Rw1G040750

Lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0012656
Physical Location & Seq
Reverse (-)
67876752 .. 67886993
10242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G040750.1

Sequence Viewer

Length: 2706 bp
ATGTCTGACGTAGTTATGAAGCTGCTGCTCTTTTTGTCAGTTTTCCTGCCTCTCCCAAATCTCACACTTCCCACAAGTCGGAGCCGCCGAGACCCTCAGAGAAACCGAGGCATCATGAAGACCATTCTTTCGTCGGAGACCATGGACATTCCGGACGGGGTGAAGATCAAGGTCCACGCGAAGATCATCGAGGTGGAAGGCCCGCGCGGCAAGCTCGTCCGCAACTTCAAGCACCTCAACCTTGATTTCCAGCTCATCAAGGACGAGGAGACCGGCCAGCGCAAGCTCAAGATCGAGGCCTGGTTCGGCACTCGCAAGACCAGCGCCGCCATCAGGACCGCCCTCTCGCACGGGTACCGGTACAAGATGAGGTTCGTGTACGCTCATTTTCCCATCAACGCTTCGATCACTAATGGAGCCAAGTCGATTGAGATCAGGAACTTTCTCGGTGAGAAGAAGGTGAGAAAGGTGGATATGCTTGATGGTGTTTCTATCCTCCGATCTGAGAAGGTGAAGGATGAGCTGATATTGGATGGCAATGATATTGAACTTGTTTCTCGGTCTTGTGCTCTGATCAACCAGAAATGCCATGTCAAGAACAAGGATATCAGGAAGTTTCTTGATGGTATCTATCTTCACAACCTCAACTGCAATCAAATCCAAGCTCTTTCAATAATGAGTTGCTTCAACTGGTTGCTCTATTTAGCTGCTGCTTTGGCCGTGATACGCAACGAGCTTCTAGTTGATTCAGCTAGTGACAAGAAGCACCACAGCGGACCAGTTGTTAATGTGACCAAGCACCTCATGTTCCCAGATTTCAACCTCTCCTACAACCCCCGAATCTTGAACGATGTAAAGCTCTTAGGCAGTGCCAAGCTTGCTTCAAAAACCAGTGCTATACAAATCCCCGACGAGTCTCATGTCACTGATCTCAGACACCAAGCCGGCCGAGCCATCTACTCTTCTTCAATCCGACTCCTGGACCCTCACACCGAAACCCCTGCCTCTTTCGAAACAACCTTCTCTTTTCAATTCCACAATGCCTCTTCATCTTCACCTGAACGAATCCAAACTACTCAAAGCGGTGGTTATGGTAATGGCGGCAGTGGACTAGCCTTCGTCATCGTCCCGGATGAGTTCACAGTTGGCAGACCTGGTGCTTGGCTTGCCATGCTCAACGATGCTTGTGAAGATGACTACAAGGTCATAGCAATCGAATTCGACACACGTTTGAATCCAGAGTTTGGAGACCCTAATGATAACCATGTAGGTATCAACTTGGGGACTATTATCTCCACTGAAACAATCAACGCTTCTGATGTTGGCGTCTTCCTCAACGACGGTTCAGTCCACCGAGCTTGGATAAGCTACAACGGCTCACGGCGTTGGATGGAAATTCGTTTGGGATCAGACGACGGAAGTTACCCTTCAAAGCCAGTCTTCTCCGGATCTCTTGACCTCTCACCGTTTCTGAATGAGTACATGTTCGTTGGATTTTCGGCTTCCACAGGCAATCACACCCAAATCCACAGCGTGCTTTCTTGGAACTTCACTTCTGTTAGTCAAGCTTTTCTTCGTGTGCCGTCAATGGAGACATGTGAGAGCAAGATTGTTGTTACTGAAAACTCTCGTGCTGAGCCTCCCGGCACGTTCTTGATCTTTATAGCTGTGCTGGTACTTGCCATAGCTGTTATGCTTAGCCTTTACTATAACAGCAAGCGTAGAAGTGCAAATTCGGATAGTGCTAGTATAGTGTTGCCGGAGAAGAAGCAGAGACCGAGGCCTCCAAACAAGCCTAGAAGCTTCACAATCTCGGAGATTTCATTGGCGACTAGGTCTTTCGGAGAGCTAGAGATACTTGGAGGTGGATCAAAAGGGGTGTGCTACCGAGGGAAGCTTTCGAACGGATGTCAAGTGGCAGTGAAGCGGTTTTCGCCTCAGTTTATGAACTCACAAGGGTTGGATAGGAGGAGATTGATGAAGGAAATCAAGGGGATTAGCAGGGTTAGACACCCGAACTTAGTTTCCATTAGAGGATGGTGTCAGGACAAGAAAGAGACTATGGTTGTGTATGAGTTTTTCTCCAATGGAAGCCTTGATAAGTGGCTGTTTGGTGTTGGTGTTCTTCCGTGGACTCGGAGGTTCAAGGTTGTGAGAGATGTGGCTGAAGCACTCAGTTACTTGCACTCCAATCAATTGGCGCACAAGAACTTGAAGACCACCAGTGTCTTTCTTGATGTTAGTTTCCGGGCTGTTCTAGGAGACTTTGGGTTTGTGCTATGTGGAGCAGAGTCGAGAAGGTTTGAATCGACGGTGAGTCAAAGCGCCGATGTGTTTGAATTCGGCCTCTTTGTGTTGGAAGTAGTGGGAGGGAGGAAGAGGTTGGAGGCAGAGTTGGGTGAATTGGAGGAGAGGGACTTGCTGGACTTCATCTGGAGAATGCATGAAATTGGTGAAATGAGGAGGGTTGTGGATAAGAGAATGGGTGCAGTGATCAATTTGGATCAAGCAATTAGGGTTTTGGAGATTGGATTGCTTTGCACACTGAATGCGAGCAAAGGGAGGCCTAGTATGGAGCAAGTTGTGGAGTTTCTTTGCATGGAGAGGCCAATCCCTGAGTTGCCACAGACTCGACCTATTGCTTTGTTCCCCTACAACAGCACCAATGCTCTCTGTACAGGTTATTCTTGTGCTCCTTTCAAATGA

Protein Analysis

901

Amino Acids

100.95

Weight (kDa)

9.12

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6 PF00347 140 - 209 4.4e-10 Ribosomal protein L6
Lectin_legB PF00139 283 - 521 4.1e-51 Legume lectin domain
Lectin_L-type_dom PF18483 289 - 511 8.9e-09 Legume lectin beta-barrel domain
Pkinase PF00069 616 - 760 4.2e-22 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 618 - 758 1.9e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g33120
malus_domestica MD01G1230400.v1.1
prunus_persica Prupe.2G321100_v2.0.a1
pyrus_communis pycom01g23840
rosa_chinensis RchiOBHm_Chr1g0334661 RchiOBHm_Chr1g0383191
rosa_laevigata RLG00000026124
rosa_multiflora Rmu_sc0008563.1_g000003
rosa_roxburghii Rroxscaffold_4G00277520
rosa_rugosa Rorug01G0126300 Rorug01G0439400
rosa_samantha Rh1AG139900 Rh1AG465000 Rh1BG422400 Rh1CG131800 Rh1CG435600 Rh1DG143800 Rh1DG455100
rosa_wichuraiana Rw1G040750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1202, 1346
Acc65I GGTACC 1 cut(s) 354
AccB1I GGYRCC 1 cut(s) 354
AccB7I CCANNNNNTGG 1 cut(s) 1244
AccII CGCG 3 cut(s) 179, 205, 207
AccIII TCCGGA 2 cut(s) 151, 1445
AclWI GGATC 4 cut(s) 1414, 1456, 1876, 2511
AcoI YGGCCR 3 cut(s) 274, 717, 946
AcsI RAATTY 4 cut(s) 1217, 1395, 1732, 2339
AcuI CTGAAG 1 cut(s) 2187
AcyI GRCGYC 1 cut(s) 1326
AdeI CACNNNGTG 1 cut(s) 1534
AfaI GTAC 6 cut(s) 356, 362, 380, 1481, 1677, 2677
AfiI CCNNNNNNNGG 4 cut(s) 78, 333, 979, 1244
AflIII ACRYGT 3 cut(s) 1226, 1482, 1595
AgeI ACCGGT 1 cut(s) 357
AhdI GACNNNNNGTC 1 cut(s) 2316
AjnI CCWGG 3 cut(s) 299, 978, 1153
AloI GAACNNNNNNTCC 2 cut(s) 2009, 2041
Alw21I GWGCWC 2 cut(s) 571, 2695
Alw26I GTCTC 9 cut(s) 84, 131, 263, 921, 1242, 1586, 1768, 2051, 2256
AlwI GGATC 4 cut(s) 1414, 1456, 1876, 2511
Aor13HI TCCGGA 2 cut(s) 151, 1445
AoxI GGCC 9 cut(s) 199, 274, 297, 717, 946, 1781, 2344, 2564, 2606
ApeKI GCWGC 4 cut(s) 22, 25, 707, 710
ApoI RAATTY 4 cut(s) 1217, 1395, 1732, 2339
ArsI GACNNNNNNTTYG 4 cut(s) 112, 144, 2255, 2287
AsiGI ACCGGT 1 cut(s) 357
Asp718I GGTACC 1 cut(s) 354
AspLEI GCGC 5 cut(s) 207, 282, 326, 2203, 2327
AspS9I GGNCC 5 cut(s) 172, 200, 336, 776, 982
AsuC2I CCSGG 3 cut(s) 1130, 1644, 2249
AsuHPI GGTGA 9 cut(s) 172, 461, 472, 523, 1047, 1455, 2326, 2411, 2465
AsuII TTCGAA 2 cut(s) 1011, 1901
AvaII GGWCC 4 cut(s) 172, 336, 776, 982
BanI GGYRCC 1 cut(s) 354
BauI CACGAG 1 cut(s) 1629
BbsI GAAGAC 4 cut(s) 125, 1321, 1432, 2222
Bbv12I GWGCWC 2 cut(s) 571, 2695
BbvI GCAGC 4 cut(s) 9, 12, 694, 697
BccI CCATC 8 cut(s) 338, 401, 476, 527, 617, 962, 1384, 2031
BceAI ACGGC 4 cut(s) 704, 1390, 1397, 1567
BcgI CGANNNNNNTGC 2 cut(s) 983, 1017
BciT130I CCWGG 3 cut(s) 301, 980, 1155
BclI TGATCA 2 cut(s) 573, 2493
BcnI CCSGG 3 cut(s) 1130, 1644, 2249
BcoDI GTCTC 9 cut(s) 84, 131, 263, 921, 1242, 1586, 1768, 2051, 2256
BfaI CTAG 9 cut(s) 740, 753, 1112, 1746, 1797, 1833, 1850, 2258, 2568
BfoI RGCGCY 2 cut(s) 327, 2328
BglI GCCNNNNNGGC 1 cut(s) 207
BisI GCNGC 8 cut(s) 23, 26, 85, 208, 327, 708, 711, 1102
BlpI GCTNAGC 2 cut(s) 1635, 1697
BlsI GCNGC 8 cut(s) 24, 27, 86, 209, 328, 709, 712, 1103
Bme1390I CCNGG 6 cut(s) 301, 980, 1130, 1155, 1644, 2249
Bme18I GGWCC 4 cut(s) 172, 336, 776, 982
BmeRI GACNNNNNGTC 1 cut(s) 2316
BmgT120I GGNCC 5 cut(s) 172, 200, 336, 776, 982
BmiI GGNNCC 4 cut(s) 83, 356, 418, 984
BmrFI CCNGG 6 cut(s) 301, 980, 1130, 1155, 1644, 2249
BmsI GCATC 2 cut(s) 120, 1171
BpiI GAAGAC 4 cut(s) 125, 1321, 1432, 2222
BplI GAGNNNNNCTC 2 cut(s) 2066, 2098
BpmI CTGGAG 1 cut(s) 2455
Bpu1102I GCTNAGC 2 cut(s) 1635, 1697
Bpu14I TTCGAA 2 cut(s) 1011, 1901
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 3 cut(s) 1130, 1644, 2249
BsaBI GATNNNNATC 1 cut(s) 431
BsaHI GRCGYC 1 cut(s) 1326
BsaI GGTCTC 5 cut(s) 84, 131, 263, 1242, 1768
BsaJI CCNNGG 5 cut(s) 106, 141, 1778, 1888, 2129
BsaWI WCCGGW 3 cut(s) 151, 357, 1445
BsaXI ACNNNNNCTCC 6 cut(s) 2171, 2201, 2378, 2408, 2554, 2584
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 333, 979, 1244
Bse118I RCCGGY 3 cut(s) 272, 357, 944
Bse1I ACTGG 5 cut(s) 695, 779, 891, 1436, 2223
Bse3DI GCAATG 1 cut(s) 544
Bse8I GATNNNNATC 1 cut(s) 431
BseAI TCCGGA 2 cut(s) 151, 1445
BseBI CCWGG 3 cut(s) 301, 980, 1155
BseDI CCNNGG 5 cut(s) 106, 141, 1778, 1888, 2129
BseGI GGATG 6 cut(s) 523, 538, 1138, 1395, 1913, 2042
BseJI GATNNNNATC 1 cut(s) 431
BseLI CCNNNNNNNGG 4 cut(s) 78, 333, 979, 1244
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 7 cut(s) 110, 495, 946, 1626, 1952, 2188, 2607
BseNI ACTGG 5 cut(s) 695, 779, 891, 1436, 2223
BseRI GAGGAG 4 cut(s) 281, 1984, 2423, 2476
BseX3I CGGCCG 1 cut(s) 946
BseXI GCAGC 4 cut(s) 9, 12, 694, 697
BsgI GTGCAG 1 cut(s) 2508
Bsh1236I CGCG 3 cut(s) 179, 205, 207
Bsh1285I CGRYCG 1 cut(s) 949
BshFI GGCC 9 cut(s) 201, 276, 299, 719, 948, 1783, 2346, 2566, 2608
BshNI GGYRCC 1 cut(s) 354
BshTI ACCGGT 1 cut(s) 357
BsiEI CGRYCG 1 cut(s) 949
BsiHKAI GWGCWC 2 cut(s) 571, 2695
BsiSI CCGG 9 cut(s) 152, 273, 358, 945, 1130, 1446, 1644, 1760, 2248
BslFI GGGAC 3 cut(s) 1112, 1297, 2429
BslI CCNNNNNNNGG 4 cut(s) 78, 333, 979, 1244
BsmAI GTCTC 9 cut(s) 84, 131, 263, 921, 1242, 1586, 1768, 2051, 2256
BsmFI GGGAC 3 cut(s) 1112, 1297, 2429
BsmI GAATGC 2 cut(s) 2445, 2554
BsnI GGCC 9 cut(s) 201, 276, 299, 719, 948, 1783, 2346, 2566, 2608
Bso31I GGTCTC 5 cut(s) 84, 131, 263, 1242, 1768
Bsp119I TTCGAA 2 cut(s) 1011, 1901
Bsp1286I GDGCHC 2 cut(s) 571, 2695
Bsp13I TCCGGA 2 cut(s) 151, 1445
Bsp1407I TGTACA 1 cut(s) 2675
Bsp1720I GCTNAGC 2 cut(s) 1635, 1697
Bsp19I CCATGG 1 cut(s) 141
BspANI GGCC 9 cut(s) 201, 276, 299, 719, 948, 1783, 2346, 2566, 2608
BspCNI CTCAG 7 cut(s) 109, 496, 945, 1627, 1951, 2187, 2608
BspEI TCCGGA 2 cut(s) 151, 1445
BspFNI CGCG 3 cut(s) 179, 205, 207
BspHI TCATGA 1 cut(s) 114
BspLI GGNNCC 4 cut(s) 83, 356, 418, 984
BspPI GGATC 4 cut(s) 1414, 1456, 1876, 2511
BspT104I TTCGAA 2 cut(s) 1011, 1901
BspT107I GGYRCC 1 cut(s) 354
BspTNI GGTCTC 5 cut(s) 84, 131, 263, 1242, 1768
BsrDI GCAATG 1 cut(s) 544
BsrFI RCCGGY 3 cut(s) 272, 357, 944
BsrGI TGTACA 1 cut(s) 2675
BsrI ACTGG 5 cut(s) 695, 779, 891, 1436, 2223
BssAI RCCGGY 3 cut(s) 272, 357, 944
BssECI CCNNGG 5 cut(s) 106, 141, 1778, 1888, 2129
BssNI GRCGYC 1 cut(s) 1326
BssSI CACGAG 1 cut(s) 1629
BssT1I CCWWGG 1 cut(s) 141
Bst2BI CACGAG 1 cut(s) 1629
Bst2UI CCWGG 3 cut(s) 301, 980, 1155
Bst4CI ACNGT 4 cut(s) 1144, 1343, 1467, 2314
Bst6I CTCTTC 3 cut(s) 967, 1051, 2372
BstACI GRCGYC 1 cut(s) 1326
BstAUI TGTACA 1 cut(s) 2675
BstBI TTCGAA 2 cut(s) 1011, 1901
BstDSI CCRYGG 2 cut(s) 141, 2129
BstF5I GGATG 6 cut(s) 523, 538, 1138, 1395, 1913, 2042
BstFNI CGCG 3 cut(s) 179, 205, 207
BstH2I RGCGCY 2 cut(s) 327, 2328
BstHHI GCGC 5 cut(s) 207, 282, 326, 2203, 2327
BstMAI GTCTC 9 cut(s) 84, 131, 263, 921, 1242, 1586, 1768, 2051, 2256
BstMCI CGRYCG 1 cut(s) 949
BstNI CCWGG 3 cut(s) 301, 980, 1155
BstNSI RCATGY 2 cut(s) 1486, 1599
BstSCI CCNGG 6 cut(s) 299, 978, 1128, 1153, 1642, 2247
BstUI CGCG 3 cut(s) 179, 205, 207
BstV1I GCAGC 4 cut(s) 9, 12, 694, 697
BstV2I GAAGAC 4 cut(s) 125, 1321, 1432, 2222
BstX2I RGATCY 1 cut(s) 1448
BstXI CCANNNNNNTGG 1 cut(s) 2197
BstYI RGATCY 1 cut(s) 1448
BstZI CGGCCG 1 cut(s) 946
BsuRI GGCC 9 cut(s) 201, 276, 299, 719, 948, 1783, 2346, 2566, 2608
BtgI CCRYGG 2 cut(s) 141, 2129
BtsCI GGATG 6 cut(s) 523, 538, 1138, 1395, 1913, 2042
BtsI GCAGTG 4 cut(s) 874, 1111, 1926, 2496
BtsIMutI CAGTG 9 cut(s) 874, 898, 924, 1111, 1296, 1926, 2230, 2496, 2543
CciI TCATGA 1 cut(s) 114
CfoI GCGC 5 cut(s) 207, 282, 326, 2203, 2327
Cfr10I RCCGGY 3 cut(s) 272, 357, 944
Cfr13I GGNCC 5 cut(s) 172, 200, 336, 776, 982
CseI GACGC 1 cut(s) 1315
CsiI ACCWGGT 1 cut(s) 1153
Csp6I GTAC 6 cut(s) 355, 361, 379, 1480, 1676, 2676
CspAI ACCGGT 1 cut(s) 357
CspCI CAANNNNNGTGG 2 cut(s) 1340, 1375
CviQI GTAC 6 cut(s) 355, 361, 379, 1480, 1676, 2676
DraIII CACNNNGTG 1 cut(s) 1534
DrdI GACNNNNNNGTC 2 cut(s) 1202, 1346
DriI GACNNNNNGTC 1 cut(s) 2316
DseDI GACNNNNNNGTC 2 cut(s) 1202, 1346
EaeI YGGCCR 3 cut(s) 274, 717, 946
EagI CGGCCG 1 cut(s) 946
Eam1104I CTCTTC 3 cut(s) 967, 1051, 2372
Eam1105I GACNNNNNGTC 1 cut(s) 2316
EarI CTCTTC 3 cut(s) 967, 1051, 2372
EclXI CGGCCG 1 cut(s) 946
Eco130I CCWWGG 1 cut(s) 141
Eco147I AGGCCT 3 cut(s) 299, 1783, 2566
Eco31I GGTCTC 5 cut(s) 84, 131, 263, 1242, 1768
Eco32I GATATC 1 cut(s) 607
Eco47I GGWCC 4 cut(s) 172, 336, 776, 982
Eco52I CGGCCG 1 cut(s) 946
Eco57I CTGAAG 1 cut(s) 2187
EcoRI GAATTC 2 cut(s) 1217, 2339
EcoRII CCWGG 3 cut(s) 299, 978, 1153
EcoRV GATATC 1 cut(s) 607
EcoT14I CCWWGG 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 2445
ErhI CCWWGG 1 cut(s) 141
FalI AAGNNNNNCTT 6 cut(s) 1411, 1443, 1424, 1456, 1557, 1589
FaqI GGGAC 3 cut(s) 1112, 1297, 2429
FauI CCCGC 1 cut(s) 210
FbaI TGATCA 2 cut(s) 573, 2493
Fnu4HI GCNGC 8 cut(s) 23, 26, 85, 208, 327, 708, 711, 1102
FokI GGATG 6 cut(s) 530, 545, 1145, 1402, 1920, 2049
Fsp4HI GCNGC 8 cut(s) 23, 26, 85, 208, 327, 708, 711, 1102
FspBI CTAG 9 cut(s) 740, 753, 1112, 1746, 1797, 1833, 1850, 2258, 2568
GlaI GCGC 5 cut(s) 206, 281, 325, 2202, 2326
GluI GCNGC 8 cut(s) 23, 26, 85, 208, 327, 708, 711, 1102
GsuI CTGGAG 1 cut(s) 2455
HaeII RGCGCY 2 cut(s) 327, 2328
HaeIII GGCC 9 cut(s) 201, 276, 299, 719, 948, 1783, 2346, 2566, 2608
HapII CCGG 9 cut(s) 152, 273, 358, 945, 1130, 1446, 1644, 1760, 2248
HgaI GACGC 1 cut(s) 1315
HhaI GCGC 5 cut(s) 207, 282, 326, 2203, 2327
Hin1I GRCGYC 1 cut(s) 1326
Hin6I GCGC 5 cut(s) 205, 280, 324, 2201, 2325
HinP1I GCGC 5 cut(s) 205, 280, 324, 2201, 2325
HindIII AAGCTT 4 cut(s) 875, 1566, 1801, 1895
HpaII CCGG 9 cut(s) 152, 273, 358, 945, 1130, 1446, 1644, 1760, 2248
HphI GGTGA 9 cut(s) 172, 461, 472, 523, 1047, 1455, 2326, 2411, 2465
Hpy166II GTNNAC 6 cut(s) 175, 379, 1109, 1140, 1351, 2133
Hpy8I GTNNAC 6 cut(s) 175, 379, 1109, 1140, 1351, 2133
Hpy99I CGWCG 5 cut(s) 136, 914, 1343, 1418, 2314
HpyAV CCTTC 9 cut(s) 191, 451, 502, 508, 1030, 1126, 1437, 1975, 2292
HpyCH4III ACNGT 4 cut(s) 1144, 1343, 1467, 2314
HpyCH4IV ACGT 3 cut(s) 9, 1228, 1649
HpyCH4V TGCA 7 cut(s) 651, 1730, 2185, 2443, 2489, 2541, 2598
HpySE526I ACGT 3 cut(s) 9, 1228, 1649
Hsp92I GRCGYC 1 cut(s) 1326
HspAI GCGC 5 cut(s) 205, 280, 324, 2201, 2325
Kpn2I TCCGGA 2 cut(s) 151, 1445
KpnI GGTACC 1 cut(s) 358
KroI GCCGGC 1 cut(s) 944
KroNI GCCGGC 1 cut(s) 946
Ksp22I TGATCA 2 cut(s) 573, 2493
LmnI GCTCC 5 cut(s) 81, 416, 2285, 2575, 2698
Lsp1109I GCAGC 4 cut(s) 9, 12, 694, 697
LweI GCATC 2 cut(s) 120, 1171
MabI ACCWGGT 1 cut(s) 1153
MaeI CTAG 9 cut(s) 740, 753, 1112, 1746, 1797, 1833, 1850, 2258, 2568
MaeII ACGT 3 cut(s) 9, 1228, 1649
MaeIII GTNAC 6 cut(s) 755, 790, 922, 1421, 1615, 2177
MfeI CAATTG 1 cut(s) 2195
MflI RGATCY 1 cut(s) 1448
MhlI GDGCHC 2 cut(s) 571, 2695
MlyI GAGTC 6 cut(s) 923, 969, 2128, 2300, 2326, 2623
MmeI TCCRAC 8 cut(s) 59, 114, 997, 1367, 1471, 1941, 2337, 2364
Mph1103I ATGCAT 1 cut(s) 2445
MroI TCCGGA 2 cut(s) 151, 1445
MroNI GCCGGC 1 cut(s) 944
MseI TTAA 1 cut(s) 786
MslI CAYNNNNRTG 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 774
MspI CCGG 9 cut(s) 152, 273, 358, 945, 1130, 1446, 1644, 1760, 2248
MspR9I CCNGG 6 cut(s) 301, 980, 1130, 1155, 1644, 2249
MunI CAATTG 1 cut(s) 2195
Mva1269I GAATGC 2 cut(s) 2445, 2554
MvaI CCWGG 3 cut(s) 301, 980, 1155
MvnI CGCG 3 cut(s) 179, 205, 207
NaeI GCCGGC 1 cut(s) 946
NciI CCSGG 3 cut(s) 1130, 1644, 2249
NcoI CCATGG 1 cut(s) 141
NgoMIV GCCGGC 1 cut(s) 944
NlaIV GGNNCC 4 cut(s) 83, 356, 418, 984
NmeAIII GCCGAG 2 cut(s) 113, 974
NmuCI GTSAC 3 cut(s) 755, 790, 922
NsiI ATGCAT 1 cut(s) 2445
NspI RCATGY 2 cut(s) 1486, 1599
NspV TTCGAA 2 cut(s) 1011, 1901
PagI TCATGA 1 cut(s) 114
PceI AGGCCT 3 cut(s) 299, 1783, 2566
PciI ACATGT 2 cut(s) 1482, 1595
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PctI GAATGC 2 cut(s) 2445, 2554
PdiI GCCGGC 1 cut(s) 946
PfeI GAWTC 5 cut(s) 746, 840, 1065, 1234, 2306
PflFI GACNNNGTC 1 cut(s) 1834
PflMI CCANNNNNTGG 1 cut(s) 1244
PfoI TCCNGGA 2 cut(s) 978, 1128
PinAI ACCGGT 1 cut(s) 357
PkrI GCNGC 8 cut(s) 24, 27, 86, 209, 328, 709, 712, 1103
PleI GAGTC 6 cut(s) 922, 969, 2128, 2299, 2325, 2623
PpsI GAGTC 6 cut(s) 922, 969, 2128, 2299, 2325, 2623
PscI ACATGT 2 cut(s) 1482, 1595
Psp6I CCWGG 3 cut(s) 299, 978, 1153
PspGI CCWGG 3 cut(s) 299, 978, 1153
PspN4I GGNNCC 4 cut(s) 83, 356, 418, 984
PspPI GGNCC 5 cut(s) 172, 200, 336, 776, 982
PsuI RGATCY 1 cut(s) 1448
PsyI GACNNNGTC 1 cut(s) 1834
RsaI GTAC 6 cut(s) 356, 362, 380, 1481, 1677, 2677
RsaNI GTAC 6 cut(s) 355, 361, 379, 1480, 1676, 2676
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 1 cut(s) 786
SatI GCNGC 8 cut(s) 23, 26, 85, 208, 327, 708, 711, 1102
Sau96I GGNCC 5 cut(s) 172, 200, 336, 776, 982
SchI GAGTC 6 cut(s) 923, 969, 2128, 2300, 2326, 2623
ScrFI CCNGG 6 cut(s) 301, 980, 1130, 1155, 1644, 2249
SduI GDGCHC 2 cut(s) 571, 2695
SexAI ACCWGGT 1 cut(s) 1153
SfaNI GCATC 2 cut(s) 120, 1171
SfuI TTCGAA 2 cut(s) 1011, 1901
SinI GGWCC 4 cut(s) 172, 336, 776, 982
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SseBI AGGCCT 3 cut(s) 299, 1783, 2566
SspMI CTAG 9 cut(s) 740, 753, 1112, 1746, 1797, 1833, 1850, 2258, 2568
StuI AGGCCT 3 cut(s) 299, 1783, 2566
StyD4I CCNGG 6 cut(s) 299, 978, 1128, 1153, 1642, 2247
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 4 cut(s) 1144, 1343, 1467, 2314
TaiI ACGT 3 cut(s) 12, 1231, 1652
TaqII GACCGA 2 cut(s) 549, 1792
TatI WGTACW 2 cut(s) 1479, 2675
TauI GCSGC 4 cut(s) 87, 210, 329, 1104
TfiI GAWTC 5 cut(s) 746, 840, 1065, 1234, 2306
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 9 cut(s) 874, 898, 931, 1111, 1303, 1926, 2230, 2496, 2550
TseFI GTSAC 3 cut(s) 755, 790, 922
TseI GCWGC 4 cut(s) 22, 25, 707, 710
Tsp45I GTSAC 3 cut(s) 755, 790, 922
TspDTI ATGAA 8 cut(s) 32, 131, 1038, 1812, 1961, 1994, 2419, 2460
TspGWI ACGGA 3 cut(s) 1431, 1920, 2118
TspRI CASTG 9 cut(s) 874, 898, 931, 1111, 1303, 1926, 2230, 2496, 2550
Tth111I GACNNNGTC 1 cut(s) 1834
Van91I CCANNNNNTGG 1 cut(s) 1244
VpaK11BI GGWCC 4 cut(s) 172, 336, 776, 982
XapI RAATTY 4 cut(s) 1217, 1395, 1732, 2339
XceI RCATGY 2 cut(s) 1486, 1599
XspI CTAG 9 cut(s) 740, 753, 1112, 1746, 1797, 1833, 1850, 2258, 2568
Zsp2I ATGCAT 1 cut(s) 2445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.