Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012656

Physical Location & Seq

Genomic Coordinates Forward (+)

31978426 .. 31980868

Gene Structure 2443 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1230400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2094 bp

ATGGGTTGCTGCACCTACAAGTACTACTTCTTGTGGTTGACATGCTTGGCTTATCTTGTTCTTGTTCAAGGTAGTGAGAAGAAGCACCATTTTAATGGACCTGTTGTTAACGTGACGAAGCATCTCAATTTCCAAGATTTTAACTTTGTCAAAAACCCCAGAGTTTTGGAAGATGTAAGGCTCCTAGGCAGTGCCAAAATCTCAAGCTCATCGTCAGCCATACAAATCCCCGATGATCATGATCAGTACTCCGACGTCAACGGCGGCGATGATATCCATCATCTAAGACAGCAAGCAGGCCGAGCCCTCTACTCCTCTCCCATCCGACTCCTCGACCCTCACACTCAGTCTCCTGCTTCTTTTCAAACAACATTTTCTTTCCAATTCCACAACGGTTCTAATTTAAACGCTTCTGATCATGGTGGTAGTGGCCTCACCTTCATCATTGTCCCTGACGAGTTCACTGTCGGAAGGCCTGGCCCGTGGCTCGCCATGCTTAACGACGCTTGCGAGGATGATTACAAGGCGGTTGCAGTCGAGTTTGACACCCGCCTCAACCCTGAATTCGGAGACCCGAATGACAACCATGTTGGAATCAACTTGGGAACAATTGTCTCCACCGAAACAATCAACGCATCCGACGTTGGTGTATTTCTCAACGACGGCTCAGTCCACCGAGCGTGGATTAGCTACGATGGTCCGAACCGTTGGATGGAAATTCGTTTAGGATCAGAAGACAGTACTAATCACCCGCCACGAAAACCAATCTTCTCTGGCCCTCTGGATCTCTCACCATTTCTCAATGAGTACATGTTTGTCGGATTCTCTGCTTCAACGGGAAACCACACGCAAATCCACAGCGTCCTCTCGTGGAACTTCACCTCCACTAGTCAAGCTTTTCTCTGGATGCCGTCGATGGAAGCGTGCGAGAGTAACATAATTGTGACTGGCAGAGGAAGTAGTGCCGCGAGCGCTGAGCCTCGCGGGACTTTCTTAATCTTCATTGCTGTGGTGGTTTTGGCTCTGGCTGTGGTGGTTAGTCTGTACTATAGCGGCAGGCGCAAAACCACCGGAATATCAGACACTCCCGTTGTGTTGCCGGAGAAGACGCAGAGACCGAGGCCTCCGAACAAGCCTCGATACTTCACAATCTCCGAAATTTCCTTGGCTACTCAGTCCTTCAGCGAGTACGAAATTCTTGGTAGCGGCGAAAGAGGCGTGTACTATAGAGGGAAGCTTTTGAATGGTTGTCAAGTGGCTGTGAAGCGGTTTTCTTCTCTGTTTTTGAGCTCGGAAGGGCTAGATAGGCGGAGACTTTTCAAGGAAATAAAGGGGATTAGCCGGGTTAGGCATCCGAATCTGGTTCCAATCAGAGGGTGGTGTCAAGACAAGAAGGAAACCATGGTTGTGTATGAGTTTTTCCCCAATGGAAGCCTTGATAAATGGTTGTTTGGGGTGGGGGTCCTTCCGTGGACGCGGCGGTTCAAGGTTGTGAAAGATGTGGCTGACGCCCTCAGTTTCCTGCACTCCAAGCAATTATCCCACAAGAACTTGAAGACAACTAGTGTCTTTCTTGACGTCAGTTTCCGGGCTGTTTTAGGCGATTTCGGGTTTGTGCTAAGTGGTGCAGAATCAAGAAGGTTTGAGTCCGCAGTGAGCCAAAGTGCGGATGTTTTCGAGTTTGGTCTGTTTGTGTTGGAAGTGGTCGGTGGGAGGCCGCGATTGGAGGCGGATTCGGGGCCATTGGAGGAGAGGGATCTGTTGGATTTTGCATGGAGAATGCATGAGATTGATGAGATGGTGAGGGTAGTGGATAAGAGGATGGGCACTGTGATAAATTTGGAGCAAGCAATTAGGGTTATGCAAATAGGGTTGCTCTGCACTCTGAATGCGACCAAAGGTAGGCCTTGCATGGAGGAAGTGGTGGAGTTTCTTTGCATGGAGAGACAAATCCCCGAGTTGCCGCCGAGTCGGCCGGTTTCTCTCTTCCCTTACAGCAGCACCGGCGCCCTTTGCACTGGTTATTCCTTCAATATGTTTTGTAAGGCACACACAATTGCAATTGGCGCAAAATCGAGCACATTAATGTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002239 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0016020 GO:0043207 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Lectin_legB Pkinase_Tyr

Protein Analysis

698

Amino Acids

77.79

Weight (kDa)

6.56

Isoelectric Point (pI)

38.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	43 - 298	3.5e-54	Legume lectin domain
Lectin_L-type_dom	PF18483	121 - 287	5e-09	Legume lectin beta-barrel domain
Pkinase	PF00069	395 - 548	1.3e-19	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	398 - 539	1.2e-19	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012656)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33120
malus_domestica	MD01G1230400.v1.1
prunus_persica	Prupe.2G321100_v2.0.a1
pyrus_communis	pycom01g23840
rosa_chinensis	RchiOBHm_Chr1g0334661 RchiOBHm_Chr1g0383191
rosa_laevigata	RLG00000026124
rosa_multiflora	Rmu_sc0008563.1_g000003
rosa_roxburghii	Rroxscaffold_4G00277520
rosa_rugosa	Rorug01G0126300 Rorug01G0439400
rosa_samantha	Rh1AG139900 Rh1AG465000 Rh1BG422400 Rh1CG131800 Rh1CG435600 Rh1DG143800 Rh1DG455100
rosa_wichuraiana	Rw1G040750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	668
AatII	GACGTC	2 cut(s)	258, 1581
AccB1I	GGYRCC	1 cut(s)	2006
AccII	CGCG	4 cut(s)	968, 984, 1477, 1720
AclWI	GGATC	3 cut(s)	736, 792, 1764
AcoI	YGGCCR	1 cut(s)	1973
AcsI	RAATTY	5 cut(s)	563, 717, 1158, 1194, 1837
AcuI	CTGAAG	1 cut(s)	1165
AcyI	GRCGYC	4 cut(s)	255, 1509, 1578, 2007
AfaI	GTAC	7 cut(s)	23, 248, 742, 809, 1046, 1190, 1223
AfeI	AGCGCT	1 cut(s)	973
AfiI	CCNNNNNNNGG	5 cut(s)	566, 712, 1373, 1666, 1902
AflIII	ACRYGT	1 cut(s)	810
AgsI	TTSAA	8 cut(s)	68, 365, 834, 1243, 1321, 1486, 1555, 2032
AhlI	ACTAGT	2 cut(s)	887, 1562
AjnI	CCWGG	1 cut(s)	475
AloI	GAACNNNNNNTCC	2 cut(s)	866, 898
AluBI	AGCT	5 cut(s)	207, 690, 896, 1237, 1290
AluI	AGCT	5 cut(s)	207, 690, 896, 1237, 1290
Alw21I	GWGCWC	2 cut(s)	1292, 2081
Alw26I	GTCTC	6 cut(s)	354, 564, 619, 1108, 1306, 1939
AlwI	GGATC	3 cut(s)	736, 792, 1764
Ama87I	CYCGRG	1 cut(s)	1955
Aor51HI	AGCGCT	1 cut(s)	973
ApeKI	GCWGC	2 cut(s)	9, 1998
ApoI	RAATTY	5 cut(s)	563, 717, 1158, 1194, 1837
AseI	ATTAAT	1 cut(s)	2084
AspA2I	CCTAGG	1 cut(s)	184
AspLEI	GCGC	4 cut(s)	974, 1062, 2009, 2069
AspS9I	GGNCC	6 cut(s)	98, 479, 698, 776, 1462, 1739
AsuC2I	CCSGG	2 cut(s)	1343, 1589
AsuHPI	GGTGA	5 cut(s)	427, 740, 783, 871, 1813
AvaI	CYCGRG	1 cut(s)	1955
AvaII	GGWCC	3 cut(s)	98, 698, 1462
AvrII	CCTAGG	1 cut(s)	184
BaeGI	GKGCMC	1 cut(s)	1829
BanI	GGYRCC	1 cut(s)	2006
BanII	GRGCYC	2 cut(s)	307, 1292
BauI	CACGAG	1 cut(s)	868
BbsI	GAAGAC	3 cut(s)	741, 1112, 1562
Bbv12I	GWGCWC	2 cut(s)	1292, 2081
BbvI	GCAGC	1 cut(s)	2010
BccI	CCATC	7 cut(s)	285, 329, 689, 706, 910, 1792, 1816
BceAI	ACGGC	3 cut(s)	277, 679, 895
BciT130I	CCWGG	1 cut(s)	477
BclI	TGATCA	3 cut(s)	235, 241, 415
BcnI	CCSGG	2 cut(s)	1343, 1589
BcoDI	GTCTC	6 cut(s)	354, 564, 619, 1108, 1306, 1939
BcuI	ACTAGT	2 cut(s)	887, 1562
BfaI	CTAG	5 cut(s)	185, 888, 1301, 1563, 2092
BfmI	CTRYAG	2 cut(s)	1048, 1225
BfoI	RGCGCY	2 cut(s)	975, 2010
BglI	GCCNNNNNGGC	1 cut(s)	1972
BisI	GCNGC	9 cut(s)	10, 265, 966, 1054, 1207, 1478, 1718, 1964, 1999
BlnI	CCTAGG	1 cut(s)	184
BlpI	GCTNAGC	1 cut(s)	975
BlsI	GCNGC	9 cut(s)	11, 266, 967, 1055, 1208, 1479, 1719, 1965, 2000
BmcAI	AGTACT	3 cut(s)	23, 248, 742
Bme1390I	CCNGG	3 cut(s)	477, 1343, 1589
Bme18I	GGWCC	3 cut(s)	98, 698, 1462
BmeT110I	CYCGRG	1 cut(s)	1955
BmgT120I	GGNCC	6 cut(s)	98, 479, 698, 776, 1462, 1739
BmiI	GGNNCC	5 cut(s)	182, 1365, 1463, 1740, 2008
BmrFI	CCNGG	3 cut(s)	477, 1343, 1589
BmsI	GCATC	4 cut(s)	130, 644, 897, 1360
BpiI	GAAGAC	3 cut(s)	741, 1112, 1562
Bpu1102I	GCTNAGC	1 cut(s)	975
BpuEI	CTTGAG	1 cut(s)	187
BpuMI	CCSGG	2 cut(s)	1343, 1589
BsaBI	GATNNNNATC	2 cut(s)	240, 276
BsaHI	GRCGYC	4 cut(s)	255, 1509, 1578, 2007
BsaI	GGTCTC	2 cut(s)	564, 1108
BsaJI	CCNNGG	6 cut(s)	184, 482, 1118, 1164, 1401, 1469
BsaWI	WCCGGW	1 cut(s)	1070
BsaXI	ACNNNNNCTCC	4 cut(s)	334, 364, 866, 896
Bsc4I	CCNNNNNNNGG	5 cut(s)	566, 712, 1373, 1666, 1902
Bse118I	RCCGGY	2 cut(s)	1975, 2003
Bse1I	ACTGG	2 cut(s)	952, 2023
Bse3DI	GCAATG	1 cut(s)	1002
Bse8I	GATNNNNATC	2 cut(s)	240, 276
BseBI	CCWGG	1 cut(s)	477
BseDI	CCNNGG	6 cut(s)	184, 482, 1118, 1164, 1401, 1469
BseGI	GGATG	8 cut(s)	321, 520, 635, 717, 912, 1351, 1675, 1827
BseJI	GATNNNNATC	2 cut(s)	240, 276
BseLI	CCNNNNNNNGG	5 cut(s)	566, 712, 1373, 1666, 1902
BseMI	GCAATG	1 cut(s)	1002
BseMII	CTCAG	5 cut(s)	359, 681, 966, 1187, 1528
BseNI	ACTGG	2 cut(s)	952, 2023
BseRI	GAGGAG	3 cut(s)	304, 320, 1763
BseSI	GKGCMC	1 cut(s)	1829
BseX3I	CGGCCG	1 cut(s)	1973
BseXI	GCAGC	1 cut(s)	2010
BsgI	GTGCAG	3 cut(s)	1508, 1647, 1864
Bsh1236I	CGCG	4 cut(s)	968, 984, 1477, 1720
Bsh1285I	CGRYCG	1 cut(s)	1976
BshNI	GGYRCC	1 cut(s)	2006
BsiEI	CGRYCG	1 cut(s)	1976
BsiHKAI	GWGCWC	2 cut(s)	1292, 2081
BsiHKCI	CYCGRG	1 cut(s)	1955
BsiSI	CCGG	6 cut(s)	1071, 1100, 1342, 1588, 1976, 2004
BslFI	GGGAC	2 cut(s)	434, 1000
BslI	CCNNNNNNNGG	5 cut(s)	566, 712, 1373, 1666, 1902
BsmAI	GTCTC	6 cut(s)	354, 564, 619, 1108, 1306, 1939
BsmFI	GGGAC	2 cut(s)	434, 1000
BsmI	GAATGC	2 cut(s)	1785, 1894
Bso31I	GGTCTC	2 cut(s)	564, 1108
BsoBI	CYCGRG	1 cut(s)	1955
Bsp1286I	GDGCHC	4 cut(s)	307, 1292, 1829, 2081
Bsp143I	GATC	6 cut(s)	235, 241, 415, 728, 784, 1756
Bsp1720I	GCTNAGC	1 cut(s)	975
Bsp19I	CCATGG	1 cut(s)	1401
BspCNI	CTCAG	5 cut(s)	358, 680, 967, 1186, 1527
BspFNI	CGCG	4 cut(s)	968, 984, 1477, 1720
BspHI	TCATGA	1 cut(s)	238
BspLI	GGNNCC	5 cut(s)	182, 1365, 1463, 1740, 2008
BspPI	GGATC	3 cut(s)	736, 792, 1764
BspT107I	GGYRCC	1 cut(s)	2006
BspTNI	GGTCTC	2 cut(s)	564, 1108
BsrDI	GCAATG	1 cut(s)	1002
BsrFI	RCCGGY	2 cut(s)	1975, 2003
BsrI	ACTGG	2 cut(s)	952, 2023
BssAI	RCCGGY	2 cut(s)	1975, 2003
BssECI	CCNNGG	6 cut(s)	184, 482, 1118, 1164, 1401, 1469
BssMI	GATC	6 cut(s)	235, 241, 415, 728, 784, 1756
BssNI	GRCGYC	4 cut(s)	255, 1509, 1578, 2007
BssSI	CACGAG	1 cut(s)	868
BssT1I	CCWWGG	3 cut(s)	184, 1164, 1401
Bst2BI	CACGAG	1 cut(s)	868
Bst2UI	CCWGG	1 cut(s)	477
Bst4CI	ACNGT	5 cut(s)	395, 466, 707, 740, 1831
Bst6I	CTCTTC	1 cut(s)	1991
BstACI	GRCGYC	4 cut(s)	255, 1509, 1578, 2007
BstC8I	GCNNGC	8 cut(s)	294, 298, 489, 508, 925, 970, 1058, 1848
BstDEI	CTNAG	7 cut(s)	284, 345, 667, 975, 1173, 1514, 1619
BstDSI	CCRYGG	3 cut(s)	482, 1401, 1469
BstF5I	GGATG	8 cut(s)	321, 520, 635, 717, 912, 1351, 1675, 1827
BstFNI	CGCG	4 cut(s)	968, 984, 1477, 1720
BstH2I	RGCGCY	2 cut(s)	975, 2010
BstHHI	GCGC	4 cut(s)	974, 1062, 2009, 2069
BstKTI	GATC	6 cut(s)	238, 244, 418, 731, 787, 1759
BstMAI	GTCTC	6 cut(s)	354, 564, 619, 1108, 1306, 1939
BstMBI	GATC	6 cut(s)	235, 241, 415, 728, 784, 1756
BstMCI	CGRYCG	1 cut(s)	1976
BstNI	CCWGG	1 cut(s)	477
BstNSI	RCATGY	2 cut(s)	45, 814
BstSCI	CCNGG	3 cut(s)	475, 1341, 1587
BstSFI	CTRYAG	2 cut(s)	1048, 1225
BstSLI	GKGCMC	1 cut(s)	1829
BstUI	CGCG	4 cut(s)	968, 984, 1477, 1720
BstV1I	GCAGC	1 cut(s)	2010
BstV2I	GAAGAC	3 cut(s)	741, 1112, 1562
BstX2I	RGATCY	2 cut(s)	784, 1756
BstXI	CCANNNNNNTGG	2 cut(s)	95, 166
BstYI	RGATCY	2 cut(s)	784, 1756
BstZI	CGGCCG	1 cut(s)	1973
BtgI	CCRYGG	3 cut(s)	482, 1401, 1469
BtgZI	GCGATG	1 cut(s)	282
BtsCI	GGATG	8 cut(s)	321, 520, 635, 717, 912, 1351, 1675, 1827
BtsI	GCAGTG	2 cut(s)	196, 1659
BtsIMutI	CAGTG	5 cut(s)	196, 462, 1659, 1827, 2016
Cac8I	GCNNGC	8 cut(s)	294, 298, 489, 508, 925, 970, 1058, 1848
CciI	TCATGA	1 cut(s)	238
CfoI	GCGC	4 cut(s)	974, 1062, 2009, 2069
Cfr10I	RCCGGY	2 cut(s)	1975, 2003
Cfr13I	GGNCC	6 cut(s)	98, 479, 698, 776, 1462, 1739
CseI	GACGC	5 cut(s)	512, 850, 1117, 1483, 1517
Csp6I	GTAC	7 cut(s)	22, 247, 741, 808, 1045, 1189, 1222
CviQI	GTAC	7 cut(s)	22, 247, 741, 808, 1045, 1189, 1222
DdeI	CTNAG	7 cut(s)	284, 345, 667, 975, 1173, 1514, 1619
DinI	GGCGCC	1 cut(s)	2008
DpnI	GATC	6 cut(s)	237, 243, 417, 730, 786, 1758
DpnII	GATC	6 cut(s)	235, 241, 415, 728, 784, 1756
DraI	TTTAAA	1 cut(s)	405
DrdI	GACNNNNNNGTC	1 cut(s)	668
DseDI	GACNNNNNNGTC	1 cut(s)	668
EaeI	YGGCCR	1 cut(s)	1973
EagI	CGGCCG	1 cut(s)	1973
Eam1104I	CTCTTC	1 cut(s)	1991
EarI	CTCTTC	1 cut(s)	1991
EciI	GGCGGA	2 cut(s)	1324, 1745
Ecl136II	GAGCTC	1 cut(s)	1290
EclXI	CGGCCG	1 cut(s)	1973
Eco130I	CCWWGG	3 cut(s)	184, 1164, 1401
Eco147I	AGGCCT	3 cut(s)	475, 1123, 1906
Eco24I	GRGCYC	2 cut(s)	307, 1292
Eco31I	GGTCTC	2 cut(s)	564, 1108
Eco32I	GATATC	1 cut(s)	274
Eco47I	GGWCC	3 cut(s)	98, 698, 1462
Eco47III	AGCGCT	1 cut(s)	973
Eco52I	CGGCCG	1 cut(s)	1973
Eco53kI	GAGCTC	1 cut(s)	1290
Eco57I	CTGAAG	1 cut(s)	1165
Eco88I	CYCGRG	1 cut(s)	1955
EcoICRI	GAGCTC	1 cut(s)	1290
EcoO109I	RGGNCCY	1 cut(s)	1462
EcoRI	GAATTC	1 cut(s)	563
EcoRII	CCWGG	1 cut(s)	475
EcoRV	GATATC	1 cut(s)	274
EcoT14I	CCWWGG	3 cut(s)	184, 1164, 1401
EcoT22I	ATGCAT	1 cut(s)	1785
EcoT38I	GRGCYC	2 cut(s)	307, 1292
EgeI	GGCGCC	1 cut(s)	2008
EheI	GGCGCC	1 cut(s)	2008
ErhI	CCWWGG	3 cut(s)	184, 1164, 1401
FalI	AAGNNNNNCTT	2 cut(s)	11, 43
FaqI	GGGAC	2 cut(s)	434, 1000
FauI	CCCGC	3 cut(s)	557, 759, 977
FbaI	TGATCA	3 cut(s)	235, 241, 415
Fnu4HI	GCNGC	9 cut(s)	10, 265, 966, 1054, 1207, 1478, 1718, 1964, 1999
FokI	GGATG	8 cut(s)	308, 527, 622, 724, 919, 1338, 1682, 1834
FriOI	GRGCYC	2 cut(s)	307, 1292
Fsp4HI	GCNGC	9 cut(s)	10, 265, 966, 1054, 1207, 1478, 1718, 1964, 1999
FspBI	CTAG	5 cut(s)	185, 888, 1301, 1563, 2092
GlaI	GCGC	4 cut(s)	973, 1061, 2008, 2068
GluI	GCNGC	9 cut(s)	10, 265, 966, 1054, 1207, 1478, 1718, 1964, 1999
HaeII	RGCGCY	2 cut(s)	975, 2010
HapII	CCGG	6 cut(s)	1071, 1100, 1342, 1588, 1976, 2004
HgaI	GACGC	5 cut(s)	512, 850, 1117, 1483, 1517
HhaI	GCGC	4 cut(s)	974, 1062, 2009, 2069
Hin1I	GRCGYC	4 cut(s)	255, 1509, 1578, 2007
Hin6I	GCGC	4 cut(s)	972, 1060, 2007, 2067
HinP1I	GCGC	4 cut(s)	972, 1060, 2007, 2067
HincII	GTYRAC	3 cut(s)	39, 109, 259
HindII	GTYRAC	3 cut(s)	39, 109, 259
HindIII	AAGCTT	2 cut(s)	894, 1235
HinfI	GANTC	8 cut(s)	327, 594, 822, 1357, 1631, 1646, 1733, 1969
HpaI	GTTAAC	1 cut(s)	109
HpaII	CCGG	6 cut(s)	1071, 1100, 1342, 1588, 1976, 2004
HphI	GGTGA	5 cut(s)	427, 740, 783, 871, 1813
Hpy166II	GTNNAC	7 cut(s)	39, 109, 259, 462, 673, 1222, 1473
Hpy188III	TCNNGA	6 cut(s)	239, 782, 904, 1385, 1574, 1635
Hpy8I	GTNNAC	7 cut(s)	39, 109, 259, 462, 673, 1222, 1473
Hpy99I	CGWCG	5 cut(s)	257, 506, 644, 665, 916
HpyAV	CCTTC	8 cut(s)	448, 465, 1189, 1289, 1387, 1475, 1632, 2038
HpyCH4III	ACNGT	5 cut(s)	395, 466, 707, 740, 1831
HpyCH4IV	ACGT	4 cut(s)	111, 255, 642, 1578
HpyF3I	CTNAG	7 cut(s)	284, 345, 667, 975, 1173, 1514, 1619
HpySE526I	ACGT	4 cut(s)	111, 255, 642, 1578
Hsp92I	GRCGYC	4 cut(s)	255, 1509, 1578, 2007
HspAI	GCGC	4 cut(s)	972, 1060, 2007, 2067
KasI	GGCGCC	1 cut(s)	2006
Ksp22I	TGATCA	3 cut(s)	235, 241, 415
KspAI	GTTAAC	1 cut(s)	109
Kzo9I	GATC	6 cut(s)	235, 241, 415, 728, 784, 1756
LmnI	GCTCC	2 cut(s)	186, 1843
Lsp1109I	GCAGC	1 cut(s)	2010
LweI	GCATC	4 cut(s)	130, 644, 897, 1360
MaeI	CTAG	5 cut(s)	185, 888, 1301, 1563, 2092
MaeII	ACGT	4 cut(s)	111, 255, 642, 1578
MaeIII	GTNAC	3 cut(s)	112, 932, 943
MalI	GATC	6 cut(s)	237, 243, 417, 730, 786, 1758
MboI	GATC	6 cut(s)	235, 241, 415, 728, 784, 1756
MboII	GAAGA	9 cut(s)	91, 182, 746, 760, 991, 1117, 1266, 1567, 1978
MfeI	CAATTG	3 cut(s)	609, 2055, 2061
MflI	RGATCY	2 cut(s)	784, 1756
MhlI	GDGCHC	4 cut(s)	307, 1292, 1829, 2081
Mly113I	GGCGCC	1 cut(s)	2007
MlyI	GAGTC	3 cut(s)	321, 1655, 1978
MmeI	TCCRAC	9 cut(s)	276, 349, 448, 571, 663, 689, 799, 1677, 1743
Mph1103I	ATGCAT	1 cut(s)	1785
MseI	TTAA	7 cut(s)	93, 108, 141, 404, 498, 995, 2084
MslI	CAYNNNNRTG	5 cut(s)	93, 941, 1007, 1406, 2084
MspI	CCGG	6 cut(s)	1071, 1100, 1342, 1588, 1976, 2004
MspR9I	CCNGG	3 cut(s)	477, 1343, 1589
MunI	CAATTG	3 cut(s)	609, 2055, 2061
Mva1269I	GAATGC	2 cut(s)	1785, 1894
MvaI	CCWGG	1 cut(s)	477
MvnI	CGCG	4 cut(s)	968, 984, 1477, 1720
NarI	GGCGCC	1 cut(s)	2007
NciI	CCSGG	2 cut(s)	1343, 1589
NcoI	CCATGG	1 cut(s)	1401
NdeII	GATC	6 cut(s)	235, 241, 415, 728, 784, 1756
NlaIV	GGNNCC	5 cut(s)	182, 1365, 1463, 1740, 2008
NmeAIII	GCCGAG	2 cut(s)	326, 1992
NmuCI	GTSAC	2 cut(s)	112, 943
NsiI	ATGCAT	1 cut(s)	1785
NspI	RCATGY	2 cut(s)	45, 814
PagI	TCATGA	1 cut(s)	238
PceI	AGGCCT	3 cut(s)	475, 1123, 1906
PciI	ACATGT	1 cut(s)	810
PcsI	WCGNNNNNNNCGW	4 cut(s)	507, 639, 920, 1115
PctI	GAATGC	2 cut(s)	1785, 1894
PfeI	GAWTC	5 cut(s)	594, 822, 1357, 1631, 1733
PkrI	GCNGC	9 cut(s)	11, 266, 967, 1055, 1208, 1479, 1719, 1965, 2000
PleI	GAGTC	3 cut(s)	321, 1654, 1977
PluTI	GGCGCC	1 cut(s)	2010
PpsI	GAGTC	3 cut(s)	321, 1654, 1977
PpuMI	RGGWCCY	1 cut(s)	1462
PscI	ACATGT	1 cut(s)	810
PshBI	ATTAAT	1 cut(s)	2084
Psp124BI	GAGCTC	1 cut(s)	1292
Psp5II	RGGWCCY	1 cut(s)	1462
Psp6I	CCWGG	1 cut(s)	475
PspGI	CCWGG	1 cut(s)	475
PspN4I	GGNNCC	5 cut(s)	182, 1365, 1463, 1740, 2008
PspPI	GGNCC	6 cut(s)	98, 479, 698, 776, 1462, 1739
PspPPI	RGGWCCY	1 cut(s)	1462
PsuI	RGATCY	2 cut(s)	784, 1756
RsaI	GTAC	7 cut(s)	23, 248, 742, 809, 1046, 1190, 1223
RsaNI	GTAC	7 cut(s)	22, 247, 741, 808, 1045, 1189, 1222
RseI	CAYNNNNRTG	5 cut(s)	93, 941, 1007, 1406, 2084
SacI	GAGCTC	1 cut(s)	1292
SaqAI	TTAA	7 cut(s)	93, 108, 141, 404, 498, 995, 2084
SatI	GCNGC	9 cut(s)	10, 265, 966, 1054, 1207, 1478, 1718, 1964, 1999
Sau3AI	GATC	6 cut(s)	235, 241, 415, 728, 784, 1756
Sau96I	GGNCC	6 cut(s)	98, 479, 698, 776, 1462, 1739
ScaI	AGTACT	3 cut(s)	23, 248, 742
SchI	GAGTC	3 cut(s)	321, 1655, 1978
ScrFI	CCNGG	3 cut(s)	477, 1343, 1589
SduI	GDGCHC	4 cut(s)	307, 1292, 1829, 2081
SfaNI	GCATC	4 cut(s)	130, 644, 897, 1360
SfcI	CTRYAG	2 cut(s)	1048, 1225
SfoI	GGCGCC	1 cut(s)	2008
SgrAI	CRCCGGYG	1 cut(s)	2003
SinI	GGWCC	3 cut(s)	98, 698, 1462
SmiMI	CAYNNNNRTG	5 cut(s)	93, 941, 1007, 1406, 2084
SmlI	CTYRAG	1 cut(s)	202
SmoI	CTYRAG	1 cut(s)	202
SpeI	ACTAGT	2 cut(s)	887, 1562
SseBI	AGGCCT	3 cut(s)	475, 1123, 1906
SspDI	GGCGCC	1 cut(s)	2006
SspMI	CTAG	5 cut(s)	185, 888, 1301, 1563, 2092
SstI	GAGCTC	1 cut(s)	1292
StuI	AGGCCT	3 cut(s)	475, 1123, 1906
StyD4I	CCNGG	3 cut(s)	475, 1341, 1587
StyI	CCWWGG	3 cut(s)	184, 1164, 1401
TaaI	ACNGT	5 cut(s)	395, 466, 707, 740, 1831
TaiI	ACGT	4 cut(s)	114, 258, 645, 1581
TaqI	TCGA	6 cut(s)	333, 537, 914, 1138, 1677, 2075
TaqII	GACCGA	1 cut(s)	1132
TatI	WGTACW	6 cut(s)	21, 246, 740, 807, 1044, 1221
TauI	GCSGC	7 cut(s)	267, 968, 1056, 1209, 1480, 1720, 1966
TfiI	GAWTC	5 cut(s)	594, 822, 1357, 1631, 1733
Tru1I	TTAA	7 cut(s)	93, 108, 141, 404, 498, 995, 2084
Tru9I	TTAA	7 cut(s)	93, 108, 141, 404, 498, 995, 2084
TscAI	CASTG	5 cut(s)	196, 469, 1659, 1834, 2023
TseFI	GTSAC	2 cut(s)	112, 943
TseI	GCWGC	2 cut(s)	9, 1998
Tsp45I	GTSAC	2 cut(s)	112, 943
TspDTI	ATGAA	2 cut(s)	430, 991
TspGWI	ACGGA	1 cut(s)	1458
TspRI	CASTG	5 cut(s)	196, 469, 1659, 1834, 2023
VpaK11BI	GGWCC	3 cut(s)	98, 698, 1462
VspI	ATTAAT	1 cut(s)	2084
XapI	RAATTY	5 cut(s)	563, 717, 1158, 1194, 1837
XceI	RCATGY	2 cut(s)	45, 814
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1374
XmaJI	CCTAGG	1 cut(s)	184
XspI	CTAG	5 cut(s)	185, 888, 1301, 1563, 2092
ZraI	GACGTC	2 cut(s)	256, 1579
ZrmI	AGTACT	3 cut(s)	23, 248, 742
Zsp2I	ATGCAT	1 cut(s)	1785

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.