Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0012656

Physical Location & Seq

Genomic Coordinates Forward (+)

21030740 .. 21033194

Gene Structure 2455 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g23840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGTCCTTACGTACTTATCACAATAAGTACCACTTCAAGCTTGTCCACCCAAATATTTCACTCACTTGTCTTCACTCTTTTAATAGAGACGTGCTCGACCGAAGATTCCAACTTCGTGAGACGATTTCCGGCGACGTTCTGCACGGAGGAAATTTGTTGGAATTCTTCTTCGCCGCCGCGGAAATGGGTTGCTGCACCTACAAGTACTACTTCTTGTGGTTGACATGCTCGGCTTATCTTGTTCTTGTTCAAGGTAGGGAGAAGAAGCACCATTTTAATGGACCTGTTGTTAACGTGACGAAGCATCTCAATTTCCAAGATTTTAACTTTGTCAAAAACCCCAGAGTTTTGGAAGATGTAAGGCTCCTAGGCAGTGCCAAAATCTCAAGCTCATCGTCAGCCATACAAATCCCCGATGATCATGATCAGTACTCCGACGTCAACGGCAGCGATGATATCCATCATCTAAGACAGCAAGCAGGCCGAGCCCTCTACTCCTCTCCCATCCGACTCCTCGACCCTCACACTCAATCGCCTGCTTCTTTTCAAACAACATTTTCTTTCCAATTCCACAACGGTTCCAATTTAAACGCTTCTGATCATGGTGGTAGTGGCCTCACCTTCATCATTGTCCCTGACGAGTTCACTGTCGGAAGGCCTGGCCCGTGGCTCGCCATGCTTAACGACGCTTGTGAAGATGATTACAAGGCGGTTGCAGTCGAGTTTGACACCCGTCTCAACCCTGAATTCGGAGACCCGAATGACAACCATGTTGGAATCAACTTGGGAACAATTGTCTCCACCGAAACAATTAACGCATCGGAAGTTGGTGTATTTCTCAACGACGGCTCAGTCCACCGAGCGTGGATTAGCTACGATGGTCCGAACCGTTGGATGGAAATTCGTTTGGGATCAGACGACAGTACTAATCACCCGCCACGAAAACCAATCTTCTCTGGCCCTCTTGATCTCTCACCATTTCTCAACGAGTACATGTTTGTCGGATTCTCTGCTTCAACAGGGAACCACACGCAAATCCACACCGTCCTCTCGTGGAATTTCACCTCCACTAGTCAAGCTTTTCTCTGGATGCCGTCGATCGAAGCGTGCGAGAGTAACATAATTGTGACTGGCAGAGGAAGTAGTGCCCCGAGCGCTGAGCCTCGCGGCACTTTCTTAATCTTCATTGCTGTGGTGGTTTTGGCTCTGGCTGTGGTGGCTAGTCTGTACTATAGCGGCAGGCGCAAAACCGCCGGAATATCAGACACTCCTGTTGTGTTGCCGGAGAAGAAGCAGAGACCGAGGCCTCCGAACAAGCCTCGATACTTCACAATCTGCGAAATTTCCTTGGCCACTCAGTCCTTCAGCGAGTACGAAATTGTTGGTAGCAGCGAAAGAGGCGTGTACTATAGAGGGAAGCTTTTGAATGGTTGTCAGGTGGCTGTGAAGCGGTTTTCTTCTCTGTTTTTGAGCTCGGAAGGGCTAGATAGGCGGAGACTTTTCAAGGAAATAAAGGGGATTAGCCGGGTTAGGCATCCGAATCTGGTTCCAATCCGAGGGTGGTGTCAAGACAAGAAGGAAACCATGGTTGTGTATGAGTTTTTCCCAAATGGAAGCCTTGATAAATGGTTGTTTGGGGTGGGGGTGCTTCCGTGGACGCGGCGGTTCAAGGTTGTGAAAGATGTGGCTGACGCCCTCAGTTTCTTGCACTCCAAACAATTATCCCACAAGAACTTGAAGACAACTAGTGTCTTTCTTGACGTCAGTTTCCGGGCTGTTTTAGGCGATTTCGGGTTTGTGCTAAATGGTGCAGAATCAAGAAGGTTTGAGTCCGCAGTGAGCCAAAGTGCGGATGTTTTCGAGTTTGGTCTGTTTGTGTTGGAAGTGGTCGGTGGGAGACGGCGATTGGAGGCGGATTCGGGGCCAGTAGAGGAGAGGGATCTGTTGGATTTTGCATGGAGAATGCATGAGATTGATGAGAGGGTGAGGGTAGTGGATAAGAGGATGGGCACTGTGATTAATTTGGAGCAAGCAATTAGGGTTATGCAAATCGGGTTGCTCTGCACTGTGAATGTGACCAAAGGGACACACAATTGCAATTGGCGCAAAAGCGAGAGCAGTGGCGTTCTAGGATTCATACAGCTAACCGTATTTAATGGGCTAAGACTTGGTTGTTGTTGTTGTTATTGTTATAATTGGTGGTCTATTAGTATGGATGTGAAAAGATGTGCTAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002239 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0016020 GO:0043207 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Lectin_legB Pkinase_Tyr

Protein Analysis

746

Amino Acids

83.9

Weight (kDa)

8.21

Isoelectric Point (pI)

39.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	104 - 359	2.9e-54	Legume lectin domain
Lectin_L-type_dom	PF18483	182 - 348	4.4e-09	Legume lectin beta-barrel domain
Pkinase	PF00069	456 - 611	1.3e-17	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	459 - 603	2.4e-18	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012656)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33120
malus_domestica	MD01G1230400.v1.1
prunus_persica	Prupe.2G321100_v2.0.a1
pyrus_communis	pycom01g23840
rosa_chinensis	RchiOBHm_Chr1g0334661 RchiOBHm_Chr1g0383191
rosa_laevigata	RLG00000026124
rosa_multiflora	Rmu_sc0008563.1_g000003
rosa_roxburghii	Rroxscaffold_4G00277520
rosa_rugosa	Rorug01G0126300 Rorug01G0439400
rosa_samantha	Rh1AG139900 Rh1AG465000 Rh1BG422400 Rh1CG131800 Rh1CG435600 Rh1DG143800 Rh1DG455100
rosa_wichuraiana	Rw1G040750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2193
AasI	GACNNNNNNGTC	1 cut(s)	851
AatII	GACGTC	2 cut(s)	441, 1764
AccII	CGCG	3 cut(s)	179, 1167, 1660
AclWI	GGATC	2 cut(s)	919, 1947
AcoI	YGGCCR	1 cut(s)	1350
AcsI	RAATTY	6 cut(s)	151, 161, 746, 900, 1057, 1341
AcuI	CTGAAG	1 cut(s)	1348
AcyI	GRCGYC	3 cut(s)	438, 1692, 1761
AfaI	GTAC	9 cut(s)	13, 29, 206, 431, 925, 992, 1229, 1373, 1406
AfeI	AGCGCT	1 cut(s)	1156
AfiI	CCNNNNNNNGG	4 cut(s)	749, 895, 1556, 1849
AflIII	ACRYGT	1 cut(s)	993
AgsI	TTSAA	8 cut(s)	37, 251, 548, 1017, 1426, 1504, 1669, 1738
AhlI	ACTAGT	2 cut(s)	1070, 1745
AjiI	CACGTC	1 cut(s)	91
AjnI	CCWGG	1 cut(s)	658
AluBI	AGCT	7 cut(s)	40, 390, 873, 1079, 1420, 1473, 2143
AluI	AGCT	7 cut(s)	40, 390, 873, 1079, 1420, 1473, 2143
Alw21I	GWGCWC	2 cut(s)	96, 1475
Alw26I	GTCTC	8 cut(s)	81, 113, 740, 747, 802, 1291, 1489, 1891
AlwI	GGATC	2 cut(s)	919, 1947
Ama87I	CYCGRG	1 cut(s)	1150
Aor51HI	AGCGCT	1 cut(s)	1156
AoxI	GGCC	8 cut(s)	481, 613, 656, 661, 958, 1304, 1350, 1922
ApeKI	GCWGC	3 cut(s)	192, 447, 1389
ApoI	RAATTY	6 cut(s)	151, 161, 746, 900, 1057, 1341
AseI	ATTAAT	1 cut(s)	2019
AspA2I	CCTAGG	1 cut(s)	367
AspLEI	GCGC	3 cut(s)	1157, 1245, 2106
AspS9I	GGNCC	5 cut(s)	281, 662, 881, 959, 1922
AsuC2I	CCSGG	2 cut(s)	1526, 1772
AsuHPI	GGTGA	5 cut(s)	610, 923, 966, 1054, 1996
AvaI	CYCGRG	1 cut(s)	1150
AvaII	GGWCC	2 cut(s)	281, 881
AvrII	CCTAGG	1 cut(s)	367
BaeGI	GKGCMC	2 cut(s)	1150, 2012
BalI	TGGCCA	1 cut(s)	1352
BanII	GRGCYC	2 cut(s)	490, 1475
BauI	CACGAG	1 cut(s)	1051
BbsI	GAAGAC	2 cut(s)	62, 1745
Bbv12I	GWGCWC	2 cut(s)	96, 1475
BbvI	GCAGC	3 cut(s)	179, 459, 1401
BccI	CCATC	5 cut(s)	468, 512, 872, 889, 1999
BceAI	ACGGC	4 cut(s)	460, 862, 1078, 1916
BciT130I	CCWGG	1 cut(s)	660
BclI	TGATCA	3 cut(s)	418, 424, 598
BcnI	CCSGG	2 cut(s)	1526, 1772
BcoDI	GTCTC	8 cut(s)	81, 113, 740, 747, 802, 1291, 1489, 1891
BcuI	ACTAGT	2 cut(s)	1070, 1745
BfaI	CTAG	6 cut(s)	368, 1071, 1221, 1484, 1746, 2129
BfmI	CTRYAG	2 cut(s)	1231, 1408
BfoI	RGCGCY	1 cut(s)	1158
BisI	GCNGC	8 cut(s)	174, 177, 193, 448, 1168, 1237, 1390, 1661
BlnI	CCTAGG	1 cut(s)	367
BlpI	GCTNAGC	1 cut(s)	1158
BlsI	GCNGC	8 cut(s)	175, 178, 194, 449, 1169, 1238, 1391, 1662
BmcAI	AGTACT	3 cut(s)	206, 431, 925
Bme1390I	CCNGG	3 cut(s)	660, 1526, 1772
Bme18I	GGWCC	2 cut(s)	281, 881
BmeT110I	CYCGRG	1 cut(s)	1150
BmgBI	CACGTC	1 cut(s)	91
BmgT120I	GGNCC	5 cut(s)	281, 662, 881, 959, 1922
BmiI	GGNNCC	5 cut(s)	365, 580, 1025, 1548, 1923
BmrFI	CCNGG	3 cut(s)	660, 1526, 1772
BmsI	GCATC	4 cut(s)	313, 827, 1080, 1543
BoxI	GACNNNNGTC	1 cut(s)	732
BpiI	GAAGAC	2 cut(s)	62, 1745
BplI	GAGNNNNNCTC	2 cut(s)	78, 110
Bpu1102I	GCTNAGC	1 cut(s)	1158
BpuEI	CTTGAG	1 cut(s)	370
BpuMI	CCSGG	2 cut(s)	1526, 1772
BsaAI	YACGTR	1 cut(s)	11
BsaBI	GATNNNNATC	2 cut(s)	423, 459
BsaHI	GRCGYC	3 cut(s)	438, 1692, 1761
BsaI	GGTCTC	2 cut(s)	747, 1291
BsaJI	CCNNGG	8 cut(s)	177, 367, 665, 1301, 1347, 1555, 1584, 1652
Bsc4I	CCNNNNNNNGG	4 cut(s)	749, 895, 1556, 1849
Bse1I	ACTGG	2 cut(s)	1135, 1925
Bse3DI	GCAATG	1 cut(s)	1185
Bse8I	GATNNNNATC	2 cut(s)	423, 459
BseBI	CCWGG	1 cut(s)	660
BseDI	CCNNGG	8 cut(s)	177, 367, 665, 1301, 1347, 1555, 1584, 1652
BseGI	GGATG	7 cut(s)	504, 900, 1095, 1534, 1858, 2010, 2221
BseJI	GATNNNNATC	2 cut(s)	423, 459
BseLI	CCNNNNNNNGG	4 cut(s)	749, 895, 1556, 1849
BseMI	GCAATG	1 cut(s)	1185
BseMII	CTCAG	4 cut(s)	864, 1149, 1370, 1711
BseNI	ACTGG	2 cut(s)	1135, 1925
BseRI	GAGGAG	3 cut(s)	487, 503, 1946
BseSI	GKGCMC	2 cut(s)	1150, 2012
BseXI	GCAGC	3 cut(s)	179, 459, 1401
BsgI	GTGCAG	4 cut(s)	125, 178, 1830, 2047
Bsh1236I	CGCG	3 cut(s)	179, 1167, 1660
Bsh1285I	CGRYCG	2 cut(s)	100, 1101
BshFI	GGCC	8 cut(s)	483, 615, 658, 663, 960, 1306, 1352, 1924
BsiEI	CGRYCG	2 cut(s)	100, 1101
BsiHKAI	GWGCWC	2 cut(s)	96, 1475
BsiHKCI	CYCGRG	1 cut(s)	1150
BsiSI	CCGG	5 cut(s)	129, 1254, 1283, 1525, 1771
BslFI	GGGAC	2 cut(s)	617, 2098
BslI	CCNNNNNNNGG	4 cut(s)	749, 895, 1556, 1849
BsmAI	GTCTC	8 cut(s)	81, 113, 740, 747, 802, 1291, 1489, 1891
BsmBI	CGTCTC	4 cut(s)	81, 113, 740, 1891
BsmFI	GGGAC	2 cut(s)	617, 2098
BsmI	GAATGC	1 cut(s)	1968
BsnI	GGCC	8 cut(s)	483, 615, 658, 663, 960, 1306, 1352, 1924
Bso31I	GGTCTC	2 cut(s)	747, 1291
BsoBI	CYCGRG	1 cut(s)	1150
Bsp1286I	GDGCHC	5 cut(s)	96, 490, 1150, 1475, 2012
Bsp143I	GATC	7 cut(s)	418, 424, 598, 911, 967, 1098, 1939
Bsp1720I	GCTNAGC	1 cut(s)	1158
Bsp19I	CCATGG	1 cut(s)	1584
BspANI	GGCC	8 cut(s)	483, 615, 658, 663, 960, 1306, 1352, 1924
BspCNI	CTCAG	4 cut(s)	863, 1150, 1369, 1710
BspFNI	CGCG	3 cut(s)	179, 1167, 1660
BspHI	TCATGA	1 cut(s)	421
BspLI	GGNNCC	5 cut(s)	365, 580, 1025, 1548, 1923
BspPI	GGATC	2 cut(s)	919, 1947
BspTNI	GGTCTC	2 cut(s)	747, 1291
BsrDI	GCAATG	1 cut(s)	1185
BsrI	ACTGG	2 cut(s)	1135, 1925
BssECI	CCNNGG	8 cut(s)	177, 367, 665, 1301, 1347, 1555, 1584, 1652
BssMI	GATC	7 cut(s)	418, 424, 598, 911, 967, 1098, 1939
BssNI	GRCGYC	3 cut(s)	438, 1692, 1761
BssSI	CACGAG	1 cut(s)	1051
BssT1I	CCWWGG	3 cut(s)	367, 1347, 1584
Bst2BI	CACGAG	1 cut(s)	1051
Bst2UI	CCWGG	1 cut(s)	660
Bst4CI	ACNGT	8 cut(s)	578, 649, 890, 923, 1045, 2014, 2068, 2149
BstACI	GRCGYC	3 cut(s)	438, 1692, 1761
BstBAI	YACGTR	1 cut(s)	11
BstC8I	GCNNGC	7 cut(s)	477, 481, 537, 672, 1108, 1241, 2031
BstDEI	CTNAG	6 cut(s)	467, 850, 1158, 1356, 1697, 2162
BstDSI	CCRYGG	4 cut(s)	177, 665, 1584, 1652
BstF5I	GGATG	7 cut(s)	504, 900, 1095, 1534, 1858, 2010, 2221
BstFNI	CGCG	3 cut(s)	179, 1167, 1660
BstH2I	RGCGCY	1 cut(s)	1158
BstHHI	GCGC	3 cut(s)	1157, 1245, 2106
BstKTI	GATC	7 cut(s)	421, 427, 601, 914, 970, 1101, 1942
BstMAI	GTCTC	8 cut(s)	81, 113, 740, 747, 802, 1291, 1489, 1891
BstMBI	GATC	7 cut(s)	418, 424, 598, 911, 967, 1098, 1939
BstMCI	CGRYCG	2 cut(s)	100, 1101
BstMWI	GCNNNNNNNGC	8 cut(s)	485, 676, 1154, 1217, 1242, 1398, 1489, 2103
BstNI	CCWGG	1 cut(s)	660
BstNSI	RCATGY	2 cut(s)	228, 997
BstPAI	GACNNNNGTC	1 cut(s)	732
BstSCI	CCNGG	3 cut(s)	658, 1524, 1770
BstSFI	CTRYAG	2 cut(s)	1231, 1408
BstSLI	GKGCMC	2 cut(s)	1150, 2012
BstSNI	TACGTA	1 cut(s)	11
BstUI	CGCG	3 cut(s)	179, 1167, 1660
BstV1I	GCAGC	3 cut(s)	179, 459, 1401
BstV2I	GAAGAC	2 cut(s)	62, 1745
BstX2I	RGATCY	1 cut(s)	1939
BstXI	CCANNNNNNTGG	2 cut(s)	278, 349
BstYI	RGATCY	1 cut(s)	1939
BsuRI	GGCC	8 cut(s)	483, 615, 658, 663, 960, 1306, 1352, 1924
BtgI	CCRYGG	4 cut(s)	177, 665, 1584, 1652
BtgZI	GCGATG	1 cut(s)	465
BtrI	CACGTC	1 cut(s)	91
BtsCI	GGATG	7 cut(s)	504, 900, 1095, 1534, 1858, 2010, 2221
BtsI	GCAGTG	3 cut(s)	379, 1842, 2125
BtsIMutI	CAGTG	6 cut(s)	379, 645, 1842, 2010, 2064, 2125
Cac8I	GCNNGC	7 cut(s)	477, 481, 537, 672, 1108, 1241, 2031
CciI	TCATGA	1 cut(s)	421
CfoI	GCGC	3 cut(s)	1157, 1245, 2106
Cfr13I	GGNCC	5 cut(s)	281, 662, 881, 959, 1922
Cfr42I	CCGCGG	1 cut(s)	180
CseI	GACGC	3 cut(s)	695, 1666, 1700
Csp6I	GTAC	9 cut(s)	12, 28, 205, 430, 924, 991, 1228, 1372, 1405
CviAII	CATG	9 cut(s)	225, 422, 602, 676, 770, 994, 1585, 1956, 1967
CviQI	GTAC	9 cut(s)	12, 28, 205, 430, 924, 991, 1228, 1372, 1405
DdeI	CTNAG	6 cut(s)	467, 850, 1158, 1356, 1697, 2162
DpnI	GATC	7 cut(s)	420, 426, 600, 913, 969, 1100, 1941
DpnII	GATC	7 cut(s)	418, 424, 598, 911, 967, 1098, 1939
DraI	TTTAAA	1 cut(s)	588
DrdI	GACNNNNNNGTC	1 cut(s)	851
DseDI	GACNNNNNNGTC	1 cut(s)	851
EaeI	YGGCCR	1 cut(s)	1350
EciI	GGCGGA	2 cut(s)	1507, 1928
Ecl136II	GAGCTC	1 cut(s)	1473
Eco105I	TACGTA	1 cut(s)	11
Eco130I	CCWWGG	3 cut(s)	367, 1347, 1584
Eco147I	AGGCCT	2 cut(s)	658, 1306
Eco24I	GRGCYC	2 cut(s)	490, 1475
Eco31I	GGTCTC	2 cut(s)	747, 1291
Eco32I	GATATC	1 cut(s)	457
Eco47I	GGWCC	2 cut(s)	281, 881
Eco47III	AGCGCT	1 cut(s)	1156
Eco53kI	GAGCTC	1 cut(s)	1473
Eco57I	CTGAAG	1 cut(s)	1348
Eco88I	CYCGRG	1 cut(s)	1150
EcoICRI	GAGCTC	1 cut(s)	1473
EcoRI	GAATTC	2 cut(s)	161, 746
EcoRII	CCWGG	1 cut(s)	658
EcoRV	GATATC	1 cut(s)	457
EcoT14I	CCWWGG	3 cut(s)	367, 1347, 1584
EcoT22I	ATGCAT	1 cut(s)	1968
EcoT38I	GRGCYC	2 cut(s)	490, 1475
ErhI	CCWWGG	3 cut(s)	367, 1347, 1584
Esp3I	CGTCTC	4 cut(s)	81, 113, 740, 1891
FaeI	CATG	9 cut(s)	228, 425, 605, 679, 773, 997, 1588, 1959, 1970
FalI	AAGNNNNNCTT	4 cut(s)	17, 49, 194, 226
FaqI	GGGAC	2 cut(s)	617, 2098
FatI	CATG	9 cut(s)	224, 421, 601, 675, 769, 993, 1584, 1955, 1966
FauI	CCCGC	1 cut(s)	942
FbaI	TGATCA	3 cut(s)	418, 424, 598
Fnu4HI	GCNGC	8 cut(s)	174, 177, 193, 448, 1168, 1237, 1390, 1661
FokI	GGATG	7 cut(s)	491, 907, 1102, 1521, 1865, 2017, 2228
FriOI	GRGCYC	2 cut(s)	490, 1475
Fsp4HI	GCNGC	8 cut(s)	174, 177, 193, 448, 1168, 1237, 1390, 1661
FspBI	CTAG	6 cut(s)	368, 1071, 1221, 1484, 1746, 2129
GlaI	GCGC	3 cut(s)	1156, 1244, 2105
GluI	GCNGC	8 cut(s)	174, 177, 193, 448, 1168, 1237, 1390, 1661
HaeII	RGCGCY	1 cut(s)	1158
HaeIII	GGCC	8 cut(s)	483, 615, 658, 663, 960, 1306, 1352, 1924
HapII	CCGG	5 cut(s)	129, 1254, 1283, 1525, 1771
HgaI	GACGC	3 cut(s)	695, 1666, 1700
HhaI	GCGC	3 cut(s)	1157, 1245, 2106
Hin1I	GRCGYC	3 cut(s)	438, 1692, 1761
Hin1II	CATG	9 cut(s)	228, 425, 605, 679, 773, 997, 1588, 1959, 1970
Hin6I	GCGC	3 cut(s)	1155, 1243, 2104
HinP1I	GCGC	3 cut(s)	1155, 1243, 2104
HincII	GTYRAC	3 cut(s)	222, 292, 442
HindII	GTYRAC	3 cut(s)	222, 292, 442
HindIII	AAGCTT	3 cut(s)	38, 1077, 1418
HinfI	GANTC	9 cut(s)	105, 510, 777, 1005, 1540, 1814, 1829, 1916, 2133
HpaI	GTTAAC	1 cut(s)	292
HpaII	CCGG	5 cut(s)	129, 1254, 1283, 1525, 1771
HphI	GGTGA	5 cut(s)	610, 923, 966, 1054, 1996
Hpy166II	GTNNAC	8 cut(s)	46, 222, 292, 442, 645, 856, 1405, 1656
Hpy188III	TCNNGA	7 cut(s)	116, 422, 965, 1087, 1568, 1757, 1818
Hpy8I	GTNNAC	8 cut(s)	46, 222, 292, 442, 645, 856, 1405, 1656
Hpy99I	CGWCG	5 cut(s)	137, 440, 689, 848, 1099
HpyAV	CCTTC	6 cut(s)	631, 648, 1372, 1472, 1570, 1815
HpyCH4III	ACNGT	8 cut(s)	578, 649, 890, 923, 1045, 2014, 2068, 2149
HpyCH4IV	ACGT	6 cut(s)	10, 90, 135, 294, 438, 1761
HpyF10VI	GCNNNNNNNGC	8 cut(s)	485, 676, 1154, 1217, 1242, 1398, 1489, 2103
HpyF3I	CTNAG	6 cut(s)	467, 850, 1158, 1356, 1697, 2162
HpySE526I	ACGT	6 cut(s)	10, 90, 135, 294, 438, 1761
Hsp92I	GRCGYC	3 cut(s)	438, 1692, 1761
Hsp92II	CATG	9 cut(s)	228, 425, 605, 679, 773, 997, 1588, 1959, 1970
HspAI	GCGC	3 cut(s)	1155, 1243, 2104
Ksp22I	TGATCA	3 cut(s)	418, 424, 598
KspAI	GTTAAC	1 cut(s)	292
KspI	CCGCGG	1 cut(s)	180
Kzo9I	GATC	7 cut(s)	418, 424, 598, 911, 967, 1098, 1939
LmnI	GCTCC	2 cut(s)	369, 2026
Lsp1109I	GCAGC	3 cut(s)	179, 459, 1401
LweI	GCATC	4 cut(s)	313, 827, 1080, 1543
MaeI	CTAG	6 cut(s)	368, 1071, 1221, 1484, 1746, 2129
MaeII	ACGT	6 cut(s)	10, 90, 135, 294, 438, 1761
MaeIII	GTNAC	4 cut(s)	295, 1115, 1126, 2074
MalI	GATC	7 cut(s)	420, 426, 600, 913, 969, 1100, 1941
MboI	GATC	7 cut(s)	418, 424, 598, 911, 967, 1098, 1939
MfeI	CAATTG	3 cut(s)	792, 2092, 2098
MflI	RGATCY	1 cut(s)	1939
MhlI	GDGCHC	5 cut(s)	96, 490, 1150, 1475, 2012
MlsI	TGGCCA	1 cut(s)	1352
MluNI	TGGCCA	1 cut(s)	1352
MlyI	GAGTC	2 cut(s)	504, 1838
Mox20I	TGGCCA	1 cut(s)	1352
Mph1103I	ATGCAT	1 cut(s)	1968
MscI	TGGCCA	1 cut(s)	1352
MslI	CAYNNNNRTG	4 cut(s)	276, 1124, 1190, 1589
Msp20I	TGGCCA	1 cut(s)	1352
MspA1I	CMGCKG	1 cut(s)	179
MspI	CCGG	5 cut(s)	129, 1254, 1283, 1525, 1771
MspR9I	CCNGG	3 cut(s)	660, 1526, 1772
MunI	CAATTG	3 cut(s)	792, 2092, 2098
Mva1269I	GAATGC	1 cut(s)	1968
MvaI	CCWGG	1 cut(s)	660
MvnI	CGCG	3 cut(s)	179, 1167, 1660
MwoI	GCNNNNNNNGC	8 cut(s)	485, 676, 1154, 1217, 1242, 1398, 1489, 2103
NciI	CCSGG	2 cut(s)	1526, 1772
NcoI	CCATGG	1 cut(s)	1584
NdeII	GATC	7 cut(s)	418, 424, 598, 911, 967, 1098, 1939
NlaIII	CATG	9 cut(s)	228, 425, 605, 679, 773, 997, 1588, 1959, 1970
NlaIV	GGNNCC	5 cut(s)	365, 580, 1025, 1548, 1923
NmeAIII	GCCGAG	2 cut(s)	209, 509
NmuCI	GTSAC	3 cut(s)	295, 1126, 2074
NsiI	ATGCAT	1 cut(s)	1968
NspI	RCATGY	2 cut(s)	228, 997
PagI	TCATGA	1 cut(s)	421
PceI	AGGCCT	2 cut(s)	658, 1306
PciI	ACATGT	1 cut(s)	993
PcsI	WCGNNNNNNNCGW	2 cut(s)	1103, 1107
PctI	GAATGC	1 cut(s)	1968
PfeI	GAWTC	7 cut(s)	105, 777, 1005, 1540, 1814, 1916, 2133
PkrI	GCNGC	8 cut(s)	175, 178, 194, 449, 1169, 1238, 1391, 1662
Ple19I	CGATCG	1 cut(s)	1101
PleI	GAGTC	2 cut(s)	504, 1837
PpsI	GAGTC	2 cut(s)	504, 1837
Ppu21I	YACGTR	1 cut(s)	11
PscI	ACATGT	1 cut(s)	993
PshAI	GACNNNNGTC	1 cut(s)	732
PshBI	ATTAAT	1 cut(s)	2019
PsiI	TTATAA	1 cut(s)	2193
Psp124BI	GAGCTC	1 cut(s)	1475
Psp6I	CCWGG	1 cut(s)	658
PspGI	CCWGG	1 cut(s)	658
PspN4I	GGNNCC	5 cut(s)	365, 580, 1025, 1548, 1923
PspPI	GGNCC	5 cut(s)	281, 662, 881, 959, 1922
PsuI	RGATCY	1 cut(s)	1939
PvuI	CGATCG	1 cut(s)	1101
RsaI	GTAC	9 cut(s)	13, 29, 206, 431, 925, 992, 1229, 1373, 1406
RsaNI	GTAC	9 cut(s)	12, 28, 205, 430, 924, 991, 1228, 1372, 1405
RseI	CAYNNNNRTG	4 cut(s)	276, 1124, 1190, 1589
SacI	GAGCTC	1 cut(s)	1475
SacII	CCGCGG	1 cut(s)	180
SatI	GCNGC	8 cut(s)	174, 177, 193, 448, 1168, 1237, 1390, 1661
Sau3AI	GATC	7 cut(s)	418, 424, 598, 911, 967, 1098, 1939
Sau96I	GGNCC	5 cut(s)	281, 662, 881, 959, 1922
ScaI	AGTACT	3 cut(s)	206, 431, 925
SchI	GAGTC	2 cut(s)	504, 1838
ScrFI	CCNGG	3 cut(s)	660, 1526, 1772
SduI	GDGCHC	5 cut(s)	96, 490, 1150, 1475, 2012
SfaNI	GCATC	4 cut(s)	313, 827, 1080, 1543
SfcI	CTRYAG	2 cut(s)	1231, 1408
Sfr303I	CCGCGG	1 cut(s)	180
SgrBI	CCGCGG	1 cut(s)	180
SinI	GGWCC	2 cut(s)	281, 881
SmiMI	CAYNNNNRTG	4 cut(s)	276, 1124, 1190, 1589
SmlI	CTYRAG	1 cut(s)	385
SmoI	CTYRAG	1 cut(s)	385
SnaBI	TACGTA	1 cut(s)	11
SpeI	ACTAGT	2 cut(s)	1070, 1745
SseBI	AGGCCT	2 cut(s)	658, 1306
SspI	AATATT	1 cut(s)	55
SspMI	CTAG	6 cut(s)	368, 1071, 1221, 1484, 1746, 2129
SstI	GAGCTC	1 cut(s)	1475
StuI	AGGCCT	2 cut(s)	658, 1306
StyD4I	CCNGG	3 cut(s)	658, 1524, 1770
StyI	CCWWGG	3 cut(s)	367, 1347, 1584
TaaI	ACNGT	8 cut(s)	578, 649, 890, 923, 1045, 2014, 2068, 2149
TaiI	ACGT	6 cut(s)	13, 93, 138, 297, 441, 1764
TaqI	TCGA	7 cut(s)	96, 516, 720, 1097, 1101, 1321, 1860
TaqII	GACCGA	2 cut(s)	114, 1315
TatI	WGTACW	6 cut(s)	204, 429, 923, 990, 1227, 1404
TauI	GCSGC	5 cut(s)	176, 179, 1170, 1239, 1663
TfiI	GAWTC	7 cut(s)	105, 777, 1005, 1540, 1814, 1916, 2133
TscAI	CASTG	6 cut(s)	379, 652, 1842, 2017, 2071, 2125
TseFI	GTSAC	3 cut(s)	295, 1126, 2074
TseI	GCWGC	3 cut(s)	192, 447, 1389
Tsp45I	GTSAC	3 cut(s)	295, 1126, 2074
TspDTI	ATGAA	3 cut(s)	613, 1174, 2125
TspGWI	ACGGA	2 cut(s)	159, 1641
TspRI	CASTG	6 cut(s)	379, 652, 1842, 2017, 2071, 2125
VpaK11BI	GGWCC	2 cut(s)	281, 881
VspI	ATTAAT	1 cut(s)	2019
XapI	RAATTY	6 cut(s)	151, 161, 746, 900, 1057, 1341
XceI	RCATGY	2 cut(s)	228, 997
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1557
XmaJI	CCTAGG	1 cut(s)	367
XspI	CTAG	6 cut(s)	368, 1071, 1221, 1484, 1746, 2129
ZraI	GACGTC	2 cut(s)	439, 1762
ZrmI	AGTACT	3 cut(s)	206, 431, 925
Zsp2I	ATGCAT	1 cut(s)	1968

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.