Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009158

Physical Location & Seq

Genomic Coordinates Reverse (-)

337734 .. 345033

Gene Structure 7300 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1004800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2925 bp

ATGGCTTCCACAGCCTCCTTCTCTCCAAGCCCGCAGGCTCAAACGTACAGCTCCACATGGCGGAAACCGCCGAAGCTGAGCCACAAGTTCCTCTTCAGCTCAGCCTCCAACCGCCTCTTCTTCAAGCCCTCGCTCTCTTCCTCCACTCCTTTCCCCCTCAGCCTCAAGCCCCCTCCCGAACCGGTCCTCTCCCACCTGGTCCGAGACTCCGTCCAAGACAATCTCGTCGAAAATTCGATGAATTTACAGCTACCCATCAGAGAATTTCACCTAATTACGCCCGAGGAATCGGCGCCCAGGATTTTTATCCAGGACCCGCCGTGGATTGCCGCACTCTTTCTGAAAGGCATGTACAAGAAGGCCAACCAGGAGTTGAAATTGGAGTCGAAGGAAATCGACCGGCGGAAGTATAATTTGCTTCGGCGGCGGCAGATAAAGGCGGAGACGGAGGCGTGGGAGAGAATGGTGGAGGAGTACAAGGATTTGGAGCGAGTAATGCGGGAGAAGAAGCTGGCTCCGAGCTTGCCGTACGTGAAGACCTTGTTTTTGGGGTGGTTTGAGCCGCTGAGGGAGGCGATTGCGAGGGAGCAGCAGGTGCAGCAAACGAAGAAGCACAAGGCGGCGTTTGCGCCGCACATTGATTTGCTCCCGCCGGATAAGATGGCGCTGATTGTTATGCATAAGATGATGGGCTTGGTTATGGTGGGGAATCAAGATGGGTGTGTGCAGGTTGTTCAAGCTGCTGTTCATATTGGGATGGCCATTGAGCAGGAGGTTAGGATTCACAGTTTCTTGGAGAAAACCAAAAATTTCCAGAGAAAGAAGACTGCCGTTGCTGATGAAGACAGTCTGATTAAGGAGAAGGAGATACTGCGGAAACGTGTTAATGTTTTAATTAAAAGAAAAAGACTGAGGGAGGTGCAAAAACTCTTGGTAAAAGAAGAATTTAAACCCTGGGGTCGTGATACACAGGCTAAGCTGGGAAGTCGTCTGATAGAATTATTAACAGAAACAGCTTACGTACAACCTCCACTTAACCAGTTGGCTGATGGTCCACCTGATGTTAGACCGGCCTTTAGGCACAGATTTAAGGCTGCAGCAAAAACTCCAGGGCAGAAACTTGTGAAGAATTATGGAGTTATAGAATGTGATCCCCTAGTTCTTACTGGGCTTGATAAAACTGCTAAACATATGTTGATTCCTTATGTGCCAATGTTGGTGCCTCCCAGAAAGTGGAAAGGGTATGATAAGGGTGGTCACTTGTTCCTACCTTCTTATGTCATGCGTACTCATGGATCTAGGAAGCAGGTAGATACAATGAGGAATATTTCTGGAAAGCAGATGCAGAAAGTATTCGAGGCCCTAGATATGCTTGGAAGTACCAAATGGAGGGTGAATAAAAAAGTACTTAACGTGGTGGAGAGCATCTGGGCTAGAGGTGGCAACATTGCAGGCCTGGTTGATCGTGAAGATGTTCCAGTGCCAGATAAACCACCGCTTGAGGGTTTAACAGAAATTCAGGAATGGAAATGGAGTGTAAGAAAAGCAAAGAAGATTAACCAAGAAAGGCATTCTCTGCGATGTGACACAGAACTTAAGCTCTCTGTTGCTCGCAAGATGAAGGATGAGGAAGGCTTTTATTATCCTCACAATCTTGATTTTCGAGGCCGAGCATACCCAATGCATCCACATTTAAATCATTTGAGTTCTGATCTCTGTCGAGGAGTCCTTGAATTTGCTGAAGGACGACCATTAGGGAAGTCTGGTTTACGTTGGCTGAAGATTCATTTAGCAAACCTTTATGCGGGCGGTGTTGAAAAGCTTTCGTATGAGGAACGGCATGCATTTGTGGATCATCACATTGATGATATATTTGATTCAGCAACAAACCCTGTAAATGGAAACCGATGGTGGTTAACAGCTGAAGATCCTTTACAGTGCCTAGCGGCTTGTATCAATCTGTCAGAAGCGTTAAATAGCCCATCACCACATACTGTCATTTCTCATTTGCCCATCCACCAGGATGGTTCATGCAATGGCTTACAGCACTATGCAGCCTTGGGAAGAGATAGTTTGGAAGCAGCTGCAGTCAACTTAGTAGCTGGAGAGAAACCTGCCGATGTTTACTCAGAAATTGCTGCAAGGGTACATGAGATTATGGAAAGAGACAGCAATAAGGATCCGACTACAAGCCCAAATGCTTTATTAGCCCGAGTCTTAGTTAATCAGATTGACCGGAAATTGGTGAAACAGACTGTCATGACTTCAGTGTATGGAGTTACTTACGTTGGGGCACGCGAACAAATAAAAAGAAGATTAGAGGAGAAGGGTCTTATTACAGACGATAGACTACTTTTCACTGCAGCTTGCTATGCTGCTAAAGTTACTTTGGCTGCCCTTGGTGAGATATTCCAAGCTGCACGTGGTATAATGGGCTGGCTCGGTGATTGTGCCAAGGTGATTGCTTCAGAAAATCAACCTGTGCGTTGGACTACTCCTCTAGGTCTTCCTGTTGTGCAACCCTACTGTAAAAATGAACGACATCTTATAAGAACATCCCTTCAGGTTTTAGCCTTGCAGCGGGAGAGTAACTTGATAGATGTTAGAAAACAAAGGACTGCATTTCCTCCAAATTTTGTTCACTCACTTGACGGTTCACACATGATGATGACTGCACTTGCCTGCAGGGACGCGGGCCTGCGTTTTGCAGGGGTACATGACTCCTTCTGGACTCATGCATGTGACGTAGACCAAATGAACGAGATACTTCGGGAAAAGTTTATAGAGCTCTATAGCATGCCGATACTTGAAAATTTGCTAGAAAGCTTCCAAGCATCATATCCAGCGTTGACATTCCCTCCCCTCCCAGAAAGAGGTGACTTCGACTTGCAAGAGGTTCTGGAATCTCCATACTTTTTCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

975

Amino Acids

110.87

Weight (kDa)

9.22

Isoelectric Point (pI)

51.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	143 - 460	7.8e-75	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	583 - 948	2.8e-157	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009158)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24120 AT2G24120
fragaria_vesca	FvH4_1g00590 FvH4_1g00590 FvH4_1g00590 FvH4_1g00590
malus_domestica	MD02G1004800.v1.1
prunus_persica	Prupe.7G266200_v2.0.a1 Prupe.7G266200_v2.0.a1
pyrus_communis	pycom02g00340 pycom15g13310
rosa_chinensis	RchiOBHm_Chr2g0085221
rosa_laevigata	RLG00000015668
rosa_multiflora	Rmu_sc0008510.1_g000002 Rmu_sc0011989.1_g000007
rosa_roxburghii	Rroxscaffold_2G00155550
rosa_rugosa	Rorug01G0458400
rosa_samantha	Rh2AG006900 Rh2BG007500 Rh2CG008300 Rh2DG008600
rosa_wichuraiana	Rw2G000660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2549
AarI	CACCTGC	1 cut(s)	583
AasI	GACNNNNNNGTC	1 cut(s)	224
Acc36I	ACCTGC	4 cut(s)	583, 718, 1297, 2122
AccB1I	GGYRCC	2 cut(s)	292, 1219
AccB7I	CCANNNNNTGG	1 cut(s)	1233
AccI	GTMKAC	1 cut(s)	2748
AccII	CGCG	2 cut(s)	2298, 2693
AclWI	GGATC	6 cut(s)	1145, 1303, 1860, 1922, 2174, 2187
AcoI	YGGCCR	1 cut(s)	759
AcsI	RAATTY	9 cut(s)	232, 241, 263, 808, 944, 1515, 1733, 2632, 2812
AcuI	CTGAAG	7 cut(s)	79, 1761, 1799, 1944, 2250, 2451, 2546
AcvI	CACGTG	1 cut(s)	2423
AcyI	GRCGYC	1 cut(s)	293
AfiI	CCNNNNNNNGG	9 cut(s)	60, 1233, 1389, 1502, 1804, 1898, 2242, 2580, 2873
AflII	CTTAAG	1 cut(s)	1595
AflIII	ACRYGT	1 cut(s)	880
AgeI	ACCGGT	1 cut(s)	181
AgsI	TTSAA	7 cut(s)	124, 376, 737, 1733, 1817, 2810, 2920
AjnI	CCWGG	8 cut(s)	195, 296, 309, 366, 953, 1108, 1455, 2019
AjuI	GAANNNNNNNTTGG	2 cut(s)	101, 133
Alw21I	GWGCWC	1 cut(s)	2790
Alw26I	GTCTC	3 cut(s)	198, 437, 2160
AlwI	GGATC	6 cut(s)	1145, 1303, 1860, 1922, 2174, 2187
Ama87I	CYCGRG	2 cut(s)	281, 2211
AoxI	GGCC	7 cut(s)	360, 759, 1071, 1359, 1453, 1666, 2695
ApoI	RAATTY	9 cut(s)	232, 241, 263, 808, 944, 1515, 1733, 2632, 2812
ArsI	GACNNNNNNTTYG	2 cut(s)	529, 561
AsiGI	ACCGGT	1 cut(s)	181
Asp700I	GAANNNNTTC	2 cut(s)	1352, 1473
AspLEI	GCGC	3 cut(s)	295, 631, 667
AspS9I	GGNCC	6 cut(s)	184, 199, 313, 1052, 1360, 2695
AsuHPI	GGTGA	8 cut(s)	260, 1405, 1977, 2257, 2414, 2456, 2470, 2888
AvaI	CYCGRG	2 cut(s)	281, 2211
AvaII	GGWCC	4 cut(s)	184, 199, 313, 1052
BaeGI	GKGCMC	1 cut(s)	2296
BalI	TGGCCA	1 cut(s)	761
BamHI	GGATCC	1 cut(s)	2179
BanI	GGYRCC	2 cut(s)	292, 1219
BanII	GRGCYC	1 cut(s)	2790
BarI	GAAGNNNNNNTAC	2 cut(s)	1917, 1949
BbrPI	CACGTG	1 cut(s)	2423
BbsI	GAAGAC	4 cut(s)	542, 830, 849, 2498
Bbv12I	GWGCWC	1 cut(s)	2790
BbvCI	CCTCAGC	2 cut(s)	158, 566
BceAI	ACGGC	4 cut(s)	304, 511, 815, 1853
BciT130I	CCWGG	8 cut(s)	197, 298, 311, 368, 955, 1110, 1457, 2021
BcoDI	GTCTC	3 cut(s)	198, 437, 2160
BfaI	CTAG	7 cut(s)	1157, 1299, 1364, 1434, 1943, 2501, 2819
BfmI	CTRYAG	5 cut(s)	1095, 2085, 2361, 2683, 2791
BfoI	RGCGCY	2 cut(s)	296, 668
BfrI	CTTAAG	1 cut(s)	1595
BfuAI	ACCTGC	4 cut(s)	583, 718, 1297, 2122
BlpI	GCTNAGC	3 cut(s)	77, 100, 975
BmcAI	AGTACT	1 cut(s)	1407
Bme1390I	CCNGG	8 cut(s)	197, 298, 311, 368, 955, 1110, 1457, 2021
Bme18I	GGWCC	4 cut(s)	184, 199, 313, 1052
BmeT110I	CYCGRG	2 cut(s)	281, 2211
BmgT120I	GGNCC	6 cut(s)	184, 199, 313, 1052, 1360, 2695
BmiI	GGNNCC	5 cut(s)	294, 315, 516, 1221, 2181
BmrFI	CCNGG	8 cut(s)	197, 298, 311, 368, 955, 1110, 1457, 2021
BmrI	ACTGGG	1 cut(s)	1176
BmsI	GCATC	4 cut(s)	1332, 1434, 1693, 2843
BmuI	ACTGGG	1 cut(s)	1176
BpiI	GAAGAC	4 cut(s)	542, 830, 849, 2498
BpmI	CTGGAG	2 cut(s)	1092, 2124
Bpu10I	CCTNAGC	2 cut(s)	158, 566
Bpu1102I	GCTNAGC	3 cut(s)	77, 100, 975
BpuEI	CTTGAG	2 cut(s)	149, 1520
BsaAI	YACGTR	3 cut(s)	532, 1021, 2423
BsaBI	GATNNNNATC	1 cut(s)	305
BsaHI	GRCGYC	1 cut(s)	293
BsaJI	CCNNGG	9 cut(s)	282, 296, 320, 953, 954, 1109, 2058, 2398, 2454
BsaWI	WCCGGW	2 cut(s)	181, 2235
BsaXI	ACNNNNNCTCC	2 cut(s)	2842, 2872
Bsc4I	CCNNNNNNNGG	9 cut(s)	60, 1233, 1389, 1502, 1804, 1898, 2242, 2580, 2873
Bse118I	RCCGGY	3 cut(s)	181, 399, 1069
Bse1I	ACTGG	3 cut(s)	1039, 1171, 1478
Bse3DI	GCAATG	2 cut(s)	1446, 2041
Bse8I	GATNNNNATC	1 cut(s)	305
BseBI	CCWGG	8 cut(s)	197, 298, 311, 368, 955, 1110, 1457, 2021
BseDI	CCNNGG	9 cut(s)	282, 296, 320, 953, 954, 1109, 2058, 2398, 2454
BseGI	GGATG	6 cut(s)	762, 1630, 1684, 2013, 2029, 2555
BseJI	GATNNNNATC	1 cut(s)	305
BseLI	CCNNNNNNNGG	9 cut(s)	60, 1233, 1389, 1502, 1804, 1898, 2242, 2580, 2873
BseMI	GCAATG	2 cut(s)	1446, 2041
BseMII	CTCAG	6 cut(s)	68, 114, 172, 557, 902, 2142
BseNI	ACTGG	3 cut(s)	1039, 1171, 1478
BseRI	GAGGAG	4 cut(s)	485, 1737, 2336, 2487
BseSI	GKGCMC	1 cut(s)	2296
BseYI	CCCAGC	1 cut(s)	979
BsgI	GTGCAG	4 cut(s)	617, 746, 2403, 2658
Bsh1236I	CGCG	2 cut(s)	2298, 2693
Bsh1285I	CGRYCG	1 cut(s)	400
BshFI	GGCC	7 cut(s)	362, 761, 1073, 1361, 1455, 1668, 2697
BshNI	GGYRCC	2 cut(s)	292, 1219
BshTI	ACCGGT	1 cut(s)	181
BsiEI	CGRYCG	1 cut(s)	400
BsiHKAI	GWGCWC	1 cut(s)	2790
BsiHKCI	CYCGRG	2 cut(s)	281, 2211
BsiSI	CCGG	5 cut(s)	182, 400, 653, 1070, 2236
BsiWI	CGTACG	1 cut(s)	528
BslFI	GGGAC	1 cut(s)	2702
BslI	CCNNNNNNNGG	9 cut(s)	60, 1233, 1389, 1502, 1804, 1898, 2242, 2580, 2873
BsmAI	GTCTC	3 cut(s)	198, 437, 2160
BsmBI	CGTCTC	1 cut(s)	437
BsmFI	GGGAC	1 cut(s)	2702
BsmI	GAATGC	1 cut(s)	1570
BsnI	GGCC	7 cut(s)	362, 761, 1073, 1361, 1455, 1668, 2697
BsoBI	CYCGRG	2 cut(s)	281, 2211
Bsp1286I	GDGCHC	2 cut(s)	2296, 2790
Bsp1407I	TGTACA	1 cut(s)	351
Bsp143I	GATC	7 cut(s)	1150, 1295, 1462, 1711, 1852, 1927, 2179
Bsp1720I	GCTNAGC	3 cut(s)	77, 100, 975
BspANI	GGCC	7 cut(s)	362, 761, 1073, 1361, 1455, 1668, 2697
BspCNI	CTCAG	6 cut(s)	69, 113, 171, 558, 903, 2141
BspFNI	CGCG	2 cut(s)	2298, 2693
BspHI	TCATGA	1 cut(s)	2259
BspLI	GGNNCC	5 cut(s)	294, 315, 516, 1221, 2181
BspMAI	CTGCAG	4 cut(s)	1099, 2089, 2365, 2687
BspMI	ACCTGC	4 cut(s)	583, 718, 1297, 2122
BspPI	GGATC	6 cut(s)	1145, 1303, 1860, 1922, 2174, 2187
BspT107I	GGYRCC	2 cut(s)	292, 1219
BspTI	CTTAAG	1 cut(s)	1595
BsrDI	GCAATG	2 cut(s)	1446, 2041
BsrFI	RCCGGY	3 cut(s)	181, 399, 1069
BsrGI	TGTACA	1 cut(s)	351
BsrI	ACTGG	3 cut(s)	1039, 1171, 1478
BssAI	RCCGGY	3 cut(s)	181, 399, 1069
BssECI	CCNNGG	9 cut(s)	282, 296, 320, 953, 954, 1109, 2058, 2398, 2454
BssMI	GATC	7 cut(s)	1150, 1295, 1462, 1711, 1852, 1927, 2179
BssNI	GRCGYC	1 cut(s)	293
BssT1I	CCWWGG	3 cut(s)	2058, 2398, 2454
Bst2UI	CCWGG	8 cut(s)	197, 298, 311, 368, 955, 1110, 1457, 2021
Bst4CI	ACNGT	7 cut(s)	788, 848, 1938, 1996, 2257, 2528, 2654
Bst6I	CTCTTC	4 cut(s)	98, 122, 142, 2059
BstACI	GRCGYC	1 cut(s)	293
BstAFI	CTTAAG	1 cut(s)	1595
BstAPI	GCANNNNNTGC	2 cut(s)	595, 1576
BstAUI	TGTACA	1 cut(s)	351
BstBAI	YACGTR	3 cut(s)	532, 1021, 2423
BstDEI	CTNAG	9 cut(s)	77, 100, 158, 566, 911, 975, 2095, 2128, 2218
BstDSI	CCRYGG	1 cut(s)	320
BstF5I	GGATG	6 cut(s)	762, 1630, 1684, 2013, 2029, 2555
BstFNI	CGCG	2 cut(s)	2298, 2693
BstH2I	RGCGCY	2 cut(s)	296, 668
BstHHI	GCGC	3 cut(s)	295, 631, 667
BstKTI	GATC	7 cut(s)	1153, 1298, 1465, 1714, 1855, 1930, 2182
BstMAI	GTCTC	3 cut(s)	198, 437, 2160
BstMBI	GATC	7 cut(s)	1150, 1295, 1462, 1711, 1852, 1927, 2179
BstMCI	CGRYCG	1 cut(s)	400
BstNI	CCWGG	8 cut(s)	197, 298, 311, 368, 955, 1110, 1457, 2021
BstNSI	RCATGY	4 cut(s)	352, 1844, 2742, 2800
BstSCI	CCNGG	8 cut(s)	195, 296, 309, 366, 953, 1108, 1455, 2019
BstSFI	CTRYAG	5 cut(s)	1095, 2085, 2361, 2683, 2791
BstSLI	GKGCMC	1 cut(s)	2296
BstSNI	TACGTA	1 cut(s)	1021
BstUI	CGCG	2 cut(s)	2298, 2693
BstV2I	GAAGAC	4 cut(s)	542, 830, 849, 2498
BstX2I	RGATCY	3 cut(s)	1295, 1927, 2179
BstXI	CCANNNNNNTGG	1 cut(s)	2024
BstYI	RGATCY	3 cut(s)	1295, 1927, 2179
BsuRI	GGCC	7 cut(s)	362, 761, 1073, 1361, 1455, 1668, 2697
BtgI	CCRYGG	1 cut(s)	320
BtgZI	GCGATG	1 cut(s)	1594
BtsCI	GGATG	6 cut(s)	762, 1630, 1684, 2013, 2029, 2555
BtsI	GCAGTG	1 cut(s)	2358
BtsIMutI	CAGTG	4 cut(s)	1485, 1943, 2274, 2358
BveI	ACCTGC	4 cut(s)	583, 718, 1297, 2122
CciI	TCATGA	1 cut(s)	2259
CfoI	GCGC	3 cut(s)	295, 631, 667
Cfr10I	RCCGGY	3 cut(s)	181, 399, 1069
Cfr13I	GGNCC	6 cut(s)	184, 199, 313, 1052, 1360, 2695
CseI	GACGC	1 cut(s)	2699
CsiI	ACCWGGT	1 cut(s)	195
CspAI	ACCGGT	1 cut(s)	181
DdeI	CTNAG	9 cut(s)	77, 100, 158, 566, 911, 975, 2095, 2128, 2218
DinI	GGCGCC	1 cut(s)	294
DpnI	GATC	7 cut(s)	1152, 1297, 1464, 1713, 1854, 1929, 2181
DpnII	GATC	7 cut(s)	1150, 1295, 1462, 1711, 1852, 1927, 2179
DraI	TTTAAA	2 cut(s)	949, 1695
DrdI	GACNNNNNNGTC	1 cut(s)	224
DseDI	GACNNNNNNGTC	1 cut(s)	224
EaeI	YGGCCR	1 cut(s)	759
Eam1104I	CTCTTC	4 cut(s)	98, 122, 142, 2059
EarI	CTCTTC	4 cut(s)	98, 122, 142, 2059
EciI	GGCGGA	3 cut(s)	76, 418, 455
Ecl136II	GAGCTC	1 cut(s)	2788
Eco105I	TACGTA	1 cut(s)	1021
Eco130I	CCWWGG	3 cut(s)	2058, 2398, 2454
Eco147I	AGGCCT	1 cut(s)	1455
Eco24I	GRGCYC	1 cut(s)	2790
Eco47I	GGWCC	4 cut(s)	184, 199, 313, 1052
Eco53kI	GAGCTC	1 cut(s)	2788
Eco57I	CTGAAG	7 cut(s)	79, 1761, 1799, 1944, 2250, 2451, 2546
Eco72I	CACGTG	1 cut(s)	2423
Eco88I	CYCGRG	2 cut(s)	281, 2211
EcoICRI	GAGCTC	1 cut(s)	2788
EcoO109I	RGGNCCY	2 cut(s)	313, 1360
EcoRII	CCWGG	8 cut(s)	195, 296, 309, 366, 953, 1108, 1455, 2019
EcoT14I	CCWWGG	3 cut(s)	2058, 2398, 2454
EcoT22I	ATGCAT	4 cut(s)	681, 1686, 1846, 2740
EcoT38I	GRGCYC	1 cut(s)	2790
EgeI	GGCGCC	1 cut(s)	294
EheI	GGCGCC	1 cut(s)	294
ErhI	CCWWGG	3 cut(s)	2058, 2398, 2454
Esp3I	CGTCTC	1 cut(s)	437
FalI	AAGNNNNNCTT	2 cut(s)	77, 109
FaqI	GGGAC	1 cut(s)	2702
FauI	CCCGC	7 cut(s)	39, 324, 492, 657, 1798, 2574, 2686
FauNDI	CATATG	1 cut(s)	1191
FblI	GTMKAC	1 cut(s)	2748
FokI	GGATG	6 cut(s)	769, 1637, 1671, 2000, 2036, 2542
FriOI	GRGCYC	1 cut(s)	2790
FspBI	CTAG	7 cut(s)	1157, 1299, 1364, 1434, 1943, 2501, 2819
GlaI	GCGC	3 cut(s)	294, 630, 666
GsaI	CCCAGC	1 cut(s)	983
GsuI	CTGGAG	2 cut(s)	1092, 2124
HaeII	RGCGCY	2 cut(s)	296, 668
HaeIII	GGCC	7 cut(s)	362, 761, 1073, 1361, 1455, 1668, 2697
HapII	CCGG	5 cut(s)	182, 400, 653, 1070, 2236
HgaI	GACGC	1 cut(s)	2699
HhaI	GCGC	3 cut(s)	295, 631, 667
Hin1I	GRCGYC	1 cut(s)	293
Hin6I	GCGC	3 cut(s)	293, 629, 665
HinP1I	GCGC	3 cut(s)	293, 629, 665
HincII	GTYRAC	3 cut(s)	1917, 2092, 2850
HindII	GTYRAC	3 cut(s)	1917, 2092, 2850
HindIII	AAGCTT	2 cut(s)	1820, 2824
HpaI	GTTAAC	1 cut(s)	1917
HpaII	CCGG	5 cut(s)	182, 400, 653, 1070, 2236
HphI	GGTGA	8 cut(s)	260, 1405, 1977, 2257, 2414, 2456, 2470, 2888
Hpy166II	GTNNAC	9 cut(s)	1055, 1769, 1917, 2092, 2125, 2641, 2657, 2749, 2850
Hpy8I	GTNNAC	9 cut(s)	1055, 1769, 1917, 2092, 2125, 2641, 2657, 2749, 2850
Hpy99I	CGWCG	1 cut(s)	230
HpyCH4III	ACNGT	7 cut(s)	788, 848, 1938, 1996, 2257, 2528, 2654
HpyCH4IV	ACGT	9 cut(s)	44, 531, 880, 1020, 1413, 1771, 2286, 2422, 2745
HpyF3I	CTNAG	9 cut(s)	77, 100, 158, 566, 911, 975, 2095, 2128, 2218
HpySE526I	ACGT	9 cut(s)	44, 531, 880, 1020, 1413, 1771, 2286, 2422, 2745
Hsp92I	GRCGYC	1 cut(s)	293
HspAI	GCGC	3 cut(s)	293, 629, 665
KasI	GGCGCC	1 cut(s)	292
KspAI	GTTAAC	1 cut(s)	1917
Kzo9I	GATC	7 cut(s)	1150, 1295, 1462, 1711, 1852, 1927, 2179
LmnI	GCTCC	5 cut(s)	56, 487, 520, 586, 651
LweI	GCATC	4 cut(s)	1332, 1434, 1693, 2843
MabI	ACCWGGT	1 cut(s)	195
MaeI	CTAG	7 cut(s)	1157, 1299, 1364, 1434, 1943, 2501, 2819
MaeII	ACGT	9 cut(s)	44, 531, 880, 1020, 1413, 1771, 2286, 2422, 2745
MaeIII	GTNAC	7 cut(s)	1256, 1583, 2278, 2383, 2588, 2741, 2876
MalI	GATC	7 cut(s)	1152, 1297, 1464, 1713, 1854, 1929, 2181
MboI	GATC	7 cut(s)	1150, 1295, 1462, 1711, 1852, 1927, 2179
MflI	RGATCY	3 cut(s)	1295, 1927, 2179
MhlI	GDGCHC	2 cut(s)	2296, 2790
MlsI	TGGCCA	1 cut(s)	761
MluNI	TGGCCA	1 cut(s)	761
Mly113I	GGCGCC	1 cut(s)	293
MlyI	GAGTC	6 cut(s)	200, 392, 1734, 2223, 2714, 2725
MmeI	TCCRAC	3 cut(s)	132, 2207, 2468
Mox20I	TGGCCA	1 cut(s)	761
Mph1103I	ATGCAT	4 cut(s)	681, 1686, 1846, 2740
MroXI	GAANNNNTTC	2 cut(s)	1352, 1473
MscI	TGGCCA	1 cut(s)	761
MslI	CAYNNNNRTG	4 cut(s)	1863, 2022, 2666, 2739
Msp20I	TGGCCA	1 cut(s)	761
MspA1I	CMGCKG	4 cut(s)	565, 1922, 2084, 2581
MspCI	CTTAAG	1 cut(s)	1595
MspI	CCGG	5 cut(s)	182, 400, 653, 1070, 2236
MspR9I	CCNGG	8 cut(s)	197, 298, 311, 368, 955, 1110, 1457, 2021
Mva1269I	GAATGC	1 cut(s)	1570
MvaI	CCWGG	8 cut(s)	197, 298, 311, 368, 955, 1110, 1457, 2021
MvnI	CGCG	2 cut(s)	2298, 2693
NarI	GGCGCC	1 cut(s)	293
NdeI	CATATG	1 cut(s)	1191
NdeII	GATC	7 cut(s)	1150, 1295, 1462, 1711, 1852, 1927, 2179
NlaIV	GGNNCC	5 cut(s)	294, 315, 516, 1221, 2181
NmeAIII	GCCGAG	1 cut(s)	1694
NmuCI	GTSAC	4 cut(s)	1256, 1583, 2741, 2876
NsiI	ATGCAT	4 cut(s)	681, 1686, 1846, 2740
NspI	RCATGY	4 cut(s)	352, 1844, 2742, 2800
PacI	TTAATTAA	1 cut(s)	897
PaeI	GCATGC	2 cut(s)	1844, 2800
PagI	TCATGA	1 cut(s)	2259
PaqCI	CACCTGC	1 cut(s)	583
PasI	CCCWGGG	1 cut(s)	954
PceI	AGGCCT	1 cut(s)	1455
PctI	GAATGC	1 cut(s)	1570
PdmI	GAANNNNTTC	2 cut(s)	1352, 1473
PfeI	GAWTC	7 cut(s)	287, 709, 781, 1198, 1783, 1877, 2903
Pfl23II	CGTACG	1 cut(s)	528
PflFI	GACNNNGTC	1 cut(s)	209
PflMI	CCANNNNNTGG	1 cut(s)	1233
PfoI	TCCNGGA	1 cut(s)	309
PinAI	ACCGGT	1 cut(s)	181
PleI	GAGTC	6 cut(s)	200, 391, 1733, 2222, 2714, 2725
PluTI	GGCGCC	1 cut(s)	296
PmaCI	CACGTG	1 cut(s)	2423
PmlI	CACGTG	1 cut(s)	2423
PpsI	GAGTC	6 cut(s)	200, 391, 1733, 2222, 2714, 2725
Ppu21I	YACGTR	3 cut(s)	532, 1021, 2423
PpuMI	RGGWCCY	1 cut(s)	313
PsiI	TTATAA	1 cut(s)	2549
Psp124BI	GAGCTC	1 cut(s)	2790
Psp5II	RGGWCCY	1 cut(s)	313
Psp6I	CCWGG	8 cut(s)	195, 296, 309, 366, 953, 1108, 1455, 2019
PspCI	CACGTG	1 cut(s)	2423
PspFI	CCCAGC	1 cut(s)	979
PspGI	CCWGG	8 cut(s)	195, 296, 309, 366, 953, 1108, 1455, 2019
PspLI	CGTACG	1 cut(s)	528
PspN4I	GGNNCC	5 cut(s)	294, 315, 516, 1221, 2181
PspPI	GGNCC	6 cut(s)	184, 199, 313, 1052, 1360, 2695
PspPPI	RGGWCCY	1 cut(s)	313
PstI	CTGCAG	4 cut(s)	1099, 2089, 2365, 2687
PsuI	RGATCY	3 cut(s)	1295, 1927, 2179
PsyI	GACNNNGTC	1 cut(s)	209
PvuII	CAGCTG	2 cut(s)	1922, 2084
RseI	CAYNNNNRTG	4 cut(s)	1863, 2022, 2666, 2739
SacI	GAGCTC	1 cut(s)	2790
Sau3AI	GATC	7 cut(s)	1150, 1295, 1462, 1711, 1852, 1927, 2179
Sau96I	GGNCC	6 cut(s)	184, 199, 313, 1052, 1360, 2695
SbfI	CCTGCAGG	1 cut(s)	2687
ScaI	AGTACT	1 cut(s)	1407
SchI	GAGTC	6 cut(s)	200, 392, 1734, 2223, 2714, 2725
ScrFI	CCNGG	8 cut(s)	197, 298, 311, 368, 955, 1110, 1457, 2021
SdaI	CCTGCAGG	1 cut(s)	2687
SduI	GDGCHC	2 cut(s)	2296, 2790
SexAI	ACCWGGT	1 cut(s)	195
SfaNI	GCATC	4 cut(s)	1332, 1434, 1693, 2843
SfcI	CTRYAG	5 cut(s)	1095, 2085, 2361, 2683, 2791
SfoI	GGCGCC	1 cut(s)	294
SinI	GGWCC	4 cut(s)	184, 199, 313, 1052
SmiI	ATTTAAAT	1 cut(s)	1695
SmiMI	CAYNNNNRTG	4 cut(s)	1863, 2022, 2666, 2739
SmlI	CTYRAG	3 cut(s)	164, 1499, 1595
SmoI	CTYRAG	3 cut(s)	164, 1499, 1595
SnaBI	TACGTA	1 cut(s)	1021
SphI	GCATGC	2 cut(s)	1844, 2800
Sse8387I	CCTGCAGG	1 cut(s)	2687
SseBI	AGGCCT	1 cut(s)	1455
SspDI	GGCGCC	1 cut(s)	292
SspI	AATATT	1 cut(s)	1327
SspMI	CTAG	7 cut(s)	1157, 1299, 1364, 1434, 1943, 2501, 2819
SstI	GAGCTC	1 cut(s)	2790
StuI	AGGCCT	1 cut(s)	1455
StyD4I	CCNGG	8 cut(s)	195, 296, 309, 366, 953, 1108, 1455, 2019
StyI	CCWWGG	3 cut(s)	2058, 2398, 2454
SwaI	ATTTAAAT	1 cut(s)	1695
TaaI	ACNGT	7 cut(s)	788, 848, 1938, 1996, 2257, 2528, 2654
TaiI	ACGT	9 cut(s)	47, 534, 883, 1023, 1416, 1774, 2289, 2425, 2748
TaqI	TCGA	8 cut(s)	228, 236, 386, 396, 1356, 1663, 1720, 2883
TatI	WGTACW	3 cut(s)	351, 474, 1405
TauI	GCSGC	7 cut(s)	332, 427, 430, 565, 623, 634, 1949
TfiI	GAWTC	7 cut(s)	287, 709, 781, 1198, 1783, 1877, 2903
TscAI	CASTG	4 cut(s)	1485, 1943, 2274, 2365
TseFI	GTSAC	4 cut(s)	1256, 1583, 2741, 2876
Tsp45I	GTSAC	4 cut(s)	1256, 1583, 2741, 2876
TspDTI	ATGAA	8 cut(s)	254, 737, 855, 1634, 1775, 2019, 2550, 2771
TspGWI	ACGGA	2 cut(s)	199, 461
TspRI	CASTG	4 cut(s)	1485, 1943, 2274, 2365
Tth111I	GACNNNGTC	1 cut(s)	209
Van91I	CCANNNNNTGG	1 cut(s)	1233
Vha464I	CTTAAG	1 cut(s)	1595
VpaK11BI	GGWCC	4 cut(s)	184, 199, 313, 1052
XapI	RAATTY	9 cut(s)	232, 241, 263, 808, 944, 1515, 1733, 2632, 2812
XceI	RCATGY	4 cut(s)	352, 1844, 2742, 2800
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1046, 2420
XmiI	GTMKAC	1 cut(s)	2748
XmnI	GAANNNNTTC	2 cut(s)	1352, 1473
XspI	CTAG	7 cut(s)	1157, 1299, 1364, 1434, 1943, 2501, 2819
ZrmI	AGTACT	1 cut(s)	1407
Zsp2I	ATGCAT	4 cut(s)	681, 1686, 1846, 2740

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.