WCCGGW 2 cut(s) 29, 54
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 469
Bse118I RCCGGY 2 cut(s) 16, 48
Bse1I ACTGG 1 cut(s) 44
Bse3DI GCAATG 1 cut(s) 551
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 165, 238
BseGI GGATG 3 cut(s) 568, 814, 832
BseLI CCNNNNNNNGG 2 cut(s) 244, 469
BseMI GCAATG 1 cut(s) 551
BseNI ACTGG 1 cut(s) 44
BseRI GAGGAG 6 cut(s) 437, 572, 581, 584, 619, 727
BseX3I CGGCCG 1 cut(s) 179
BseXI GCAGC 1 cut(s) 620
Bsh1236I CGCG 2 cut(s) 803, 979
Bsh1285I CGRYCG 2 cut(s) 78, 182
BshFI GGCC 3 cut(s) 20, 181, 1023
BshNI GGYRCC 2 cut(s) 13, 299
BsiEI CGRYCG 2 cut(s) 78, 182
BsiHKAI GWGCWC 1 cut(s) 434
BsiSI CCGG 7 cut(s) 17, 30, 49, 55, 140, 182, 926
BslI CCNNNNNNNGG 2 cut(s) 244, 469
BsmAI GTCTC 2 cut(s) 160, 718
BsmBI CGTCTC 1 cut(s) 160
BsnI GGCC 3 cut(s) 20, 181, 1023
Bsp119I TTCGAA 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 434
Bsp143I GATC 1 cut(s) 971
BspACI CCGC 8 cut(s) 78, 297, 402, 419, 636, 801, 977, 996
BspANI GGCC 3 cut(s) 20, 181, 1023
BspFNI CGCG 2 cut(s) 803, 979
BspLI GGNNCC 6 cut(s) 15, 53, 144, 301, 373, 1000
BspMI ACCTGC 1 cut(s) 930
BspQI GCTCTTC 1 cut(s) 422
BspT104I TTCGAA 1 cut(s) 35
BspT107I GGYRCC 2 cut(s) 13, 299
BsrDI GCAATG 1 cut(s) 551
BsrFI RCCGGY 2 cut(s) 16, 48
BsrI ACTGG 1 cut(s) 44
BssAI RCCGGY 2 cut(s) 16, 48
BssECI CCNNGG 2 cut(s) 165, 238
BssMI GATC 1 cut(s) 971
BssNI GRCGYC 3 cut(s) 300, 396, 476
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 2 cut(s) 194, 619
Bst6I CTCTTC 2 cut(s) 422, 570
BstACI GRCGYC 3 cut(s) 300, 396, 476
BstBI TTCGAA 1 cut(s) 35
BstC8I GCNNGC 3 cut(s) 18, 121, 348
BstDEI CTNAG 1 cut(s) 786
BstDSI CCRYGG 1 cut(s) 165
BstENI CCTNNNNNAGG 1 cut(s) 467
BstF5I GGATG 3 cut(s) 568, 814, 832
BstFNI CGCG 2 cut(s) 803, 979
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 1 cut(s) 974
BstMAI GTCTC 2 cut(s) 160, 718
BstMBI GATC 1 cut(s) 971
BstMCI CGRYCG 2 cut(s) 78, 182
BstMWI GCNNNNNNNGC 2 cut(s) 49, 416
BstNI CCWGG 1 cut(s) 239
BstSCI CCNGG 2 cut(s) 138, 237
BstSFI CTRYAG 1 cut(s) 1029
BstUI CGCG 2 cut(s) 803, 979
BstV1I GCAGC 1 cut(s) 620
BstV2I GAAGAC 1 cut(s) 653
BstZI CGGCCG 1 cut(s) 179
BsuRI GGCC 3 cut(s) 20, 181, 1023
BtgI CCRYGG 1 cut(s) 165
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 3 cut(s) 568, 814, 832
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 405
BveI ACCTGC 1 cut(s) 930
Cac8I GCNNGC 3 cut(s) 18, 121, 348
CaiI CAGNNNCTG 1 cut(s) 363
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 2 cut(s) 16, 48
Cfr13I GGNCC 2 cut(s) 57, 190
CpoI CGGWCCG 1 cut(s) 190
CseI GACGC 2 cut(s) 404, 465
Csp6I GTAC 1 cut(s) 857
CspI CGGWCCG 1 cut(s) 190
CviAII CATG 1 cut(s) 292
CviQI GTAC 1 cut(s) 857
DdeI CTNAG 1 cut(s) 786
DinI GGCGCC 1 cut(s) 301
DpnI GATC 1 cut(s) 973
DpnII GATC 1 cut(s) 971
EaeI YGGCCR 2 cut(s) 18, 179
EagI CGGCCG 1 cut(s) 179
Eam1104I CTCTTC 2 cut(s) 422, 570
EarI CTCTTC 2 cut(s) 422, 570
Ecl136II GAGCTC 1 cut(s) 432
EclXI CGGCCG 1 cut(s) 179
Eco147I AGGCCT 1 cut(s) 1023
Eco24I GRGCYC 1 cut(s) 434
Eco47I GGWCC 2 cut(s) 57, 190
Eco52I CGGCCG 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 432
EcoICRI GAGCTC 1 cut(s) 432
EcoNI CCTNNNNNAGG 1 cut(s) 467
EcoRI GAATTC 2 cut(s) 134, 908
EcoRII CCWGG 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 434
EgeI GGCGCC 1 cut(s) 301
EheI GGCGCC 1 cut(s) 301
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 1 cut(s) 295
FaiI YATR 5 cut(s) 293, 729, 836, 1010, 1059
FatI CATG 1 cut(s) 291
FauI CCCGC 1 cut(s) 629
Fnu4HI GCNGC 2 cut(s) 298, 634
FokI GGATG 3 cut(s) 575, 821, 839
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 2 cut(s) 298, 634
FspBI CTAG 3 cut(s) 339, 521, 881
GlaI GCGC 1 cut(s) 301
GluI GCNGC 2 cut(s) 298, 634
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 3 cut(s) 20, 181, 1023
HapII CCGG 7 cut(s) 17, 30, 49, 55, 140, 182, 926
HgaI GACGC 2 cut(s) 404, 465
HhaI GCGC 1 cut(s) 302
Hin1I GRCGYC 3 cut(s) 300, 396, 476
Hin1II CATG 1 cut(s) 295
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 1052
HinfI GANTC 9 cut(s) 69, 170, 175, 185, 202, 212, 334, 360, 1066
HpaII CCGG 7 cut(s) 17, 30, 49, 55, 140, 182, 926
HphI GGTGA 2 cut(s) 631, 966
Hpy166II GTNNAC 1 cut(s) 165
Hpy188I TCNGA 9 cut(s) 91, 190, 211, 225, 257, 280, 436, 481, 891
Hpy188III TCNNGA 3 cut(s) 73, 199, 315
Hpy8I GTNNAC 1 cut(s) 165
Hpy99I CGWCG 3 cut(s) 308, 398, 481
HpyAV CCTTC 5 cut(s) 437, 508, 574, 694, 1034
HpyCH4III ACNGT 2 cut(s) 194, 619
HpyCH4IV ACGT 2 cut(s) 270, 756
HpyCH4V TGCA 1 cut(s) 1092
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 416
HpyF3I CTNAG 1 cut(s) 786
HpySE526I ACGT 2 cut(s) 270, 756
Hsp92I GRCGYC 3 cut(s) 300, 396, 476
Hsp92II CATG 1 cut(s) 295
HspAI GCGC 1 cut(s) 300
KasI GGCGCC 1 cut(s) 299
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
Kzo9I GATC 1 cut(s) 971
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 6 cut(s) 57, 148, 212, 280, 606, 714
Lsp1109I GCAGC 1 cut(s) 620
MaeI CTAG 3 cut(s) 339, 521, 881
MaeII ACGT 2 cut(s) 270, 756
MaeIII GTNAC 1 cut(s) 613
MalI GATC 1 cut(s) 973
MboI GATC 1 cut(s) 971
MhlI GDGCHC 1 cut(s) 434
MluCI AATT 4 cut(s) 85, 134, 908, 982
Mly113I GGCGCC 1 cut(s) 300
MlyI GAGTC 3 cut(s) 169, 369, 1060
MmeI TCCRAC 5 cut(s) 84, 114, 258, 459, 786
MroNI GCCGGC 1 cut(s) 16
MseI TTAA 1 cut(s) 1013
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 7 cut(s) 17, 30, 49, 55, 140, 182, 926
MspR9I CCNGG 2 cut(s) 140, 239
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 2 cut(s) 803, 979
MwoI GCNNNNNNNGC 2 cut(s) 49, 416
NaeI GCCGGC 1 cut(s) 18
NarI GGCGCC 1 cut(s) 300
NciI CCSGG 1 cut(s) 140
NdeII GATC 1 cut(s) 971
NgoMIV GCCGGC 1 cut(s) 16
NlaIII CATG 1 cut(s) 295
NlaIV GGNNCC 6 cut(s) 15, 53, 144, 301, 373, 1000
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 613
NspV TTCGAA 1 cut(s) 35
PaqCI CACCTGC 1 cut(s) 930
PceI AGGCCT 1 cut(s) 1023
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 800
PdiI GCCGGC 1 cut(s) 18
PfeI GAWTC 6 cut(s) 69, 170, 185, 202, 212, 334
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 2 cut(s) 299, 635
PleI GAGTC 3 cut(s) 169, 368, 1060
PluTI GGCGCC 1 cut(s) 303
PpsI GAGTC 3 cut(s) 169, 368, 1060
PshBI ATTAAT 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 434
Psp1406I AACGTT 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 6 cut(s) 15, 53, 144, 301, 373, 1000
PspPI GGNCC 2 cut(s) 57, 190
PstNI CAGNNNCTG 1 cut(s) 363
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 1 cut(s) 858
RsaNI GTAC 1 cut(s) 857
RseI CAYNNNNRTG 1 cut(s) 257
Rsr2I CGGWCCG 1 cut(s) 190
RsrII CGGWCCG 1 cut(s) 190
SacI GAGCTC 1 cut(s) 434
SapI GCTCTTC 1 cut(s) 422
SaqAI TTAA 1 cut(s) 1013
SatI GCNGC 2 cut(s) 298, 634
Sau3AI GATC 1 cut(s) 971
Sau96I GGNCC 2 cut(s) 57, 190
SchI GAGTC 3 cut(s) 169, 369, 1060
ScrFI CCNGG 2 cut(s) 140, 239
SduI GDGCHC 1 cut(s) 434
SfcI CTRYAG 1 cut(s) 1029
SfoI GGCGCC 1 cut(s) 301
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 2 cut(s) 57, 190
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 1070
SmoI CTYRAG 1 cut(s) 1070
Sse9I AATT 4 cut(s) 85, 134, 908, 982
SseBI AGGCCT 1 cut(s) 1023
SsiI CCGC 8 cut(s) 78, 297, 402, 419, 636, 801, 977, 996
SspDI GGCGCC 1 cut(s) 299
SspMI CTAG 3 cut(s) 339, 521, 881
SstI GAGCTC 1 cut(s) 434
StuI AGGCCT 1 cut(s) 1023
StyD4I CCNGG 2 cut(s) 138, 237
TaaI ACNGT 2 cut(s) 194, 619
TaiI ACGT 2 cut(s) 273, 759
TaqI TCGA 6 cut(s) 35, 96, 173, 453, 465, 602
TasI AATT 4 cut(s) 85, 134, 908, 982
TatI WGTACW 1 cut(s) 856
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 6 cut(s) 69, 170, 185, 202, 212, 334
Tru1I TTAA 1 cut(s) 1013
Tru9I TTAA 1 cut(s) 1013
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 1 cut(s) 633
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 5 cut(s) 280, 429, 605, 843, 1046
TspGWI ACGGA 2 cut(s) 182, 665
TspRI CASTG 1 cut(s) 412
Tth111I GACNNNGTC 1 cut(s) 194
VpaK11BI GGWCC 2 cut(s) 57, 190
VspI ATTAAT 1 cut(s) 1013
XagI CCTNNNNNAGG 1 cut(s) 467
XapI RAATTY 3 cut(s) 134, 908, 982
XspI CTAG 3 cut(s) 339, 521, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.