MD01G1233700.v1.1

Belongs to the phospholipid scramblase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0017071
Physical Location & Seq
Reverse (-)
32148324 .. 32153778
5455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1233700.v1.1.491

Sequence Viewer

Length: 1287 bp
ATGAATTGGACAAGGTTTTCCACCAAGGTTTACAAAACTTGTGCGGCAAAATCCAAATGTGGATCCTCATTCTGTCATATCAGAAAACCCCATCAGAATGCTATTGAAGACACCAAAAGCATTTGGGTCCACTGTGGTTTTCAGTCTCAAAGAGGAAGTCTACCTGCTACTTCATGCCCTCAGGATGGATCCTGTGTTGCTACTGTAGCTAAAGATTTTGAGCAGCGGTTGATTGGAGCAAGAAATGTAATTTCAAAGTTTAAAGGAAGTTCCATGTGGTCTGAACAGAGACCGGTATCTTTGTCTCGTCAGTTTGGACACAAGGCTGGTACTGGCGGTGATCCTGAATTGACTAGGGACTTCCTTGTACAGCTCTGGGTTGCAGATAGGAAAATGGAACAGTCTAAAAGAAAGAGAAGAGGGAAAGCTGTTAAGTATATTAAAAAATCACCGTACCATTATTCATCCTATGGATGGTATCCAAGTGCACCTTTCAGTCAAGCAGTCTCTTATGATGAGGATGAACCCAATATGACACAACCGCCCCCAAGCCAATCCATCTCAGGATATCTGAAGCCAGAATCTCCAGAAGAAGCCCAGGTGATGCCTCTTCTTGCTAGATCAAATCTGCTGATTACTAGGGATATAGAGTGGGCAAATCTTGCTTTTGGATTTGAGCAGGAAAATCGGTATGGAATAGTGGATGTGTGCTACCCAGAGTCACCTGTTGGTTTGATTCGTGAGCAGAGTCATGTTATTGCTAGACAGTTGCTTCGTCTCAGGCGCCCTTTTGTTGCTCTTGTAACTGATGCCATGGGTAATGAGCTCTTTAGGGTTCGCAGGCCTTTTTGGTGGATAACCAGCTCAATTTATGTAGAGATCAATGGTAAGGAAATCGGTGTGGTTCACAGACGATGGCATCTTTGGAGGAGGGTGTATGATTTGTACCTAGGGGATAAACAGTTTGCAGTTGTAGAAAATCCTGGCTTCTGGCATTGGACTTTTACTTTGAAGGACATTGATGGGGGAGTTTTGGGTGAGATAGATCGTGAGTGGAGGGGTTTTGGCTTTGAGATTTTCACTGATGCAGGGCAATATGTGATTCGGTTTGGGAGTTCTGATCCCAGCTCTATTACTGGTCCTTCTAGAATTGAAGAGTTGGAAGTTGTTCGACCACTGACTCTGCTAGAGAGAGCTGTAACTGTTGCTCTTGCCATATCGTTGGACAATGACTATTTCTCACGACACGGTGGATGGGGATTGCCATTTTATGAAGTAGGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Scramblase

Protein Analysis

429

Amino Acids

48.95

Weight (kDa)

8.92

Isoelectric Point (pI)

60.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Scramblase PF03803 191 - 415 3.6e-60 Scramblase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04940
fragaria_vesca FvH4_7g33592
malus_domestica MD01G1233700.v1.1 MD07G1305800.v1.1
prunus_persica Prupe.2G323900_v2.0.a1
pyrus_communis pycom01g24200
rosa_chinensis RchiOBHm_Chr1g0383851
rosa_laevigata RLG00000026091
rosa_samantha Rh1AG468500 Rh1BG426200 Rh1CG438900 Rh1DG459000
rosa_wichuraiana Rw1G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 783
AccI GTMKAC 1 cut(s) 160
AciI CCGC 4 cut(s) 44, 226, 336, 542
AclWI GGATC 6 cut(s) 57, 70, 183, 196, 335, 1115
AcuI CTGAAG 1 cut(s) 593
AcyI GRCGYC 1 cut(s) 784
AfaI GTAC 4 cut(s) 331, 369, 455, 947
AfiI CCNNNNNNNGG 2 cut(s) 185, 474
AgeI ACCGGT 1 cut(s) 292
AgsI TTSAA 4 cut(s) 107, 255, 1012, 1154
AjnI CCWGG 2 cut(s) 597, 982
AluBI AGCT 7 cut(s) 209, 373, 428, 826, 864, 1128, 1196
AluI AGCT 7 cut(s) 209, 373, 428, 826, 864, 1128, 1196
Alw21I GWGCWC 2 cut(s) 490, 828
Alw26I GTCTC 5 cut(s) 150, 283, 309, 511, 782
Alw44I GTGCAC 1 cut(s) 486
AlwI GGATC 6 cut(s) 57, 70, 183, 196, 335, 1115
AoxI GGCC 1 cut(s) 842
ApaLI GTGCAC 1 cut(s) 486
ApeKI GCWGC 1 cut(s) 223
ArsI GACNNNNNNTTYG 6 cut(s) 142, 174, 756, 788, 991, 1023
AsiGI ACCGGT 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 1167
AspA2I CCTAGG 1 cut(s) 949
AspLEI GCGC 1 cut(s) 786
AspS9I GGNCC 2 cut(s) 127, 1139
AsuHPI GGTGA 5 cut(s) 350, 441, 613, 714, 1049
AvaII GGWCC 2 cut(s) 127, 1139
AvrII CCTAGG 1 cut(s) 949
AxyI CCTNAGG 1 cut(s) 180
BaeGI GKGCMC 1 cut(s) 490
BamHI GGATCC 2 cut(s) 62, 188
BanI GGYRCC 1 cut(s) 783
BanII GRGCYC 1 cut(s) 828
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 2 cut(s) 490, 828
BbvI GCAGC 1 cut(s) 235
BccI CCATC 7 cut(s) 99, 179, 468, 566, 909, 1016, 1248
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BciT130I CCWGG 2 cut(s) 599, 984
BciVI GTATCC 1 cut(s) 489
BcoDI GTCTC 5 cut(s) 150, 283, 309, 511, 782
BfaI CTAG 7 cut(s) 354, 618, 639, 762, 950, 1146, 1187
BfmI CTRYAG 1 cut(s) 204
BfoI RGCGCY 1 cut(s) 787
BfuAI ACCTGC 1 cut(s) 172
BfuI GTATCC 1 cut(s) 489
BisI GCNGC 2 cut(s) 45, 224
BlnI CCTAGG 1 cut(s) 949
BlsI GCNGC 2 cut(s) 46, 225
Bme1390I CCNGG 2 cut(s) 599, 984
Bme18I GGWCC 2 cut(s) 127, 1139
BmgT120I GGNCC 2 cut(s) 127, 1139
BmiI GGNNCC 4 cut(s) 64, 128, 190, 785
BmrFI CCNGG 2 cut(s) 599, 984
BmsI GCATC 4 cut(s) 594, 799, 928, 1075
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 570
BsaHI GRCGYC 1 cut(s) 784
BsaI GGTCTC 1 cut(s) 283
BsaJI CCNNGG 4 cut(s) 24, 597, 813, 949
BsaWI WCCGGW 1 cut(s) 292
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 474
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 2 cut(s) 337, 1141
Bse21I CCTNAGG 1 cut(s) 180
BseBI CCWGG 2 cut(s) 599, 984
BseDI CCNNGG 4 cut(s) 24, 597, 813, 949
BseGI GGATG 6 cut(s) 190, 464, 479, 526, 709, 1259
BseLI CCNNNNNNNGG 2 cut(s) 185, 474
BseMII CTCAG 3 cut(s) 194, 576, 793
BseNI ACTGG 2 cut(s) 337, 1141
BseRI GAGGAG 1 cut(s) 943
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 1 cut(s) 235
BseYI CCCAGC 1 cut(s) 1124
BshFI GGCC 1 cut(s) 844
BshNI GGYRCC 1 cut(s) 783
BshTI ACCGGT 1 cut(s) 292
BsiHKAI GWGCWC 2 cut(s) 490, 828
BsiSI CCGG 1 cut(s) 293
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 2 cut(s) 185, 474
BsmAI GTCTC 5 cut(s) 150, 283, 309, 511, 782
BsmBI CGTCTC 1 cut(s) 782
BsmFI GGGAC 1 cut(s) 371
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 1 cut(s) 844
Bso31I GGTCTC 1 cut(s) 283
Bsp1286I GDGCHC 2 cut(s) 490, 828
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 7 cut(s) 62, 188, 340, 620, 879, 1045, 1120
Bsp19I CCATGG 1 cut(s) 813
BspACI CCGC 4 cut(s) 44, 226, 336, 542
BspANI GGCC 1 cut(s) 844
BspCNI CTCAG 3 cut(s) 193, 575, 792
BspLI GGNNCC 4 cut(s) 64, 128, 190, 785
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 6 cut(s) 57, 70, 183, 196, 335, 1115
BspT107I GGYRCC 1 cut(s) 783
BspTNI GGTCTC 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 292
BsrGI TGTACA 1 cut(s) 367
BsrI ACTGG 2 cut(s) 337, 1141
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 4 cut(s) 24, 597, 813, 949
BssMI GATC 7 cut(s) 62, 188, 340, 620, 879, 1045, 1120
BssNI GRCGYC 1 cut(s) 784
BssT1I CCWWGG 3 cut(s) 24, 813, 949
Bst2UI CCWGG 2 cut(s) 599, 984
Bst4CI ACNGT 8 cut(s) 134, 205, 402, 453, 768, 963, 1204, 1250
Bst6I CTCTTC 3 cut(s) 412, 615, 1149
BstACI GRCGYC 1 cut(s) 784
BstAPI GCANNNNNTGC 1 cut(s) 662
BstAUI TGTACA 1 cut(s) 367
BstC8I GCNNGC 1 cut(s) 842
BstDEI CTNAG 3 cut(s) 180, 562, 779
BstDSI CCRYGG 1 cut(s) 813
BstF5I GGATG 6 cut(s) 190, 464, 479, 526, 709, 1259
BstH2I RGCGCY 1 cut(s) 787
BstHHI GCGC 1 cut(s) 786
BstKTI GATC 7 cut(s) 65, 191, 343, 623, 882, 1048, 1123
BstMAI GTCTC 5 cut(s) 150, 283, 309, 511, 782
BstMBI GATC 7 cut(s) 62, 188, 340, 620, 879, 1045, 1120
BstMWI GCNNNNNNNGC 2 cut(s) 206, 662
BstNI CCWGG 2 cut(s) 599, 984
BstSCI CCNGG 2 cut(s) 597, 982
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 490
BstV1I GCAGC 1 cut(s) 235
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 2 cut(s) 62, 188
BstXI CCANNNNNNTGG 1 cut(s) 1222
BstYI RGATCY 2 cut(s) 62, 188
Bsu36I CCTNAGG 1 cut(s) 180
BsuI GTATCC 1 cut(s) 489
BsuRI GGCC 1 cut(s) 844
BtgI CCRYGG 1 cut(s) 813
BtsCI GGATG 6 cut(s) 190, 464, 479, 526, 709, 1259
BtsIMutI CAGTG 3 cut(s) 130, 1080, 1175
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 842
CfoI GCGC 1 cut(s) 786
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 127, 1139
Csp6I GTAC 4 cut(s) 330, 368, 454, 946
CspAI ACCGGT 1 cut(s) 292
CviAII CATG 4 cut(s) 174, 274, 752, 814
CviQI GTAC 4 cut(s) 330, 368, 454, 946
DdeI CTNAG 3 cut(s) 180, 562, 779
DinI GGCGCC 1 cut(s) 785
DpnI GATC 7 cut(s) 64, 190, 342, 622, 881, 1047, 1122
DpnII GATC 7 cut(s) 62, 188, 340, 620, 879, 1045, 1120
DraI TTTAAA 1 cut(s) 262
Eam1104I CTCTTC 3 cut(s) 412, 615, 1149
EarI CTCTTC 3 cut(s) 412, 615, 1149
Ecl136II GAGCTC 1 cut(s) 826
Eco130I CCWWGG 3 cut(s) 24, 813, 949
Eco147I AGGCCT 1 cut(s) 844
Eco24I GRGCYC 1 cut(s) 828
Eco31I GGTCTC 1 cut(s) 283
Eco32I GATATC 1 cut(s) 569
Eco47I GGWCC 2 cut(s) 127, 1139
Eco53kI GAGCTC 1 cut(s) 826
Eco57I CTGAAG 1 cut(s) 593
Eco81I CCTNAGG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 826
EcoRII CCWGG 2 cut(s) 597, 982
EcoRV GATATC 1 cut(s) 569
EcoT14I CCWWGG 3 cut(s) 24, 813, 949
EcoT38I GRGCYC 1 cut(s) 828
EgeI GGCGCC 1 cut(s) 785
EheI GGCGCC 1 cut(s) 785
ErhI CCWWGG 3 cut(s) 24, 813, 949
Esp3I CGTCTC 1 cut(s) 782
FaeI CATG 4 cut(s) 177, 277, 755, 817
FalI AAGNNNNNCTT 2 cut(s) 475, 507
FaqI GGGAC 1 cut(s) 371
FatI CATG 4 cut(s) 173, 273, 751, 813
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 45, 224
FokI GGATG 6 cut(s) 197, 451, 486, 533, 716, 1266
FriOI GRGCYC 1 cut(s) 828
Fsp4HI GCNGC 2 cut(s) 45, 224
FspBI CTAG 7 cut(s) 354, 618, 639, 762, 950, 1146, 1187
GlaI GCGC 1 cut(s) 785
GluI GCNGC 2 cut(s) 45, 224
GsaI CCCAGC 1 cut(s) 1128
GsuI CTGGAG 1 cut(s) 570
HaeII RGCGCY 1 cut(s) 787
HaeIII GGCC 1 cut(s) 844
HapII CCGG 1 cut(s) 293
HhaI GCGC 1 cut(s) 786
Hin1I GRCGYC 1 cut(s) 784
Hin1II CATG 4 cut(s) 177, 277, 755, 817
Hin6I GCGC 1 cut(s) 784
HinP1I GCGC 1 cut(s) 784
HinfI GANTC 6 cut(s) 581, 719, 736, 748, 1102, 1180
HpaII CCGG 1 cut(s) 293
HphI GGTGA 5 cut(s) 350, 441, 613, 714, 1049
Hpy166II GTNNAC 5 cut(s) 31, 130, 161, 488, 907
Hpy188I TCNGA 5 cut(s) 83, 96, 283, 573, 1120
Hpy188III TCNNGA 8 cut(s) 182, 344, 564, 587, 740, 1049, 1146, 1242
Hpy8I GTNNAC 5 cut(s) 31, 130, 161, 488, 907
HpyAV CCTTC 2 cut(s) 1006, 1152
HpyCH4III ACNGT 8 cut(s) 134, 205, 402, 453, 768, 963, 1204, 1250
HpyCH4V TGCA 4 cut(s) 383, 488, 968, 1088
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 662
HpyF3I CTNAG 3 cut(s) 180, 562, 779
Hsp92I GRCGYC 1 cut(s) 784
Hsp92II CATG 4 cut(s) 177, 277, 755, 817
HspAI GCGC 1 cut(s) 784
KasI GGCGCC 1 cut(s) 783
Kzo9I GATC 7 cut(s) 62, 188, 340, 620, 879, 1045, 1120
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 4 cut(s) 594, 799, 928, 1075
MaeI CTAG 7 cut(s) 354, 618, 639, 762, 950, 1146, 1187
MaeIII GTNAC 3 cut(s) 720, 802, 1198
MalI GATC 7 cut(s) 64, 190, 342, 622, 881, 1047, 1122
MboI GATC 7 cut(s) 62, 188, 340, 620, 879, 1045, 1120
MboII GAAGA 5 cut(s) 119, 429, 602, 602, 1166
MflI RGATCY 2 cut(s) 62, 188
MhlI GDGCHC 2 cut(s) 490, 828
MluCI AATT 5 cut(s) 4, 249, 347, 867, 1149
Mly113I GGCGCC 1 cut(s) 784
MlyI GAGTC 3 cut(s) 728, 757, 1174
MmeI TCCRAC 2 cut(s) 1140, 1203
MnlI CCTC 9 cut(s) 76, 146, 189, 413, 511, 618, 921, 924, 1050
MroXI GAANNNNTTC 1 cut(s) 1167
MseI TTAA 3 cut(s) 261, 432, 441
MslI CAYNNNNRTG 1 cut(s) 96
MspA1I CMGCKG 1 cut(s) 226
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 2 cut(s) 599, 984
Mva1269I GAATGC 1 cut(s) 103
MvaI CCWGG 2 cut(s) 599, 984
MwoI GCNNNNNNNGC 2 cut(s) 206, 662
NarI GGCGCC 1 cut(s) 784
NcoI CCATGG 1 cut(s) 813
NdeII GATC 7 cut(s) 62, 188, 340, 620, 879, 1045, 1120
NlaIII CATG 4 cut(s) 177, 277, 755, 817
NlaIV GGNNCC 4 cut(s) 64, 128, 190, 785
NmuCI GTSAC 1 cut(s) 720
PceI AGGCCT 1 cut(s) 844
PctI GAATGC 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 1167
PfeI GAWTC 3 cut(s) 581, 736, 1102
PinAI ACCGGT 1 cut(s) 292
PkrI GCNGC 2 cut(s) 46, 225
PleI GAGTC 3 cut(s) 727, 756, 1174
PluTI GGCGCC 1 cut(s) 787
PpsI GAGTC 3 cut(s) 727, 756, 1174
Psp124BI GAGCTC 1 cut(s) 828
Psp6I CCWGG 2 cut(s) 597, 982
PspFI CCCAGC 1 cut(s) 1124
PspGI CCWGG 2 cut(s) 597, 982
PspN4I GGNNCC 4 cut(s) 64, 128, 190, 785
PspPI GGNCC 2 cut(s) 127, 1139
PsuI RGATCY 2 cut(s) 62, 188
RsaI GTAC 4 cut(s) 331, 369, 455, 947
RsaNI GTAC 4 cut(s) 330, 368, 454, 946
RseI CAYNNNNRTG 1 cut(s) 96
SacI GAGCTC 1 cut(s) 828
SaqAI TTAA 3 cut(s) 261, 432, 441
SatI GCNGC 2 cut(s) 45, 224
Sau3AI GATC 7 cut(s) 62, 188, 340, 620, 879, 1045, 1120
Sau96I GGNCC 2 cut(s) 127, 1139
SchI GAGTC 3 cut(s) 728, 757, 1174
ScrFI CCNGG 2 cut(s) 599, 984
SduI GDGCHC 2 cut(s) 490, 828
SfaNI GCATC 4 cut(s) 594, 799, 928, 1075
SfcI CTRYAG 1 cut(s) 204
SfoI GGCGCC 1 cut(s) 785
SinI GGWCC 2 cut(s) 127, 1139
SmiMI CAYNNNNRTG 1 cut(s) 96
Sse9I AATT 5 cut(s) 4, 249, 347, 867, 1149
SseBI AGGCCT 1 cut(s) 844
SsiI CCGC 4 cut(s) 44, 226, 336, 542
SspDI GGCGCC 1 cut(s) 783
SspMI CTAG 7 cut(s) 354, 618, 639, 762, 950, 1146, 1187
SstI GAGCTC 1 cut(s) 828
StuI AGGCCT 1 cut(s) 844
StyD4I CCNGG 2 cut(s) 597, 982
StyI CCWWGG 3 cut(s) 24, 813, 949
TaaI ACNGT 8 cut(s) 134, 205, 402, 453, 768, 963, 1204, 1250
TaqI TCGA 1 cut(s) 1171
TasI AATT 5 cut(s) 4, 249, 347, 867, 1149
TatI WGTACW 1 cut(s) 367
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 3 cut(s) 581, 736, 1102
Tru1I TTAA 3 cut(s) 261, 432, 441
Tru9I TTAA 3 cut(s) 261, 432, 441
TscAI CASTG 3 cut(s) 137, 1087, 1182
TseFI GTSAC 1 cut(s) 720
TseI GCWGC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 720
TspDTI ATGAA 5 cut(s) 17, 162, 453, 537, 1287
TspRI CASTG 3 cut(s) 137, 1087, 1182
VneI GTGCAC 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 127, 1139
XbaI TCTAGA 1 cut(s) 1145
XmaJI CCTAGG 1 cut(s) 949
XmiI GTMKAC 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 1167
XspI CTAG 7 cut(s) 354, 618, 639, 762, 950, 1146, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.