RLG00000026091

Belongs to the phospholipid scramblase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017071
Physical Location & Seq
Forward (+)
493903 .. 497552
3650 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026091

Sequence Viewer

Length: 1071 bp
ATGCATAGTGAGATTCTTGAAAAGGGTAGAGAACCGCCTGAGCTCAACTCAACTGAACCGATGAATTGGAGAAGTAGTTGGCTTTGCCTCACTAAGGTTTACAAAGCCGCGGCAGCAGATTCCAAATGTGGCTCTTCCTTGTGGTCAGGGCTAGCTTTGTCTCGGAAATTTGGAGACAAGGCTAAGGCTGATACTGGGTTTGAGCTGAATAGGGACTTCTTTGTACAGCTATGGATGGCTGATAAGGAAATGGAGCAGTTTCAGAGAAAATCGAGACGGAGGGCTGTTGAGCAAGATGAGAATAGCGTGGTTGGGAAGCAACCACCTGTGAGCCAATCCATCTCGGGATATCTGAAGCCGGAATCTCCAGAAGAGGTCCAGGTGATGCCTCTTCTTGCTAGATCAAATATGCTCGTTACTAGGGATATAGAGTGGGCAAATCTTATGCTTGGGTTTGAGCAGGAAAATCGGTATGCCATAGTAGATGTATGCTATCCACAATCGCCTGTAGGTTTCATTCGTGAGGAGAGTCATGTTATCATGAGACAGTTACTTCGCCAGCGGCGCCCTTTTGTTGCTCGAGTAACTGATGGCATGGGTAATGAGCTCTTTAGGGTTCGCAGGCCCTTTTGGTGGATAAACAGCTCAATTTATGCAGAGATCAATGGTAAGGAAGTTGGTGTGGTTCACAGACGATGGCATTTGTGGAGAAGGATATATGATTTGTACCTAGGGAATAAACAATTTGCAGTTGTTGAAAATCCTGGATTCTGGCATTGGACATTTACTTTGAAGGACATTGATGGGGGTGTTTTGGCTGAGATAGATCGTGATTGGAGGGGCTTTGGCTTTGAGCTGTTTACTGATGCTGGGCAATATGTGATTCGGTTTGGGAGCTCTGATCCCAGCTCCAAGATAGGCCTTGCTAGCCAGATTGAAGAGTTGGAAGTAGTTCGCCCGTTGACTCTGTCAGAGAGAGCTGTAACTCTTGCTCTTGCTATATCGTTGGATAATGACTATTTTTCAAGACACGGTGGATGGGGAATACCTTATTTTGATGTAGGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Scramblase

Protein Analysis

357

Amino Acids

41.08

Weight (kDa)

6.16

Isoelectric Point (pI)

60.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Scramblase PF03803 120 - 343 3.8e-63 Scramblase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04940
fragaria_vesca FvH4_7g33592
malus_domestica MD01G1233700.v1.1 MD07G1305800.v1.1
prunus_persica Prupe.2G323900_v2.0.a1
pyrus_communis pycom01g24200
rosa_chinensis RchiOBHm_Chr1g0383851
rosa_laevigata RLG00000026091
rosa_samantha Rh1AG468500 Rh1BG426200 Rh1CG438900 Rh1DG459000
rosa_wichuraiana Rw1G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 564
AccII CGCG 1 cut(s) 110
AciI CCGC 4 cut(s) 35, 108, 110, 562
AclWI GGATC 1 cut(s) 896
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 565
AfaI GTAC 2 cut(s) 225, 728
AfiI CCNNNNNNNGG 2 cut(s) 94, 633
AgsI TTSAA 5 cut(s) 20, 758, 793, 938, 1026
AjnI CCWGG 2 cut(s) 378, 763
Alw21I GWGCWC 3 cut(s) 45, 609, 899
Alw26I GTCTC 4 cut(s) 165, 168, 268, 538
AlwI GGATC 1 cut(s) 896
Ama87I CYCGRG 2 cut(s) 343, 579
AoxI GGCC 2 cut(s) 623, 919
ApeKI GCWGC 1 cut(s) 113
ApoI RAATTY 1 cut(s) 167
ArsI GACNNNNNNTTYG 4 cut(s) 537, 569, 772, 804
Asp700I GAANNNNTTC 1 cut(s) 951
AspA2I CCTAGG 1 cut(s) 730
AspLEI GCGC 1 cut(s) 567
AspS9I GGNCC 2 cut(s) 376, 624
AsuHPI GGTGA 1 cut(s) 394
AsuNHI GCTAGC 2 cut(s) 151, 926
AvaI CYCGRG 2 cut(s) 343, 579
AvaII GGWCC 1 cut(s) 376
AvrII CCTAGG 1 cut(s) 730
BanI GGYRCC 1 cut(s) 564
BanII GRGCYC 3 cut(s) 45, 609, 899
Bbv12I GWGCWC 3 cut(s) 45, 609, 899
BbvI GCAGC 1 cut(s) 125
BccI CCATC 6 cut(s) 229, 347, 584, 690, 797, 1032
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 2 cut(s) 380, 765
BcoDI GTCTC 4 cut(s) 165, 168, 268, 538
BfaI CTAG 5 cut(s) 152, 399, 420, 731, 927
BfmI CTRYAG 1 cut(s) 507
BfoI RGCGCY 1 cut(s) 568
BisI GCNGC 4 cut(s) 108, 111, 114, 563
BlnI CCTAGG 1 cut(s) 730
BlsI GCNGC 4 cut(s) 109, 112, 115, 564
Bme1390I CCNGG 2 cut(s) 380, 765
Bme18I GGWCC 1 cut(s) 376
BmeT110I CYCGRG 2 cut(s) 343, 579
BmgT120I GGNCC 2 cut(s) 376, 624
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 2 cut(s) 380, 765
BmrI ACTGGG 1 cut(s) 204
BmsI GCATC 2 cut(s) 375, 856
BmtI GCTAGC 2 cut(s) 155, 930
BmuI ACTGGG 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 32, 64
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 2 cut(s) 39, 183
BsaHI GRCGYC 1 cut(s) 565
BsaJI CCNNGG 2 cut(s) 108, 730
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 633
Bse1I ACTGG 1 cut(s) 199
BseBI CCWGG 2 cut(s) 380, 765
BseDI CCNNGG 2 cut(s) 108, 730
BseGI GGATG 2 cut(s) 240, 1043
BseLI CCNNNNNNNGG 2 cut(s) 94, 633
BseMII CTCAG 2 cut(s) 30, 810
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 1 cut(s) 125
BseYI CCCAGC 2 cut(s) 869, 905
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 2 cut(s) 625, 921
BshNI GGYRCC 1 cut(s) 564
BsiHKAI GWGCWC 3 cut(s) 45, 609, 899
BsiHKCI CYCGRG 2 cut(s) 343, 579
BsiSI CCGG 1 cut(s) 359
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 2 cut(s) 94, 633
BsmAI GTCTC 4 cut(s) 165, 168, 268, 538
BsmBI CGTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 227
BsnI GGCC 2 cut(s) 625, 921
BsoBI CYCGRG 2 cut(s) 343, 579
Bsp1286I GDGCHC 3 cut(s) 45, 609, 899
Bsp1407I TGTACA 1 cut(s) 223
Bsp143I GATC 4 cut(s) 401, 660, 826, 901
BspACI CCGC 4 cut(s) 35, 108, 110, 562
BspANI GGCC 2 cut(s) 625, 921
BspCNI CTCAG 2 cut(s) 31, 811
BspFNI CGCG 1 cut(s) 110
BspHI TCATGA 1 cut(s) 540
BspLI GGNNCC 1 cut(s) 566
BspOI GCTAGC 2 cut(s) 155, 930
BspPI GGATC 1 cut(s) 896
BspQI GCTCTTC 1 cut(s) 139
BspT107I GGYRCC 1 cut(s) 564
BsrGI TGTACA 1 cut(s) 223
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 2 cut(s) 108, 730
BssMI GATC 4 cut(s) 401, 660, 826, 901
BssNI GRCGYC 1 cut(s) 565
BssT1I CCWWGG 1 cut(s) 730
Bst2UI CCWGG 2 cut(s) 380, 765
Bst4CI ACNGT 2 cut(s) 549, 1034
Bst6I CTCTTC 4 cut(s) 139, 366, 396, 933
BstACI GRCGYC 1 cut(s) 565
BstAUI TGTACA 1 cut(s) 223
BstC8I GCNNGC 4 cut(s) 153, 560, 623, 928
BstDEI CTNAG 4 cut(s) 39, 93, 183, 819
BstDSI CCRYGG 1 cut(s) 108
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 2 cut(s) 240, 1043
BstFNI CGCG 1 cut(s) 110
BstH2I RGCGCY 1 cut(s) 568
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 4 cut(s) 404, 663, 829, 904
BstMAI GTCTC 4 cut(s) 165, 168, 268, 538
BstMBI GATC 4 cut(s) 401, 660, 826, 901
BstMWI GCNNNNNNNGC 3 cut(s) 113, 564, 927
BstNI CCWGG 2 cut(s) 380, 765
BstSCI CCNGG 2 cut(s) 378, 763
BstSFI CTRYAG 1 cut(s) 507
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 1 cut(s) 125
BsuRI GGCC 2 cut(s) 625, 921
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 2 cut(s) 240, 1043
Cac8I GCNNGC 4 cut(s) 153, 560, 623, 928
CciI TCATGA 1 cut(s) 540
CfoI GCGC 1 cut(s) 567
Cfr13I GGNCC 2 cut(s) 376, 624
Cfr42I CCGCGG 1 cut(s) 111
Csp6I GTAC 2 cut(s) 224, 727
CviAII CATG 3 cut(s) 533, 541, 595
CviQI GTAC 2 cut(s) 224, 727
DdeI CTNAG 4 cut(s) 39, 93, 183, 819
DinI GGCGCC 1 cut(s) 566
DpnI GATC 4 cut(s) 403, 662, 828, 903
DpnII GATC 4 cut(s) 401, 660, 826, 901
Eam1104I CTCTTC 4 cut(s) 139, 366, 396, 933
EarI CTCTTC 4 cut(s) 139, 366, 396, 933
Ecl136II GAGCTC 3 cut(s) 43, 607, 897
Eco130I CCWWGG 1 cut(s) 730
Eco147I AGGCCT 1 cut(s) 921
Eco24I GRGCYC 3 cut(s) 45, 609, 899
Eco32I GATATC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 376
Eco53kI GAGCTC 3 cut(s) 43, 607, 897
Eco57I CTGAAG 1 cut(s) 374
Eco88I CYCGRG 2 cut(s) 343, 579
EcoICRI GAGCTC 3 cut(s) 43, 607, 897
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 624
EcoRII CCWGG 2 cut(s) 378, 763
EcoRV GATATC 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 730
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 45, 609, 899
EgeI GGCGCC 1 cut(s) 566
EheI GGCGCC 1 cut(s) 566
ErhI CCWWGG 1 cut(s) 730
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 3 cut(s) 536, 544, 598
FaqI GGGAC 1 cut(s) 227
FatI CATG 3 cut(s) 532, 540, 594
Fnu4HI GCNGC 4 cut(s) 108, 111, 114, 563
FokI GGATG 2 cut(s) 247, 1050
FriOI GRGCYC 3 cut(s) 45, 609, 899
Fsp4HI GCNGC 4 cut(s) 108, 111, 114, 563
FspBI CTAG 5 cut(s) 152, 399, 420, 731, 927
GlaI GCGC 1 cut(s) 566
GluI GCNGC 4 cut(s) 108, 111, 114, 563
GsaI CCCAGC 2 cut(s) 873, 909
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 568
HaeIII GGCC 2 cut(s) 625, 921
HapII CCGG 1 cut(s) 359
HhaI GCGC 1 cut(s) 567
Hin1I GRCGYC 1 cut(s) 565
Hin1II CATG 3 cut(s) 536, 544, 598
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HincII GTYRAC 1 cut(s) 963
HindII GTYRAC 1 cut(s) 963
HinfI GANTC 7 cut(s) 13, 119, 362, 529, 768, 883, 964
HpaII CCGG 1 cut(s) 359
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 4 cut(s) 100, 688, 861, 963
Hpy188I TCNGA 5 cut(s) 165, 264, 354, 901, 973
Hpy188III TCNNGA 8 cut(s) 17, 273, 345, 368, 521, 541, 830, 1026
Hpy8I GTNNAC 4 cut(s) 100, 688, 861, 963
HpyAV CCTTC 2 cut(s) 705, 787
HpyCH4III ACNGT 2 cut(s) 549, 1034
HpyCH4V TGCA 3 cut(s) 4, 656, 749
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 564, 927
HpyF3I CTNAG 4 cut(s) 39, 93, 183, 819
Hsp92I GRCGYC 1 cut(s) 565
Hsp92II CATG 3 cut(s) 536, 544, 598
HspAI GCGC 1 cut(s) 565
KasI GGCGCC 1 cut(s) 564
KspI CCGCGG 1 cut(s) 111
Kzo9I GATC 4 cut(s) 401, 660, 826, 901
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 3 cut(s) 253, 894, 914
Lsp1109I GCAGC 1 cut(s) 125
LweI GCATC 2 cut(s) 375, 856
MaeI CTAG 5 cut(s) 152, 399, 420, 731, 927
MaeIII GTNAC 4 cut(s) 415, 549, 583, 982
MalI GATC 4 cut(s) 403, 662, 828, 903
MboI GATC 4 cut(s) 401, 660, 826, 901
MboII GAAGA 4 cut(s) 126, 383, 383, 950
MhlI GDGCHC 3 cut(s) 45, 609, 899
MluCI AATT 4 cut(s) 64, 167, 648, 743
Mly113I GGCGCC 1 cut(s) 565
MlyI GAGTC 2 cut(s) 538, 958
MmeI TCCRAC 2 cut(s) 924, 987
MnlI CCTC 6 cut(s) 98, 273, 367, 399, 517, 831
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 951
MspA1I CMGCKG 2 cut(s) 110, 562
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 2 cut(s) 380, 765
MvaI CCWGG 2 cut(s) 380, 765
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 3 cut(s) 113, 564, 927
NarI GGCGCC 1 cut(s) 565
NdeII GATC 4 cut(s) 401, 660, 826, 901
NheI GCTAGC 2 cut(s) 151, 926
NlaIII CATG 3 cut(s) 536, 544, 598
NlaIV GGNNCC 1 cut(s) 566
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 579
PagI TCATGA 1 cut(s) 540
PceI AGGCCT 1 cut(s) 921
PciSI GCTCTTC 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 951
PfeI GAWTC 5 cut(s) 13, 119, 362, 768, 883
PflFI GACNNNGTC 1 cut(s) 967
PfoI TCCNGGA 1 cut(s) 763
PkrI GCNGC 4 cut(s) 109, 112, 115, 564
PleI GAGTC 2 cut(s) 537, 958
PluTI GGCGCC 1 cut(s) 568
PpsI GAGTC 2 cut(s) 537, 958
Psp124BI GAGCTC 3 cut(s) 45, 609, 899
Psp6I CCWGG 2 cut(s) 378, 763
PspFI CCCAGC 2 cut(s) 869, 905
PspGI CCWGG 2 cut(s) 378, 763
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 2 cut(s) 376, 624
PspXI VCTCGAGB 1 cut(s) 579
PsyI GACNNNGTC 1 cut(s) 967
RsaI GTAC 2 cut(s) 225, 728
RsaNI GTAC 2 cut(s) 224, 727
SacI GAGCTC 3 cut(s) 45, 609, 899
SacII CCGCGG 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 139
SatI GCNGC 4 cut(s) 108, 111, 114, 563
Sau3AI GATC 4 cut(s) 401, 660, 826, 901
Sau96I GGNCC 2 cut(s) 376, 624
SchI GAGTC 2 cut(s) 538, 958
ScrFI CCNGG 2 cut(s) 380, 765
SduI GDGCHC 3 cut(s) 45, 609, 899
SfaNI GCATC 2 cut(s) 375, 856
SfcI CTRYAG 1 cut(s) 507
SfoI GGCGCC 1 cut(s) 566
Sfr274I CTCGAG 1 cut(s) 579
Sfr303I CCGCGG 1 cut(s) 111
SgrBI CCGCGG 1 cut(s) 111
SinI GGWCC 1 cut(s) 376
SlaI CTCGAG 1 cut(s) 579
SmlI CTYRAG 1 cut(s) 579
SmoI CTYRAG 1 cut(s) 579
Sse9I AATT 4 cut(s) 64, 167, 648, 743
SseBI AGGCCT 1 cut(s) 921
SsiI CCGC 4 cut(s) 35, 108, 110, 562
SspDI GGCGCC 1 cut(s) 564
SspMI CTAG 5 cut(s) 152, 399, 420, 731, 927
SstI GAGCTC 3 cut(s) 45, 609, 899
StuI AGGCCT 1 cut(s) 921
StyD4I CCNGG 2 cut(s) 378, 763
StyI CCWWGG 1 cut(s) 730
TaaI ACNGT 2 cut(s) 549, 1034
TaqI TCGA 2 cut(s) 272, 580
TasI AATT 4 cut(s) 64, 167, 648, 743
TatI WGTACW 1 cut(s) 223
TauI GCSGC 3 cut(s) 110, 113, 565
TfiI GAWTC 5 cut(s) 13, 119, 362, 768, 883
TseI GCWGC 1 cut(s) 113
TspDTI ATGAA 2 cut(s) 77, 505
TspGWI ACGGA 1 cut(s) 292
Tth111I GACNNNGTC 1 cut(s) 967
VpaK11BI GGWCC 1 cut(s) 376
XagI CCTNNNNNAGG 1 cut(s) 92
XapI RAATTY 1 cut(s) 167
XhoI CTCGAG 1 cut(s) 579
XmaJI CCTAGG 1 cut(s) 730
XmnI GAANNNNTTC 1 cut(s) 951
XspI CTAG 5 cut(s) 152, 399, 420, 731, 927
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.