Rw1G040390

Belongs to the phospholipid scramblase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0017071
Physical Location & Seq
Forward (+)
67690176 .. 67694021
3846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G040390.1

Sequence Viewer

Length: 708 bp
ATGAATTGGAGAAGTAGTTGGCTTAGCCTCACTAAGGTTTACAAAGCTGCGGCAGCAGATTCCAAATGTGGCTCTTCCTTTTGGTCAGGGCTAGCTTTGTCTCGGAAATTTGGAGACAAGGCTAAGGCTGATACTGGGTTTGACCTGAATAGGGACTTCCTTGTACAGCTATGGATGGCGGATAAGGAAATGGAGCAGTTTCGGAGAAAATCGAGACGGAGGGCTGTTGAGCAAGATGAGAATAGCGTGGTTGGGAAGCAACCACCTGTGAGCCAATCCATCTCGGGATATCTGAAGCCGGAATCTCCAGAAGAGGTCCAGGTGATGCCTCTTCTTGCTAGATCAAATATGCTCGTTACTAGGGATATAGAGTGGGCAAATCTTATGCTTGGGTTTGAGCAGGAAAATCGGTATGCCATAGTAGATGTATGCTATCCACAGTCGCCTGTAGGTTTCATTCGTGAGGAGAGTCATGTTATCATGAGACAGTTACTTCGCCAGCGGCGTCCTTTTGTTGCTCGAGTAACTGATGGCATGGGAAATGAGCTCTTTAGGGTTCGCAGGCCTTTTTGGTGGATAAACAGCTCAATTTATGCAGAGATCAATGGTAAGGAAGTTGGTGTGGTTCACAGACGATGGCATTTGTGGAGAAGGATTTATGATTTGTACCTAGGGTACTTTCCAACAGCTTATTTCCCCTGTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Scramblase

Protein Analysis

235

Amino Acids

27.54

Weight (kDa)

9.58

Isoelectric Point (pI)

69.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Scramblase PF03803 100 - 224 6.2e-36 Scramblase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04940
fragaria_vesca FvH4_7g33592
malus_domestica MD01G1233700.v1.1 MD07G1305800.v1.1
prunus_persica Prupe.2G323900_v2.0.a1
pyrus_communis pycom01g24200
rosa_chinensis RchiOBHm_Chr1g0383851
rosa_laevigata RLG00000026091
rosa_samantha Rh1AG468500 Rh1BG426200 Rh1CG438900 Rh1DG459000
rosa_wichuraiana Rw1G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 50, 179, 502
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 505
AfaI GTAC 3 cut(s) 165, 668, 677
AfiI CCNNNNNNNGG 2 cut(s) 34, 151
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 7 cut(s) 47, 95, 169, 547, 585, 689, 705
AluI AGCT 7 cut(s) 47, 95, 169, 547, 585, 689, 705
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 4 cut(s) 105, 108, 208, 478
Ama87I CYCGRG 2 cut(s) 283, 519
AoxI GGCC 1 cut(s) 563
ApeKI GCWGC 2 cut(s) 47, 53
ApoI RAATTY 1 cut(s) 107
ArsI GACNNNNNNTTYG 2 cut(s) 477, 509
AspA2I CCTAGG 1 cut(s) 670
AspS9I GGNCC 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 334
AsuNHI GCTAGC 1 cut(s) 91
AvaI CYCGRG 2 cut(s) 283, 519
AvaII GGWCC 1 cut(s) 316
AvrII CCTAGG 1 cut(s) 670
BanII GRGCYC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 2 cut(s) 34, 65
BccI CCATC 4 cut(s) 169, 287, 524, 630
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 1 cut(s) 320
BcoDI GTCTC 4 cut(s) 105, 108, 208, 478
BfaI CTAG 4 cut(s) 92, 339, 360, 671
BfmI CTRYAG 2 cut(s) 447, 700
BisI GCNGC 4 cut(s) 48, 51, 54, 503
BlnI CCTAGG 1 cut(s) 670
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 4 cut(s) 49, 52, 55, 504
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 2 cut(s) 283, 519
BmgT120I GGNCC 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 320
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 315
BmtI GCTAGC 1 cut(s) 95
BmuI ACTGGG 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 123
Bpu1102I GCTNAGC 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 670
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 151
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 670
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 34, 151
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 1 cut(s) 479
BseXI GCAGC 2 cut(s) 34, 65
BshFI GGCC 1 cut(s) 565
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 2 cut(s) 283, 519
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 2 cut(s) 34, 151
BsmAI GTCTC 4 cut(s) 105, 108, 208, 478
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 1 cut(s) 565
BsoBI CYCGRG 2 cut(s) 283, 519
Bsp1286I GDGCHC 1 cut(s) 549
Bsp1407I TGTACA 1 cut(s) 163
Bsp143I GATC 2 cut(s) 341, 600
Bsp1720I GCTNAGC 1 cut(s) 23
BspACI CCGC 3 cut(s) 50, 179, 502
BspANI GGCC 1 cut(s) 565
BspHI TCATGA 1 cut(s) 480
BspOI GCTAGC 1 cut(s) 95
BspQI GCTCTTC 1 cut(s) 79
BsrGI TGTACA 1 cut(s) 163
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 670
BssMI GATC 2 cut(s) 341, 600
BssNI GRCGYC 1 cut(s) 505
BssT1I CCWWGG 1 cut(s) 670
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 2 cut(s) 441, 489
Bst6I CTCTTC 3 cut(s) 79, 306, 336
BstACI GRCGYC 1 cut(s) 505
BstAUI TGTACA 1 cut(s) 163
BstC8I GCNNGC 3 cut(s) 93, 500, 563
BstDEI CTNAG 3 cut(s) 23, 33, 123
BstENI CCTNNNNNAGG 1 cut(s) 32
BstF5I GGATG 1 cut(s) 180
BstKTI GATC 2 cut(s) 344, 603
BstMAI GTCTC 4 cut(s) 105, 108, 208, 478
BstMBI GATC 2 cut(s) 341, 600
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 2 cut(s) 447, 700
BstV1I GCAGC 2 cut(s) 34, 65
BsuRI GGCC 1 cut(s) 565
BtsCI GGATG 1 cut(s) 180
Cac8I GCNNGC 3 cut(s) 93, 500, 563
CciI TCATGA 1 cut(s) 480
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 1 cut(s) 494
Csp6I GTAC 3 cut(s) 164, 667, 676
CviAII CATG 3 cut(s) 473, 481, 535
CviQI GTAC 3 cut(s) 164, 667, 676
DdeI CTNAG 3 cut(s) 23, 33, 123
DpnI GATC 2 cut(s) 343, 602
DpnII GATC 2 cut(s) 341, 600
Eam1104I CTCTTC 3 cut(s) 79, 306, 336
EarI CTCTTC 3 cut(s) 79, 306, 336
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 670
Eco147I AGGCCT 1 cut(s) 565
Eco24I GRGCYC 1 cut(s) 549
Eco32I GATATC 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 2 cut(s) 283, 519
EcoICRI GAGCTC 1 cut(s) 547
EcoNI CCTNNNNNAGG 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 318
EcoRV GATATC 1 cut(s) 290
EcoT14I CCWWGG 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 670
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 3 cut(s) 476, 484, 538
FaqI GGGAC 1 cut(s) 167
FatI CATG 3 cut(s) 472, 480, 534
Fnu4HI GCNGC 4 cut(s) 48, 51, 54, 503
FokI GGATG 1 cut(s) 187
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 4 cut(s) 48, 51, 54, 503
FspBI CTAG 4 cut(s) 92, 339, 360, 671
GluI GCNGC 4 cut(s) 48, 51, 54, 503
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 1 cut(s) 565
HapII CCGG 1 cut(s) 299
HgaI GACGC 1 cut(s) 494
Hin1I GRCGYC 1 cut(s) 505
Hin1II CATG 3 cut(s) 476, 484, 538
HinfI GANTC 3 cut(s) 59, 302, 469
HpaII CCGG 1 cut(s) 299
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 2 cut(s) 40, 628
Hpy188I TCNGA 3 cut(s) 105, 204, 294
Hpy188III TCNNGA 5 cut(s) 213, 285, 308, 461, 481
Hpy8I GTNNAC 2 cut(s) 40, 628
HpyAV CCTTC 1 cut(s) 645
HpyCH4III ACNGT 2 cut(s) 441, 489
HpyCH4V TGCA 1 cut(s) 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 3 cut(s) 23, 33, 123
Hsp92I GRCGYC 1 cut(s) 505
Hsp92II CATG 3 cut(s) 476, 484, 538
Kzo9I GATC 2 cut(s) 341, 600
LguI GCTCTTC 1 cut(s) 79
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 2 cut(s) 34, 65
LweI GCATC 1 cut(s) 315
MaeI CTAG 4 cut(s) 92, 339, 360, 671
MaeIII GTNAC 3 cut(s) 355, 489, 523
MalI GATC 2 cut(s) 343, 602
MboI GATC 2 cut(s) 341, 600
MboII GAAGA 3 cut(s) 66, 323, 323
MhlI GDGCHC 1 cut(s) 549
MluCI AATT 3 cut(s) 4, 107, 588
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 1 cut(s) 707
MnlI CCTC 5 cut(s) 38, 213, 307, 339, 457
MspA1I CMGCKG 1 cut(s) 502
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 1 cut(s) 53
NdeII GATC 2 cut(s) 341, 600
NheI GCTAGC 1 cut(s) 91
NlaIII CATG 3 cut(s) 476, 484, 538
PaeR7I CTCGAG 1 cut(s) 519
PagI TCATGA 1 cut(s) 480
PceI AGGCCT 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 502
PfeI GAWTC 2 cut(s) 59, 302
PkrI GCNGC 4 cut(s) 49, 52, 55, 504
PleI GAGTC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 549
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspPI GGNCC 1 cut(s) 316
PspXI VCTCGAGB 1 cut(s) 519
RsaI GTAC 3 cut(s) 165, 668, 677
RsaNI GTAC 3 cut(s) 164, 667, 676
SacI GAGCTC 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 79
SatI GCNGC 4 cut(s) 48, 51, 54, 503
Sau3AI GATC 2 cut(s) 341, 600
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 1 cut(s) 549
SfaNI GCATC 1 cut(s) 315
SfcI CTRYAG 2 cut(s) 447, 700
Sfr274I CTCGAG 1 cut(s) 519
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 519
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
Sse9I AATT 3 cut(s) 4, 107, 588
SseBI AGGCCT 1 cut(s) 565
SsiI CCGC 3 cut(s) 50, 179, 502
SspMI CTAG 4 cut(s) 92, 339, 360, 671
SstI GAGCTC 1 cut(s) 549
StuI AGGCCT 1 cut(s) 565
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 1 cut(s) 670
TaaI ACNGT 2 cut(s) 441, 489
TaqI TCGA 2 cut(s) 212, 520
TasI AATT 3 cut(s) 4, 107, 588
TatI WGTACW 1 cut(s) 163
TauI GCSGC 2 cut(s) 53, 505
TfiI GAWTC 2 cut(s) 59, 302
TseI GCWGC 2 cut(s) 47, 53
TspDTI ATGAA 2 cut(s) 17, 445
TspGWI ACGGA 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 316
XagI CCTNNNNNAGG 1 cut(s) 32
XapI RAATTY 1 cut(s) 107
XhoI CTCGAG 1 cut(s) 519
XmaJI CCTAGG 1 cut(s) 670
XspI CTAG 4 cut(s) 92, 339, 360, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.