Rh1BG426200

Belongs to the phospholipid scramblase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0017071
Physical Location & Seq
Reverse (-)
54414739 .. 54418374
3636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG426200.1

Sequence Viewer

Length: 861 bp
ATGAATTGGAGAAGTAGTTGGCTTAGCCTCACTAAGGTTTACAAAGCTGCGGCAGCAGATTCCAAATGTGGCTCTTCCTTGTGGTCAGGGCTAGCTTTGTCTCGGAAATTTGGAGACAAGGCTAAGGCTGATACTGGGTTTGACCTGAATAGGGACTTCCTTGTACAGCTATGGATGGCGGATAAGGAAATGGAGCAGTTTCGGAGAAAATCGAGACGGAGGGCTGTTGAGCAAGATGAGAATAGCGTGGTTGGGAAGCAACCACCTGTGAGCCAATCCATCTCGGGATATCTGAAGCCGGAATCTCCAGAAGAGGTCCAGGTGATGCCTCTTCTTGCTAGATCAAATATGCTCGTTACTAGGGATATAGAGTGGGCAAATCTTATGCTTGGGTTTGAGCAGGAAAATCGGTATGCCATAGTAGATGTATGCTATCCACAGTCGCCTGTAGGTTTCATTCGTGAGGAGAGTCATGTTATCATGAGACAGTTACTTCGCCAGCGGCGTCCTTTTGTTGCTCGAGTAACTGATGGCATGGGAAATGAGCTCTTTAGGGTTCGCAGGCCTTTTTGGTGGATAAACAGCTCAATTTATGCAGAGATCAATGGTAAGGAAGTTGGTGTGGTTCACAGACGATGGCATTTGTGGAGAAGGATTTATGATTTGTACCTAGGGAATAAACAATTTGCAGTTGTTGAAAATCCTGGATTCTGGCATTGGACATTTACTTTGAAGGACATTGATGGGGGTGTGTTGGCCGAGATAGATCGTGATTGGAGGGGTTTTGGCTTTGAGGTTGTTTCATTATCTAAATCTCTAAGATACTTGCATTCTGGTGCCTTCTCTTCATCCCACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Scramblase

Protein Analysis

286

Amino Acids

33.27

Weight (kDa)

9.51

Isoelectric Point (pI)

63.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Scramblase PF03803 100 - 267 8.3e-48 Scramblase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04940
fragaria_vesca FvH4_7g33592
malus_domestica MD01G1233700.v1.1 MD07G1305800.v1.1
prunus_persica Prupe.2G323900_v2.0.a1
pyrus_communis pycom01g24200
rosa_chinensis RchiOBHm_Chr1g0383851
rosa_laevigata RLG00000026091
rosa_samantha Rh1AG468500 Rh1BG426200 Rh1CG438900 Rh1DG459000
rosa_wichuraiana Rw1G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 836
AciI CCGC 3 cut(s) 50, 179, 502
AcoI YGGCCR 1 cut(s) 756
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 505
AfaI GTAC 2 cut(s) 165, 668
AfiI CCNNNNNNNGG 2 cut(s) 34, 151
AgsI TTSAA 2 cut(s) 698, 733
AjnI CCWGG 2 cut(s) 318, 703
AluBI AGCT 5 cut(s) 47, 95, 169, 547, 585
AluI AGCT 5 cut(s) 47, 95, 169, 547, 585
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 4 cut(s) 105, 108, 208, 478
Ama87I CYCGRG 2 cut(s) 283, 519
AoxI GGCC 2 cut(s) 563, 756
ApeKI GCWGC 2 cut(s) 47, 53
ApoI RAATTY 1 cut(s) 107
ArsI GACNNNNNNTTYG 4 cut(s) 477, 509, 712, 744
AspA2I CCTAGG 1 cut(s) 670
AspS9I GGNCC 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 334
AsuNHI GCTAGC 1 cut(s) 91
AvaI CYCGRG 2 cut(s) 283, 519
AvaII GGWCC 1 cut(s) 316
AvrII CCTAGG 1 cut(s) 670
BanI GGYRCC 1 cut(s) 836
BanII GRGCYC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 2 cut(s) 34, 65
BccI CCATC 5 cut(s) 169, 287, 524, 630, 737
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 2 cut(s) 320, 705
BcoDI GTCTC 4 cut(s) 105, 108, 208, 478
BfaI CTAG 4 cut(s) 92, 339, 360, 671
BfmI CTRYAG 1 cut(s) 447
BisI GCNGC 4 cut(s) 48, 51, 54, 503
BlnI CCTAGG 1 cut(s) 670
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 4 cut(s) 49, 52, 55, 504
Bme1390I CCNGG 2 cut(s) 320, 705
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 2 cut(s) 283, 519
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 838
BmrFI CCNGG 2 cut(s) 320, 705
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 315
BmtI GCTAGC 1 cut(s) 95
BmuI ACTGGG 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 123
Bpu1102I GCTNAGC 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 670
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 151
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 2 cut(s) 320, 705
BseDI CCNNGG 1 cut(s) 670
BseGI GGATG 2 cut(s) 180, 848
BseLI CCNNNNNNNGG 2 cut(s) 34, 151
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 1 cut(s) 479
BseXI GCAGC 2 cut(s) 34, 65
BshFI GGCC 2 cut(s) 565, 758
BshNI GGYRCC 1 cut(s) 836
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 2 cut(s) 283, 519
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 2 cut(s) 34, 151
BsmAI GTCTC 4 cut(s) 105, 108, 208, 478
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 2 cut(s) 565, 758
BsoBI CYCGRG 2 cut(s) 283, 519
Bsp1286I GDGCHC 1 cut(s) 549
Bsp1407I TGTACA 1 cut(s) 163
Bsp143I GATC 3 cut(s) 341, 600, 766
Bsp1720I GCTNAGC 1 cut(s) 23
BspACI CCGC 3 cut(s) 50, 179, 502
BspANI GGCC 2 cut(s) 565, 758
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 1 cut(s) 838
BspOI GCTAGC 1 cut(s) 95
BspQI GCTCTTC 1 cut(s) 79
BspT107I GGYRCC 1 cut(s) 836
BsrGI TGTACA 1 cut(s) 163
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 670
BssMI GATC 3 cut(s) 341, 600, 766
BssNI GRCGYC 1 cut(s) 505
BssT1I CCWWGG 1 cut(s) 670
Bst2UI CCWGG 2 cut(s) 320, 705
Bst4CI ACNGT 2 cut(s) 441, 489
Bst6I CTCTTC 4 cut(s) 79, 306, 336, 850
BstACI GRCGYC 1 cut(s) 505
BstAUI TGTACA 1 cut(s) 163
BstC8I GCNNGC 3 cut(s) 93, 500, 563
BstDEI CTNAG 4 cut(s) 23, 33, 123, 818
BstENI CCTNNNNNAGG 1 cut(s) 32
BstF5I GGATG 2 cut(s) 180, 848
BstKTI GATC 3 cut(s) 344, 603, 769
BstMAI GTCTC 4 cut(s) 105, 108, 208, 478
BstMBI GATC 3 cut(s) 341, 600, 766
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 2 cut(s) 320, 705
BstSCI CCNGG 2 cut(s) 318, 703
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 2 cut(s) 34, 65
BsuRI GGCC 2 cut(s) 565, 758
BtsCI GGATG 2 cut(s) 180, 848
Cac8I GCNNGC 3 cut(s) 93, 500, 563
CciI TCATGA 1 cut(s) 480
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 1 cut(s) 494
Csp6I GTAC 2 cut(s) 164, 667
CviAII CATG 3 cut(s) 473, 481, 535
CviQI GTAC 2 cut(s) 164, 667
DdeI CTNAG 4 cut(s) 23, 33, 123, 818
DpnI GATC 3 cut(s) 343, 602, 768
DpnII GATC 3 cut(s) 341, 600, 766
EaeI YGGCCR 1 cut(s) 756
Eam1104I CTCTTC 4 cut(s) 79, 306, 336, 850
EarI CTCTTC 4 cut(s) 79, 306, 336, 850
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 670
Eco147I AGGCCT 1 cut(s) 565
Eco24I GRGCYC 1 cut(s) 549
Eco32I GATATC 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 2 cut(s) 283, 519
EcoICRI GAGCTC 1 cut(s) 547
EcoNI CCTNNNNNAGG 1 cut(s) 32
EcoRII CCWGG 2 cut(s) 318, 703
EcoRV GATATC 1 cut(s) 290
EcoT14I CCWWGG 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 670
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 3 cut(s) 476, 484, 538
FaqI GGGAC 1 cut(s) 167
FatI CATG 3 cut(s) 472, 480, 534
Fnu4HI GCNGC 4 cut(s) 48, 51, 54, 503
FokI GGATG 2 cut(s) 187, 835
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 4 cut(s) 48, 51, 54, 503
FspBI CTAG 4 cut(s) 92, 339, 360, 671
GluI GCNGC 4 cut(s) 48, 51, 54, 503
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 2 cut(s) 565, 758
HapII CCGG 1 cut(s) 299
HgaI GACGC 1 cut(s) 494
Hin1I GRCGYC 1 cut(s) 505
Hin1II CATG 3 cut(s) 476, 484, 538
HinfI GANTC 4 cut(s) 59, 302, 469, 708
HpaII CCGG 1 cut(s) 299
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 2 cut(s) 40, 628
Hpy188I TCNGA 3 cut(s) 105, 204, 294
Hpy188III TCNNGA 6 cut(s) 213, 285, 308, 461, 481, 770
Hpy8I GTNNAC 2 cut(s) 40, 628
HpyAV CCTTC 3 cut(s) 645, 727, 850
HpyCH4III ACNGT 2 cut(s) 441, 489
HpyCH4V TGCA 3 cut(s) 596, 689, 829
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 4 cut(s) 23, 33, 123, 818
Hsp92I GRCGYC 1 cut(s) 505
Hsp92II CATG 3 cut(s) 476, 484, 538
Kzo9I GATC 3 cut(s) 341, 600, 766
LguI GCTCTTC 1 cut(s) 79
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 2 cut(s) 34, 65
LweI GCATC 1 cut(s) 315
MaeI CTAG 4 cut(s) 92, 339, 360, 671
MaeIII GTNAC 3 cut(s) 355, 489, 523
MalI GATC 3 cut(s) 343, 602, 768
MboI GATC 3 cut(s) 341, 600, 766
MboII GAAGA 4 cut(s) 66, 323, 323, 837
MhlI GDGCHC 1 cut(s) 549
MluCI AATT 4 cut(s) 4, 107, 588, 683
MlyI GAGTC 1 cut(s) 478
MnlI CCTC 7 cut(s) 38, 213, 307, 339, 457, 771, 787
MseI TTAA 1 cut(s) 859
MslI CAYNNNNRTG 1 cut(s) 834
MspA1I CMGCKG 1 cut(s) 502
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 2 cut(s) 320, 705
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 2 cut(s) 320, 705
MwoI GCNNNNNNNGC 1 cut(s) 53
NdeII GATC 3 cut(s) 341, 600, 766
NheI GCTAGC 1 cut(s) 91
NlaIII CATG 3 cut(s) 476, 484, 538
NlaIV GGNNCC 1 cut(s) 838
NmeAIII GCCGAG 1 cut(s) 784
PaeR7I CTCGAG 1 cut(s) 519
PagI TCATGA 1 cut(s) 480
PceI AGGCCT 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 502
PctI GAATGC 1 cut(s) 829
PfeI GAWTC 3 cut(s) 59, 302, 708
PfoI TCCNGGA 1 cut(s) 703
PkrI GCNGC 4 cut(s) 49, 52, 55, 504
PleI GAGTC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 549
Psp6I CCWGG 2 cut(s) 318, 703
PspGI CCWGG 2 cut(s) 318, 703
PspN4I GGNNCC 1 cut(s) 838
PspPI GGNCC 1 cut(s) 316
PspXI VCTCGAGB 1 cut(s) 519
RsaI GTAC 2 cut(s) 165, 668
RsaNI GTAC 2 cut(s) 164, 667
RseI CAYNNNNRTG 1 cut(s) 834
SacI GAGCTC 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 79
SaqAI TTAA 1 cut(s) 859
SatI GCNGC 4 cut(s) 48, 51, 54, 503
Sau3AI GATC 3 cut(s) 341, 600, 766
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 2 cut(s) 320, 705
SduI GDGCHC 1 cut(s) 549
SfaNI GCATC 1 cut(s) 315
SfcI CTRYAG 1 cut(s) 447
Sfr274I CTCGAG 1 cut(s) 519
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 834
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
Sse9I AATT 4 cut(s) 4, 107, 588, 683
SseBI AGGCCT 1 cut(s) 565
SsiI CCGC 3 cut(s) 50, 179, 502
SspMI CTAG 4 cut(s) 92, 339, 360, 671
SstI GAGCTC 1 cut(s) 549
StuI AGGCCT 1 cut(s) 565
StyD4I CCNGG 2 cut(s) 318, 703
StyI CCWWGG 1 cut(s) 670
TaaI ACNGT 2 cut(s) 441, 489
TaqI TCGA 2 cut(s) 212, 520
TasI AATT 4 cut(s) 4, 107, 588, 683
TatI WGTACW 1 cut(s) 163
TauI GCSGC 2 cut(s) 53, 505
TfiI GAWTC 3 cut(s) 59, 302, 708
Tru1I TTAA 1 cut(s) 859
Tru9I TTAA 1 cut(s) 859
TseI GCWGC 2 cut(s) 47, 53
TspDTI ATGAA 4 cut(s) 17, 445, 792, 837
TspGWI ACGGA 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 316
XagI CCTNNNNNAGG 1 cut(s) 32
XapI RAATTY 1 cut(s) 107
XhoI CTCGAG 1 cut(s) 519
XmaJI CCTAGG 1 cut(s) 670
XspI CTAG 4 cut(s) 92, 339, 360, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.