Rh1DG459000

Belongs to the phospholipid scramblase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0017071
Physical Location & Seq
Reverse (-)
66322939 .. 66326892
3954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG459000.1

Sequence Viewer

Length: 1218 bp
ATGAAGCAGGTAGATGAGGAAGCAGCCATGTACAGCAAAGAGAGAGAAGGTTGTCAACTGCATGTTTGCTGGAAATGGTTTTCTGGAGGAACAGGGCTCGTGTTAGGCAGGAACTGTAGTAAGGTAGAGAAACACAGAAAGCTCAAAGCTTTGAGAGAGCACCTGATCGGAAAAACAGAACCGGCTGAGCTCAACTCAACTGAACCGATGAATTGGAGAAGTAGTTGGCTTAGCCTCACTAAGGTTTACAAAGCTGCGGCAGCAGATTCCAAATGTGGCTCTTCCTTGTGGTCAGGGCTAGCTTTGTCTCGGAAATTTGGAGACAAGGCTAAGGCTGATACTGGGTTTGACCTGAATAGGGACTTCCTTGTACAGCTATGGATGGCGGATAAGGAAATGGAGCAGTTTCGGAGAAAATCGAGACGGAGGGCTGTTGAGCAAGATGAGAATAGCGTGGTTGGGAAGCAACCACCTGTGAGCCAATCCATCTCGGGATATCTGAAGCCGGAATCTCCAGAAGAGGTCCAGGTGATGCCTCTTCTTGCTAGATCAAATATGCTCGTTACTAGGGATATAGAGTGGGCAAATCTTATGCTTGGGTTTGAGCAGGAAAATCGGTATGCCATAGTAGATGTATGCTATCCACAGTCGCCTGTAGGTTTCATTCGTGAGGAGAGTCATGTTATCATGAGACAGTTACTTCGCCAGCGGCGTCCTTTTGTTGCTCGAGTAACTGATGGCATGGGAAATGAGCTCTTTAGGGTTCGCAGGCCTTTTTGGTGGATAAACAGCTCAATTTATGCAGAGATCAATGGTAAGGAAGTTGGTGTGGTTCACAGACGATGGCATTTGTGGAGAAGGATTTATGATTTGTACCTAGGGAATAAACAATTTGCAGTTGTTGAAAATCCTGGATTCTGGCATTGGACATTTACTTTGAAGGACATTGATGGGGGTGTGTTGGCCGAGATAGATCGTGATTGGAGGGGTTTTGGCTTTGAGCTGTTTACTGATGCTGGGCAATATGTGATTCGGTTTGGGAGCTCTGATCCCAGCTCCAAGATAGGTCTTGCTAGCCAGATTGAAGAGTTGGAAGTAGTTCGCCCATTGACTCTGTCAGAGAGAGCTGTAACTCTTGCTCTTGCTATATCGTTGGATAACGACTATTTTTCAAGACATGGTGGATGGGGAATACCTTATTTTGATGTAGGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Scramblase

Protein Analysis

405

Amino Acids

46.62

Weight (kDa)

8.46

Isoelectric Point (pI)

54.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Scramblase PF03803 169 - 392 5.4e-63 Scramblase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04940
fragaria_vesca FvH4_7g33592
malus_domestica MD01G1233700.v1.1 MD07G1305800.v1.1
prunus_persica Prupe.2G323900_v2.0.a1
pyrus_communis pycom01g24200
rosa_chinensis RchiOBHm_Chr1g0383851
rosa_laevigata RLG00000026091
rosa_samantha Rh1AG468500 Rh1BG426200 Rh1CG438900 Rh1DG459000
rosa_wichuraiana Rw1G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 257, 386, 709
AclWI GGATC 1 cut(s) 1043
AcoI YGGCCR 1 cut(s) 963
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 521
AcyI GRCGYC 1 cut(s) 712
AfaI GTAC 3 cut(s) 32, 372, 875
AfiI CCNNNNNNNGG 2 cut(s) 241, 358
AgsI TTSAA 4 cut(s) 905, 940, 1085, 1173
AjnI CCWGG 2 cut(s) 525, 910
Alw21I GWGCWC 4 cut(s) 162, 192, 756, 1046
Alw26I GTCTC 4 cut(s) 312, 315, 415, 685
AlwI GGATC 1 cut(s) 1043
AlwNI CAGNNNCTG 1 cut(s) 114
Ama87I CYCGRG 2 cut(s) 490, 726
AoxI GGCC 2 cut(s) 770, 963
ApeKI GCWGC 3 cut(s) 23, 254, 260
ApoI RAATTY 1 cut(s) 314
ArsI GACNNNNNNTTYG 4 cut(s) 684, 716, 919, 951
Asp700I GAANNNNTTC 1 cut(s) 1098
AspA2I CCTAGG 1 cut(s) 877
AspS9I GGNCC 1 cut(s) 523
AsuHPI GGTGA 1 cut(s) 541
AsuNHI GCTAGC 2 cut(s) 298, 1073
AvaI CYCGRG 2 cut(s) 490, 726
AvaII GGWCC 1 cut(s) 523
AvrII CCTAGG 1 cut(s) 877
BanII GRGCYC 4 cut(s) 99, 192, 756, 1046
BauI CACGAG 1 cut(s) 98
Bbv12I GWGCWC 4 cut(s) 162, 192, 756, 1046
BbvI GCAGC 3 cut(s) 35, 241, 272
BccI CCATC 6 cut(s) 376, 494, 731, 837, 944, 1179
BcgI CGANNNNNNTGC 2 cut(s) 596, 630
BciT130I CCWGG 2 cut(s) 527, 912
BcoDI GTCTC 4 cut(s) 312, 315, 415, 685
BfaI CTAG 5 cut(s) 299, 546, 567, 878, 1074
BfmI CTRYAG 2 cut(s) 115, 654
BisI GCNGC 5 cut(s) 24, 255, 258, 261, 710
BlnI CCTAGG 1 cut(s) 877
BlpI GCTNAGC 2 cut(s) 186, 230
BlsI GCNGC 5 cut(s) 25, 256, 259, 262, 711
Bme1390I CCNGG 2 cut(s) 527, 912
Bme18I GGWCC 1 cut(s) 523
BmeT110I CYCGRG 2 cut(s) 490, 726
BmgT120I GGNCC 1 cut(s) 523
BmrFI CCNGG 2 cut(s) 527, 912
BmrI ACTGGG 1 cut(s) 351
BmsI GCATC 2 cut(s) 522, 1003
BmtI GCTAGC 2 cut(s) 302, 1077
BmuI ACTGGG 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 179, 211
BpmI CTGGAG 2 cut(s) 105, 498
Bpu10I CCTNAGC 1 cut(s) 330
Bpu1102I GCTNAGC 2 cut(s) 186, 230
BsaHI GRCGYC 1 cut(s) 712
BsaJI CCNNGG 1 cut(s) 877
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 358
Bse118I RCCGGY 1 cut(s) 181
Bse1I ACTGG 1 cut(s) 346
BseBI CCWGG 2 cut(s) 527, 912
BseDI CCNNGG 1 cut(s) 877
BseGI GGATG 2 cut(s) 387, 1190
BseLI CCNNNNNNNGG 2 cut(s) 241, 358
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 1 cut(s) 346
BseRI GAGGAG 1 cut(s) 686
BseXI GCAGC 3 cut(s) 35, 241, 272
BseYI CCCAGC 2 cut(s) 1016, 1052
BshFI GGCC 2 cut(s) 772, 965
BsiHKAI GWGCWC 4 cut(s) 162, 192, 756, 1046
BsiHKCI CYCGRG 2 cut(s) 490, 726
BsiSI CCGG 2 cut(s) 182, 506
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 2 cut(s) 241, 358
BsmAI GTCTC 4 cut(s) 312, 315, 415, 685
BsmBI CGTCTC 1 cut(s) 415
BsmFI GGGAC 1 cut(s) 374
BsnI GGCC 2 cut(s) 772, 965
BsoBI CYCGRG 2 cut(s) 490, 726
Bsp1286I GDGCHC 5 cut(s) 99, 162, 192, 756, 1046
Bsp1407I TGTACA 2 cut(s) 30, 370
Bsp143I GATC 5 cut(s) 165, 548, 807, 973, 1048
Bsp1720I GCTNAGC 2 cut(s) 186, 230
BspACI CCGC 3 cut(s) 257, 386, 709
BspANI GGCC 2 cut(s) 772, 965
BspCNI CTCAG 1 cut(s) 178
BspHI TCATGA 1 cut(s) 687
BspOI GCTAGC 2 cut(s) 302, 1077
BspPI GGATC 1 cut(s) 1043
BspQI GCTCTTC 1 cut(s) 286
BsrFI RCCGGY 1 cut(s) 181
BsrGI TGTACA 2 cut(s) 30, 370
BsrI ACTGG 1 cut(s) 346
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 1 cut(s) 877
BssMI GATC 5 cut(s) 165, 548, 807, 973, 1048
BssNI GRCGYC 1 cut(s) 712
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 877
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 2 cut(s) 527, 912
Bst4CI ACNGT 3 cut(s) 116, 648, 696
Bst6I CTCTTC 4 cut(s) 286, 513, 543, 1080
BstACI GRCGYC 1 cut(s) 712
BstAUI TGTACA 2 cut(s) 30, 370
BstC8I GCNNGC 4 cut(s) 300, 707, 770, 1075
BstDEI CTNAG 4 cut(s) 186, 230, 240, 330
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 387, 1190
BstKTI GATC 5 cut(s) 168, 551, 810, 976, 1051
BstMAI GTCTC 4 cut(s) 312, 315, 415, 685
BstMBI GATC 5 cut(s) 165, 548, 807, 973, 1048
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNI CCWGG 2 cut(s) 527, 912
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 2 cut(s) 525, 910
BstSFI CTRYAG 2 cut(s) 115, 654
BstV1I GCAGC 3 cut(s) 35, 241, 272
BsuRI GGCC 2 cut(s) 772, 965
BtsCI GGATG 2 cut(s) 387, 1190
Cac8I GCNNGC 4 cut(s) 300, 707, 770, 1075
CaiI CAGNNNCTG 1 cut(s) 114
CciI TCATGA 1 cut(s) 687
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 523
CseI GACGC 1 cut(s) 701
Csp6I GTAC 3 cut(s) 31, 371, 874
CviAII CATG 6 cut(s) 28, 62, 680, 688, 742, 1178
CviQI GTAC 3 cut(s) 31, 371, 874
DdeI CTNAG 4 cut(s) 186, 230, 240, 330
DpnI GATC 5 cut(s) 167, 550, 809, 975, 1050
DpnII GATC 5 cut(s) 165, 548, 807, 973, 1048
EaeI YGGCCR 1 cut(s) 963
Eam1104I CTCTTC 4 cut(s) 286, 513, 543, 1080
EarI CTCTTC 4 cut(s) 286, 513, 543, 1080
EciI GGCGGA 1 cut(s) 401
Ecl136II GAGCTC 3 cut(s) 190, 754, 1044
Eco130I CCWWGG 1 cut(s) 877
Eco147I AGGCCT 1 cut(s) 772
Eco24I GRGCYC 4 cut(s) 99, 192, 756, 1046
Eco32I GATATC 1 cut(s) 497
Eco47I GGWCC 1 cut(s) 523
Eco53kI GAGCTC 3 cut(s) 190, 754, 1044
Eco57I CTGAAG 1 cut(s) 521
Eco88I CYCGRG 2 cut(s) 490, 726
EcoICRI GAGCTC 3 cut(s) 190, 754, 1044
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoRII CCWGG 2 cut(s) 525, 910
EcoRV GATATC 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 877
EcoT38I GRGCYC 4 cut(s) 99, 192, 756, 1046
ErhI CCWWGG 1 cut(s) 877
Esp3I CGTCTC 1 cut(s) 415
FaeI CATG 6 cut(s) 31, 65, 683, 691, 745, 1181
FaqI GGGAC 1 cut(s) 374
FatI CATG 6 cut(s) 27, 61, 679, 687, 741, 1177
Fnu4HI GCNGC 5 cut(s) 24, 255, 258, 261, 710
FokI GGATG 2 cut(s) 394, 1197
FriOI GRGCYC 4 cut(s) 99, 192, 756, 1046
Fsp4HI GCNGC 5 cut(s) 24, 255, 258, 261, 710
FspBI CTAG 5 cut(s) 299, 546, 567, 878, 1074
GluI GCNGC 5 cut(s) 24, 255, 258, 261, 710
GsaI CCCAGC 2 cut(s) 1020, 1056
GsuI CTGGAG 2 cut(s) 105, 498
HaeIII GGCC 2 cut(s) 772, 965
HapII CCGG 2 cut(s) 182, 506
HgaI GACGC 1 cut(s) 701
Hin1I GRCGYC 1 cut(s) 712
Hin1II CATG 6 cut(s) 31, 65, 683, 691, 745, 1181
HincII GTYRAC 1 cut(s) 56
HindII GTYRAC 1 cut(s) 56
HindIII AAGCTT 1 cut(s) 147
HinfI GANTC 6 cut(s) 266, 509, 676, 915, 1030, 1111
HpaII CCGG 2 cut(s) 182, 506
HphI GGTGA 1 cut(s) 541
Hpy166II GTNNAC 4 cut(s) 56, 247, 835, 1008
Hpy188I TCNGA 6 cut(s) 170, 312, 411, 501, 1048, 1120
Hpy188III TCNNGA 8 cut(s) 84, 420, 492, 515, 668, 688, 977, 1173
Hpy8I GTNNAC 4 cut(s) 56, 247, 835, 1008
HpyAV CCTTC 3 cut(s) 41, 852, 934
HpyCH4III ACNGT 3 cut(s) 116, 648, 696
HpyCH4V TGCA 3 cut(s) 61, 803, 896
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 4 cut(s) 186, 230, 240, 330
Hsp92I GRCGYC 1 cut(s) 712
Hsp92II CATG 6 cut(s) 31, 65, 683, 691, 745, 1181
Kzo9I GATC 5 cut(s) 165, 548, 807, 973, 1048
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 3 cut(s) 400, 1041, 1061
Lsp1109I GCAGC 3 cut(s) 35, 241, 272
LweI GCATC 2 cut(s) 522, 1003
MaeI CTAG 5 cut(s) 299, 546, 567, 878, 1074
MaeIII GTNAC 4 cut(s) 562, 696, 730, 1129
MalI GATC 5 cut(s) 167, 550, 809, 975, 1050
MboI GATC 5 cut(s) 165, 548, 807, 973, 1048
MboII GAAGA 4 cut(s) 273, 530, 530, 1097
MhlI GDGCHC 5 cut(s) 99, 162, 192, 756, 1046
MluCI AATT 4 cut(s) 211, 314, 795, 890
MlyI GAGTC 2 cut(s) 685, 1105
MmeI TCCRAC 2 cut(s) 1071, 1134
MnlI CCTC 8 cut(s) 10, 80, 245, 420, 514, 546, 664, 978
MroXI GAANNNNTTC 1 cut(s) 1098
MspA1I CMGCKG 1 cut(s) 709
MspI CCGG 2 cut(s) 182, 506
MspR9I CCNGG 2 cut(s) 527, 912
MvaI CCWGG 2 cut(s) 527, 912
MwoI GCNNNNNNNGC 1 cut(s) 260
NdeII GATC 5 cut(s) 165, 548, 807, 973, 1048
NheI GCTAGC 2 cut(s) 298, 1073
NlaIII CATG 6 cut(s) 31, 65, 683, 691, 745, 1181
NmeAIII GCCGAG 1 cut(s) 991
NspI RCATGY 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 726
PagI TCATGA 1 cut(s) 687
PceI AGGCCT 1 cut(s) 772
PciSI GCTCTTC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 709
PdmI GAANNNNTTC 1 cut(s) 1098
PfeI GAWTC 4 cut(s) 266, 509, 915, 1030
PflFI GACNNNGTC 1 cut(s) 1114
PfoI TCCNGGA 1 cut(s) 910
PkrI GCNGC 5 cut(s) 25, 256, 259, 262, 711
PleI GAGTC 2 cut(s) 684, 1105
PpsI GAGTC 2 cut(s) 684, 1105
Psp124BI GAGCTC 3 cut(s) 192, 756, 1046
Psp6I CCWGG 2 cut(s) 525, 910
PspFI CCCAGC 2 cut(s) 1016, 1052
PspGI CCWGG 2 cut(s) 525, 910
PspPI GGNCC 1 cut(s) 523
PspXI VCTCGAGB 1 cut(s) 726
PstNI CAGNNNCTG 1 cut(s) 114
PsyI GACNNNGTC 1 cut(s) 1114
RsaI GTAC 3 cut(s) 32, 372, 875
RsaNI GTAC 3 cut(s) 31, 371, 874
SacI GAGCTC 3 cut(s) 192, 756, 1046
SapI GCTCTTC 1 cut(s) 286
SatI GCNGC 5 cut(s) 24, 255, 258, 261, 710
Sau3AI GATC 5 cut(s) 165, 548, 807, 973, 1048
Sau96I GGNCC 1 cut(s) 523
SchI GAGTC 2 cut(s) 685, 1105
ScrFI CCNGG 2 cut(s) 527, 912
SduI GDGCHC 5 cut(s) 99, 162, 192, 756, 1046
SfaNI GCATC 2 cut(s) 522, 1003
SfcI CTRYAG 2 cut(s) 115, 654
Sfr274I CTCGAG 1 cut(s) 726
SinI GGWCC 1 cut(s) 523
SlaI CTCGAG 1 cut(s) 726
SmlI CTYRAG 1 cut(s) 726
SmoI CTYRAG 1 cut(s) 726
Sse9I AATT 4 cut(s) 211, 314, 795, 890
SseBI AGGCCT 1 cut(s) 772
SsiI CCGC 3 cut(s) 257, 386, 709
SspMI CTAG 5 cut(s) 299, 546, 567, 878, 1074
SstI GAGCTC 3 cut(s) 192, 756, 1046
StuI AGGCCT 1 cut(s) 772
StyD4I CCNGG 2 cut(s) 525, 910
StyI CCWWGG 1 cut(s) 877
TaaI ACNGT 3 cut(s) 116, 648, 696
TaqI TCGA 2 cut(s) 419, 727
TasI AATT 4 cut(s) 211, 314, 795, 890
TatI WGTACW 2 cut(s) 30, 370
TauI GCSGC 2 cut(s) 260, 712
TfiI GAWTC 4 cut(s) 266, 509, 915, 1030
TseI GCWGC 3 cut(s) 23, 254, 260
TspDTI ATGAA 3 cut(s) 17, 224, 652
TspGWI ACGGA 1 cut(s) 439
Tth111I GACNNNGTC 1 cut(s) 1114
VpaK11BI GGWCC 1 cut(s) 523
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 1 cut(s) 314
XceI RCATGY 1 cut(s) 65
XhoI CTCGAG 1 cut(s) 726
XmaJI CCTAGG 1 cut(s) 877
XmnI GAANNNNTTC 1 cut(s) 1098
XspI CTAG 5 cut(s) 299, 546, 567, 878, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.