RchiOBHm_Chr1g0334661

Lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012656
Physical Location & Seq
Forward (+)
26748568 .. 26750137
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56335

Sequence Viewer

Length: 1497 bp
ATGAGTTGCTTCAACTGGTTGCTCTATTTAGCTGCTGCTTTGGCCGTGATACGCAACGAGCTTCTAGTTGATTCAGCTAGTGACAAGAAGCACCACAGCGGACCAGTTGTTAATGTGACCAAGCACCTCATGTTCCCAGATTTCAACCTCTCCTACAACCCCCGAATCTTGAATGATGTAAAGCTCTTAGGCAGTGCCAAGCTTGCTTCAAAAACCAGTGCTATACAAATCCCCGACGAGTCTCATGTCACTGATCTCAGACACCAAGCCGGCCGAGCCATCTACTCTTCTTCAATCCGACTCCTGGACCCTCACACTGAAACCCCTGCCTCTTTTGAAACAACCTTCTCTTTTCAATTCCACAATGCCTCTTCATCTTCTCCTGAACGAATCCAAACTACTCAAAGCGGTGGTTATGGTAATGGCGGCAGTGGACTAGCCTTCGTCATCGTCCCGGATGAGTTCACAGTTGGCAGACCTGGTGCTTGGCTTGCCATGCTCAACGATGCTTGTGAAGATGACTACAAGGTTATAACAATCGAATTCGACACACGTTTGAATCCAGAGTTTGGAGACCCTAATGATAACCATGTAGGTATCAACTTGGGGACTATTATCTCCACTGAAACAATCAACGCTTCTGATGTTGGCGTCTTCCTCAACGACGGTTCAATCCACCGAGCTTGGATAAGCTACAACGGCTCACGCCGTTGGATGGAAATTCGTTTGGGATCAGACGACGGAAGTTACCCTGCAAAGCCAGTCTTCTCCGGATCTCTTGACCTCTCACCGTTTCTGAATGAGTACATGTTCGTTGGATTTTCGGCTTCCACAGGCAATCACACCCAAATCCACAGCGTGCTTTCTTGGAACTTCACTTCTGTTAGTCAAGCTTTTCTTCGTGTGCCGTCAATAGAGACATGTGAGAGCAAGATTGTTGTTACTGAAAACTCTCGTGCTGAGCCTCCCGGCACGTTCTTGATCTTTATAGCTGTGCTGGCACTTGCCATAGCTGTTATGCTTAGCCTTTACTATAACAGCAAGCGTAGAAGTGCAAATTCGGATAGTGCTAGTATAGTGTTGCCGGAGAAGAAGCAGAGACCGAGACCTCCAAACAAGCCTAGAAGCTTCACAATCTCGGAGATTTCATTGGCGACTAGGTCTTTCGGAGCGCTAGAGATACTTGGAGGTGGATCAAAAGGGGTGTGCTACCGAGGGAAGCTTCCGAACGGATGTCAAGTGGCAGTGAAGAGGAGATTGATGAAGGAAATCAAGGGTATTAGCAGGGTTAGACACCCGAACTTAGTTTCCATTAGAGGATGGTGTCAGGACAAGAAAGAGATTATGGTTGTGTATGAGTTTTTCTCCAATGGAAGCCTTGATAAGTGGCTGTTTGGTGTTGGTGTTCTTCCGTGGACTCGGAGGTTGATGCAGAACAGATGGCAGTCCAATTTGAAGAACAATTGGATCGTGCCAAAGGTGGAAAACGAAAAGTGA

Protein Analysis

498

Amino Acids

55.28

Weight (kDa)

8.97

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 58 - 296 1.1e-50 Legume lectin domain
Lectin_L-type_dom PF18483 64 - 286 3.6e-09 Legume lectin beta-barrel domain
Pkinase PF00069 392 - 464 1.7e-06 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 411 - 464 1.8e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g33120
malus_domestica MD01G1230400.v1.1
prunus_persica Prupe.2G321100_v2.0.a1
pyrus_communis pycom01g23840
rosa_chinensis RchiOBHm_Chr1g0334661 RchiOBHm_Chr1g0383191
rosa_laevigata RLG00000026124
rosa_multiflora Rmu_sc0008563.1_g000003
rosa_roxburghii Rroxscaffold_4G00277520
rosa_rugosa Rorug01G0126300 Rorug01G0439400
rosa_samantha Rh1AG139900 Rh1AG465000 Rh1BG422400 Rh1CG131800 Rh1CG435600 Rh1DG143800 Rh1DG455100
rosa_wichuraiana Rw1G040750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
AccB7I CCANNNNNTGG 1 cut(s) 569
AccIII TCCGGA 1 cut(s) 770
AciI CCGC 3 cut(s) 99, 408, 426
AclWI GGATC 4 cut(s) 739, 781, 1201, 1475
AcoI YGGCCR 2 cut(s) 42, 271
AcsI RAATTY 3 cut(s) 542, 720, 1057
AcyI GRCGYC 1 cut(s) 651
AdeI CACNNNGTG 1 cut(s) 859
AfaI GTAC 1 cut(s) 806
AfeI AGCGCT 1 cut(s) 1173
AfiI CCNNNNNNNGG 3 cut(s) 304, 569, 715
AflIII ACRYGT 3 cut(s) 551, 807, 920
AjnI CCWGG 2 cut(s) 303, 478
AloI GAACNNNNNNTCC 2 cut(s) 1292, 1324
Alw26I GTCTC 5 cut(s) 246, 567, 911, 1093, 1099
AlwI GGATC 4 cut(s) 739, 781, 1201, 1475
Aor13HI TCCGGA 1 cut(s) 770
Aor51HI AGCGCT 1 cut(s) 1173
AoxI GGCC 2 cut(s) 42, 271
ApeKI GCWGC 2 cut(s) 32, 35
ApoI RAATTY 3 cut(s) 542, 720, 1057
AspLEI GCGC 1 cut(s) 1174
AspS9I GGNCC 2 cut(s) 101, 307
AsuC2I CCSGG 2 cut(s) 455, 969
AsuHPI GGTGA 1 cut(s) 780
AvaII GGWCC 2 cut(s) 101, 307
BauI CACGAG 1 cut(s) 954
BbsI GAAGAC 2 cut(s) 646, 757
BbvI GCAGC 2 cut(s) 19, 22
BccI CCATC 4 cut(s) 287, 709, 1314, 1434
BceAI ACGGC 4 cut(s) 29, 693, 715, 892
BciT130I CCWGG 2 cut(s) 305, 480
BcnI CCSGG 2 cut(s) 455, 969
BcoDI GTCTC 5 cut(s) 246, 567, 911, 1093, 1099
BfaI CTAG 7 cut(s) 65, 78, 437, 1071, 1122, 1158, 1175
BfoI RGCGCY 1 cut(s) 1175
BisI GCNGC 3 cut(s) 33, 36, 427
BlpI GCTNAGC 2 cut(s) 960, 1022
BlsI GCNGC 3 cut(s) 34, 37, 428
Bme1390I CCNGG 4 cut(s) 305, 455, 480, 969
Bme18I GGWCC 2 cut(s) 101, 307
BmgT120I GGNCC 2 cut(s) 101, 307
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 4 cut(s) 305, 455, 480, 969
BmsI GCATC 2 cut(s) 496, 1419
BpiI GAAGAC 2 cut(s) 646, 757
BplI GAGNNNNNCTC 2 cut(s) 1349, 1381
Bpu1102I GCTNAGC 2 cut(s) 960, 1022
BpuMI CCSGG 2 cut(s) 455, 969
BsaHI GRCGYC 1 cut(s) 651
BsaI GGTCTC 3 cut(s) 567, 1093, 1099
BsaJI CCNNGG 2 cut(s) 1213, 1412
BsaWI WCCGGW 1 cut(s) 770
Bsc4I CCNNNNNNNGG 3 cut(s) 304, 569, 715
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 4 cut(s) 20, 104, 216, 761
BseAI TCCGGA 1 cut(s) 770
BseBI CCWGG 2 cut(s) 305, 480
BseDI CCNNGG 2 cut(s) 1213, 1412
BseGI GGATG 4 cut(s) 463, 720, 1238, 1325
BseLI CCNNNNNNNGG 3 cut(s) 304, 569, 715
BseMII CTCAG 2 cut(s) 271, 951
BseNI ACTGG 4 cut(s) 20, 104, 216, 761
BseRI GAGGAG 1 cut(s) 1267
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 2 cut(s) 19, 22
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 2 cut(s) 44, 273
BsiEI CGRYCG 1 cut(s) 274
BsiSI CCGG 5 cut(s) 270, 455, 771, 969, 1085
BslFI GGGAC 2 cut(s) 437, 622
BslI CCNNNNNNNGG 3 cut(s) 304, 569, 715
BsmAI GTCTC 5 cut(s) 246, 567, 911, 1093, 1099
BsmFI GGGAC 2 cut(s) 437, 622
BsnI GGCC 2 cut(s) 44, 273
Bso31I GGTCTC 3 cut(s) 567, 1093, 1099
Bsp13I TCCGGA 1 cut(s) 770
Bsp143I GATC 6 cut(s) 253, 731, 773, 981, 1193, 1467
Bsp1720I GCTNAGC 2 cut(s) 960, 1022
BspACI CCGC 3 cut(s) 99, 408, 426
BspANI GGCC 2 cut(s) 44, 273
BspCNI CTCAG 2 cut(s) 270, 952
BspEI TCCGGA 1 cut(s) 770
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 4 cut(s) 739, 781, 1201, 1475
BspTNI GGTCTC 3 cut(s) 567, 1093, 1099
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 4 cut(s) 20, 104, 216, 761
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 2 cut(s) 1213, 1412
BssMI GATC 6 cut(s) 253, 731, 773, 981, 1193, 1467
BssNI GRCGYC 1 cut(s) 651
BssSI CACGAG 1 cut(s) 954
Bst2BI CACGAG 1 cut(s) 954
Bst2UI CCWGG 2 cut(s) 305, 480
Bst4CI ACNGT 3 cut(s) 469, 668, 792
Bst6I CTCTTC 3 cut(s) 292, 376, 1244
BstACI GRCGYC 1 cut(s) 651
BstC8I GCNNGC 6 cut(s) 204, 271, 492, 860, 999, 1043
BstDEI CTNAG 5 cut(s) 187, 257, 960, 1022, 1303
BstDSI CCRYGG 1 cut(s) 1412
BstF5I GGATG 4 cut(s) 463, 720, 1238, 1325
BstH2I RGCGCY 1 cut(s) 1175
BstHHI GCGC 1 cut(s) 1174
BstKTI GATC 6 cut(s) 256, 734, 776, 984, 1196, 1470
BstMAI GTCTC 5 cut(s) 246, 567, 911, 1093, 1099
BstMBI GATC 6 cut(s) 253, 731, 773, 981, 1193, 1467
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 7 cut(s) 41, 203, 275, 491, 496, 699, 998
BstNI CCWGG 2 cut(s) 305, 480
BstNSI RCATGY 2 cut(s) 811, 924
BstSCI CCNGG 4 cut(s) 303, 453, 478, 967
BstV1I GCAGC 2 cut(s) 19, 22
BstV2I GAAGAC 2 cut(s) 646, 757
BstX2I RGATCY 1 cut(s) 773
BstYI RGATCY 1 cut(s) 773
BstZI CGGCCG 1 cut(s) 271
BsuRI GGCC 2 cut(s) 44, 273
BtgI CCRYGG 1 cut(s) 1412
BtsCI GGATG 4 cut(s) 463, 720, 1238, 1325
BtsI GCAGTG 3 cut(s) 199, 436, 1251
BtsIMutI CAGTG 7 cut(s) 199, 223, 249, 315, 436, 621, 1251
Cac8I GCNNGC 6 cut(s) 204, 271, 492, 860, 999, 1043
CfoI GCGC 1 cut(s) 1174
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 2 cut(s) 101, 307
CseI GACGC 1 cut(s) 640
CsiI ACCWGGT 1 cut(s) 478
Csp6I GTAC 1 cut(s) 805
CspCI CAANNNNNGTGG 2 cut(s) 665, 700
CviAII CATG 6 cut(s) 130, 245, 496, 590, 808, 921
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 5 cut(s) 187, 257, 960, 1022, 1303
DpnI GATC 6 cut(s) 255, 733, 775, 983, 1195, 1469
DpnII GATC 6 cut(s) 253, 731, 773, 981, 1193, 1467
DraIII CACNNNGTG 1 cut(s) 859
EaeI YGGCCR 2 cut(s) 42, 271
EagI CGGCCG 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 292, 376, 1244
EarI CTCTTC 3 cut(s) 292, 376, 1244
EclXI CGGCCG 1 cut(s) 271
Eco31I GGTCTC 3 cut(s) 567, 1093, 1099
Eco47I GGWCC 2 cut(s) 101, 307
Eco47III AGCGCT 1 cut(s) 1173
Eco52I CGGCCG 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 542
EcoRII CCWGG 2 cut(s) 303, 478
FaeI CATG 6 cut(s) 133, 248, 499, 593, 811, 924
FalI AAGNNNNNCTT 4 cut(s) 749, 781, 882, 914
FaqI GGGAC 2 cut(s) 437, 622
FatI CATG 6 cut(s) 129, 244, 495, 589, 807, 920
Fnu4HI GCNGC 3 cut(s) 33, 36, 427
FokI GGATG 4 cut(s) 470, 727, 1245, 1332
Fsp4HI GCNGC 3 cut(s) 33, 36, 427
FspBI CTAG 7 cut(s) 65, 78, 437, 1071, 1122, 1158, 1175
GlaI GCGC 1 cut(s) 1173
GluI GCNGC 3 cut(s) 33, 36, 427
HaeII RGCGCY 1 cut(s) 1175
HaeIII GGCC 2 cut(s) 44, 273
HapII CCGG 5 cut(s) 270, 455, 771, 969, 1085
HgaI GACGC 1 cut(s) 640
HhaI GCGC 1 cut(s) 1174
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 6 cut(s) 133, 248, 499, 593, 811, 924
Hin6I GCGC 1 cut(s) 1172
HinP1I GCGC 1 cut(s) 1172
HindIII AAGCTT 4 cut(s) 200, 891, 1126, 1220
HinfI GANTC 7 cut(s) 71, 165, 239, 300, 390, 559, 1417
HpaII CCGG 5 cut(s) 270, 455, 771, 969, 1085
HphI GGTGA 1 cut(s) 780
Hpy166II GTNNAC 3 cut(s) 434, 465, 1416
Hpy188III TCNNGA 7 cut(s) 169, 383, 563, 771, 779, 979, 1328
Hpy8I GTNNAC 3 cut(s) 434, 465, 1416
Hpy99I CGWCG 3 cut(s) 239, 668, 743
HpyAV CCTTC 3 cut(s) 355, 451, 1258
HpyCH4III ACNGT 3 cut(s) 469, 668, 792
HpyCH4IV ACGT 2 cut(s) 553, 974
HpyCH4V TGCA 3 cut(s) 755, 1055, 1432
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 203, 275, 491, 496, 699, 998
HpyF3I CTNAG 5 cut(s) 187, 257, 960, 1022, 1303
HpySE526I ACGT 2 cut(s) 553, 974
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 6 cut(s) 133, 248, 499, 593, 811, 924
HspAI GCGC 1 cut(s) 1172
Kpn2I TCCGGA 1 cut(s) 770
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
Kzo9I GATC 6 cut(s) 253, 731, 773, 981, 1193, 1467
LmnI GCTCC 1 cut(s) 1169
Lsp1109I GCAGC 2 cut(s) 19, 22
LweI GCATC 2 cut(s) 496, 1419
MabI ACCWGGT 1 cut(s) 478
MaeI CTAG 7 cut(s) 65, 78, 437, 1071, 1122, 1158, 1175
MaeII ACGT 2 cut(s) 553, 974
MaeIII GTNAC 5 cut(s) 80, 115, 247, 746, 940
MalI GATC 6 cut(s) 255, 733, 775, 983, 1195, 1469
MboI GATC 6 cut(s) 253, 731, 773, 981, 1193, 1467
MfeI CAATTG 1 cut(s) 1462
MflI RGATCY 1 cut(s) 773
MluCI AATT 6 cut(s) 356, 542, 720, 1057, 1450, 1462
MlyI GAGTC 3 cut(s) 248, 294, 1411
MmeI TCCRAC 3 cut(s) 322, 692, 796
MroI TCCGGA 1 cut(s) 770
MroNI GCCGGC 1 cut(s) 269
MseI TTAA 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 99
MspI CCGG 5 cut(s) 270, 455, 771, 969, 1085
MspR9I CCNGG 4 cut(s) 305, 455, 480, 969
MunI CAATTG 1 cut(s) 1462
MvaI CCWGG 2 cut(s) 305, 480
MwoI GCNNNNNNNGC 7 cut(s) 41, 203, 275, 491, 496, 699, 998
NaeI GCCGGC 1 cut(s) 271
NciI CCSGG 2 cut(s) 455, 969
NdeII GATC 6 cut(s) 253, 731, 773, 981, 1193, 1467
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 6 cut(s) 133, 248, 499, 593, 811, 924
NlaIV GGNNCC 1 cut(s) 309
NmeAIII GCCGAG 1 cut(s) 299
NmuCI GTSAC 3 cut(s) 80, 115, 247
NspI RCATGY 2 cut(s) 811, 924
PciI ACATGT 2 cut(s) 807, 920
PdiI GCCGGC 1 cut(s) 271
PfeI GAWTC 4 cut(s) 71, 165, 390, 559
PflFI GACNNNGTC 1 cut(s) 1159
PflMI CCANNNNNTGG 1 cut(s) 569
PfoI TCCNGGA 2 cut(s) 303, 453
PkrI GCNGC 3 cut(s) 34, 37, 428
PleI GAGTC 3 cut(s) 247, 294, 1411
PpsI GAGTC 3 cut(s) 247, 294, 1411
PscI ACATGT 2 cut(s) 807, 920
PsiI TTATAA 1 cut(s) 533
Psp6I CCWGG 2 cut(s) 303, 478
PspGI CCWGG 2 cut(s) 303, 478
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 2 cut(s) 101, 307
PsuI RGATCY 1 cut(s) 773
PsyI GACNNNGTC 1 cut(s) 1159
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 3 cut(s) 33, 36, 427
Sau3AI GATC 6 cut(s) 253, 731, 773, 981, 1193, 1467
Sau96I GGNCC 2 cut(s) 101, 307
SchI GAGTC 3 cut(s) 248, 294, 1411
ScrFI CCNGG 4 cut(s) 305, 455, 480, 969
SexAI ACCWGGT 1 cut(s) 478
SfaNI GCATC 2 cut(s) 496, 1419
SinI GGWCC 2 cut(s) 101, 307
Sse9I AATT 6 cut(s) 356, 542, 720, 1057, 1450, 1462
SsiI CCGC 3 cut(s) 99, 408, 426
SspMI CTAG 7 cut(s) 65, 78, 437, 1071, 1122, 1158, 1175
StyD4I CCNGG 4 cut(s) 303, 453, 478, 967
TaaI ACNGT 3 cut(s) 469, 668, 792
TaiI ACGT 2 cut(s) 556, 977
TaqI TCGA 2 cut(s) 540, 546
TaqII GACCGA 1 cut(s) 1117
TasI AATT 6 cut(s) 356, 542, 720, 1057, 1450, 1462
TatI WGTACW 1 cut(s) 804
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 4 cut(s) 71, 165, 390, 559
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 7 cut(s) 199, 223, 256, 322, 436, 628, 1251
TseFI GTSAC 3 cut(s) 80, 115, 247
TseI GCWGC 2 cut(s) 32, 35
Tsp45I GTSAC 3 cut(s) 80, 115, 247
TspDTI ATGAA 3 cut(s) 363, 1137, 1277
TspGWI ACGGA 3 cut(s) 756, 1245, 1401
TspRI CASTG 7 cut(s) 199, 223, 256, 322, 436, 628, 1251
Tth111I GACNNNGTC 1 cut(s) 1159
Van91I CCANNNNNTGG 1 cut(s) 569
VpaK11BI GGWCC 2 cut(s) 101, 307
XapI RAATTY 3 cut(s) 542, 720, 1057
XceI RCATGY 2 cut(s) 811, 924
XspI CTAG 7 cut(s) 65, 78, 437, 1071, 1122, 1158, 1175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.