Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0012656

Physical Location & Seq

Genomic Coordinates Forward (+)

68045811 .. 68048456

Gene Structure 2646 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG465000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGAGTTGCTTCAACTGGTTGCTCTATTTAGCTGCTGCTTTGGCCGTAATACGCAACGAGCTTCTAGTTGATTCAGCTAGTGACAAGAAGCACCACAGCGGACCAGTTGTTAATGTGACTAAGCACCTCATGTTCCCAGATTTCAACCTCTCCTACAACCCCCGAATCTTGAACGATGTAAAGCTCTTAGGCAGTGCCAAGCTTGCTTCAAAAACCAGTGCTATACAAATCCCCGACGAGTCTTATGTCACTGATCTCAGACACCAAGCCGGCCGAGCCATCTACTCTTCTTCAATCCGACTCCTAGACCCTCACACCGAAACCCCTGCCTCTTTCGAAACAACCTTCTCTTTTCAATTCCACAATGCCTCTTCATCTTCTCCTGAACGAATCCAAACTACTCAAAGCGGTGGTTATGGTAATGGTGGCAGTGGACTAGCCTTCGTCATCGTCCCGGATGAGTTCACAGTTGGCAGACCTGGTGCTTGGCTTGCCATGCTCAACGATGCTTGTGAAGATGACTACAAGGTCATAGCAATCGAATTCGACACACGTTTGAATCCAGAGTTTGGAGACCCTAATGATAACCATGTAGGTATCAACTTGGGGACTATTATCTCCACAGAAACAATCAACGCTTCTGATGTTGGTGTCTTCCTCAACGACGGTTCAGTCCACCGAGCTTGGATCAGCTACAACGGCTCACGCCGTTGGATGGAAATTCGTTTGGGATCAGACGACGGAAGTTACCCTTCAAAGCCAGTCTTCTCCGGATCTCTTGACCTCTCACCGTTTCTGAATGAGTACATGTTCGTTGGATTTTCGGCTTCCACAGGCAATCACACCCAAATCCACAGCGTGCTTTCTTGGAACTTCACTTCTGTTAGTCAAGCTTTTCTTCGTGTGCCGTCAATGGAGACATGTGAGAGCAAGATTGTTGTTACTGAAAACTCTCGTGCTGAGCCTCCCGGCACGTTCTTGATCTTTATAGCTGTGCTGGCACTTGCTATAGCTGTTATGCTTAGCCTTTACTATAACAGCAAGCGTAGAAGTGCAAATTCGGATAGTGCTAGTATAGTGTTGCCGGAGAAGAAGCAGAGACCGAGGCCTCCAAACAAGCCTAGAAGCTTCACAATCTCGGAGATTTCATTGGCGACTAGGTCTTTCGGAGAGCTAGAGATACTTGGAGGTGGATCAAAAGGGGTGTGCTACCGAGGGAAGCTTTCGAACGGATGTCAAGTGGCAGTGAAGCGGTTTTCGCCTCAGTTTCTGAACTCACAAGGGTTGGATAGGAGAAGATTGATGAAGGAAATCAAGGGGATTAGCAGGGTTAGACACCCGAACTTAGTTTCCATTAGAGGATGGTGTCAGGACAAGAAAGAGACTATGGTTGTGTATGAGTTTTTCTCCAATGGAAGCCTTGATAAGTGGCTGTTTGGTGTTGGTGTTCTTCCGTGGACTCGGAGGTTCAAGGTTGTGAGAGATGTGGCTGAAGCACTCAGTTACTTGCACTCCAATCAATTGGCGCACAAGAACTTGAAGACCACCAATGTCTTTCTTGATGTTAGTTTCCGGGCTGTTCTAGGAGACTTTGGGTTTGTGCTATGTGGAGCAGAGTCGAGAAGGTTTGAATCGATGGTGAGTCAAAGCGCCGATGTGTTTGAATTCGGCCTCTTTGTGTTGAAAGTGGTGGGAGGGAGGAAGAGGTTGGAGGCAGAGTTGGGTGAATTGGAGGAGAGGGACTTGCTGGACTTCACCTGGAGAATGCATGAAATTGGTGAAATGAGGAGGGTTGTGGATAAGAGAATGGGTGCAGTGATCAATTTGGATCAAGCAATTAGGGTTTTGGAGATTGGATTGCTTTGCACACTGAATGCGAGCAAAGGGAGGCCTAGTATGGAGCAAGTTGTGGAGTTTCTTTGCATGGAGAGGCCAATCCCTGAGTTGCCACAGACTCGACCTGTTGCTTTGTTCCCCTACAACAGCACCAATGCTCTCTGTACAGGTTATTCTTGTGCTCCTTTCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002239 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0016020 GO:0043207 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Lectin_legB Pkinase_Tyr

Protein Analysis

676

Amino Acids

75.24

Weight (kDa)

8.37

Isoelectric Point (pI)

41.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	58 - 296	2.1e-51	Legume lectin domain
Lectin_L-type_dom	PF18483	63 - 286	5.4e-09	Legume lectin beta-barrel domain
Pkinase	PF00069	391 - 541	5.9e-24	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	393 - 533	1.5e-22	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012656)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33120
malus_domestica	MD01G1230400.v1.1
prunus_persica	Prupe.2G321100_v2.0.a1
pyrus_communis	pycom01g23840
rosa_chinensis	RchiOBHm_Chr1g0334661 RchiOBHm_Chr1g0383191
rosa_laevigata	RLG00000026124
rosa_multiflora	Rmu_sc0008563.1_g000003
rosa_roxburghii	Rroxscaffold_4G00277520
rosa_rugosa	Rorug01G0126300 Rorug01G0439400
rosa_samantha	Rh1AG139900 Rh1AG465000 Rh1BG422400 Rh1CG131800 Rh1CG435600 Rh1DG143800 Rh1DG455100
rosa_wichuraiana	Rw1G040750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	527, 671
AccB7I	CCANNNNNTGG	1 cut(s)	569
AccIII	TCCGGA	1 cut(s)	770
AciI	CCGC	3 cut(s)	99, 408, 1252
AclWI	GGATC	5 cut(s)	695, 739, 781, 1201, 1836
AcoI	YGGCCR	2 cut(s)	42, 271
AcsI	RAATTY	4 cut(s)	542, 720, 1057, 1664
AcuI	CTGAAG	1 cut(s)	1512
AdeI	CACNNNGTG	1 cut(s)	859
AfaI	GTAC	2 cut(s)	806, 2002
AfiI	CCNNNNNNNGG	2 cut(s)	569, 715
AflIII	ACRYGT	3 cut(s)	551, 807, 920
AjnI	CCWGG	2 cut(s)	478, 1757
AloI	GAACNNNNNNTCC	2 cut(s)	1334, 1366
Alw21I	GWGCWC	1 cut(s)	2020
Alw26I	GTCTC	5 cut(s)	567, 911, 1093, 1376, 1581
AlwI	GGATC	5 cut(s)	695, 739, 781, 1201, 1836
AlwNI	CAGNNNCTG	1 cut(s)	1270
Aor13HI	TCCGGA	1 cut(s)	770
AoxI	GGCC	6 cut(s)	42, 271, 1106, 1669, 1889, 1931
ApeKI	GCWGC	2 cut(s)	32, 35
ApoI	RAATTY	4 cut(s)	542, 720, 1057, 1664
ArsI	GACNNNNNNTTYG	2 cut(s)	1580, 1612
AspLEI	GCGC	2 cut(s)	1528, 1652
AspS9I	GGNCC	1 cut(s)	101
AsuC2I	CCSGG	3 cut(s)	455, 969, 1574
AsuHPI	GGTGA	5 cut(s)	780, 1651, 1736, 1747, 1790
AsuII	TTCGAA	2 cut(s)	336, 1226
AvaII	GGWCC	1 cut(s)	101
BauI	CACGAG	1 cut(s)	954
BbsI	GAAGAC	3 cut(s)	646, 757, 1547
Bbv12I	GWGCWC	1 cut(s)	2020
BbvI	GCAGC	2 cut(s)	19, 22
BccI	CCATC	4 cut(s)	287, 709, 1356, 1630
BceAI	ACGGC	4 cut(s)	29, 693, 715, 892
BcgI	CGANNNNNNTGC	2 cut(s)	308, 342
BciT130I	CCWGG	2 cut(s)	480, 1759
BclI	TGATCA	1 cut(s)	1818
BcnI	CCSGG	3 cut(s)	455, 969, 1574
BcoDI	GTCTC	5 cut(s)	567, 911, 1093, 1376, 1581
BfmI	CTRYAG	1 cut(s)	1008
BfoI	RGCGCY	1 cut(s)	1653
BisI	GCNGC	2 cut(s)	33, 36
BlpI	GCTNAGC	2 cut(s)	960, 1022
BlsI	GCNGC	2 cut(s)	34, 37
Bme1390I	CCNGG	5 cut(s)	455, 480, 969, 1574, 1759
Bme18I	GGWCC	1 cut(s)	101
BmgT120I	GGNCC	1 cut(s)	101
BmrFI	CCNGG	5 cut(s)	455, 480, 969, 1574, 1759
BmsI	GCATC	1 cut(s)	496
BpiI	GAAGAC	3 cut(s)	646, 757, 1547
BplI	GAGNNNNNCTC	2 cut(s)	1391, 1423
BpmI	CTGGAG	1 cut(s)	1780
Bpu1102I	GCTNAGC	2 cut(s)	960, 1022
Bpu14I	TTCGAA	2 cut(s)	336, 1226
BpuMI	CCSGG	3 cut(s)	455, 969, 1574
Bsa29I	ATCGAT	1 cut(s)	1634
BsaI	GGTCTC	2 cut(s)	567, 1093
BsaJI	CCNNGG	3 cut(s)	1103, 1213, 1454
BsaWI	WCCGGW	1 cut(s)	770
BsaXI	ACNNNNNCTCC	6 cut(s)	1496, 1526, 1703, 1733, 1879, 1909
Bsc4I	CCNNNNNNNGG	2 cut(s)	569, 715
Bse118I	RCCGGY	1 cut(s)	269
Bse1I	ACTGG	4 cut(s)	20, 104, 216, 761
BseAI	TCCGGA	1 cut(s)	770
BseBI	CCWGG	2 cut(s)	480, 1759
BseCI	ATCGAT	1 cut(s)	1634
BseDI	CCNNGG	3 cut(s)	1103, 1213, 1454
BseGI	GGATG	4 cut(s)	463, 720, 1238, 1367
BseLI	CCNNNNNNNGG	2 cut(s)	569, 715
BseMII	CTCAG	5 cut(s)	271, 951, 1277, 1513, 1932
BseNI	ACTGG	4 cut(s)	20, 104, 216, 761
BseRI	GAGGAG	2 cut(s)	1748, 1801
BseX3I	CGGCCG	1 cut(s)	271
BseXI	GCAGC	2 cut(s)	19, 22
BsgI	GTGCAG	1 cut(s)	1833
Bsh1285I	CGRYCG	1 cut(s)	274
BshFI	GGCC	6 cut(s)	44, 273, 1108, 1671, 1891, 1933
BshVI	ATCGAT	1 cut(s)	1634
BsiEI	CGRYCG	1 cut(s)	274
BsiHKAI	GWGCWC	1 cut(s)	2020
BsiSI	CCGG	6 cut(s)	270, 455, 771, 969, 1085, 1573
BslFI	GGGAC	3 cut(s)	437, 622, 1754
BslI	CCNNNNNNNGG	2 cut(s)	569, 715
BsmAI	GTCTC	5 cut(s)	567, 911, 1093, 1376, 1581
BsmFI	GGGAC	3 cut(s)	437, 622, 1754
BsmI	GAATGC	2 cut(s)	1770, 1879
BsnI	GGCC	6 cut(s)	44, 273, 1108, 1671, 1891, 1933
Bso31I	GGTCTC	2 cut(s)	567, 1093
Bsp119I	TTCGAA	2 cut(s)	336, 1226
Bsp1286I	GDGCHC	1 cut(s)	2020
Bsp13I	TCCGGA	1 cut(s)	770
Bsp1407I	TGTACA	1 cut(s)	2000
Bsp143I	GATC	8 cut(s)	253, 687, 731, 773, 981, 1193, 1818, 1828
Bsp1720I	GCTNAGC	2 cut(s)	960, 1022
BspACI	CCGC	3 cut(s)	99, 408, 1252
BspANI	GGCC	6 cut(s)	44, 273, 1108, 1671, 1891, 1933
BspCNI	CTCAG	5 cut(s)	270, 952, 1276, 1512, 1933
BspDI	ATCGAT	1 cut(s)	1634
BspEI	TCCGGA	1 cut(s)	770
BspPI	GGATC	5 cut(s)	695, 739, 781, 1201, 1836
BspT104I	TTCGAA	2 cut(s)	336, 1226
BspTNI	GGTCTC	2 cut(s)	567, 1093
BsrFI	RCCGGY	1 cut(s)	269
BsrGI	TGTACA	1 cut(s)	2000
BsrI	ACTGG	4 cut(s)	20, 104, 216, 761
BssAI	RCCGGY	1 cut(s)	269
BssECI	CCNNGG	3 cut(s)	1103, 1213, 1454
BssMI	GATC	8 cut(s)	253, 687, 731, 773, 981, 1193, 1818, 1828
BssSI	CACGAG	1 cut(s)	954
Bst2BI	CACGAG	1 cut(s)	954
Bst2UI	CCWGG	2 cut(s)	480, 1759
Bst4CI	ACNGT	3 cut(s)	469, 668, 792
Bst6I	CTCTTC	3 cut(s)	292, 376, 1697
BstAUI	TGTACA	1 cut(s)	2000
BstBI	TTCGAA	2 cut(s)	336, 1226
BstC8I	GCNNGC	7 cut(s)	204, 271, 492, 860, 999, 1043, 1879
BstDEI	CTNAG	9 cut(s)	120, 187, 257, 960, 1022, 1263, 1345, 1499, 1941
BstDSI	CCRYGG	1 cut(s)	1454
BstF5I	GGATG	4 cut(s)	463, 720, 1238, 1367
BstH2I	RGCGCY	1 cut(s)	1653
BstHHI	GCGC	2 cut(s)	1528, 1652
BstKTI	GATC	8 cut(s)	256, 690, 734, 776, 984, 1196, 1821, 1831
BstMAI	GTCTC	5 cut(s)	567, 911, 1093, 1376, 1581
BstMBI	GATC	8 cut(s)	253, 687, 731, 773, 981, 1193, 1818, 1828
BstMCI	CGRYCG	1 cut(s)	274
BstMWI	GCNNNNNNNGC	8 cut(s)	41, 203, 275, 491, 496, 699, 998, 1258
BstNI	CCWGG	2 cut(s)	480, 1759
BstNSI	RCATGY	2 cut(s)	811, 924
BstSCI	CCNGG	5 cut(s)	453, 478, 967, 1572, 1757
BstSFI	CTRYAG	1 cut(s)	1008
BstV1I	GCAGC	2 cut(s)	19, 22
BstV2I	GAAGAC	3 cut(s)	646, 757, 1547
BstX2I	RGATCY	1 cut(s)	773
BstXI	CCANNNNNNTGG	1 cut(s)	1522
BstYI	RGATCY	1 cut(s)	773
BstZI	CGGCCG	1 cut(s)	271
Bsu15I	ATCGAT	1 cut(s)	1634
BsuRI	GGCC	6 cut(s)	44, 273, 1108, 1671, 1891, 1933
BsuTUI	ATCGAT	1 cut(s)	1634
BtgI	CCRYGG	1 cut(s)	1454
BtsCI	GGATG	4 cut(s)	463, 720, 1238, 1367
BtsI	GCAGTG	4 cut(s)	199, 436, 1251, 1821
BtsIMutI	CAGTG	7 cut(s)	199, 223, 249, 436, 1251, 1821, 1868
Cac8I	GCNNGC	7 cut(s)	204, 271, 492, 860, 999, 1043, 1879
CaiI	CAGNNNCTG	1 cut(s)	1270
CfoI	GCGC	2 cut(s)	1528, 1652
Cfr10I	RCCGGY	1 cut(s)	269
Cfr13I	GGNCC	1 cut(s)	101
ClaI	ATCGAT	1 cut(s)	1634
CsiI	ACCWGGT	1 cut(s)	478
Csp6I	GTAC	2 cut(s)	805, 2001
CspCI	CAANNNNNGTGG	2 cut(s)	665, 700
CviAII	CATG	7 cut(s)	130, 496, 590, 808, 921, 1769, 1924
CviQI	GTAC	2 cut(s)	805, 2001
DdeI	CTNAG	9 cut(s)	120, 187, 257, 960, 1022, 1263, 1345, 1499, 1941
DpnI	GATC	8 cut(s)	255, 689, 733, 775, 983, 1195, 1820, 1830
DpnII	GATC	8 cut(s)	253, 687, 731, 773, 981, 1193, 1818, 1828
DraIII	CACNNNGTG	1 cut(s)	859
DrdI	GACNNNNNNGTC	2 cut(s)	527, 671
DseDI	GACNNNNNNGTC	2 cut(s)	527, 671
EaeI	YGGCCR	2 cut(s)	42, 271
EagI	CGGCCG	1 cut(s)	271
Eam1104I	CTCTTC	3 cut(s)	292, 376, 1697
EarI	CTCTTC	3 cut(s)	292, 376, 1697
EclXI	CGGCCG	1 cut(s)	271
Eco147I	AGGCCT	2 cut(s)	1108, 1891
Eco31I	GGTCTC	2 cut(s)	567, 1093
Eco47I	GGWCC	1 cut(s)	101
Eco52I	CGGCCG	1 cut(s)	271
Eco57I	CTGAAG	1 cut(s)	1512
EcoRI	GAATTC	2 cut(s)	542, 1664
EcoRII	CCWGG	2 cut(s)	478, 1757
EcoT22I	ATGCAT	1 cut(s)	1770
FaeI	CATG	7 cut(s)	133, 499, 593, 811, 924, 1772, 1927
FalI	AAGNNNNNCTT	6 cut(s)	736, 768, 749, 781, 882, 914
FaqI	GGGAC	3 cut(s)	437, 622, 1754
FatI	CATG	7 cut(s)	129, 495, 589, 807, 920, 1768, 1923
FbaI	TGATCA	1 cut(s)	1818
Fnu4HI	GCNGC	2 cut(s)	33, 36
FokI	GGATG	4 cut(s)	470, 727, 1245, 1374
Fsp4HI	GCNGC	2 cut(s)	33, 36
GlaI	GCGC	2 cut(s)	1527, 1651
GluI	GCNGC	2 cut(s)	33, 36
GsuI	CTGGAG	1 cut(s)	1780
HaeII	RGCGCY	1 cut(s)	1653
HaeIII	GGCC	6 cut(s)	44, 273, 1108, 1671, 1891, 1933
HapII	CCGG	6 cut(s)	270, 455, 771, 969, 1085, 1573
HhaI	GCGC	2 cut(s)	1528, 1652
Hin1II	CATG	7 cut(s)	133, 499, 593, 811, 924, 1772, 1927
Hin6I	GCGC	2 cut(s)	1526, 1650
HinP1I	GCGC	2 cut(s)	1526, 1650
HindIII	AAGCTT	4 cut(s)	200, 891, 1126, 1220
HpaII	CCGG	6 cut(s)	270, 455, 771, 969, 1085, 1573
HphI	GGTGA	5 cut(s)	780, 1651, 1736, 1747, 1790
Hpy166II	GTNNAC	4 cut(s)	434, 465, 676, 1458
Hpy188III	TCNNGA	9 cut(s)	169, 383, 563, 771, 779, 979, 1370, 1559, 1620
Hpy8I	GTNNAC	4 cut(s)	434, 465, 676, 1458
Hpy99I	CGWCG	3 cut(s)	239, 668, 743
HpyAV	CCTTC	5 cut(s)	355, 451, 762, 1300, 1617
HpyCH4III	ACNGT	3 cut(s)	469, 668, 792
HpyCH4IV	ACGT	2 cut(s)	553, 974
HpyCH4V	TGCA	6 cut(s)	1055, 1510, 1768, 1814, 1866, 1923
HpyF10VI	GCNNNNNNNGC	8 cut(s)	41, 203, 275, 491, 496, 699, 998, 1258
HpyF3I	CTNAG	9 cut(s)	120, 187, 257, 960, 1022, 1263, 1345, 1499, 1941
HpySE526I	ACGT	2 cut(s)	553, 974
Hsp92II	CATG	7 cut(s)	133, 499, 593, 811, 924, 1772, 1927
HspAI	GCGC	2 cut(s)	1526, 1650
Kpn2I	TCCGGA	1 cut(s)	770
KroI	GCCGGC	1 cut(s)	269
KroNI	GCCGGC	1 cut(s)	271
Ksp22I	TGATCA	1 cut(s)	1818
Kzo9I	GATC	8 cut(s)	253, 687, 731, 773, 981, 1193, 1818, 1828
LmnI	GCTCC	3 cut(s)	1610, 1900, 2023
Lsp1109I	GCAGC	2 cut(s)	19, 22
LweI	GCATC	1 cut(s)	496
MabI	ACCWGGT	1 cut(s)	478
MaeII	ACGT	2 cut(s)	553, 974
MaeIII	GTNAC	6 cut(s)	80, 115, 247, 746, 940, 1502
MalI	GATC	8 cut(s)	255, 689, 733, 775, 983, 1195, 1820, 1830
MboI	GATC	8 cut(s)	253, 687, 731, 773, 981, 1193, 1818, 1828
MfeI	CAATTG	1 cut(s)	1520
MflI	RGATCY	1 cut(s)	773
MhlI	GDGCHC	1 cut(s)	2020
MlyI	GAGTC	6 cut(s)	248, 294, 1453, 1625, 1651, 1948
MmeI	TCCRAC	5 cut(s)	322, 692, 796, 1266, 1689
Mph1103I	ATGCAT	1 cut(s)	1770
MroI	TCCGGA	1 cut(s)	770
MroNI	GCCGGC	1 cut(s)	269
MseI	TTAA	1 cut(s)	111
MspA1I	CMGCKG	1 cut(s)	99
MspI	CCGG	6 cut(s)	270, 455, 771, 969, 1085, 1573
MspR9I	CCNGG	5 cut(s)	455, 480, 969, 1574, 1759
MunI	CAATTG	1 cut(s)	1520
Mva1269I	GAATGC	2 cut(s)	1770, 1879
MvaI	CCWGG	2 cut(s)	480, 1759
MwoI	GCNNNNNNNGC	8 cut(s)	41, 203, 275, 491, 496, 699, 998, 1258
NaeI	GCCGGC	1 cut(s)	271
NciI	CCSGG	3 cut(s)	455, 969, 1574
NdeII	GATC	8 cut(s)	253, 687, 731, 773, 981, 1193, 1818, 1828
NgoMIV	GCCGGC	1 cut(s)	269
NlaIII	CATG	7 cut(s)	133, 499, 593, 811, 924, 1772, 1927
NmeAIII	GCCGAG	1 cut(s)	299
NmuCI	GTSAC	3 cut(s)	80, 115, 247
NsiI	ATGCAT	1 cut(s)	1770
NspI	RCATGY	2 cut(s)	811, 924
NspV	TTCGAA	2 cut(s)	336, 1226
PceI	AGGCCT	2 cut(s)	1108, 1891
PciI	ACATGT	2 cut(s)	807, 920
PctI	GAATGC	2 cut(s)	1770, 1879
PdiI	GCCGGC	1 cut(s)	271
PfeI	GAWTC	5 cut(s)	71, 165, 390, 559, 1631
PflFI	GACNNNGTC	1 cut(s)	1159
PflMI	CCANNNNNTGG	1 cut(s)	569
PfoI	TCCNGGA	1 cut(s)	453
PkrI	GCNGC	2 cut(s)	34, 37
PleI	GAGTC	6 cut(s)	247, 294, 1453, 1624, 1650, 1948
PpsI	GAGTC	6 cut(s)	247, 294, 1453, 1624, 1650, 1948
PscI	ACATGT	2 cut(s)	807, 920
Psp6I	CCWGG	2 cut(s)	478, 1757
PspGI	CCWGG	2 cut(s)	478, 1757
PspPI	GGNCC	1 cut(s)	101
PstNI	CAGNNNCTG	1 cut(s)	1270
PsuI	RGATCY	1 cut(s)	773
PsyI	GACNNNGTC	1 cut(s)	1159
RsaI	GTAC	2 cut(s)	806, 2002
RsaNI	GTAC	2 cut(s)	805, 2001
SaqAI	TTAA	1 cut(s)	111
SatI	GCNGC	2 cut(s)	33, 36
Sau3AI	GATC	8 cut(s)	253, 687, 731, 773, 981, 1193, 1818, 1828
Sau96I	GGNCC	1 cut(s)	101
SchI	GAGTC	6 cut(s)	248, 294, 1453, 1625, 1651, 1948
ScrFI	CCNGG	5 cut(s)	455, 480, 969, 1574, 1759
SduI	GDGCHC	1 cut(s)	2020
SexAI	ACCWGGT	1 cut(s)	478
SfaNI	GCATC	1 cut(s)	496
SfcI	CTRYAG	1 cut(s)	1008
SfuI	TTCGAA	2 cut(s)	336, 1226
SinI	GGWCC	1 cut(s)	101
SseBI	AGGCCT	2 cut(s)	1108, 1891
SsiI	CCGC	3 cut(s)	99, 408, 1252
StuI	AGGCCT	2 cut(s)	1108, 1891
StyD4I	CCNGG	5 cut(s)	453, 478, 967, 1572, 1757
TaaI	ACNGT	3 cut(s)	469, 668, 792
TaiI	ACGT	2 cut(s)	556, 977
TaqI	TCGA	7 cut(s)	336, 540, 546, 1226, 1619, 1634, 1957
TaqII	GACCGA	1 cut(s)	1117
TatI	WGTACW	2 cut(s)	804, 2000
TfiI	GAWTC	5 cut(s)	71, 165, 390, 559, 1631
Tru1I	TTAA	1 cut(s)	111
Tru9I	TTAA	1 cut(s)	111
TscAI	CASTG	7 cut(s)	199, 223, 256, 436, 1251, 1821, 1875
TseFI	GTSAC	3 cut(s)	80, 115, 247
TseI	GCWGC	2 cut(s)	32, 35
Tsp45I	GTSAC	3 cut(s)	80, 115, 247
TspDTI	ATGAA	4 cut(s)	363, 1137, 1319, 1785
TspGWI	ACGGA	3 cut(s)	756, 1245, 1443
TspRI	CASTG	7 cut(s)	199, 223, 256, 436, 1251, 1821, 1875
Tth111I	GACNNNGTC	1 cut(s)	1159
Van91I	CCANNNNNTGG	1 cut(s)	569
VpaK11BI	GGWCC	1 cut(s)	101
XapI	RAATTY	4 cut(s)	542, 720, 1057, 1664
XceI	RCATGY	2 cut(s)	811, 924
Zsp2I	ATGCAT	1 cut(s)	1770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.