Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0009158

Physical Location & Seq

Genomic Coordinates Reverse (-)

628703 .. 640424

Gene Structure 11722 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG006900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2955 bp

ATGGCTTCCACAGCCTCCTCATTCTCTCCAACCCCTCCTCATGCTCAGGGCTGGAGAAGACCTCCCAAACTCCATAACCACAAGTCCCTCAAAAACCTTCACTTTCTCTTCAGCTCAACCTCCAACTCCAACACCCTCTTCTTCAAGCCCTCACTCTCTTCCTCCACTCATTTCCCTCTCAGCCTCAAAACCACTCCAGACCCAGTTCAGTTCCACCAGCTAAACGACTCCGTCCAAGACAATCTCGTCGAAAACCTCGAGAATTCCATGAAATTCCAGGTACCCATCACCGAATTTCACCTCACTAGGTCTCCAGAATCGTCCCCAAGAATCTTCATCCAAGACCCACCATGGATTGCGTCCCTTTTTCTGAAGGGTATGTTCAAGAAAGCCAATAAAGAATTGAAATTGGAGTCGAAGGAAATCGATAGGAGGAAGTATAATCTGCTGAGGAGGAGGCAGATTAAGGCGGAGACTGAGGCGTGGGAGAAGATGGTGGATGAGTATAAGGACTTGGAGAGAGTAATGCGTGAGAAGAAGCTGGCTCCGAGCTTGCCTTATGTAAAGGCCTTGTTTTTGGGGTGGTTTGAGCCATTGAGGGAGGCCATTGCTAAGGAGCAGAAGGCACAGCAAACCAAGAAGCACAAGGCGGCGTTTGCGCCGCACATTGATTTGTTGCCGGCGGATAAGATGGCACTGATTGTAATGCATAAGCTGATGGGGTTGGTGATGATGGGGAATCAAGATGGATGTGTTCAGGTGGTTCAGGCTGCCGTGCACATTGGTATGGCCATAGAGCAGGAGGTTCGGATTCACAGTTTCTTGGAGAAGACTAAAAGTCTCCAGAGAAAGAAGAGTGCGGCTGCGGATGAAGATGGTCTGAGCAAGGAGAAGGAGATACTGAGGAAACGTGTTAATGGTTTGATTAGAAGGAAAAGACTGGTTGAGGTGCAAAAGCTACTGAAAAAAGAAGATATGAAGCCCTGGGGTCGGGATACACAGGCTAAGCTGGGAAGTCGGCTGATAGAACTATTAACAGAAACAGCTCATGTACAACCTCCACTTGACCAATTGGCAGATGGTCCACCTGATGTTAGACCTGCATTTAGGCACAGACTAAAGGCTGCGGCAAAAAGTCCAGGGCAGAAACTTGTGAAGAACTATGGAGTTATTGAATGTGATCCCCTAGTTCTTACCGGACTTGATAAAACTGCTAAACATATGTTGATTCCTTATGTGCCAATGTTGGTGCCTCCCAAAAGATGGAAAGGGTATGACAAGGGTGGGCACTTATTCCTACCTTCTTATGTCATGCGTACCCATGGATCTAGGAAGCAGGTAGATACAATGAGAAATATTCCTGGAAGACAGATGCAGAAAGTATTTGAGGCTCTCGATATGCTTGGAAGCACCAAATGGCGGATTAATAAAAAGGTTCTTAATGTGGTGGAGAGCATCTGGGCTAGAGGTGGCAACATTGCAGGCCTGGTTGATCGTGAAGATGTTCCTGTACCAGAGAAACCACCATCTGAGGATTTAACAGAAATTCAGGAATGGAGATGGAGTGCAAGAAAAGCAAAGAAGCTTAACCAAGAGAGGCATTCTCAACGATGTGACACTGAACTTAAGCTCTCTGTCGCGCGCAGAATGAAAGACGAGGAAGGCTTTTATTATCCCCACAATCTTGATTTCCGAGGCCGAGCATACCCAATGCATCCCCATTTGAATCATTTGAGTTCTGATCTATGTCGAGGAGTCCTTGAATTTGCTGAAGGGCGGCCATTGGGGAAGTCCGGATTGCGATGGCTGAAGATTCATTTAGCAAACTTGTATTCAGGTGGTGTTGAAAAGCTTTCATACGATGGGCGTATAGCATTCGTGGATAACCACATTGATGATATATTTGATTCAGCAACAAACCCTGTTAATGGAAATCGTTGGTGGTTAACTGCTGAAGATCCTTTACAGTGCCTAGCTGCTTGTATCAATCTATCAGAAGCCTTAAATAGCCCATCACCACATACTGTCATTTCTCATTTACCCATCCATCAGGATGGTTCATGCAATGGCTTACAGCACTATGCAGCCTTGGGGAGAGATACTTTGGAAGCAGCTGCAGTCAACTTAGTTGCTGGAGAGAAACCTGCTGATGTGTATTCAGAAATAGCTGTGAGGGTACATGACATTATGGAGAGAGACAGCAATAAGGACCCAACTACCAGCCCAAATGCTTTATTGGCTAGAGTCTTAGTTGATCAGATTGATCGGAAATTGGTGAAACAGACTGTGATGACTTCAGTATATGGTGTTACTTATGTTGGGGCGCGTGAACAGATAAAAAGAAGATTAGAGGAGAAGGGTCTTATAACTGATGATAGACTATTGTTCACCGCAGCTTGTTATGCTGCTAAAGTGACACTGGCTGCCCTTGGAGAGATTTTCCAAGCTGCACGTGGCATAATGAGCTGGCTTGGTGATTGTGCCAAGGTAATTGCTTCAGAAAATCAGCCTGTTCGTTGGACGACTCCTCTAGGTCTTCCTGTTGTGCAACCGTACTGTAAAAGTGAACGACATCTTATAAGAACATCCCTTCAGGTTCTGGCCTTGCAGCGGGAGAGTAACTTGGTAGATGTAAGGAAACAGAGAACTGCATTTCCTCCAAATTTTGTACACTCACTTGATGGTTCACACATGATGATGACAGCTCTTGCCTGCAGGGATGTTGGCATACGCTTTGCAGGGGTGCATGACTCCTTCTGGACTCATGCATGTGACGTGGATCTAATGAACCAGATACTTCGGGAAAACTTTGTGGAGCTCTATAGCAAGCCGATACTTGAGAATTTGCTTAAAAGCTTTCAATCAGCGTATCCAGAATTGACGTTTCCTCCCCTCCCAGAAAGAGGTGACTTTGACCTGCAAGAGGTTCTAAAATCTCCATACTTTTTCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

984

Amino Acids

111.73

Weight (kDa)

9.32

Isoelectric Point (pI)

50.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	153 - 470	6e-75	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	593 - 958	4.1e-156	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009158)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24120 AT2G24120
fragaria_vesca	FvH4_1g00590 FvH4_1g00590 FvH4_1g00590 FvH4_1g00590
malus_domestica	MD02G1004800.v1.1
prunus_persica	Prupe.7G266200_v2.0.a1 Prupe.7G266200_v2.0.a1
pyrus_communis	pycom02g00340 pycom15g13310
rosa_chinensis	RchiOBHm_Chr2g0085221
rosa_laevigata	RLG00000015668
rosa_multiflora	Rmu_sc0008510.1_g000002 Rmu_sc0011989.1_g000007
rosa_roxburghii	Rroxscaffold_2G00155550
rosa_rugosa	Rorug01G0458400
rosa_samantha	Rh2AG006900 Rh2BG007500 Rh2CG008300 Rh2DG008600
rosa_wichuraiana	Rw2G000660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2366, 2579
AasI	GACNNNNNNGTC	1 cut(s)	245
Acc36I	ACCTGC	4 cut(s)	1108, 1327, 2152, 2925
Acc65I	GGTACC	1 cut(s)	280
AccB1I	GGYRCC	2 cut(s)	280, 1249
AccB7I	CCANNNNNTGG	1 cut(s)	1263
AccII	CGCG	3 cut(s)	1640, 1642, 2326
AccIII	TCCGGA	1 cut(s)	1793
AclWI	GGATC	4 cut(s)	1175, 1333, 1952, 2787
AcoI	YGGCCR	2 cut(s)	789, 1778
AcsI	RAATTY	7 cut(s)	262, 272, 293, 1545, 1763, 2662, 2842
AcuI	CTGAAG	8 cut(s)	94, 392, 1791, 1829, 1974, 2280, 2481, 2576
AcvI	CACGTG	1 cut(s)	2453
AfaI	GTAC	7 cut(s)	282, 1053, 1318, 1512, 2178, 2555, 2670
AfiI	CCNNNNNNNGG	7 cut(s)	990, 1263, 1419, 1928, 2610, 2903, 2923
AflII	CTTAAG	1 cut(s)	1625
AflIII	ACRYGT	1 cut(s)	910
AgsI	TTSAA	9 cut(s)	145, 385, 406, 1175, 1726, 1763, 1847, 2861, 2950
AhdI	GACNNNNNGTC	1 cut(s)	837
AjiI	CACGTC	1 cut(s)	2776
AjnI	CCWGG	5 cut(s)	276, 983, 1138, 1360, 1485
AjuI	GAANNNNNNNTTGG	2 cut(s)	122, 154
AloI	GAACNNNNNNTCC	2 cut(s)	2638, 2670
Alw21I	GWGCWC	2 cut(s)	780, 2820
Alw26I	GTCTC	4 cut(s)	315, 467, 845, 2190
Alw44I	GTGCAC	1 cut(s)	776
AlwI	GGATC	4 cut(s)	1175, 1333, 1952, 2787
AlwNI	CAGNNNCTG	1 cut(s)	2599
Ama87I	CYCGRG	1 cut(s)	257
Aor13HI	TCCGGA	1 cut(s)	1793
AoxI	GGCC	7 cut(s)	567, 603, 789, 1483, 1696, 1778, 2601
ApaLI	GTGCAC	1 cut(s)	776
ApoI	RAATTY	7 cut(s)	262, 272, 293, 1545, 1763, 2662, 2842
AseI	ATTAAT	1 cut(s)	1425
Asp700I	GAANNNNTTC	1 cut(s)	1503
Asp718I	GGTACC	1 cut(s)	280
AspLEI	GCGC	4 cut(s)	661, 1642, 1644, 2326
AspS9I	GGNCC	2 cut(s)	1082, 2209
AsuHPI	GGTGA	8 cut(s)	280, 290, 739, 2007, 2287, 2380, 2486, 2918
AvaI	CYCGRG	1 cut(s)	257
AvaII	GGWCC	2 cut(s)	1082, 2209
BaeGI	GKGCMC	2 cut(s)	780, 1290
BalI	TGGCCA	1 cut(s)	791
BanI	GGYRCC	2 cut(s)	280, 1249
BanII	GRGCYC	1 cut(s)	2820
BarI	GAAGNNNNNNTAC	2 cut(s)	1947, 1979
BbrPI	CACGTG	1 cut(s)	2453
BbsI	GAAGAC	4 cut(s)	64, 836, 1372, 2528
Bbv12I	GWGCWC	2 cut(s)	780, 2820
BbvCI	CCTCAGC	1 cut(s)	449
BceAI	ACGGC	1 cut(s)	758
BcgI	CGANNNNNNTGC	2 cut(s)	788, 822
BciT130I	CCWGG	5 cut(s)	278, 985, 1140, 1362, 1487
BciVI	GTATCC	2 cut(s)	988, 2880
BclI	TGATCA	1 cut(s)	2254
BcoDI	GTCTC	4 cut(s)	315, 467, 845, 2190
BfaI	CTAG	7 cut(s)	306, 1187, 1329, 1464, 1973, 2241, 2531
BfmI	CTRYAG	3 cut(s)	2115, 2713, 2821
BfrI	CTTAAG	1 cut(s)	1625
BfuAI	ACCTGC	4 cut(s)	1108, 1327, 2152, 2925
BfuI	GTATCC	2 cut(s)	988, 2880
BlpI	GCTNAGC	1 cut(s)	1005
Bme1390I	CCNGG	5 cut(s)	278, 985, 1140, 1362, 1487
Bme18I	GGWCC	2 cut(s)	1082, 2209
BmeRI	GACNNNNNGTC	1 cut(s)	837
BmeT110I	CYCGRG	1 cut(s)	257
BmgBI	CACGTC	1 cut(s)	2776
BmgT120I	GGNCC	2 cut(s)	1082, 2209
BmiI	GGNNCC	4 cut(s)	282, 546, 1251, 2211
BmrFI	CCNGG	5 cut(s)	278, 985, 1140, 1362, 1487
BmrI	ACTGGG	1 cut(s)	197
BmsI	GCATC	3 cut(s)	1362, 1464, 1723
BmuI	ACTGGG	1 cut(s)	197
BpiI	GAAGAC	4 cut(s)	64, 836, 1372, 2528
BplI	GAGNNNNNCTC	4 cut(s)	46, 78, 1588, 1620
BpmI	CTGGAG	5 cut(s)	73, 180, 297, 827, 2154
Bpu10I	CCTNAGC	3 cut(s)	45, 449, 612
Bpu1102I	GCTNAGC	1 cut(s)	1005
BpuEI	CTTGAG	1 cut(s)	2858
Bsa29I	ATCGAT	1 cut(s)	426
BsaAI	YACGTR	1 cut(s)	2453
BsaI	GGTCTC	1 cut(s)	315
BsaJI	CCNNGG	9 cut(s)	350, 983, 984, 1139, 1321, 1693, 2088, 2428, 2484
BsaWI	WCCGGW	2 cut(s)	1196, 1793
BsaXI	ACNNNNNCTCC	6 cut(s)	295, 325, 1549, 1579, 2872, 2902
Bsc4I	CCNNNNNNNGG	7 cut(s)	990, 1263, 1419, 1928, 2610, 2903, 2923
Bse118I	RCCGGY	1 cut(s)	679
Bse1I	ACTGG	3 cut(s)	203, 945, 2424
Bse3DI	GCAATG	3 cut(s)	606, 1476, 2071
BseAI	TCCGGA	1 cut(s)	1793
BseBI	CCWGG	5 cut(s)	278, 985, 1140, 1362, 1487
BseCI	ATCGAT	1 cut(s)	426
BseDI	CCNNGG	9 cut(s)	350, 983, 984, 1139, 1321, 1693, 2088, 2428, 2484
BseGI	GGATG	9 cut(s)	336, 505, 755, 874, 1714, 2043, 2059, 2585, 2725
BseLI	CCNNNNNNNGG	7 cut(s)	990, 1263, 1419, 1928, 2610, 2903, 2923
BseMI	GCAATG	3 cut(s)	606, 1476, 2071
BseMII	CTCAG	7 cut(s)	59, 193, 440, 468, 872, 893, 1521
BseNI	ACTGG	3 cut(s)	203, 945, 2424
BsePI	GCGCGC	1 cut(s)	1640
BseRI	GAGGAG	7 cut(s)	7, 27, 466, 469, 1767, 2366, 2517
BseSI	GKGCMC	2 cut(s)	780, 1290
BseYI	CCCAGC	1 cut(s)	1009
BsgI	GTGCAG	1 cut(s)	2433
Bsh1236I	CGCG	3 cut(s)	1640, 1642, 2326
BshFI	GGCC	7 cut(s)	569, 605, 791, 1485, 1698, 1780, 2603
BshNI	GGYRCC	2 cut(s)	280, 1249
BshVI	ATCGAT	1 cut(s)	426
BsiHKAI	GWGCWC	2 cut(s)	780, 2820
BsiHKCI	CYCGRG	1 cut(s)	257
BsiSI	CCGG	3 cut(s)	680, 1197, 1794
BslFI	GGGAC	3 cut(s)	70, 307, 346
BslI	CCNNNNNNNGG	7 cut(s)	990, 1263, 1419, 1928, 2610, 2903, 2923
BsmAI	GTCTC	4 cut(s)	315, 467, 845, 2190
BsmFI	GGGAC	3 cut(s)	70, 307, 346
BsmI	GAATGC	2 cut(s)	1600, 1874
BsnI	GGCC	7 cut(s)	569, 605, 791, 1485, 1698, 1780, 2603
Bso31I	GGTCTC	1 cut(s)	315
BsoBI	CYCGRG	1 cut(s)	257
Bsp1286I	GDGCHC	3 cut(s)	780, 1290, 2820
Bsp13I	TCCGGA	1 cut(s)	1793
Bsp1407I	TGTACA	2 cut(s)	1051, 2668
Bsp143I	GATC	8 cut(s)	1180, 1325, 1492, 1741, 1957, 2254, 2263, 2779
Bsp1720I	GCTNAGC	1 cut(s)	1005
Bsp19I	CCATGG	2 cut(s)	350, 1321
BspANI	GGCC	7 cut(s)	569, 605, 791, 1485, 1698, 1780, 2603
BspCNI	CTCAG	7 cut(s)	58, 192, 441, 469, 873, 894, 1522
BspDI	ATCGAT	1 cut(s)	426
BspEI	TCCGGA	1 cut(s)	1793
BspFNI	CGCG	3 cut(s)	1640, 1642, 2326
BspLI	GGNNCC	4 cut(s)	282, 546, 1251, 2211
BspMAI	CTGCAG	2 cut(s)	2119, 2717
BspMI	ACCTGC	4 cut(s)	1108, 1327, 2152, 2925
BspPI	GGATC	4 cut(s)	1175, 1333, 1952, 2787
BspT107I	GGYRCC	2 cut(s)	280, 1249
BspTI	CTTAAG	1 cut(s)	1625
BspTNI	GGTCTC	1 cut(s)	315
BsrDI	GCAATG	3 cut(s)	606, 1476, 2071
BsrFI	RCCGGY	1 cut(s)	679
BsrGI	TGTACA	2 cut(s)	1051, 2668
BsrI	ACTGG	3 cut(s)	203, 945, 2424
BssAI	RCCGGY	1 cut(s)	679
BssECI	CCNNGG	9 cut(s)	350, 983, 984, 1139, 1321, 1693, 2088, 2428, 2484
BssHII	GCGCGC	1 cut(s)	1640
BssMI	GATC	8 cut(s)	1180, 1325, 1492, 1741, 1957, 2254, 2263, 2779
BssT1I	CCWWGG	5 cut(s)	350, 1321, 2088, 2428, 2484
Bst2UI	CCWGG	5 cut(s)	278, 985, 1140, 1362, 1487
Bst4CI	ACNGT	6 cut(s)	818, 1968, 2026, 2287, 2553, 2558
Bst6I	CTCTTC	4 cut(s)	113, 143, 163, 848
BstAFI	CTTAAG	1 cut(s)	1625
BstAUI	TGTACA	2 cut(s)	1051, 2668
BstBAI	YACGTR	1 cut(s)	2453
BstC8I	GCNNGC	8 cut(s)	543, 554, 681, 1483, 1642, 2468, 2713, 2828
BstDSI	CCRYGG	2 cut(s)	350, 1321
BstENI	CCTNNNNNAGG	1 cut(s)	2921
BstF5I	GGATG	9 cut(s)	336, 505, 755, 874, 1714, 2043, 2059, 2585, 2725
BstFNI	CGCG	3 cut(s)	1640, 1642, 2326
BstHHI	GCGC	4 cut(s)	661, 1642, 1644, 2326
BstKTI	GATC	8 cut(s)	1183, 1328, 1495, 1744, 1960, 2257, 2266, 2782
BstMAI	GTCTC	4 cut(s)	315, 467, 845, 2190
BstMBI	GATC	8 cut(s)	1180, 1325, 1492, 1741, 1957, 2254, 2263, 2779
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 656, 1574, 2237, 2402, 2463
BstNI	CCWGG	5 cut(s)	278, 985, 1140, 1362, 1487
BstNSI	RCATGY	1 cut(s)	2772
BstSCI	CCNGG	5 cut(s)	276, 983, 1138, 1360, 1485
BstSFI	CTRYAG	3 cut(s)	2115, 2713, 2821
BstSLI	GKGCMC	2 cut(s)	780, 1290
BstUI	CGCG	3 cut(s)	1640, 1642, 2326
BstV2I	GAAGAC	4 cut(s)	64, 836, 1372, 2528
BstX2I	RGATCY	3 cut(s)	1325, 1957, 2779
BstXI	CCANNNNNNTGG	1 cut(s)	2054
BstYI	RGATCY	3 cut(s)	1325, 1957, 2779
Bsu15I	ATCGAT	1 cut(s)	426
BsuI	GTATCC	2 cut(s)	988, 2880
BsuRI	GGCC	7 cut(s)	569, 605, 791, 1485, 1698, 1780, 2603
BsuTUI	ATCGAT	1 cut(s)	426
BtgI	CCRYGG	2 cut(s)	350, 1321
BtgZI	GCGATG	1 cut(s)	1816
BtrI	CACGTC	1 cut(s)	2776
BtsCI	GGATG	9 cut(s)	336, 505, 755, 874, 1714, 2043, 2059, 2585, 2725
BtsIMutI	CAGTG	4 cut(s)	695, 1617, 1973, 2417
BveI	ACCTGC	4 cut(s)	1108, 1327, 2152, 2925
Cac8I	GCNNGC	8 cut(s)	543, 554, 681, 1483, 1642, 2468, 2713, 2828
CaiI	CAGNNNCTG	1 cut(s)	2599
CfoI	GCGC	4 cut(s)	661, 1642, 1644, 2326
Cfr10I	RCCGGY	1 cut(s)	679
Cfr13I	GGNCC	2 cut(s)	1082, 2209
ClaI	ATCGAT	1 cut(s)	426
CseI	GACGC	1 cut(s)	348
Csp6I	GTAC	7 cut(s)	281, 1052, 1317, 1511, 2177, 2554, 2669
CviQI	GTAC	7 cut(s)	281, 1052, 1317, 1511, 2177, 2554, 2669
DpnI	GATC	8 cut(s)	1182, 1327, 1494, 1743, 1959, 2256, 2265, 2781
DpnII	GATC	8 cut(s)	1180, 1325, 1492, 1741, 1957, 2254, 2263, 2779
DrdI	GACNNNNNNGTC	1 cut(s)	245
DriI	GACNNNNNGTC	1 cut(s)	837
DseDI	GACNNNNNNGTC	1 cut(s)	245
EaeI	YGGCCR	2 cut(s)	789, 1778
Eam1104I	CTCTTC	4 cut(s)	113, 143, 163, 848
Eam1105I	GACNNNNNGTC	1 cut(s)	837
EarI	CTCTTC	4 cut(s)	113, 143, 163, 848
EciI	GGCGGA	3 cut(s)	485, 698, 1435
Ecl136II	GAGCTC	1 cut(s)	2818
Eco130I	CCWWGG	5 cut(s)	350, 1321, 2088, 2428, 2484
Eco147I	AGGCCT	2 cut(s)	569, 1485
Eco24I	GRGCYC	1 cut(s)	2820
Eco31I	GGTCTC	1 cut(s)	315
Eco47I	GGWCC	2 cut(s)	1082, 2209
Eco53kI	GAGCTC	1 cut(s)	2818
Eco57I	CTGAAG	8 cut(s)	94, 392, 1791, 1829, 1974, 2280, 2481, 2576
Eco72I	CACGTG	1 cut(s)	2453
Eco88I	CYCGRG	1 cut(s)	257
EcoICRI	GAGCTC	1 cut(s)	2818
EcoNI	CCTNNNNNAGG	1 cut(s)	2921
EcoO109I	RGGNCCY	1 cut(s)	2209
EcoRI	GAATTC	1 cut(s)	262
EcoRII	CCWGG	5 cut(s)	276, 983, 1138, 1360, 1485
EcoT14I	CCWWGG	5 cut(s)	350, 1321, 2088, 2428, 2484
EcoT22I	ATGCAT	3 cut(s)	711, 1716, 2770
EcoT38I	GRGCYC	1 cut(s)	2820
ErhI	CCWWGG	5 cut(s)	350, 1321, 2088, 2428, 2484
FaqI	GGGAC	3 cut(s)	70, 307, 346
FauI	CCCGC	1 cut(s)	2604
FauNDI	CATATG	1 cut(s)	1221
FbaI	TGATCA	1 cut(s)	2254
FokI	GGATG	9 cut(s)	323, 512, 762, 881, 1701, 2030, 2066, 2572, 2732
FriOI	GRGCYC	1 cut(s)	2820
FspBI	CTAG	7 cut(s)	306, 1187, 1329, 1464, 1973, 2241, 2531
GlaI	GCGC	4 cut(s)	660, 1641, 1643, 2325
GsaI	CCCAGC	1 cut(s)	1013
GsuI	CTGGAG	5 cut(s)	73, 180, 297, 827, 2154
HaeIII	GGCC	7 cut(s)	569, 605, 791, 1485, 1698, 1780, 2603
HapII	CCGG	3 cut(s)	680, 1197, 1794
HgaI	GACGC	1 cut(s)	348
HhaI	GCGC	4 cut(s)	661, 1642, 1644, 2326
Hin6I	GCGC	4 cut(s)	659, 1640, 1642, 2324
HinP1I	GCGC	4 cut(s)	659, 1640, 1642, 2324
HincII	GTYRAC	2 cut(s)	1947, 2122
HindII	GTYRAC	2 cut(s)	1947, 2122
HindIII	AAGCTT	3 cut(s)	1583, 1850, 2854
HpaI	GTTAAC	1 cut(s)	1947
HpaII	CCGG	3 cut(s)	680, 1197, 1794
HphI	GGTGA	8 cut(s)	280, 290, 739, 2007, 2287, 2380, 2486, 2918
Hpy166II	GTNNAC	9 cut(s)	778, 1085, 1947, 2122, 2330, 2388, 2567, 2671, 2687
Hpy8I	GTNNAC	9 cut(s)	778, 1085, 1947, 2122, 2330, 2388, 2567, 2671, 2687
Hpy99I	CGWCG	1 cut(s)	251
HpyCH4III	ACNGT	6 cut(s)	818, 1968, 2026, 2287, 2553, 2558
HpyCH4IV	ACGT	4 cut(s)	910, 2452, 2775, 2882
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 656, 1574, 2237, 2402, 2463
HpySE526I	ACGT	4 cut(s)	910, 2452, 2775, 2882
HspAI	GCGC	4 cut(s)	659, 1640, 1642, 2324
Kpn2I	TCCGGA	1 cut(s)	1793
KpnI	GGTACC	1 cut(s)	284
KroI	GCCGGC	1 cut(s)	679
KroNI	GCCGGC	1 cut(s)	681
Ksp22I	TGATCA	1 cut(s)	2254
KspAI	GTTAAC	1 cut(s)	1947
Kzo9I	GATC	8 cut(s)	1180, 1325, 1492, 1741, 1957, 2254, 2263, 2779
LmnI	GCTCC	3 cut(s)	550, 616, 2815
LweI	GCATC	3 cut(s)	1362, 1464, 1723
MaeI	CTAG	7 cut(s)	306, 1187, 1329, 1464, 1973, 2241, 2531
MaeII	ACGT	4 cut(s)	910, 2452, 2775, 2882
MaeIII	GTNAC	6 cut(s)	1613, 2308, 2413, 2618, 2771, 2906
MalI	GATC	8 cut(s)	1182, 1327, 1494, 1743, 1959, 2256, 2265, 2781
MboI	GATC	8 cut(s)	1180, 1325, 1492, 1741, 1957, 2254, 2263, 2779
MfeI	CAATTG	2 cut(s)	1070, 2950
MflI	RGATCY	3 cut(s)	1325, 1957, 2779
MhlI	GDGCHC	3 cut(s)	780, 1290, 2820
MlsI	TGGCCA	1 cut(s)	791
MluNI	TGGCCA	1 cut(s)	791
MlyI	GAGTC	7 cut(s)	221, 422, 1764, 2253, 2518, 2744, 2755
MmeI	TCCRAC	4 cut(s)	53, 147, 153, 2498
Mox20I	TGGCCA	1 cut(s)	791
Mph1103I	ATGCAT	3 cut(s)	711, 1716, 2770
MroI	TCCGGA	1 cut(s)	1793
MroNI	GCCGGC	1 cut(s)	679
MroXI	GAANNNNTTC	1 cut(s)	1503
MscI	TGGCCA	1 cut(s)	791
MslI	CAYNNNNRTG	5 cut(s)	785, 1893, 2052, 2696, 2769
Msp20I	TGGCCA	1 cut(s)	791
MspA1I	CMGCKG	2 cut(s)	2114, 2611
MspCI	CTTAAG	1 cut(s)	1625
MspI	CCGG	3 cut(s)	680, 1197, 1794
MspR9I	CCNGG	5 cut(s)	278, 985, 1140, 1362, 1487
MunI	CAATTG	2 cut(s)	1070, 2950
Mva1269I	GAATGC	2 cut(s)	1600, 1874
MvaI	CCWGG	5 cut(s)	278, 985, 1140, 1362, 1487
MvnI	CGCG	3 cut(s)	1640, 1642, 2326
MwoI	GCNNNNNNNGC	6 cut(s)	11, 656, 1574, 2237, 2402, 2463
NaeI	GCCGGC	1 cut(s)	681
NcoI	CCATGG	2 cut(s)	350, 1321
NdeI	CATATG	1 cut(s)	1221
NdeII	GATC	8 cut(s)	1180, 1325, 1492, 1741, 1957, 2254, 2263, 2779
NgoMIV	GCCGGC	1 cut(s)	679
NlaIV	GGNNCC	4 cut(s)	282, 546, 1251, 2211
NmeAIII	GCCGAG	1 cut(s)	1724
NmuCI	GTSAC	4 cut(s)	1613, 2413, 2771, 2906
NsiI	ATGCAT	3 cut(s)	711, 1716, 2770
NspI	RCATGY	1 cut(s)	2772
PaeR7I	CTCGAG	1 cut(s)	257
PasI	CCCWGGG	1 cut(s)	984
PauI	GCGCGC	1 cut(s)	1640
PceI	AGGCCT	2 cut(s)	569, 1485
PcsI	WCGNNNNNNNCGW	1 cut(s)	255
PctI	GAATGC	2 cut(s)	1600, 1874
PdiI	GCCGGC	1 cut(s)	681
PdmI	GAANNNNTTC	1 cut(s)	1503
PfeI	GAWTC	8 cut(s)	317, 330, 739, 811, 1228, 1726, 1813, 1907
PflFI	GACNNNGTC	1 cut(s)	230
PflMI	CCANNNNNTGG	1 cut(s)	1263
PfoI	TCCNGGA	1 cut(s)	1360
PleI	GAGTC	7 cut(s)	221, 421, 1763, 2252, 2518, 2744, 2755
PmaCI	CACGTG	1 cut(s)	2453
PmlI	CACGTG	1 cut(s)	2453
PpsI	GAGTC	7 cut(s)	221, 421, 1763, 2252, 2518, 2744, 2755
Ppu21I	YACGTR	1 cut(s)	2453
PpuMI	RGGWCCY	1 cut(s)	2209
PshBI	ATTAAT	1 cut(s)	1425
PsiI	TTATAA	2 cut(s)	2366, 2579
Psp124BI	GAGCTC	1 cut(s)	2820
Psp5II	RGGWCCY	1 cut(s)	2209
Psp6I	CCWGG	5 cut(s)	276, 983, 1138, 1360, 1485
PspCI	CACGTG	1 cut(s)	2453
PspFI	CCCAGC	1 cut(s)	1009
PspGI	CCWGG	5 cut(s)	276, 983, 1138, 1360, 1485
PspN4I	GGNNCC	4 cut(s)	282, 546, 1251, 2211
PspPI	GGNCC	2 cut(s)	1082, 2209
PspPPI	RGGWCCY	1 cut(s)	2209
PstI	CTGCAG	2 cut(s)	2119, 2717
PstNI	CAGNNNCTG	1 cut(s)	2599
PsuI	RGATCY	3 cut(s)	1325, 1957, 2779
PsyI	GACNNNGTC	1 cut(s)	230
PteI	GCGCGC	1 cut(s)	1640
PvuII	CAGCTG	1 cut(s)	2114
RsaI	GTAC	7 cut(s)	282, 1053, 1318, 1512, 2178, 2555, 2670
RsaNI	GTAC	7 cut(s)	281, 1052, 1317, 1511, 2177, 2554, 2669
RseI	CAYNNNNRTG	5 cut(s)	785, 1893, 2052, 2696, 2769
SacI	GAGCTC	1 cut(s)	2820
Sau3AI	GATC	8 cut(s)	1180, 1325, 1492, 1741, 1957, 2254, 2263, 2779
Sau96I	GGNCC	2 cut(s)	1082, 2209
SbfI	CCTGCAGG	1 cut(s)	2717
SchI	GAGTC	7 cut(s)	221, 422, 1764, 2253, 2518, 2744, 2755
ScrFI	CCNGG	5 cut(s)	278, 985, 1140, 1362, 1487
SdaI	CCTGCAGG	1 cut(s)	2717
SduI	GDGCHC	3 cut(s)	780, 1290, 2820
SfaNI	GCATC	3 cut(s)	1362, 1464, 1723
SfcI	CTRYAG	3 cut(s)	2115, 2713, 2821
Sfr274I	CTCGAG	1 cut(s)	257
SinI	GGWCC	2 cut(s)	1082, 2209
SlaI	CTCGAG	1 cut(s)	257
SmiMI	CAYNNNNRTG	5 cut(s)	785, 1893, 2052, 2696, 2769
SmlI	CTYRAG	3 cut(s)	257, 1625, 2837
SmoI	CTYRAG	3 cut(s)	257, 1625, 2837
Sse8387I	CCTGCAGG	1 cut(s)	2717
SseBI	AGGCCT	2 cut(s)	569, 1485
SspI	AATATT	1 cut(s)	1357
SspMI	CTAG	7 cut(s)	306, 1187, 1329, 1464, 1973, 2241, 2531
SstI	GAGCTC	1 cut(s)	2820
StuI	AGGCCT	2 cut(s)	569, 1485
StyD4I	CCNGG	5 cut(s)	276, 983, 1138, 1360, 1485
StyI	CCWWGG	5 cut(s)	350, 1321, 2088, 2428, 2484
TaaI	ACNGT	6 cut(s)	818, 1968, 2026, 2287, 2553, 2558
TaiI	ACGT	4 cut(s)	913, 2455, 2778, 2885
TaqI	TCGA	6 cut(s)	249, 258, 416, 426, 1395, 1750
TatI	WGTACW	2 cut(s)	1051, 2668
TauI	GCSGC	5 cut(s)	653, 664, 863, 1130, 1780
TfiI	GAWTC	8 cut(s)	317, 330, 739, 811, 1228, 1726, 1813, 1907
TscAI	CASTG	4 cut(s)	702, 1624, 1973, 2424
TseFI	GTSAC	4 cut(s)	1613, 2413, 2771, 2906
Tsp45I	GTSAC	4 cut(s)	1613, 2413, 2771, 2906
TspDTI	ATGAA	9 cut(s)	284, 325, 885, 992, 1664, 1805, 1845, 2049, 2801
TspGWI	ACGGA	1 cut(s)	220
TspRI	CASTG	4 cut(s)	702, 1624, 1973, 2424
Tth111I	GACNNNGTC	1 cut(s)	230
Van91I	CCANNNNNTGG	1 cut(s)	1263
Vha464I	CTTAAG	1 cut(s)	1625
VneI	GTGCAC	1 cut(s)	776
VpaK11BI	GGWCC	2 cut(s)	1082, 2209
VspI	ATTAAT	1 cut(s)	1425
XagI	CCTNNNNNAGG	1 cut(s)	2921
XapI	RAATTY	7 cut(s)	262, 272, 293, 1545, 1763, 2662, 2842
XceI	RCATGY	1 cut(s)	2772
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1076, 2450
XhoI	CTCGAG	1 cut(s)	257
XmnI	GAANNNNTTC	1 cut(s)	1503
XspI	CTAG	7 cut(s)	306, 1187, 1329, 1464, 1973, 2241, 2531
Zsp2I	ATGCAT	3 cut(s)	711, 1716, 2770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.