MD07G1305800.v1.1

Belongs to the phospholipid scramblase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0017071
Physical Location & Seq
Reverse (-)
36256102 .. 36261606
5505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1305800.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGAACTGGACAAGGTTTTCCACCAAGATTTACAAAACTTGTGCAGCAGATTCCAAAAGTGGATCCTCATTCTGTCATACTAGAAAACCCCGTCACAATGCTACTGACAACATTGAAAGCATTCCGGTCCGCTGTGGTTTTCAGTCTCAAAGAGGAAACTTACCTGCTACTTCATGGCCTCGGGATGGATGCTGTGTTGCTACTGTGGCGAAAGATTTTCAAGATTCTGAGCAGAGGTTGATGGGATCAAGAAATGTAATTTCGAGGTTTAATGGAAGTTCTGTGTGGTCAAAGCAGAGCCTGGTATCTTTGTCTCGTCAGTTTGGACACAAGGCTGGTAGTGGTGGTGATCCTGAATTGACTAGGGACTTCTTTATACAGCTCTGGGTTGCAGATAGGATAATGGAACAGTCTAAAAGAAACGGAAGAGGGAAAGCTGTGAAGTATAGTAAACAAACACCGTACCACTATTCATCCTACGGATGGTATTCAAGTGCACCTTTCAGTCAAGCAGCCTCTTTTGATGAGGATGAACCTGATATGAAGCAACCGCCTCCAAGTCAATCCGTCTCTGGATATCTGAAGCCAGAATCTCCAGAAGAGGCCCAGGTGATGCCTCTTCTTGCTAGATCAAATCTGCTGATTACTAGGGAGATAGAGTGGGTAAATCTTACTCTTGGATTTGAACAGGAAAATCGGTATGCAATAGTGGATGTGTGCTACCCAGAGTCACCTGTTGGTTTTATTCGTGAGAAGAGTCAGTTTATTGGTAGACAGTTGCTTCGCCTCAGGCGCCCTTTTGTTGCTTGTGTAACTGATGCCATGGGTAATGAGCTCTTTAGGGTTCGTAGGCCTTTTTGGTGGATAACCAGCTCAATTTATGTAGAGATCAACAGTAAGGAAATCGGTGTGGTTCACAGACGATGGCATCTTTGGAGGAGGGTGTATGATTTGTACCTAGGGAATAAGCAGTTTGCAGTTGTAGAAAATCCTGGCTTCTGGCATTGGACTTTTACTTTGAAGGACATTGACAGCAAAGTTTTGGCTGAGATTGATCGTGAATGGAGGGGTTTTGGCTTTGAGATTTTCACTGATGCGGGGCAATATGTGATTCGGTTTGGGAGTTCTGATGCCAGCTCCATTACTGGTTCTGCTAGATTGATTAATGAGTTGGAAGTCATTCGACCACTGACTCTGTTAGAGAGAGCTGTAACTGTTGCTCTTGCTGTATCGTTGGACAACGACTATTTCTCAAGACACGGTGGATGGGGATCGCCATTTTATGAAGTAGGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Scramblase

Protein Analysis

433

Amino Acids

49.44

Weight (kDa)

8.84

Isoelectric Point (pI)

58.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Scramblase PF03803 194 - 419 3.5e-60 Scramblase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04940
fragaria_vesca FvH4_7g33592
malus_domestica MD01G1233700.v1.1 MD07G1305800.v1.1
prunus_persica Prupe.2G323900_v2.0.a1
pyrus_communis pycom01g24200
rosa_chinensis RchiOBHm_Chr1g0383851
rosa_laevigata RLG00000026091
rosa_samantha Rh1AG468500 Rh1BG426200 Rh1CG438900 Rh1DG459000
rosa_wichuraiana Rw1G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 792
AccI GTMKAC 1 cut(s) 772
AciI CCGC 3 cut(s) 130, 551, 1097
AclWI GGATC 5 cut(s) 57, 70, 253, 344, 1279
AcuI CTGAAG 1 cut(s) 602
AcyI GRCGYC 1 cut(s) 793
AfaI GTAC 2 cut(s) 464, 956
AfiI CCNNNNNNNGG 2 cut(s) 185, 483
AgsI TTSAA 5 cut(s) 116, 221, 492, 686, 1021
AjnI CCWGG 3 cut(s) 300, 606, 991
AluBI AGCT 6 cut(s) 382, 437, 835, 873, 1137, 1208
AluI AGCT 6 cut(s) 382, 437, 835, 873, 1137, 1208
Alw21I GWGCWC 2 cut(s) 499, 837
Alw26I GTCTC 3 cut(s) 150, 318, 574
Alw44I GTGCAC 1 cut(s) 495
AlwI GGATC 5 cut(s) 57, 70, 253, 344, 1279
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 3 cut(s) 176, 603, 851
ApaLI GTGCAC 1 cut(s) 495
ApeKI GCWGC 2 cut(s) 44, 512
ArsI GACNNNNNNTTYG 4 cut(s) 765, 797, 1000, 1032
AseI ATTAAT 1 cut(s) 1164
Asp700I GAANNNNTTC 2 cut(s) 120, 1179
AspA2I CCTAGG 1 cut(s) 958
AspLEI GCGC 1 cut(s) 795
AspS9I GGNCC 2 cut(s) 127, 604
AsuHPI GGTGA 3 cut(s) 359, 622, 723
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 127
AvrII CCTAGG 1 cut(s) 958
AxyI CCTNAGG 1 cut(s) 788
BaeGI GKGCMC 1 cut(s) 499
BamHI GGATCC 1 cut(s) 62
BanI GGYRCC 1 cut(s) 792
BanII GRGCYC 1 cut(s) 837
Bbv12I GWGCWC 2 cut(s) 499, 837
BbvI GCAGC 2 cut(s) 56, 524
BccI CCATC 5 cut(s) 179, 235, 477, 918, 1260
BciT130I CCWGG 3 cut(s) 302, 608, 993
BcoDI GTCTC 3 cut(s) 150, 318, 574
BfaI CTAG 6 cut(s) 81, 363, 627, 648, 959, 1155
BfoI RGCGCY 1 cut(s) 796
BfuAI ACCTGC 1 cut(s) 172
BisI GCNGC 2 cut(s) 45, 513
BlnI CCTAGG 1 cut(s) 958
BlsI GCNGC 2 cut(s) 46, 514
Bme1390I CCNGG 3 cut(s) 302, 608, 993
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 127, 604
BmiI GGNNCC 2 cut(s) 64, 794
BmrFI CCNGG 3 cut(s) 302, 608, 993
BmsI GCATC 6 cut(s) 179, 603, 808, 937, 1084, 1120
BpmI CTGGAG 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 1237
BsaBI GATNNNNATC 1 cut(s) 1270
BsaHI GRCGYC 1 cut(s) 793
BsaJI CCNNGG 4 cut(s) 179, 606, 822, 958
BsaWI WCCGGW 1 cut(s) 124
BsaXI ACNNNNNCTCC 2 cut(s) 644, 674
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 483
Bse1I ACTGG 2 cut(s) 11, 1150
Bse21I CCTNAGG 1 cut(s) 788
Bse8I GATNNNNATC 1 cut(s) 1270
BseBI CCWGG 3 cut(s) 302, 608, 993
BseDI CCNNGG 4 cut(s) 179, 606, 822, 958
BseGI GGATG 7 cut(s) 190, 194, 473, 488, 535, 718, 1271
BseJI GATNNNNATC 1 cut(s) 1270
BseLI CCNNNNNNNGG 2 cut(s) 185, 483
BseMII CTCAG 3 cut(s) 219, 802, 1038
BseNI ACTGG 2 cut(s) 11, 1150
BseRI GAGGAG 1 cut(s) 952
BseSI GKGCMC 1 cut(s) 499
BseXI GCAGC 2 cut(s) 56, 524
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 3 cut(s) 178, 605, 853
BshNI GGYRCC 1 cut(s) 792
BsiHKAI GWGCWC 2 cut(s) 499, 837
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 1 cut(s) 125
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 2 cut(s) 185, 483
BsmAI GTCTC 3 cut(s) 150, 318, 574
BsmBI CGTCTC 1 cut(s) 574
BsmFI GGGAC 1 cut(s) 380
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 3 cut(s) 178, 605, 853
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 2 cut(s) 499, 837
Bsp143I GATC 7 cut(s) 62, 245, 349, 629, 888, 1054, 1271
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 3 cut(s) 130, 551, 1097
BspANI GGCC 3 cut(s) 178, 605, 853
BspCNI CTCAG 3 cut(s) 220, 801, 1039
BspLI GGNNCC 2 cut(s) 64, 794
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 5 cut(s) 57, 70, 253, 344, 1279
BspT107I GGYRCC 1 cut(s) 792
BsrI ACTGG 2 cut(s) 11, 1150
BssECI CCNNGG 4 cut(s) 179, 606, 822, 958
BssMI GATC 7 cut(s) 62, 245, 349, 629, 888, 1054, 1271
BssNI GRCGYC 1 cut(s) 793
BssT1I CCWWGG 2 cut(s) 822, 958
Bst2UI CCWGG 3 cut(s) 302, 608, 993
Bst4CI ACNGT 7 cut(s) 205, 411, 462, 777, 896, 1216, 1262
Bst6I CTCTTC 4 cut(s) 421, 594, 624, 749
BstACI GRCGYC 1 cut(s) 793
BstC8I GCNNGC 1 cut(s) 1135
BstDEI CTNAG 3 cut(s) 228, 788, 1047
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 7 cut(s) 190, 194, 473, 488, 535, 718, 1271
BstH2I RGCGCY 1 cut(s) 796
BstHHI GCGC 1 cut(s) 795
BstKTI GATC 7 cut(s) 65, 248, 352, 632, 891, 1057, 1274
BstMAI GTCTC 3 cut(s) 150, 318, 574
BstMBI GATC 7 cut(s) 62, 245, 349, 629, 888, 1054, 1271
BstMWI GCNNNNNNNGC 2 cut(s) 206, 792
BstNI CCWGG 3 cut(s) 302, 608, 993
BstSCI CCNGG 3 cut(s) 300, 606, 991
BstSLI GKGCMC 1 cut(s) 499
BstV1I GCAGC 2 cut(s) 56, 524
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
Bsu36I CCTNAGG 1 cut(s) 788
BsuRI GGCC 3 cut(s) 178, 605, 853
BtgI CCRYGG 1 cut(s) 822
BtsCI GGATG 7 cut(s) 190, 194, 473, 488, 535, 718, 1271
BtsIMutI CAGTG 2 cut(s) 1089, 1187
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 1135
CaiI CAGNNNCTG 1 cut(s) 301
CfoI GCGC 1 cut(s) 795
Cfr13I GGNCC 2 cut(s) 127, 604
CpoI CGGWCCG 1 cut(s) 127
Csp6I GTAC 2 cut(s) 463, 955
CspI CGGWCCG 1 cut(s) 127
CviAII CATG 2 cut(s) 174, 823
CviQI GTAC 2 cut(s) 463, 955
DdeI CTNAG 3 cut(s) 228, 788, 1047
DinI GGCGCC 1 cut(s) 794
DpnI GATC 7 cut(s) 64, 247, 351, 631, 890, 1056, 1273
DpnII GATC 7 cut(s) 62, 245, 349, 629, 888, 1054, 1271
Eam1104I CTCTTC 4 cut(s) 421, 594, 624, 749
EarI CTCTTC 4 cut(s) 421, 594, 624, 749
Ecl136II GAGCTC 1 cut(s) 835
Eco130I CCWWGG 2 cut(s) 822, 958
Eco147I AGGCCT 1 cut(s) 853
Eco24I GRGCYC 1 cut(s) 837
Eco32I GATATC 1 cut(s) 578
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 1 cut(s) 602
Eco81I CCTNAGG 1 cut(s) 788
Eco88I CYCGRG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 835
EcoRII CCWGG 3 cut(s) 300, 606, 991
EcoRV GATATC 1 cut(s) 578
EcoT14I CCWWGG 2 cut(s) 822, 958
EcoT38I GRGCYC 1 cut(s) 837
EgeI GGCGCC 1 cut(s) 794
EheI GGCGCC 1 cut(s) 794
ErhI CCWWGG 2 cut(s) 822, 958
Esp3I CGTCTC 1 cut(s) 574
FaeI CATG 2 cut(s) 177, 826
FalI AAGNNNNNCTT 2 cut(s) 484, 516
FaqI GGGAC 1 cut(s) 380
FatI CATG 2 cut(s) 173, 822
FauI CCCGC 1 cut(s) 1090
FblI GTMKAC 1 cut(s) 772
Fnu4HI GCNGC 2 cut(s) 45, 513
FokI GGATG 7 cut(s) 197, 201, 460, 495, 542, 725, 1278
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 2 cut(s) 45, 513
FspBI CTAG 6 cut(s) 81, 363, 627, 648, 959, 1155
GlaI GCGC 1 cut(s) 794
GluI GCNGC 2 cut(s) 45, 513
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 796
HaeIII GGCC 3 cut(s) 178, 605, 853
HapII CCGG 1 cut(s) 125
HhaI GCGC 1 cut(s) 795
Hin1I GRCGYC 1 cut(s) 793
Hin1II CATG 2 cut(s) 177, 826
Hin6I GCGC 1 cut(s) 793
HinP1I GCGC 1 cut(s) 793
HinfI GANTC 7 cut(s) 50, 224, 590, 728, 757, 1111, 1192
HpaII CCGG 1 cut(s) 125
HphI GGTGA 3 cut(s) 359, 622, 723
Hpy166II GTNNAC 4 cut(s) 452, 497, 773, 916
Hpy188I TCNGA 3 cut(s) 229, 582, 1129
Hpy188III TCNNGA 9 cut(s) 182, 221, 249, 353, 573, 596, 749, 1058, 1254
Hpy8I GTNNAC 4 cut(s) 452, 497, 773, 916
HpyAV CCTTC 1 cut(s) 1015
HpyCH4III ACNGT 7 cut(s) 205, 411, 462, 777, 896, 1216, 1262
HpyCH4V TGCA 5 cut(s) 44, 392, 497, 704, 977
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 792
HpyF3I CTNAG 3 cut(s) 228, 788, 1047
Hsp92I GRCGYC 1 cut(s) 793
Hsp92II CATG 2 cut(s) 177, 826
HspAI GCGC 1 cut(s) 793
KasI GGCGCC 1 cut(s) 792
Kzo9I GATC 7 cut(s) 62, 245, 349, 629, 888, 1054, 1271
LmnI GCTCC 1 cut(s) 1142
Lsp1109I GCAGC 2 cut(s) 56, 524
LweI GCATC 6 cut(s) 179, 603, 808, 937, 1084, 1120
MaeI CTAG 6 cut(s) 81, 363, 627, 648, 959, 1155
MaeIII GTNAC 4 cut(s) 92, 729, 811, 1210
MalI GATC 7 cut(s) 64, 247, 351, 631, 890, 1056, 1273
MboI GATC 7 cut(s) 62, 245, 349, 629, 888, 1054, 1271
MboII GAAGA 4 cut(s) 438, 611, 611, 766
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 2 cut(s) 499, 837
MluCI AATT 3 cut(s) 258, 356, 876
Mly113I GGCGCC 1 cut(s) 793
MlyI GAGTC 3 cut(s) 737, 766, 1186
MmeI TCCRAC 2 cut(s) 1152, 1215
MroXI GAANNNNTTC 2 cut(s) 120, 1179
MseI TTAA 2 cut(s) 270, 1164
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 3 cut(s) 302, 608, 993
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 3 cut(s) 302, 608, 993
MwoI GCNNNNNNNGC 2 cut(s) 206, 792
NarI GGCGCC 1 cut(s) 793
NcoI CCATGG 1 cut(s) 822
NdeII GATC 7 cut(s) 62, 245, 349, 629, 888, 1054, 1271
NlaIII CATG 2 cut(s) 177, 826
NlaIV GGNNCC 2 cut(s) 64, 794
NmuCI GTSAC 2 cut(s) 92, 729
PceI AGGCCT 1 cut(s) 853
PctI GAATGC 1 cut(s) 120
PdmI GAANNNNTTC 2 cut(s) 120, 1179
PfeI GAWTC 4 cut(s) 50, 224, 590, 1111
PkrI GCNGC 2 cut(s) 46, 514
PleI GAGTC 3 cut(s) 736, 765, 1186
PluTI GGCGCC 1 cut(s) 796
PpsI GAGTC 3 cut(s) 736, 765, 1186
PshBI ATTAAT 1 cut(s) 1164
Psp124BI GAGCTC 1 cut(s) 837
Psp6I CCWGG 3 cut(s) 300, 606, 991
PspGI CCWGG 3 cut(s) 300, 606, 991
PspN4I GGNNCC 2 cut(s) 64, 794
PspPI GGNCC 2 cut(s) 127, 604
PstNI CAGNNNCTG 1 cut(s) 301
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 2 cut(s) 464, 956
RsaNI GTAC 2 cut(s) 463, 955
Rsr2I CGGWCCG 1 cut(s) 127
RsrII CGGWCCG 1 cut(s) 127
SacI GAGCTC 1 cut(s) 837
SaqAI TTAA 2 cut(s) 270, 1164
SatI GCNGC 2 cut(s) 45, 513
Sau3AI GATC 7 cut(s) 62, 245, 349, 629, 888, 1054, 1271
Sau96I GGNCC 2 cut(s) 127, 604
SchI GAGTC 3 cut(s) 737, 766, 1186
ScrFI CCNGG 3 cut(s) 302, 608, 993
SduI GDGCHC 2 cut(s) 499, 837
SfaNI GCATC 6 cut(s) 179, 603, 808, 937, 1084, 1120
SfoI GGCGCC 1 cut(s) 794
SinI GGWCC 1 cut(s) 127
SmlI CTYRAG 1 cut(s) 1252
SmoI CTYRAG 1 cut(s) 1252
Sse9I AATT 3 cut(s) 258, 356, 876
SseBI AGGCCT 1 cut(s) 853
SsiI CCGC 3 cut(s) 130, 551, 1097
SspDI GGCGCC 1 cut(s) 792
SspMI CTAG 6 cut(s) 81, 363, 627, 648, 959, 1155
SstI GAGCTC 1 cut(s) 837
StuI AGGCCT 1 cut(s) 853
StyD4I CCNGG 3 cut(s) 300, 606, 991
StyI CCWWGG 2 cut(s) 822, 958
TaaI ACNGT 7 cut(s) 205, 411, 462, 777, 896, 1216, 1262
TaqI TCGA 2 cut(s) 263, 1183
TasI AATT 3 cut(s) 258, 356, 876
TfiI GAWTC 4 cut(s) 50, 224, 590, 1111
Tru1I TTAA 2 cut(s) 270, 1164
Tru9I TTAA 2 cut(s) 270, 1164
TscAI CASTG 2 cut(s) 1096, 1194
TseFI GTSAC 2 cut(s) 92, 729
TseI GCWGC 2 cut(s) 44, 512
Tsp45I GTSAC 2 cut(s) 92, 729
TspDTI ATGAA 6 cut(s) 17, 162, 462, 546, 557, 1299
TspGWI ACGGA 3 cut(s) 438, 495, 556
TspRI CASTG 2 cut(s) 1096, 1194
VneI GTGCAC 1 cut(s) 495
VpaK11BI GGWCC 1 cut(s) 127
VspI ATTAAT 1 cut(s) 1164
XmaJI CCTAGG 1 cut(s) 958
XmiI GTMKAC 1 cut(s) 772
XmnI GAANNNNTTC 2 cut(s) 120, 1179
XspI CTAG 6 cut(s) 81, 363, 627, 648, 959, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.